Carg18549-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18549-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Adenylyl-sulfate kinase
Location	Carg_Chr07: 2903771 .. 2907123 (+)
Sequence length	1070
RNA-Seq Expression	Carg18549-RA
Synteny	Carg18549-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATATATTGCGAAAAGCTGAGTAATTAGTGCTTGATTCCTTGTGTGGATTCCATCTTCTAGTGACAGCTGAGCCTCCCTTCCTCACCCACGCTGTTGTTGCTTTACACATCCGCTTTCTGTCGTCTCTCTGCCTTTTTCAGTTGCCATTCCCATTTATCCGAGCTGAACTACAGGTGGATGTCCCATTGTTTACGGAACTACCTCCGCTACCTCCTCCGACGGACCTCTTTCAACGCTATTTTCAATCTTGTATGTATTTTTGTATCTAATGCTTTCTTGTTTATCTATATTTATGTAGATTTTCGACCGAGGCTTTCGGAGTTTCGAATCTCTTTTTTAGAGTATTATTCTGTGCTTCCTGATGCATTTTTCTGTGTTCAGATCCAAGATGGTCACGTTGCAAGTGGCGCGACCGCTGGTTCACATGAATGAATACCGGCCGTTGATCTCCTCATCTTTGGGGAGGTTGAAGCTGGTGAACGTTGCTGTGAACAATGCGATTGGATTGAAGTGGAGTTGTGGAGGATGTCGGTTCTGTTGCGATGCTGTTGCTACTGGTTTTTTCCAACCATTTAAAGCGTTGACGAACAATTGCTCTGCGATTCACGGTACTTAATTTTTTAAAATCGCGTTTGGTTCTGTTTATGAAGACTAACAAATTCATGTAAATCAAAGAATGTTTAATAGTTTTCATTCCGGATTAATATTTTTGAACCCGCCAATTTGAAGTTGCGGAAATTATATTGGTAAGCAATATCTTGTACTTGAATCAATCAGTCTTAGGTTTAAAACTAAATGCGTTTTGGCTTGAATTTTTTTTATTCCCAGTATTCCCAGTAAGACATGAATTTTTCTTTTCCTTCTGTCCCTATAAAACATCCGATTAATTACCATCTGGATCATTGGTGCCATCAATTAGGTAATTGTTCGTATTCAATAGATGTTAGTTGTGCCTTTGGAGAAAGGTAAAACACAAAATTACTATTTTCGTATCTTTTGTGTAGTTCAAAGGCAAGGCTGATGCATACCAAATTGTTTATTGGTATATTACTTCAAATATAGTTTCTTAGCTTGACTTTACAGGGTCATGACTCATTATTAGCCGAGCTCTAATTTTGTTTACTGAATTTTGAAACTACTTGACTCATCATGACTCATTAGTTTACTGAATTTTGAAACTACTTGGGTACTCTAATTTTGTCCAACCTTTTCATAATTAGGTAAAGGTTGCCATGAGACCTCAACTGGTGGGAAGTCGACAAATATCGTCTGGCATGATTGCCCCGTCGGGAAGCCTCAAAGAGAAAAGTTGATTCAGCAGAAAGGCTGTGTCATATGGATAACTGGTCTCAGTGGTTCAGGTTAGTCCTTGTGTTTTCCAACTGGTAACTGATCTTATTTCTTGTTGAATTTTGAGGACCATCATCACTATCTCCATTATAACTGGTATCATTCCTTCTATGTGATTTTGGCCTTTATTTTGCTCAGAAACTTTTATCTCAAATGCCCGTCACTTAATTTTTTCGCTTTCTATCCTCGTTTGGTTTCTTTTTTTAATCAGAAGTTTGTAAGACTTATTTCACTGGCCAGATTGCAGGATAGATCATCTGAATTTTTTATGATTGCCCGTATGCAGTTTTTAAATAATGATCTTTTTGTATTATGGAGTCTACAAGTCATCTACAAGTTATGTGACTTAGTTGTTCGTTTCATGGAGAAGAGTTCATGAAGCAATACACGATTAAGAGACTAAATTATAGACTAATTGGCTAGACACAGTAGACTTTGTTGTTGTCAACTCTAATCAACTCCGTATAGAGTTAGAGTAGTTTACCATGAAAAGAGGGAAAGTAGACTGAAATAAGGAAATAAAAAATGCAGCTGCTTGTCAAACGATACATGTTTATTGTTTATATTTCAAAAGGTATGAGTAAATTTTTTGGTCATAACTAATTATCATTCTCATGATCAATCATATGAACTTTTCCTGAAAAAGAGATTCCATTATCATCAAACTGGCATTTTACGTTGTACGGTGGTTCTGGTACAATTAAGAGACTGTTGTTTAATATGCTTATGTATAGAATGATCCTTTCACTGTAGTTACTCTCTCCCTCTCTCTCACCTAGGGCTCTAATTATAGGAAAAAGCACTTTGGCATGTGCATTAAGCCAAGGCTTACATTCAAGAAGAAAGCTGACCTACGTCCTTGATGGTGACAATATTAGGCATGGTCTAAACAGTGATCTTAGTTTTGAATCCAAAGATCGAGCGGAAAACATAAGGAGAATTGGTGGGTGGCCTTCTCTATCAGATGAAATATTTCCCTTGACATTTCTTGCATCACCCAGATTTCGTGATATTAATCAAGATGGTCAACAGGTGAGGTAGCAAGCCTATTCGCAGATGCTGGGATTATTTGTATTGCTAGTTTAATATCCCCTTTCCGGAAGGACAGAGATGCCTGTCGAGCCCTGTTGGCAGAAGGCGATTTCATTGAGGTATGTTATACTAATTCTTGTGTTGCATATTATCAGGGCATAGTTTTATTTCCGGTAACTGAGTTCACAACCAGGGCAAGGCATTAGAGATCTGGACATTGATAAGATGGTCATGTCTTCATTGTTGACCGCTGGGTTCTGCAATATTTTTACAGGTTTTCCTTGATGTGCCACTTAAGGTATGTGAAGCCCGGGATCCAAAGGGTCTGTATAAACTGTCCAGAGCAGGAAAGATTCAAGGTACTATTTTGGTTTTCCCCAATATTTGGTTTCACCTGATCCTTCCTCTTAGGATTTGGTAGGTATTCCAAAATTTTCCATCCGTTTCTCTTTTTTGGATGAAACCTTTCTGCCAATCTTGTCTGTAAATATGTGGCTGATTTCTAGGTGGGAATTCAAGTTGCCAATTTTCACGGATGTAGCTGTAGTTGTTGTAATGGAAGATTTATCGTGCTTTGTTTTTGTATACATATATTTCCACAACTGATATCAGTTCTCAATTTAGAGTTGATCTTTGCCATCTATTTTCAGGATTCACGGGGATAGATGATCCATATGAAGAACCATTAAATTGCGAGGTGTGTGTACTTTGAACATACATTTTTGTTTCCTTTTGTTTTCATACGGTCAAAGGATTACATATATTTCAATGCTAACAATTATTTGCCGGCTGTTGAACGAATTGACTACTGTGTTCATCAAACATGAACTAATGAAGAAACATAAATGGACTCACACTGGTCTTTGTTGGCAGATTTTATTGCAATACAAAGAAGGAATCTGCTCGTCCCCTAATGAAATGGCGGAAGAAGTAATTTCATATTTAGAAGAGAGAGGGTACCTGCAAAGT

mRNA sequence

AAATATATTGCGAAAAGCTGAGTAATTAGTGCTTGATTCCTTGTGTGGATTCCATCTTCTAGTGACAGCTGAGCCTCCCTTCCTCACCCACGCTGTTGTTGCTTTACACATCCGCTTTCTGTCGTCTCTCTGCCTTTTTCAGTTGCCATTCCCATTTATCCGAGCTGAACTACAGGTGGATGTCCCATTGTTTACGGAACTACCTCCGCTACCTCCTCCGACGGACCTCTTTCAACGCTATTTTCAATCTTTGGAGTTGTGGAGGATGTCGGTTCTGTTGCGATGCTGTTGCTACTGGTTTTTTCCAACCATTTAAAGCGTTGACGAACAATTGCTCTGCGATTCACGGTAAAGGTTGCCATGAGACCTCAACTGGTGGGAAGTCGACAAATATCGTCTGGCATGATTGCCCCGTCGGGAAGCCTCAAAGAGAAAAGTTGATTCAGCAGAAAGGCTGTGTCATATGGATAACTGGTCTCAGTGGTTCAGGAAAAAGCACTTTGGCATGTGCATTAAGCCAAGGCTTACATTCAAGAAGAAAGCTGACCTACGTCCTTGATGGTGACAATATTAGGCATGGTCTAAACAGTGATCTTAGTTTTGAATCCAAAGATCGAGCGGAAAACATAAGGAGAATTGGTGGGTGGCCTTCTCTATCAGATGAAATATTTCCCTTGACATTTCTTGCATCACCCAGATTTCGTGATATTAATCAAGATGGTCAACAGGTAGCAAGCCTATTCGCAGATGCTGGGATTATTTGTATTGCTAGTTTAATATCCCCTTTCCGGAAGGACAGAGATGCCTGTCGAGCCCTGTTGGCAGAAGGCGATTTCATTGAGGTTTTCCTTGATGTGCCACTTAAGGTATGTGAAGCCCGGGATCCAAAGGGTCTGTATAAACTGTCCAGAGCAGGAAAGATTCAAGGATTCACGGGGATAGATGATCCATATGAAGAACCATTAAATTGCGAGATTTTATTGCAATACAAAGAAGGAATCTGCTCGTCCCCTAATGAAATGGCGGAAGAAGTAATTTCATATTTAGAAGAGAGAGGGTACCTGCAAAGT

Coding sequence (CDS)

ATGTCCCATTGTTTACGGAACTACCTCCGCTACCTCCTCCGACGGACCTCTTTCAACGCTATTTTCAATCTTTGGAGTTGTGGAGGATGTCGGTTCTGTTGCGATGCTGTTGCTACTGGTTTTTTCCAACCATTTAAAGCGTTGACGAACAATTGCTCTGCGATTCACGGTAAAGGTTGCCATGAGACCTCAACTGGTGGGAAGTCGACAAATATCGTCTGGCATGATTGCCCCGTCGGGAAGCCTCAAAGAGAAAAGTTGATTCAGCAGAAAGGCTGTGTCATATGGATAACTGGTCTCAGTGGTTCAGGAAAAAGCACTTTGGCATGTGCATTAAGCCAAGGCTTACATTCAAGAAGAAAGCTGACCTACGTCCTTGATGGTGACAATATTAGGCATGGTCTAAACAGTGATCTTAGTTTTGAATCCAAAGATCGAGCGGAAAACATAAGGAGAATTGGTGGGTGGCCTTCTCTATCAGATGAAATATTTCCCTTGACATTTCTTGCATCACCCAGATTTCGTGATATTAATCAAGATGGTCAACAGGTAGCAAGCCTATTCGCAGATGCTGGGATTATTTGTATTGCTAGTTTAATATCCCCTTTCCGGAAGGACAGAGATGCCTGTCGAGCCCTGTTGGCAGAAGGCGATTTCATTGAGGTTTTCCTTGATGTGCCACTTAAGGTATGTGAAGCCCGGGATCCAAAGGGTCTGTATAAACTGTCCAGAGCAGGAAAGATTCAAGGATTCACGGGGATAGATGATCCATATGAAGAACCATTAAATTGCGAGATTTTATTGCAATACAAAGAAGGAATCTGCTCGTCCCCTAATGAAATGGCGGAAGAAGTAATTTCATATTTAGAAGAGAGAGGGTACCTGCAAAGT

Protein sequence

MSHCLRNYLRYLLRRTSFNAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPYEEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS

Homology

[Close]

BLAST of Carg18549-RA vs. NCBI nr
Match: XP_023517094.1 (adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 502.7 bits (1293), Expect = 2.2e-138
Identity = 247/279 (88.53%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI   WSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLKWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD
Sbjct: 96  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFESKDRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFESKDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS
Sbjct: 276 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 286

[Close]

BLAST of Carg18549-RA vs. NCBI nr
Match: KAG6594977.1 (Adenylyl-sulfate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 502.7 bits (1293), Expect = 2.2e-138
Identity = 247/279 (88.53%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI   WSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 261 NAIGLKWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 320

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD
Sbjct: 321 VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 380

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFESKDRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 381 LSFESKDRAENIRRIG----------------------------EVASLFADAGIICIAS 440

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 441 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 500

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS
Sbjct: 501 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 511

[Close]

BLAST of Carg18549-RA vs. NCBI nr
Match: XP_023003777.1 (adenylyl-sulfate kinase 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 7.1e-137
Identity = 244/279 (87.46%), Postives = 247/279 (88.53%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI   WSCGGCRFCC AVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLKWSCGGCRFCCYAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           +GKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD
Sbjct: 96  IGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFESKDRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFESKDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQ+
Sbjct: 276 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQN 286

[Close]

BLAST of Carg18549-RA vs. NCBI nr
Match: XP_022963439.1 (adenylyl-sulfate kinase 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-134
Identity = 243/279 (87.10%), Postives = 245/279 (87.81%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI    SC GCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLKRSCRGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           V KPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD
Sbjct: 96  VRKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFESKDRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFESKDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQ+
Sbjct: 276 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQN 286

[Close]

BLAST of Carg18549-RA vs. NCBI nr
Match: XP_008440390.1 (PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >KAA0036416.1 adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa] >TYK12812.1 adenylyl-sulfate kinase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 460.7 bits (1184), Expect = 9.7e-126
Identity = 228/279 (81.72%), Postives = 237/279 (84.95%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI    SCGG RFCCDAV T F QPFKA T+ CSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLERSCGGWRFCCDAVPTSFLQPFKAATSICSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLH+RRKLTY+LDGDNIRHGLNSD
Sbjct: 96  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHARRKLTYILDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFE +DRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFEPEDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPL VCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLNVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLN EI+LQYKEGICSSPN+MAE VISYLEERG+LQ+
Sbjct: 276 EEPLNSEIVLQYKEGICSSPNDMAERVISYLEERGHLQA 286

[Close]

BLAST of Carg18549-RA vs. ExPASy Swiss-Prot
Match: O49204 (Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 8.9e-90
Identity = 161/248 (64.92%), Postives = 192/248 (77.42%), Query Frame = 0


Query: 50  NNCSAIHGKGCHETSTGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLA 109
           NNC+   GK   +T+T G STNI+WH C V K +R++ +QQ+GCVIWITGLSGSGKSTLA
Sbjct: 93  NNCNGFPGKKILQTTTVGNSTNILWHKCAVEKSERQEPLQQRGCVIWITGLSGSGKSTLA 152

Query: 110 CALSQGLHSRRKLTYVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFL 169
           CALS+GLH++ KLTY+LDGDN+RHGLNSDLSF+++DRAENIRRIG               
Sbjct: 153 CALSRGLHAKGKLTYILDGDNVRHGLNSDLSFKAEDRAENIRRIG--------------- 212

Query: 170 ASPRFRDINQDGQQVASLFADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLK 229
                        +VA LFADAG+ICIASLISP+RK  DACR+LL EGDFIEVF+DVPLK
Sbjct: 213 -------------EVAKLFADAGVICIASLISPYRKPPDACRSLLPEGDFIEVFMDVPLK 272

Query: 230 VCEARDPKGLYKLSRAGKIQGFTGIDDPYEEPLNCEILLQYKEGICSSPNEMAEEVISYL 289
           VCEARDPKGLYKL+RAGKI+GFTGIDDPYE PL  EI+L  K G+C SP ++A+ VISYL
Sbjct: 273 VCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLKSEIVLHQKLGMCDSPCDLADIVISYL 312

Query: 290 EERGYLQS 298
           EE GYL++
Sbjct: 333 EENGYLKA 312

[Close]

BLAST of Carg18549-RA vs. ExPASy Swiss-Prot
Match: Q43295 (Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1 PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 6.2e-83
Identity = 151/234 (64.53%), Postives = 179/234 (76.50%), Query Frame = 0


Query: 64  STGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLT 123
           ST G STNI WH+C V K  R++L+ QKGCVIW+TGLSGSGKSTLACAL+Q L+ + KL 
Sbjct: 73  STVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQMLYQKGKLC 132

Query: 124 YVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQ 183
           Y+LDGDN+RHGLN DLSF+++DRAENIRR+G                            +
Sbjct: 133 YILDGDNVRHGLNRDLSFKAEDRAENIRRVG----------------------------E 192

Query: 184 VASLFADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLS 243
           VA LFADAGIICIASLISP+R DRDACR+LL EGDF+EVF+DVPL VCEARDPKGLYKL+
Sbjct: 193 VAKLFADAGIICIASLISPYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLA 252

Query: 244 RAGKIQGFTGIDDPYEEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           RAGKI+GFTGIDDPYE PLNCEI L  + G  +SP EMAE+V+ YL+ +GYLQ+
Sbjct: 253 RAGKIKGFTGIDDPYEPPLNCEISLGREGG--TSPIEMAEKVVGYLDNKGYLQA 276

[Close]

BLAST of Carg18549-RA vs. ExPASy Swiss-Prot
Match: Q9SRW7 (Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.4e-82
Identity = 146/234 (62.39%), Postives = 180/234 (76.92%), Query Frame = 0


Query: 64  STGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLT 123
           ST G STNI W + P+GK +R+KL+ QKGCV+WITGLSGSGKSTLAC+LS+ L++R KL+
Sbjct: 2   STVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKLS 61

Query: 124 YVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQ 183
           Y+LDGDN+RHGLN DL F+++DR ENIRR+G                            +
Sbjct: 62  YILDGDNLRHGLNKDLGFKAEDRVENIRRVG----------------------------E 121

Query: 184 VASLFADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLS 243
           VA LFADAG+ICIASLISP+RKDRDACR ++    FIEVF+++ L++CEARDPKGLYKL+
Sbjct: 122 VAKLFADAGLICIASLISPYRKDRDACREMIQNSSFIEVFMNMSLQLCEARDPKGLYKLA 181

Query: 244 RAGKIQGFTGIDDPYEEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           RAGKI+GFTGIDDPYE PLNCEI L+ KEG C SP  MAEEVISYLE++G+LQ+
Sbjct: 182 RAGKIKGFTGIDDPYESPLNCEIELKEKEGECPSPVAMAEEVISYLEDKGFLQN 207

[Close]

BLAST of Carg18549-RA vs. ExPASy Swiss-Prot
Match: Q84JF0 (Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.0e-81
Identity = 155/271 (57.20%), Postives = 191/271 (70.48%), Query Frame = 0


Query: 41  FFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGL 100
           +F+P  A   + S+  G+        GK  NIVWHDCPV K  R++LI+QKGCVIWITGL
Sbjct: 61  YFRPIMAKDESISSRSGE---TKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGL 120

Query: 101 SGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLS 160
           SGSGKS+LACALS+ LH+R KL+Y+LDGDN+RHGLNSDLSFE+ DRAENIRR+G      
Sbjct: 121 SGSGKSSLACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVG------ 180

Query: 161 DEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIASLISPFRKDRDACRALLAEGDFI 220
                                 +VA LFAD+GIICIASLISP+R +R ACRALL +GDFI
Sbjct: 181 ----------------------EVAKLFADSGIICIASLISPYRIERAACRALLPQGDFI 240

Query: 221 EVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPYEEPLNCEILLQYK--EGICSSP 280
           EVF+DVPL VCEARDPKGLYK +RAGKI+GFTG+DDPYE PL+CEI++Q    +G+ SS 
Sbjct: 241 EVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDKGLSSSS 300

Query: 281 N-------------EMAEEVISYLEERGYLQ 297
           +             EMA+ V+SYL++ GYL+
Sbjct: 301 SSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

[Close]

BLAST of Carg18549-RA vs. ExPASy Swiss-Prot
Match: O49196 (Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.3e-77
Identity = 137/230 (59.57%), Postives = 174/230 (75.65%), Query Frame = 0


Query: 68  KSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLD 127
           K+ NIVWH+  + +  R++L+QQKGCV+WITGLSGSGKST+ACALS+ L  R KLTY LD
Sbjct: 91  KAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKALFERGKLTYTLD 150

Query: 128 GDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASL 187
           GDN+RHGLN DL+F+++ R ENIRRIG                            +VA L
Sbjct: 151 GDNVRHGLNRDLTFKAEHRTENIRRIG----------------------------EVAKL 210

Query: 188 FADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGK 247
           FAD G+ICIASLISP+R+DRDACR+LL +GDF+EVF+DVPL VCE+RDPKGLYKL+RAGK
Sbjct: 211 FADVGVICIASLISPYRRDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGK 270

Query: 248 IQGFTGIDDPYEEPLNCEILLQYK-EGICSSPNEMAEEVISYLEERGYLQ 297
           I+GFTGIDDPYE P+NCE++L++  +    SP +MAE +ISYL+ +GYL+
Sbjct: 271 IKGFTGIDDPYEAPVNCEVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

[Close]

BLAST of Carg18549-RA vs. ExPASy TrEMBL
Match: A0A6J1KNK1 (Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111497257 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.4e-137
Identity = 244/279 (87.46%), Postives = 247/279 (88.53%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI   WSCGGCRFCC AVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLKWSCGGCRFCCYAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           +GKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD
Sbjct: 96  IGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFESKDRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFESKDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQ+
Sbjct: 276 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQN 286

[Close]

BLAST of Carg18549-RA vs. ExPASy TrEMBL
Match: A0A6J1HK41 (Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111463644 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.2e-135
Identity = 243/279 (87.10%), Postives = 245/279 (87.81%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI    SC GCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLKRSCRGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           V KPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD
Sbjct: 96  VRKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFESKDRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFESKDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQ+
Sbjct: 276 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQN 286

[Close]

BLAST of Carg18549-RA vs. ExPASy TrEMBL
Match: A0A5D3CN90 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004040 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.7e-126
Identity = 228/279 (81.72%), Postives = 237/279 (84.95%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI    SCGG RFCCDAV T F QPFKA T+ CSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLERSCGGWRFCCDAVPTSFLQPFKAATSICSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLH+RRKLTY+LDGDNIRHGLNSD
Sbjct: 96  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHARRKLTYILDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFE +DRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFEPEDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPL VCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLNVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLN EI+LQYKEGICSSPN+MAE VISYLEERG+LQ+
Sbjct: 276 EEPLNSEIVLQYKEGICSSPNDMAERVISYLEERGHLQA 286

[Close]

BLAST of Carg18549-RA vs. ExPASy TrEMBL
Match: A0A1S3B0L2 (Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103484852 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.7e-126
Identity = 228/279 (81.72%), Postives = 237/279 (84.95%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI    SCGG RFCCDAV T F QPFKA T+ CSAIHGKGCHETSTGGKSTNIVWHDCP
Sbjct: 36  NAIGLERSCGGWRFCCDAVPTSFLQPFKAATSICSAIHGKGCHETSTGGKSTNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLH+RRKLTY+LDGDNIRHGLNSD
Sbjct: 96  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHARRKLTYILDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFE +DRAENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFEPEDRAENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPL VCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLNVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLN EI+LQYKEGICSSPN+MAE VISYLEERG+LQ+
Sbjct: 276 EEPLNSEIVLQYKEGICSSPNDMAERVISYLEERGHLQA 286

[Close]

BLAST of Carg18549-RA vs. ExPASy TrEMBL
Match: A0A0A0KFY6 (Adenylyl-sulfate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G495640 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.2e-124
Identity = 225/279 (80.65%), Postives = 235/279 (84.23%), Query Frame = 0


Query: 19  NAIFNLWSCGGCRFCCDAVATGFFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCP 78
           NAI    SCGG RFCCDAV T F QPFKA T+ CSAIHGKGCHETSTGGKS NIVWHDCP
Sbjct: 36  NAIGLERSCGGWRFCCDAVPTSFLQPFKAATSICSAIHGKGCHETSTGGKSKNIVWHDCP 95

Query: 79  VGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSD 138
           VGKPQREKL+QQKGCVIWITGLSGSGKSTLACALSQGLH+RRKLTY+LDGDNIRHGLNSD
Sbjct: 96  VGKPQREKLLQQKGCVIWITGLSGSGKSTLACALSQGLHARRKLTYILDGDNIRHGLNSD 155

Query: 139 LSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIAS 198
           LSFE +DR ENIRRIG                            +VASLFADAGIICIAS
Sbjct: 156 LSFEPEDRTENIRRIG----------------------------EVASLFADAGIICIAS 215

Query: 199 LISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPY 258
           LISPFRKDRDACRALLAEGDFIEVFLDVPL VCEARDPKGLYKLSRAGKIQGFTGIDDPY
Sbjct: 216 LISPFRKDRDACRALLAEGDFIEVFLDVPLNVCEARDPKGLYKLSRAGKIQGFTGIDDPY 275

Query: 259 EEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           EEPLN EI+LQYKEGICSSPN+MAE VISYLEERG+LQ+
Sbjct: 276 EEPLNSEIVLQYKEGICSSPNDMAERVISYLEERGHLQT 286

[Close]

BLAST of Carg18549-RA vs. TAIR 10
Match: AT2G14750.1 (APS kinase )

HSP 1 Score: 308.9 bits (790), Expect = 4.4e-84
Identity = 151/234 (64.53%), Postives = 179/234 (76.50%), Query Frame = 0


Query: 64  STGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLT 123
           ST G STNI WH+C V K  R++L+ QKGCVIW+TGLSGSGKSTLACAL+Q L+ + KL 
Sbjct: 73  STVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQMLYQKGKLC 132

Query: 124 YVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQ 183
           Y+LDGDN+RHGLN DLSF+++DRAENIRR+G                            +
Sbjct: 133 YILDGDNVRHGLNRDLSFKAEDRAENIRRVG----------------------------E 192

Query: 184 VASLFADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLS 243
           VA LFADAGIICIASLISP+R DRDACR+LL EGDF+EVF+DVPL VCEARDPKGLYKL+
Sbjct: 193 VAKLFADAGIICIASLISPYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLA 252

Query: 244 RAGKIQGFTGIDDPYEEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           RAGKI+GFTGIDDPYE PLNCEI L  + G  +SP EMAE+V+ YL+ +GYLQ+
Sbjct: 253 RAGKIKGFTGIDDPYEPPLNCEISLGREGG--TSPIEMAEKVVGYLDNKGYLQA 276

[Close]

BLAST of Carg18549-RA vs. TAIR 10
Match: AT3G03900.1 (adenosine-5'-phosphosulfate (APS) kinase 3 )

HSP 1 Score: 307.8 bits (787), Expect = 9.7e-84
Identity = 146/234 (62.39%), Postives = 180/234 (76.92%), Query Frame = 0


Query: 64  STGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLT 123
           ST G STNI W + P+GK +R+KL+ QKGCV+WITGLSGSGKSTLAC+LS+ L++R KL+
Sbjct: 2   STVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKLS 61

Query: 124 YVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQ 183
           Y+LDGDN+RHGLN DL F+++DR ENIRR+G                            +
Sbjct: 62  YILDGDNLRHGLNKDLGFKAEDRVENIRRVG----------------------------E 121

Query: 184 VASLFADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLS 243
           VA LFADAG+ICIASLISP+RKDRDACR ++    FIEVF+++ L++CEARDPKGLYKL+
Sbjct: 122 VAKLFADAGLICIASLISPYRKDRDACREMIQNSSFIEVFMNMSLQLCEARDPKGLYKLA 181

Query: 244 RAGKIQGFTGIDDPYEEPLNCEILLQYKEGICSSPNEMAEEVISYLEERGYLQS 298
           RAGKI+GFTGIDDPYE PLNCEI L+ KEG C SP  MAEEVISYLE++G+LQ+
Sbjct: 182 RAGKIKGFTGIDDPYESPLNCEIELKEKEGECPSPVAMAEEVISYLEDKGFLQN 207

[Close]

BLAST of Carg18549-RA vs. TAIR 10
Match: AT5G67520.1 (adenosine-5'-phosphosulfate (APS) kinase 4 )

HSP 1 Score: 303.9 bits (777), Expect = 1.4e-82
Identity = 155/271 (57.20%), Postives = 191/271 (70.48%), Query Frame = 0


Query: 41  FFQPFKALTNNCSAIHGKGCHETSTGGKSTNIVWHDCPVGKPQREKLIQQKGCVIWITGL 100
           +F+P  A   + S+  G+        GK  NIVWHDCPV K  R++LI+QKGCVIWITGL
Sbjct: 61  YFRPIMAKDESISSRSGE---TKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGL 120

Query: 101 SGSGKSTLACALSQGLHSRRKLTYVLDGDNIRHGLNSDLSFESKDRAENIRRIGGWPSLS 160
           SGSGKS+LACALS+ LH+R KL+Y+LDGDN+RHGLNSDLSFE+ DRAENIRR+G      
Sbjct: 121 SGSGKSSLACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVG------ 180

Query: 161 DEIFPLTFLASPRFRDINQDGQQVASLFADAGIICIASLISPFRKDRDACRALLAEGDFI 220
                                 +VA LFAD+GIICIASLISP+R +R ACRALL +GDFI
Sbjct: 181 ----------------------EVAKLFADSGIICIASLISPYRIERAACRALLPQGDFI 240

Query: 221 EVFLDVPLKVCEARDPKGLYKLSRAGKIQGFTGIDDPYEEPLNCEILLQYK--EGICSSP 280
           EVF+DVPL VCEARDPKGLYK +RAGKI+GFTG+DDPYE PL+CEI++Q    +G+ SS 
Sbjct: 241 EVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRDKGLSSSS 300

Query: 281 N-------------EMAEEVISYLEERGYLQ 297
           +             EMA+ V+SYL++ GYL+
Sbjct: 301 SSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

[Close]

BLAST of Carg18549-RA vs. TAIR 10
Match: AT4G39940.1 (APS-kinase 2 )

HSP 1 Score: 291.2 bits (744), Expect = 9.4e-79
Identity = 137/230 (59.57%), Postives = 174/230 (75.65%), Query Frame = 0


Query: 68  KSTNIVWHDCPVGKPQREKLIQQKGCVIWITGLSGSGKSTLACALSQGLHSRRKLTYVLD 127
           K+ NIVWH+  + +  R++L+QQKGCV+WITGLSGSGKST+ACALS+ L  R KLTY LD
Sbjct: 91  KAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKALFERGKLTYTLD 150

Query: 128 GDNIRHGLNSDLSFESKDRAENIRRIGGWPSLSDEIFPLTFLASPRFRDINQDGQQVASL 187
           GDN+RHGLN DL+F+++ R ENIRRIG                            +VA L
Sbjct: 151 GDNVRHGLNRDLTFKAEHRTENIRRIG----------------------------EVAKL 210

Query: 188 FADAGIICIASLISPFRKDRDACRALLAEGDFIEVFLDVPLKVCEARDPKGLYKLSRAGK 247
           FAD G+ICIASLISP+R+DRDACR+LL +GDF+EVF+DVPL VCE+RDPKGLYKL+RAGK
Sbjct: 211 FADVGVICIASLISPYRRDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGK 270

Query: 248 IQGFTGIDDPYEEPLNCEILLQYK-EGICSSPNEMAEEVISYLEERGYLQ 297
           I+GFTGIDDPYE P+NCE++L++  +    SP +MAE +ISYL+ +GYL+
Sbjct: 271 IKGFTGIDDPYEAPVNCEVVLKHTGDDESCSPRQMAENIISYLQNKGYLE 292

The following BLAST results are available for this feature:

BLAST of Carg18549-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023517094.1	2.2e-138	88.53	adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo]	[more]
KAG6594977.1	2.2e-138	88.53	Adenylyl-sulfate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023003777.1	7.1e-137	87.46	adenylyl-sulfate kinase 3-like isoform X1 [Cucurbita maxima]	[more]
XP_022963439.1	1.5e-134	87.10	adenylyl-sulfate kinase 3-like isoform X1 [Cucurbita moschata]	[more]
XP_008440390.1	9.7e-126	81.72	PREDICTED: adenylyl-sulfate kinase 3 [Cucumis melo] >KAA0036416.1 adenylyl-sulfa...	[more]

BLAST of Carg18549-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49204	8.9e-90	64.92	Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=...	[more]
Q43295	6.2e-83	64.53	Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1...	[more]
Q9SRW7	1.4e-82	62.39	Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1	[more]
Q84JF0	2.0e-81	57.20	Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4...	[more]
O49196	1.3e-77	59.57	Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2...	[more]

BLAST of Carg18549-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KNK1	3.4e-137	87.46	Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111497257 PE=3 SV=1	[more]
A0A6J1HK41	7.2e-135	87.10	Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111463644 PE=3 SV=1	[more]
A0A5D3CN90	4.7e-126	81.72	Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3B0L2	4.7e-126	81.72	Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103484852 PE=3 SV=1	[more]
A0A0A0KFY6	1.2e-124	80.65	Adenylyl-sulfate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G495640 PE=3 SV=1	[more]

BLAST of Carg18549-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14750.1	4.4e-84	64.53	APS kinase	[more]
AT3G03900.1	9.7e-84	62.39	adenosine-5'-phosphosulfate (APS) kinase 3	[more]
AT5G67520.1	1.4e-82	57.20	adenosine-5'-phosphosulfate (APS) kinase 4	[more]
AT4G39940.1	9.4e-79	59.57	APS-kinase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 68..297 e-value: 4.4E-83 score: 279.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 90..292
None	No IPR available	PFAM	PF01583	APS_kinase	coord: 91..154 e-value: 1.8E-25 score: 89.7 coord: 172..271 e-value: 2.3E-35 score: 121.8
None	No IPR available	PANTHER	PTHR11055	BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE	coord: 47..155
None	No IPR available	PANTHER	PTHR11055	BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE	coord: 166..297
None	No IPR available	PANTHER	PTHR11055:SF55	ADENYLYL-SULFATE KINASE 1, CHLOROPLASTIC	coord: 166..297 coord: 47..155
IPR002891	Adenylyl-sulfate kinase	CDD	cd02027	APSK	coord: 94..268 e-value: 6.57299E-89 score: 259.718

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg18549	Carg18549	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg18549-RA:1	Carg18549-RA:1	exon
Carg18549-RA:2	Carg18549-RA:2	exon
Carg18549-RA:3	Carg18549-RA:3	exon
Carg18549-RA:4	Carg18549-RA:4	exon
Carg18549-RA:5	Carg18549-RA:5	exon
Carg18549-RA:6	Carg18549-RA:6	exon
Carg18549-RA:7	Carg18549-RA:7	exon
Carg18549-RA:8	Carg18549-RA:8	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg18549-RA:five_prime_utr	Carg18549-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg18549-RA:cds	Carg18549-RA:cds	CDS
Carg18549-RA:cds	Carg18549-RA:cds_2	CDS
Carg18549-RA:cds	Carg18549-RA:cds_3	CDS
Carg18549-RA:cds	Carg18549-RA:cds_4	CDS
Carg18549-RA:cds	Carg18549-RA:cds_5	CDS
Carg18549-RA:cds	Carg18549-RA:cds_6	CDS
Carg18549-RA:cds	Carg18549-RA:cds_7	CDS
Carg18549-RA:cds	Carg18549-RA:cds_8	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg18549-RA	Carg18549-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000103	sulfate assimilation
biological_process	GO:0070814	hydrogen sulfide biosynthetic process
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0004020	adenylylsulfate kinase activity
molecular_function	GO:0005524	ATP binding