Carg20336-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20336-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein-serine/threonine phosphatase
LocationCarg_Chr18: 11831260 .. 11834152 (-)
Sequence length1579
RNA-Seq ExpressionCarg20336-RA
SyntenyCarg20336-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATGGTATACCTGCATCGTTTTGTCTGTCCAACAATGAACAATCTCTTTCCACAGCTGTGGGGGCAACATTCAAACTATCTCAAACTATGGTATCTGATCCGTTAACCCAATTAGAGGCCTTCCTTCATTCTCTTATCTTTCCCTCTTAATGTAATCTCCTTCACACAAGTATTTGATTTGAATTGGACTCAACTGGGCTTGGAGTTTCGATTCAGCAAATGGCTTTGTTGAGTCCTCGATTGCATAGATTCCGCTTATTAACCAAGCTCCACCAAGCCTCTGCCTCCAAACCCACTAACGCAAATCAGCTCACTGTCAGAAGAAAAACCGGATGCTGCTATGCCATAGCTGTTGATGCTCCATCTTCTTTAACAGATGCTGCGGGAATCAGATGGGGTTCGACCACTTTGCAAGGCTTGCGGGAAGAAATGGAGGACGATGCTGTTGTGCGGTCTGATGGTCTTAATGGCTTCTTGTTTGCTGCAGTGTTCGATGGCCATGGTGGCTGTTCCTCCGTCAAGTTCCTCAGGTGGGAACCCAACAGGGCATCTTGTGGTGAAATCTTTAGGATTTATGAACATATGTATTGCAGAGTGGAGGCTTCCTTCACAATATCATTGGTTTATTCATAGTGCCAAAAATAGATAATAAACAAGGCCTTTGATTGTGTAAATAATATGGCAATAGGGAGGAGCTGTACAAAGACTGCATAACAGCTGTACAAGGTGGTCTGCTGTTGAATGGAGGGGATTTTGAAGTGATCAAAGCGGCTTTGGAGAAGGCTTTTGATGATACTGATAAAAGATTACTCCCTTTGTAAGTGGAAAGTCCAGTTCTTCAGTTGGTTATATCCTCTCGGTGCAATTCTGTATTTGACGTAATTTTTGCTCAACTTTTCAAGGCTGGAGGCAGCTGGGAAGGAAGATGAATCTGGTTCCACTGCAACTGTTGCATTCATACGAAATGATGTTATGTTCATTTCACATGTTGGTGACTCTTGTGTTGTAAGCATCAACCTGGTGATCTTTATATCTCAGTTTCTTGGCATAAGAAACAGTTCTAAGCATTGTTATTCTCTTTCTTAGTTAGTTTTGGAACTCAGTTTTGTGGGACTGGTTCTTTTATGTGCCCTTCTACATTCTGTCATGTTTTTCAATTAAGATCTTCTAAATACAGAGCTGATTTGTTCATGCAGGTGTTGTCACGTTCCGGAGGAGCACAGGTATTGACGAGTTCTCATCGGCCTTATGGAAACAACACCACCTCACTGCAAGAAATCAGAAGAATTAGAGAAGCAGGTGGATGGGTGTGTACCTGTTTCAGTTAACTCTCTTACCCATTTCTTCCATATAAAACATAATCATTCGATGCTTGTGTATTTGACATTTAAACTCTTTGTTGCTCGCTGCCCTGTTTGAAACAGGAAAGGCAGCATATGCCTACGTTAATCTGCCATTCTTACTGTTGAAAAATGACTTTCAATATTATTTATCTATCAAAAGACCAAATCTTTTTTTGAGTCATTAAAAATTTAGATACTCATTATTGCAGCTTTTGGTGTCTTTAGATCGTTAACGGGAGAATCTGTGGAGACATCTCTGTATCCCGTGCATTTGGTGACATACGATTCAAGACGAAGAAATACGAGTTTGGTCCTTTTAACTGTCCTCTAATATTCTCGAGCTTCAGAAGGTTTCTTTTTGCATCTCCTTATGGTTATATTCTATATAACAGGATGCTGAGAAAAGGAGTTGAAGAAGGGAGATGGTCTGAAAAGTTTGTTTCTCGGTAGGTATGCTTTATGATTCCTCACTTGTATCTTTCTTATTTCATTTTTCAACGCTTCTTATTGTTTAGTATTACCAATTGATTCAATTAAGGAAGCAGTTTGACCATAAAATCTTTTTAACTCATCAGTGTACAGTTCAATGGAGATTTGGTAACCGCATCACCAGAAGTTCTTCAGCTTACCCTCGGATCAAATGTCGAGTTTGTACTGATAGCGTCGGATGGCCTATGGGATTACATGAATAGGTCATTTTTCGTATCTCAGTCATTTCTAGGCTTCAGTCTTTATGTCCTGAAGATTTATCAGATTGTCCATCAACTTCGATCTAGACAGACTAAATAAGTGCCATTTTCGGGTATTGTATCAGCTCCGAAGCGGTTAAGTTTGTTCGGAATCAATTGAGTCAGCATGGTGATGTTCAGGTGTACATCATGTTACTTGTAATTCTTTATTCTTTTACGCACTCTATTTCTTGCTGTCTCTGTTTGCTTATGGTTCAACTTCTAACTTCTCATTTACTTTGATTTTCAGTTAGCTTGTGAAGCCCTCGCACAAGCAGCCTTGGTAATACTCGCACAATTTGATTTGTTTAAAGCTTCTAATAACAAGAACTTGGTTTCCAGTTCTAATAAAGCAAATATTATGTTTGTATCTTGTAGGACAAAGGGTCACAAGACAATATCAGCATCATCATGGCTGATTTGGGGTGAGTACACTTAAGTATAAACATGGCAAATGAGGAGACAGTTATAATAGTAGGCATTGAAAGAATCAAAGTTCAAATGGCTTTGCTTGTTTCCAAATGCATAGAAACAATAAACTTTCTTCTGAGAAGCTAAATACAGCAGCTAACGAAACTCGAAACTCGATCTCAGTTATTAGACTGTCTTATTTGTTGTTGCGGGAGTTCTTCCAATACAAATATCATTTAAACTAACTGGAGATCAGATGCAGGCGGACAGATTGGCAGAATTTGCCCCTCCAGCAAGACAACGTCATATTTGAAGTGGCTCAAGCTTTTGGTACGATTGGGGTCGTTTCTTTCGGTATTTGGTGGGCGTCCTCTACTTTCTGTCTTTAAAGTTTGTGTGTCCCACTGTTG

mRNA sequence

TGATGGTATACCTGCATCGTTTTGTCTGTCCAACAATGAACAATCTCTTTCCACAGCTGTGGGGGCAACATTCAAACTATCTCAAACTATGGTATCTGATCCGTTAACCCAATTAGAGGCCTTCCTTCATTCTCTTATCTTTCCCTCTTAATGTAATCTCCTTCACACAAGTATTTGATTTGAATTGGACTCAACTGGGCTTGGAGTTTCGATTCAGCAAATGGCTTTGTTGAGTCCTCGATTGCATAGATTCCGCTTATTAACCAAGCTCCACCAAGCCTCTGCCTCCAAACCCACTAACGCAAATCAGCTCACTGTCAGAAGAAAAACCGGATGCTGCTATGCCATAGCTGTTGATGCTCCATCTTCTTTAACAGATGCTGCGGGAATCAGATGGGGTTCGACCACTTTGCAAGGCTTGCGGGAAGAAATGGAGGACGATGCTGTTGTGCGGTCTGATGGTCTTAATGGCTTCTTGTTTGCTGCAGTGTTCGATGGCCATGGTGGCTGTTCCTCCGTCAAGTTCCTCAGGGAGGAGCTGTACAAAGACTGCATAACAGCTGTACAAGGTGGTCTGCTGTTGAATGGAGGGGATTTTGAAGTGATCAAAGCGGCTTTGGAGAAGGCTTTTGATGATACTGATAAAAGATTACTCCCTTTGCTGGAGGCAGCTGGGAAGGAAGATGAATCTGGTTCCACTGCAACTGTTGCATTCATACGAAATGATGTTATGTTCATTTCACATGTTGGTGACTCTTGTGTTGTGTTGTCACGTTCCGGAGGAGCACAGGTATTGACGAGTTCTCATCGGCCTTATGGAAACAACACCACCTCACTGCAAGAAATCAGAAGAATTAGAGAAGCAGGTGGATGGATCGTTAACGGGAGAATCTGTGGAGACATCTCTGTATCCCGTGCATTTGGTGACATACGATTCAAGACGAAGAAATACGAGTTTGGTCCTTTTAACTGTCCTCTAATATTCTCGAGCTTCAGAAGGTTTCTTTTTGCATCTCCTTATGGTTATATTCTATATAACAGGATGCTGAGAAAAGGAGTTGAAGAAGGGAGATGGTCTGAAAAGTTTGTTTCTCGTGTACAGTTCAATGGAGATTTGGTAACCGCATCACCAGAAGTTCTTCAGCTTACCCTCGGATCAAATGTCGAGTTTGTACTGATAGCGTCGGATGGCCTATGGGATTACATGAATAGCTCCGAAGCGGTTAAGTTTGTTCGGAATCAATTGAGTCAGCATGGTGATGTTCAGTTAGCTTGTGAAGCCCTCGCACAAGCAGCCTTGGTAATACTCGCACAATTTGATTTGTTTAAAGCTTCTAATAACAAGAACTTGGTTTCCAGTTCTAATAAAGCAAATATTATGTTTGACAAAGGGTCACAAGACAATATCAGCATCATCATGGCTGATTTGGGGCGGACAGATTGGCAGAATTTGCCCCTCCAGCAAGACAACGTCATATTTGAAGTGGCTCAAGCTTTTGGTACGATTGGGGTCGTTTCTTTCGGTATTTGGTGGGCGTCCTCTACTTTCTGTCTTTAAAGTTTGTGTGTCCCACTGTTG

Coding sequence (CDS)

ATGGCTTTGTTGAGTCCTCGATTGCATAGATTCCGCTTATTAACCAAGCTCCACCAAGCCTCTGCCTCCAAACCCACTAACGCAAATCAGCTCACTGTCAGAAGAAAAACCGGATGCTGCTATGCCATAGCTGTTGATGCTCCATCTTCTTTAACAGATGCTGCGGGAATCAGATGGGGTTCGACCACTTTGCAAGGCTTGCGGGAAGAAATGGAGGACGATGCTGTTGTGCGGTCTGATGGTCTTAATGGCTTCTTGTTTGCTGCAGTGTTCGATGGCCATGGTGGCTGTTCCTCCGTCAAGTTCCTCAGGGAGGAGCTGTACAAAGACTGCATAACAGCTGTACAAGGTGGTCTGCTGTTGAATGGAGGGGATTTTGAAGTGATCAAAGCGGCTTTGGAGAAGGCTTTTGATGATACTGATAAAAGATTACTCCCTTTGCTGGAGGCAGCTGGGAAGGAAGATGAATCTGGTTCCACTGCAACTGTTGCATTCATACGAAATGATGTTATGTTCATTTCACATGTTGGTGACTCTTGTGTTGTGTTGTCACGTTCCGGAGGAGCACAGGTATTGACGAGTTCTCATCGGCCTTATGGAAACAACACCACCTCACTGCAAGAAATCAGAAGAATTAGAGAAGCAGGTGGATGGATCGTTAACGGGAGAATCTGTGGAGACATCTCTGTATCCCGTGCATTTGGTGACATACGATTCAAGACGAAGAAATACGAGTTTGGTCCTTTTAACTGTCCTCTAATATTCTCGAGCTTCAGAAGGTTTCTTTTTGCATCTCCTTATGGTTATATTCTATATAACAGGATGCTGAGAAAAGGAGTTGAAGAAGGGAGATGGTCTGAAAAGTTTGTTTCTCGTGTACAGTTCAATGGAGATTTGGTAACCGCATCACCAGAAGTTCTTCAGCTTACCCTCGGATCAAATGTCGAGTTTGTACTGATAGCGTCGGATGGCCTATGGGATTACATGAATAGCTCCGAAGCGGTTAAGTTTGTTCGGAATCAATTGAGTCAGCATGGTGATGTTCAGTTAGCTTGTGAAGCCCTCGCACAAGCAGCCTTGGTAATACTCGCACAATTTGATTTGTTTAAAGCTTCTAATAACAAGAACTTGGTTTCCAGTTCTAATAAAGCAAATATTATGTTTGACAAAGGGTCACAAGACAATATCAGCATCATCATGGCTGATTTGGGGCGGACAGATTGGCAGAATTTGCCCCTCCAGCAAGACAACGTCATATTTGAAGTGGCTCAAGCTTTTGGTACGATTGGGGTCGTTTCTTTCGGTATTTGGTGGGCGTCCTCTACTTTCTGTCTTTAA

Protein sequence

MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWGSTTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLLLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAALVILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIFEVAQAFGTIGVVSFGIWWASSTFCL
Homology
BLAST of Carg20336-RA vs. NCBI nr
Match: KAG7013313.1 (Protein phosphatase 2C 57 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 885.9 bits (2288), Expect = 1.4e-253
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL
Sbjct: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV
Sbjct: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL
Sbjct: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
           VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           EVAQAFGTIGVVSFGIWWASSTFCL
Sbjct: 421 EVAQAFGTIGVVSFGIWWASSTFCL 445

BLAST of Carg20336-RA vs. NCBI nr
Match: XP_023541443.1 (protein phosphatase 2C 57-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739.2 bits (1907), Expect = 2.1e-209
Identity = 385/445 (86.52%), Postives = 387/445 (86.97%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDC+TAVQGGLL
Sbjct: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCLTAVQGGLL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKKYE                             MLRKGVEEGRWSEKFVSRVQFNGDLV
Sbjct: 241 TKKYE-----------------------------MLRKGVEEGRWSEKFVSRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPEVLQLTLGSNVEFVLIASDGLWDYMNSS+AVKFVRNQLSQHGDVQLACEALAQAAL
Sbjct: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSDAVKFVRNQLSQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 388

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           EVAQAFGTIGVVSFGIWWASS FCL
Sbjct: 421 EVAQAFGTIGVVSFGIWWASSIFCL 388

BLAST of Carg20336-RA vs. NCBI nr
Match: XP_022945263.1 (protein phosphatase 2C 57-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 738.8 bits (1906), Expect = 2.8e-209
Identity = 385/445 (86.52%), Postives = 386/445 (86.74%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGL+
Sbjct: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLM 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKKYE                             MLRKG EEGRWSEKFVSRVQFNGDLV
Sbjct: 241 TKKYE-----------------------------MLRKGAEEGRWSEKFVSRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL
Sbjct: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 388

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           EVAQAFGTIGVVSFGIWWASS FCL
Sbjct: 421 EVAQAFGTIGVVSFGIWWASSIFCL 388

BLAST of Carg20336-RA vs. NCBI nr
Match: KAG6574245.1 (Protein phosphatase 2C 57, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 736.5 bits (1900), Expect = 1.4e-208
Identity = 385/445 (86.52%), Postives = 385/445 (86.52%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL
Sbjct: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNNT SLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNTISLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKKYE                             MLRKGVEEGRWSEKFVSRVQFNGDLV
Sbjct: 241 TKKYE-----------------------------MLRKGVEEGRWSEKFVSRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL
Sbjct: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 388

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           EVAQAFG IGVVSFGIWWASS FCL
Sbjct: 421 EVAQAFGMIGVVSFGIWWASSIFCL 388

BLAST of Carg20336-RA vs. NCBI nr
Match: XP_022968153.1 (protein phosphatase 2C 57-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 727.2 bits (1876), Expect = 8.3e-206
Identity = 379/445 (85.17%), Postives = 383/445 (86.07%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFRLLTKLHQASASKPT ANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFRLLTKLHQASASKPTKANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL
Sbjct: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFD TDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDGTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNNT SLQEIRRIREAGGWIV+GRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNTASLQEIRRIREAGGWIVDGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKKYE                             MLRKGVEEGRWSEKFVSRVQFNGDLV
Sbjct: 241 TKKYE-----------------------------MLRKGVEEGRWSEKFVSRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPEVLQLTLGSNVEF+LIASDGLWDYMNS +AVKFVRNQLSQHGDVQLACEALAQAAL
Sbjct: 301 TASPEVLQLTLGSNVEFILIASDGLWDYMNSFDAVKFVRNQLSQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVI+
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIY 388

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           EVAQAFGTIGVVSFGIWWASS FCL
Sbjct: 421 EVAQAFGTIGVVSFGIWWASSIFCL 388

BLAST of Carg20336-RA vs. ExPASy Swiss-Prot
Match: P49599 (Protein phosphatase 2C 57 OS=Arabidopsis thaliana OX=3702 GN=PPH1 PE=1 SV=2)

HSP 1 Score: 468.4 bits (1204), Expect = 9.1e-131
Identity = 248/443 (55.98%), Postives = 303/443 (68.40%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTG--CCYAIAVDAPSSLTDAAGIR 60
           MALL P LHRF   T  H A  S       L V    G   C AIA+DAPSSLT    IR
Sbjct: 1   MALLRPHLHRFHSNTLRHSAYPSADAGGG-LVVYPTYGRHRCSAIAIDAPSSLTGVTPIR 60

Query: 61  WGSTTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGG 120
           WG T++QG R+EMEDD V+RSD ++ F +AAVFDGH G SSVKFLREELYK+C+ A+Q G
Sbjct: 61  WGYTSVQGFRDEMEDDIVIRSDAVDSFSYAAVFDGHAGSSSVKFLREELYKECVGALQAG 120

Query: 121 LLLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAG-KEDESGSTATVAFIRNDVMFISHVG 180
            LLNGGDF  IK AL KAF+  D+ LL  LEA G +EDESGSTATV  IRNDV FI+H+G
Sbjct: 121 SLLNGGDFAAIKEALIKAFESVDRNLLKWLEANGDEEDESGSTATVMIIRNDVSFIAHIG 180

Query: 181 DSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDI 240
           DSC VLSRSG  + LT  HRPYG++  ++QE++R++EAGGWIVNGRICGDI+VSRAFGDI
Sbjct: 181 DSCAVLSRSGQIEELTDYHRPYGSSRAAIQEVKRVKEAGGWIVNGRICGDIAVSRAFGDI 240

Query: 241 RFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNG 300
           RFKTKK                             N ML+KGV+EGRWSEKFVSR++F G
Sbjct: 241 RFKTKK-----------------------------NDMLKKGVDEGRWSEKFVSRIEFKG 300

Query: 301 DLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQ 360
           D+V A+P++ Q+ L S+VEF+++ASDGLWDYM SS+ V +VR+QL +HG+VQLACE+LAQ
Sbjct: 301 DMVVATPDIFQVPLTSDVEFIILASDGLWDYMKSSDVVSYVRDQLRKHGNVQLACESLAQ 360

Query: 361 AALVILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDN 420
            AL                            D+ SQDNISII+ADLGRT+W+NLP Q+ N
Sbjct: 361 VAL----------------------------DRRSQDNISIIIADLGRTEWKNLPAQRQN 385

Query: 421 VIFEVAQAFGTIGVVSFGIWWAS 441
           V+ E+ QA  TIG+V+ GIW +S
Sbjct: 421 VVVELVQAATTIGLVTVGIWMSS 385

BLAST of Carg20336-RA vs. ExPASy Swiss-Prot
Match: Q5JKN1 (Probable protein phosphatase 2C 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0552300 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 8.3e-108
Identity = 214/442 (48.42%), Postives = 277/442 (62.67%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPR+ R   L     A A      +         C  + A    SS ++   IRWG
Sbjct: 1   MALLSPRVPRLP-LASASAAGAGLRCCVSGGRAGSAAWCHASAAGSVASSSSELEAIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDG-LNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGL 120
           +  LQG R+EMED+ V+R    L+GF FAAVFDGH G S+V+FLR+ELYK+C  A+ GG 
Sbjct: 61  TAKLQGARDEMEDEVVLRPGSLLDGFSFAAVFDGHAGFSAVEFLRDELYKECAAALDGGA 120

Query: 121 LLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDS 180
           +L+  + + I  ++++AF   D  L   LE   KEDESG+TAT  F+RNDV+ +SH+GDS
Sbjct: 121 VLSTKNLDAITDSIQRAFATVDANLSTWLEQMDKEDESGATATAMFLRNDVLVVSHIGDS 180

Query: 181 CV-VLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIR 240
           C+ V+SR G  Q +T+ HRPYGN   SL+E++RIR AGGWIV+GRICG+ISVSRAFGDIR
Sbjct: 181 CLQVVSRGGRPQAVTNFHRPYGNKKASLEEVKRIRAAGGWIVDGRICGEISVSRAFGDIR 240

Query: 241 FKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGD 300
           FKT+K                             N ML KGV+EGRW+EKF+SR+ F GD
Sbjct: 241 FKTRK-----------------------------NEMLVKGVKEGRWTEKFISRINFKGD 300

Query: 301 LVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQA 360
           L+ +SP+V  + LG +VEFVL+A+DGLWDY+ SSEAV  VR+QL QHGDVQ+ACEAL Q 
Sbjct: 301 LIVSSPDVSLVELGPDVEFVLLATDGLWDYIKSSEAVALVRDQLRQHGDVQVACEALGQI 360

Query: 361 ALVILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNV 420
           AL                            D+ SQDNISI++ADLGRT+W+ LP Q+ N+
Sbjct: 361 AL----------------------------DRRSQDNISIVIADLGRTNWKELPAQRPNL 384

Query: 421 IFEVAQAFGTIGVVSFGIWWAS 441
             E+ QA  T+G VS GI+ +S
Sbjct: 421 FLELTQAVATVGAVSLGIYISS 384

BLAST of Carg20336-RA vs. ExPASy Swiss-Prot
Match: Q8VZN9 (Probable protein phosphatase 2C 11 OS=Arabidopsis thaliana OX=3702 GN=At1g43900 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.0e-25
Identity = 101/348 (29.02%), Postives = 162/348 (46.55%), Query Frame = 0

Query: 21  SASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIR-----WGSTTLQGLREEMEDDA 80
           S+S P   + L +  +       A+ +   ++  AG+R     +G ++L+G R  MED  
Sbjct: 85  SSSSPATKSSLMISSRD----PNALFSGGGISFLAGVRTVKFSYGYSSLKGKRATMEDYF 144

Query: 81  VVRSDGLNGFL--FAAVFDGHGGCSSVKFLREELYKDCITAVQGGLLLNGGDF-EVIKAA 140
             R   +NG +  F  VFDGHGG  + ++L+  L+K+         L++  DF    K A
Sbjct: 145 ETRISDVNGQMVAFFGVFDGHGGARTAEYLKNNLFKN---------LVSHDDFISDTKKA 204

Query: 141 LEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSCVVLSRSGGAQVL 200
           + + F  TD+    L+E AG+   +GSTA  AF+  D + +++VGDS VV SR+G A  L
Sbjct: 205 IVEVFKQTDEEY--LIEEAGQPKNAGSTAATAFLIGDKLIVANVGDSRVVASRNGSAVPL 264

Query: 201 TSSHRPYGNNTTSLQEIRRIREAGGWIV---NGRICGDISVSRAFGDIRFKTKKYEFGPF 260
           +  H+P  ++     E +RI +AGG+I+     R+ G ++VSRAFGD + K         
Sbjct: 265 SDDHKPDRSD-----ERQRIEDAGGFIIWAGTWRVGGILAVSRAFGDKQLK--------- 324

Query: 261 NCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLVTASPEVLQL 320
                           PY                                V A PE+ + 
Sbjct: 325 ----------------PY--------------------------------VIAEPEIQEE 350

Query: 321 TLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQ 358
            + S +EF+++ASDGLW+ +++ +AV  VR+      D + A   L Q
Sbjct: 385 DI-STLEFIVVASDGLWNVLSNKDAVAIVRD----ISDAETAARKLVQ 350

BLAST of Carg20336-RA vs. ExPASy Swiss-Prot
Match: P49596 (Probable protein phosphatase 2C T23F11.1 OS=Caenorhabditis elegans OX=6239 GN=ppm-2 PE=3 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 3.9e-25
Identity = 91/303 (30.03%), Postives = 140/303 (46.20%), Query Frame = 0

Query: 60  GSTTLQGLREEMED--DAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQG 119
           GS+ +QG R +MED    ++         F AV+DGHGG    ++    L+K  +   + 
Sbjct: 25  GSSCMQGWRVDMEDAHTHLLSLPDDPKCAFFAVYDGHGGSKVSQYSGINLHKKVVAQKE- 84

Query: 120 GLLLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVG 179
               + G+   +K A+EK F + D+++   ++   K+D SG+TA V  I+   ++  + G
Sbjct: 85  ---FSEGN---MKEAIEKGFLELDQQM--RVDEETKDDVSGTTAVVVLIKEGDVYCGNAG 144

Query: 180 DSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDI 239
           DS  V S  G A+ L+  H+P     +   E RRI  AGGW+   R+ G++++SRA GD 
Sbjct: 145 DSRAVSSVVGEARPLSFDHKP-----SHETEARRIIAAGGWVEFNRVNGNLALSRALGDF 204

Query: 240 RFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNG 299
            FK         NC                          K  EE               
Sbjct: 205 AFK---------NCD------------------------TKPAEE--------------- 264

Query: 300 DLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQ 359
            +VTA P+V+   L  + EF+++A DG+WD M + E V FVR +L++  D Q  CE L  
Sbjct: 265 QIVTAFPDVITDKLTPDHEFIVLACDGIWDVMTNQEVVDFVREKLAEKRDPQSICEELLT 265

Query: 360 AAL 361
             L
Sbjct: 325 RCL 265

BLAST of Carg20336-RA vs. ExPASy Swiss-Prot
Match: Q8BGL1 (Probable protein phosphatase 1N OS=Mus musculus OX=10090 GN=Ppm1n PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.3e-24
Identity = 93/309 (30.10%), Postives = 134/309 (43.37%), Query Frame = 0

Query: 55  AGIRWGSTTLQGLREEMEDDAVVR--SDGL-NGFLFAAVFDGHGGCSSVKFLREELYKDC 114
           +G+R+G++ +QG R  MED    R    GL +G+ F AV DGHGG  + +F    L    
Sbjct: 56  SGLRFGASAVQGWRARMEDAHCARLALPGLPSGWAFFAVLDGHGGARAARFGARHL---- 115

Query: 115 ITAVQGGLLLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVM 174
              V G L     + + ++ AL  AF   D +L  L     + D  GSTA    +    +
Sbjct: 116 PGYVLGELGPAPQEPDGVRQALRSAFLQADAQLSALWP---RGDPGGSTAVALLVSPRFL 175

Query: 175 FISHVGDSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVS 234
           +++H GDS  +LSRSG     T  HRP+       +E  RI +AGG +   R+ G ++VS
Sbjct: 176 YLAHCGDSRALLSRSGSVAFCTEDHRPH-----RPRERERIHDAGGTVRRRRVEGSLAVS 235

Query: 235 RAFGDIRFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVS 294
           RA GD  +K                                          GR  E    
Sbjct: 236 RALGDFAYKQ---------------------------------------APGRPPEL--- 295

Query: 295 RVQFNGDLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLA 354
                  LV+A PEV  L      EFVL+ASDG+WD ++ ++    V ++L    D++L 
Sbjct: 296 ------QLVSAEPEVAALARQDEDEFVLLASDGVWDALSGADLAGLVTSRLRLGLDLELL 304

Query: 355 CEALAQAAL 361
           C  L    L
Sbjct: 356 CAQLLDTCL 304

BLAST of Carg20336-RA vs. ExPASy TrEMBL
Match: A0A6J1G0B7 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111449563 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.3e-209
Identity = 385/445 (86.52%), Postives = 386/445 (86.74%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGL+
Sbjct: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLM 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKKYE                             MLRKG EEGRWSEKFVSRVQFNGDLV
Sbjct: 241 TKKYE-----------------------------MLRKGAEEGRWSEKFVSRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL
Sbjct: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 388

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           EVAQAFGTIGVVSFGIWWASS FCL
Sbjct: 421 EVAQAFGTIGVVSFGIWWASSIFCL 388

BLAST of Carg20336-RA vs. ExPASy TrEMBL
Match: A0A6J1HX68 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111467474 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.0e-206
Identity = 379/445 (85.17%), Postives = 383/445 (86.07%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFRLLTKLHQASASKPT ANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFRLLTKLHQASASKPTKANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL
Sbjct: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFD TDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDGTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNNT SLQEIRRIREAGGWIV+GRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNTASLQEIRRIREAGGWIVDGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKKYE                             MLRKGVEEGRWSEKFVSRVQFNGDLV
Sbjct: 241 TKKYE-----------------------------MLRKGVEEGRWSEKFVSRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPEVLQLTLGSNVEF+LIASDGLWDYMNS +AVKFVRNQLSQHGDVQLACEALAQAAL
Sbjct: 301 TASPEVLQLTLGSNVEFILIASDGLWDYMNSFDAVKFVRNQLSQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVI+
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIY 388

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           EVAQAFGTIGVVSFGIWWASS FCL
Sbjct: 421 EVAQAFGTIGVVSFGIWWASSIFCL 388

BLAST of Carg20336-RA vs. ExPASy TrEMBL
Match: A0A5D3CM48 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001490 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 8.7e-185
Identity = 343/445 (77.08%), Postives = 364/445 (81.80%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFR LTKLH  SASKPT   +LT+R+K  CC AIA+DAPSSLT AAGIRWG
Sbjct: 1   MALLSPRLHRFR-LTKLHHVSASKPTKTTKLTLRKKARCCSAIAIDAPSSLTGAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVV SDG N FLFAAVFDGHGG SSVKFLREELYKDC+ A+QGGLL
Sbjct: 61  STTLQGLREEMEDDAVVWSDGFNDFLFAAVFDGHGGYSSVKFLREELYKDCVAALQGGLL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLL LLEAAG+EDESG+TATVAFIRNDV+FISH+GDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLLLLEAAGEEDESGATATVAFIRNDVLFISHLGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNN+TSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNSTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKK E                             ML+KGVEEGRWSEKF+SRVQFNGDLV
Sbjct: 241 TKKSE-----------------------------MLQKGVEEGRWSEKFISRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPE+ Q+TLGSN EFVL+ASDGLWDYMNSS+AV FVRN+L QHGDVQLACEALAQAAL
Sbjct: 301 TASPEIFQVTLGSNAEFVLMASDGLWDYMNSSDAVGFVRNELRQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 387

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           E+AQAF TIG+VS GIWWASS+F L
Sbjct: 421 ELAQAFATIGLVSLGIWWASSSFSL 387

BLAST of Carg20336-RA vs. ExPASy TrEMBL
Match: A0A1S3C298 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103496050 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 8.7e-185
Identity = 343/445 (77.08%), Postives = 364/445 (81.80%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFR LTKLH  SASKPT   +LT+R+K  CC AIA+DAPSSLT AAGIRWG
Sbjct: 1   MALLSPRLHRFR-LTKLHHVSASKPTKTTKLTLRKKARCCSAIAIDAPSSLTGAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STTLQGLREEMEDDAVV SDG N FLFAAVFDGHGG SSVKFLREELYKDC+ A+QGGLL
Sbjct: 61  STTLQGLREEMEDDAVVWSDGFNDFLFAAVFDGHGGYSSVKFLREELYKDCVAALQGGLL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLL LLEAAG+EDESG+TATVAFIRNDV+FISH+GDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLLLLEAAGEEDESGATATVAFIRNDVLFISHLGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNN+TSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNSTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKK E                             ML+KGVEEGRWSEKF+SRVQFNGDLV
Sbjct: 241 TKKSE-----------------------------MLQKGVEEGRWSEKFISRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPE+ Q+TLGSN EFVL+ASDGLWDYMNSS+AV FVRN+L QHGDVQLACEALAQAAL
Sbjct: 301 TASPEIFQVTLGSNAEFVLMASDGLWDYMNSSDAVGFVRNELRQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 ----------------------------DKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 387

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           E+AQAF TIG+VS GIWWASS+F L
Sbjct: 421 ELAQAFATIGLVSLGIWWASSSFSL 387

BLAST of Carg20336-RA vs. ExPASy TrEMBL
Match: A0A0A0L799 (Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_3G238190 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.5e-184
Identity = 340/445 (76.40%), Postives = 364/445 (81.80%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIRWG 60
           MALLSPRLHRFR LTKLH  SASKPT A +LT+R+ + CC AIA+DAPSSLTDAAGIRWG
Sbjct: 1   MALLSPRLHRFR-LTKLHHVSASKPTKATKLTLRKNSRCCSAIAIDAPSSLTDAAGIRWG 60

Query: 61  STTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGGLL 120
           STT QGLREEMEDDAVVRSDG N FLFAAVFDGHGG SSVKFLREELYKDC+ A+QGG L
Sbjct: 61  STTFQGLREEMEDDAVVRSDGFNDFLFAAVFDGHGGYSSVKFLREELYKDCVAALQGGQL 120

Query: 121 LNGGDFEVIKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSC 180
           LNGGDFEVIKAALEKAFDDTDKRLL LLEAAG+EDESG+TATVAFIRNDV+FISH+GDSC
Sbjct: 121 LNGGDFEVIKAALEKAFDDTDKRLLLLLEAAGEEDESGATATVAFIRNDVLFISHLGDSC 180

Query: 181 VVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240
           VVLSRSGGAQVLTSSHRPYGNN+TSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK
Sbjct: 181 VVLSRSGGAQVLTSSHRPYGNNSTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDIRFK 240

Query: 241 TKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLV 300
           TKK E                             ML+KGVEEGRWSEKF+SRVQFNGDLV
Sbjct: 241 TKKSE-----------------------------MLQKGVEEGRWSEKFISRVQFNGDLV 300

Query: 301 TASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQAAL 360
           TASPE+ Q+TLGSN EFVL+ASDGLWDYMNSS+AV FVRN+L QHGDVQLACEALAQAAL
Sbjct: 301 TASPEIFQVTLGSNAEFVLMASDGLWDYMNSSDAVMFVRNELRQHGDVQLACEALAQAAL 360

Query: 361 VILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDNVIF 420
                                       DKGSQDN+SIIMADLGRTDWQNLPLQQDNVIF
Sbjct: 361 ----------------------------DKGSQDNVSIIMADLGRTDWQNLPLQQDNVIF 387

Query: 421 EVAQAFGTIGVVSFGIWWASSTFCL 446
           E+AQA  T+G+VS GIWWASS+F L
Sbjct: 421 ELAQALATMGLVSLGIWWASSSFSL 387

BLAST of Carg20336-RA vs. TAIR 10
Match: AT4G27800.1 (thylakoid-associated phosphatase 38 )

HSP 1 Score: 468.4 bits (1204), Expect = 6.5e-132
Identity = 248/443 (55.98%), Postives = 303/443 (68.40%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTG--CCYAIAVDAPSSLTDAAGIR 60
           MALL P LHRF   T  H A  S       L V    G   C AIA+DAPSSLT    IR
Sbjct: 1   MALLRPHLHRFHSNTLRHSAYPSADAGGG-LVVYPTYGRHRCSAIAIDAPSSLTGVTPIR 60

Query: 61  WGSTTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGG 120
           WG T++QG R+EMEDD V+RSD ++ F +AAVFDGH G SSVKFLREELYK+C+ A+Q G
Sbjct: 61  WGYTSVQGFRDEMEDDIVIRSDAVDSFSYAAVFDGHAGSSSVKFLREELYKECVGALQAG 120

Query: 121 LLLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAG-KEDESGSTATVAFIRNDVMFISHVG 180
            LLNGGDF  IK AL KAF+  D+ LL  LEA G +EDESGSTATV  IRNDV FI+H+G
Sbjct: 121 SLLNGGDFAAIKEALIKAFESVDRNLLKWLEANGDEEDESGSTATVMIIRNDVSFIAHIG 180

Query: 181 DSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDI 240
           DSC VLSRSG  + LT  HRPYG++  ++QE++R++EAGGWIVNGRICGDI+VSRAFGDI
Sbjct: 181 DSCAVLSRSGQIEELTDYHRPYGSSRAAIQEVKRVKEAGGWIVNGRICGDIAVSRAFGDI 240

Query: 241 RFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNG 300
           RFKTKK                             N ML+KGV+EGRWSEKFVSR++F G
Sbjct: 241 RFKTKK-----------------------------NDMLKKGVDEGRWSEKFVSRIEFKG 300

Query: 301 DLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQ 360
           D+V A+P++ Q+ L S+VEF+++ASDGLWDYM SS+ V +VR+QL +HG+VQLACE+LAQ
Sbjct: 301 DMVVATPDIFQVPLTSDVEFIILASDGLWDYMKSSDVVSYVRDQLRKHGNVQLACESLAQ 360

Query: 361 AALVILAQFDLFKASNNKNLVSSSNKANIMFDKGSQDNISIIMADLGRTDWQNLPLQQDN 420
            AL                            D+ SQDNISII+ADLGRT+W+NLP Q+ N
Sbjct: 361 VAL----------------------------DRRSQDNISIIIADLGRTEWKNLPAQRQN 385

Query: 421 VIFEVAQAFGTIGVVSFGIWWAS 441
           V+ E+ QA  TIG+V+ GIW +S
Sbjct: 421 VVVELVQAATTIGLVTVGIWMSS 385

BLAST of Carg20336-RA vs. TAIR 10
Match: AT4G27800.2 (thylakoid-associated phosphatase 38 )

HSP 1 Score: 416.8 bits (1070), Expect = 2.2e-116
Identity = 216/364 (59.34%), Postives = 261/364 (71.70%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTG--CCYAIAVDAPSSLTDAAGIR 60
           MALL P LHRF   T  H A  S       L V    G   C AIA+DAPSSLT    IR
Sbjct: 1   MALLRPHLHRFHSNTLRHSAYPSADAGGG-LVVYPTYGRHRCSAIAIDAPSSLTGVTPIR 60

Query: 61  WGSTTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGG 120
           WG T++QG R+EMEDD V+RSD ++ F +AAVFDGH G SSVKFLREELYK+C+ A+Q G
Sbjct: 61  WGYTSVQGFRDEMEDDIVIRSDAVDSFSYAAVFDGHAGSSSVKFLREELYKECVGALQAG 120

Query: 121 LLLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAG-KEDESGSTATVAFIRNDVMFISHVG 180
            LLNGGDF  IK AL KAF+  D+ LL  LEA G +EDESGSTATV  IRNDV FI+H+G
Sbjct: 121 SLLNGGDFAAIKEALIKAFESVDRNLLKWLEANGDEEDESGSTATVMIIRNDVSFIAHIG 180

Query: 181 DSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDI 240
           DSC VLSRSG  + LT  HRPYG++  ++QE++R++EAGGWIVNGRICGDI+VSRAFGDI
Sbjct: 181 DSCAVLSRSGQIEELTDYHRPYGSSRAAIQEVKRVKEAGGWIVNGRICGDIAVSRAFGDI 240

Query: 241 RFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNG 300
           RFKTKK                             N ML+KGV+EGRWSEKFVSR++F G
Sbjct: 241 RFKTKK-----------------------------NDMLKKGVDEGRWSEKFVSRIEFKG 300

Query: 301 DLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQ 360
           D+V A+P++ Q+ L S+VEF+++ASDGLWDYM SS+ V +VR+QL +HG+VQLACE+LAQ
Sbjct: 301 DMVVATPDIFQVPLTSDVEFIILASDGLWDYMKSSDVVSYVRDQLRKHGNVQLACESLAQ 334

Query: 361 AALV 362
            ALV
Sbjct: 361 VALV 334

BLAST of Carg20336-RA vs. TAIR 10
Match: AT4G27800.3 (thylakoid-associated phosphatase 38 )

HSP 1 Score: 402.1 bits (1032), Expect = 5.7e-112
Identity = 207/355 (58.31%), Postives = 251/355 (70.70%), Query Frame = 0

Query: 1   MALLSPRLHRFRLLTKLHQASASKPTNANQLTVRRKTG--CCYAIAVDAPSSLTDAAGIR 60
           MALL P LHRF   T  H A  S       L V    G   C AIA+DAPSSLT    IR
Sbjct: 1   MALLRPHLHRFHSNTLRHSAYPSADAGGG-LVVYPTYGRHRCSAIAIDAPSSLTGVTPIR 60

Query: 61  WGSTTLQGLREEMEDDAVVRSDGLNGFLFAAVFDGHGGCSSVKFLREELYKDCITAVQGG 120
           WG T++QG R+EMEDD V+RSD ++ F +AAVFDGH G SSVKFLREELYK+C+ A+Q G
Sbjct: 61  WGYTSVQGFRDEMEDDIVIRSDAVDSFSYAAVFDGHAGSSSVKFLREELYKECVGALQAG 120

Query: 121 LLLNGGDFEVIKAALEKAFDDTDKRLLPLLEAAG-KEDESGSTATVAFIRNDVMFISHVG 180
            LLNGGDF  IK AL KAF+  D+ LL  LEA G +EDESGSTATV  IRNDV FI+H+G
Sbjct: 121 SLLNGGDFAAIKEALIKAFESVDRNLLKWLEANGDEEDESGSTATVMIIRNDVSFIAHIG 180

Query: 181 DSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVSRAFGDI 240
           DSC VLSRSG  + LT  HRPYG++  ++QE++R++EAGGWIVNGRICGDI+VSRAFGDI
Sbjct: 181 DSCAVLSRSGQIEELTDYHRPYGSSRAAIQEVKRVKEAGGWIVNGRICGDIAVSRAFGDI 240

Query: 241 RFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNG 300
           RFKTKK                             N ML+KGV+EGRWSEKFVSR++F G
Sbjct: 241 RFKTKK-----------------------------NDMLKKGVDEGRWSEKFVSRIEFKG 300

Query: 301 DLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLAC 353
           D+V A+P++ Q+ L S+VEF+++ASDGLWDYM SS+ V +VR+QL +HG+VQ  C
Sbjct: 301 DMVVATPDIFQVPLTSDVEFIILASDGLWDYMKSSDVVSYVRDQLRKHGNVQATC 325

BLAST of Carg20336-RA vs. TAIR 10
Match: AT1G43900.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 119.4 bits (298), Expect = 7.4e-27
Identity = 101/348 (29.02%), Postives = 162/348 (46.55%), Query Frame = 0

Query: 21  SASKPTNANQLTVRRKTGCCYAIAVDAPSSLTDAAGIR-----WGSTTLQGLREEMEDDA 80
           S+S P   + L +  +       A+ +   ++  AG+R     +G ++L+G R  MED  
Sbjct: 85  SSSSPATKSSLMISSRD----PNALFSGGGISFLAGVRTVKFSYGYSSLKGKRATMEDYF 144

Query: 81  VVRSDGLNGFL--FAAVFDGHGGCSSVKFLREELYKDCITAVQGGLLLNGGDF-EVIKAA 140
             R   +NG +  F  VFDGHGG  + ++L+  L+K+         L++  DF    K A
Sbjct: 145 ETRISDVNGQMVAFFGVFDGHGGARTAEYLKNNLFKN---------LVSHDDFISDTKKA 204

Query: 141 LEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVMFISHVGDSCVVLSRSGGAQVL 200
           + + F  TD+    L+E AG+   +GSTA  AF+  D + +++VGDS VV SR+G A  L
Sbjct: 205 IVEVFKQTDEEY--LIEEAGQPKNAGSTAATAFLIGDKLIVANVGDSRVVASRNGSAVPL 264

Query: 201 TSSHRPYGNNTTSLQEIRRIREAGGWIV---NGRICGDISVSRAFGDIRFKTKKYEFGPF 260
           +  H+P  ++     E +RI +AGG+I+     R+ G ++VSRAFGD + K         
Sbjct: 265 SDDHKPDRSD-----ERQRIEDAGGFIIWAGTWRVGGILAVSRAFGDKQLK--------- 324

Query: 261 NCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVSRVQFNGDLVTASPEVLQL 320
                           PY                                V A PE+ + 
Sbjct: 325 ----------------PY--------------------------------VIAEPEIQEE 350

Query: 321 TLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLACEALAQ 358
            + S +EF+++ASDGLW+ +++ +AV  VR+      D + A   L Q
Sbjct: 385 DI-STLEFIVVASDGLWNVLSNKDAVAIVRD----ISDAETAARKLVQ 350

BLAST of Carg20336-RA vs. TAIR 10
Match: AT3G51470.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 112.8 bits (281), Expect = 6.9e-25
Identity = 91/309 (29.45%), Postives = 132/309 (42.72%), Query Frame = 0

Query: 58  RWGSTTLQGLREEMEDDAVVRSD-----GLNGFLFAAVFDGHGGCSSVKFLREELYKDCI 117
           R GS + +G ++ MED+ +   D     G +   F  VFDGHGG  +  F ++ + K   
Sbjct: 72  RSGSWSDKGPKQSMEDEFICVDDLTEYIGSSTGAFYGVFDGHGGVDAASFTKKNIMK--- 131

Query: 118 TAVQGGLLLNGGDFEV-IKAALEKAFDDTDKRLLPLLEAAGKEDESGSTATVAFIRNDVM 177
                 L++    F    K A   AF  TD     L +A+  +  SG+TA  A I +  M
Sbjct: 132 ------LVMEDKHFPTSTKKATRSAFVKTDH---ALADASSLDRSSGTTALTALILDKTM 191

Query: 178 FISHVGDSCVVLSRSGGAQVLTSSHRPYGNNTTSLQEIRRIREAGGWIVNGRICGDISVS 237
            I++ GDS  VL + G A  L+  H+P   N TS  E  RI + GG I +G + G +SV+
Sbjct: 192 LIANAGDSRAVLGKRGRAIELSKDHKP---NCTS--ERLRIEKLGGVIYDGYLNGQLSVA 251

Query: 238 RAFGDIRFKTKKYEFGPFNCPLIFSSFRRFLFASPYGYILYNRMLRKGVEEGRWSEKFVS 297
           RA GD   K  K    P +C                                        
Sbjct: 252 RALGDWHIKGTKGSLCPLSC---------------------------------------- 311

Query: 298 RVQFNGDLVTASPEVLQLTLGSNVEFVLIASDGLWDYMNSSEAVKFVRNQLSQHGDVQLA 357
                       PE+ ++ L    E++++  DGLWD M+S  AV  VR +L QH D +  
Sbjct: 312 -----------EPELEEIVLTEEDEYLIMGCDGLWDVMSSQCAVTMVRRELMQHNDPERC 312

Query: 358 CEALAQAAL 361
            +AL + AL
Sbjct: 372 SQALVKEAL 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013313.11.4e-253100.00Protein phosphatase 2C 57 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023541443.12.1e-20986.52protein phosphatase 2C 57-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022945263.12.8e-20986.52protein phosphatase 2C 57-like isoform X1 [Cucurbita moschata][more]
KAG6574245.11.4e-20886.52Protein phosphatase 2C 57, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022968153.18.3e-20685.17protein phosphatase 2C 57-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P495999.1e-13155.98Protein phosphatase 2C 57 OS=Arabidopsis thaliana OX=3702 GN=PPH1 PE=1 SV=2[more]
Q5JKN18.3e-10848.42Probable protein phosphatase 2C 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q8VZN91.0e-2529.02Probable protein phosphatase 2C 11 OS=Arabidopsis thaliana OX=3702 GN=At1g43900 ... [more]
P495963.9e-2530.03Probable protein phosphatase 2C T23F11.1 OS=Caenorhabditis elegans OX=6239 GN=pp... [more]
Q8BGL13.3e-2430.10Probable protein phosphatase 1N OS=Mus musculus OX=10090 GN=Ppm1n PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G0B71.3e-20986.52Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114495... [more]
A0A6J1HX684.0e-20685.17Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111467474... [more]
A0A5D3CM488.7e-18577.08Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C2988.7e-18577.08Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103496050 PE=... [more]
A0A0A0L7992.5e-18476.40Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_3G238190 ... [more]
Match NameE-valueIdentityDescription
AT4G27800.16.5e-13255.98thylakoid-associated phosphatase 38 [more]
AT4G27800.22.2e-11659.34thylakoid-associated phosphatase 38 [more]
AT4G27800.35.7e-11258.31thylakoid-associated phosphatase 38 [more]
AT1G43900.17.4e-2729.02Protein phosphatase 2C family protein [more]
AT3G51470.16.9e-2529.45Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 48..401
e-value: 3.5E-71
score: 252.5
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 60..361
e-value: 3.5E-48
score: 164.5
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 58..403
score: 38.43726
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 57..403
e-value: 4.20332E-63
score: 202.557
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 57..409
e-value: 2.8E-80
score: 271.8
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 56..404
NoneNo IPR availablePANTHERPTHR13832:SF589PROTEIN PHOSPHATASE 2C 57coord: 6..440
IPR015655Protein phosphatase 2C familyPANTHERPTHR13832PROTEIN PHOSPHATASE 2Ccoord: 6..440
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 87..95

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg20336Carg20336gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg20336-RA:10Carg20336-RA:10exon
Carg20336-RA:9Carg20336-RA:9exon
Carg20336-RA:8Carg20336-RA:8exon
Carg20336-RA:7Carg20336-RA:7exon
Carg20336-RA:6Carg20336-RA:6exon
Carg20336-RA:5Carg20336-RA:5exon
Carg20336-RA:4Carg20336-RA:4exon
Carg20336-RA:3Carg20336-RA:3exon
Carg20336-RA:2Carg20336-RA:2exon
Carg20336-RA:1Carg20336-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg20336-RA:three_prime_utrCarg20336-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg20336-RA:cdsCarg20336-RA:cds_10CDS
Carg20336-RA:cdsCarg20336-RA:cds_9CDS
Carg20336-RA:cdsCarg20336-RA:cds_8CDS
Carg20336-RA:cdsCarg20336-RA:cds_7CDS
Carg20336-RA:cdsCarg20336-RA:cds_6CDS
Carg20336-RA:cdsCarg20336-RA:cds_5CDS
Carg20336-RA:cdsCarg20336-RA:cds_4CDS
Carg20336-RA:cdsCarg20336-RA:cds_3CDS
Carg20336-RA:cdsCarg20336-RA:cds_2CDS
Carg20336-RA:cdsCarg20336-RA:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg20336-RA:five_prime_utrCarg20336-RA:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg20336-RACarg20336-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0046872 metal ion binding