Carg18523-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18523-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	1-aminocyclopropane-1-carboxylate synthase
Location	Carg_Chr07: 2791167 .. 2792897 (-)
Sequence length	1428
RNA-Seq Expression	Carg18523-RA
Synteny	Carg18523-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATGCTATCCACCAAGGCCACATGCAACTCCCACGGTCAAGATTCCTCCTACTTCTTGGGATGGGAGGCTTATGAAAACAATCCCTTCCATCACACTTCTAATCCCAACGGCATAATTCAAATGGGTCTCGCTGAAAATCAGGTATCTATCTAATTATTATTCTCCATTTCCCCTTTTGCAACTTCAATCCCTCACTCACCTTTTTCTTCATCTCCTAATTTTTCAGCTATCCTTTGATCTTCTTGAATCATGGCTCTCTAAGAATCCAGACGCCGCCAGCTTCAAACGTGATGGCAAATCCATTTTCAGAGAGCTCGCCCTCTTCCAAGATTACCATGGCTTACCCGCATTCAAAAAGGTATACATATTTATTCATACATACGATTTATAATTGAATTCTACAATATACTTAGAATTATAAATTAATTAAGGTTTTTTTTTTTTTTTTCTTTTCAATTTCAGGCATTGGTTGAATTTATGGCGGAAATTAGAGGAAACAAAGTATCATTTGAGGCTAATAACATAGTCCTCACAGCTGGAGCCACGTCAGCAAATGAGACTCTTATGTTCTGCCTTGCTGAAGCTGGCGACGCTTTTCTCCTCCCAACACCATACTATCCAGGGTATGTTTATATCTTTACATTCACTTAAATAATAAGCTTTACCAAAAATTTAAGCTATATGCATTAATTAATTACAATAATTCTCATCTTTACTTCCACTTGTTATTCAAACAGATTTGATAGAGATCTCAAATGGAGAACCGGCGTTGAGATTGTGCCAATTCATTGCACTAGCTCCAATGCCTTCCAAATCACCCAACGCGCTCTTGAACAAGCCTACAAAGACGCCCAATCTCGCAACCTACGCGTCAAAGGCGTGTTGGTCACAAACCCATCTAACCCATTAGGCACCACAATGAGCCGGGATGAACTCAACCTTGTCTTTGATTTCATAACCTCCAAAGGAATTCATTTGATCAGCGATGAGATCTACTCCGGCACCGTGTTTGGGTCCTCGGGATTTGTGAGTGCGATGGAAGTGCTCAAGGAGAGGAGTAGCCAAGACGAGGAGGTTTGGAAGAGAGTGCATATTGTTTACAGTTTGTCTAAAGATTTGGGTCTTCCAGGGTTTCGCGTTGGTGCAATTTACTCAAATGATGACATGGTGGTGGCTGCCGCTACCAAAATGTCCAGCTTTGGGTTGGTCTCATCTCAAACACAATACCTTCTCTCCGCTATGCTCTCCGACAAGAAATTCACCACAAACTACATTACCGAGAATCAAAAGAGGCTAAAACAAAGACAAAAAATGCTCGTGTCCGGCTTACAAAAGGCCGGTATTAACTGCCTGGATAGCAACGCCGGGCTGTTTTGTTGGGTGGACATGAGGCATTTGTTGGAATCCGACACGTTTGAATCCGAGCTAGAGCTGTGGAAGAAGATTGTGTACGAGGTGGGTTTGAACATTTCGCCTGGATCATCATGTCATTGCACTGAACCAGGGTGGTTCCGTGTTTGCTTTGCTAATATGTCGGAATCAACATTGAAACTCGCCGTCCGAAGATTGAAGTCGTTTGTGACAGAATTGACGTCCATTACTACTTCAAATCATCGTAATCATCACAATAAAATTTGCAAAAACATCAAGAAAAACATCTTCACCAAATGGGTTTTCCGGCAATCAGTTCAAGAAGCCAACCGCAAGATGCAAGCTGAACGATAG

mRNA sequence

ATGAAGATGCTATCCACCAAGGCCACATGCAACTCCCACGGTCAAGATTCCTCCTACTTCTTGGGATGGGAGGCTTATGAAAACAATCCCTTCCATCACACTTCTAATCCCAACGGCATAATTCAAATGGGTCTCGCTGAAAATCAGCTATCCTTTGATCTTCTTGAATCATGGCTCTCTAAGAATCCAGACGCCGCCAGCTTCAAACGTGATGGCAAATCCATTTTCAGAGAGCTCGCCCTCTTCCAAGATTACCATGGCTTACCCGCATTCAAAAAGGCATTGGTTGAATTTATGGCGGAAATTAGAGGAAACAAAGTATCATTTGAGGCTAATAACATAGTCCTCACAGCTGGAGCCACGTCAGCAAATGAGACTCTTATGTTCTGCCTTGCTGAAGCTGGCGACGCTTTTCTCCTCCCAACACCATACTATCCAGGATTTGATAGAGATCTCAAATGGAGAACCGGCGTTGAGATTGTGCCAATTCATTGCACTAGCTCCAATGCCTTCCAAATCACCCAACGCGCTCTTGAACAAGCCTACAAAGACGCCCAATCTCGCAACCTACGCGTCAAAGGCGTGTTGGTCACAAACCCATCTAACCCATTAGGCACCACAATGAGCCGGGATGAACTCAACCTTGTCTTTGATTTCATAACCTCCAAAGGAATTCATTTGATCAGCGATGAGATCTACTCCGGCACCGTGTTTGGGTCCTCGGGATTTGTGAGTGCGATGGAAGTGCTCAAGGAGAGGAGTAGCCAAGACGAGGAGGTTTGGAAGAGAGTGCATATTGTTTACAGTTTGTCTAAAGATTTGGGTCTTCCAGGGTTTCGCGTTGGTGCAATTTACTCAAATGATGACATGGTGGTGGCTGCCGCTACCAAAATGTCCAGCTTTGGGTTGGTCTCATCTCAAACACAATACCTTCTCTCCGCTATGCTCTCCGACAAGAAATTCACCACAAACTACATTACCGAGAATCAAAAGAGGCTAAAACAAAGACAAAAAATGCTCGTGTCCGGCTTACAAAAGGCCGGTATTAACTGCCTGGATAGCAACGCCGGGCTGTTTTGTTGGGTGGACATGAGGCATTTGTTGGAATCCGACACGTTTGAATCCGAGCTAGAGCTGTGGAAGAAGATTGTGTACGAGGTGGGTTTGAACATTTCGCCTGGATCATCATGTCATTGCACTGAACCAGGGTGGTTCCGTGTTTGCTTTGCTAATATGTCGGAATCAACATTGAAACTCGCCGTCCGAAGATTGAAGTCGTTTGTGACAGAATTGACGTCCATTACTACTTCAAATCATCGTAATCATCACAATAAAATTTGCAAAAACATCAAGAAAAACATCTTCACCAAATGGGTTTTCCGGCAATCAGTTCAAGAAGCCAACCGCAAGATGCAAGCTGAACGATAG

Coding sequence (CDS)

ATGAAGATGCTATCCACCAAGGCCACATGCAACTCCCACGGTCAAGATTCCTCCTACTTCTTGGGATGGGAGGCTTATGAAAACAATCCCTTCCATCACACTTCTAATCCCAACGGCATAATTCAAATGGGTCTCGCTGAAAATCAGCTATCCTTTGATCTTCTTGAATCATGGCTCTCTAAGAATCCAGACGCCGCCAGCTTCAAACGTGATGGCAAATCCATTTTCAGAGAGCTCGCCCTCTTCCAAGATTACCATGGCTTACCCGCATTCAAAAAGGCATTGGTTGAATTTATGGCGGAAATTAGAGGAAACAAAGTATCATTTGAGGCTAATAACATAGTCCTCACAGCTGGAGCCACGTCAGCAAATGAGACTCTTATGTTCTGCCTTGCTGAAGCTGGCGACGCTTTTCTCCTCCCAACACCATACTATCCAGGATTTGATAGAGATCTCAAATGGAGAACCGGCGTTGAGATTGTGCCAATTCATTGCACTAGCTCCAATGCCTTCCAAATCACCCAACGCGCTCTTGAACAAGCCTACAAAGACGCCCAATCTCGCAACCTACGCGTCAAAGGCGTGTTGGTCACAAACCCATCTAACCCATTAGGCACCACAATGAGCCGGGATGAACTCAACCTTGTCTTTGATTTCATAACCTCCAAAGGAATTCATTTGATCAGCGATGAGATCTACTCCGGCACCGTGTTTGGGTCCTCGGGATTTGTGAGTGCGATGGAAGTGCTCAAGGAGAGGAGTAGCCAAGACGAGGAGGTTTGGAAGAGAGTGCATATTGTTTACAGTTTGTCTAAAGATTTGGGTCTTCCAGGGTTTCGCGTTGGTGCAATTTACTCAAATGATGACATGGTGGTGGCTGCCGCTACCAAAATGTCCAGCTTTGGGTTGGTCTCATCTCAAACACAATACCTTCTCTCCGCTATGCTCTCCGACAAGAAATTCACCACAAACTACATTACCGAGAATCAAAAGAGGCTAAAACAAAGACAAAAAATGCTCGTGTCCGGCTTACAAAAGGCCGGTATTAACTGCCTGGATAGCAACGCCGGGCTGTTTTGTTGGGTGGACATGAGGCATTTGTTGGAATCCGACACGTTTGAATCCGAGCTAGAGCTGTGGAAGAAGATTGTGTACGAGGTGGGTTTGAACATTTCGCCTGGATCATCATGTCATTGCACTGAACCAGGGTGGTTCCGTGTTTGCTTTGCTAATATGTCGGAATCAACATTGAAACTCGCCGTCCGAAGATTGAAGTCGTTTGTGACAGAATTGACGTCCATTACTACTTCAAATCATCGTAATCATCACAATAAAATTTGCAAAAACATCAAGAAAAACATCTTCACCAAATGGGTTTTCCGGCAATCAGTTCAAGAAGCCAACCGCAAGATGCAAGCTGAACGATAG

Protein sequence

MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLSKNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGATSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQAYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGSSGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSSFGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFCWVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLAVRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER

Homology

[Close]

BLAST of Carg18523-RA vs. NCBI nr
Match: KAG6594949.1 (1-aminocyclopropane-1-carboxylate synthase 101, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026910.1 1-aminocyclopropane-1-carboxylate synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 955.3 bits (2468), Expect = 2.0e-274
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER
Sbjct: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 475

[Close]

BLAST of Carg18523-RA vs. NCBI nr
Match: XP_022963198.1 (1-aminocyclopropane-1-carboxylate synthase CMA101 [Cucurbita moschata])

HSP 1 Score: 951.8 bits (2459), Expect = 2.2e-273
Identity = 474/475 (99.79%), Postives = 474/475 (99.79%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           VRRLKSFVTELTSITTSNHRNH NKICKNIKKNIFTKWVFRQSVQEANRKMQAER
Sbjct: 421 VRRLKSFVTELTSITTSNHRNHDNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 475

[Close]

BLAST of Carg18523-RA vs. NCBI nr
Match: XP_023518664.1 (1-aminocyclopropane-1-carboxylate synthase CMA101 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 949.1 bits (2452), Expect = 1.4e-272
Identity = 472/475 (99.37%), Postives = 473/475 (99.58%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           SGFVSAMEVLKERSSQDEEVWKR HIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 SGFVSAMEVLKERSSQDEEVWKRAHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESEL+LWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELQLWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           VRRLKSFVTELTSITTSNHRNH NKICKNIKKNIFTKWVFRQSVQEANRKMQAER
Sbjct: 421 VRRLKSFVTELTSITTSNHRNHDNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 475

[Close]

BLAST of Carg18523-RA vs. NCBI nr
Match: XP_023003465.1 (1-aminocyclopropane-1-carboxylate synthase CMA101 [Cucurbita maxima] >Q00257.1 RecName: Full=1-aminocyclopropane-1-carboxylate synthase CMA101; Short=ACC synthase; AltName: Full=S-adenosyl-L-methionine methylthioadenosine-lyase [Cucurbita maxima] >AAA91152.1 1-aminocyclopropane-1-carboxylic acid synthase [Cucurbita maxima] >BAA00839.1 1-aminocyclopropane-1-carboxylate synthase [Cucurbita maxima] >prf||1916421B aminocyclopropane carboxylate synthase:ISOTYPE=auxin-induced [Cucurbita maxima])

HSP 1 Score: 928.7 bits (2399), Expect = 2.0e-266
Identity = 461/475 (97.05%), Postives = 466/475 (98.11%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQITQ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKDAQ+RNLRVKGVLVTNPSNPLGTTM+RDELNLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVSAMEVLKERSS+DEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT +YI+ENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESD FESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           VRRLKSFVTEL S TTSNHRNH NKICKNIKKNIFTKWVFRQS QEANRKMQAER
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKKNIFTKWVFRQSAQEANRKMQAER 475

[Close]

BLAST of Carg18523-RA vs. NCBI nr
Match: XP_023544912.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.1 bits (2265), Expect = 6.9e-251
Identity = 436/478 (91.21%), Postives = 456/478 (95.40%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           M+MLSTKATCNSHGQDSSYFLGWEAYE NPF   SNP GIIQMGLAENQLSFDLLESWL+
Sbjct: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDG+SIFRELALFQDYHGLPAFKKALVEFMAEIRGNKV+FEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQ+TQ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYK+A++RNLRVKGVLVTNPSNPLGTTMSR EL+LVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVSAMEVL+ERSS++EEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT NYITENQ+RLKQRQKMLVSGL+KAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHC+EPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHR---NHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           +RRLKSFV EL S  + N +   N+ NK CKNIKKNIFTKWVFR SVQEANRK+Q ER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478

[Close]

BLAST of Carg18523-RA vs. ExPASy Swiss-Prot
Match: Q00257 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN=ACS2 PE=2 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 2.6e-269
Identity = 461/475 (97.05%), Postives = 466/475 (98.11%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQITQ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKDAQ+RNLRVKGVLVTNPSNPLGTTM+RDELNLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVSAMEVLKERSS+DEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT +YI+ENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESD FESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           VRRLKSFVTEL S TTSNHRNH NKICKNIKKNIFTKWVFRQS QEANRKMQAER
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKKNIFTKWVFRQSAQEANRKMQAER 475

[Close]

BLAST of Carg18523-RA vs. ExPASy Swiss-Prot
Match: Q42881 (1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=ACS3 PE=1 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.1e-211
Identity = 358/470 (76.17%), Postives = 406/470 (86.38%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MK+LS KATCNSHGQDSSYFLGW+ YE NP+    NP GIIQMGLAENQLSFDLLESWL+
Sbjct: 1   MKLLSEKATCNSHGQDSSYFLGWQEYEKNPYDEIQNPKGIIQMGLAENQLSFDLLESWLA 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           +NPDAA FKR+G+SIFRELALFQDYHGLPAFK A+ +FM+EIRGN+VSF++NN+VLTAGA
Sbjct: 61  QNPDAAGFKRNGESIFRELALFQDYHGLPAFKNAMTKFMSEIRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLA  GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN F+IT+ ALE+
Sbjct: 121 TSANETLMFCLANQGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFRITESALEE 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY DA+ RNL+VKGVLVTNPSNPLGTT++R+EL L+  FI  KGIHLISDEIYSGTVF S
Sbjct: 181 AYLDAKKRNLKVKGVLVTNPSNPLGTTLNRNELELLLTFIDEKGIHLISDEIYSGTVFNS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVS MEVL E++     VW+RVHIVYSLSKDLGLPGFR+GAIYSND+MVV+AATKMSS
Sbjct: 241 PGFVSVMEVLIEKNYMKTRVWERVHIVYSLSKDLGLPGFRIGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLS MLSDKKFT  YI+ENQKRLK+R  MLV GL+ AGINCL+SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSCMLSDKKFTKKYISENQKRLKKRHAMLVKGLKSAGINCLESNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL S+ F++E++LWKKIVY+VGLNISPGSSCHCTEPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLSSNNFDAEMDLWKKIVYDVGLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRK 471
           +RR+K FV E T+   +NH+N       N KK  F+KWVFR S  +  R+
Sbjct: 421 MRRIKDFV-ESTAPNATNHQNQQQSNA-NSKKKSFSKWVFRLSFNDRQRE 468

[Close]

BLAST of Carg18523-RA vs. ExPASy Swiss-Prot
Match: P37821 (1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 PE=1 SV=2)

HSP 1 Score: 717.6 bits (1851), Expect = 9.2e-206
Identity = 349/467 (74.73%), Postives = 406/467 (86.94%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           M+MLS  AT NSHGQDSSYFLGW+ YE NP+H   N NGIIQMGLAENQL FDLLESWL+
Sbjct: 1   MRMLSRNATFNSHGQDSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLA 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNP+AA+FK++G+SIF ELALFQDYHGLPAFKKA+V+FMAEIRGNKV+F+ N++VLTAGA
Sbjct: 61  KNPEAAAFKKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANET +FCLA+ G+A L+PTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQIT+ ALE+
Sbjct: 121 TSANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEE 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY++A+ RNLRVKGVLVTNPSNPLGTTM+R+EL L+  F+  KGIHLISDEIYSGT F S
Sbjct: 181 AYQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSS 240

Query: 241 SGFVSAMEVLKERS-SQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300
             F+S MEVLK+R+  ++ EVW+RVH+VYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS
Sbjct: 241 PSFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLF 360
           SFGLVSSQTQ+LLSAMLSDKK T NYI EN KRLKQRQK LVSGLQK+GI+CL+ NAGLF
Sbjct: 301 SFGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGLF 360

Query: 361 CWVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKL 420
           CWVDMRHLL S+TFE+E+ELWKKIVYEV LNISPGSSCHCTEPGWFRVCFAN+ E TL L
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCHCTEPGWFRVCFANLPERTLDL 420

Query: 421 AVRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQE 467
           A++RLK+FV E  ++   N  +  + +  + ++++ TKWV R S  +
Sbjct: 421 AMQRLKAFVGEYYNVPEVNGGSQSSHLSHSRRQSL-TKWVSRLSFDD 466

[Close]

BLAST of Carg18523-RA vs. ExPASy Swiss-Prot
Match: Q37001 (1-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=ACS5 PE=1 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 6.6e-204
Identity = 346/463 (74.73%), Postives = 397/463 (85.75%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MK LSTK T N HGQDSSYFLGWE YE NP+    NPNG+IQMGLAENQL FDL+ESWL+
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAAS KR+G+SIFRELALFQDYHG+P FKKA+ EFM EIRGN+V+F+   IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQIT+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY+ AQ  +L+VKGVLVTNPSNPLGT ++R ELNL+ DFITSK IHLISDEIYSGT+FG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             F+S M+VLK++  +D EV KRVH+VYSLSKDLGLPGFRVGAIYSND+M+V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT+ Y+ ENQKRLK RQ+ LVSGL+ AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+EL+LWKKIVY V LNISPGSSCHCTEPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQS 464
           ++RLK+FV E T       R+ H ++ K+++K   + WVFR S
Sbjct: 421 LKRLKTFV-ESTDCGRMISRSSHERL-KSLRKKTVSNWVFRVS 461

[Close]

BLAST of Carg18523-RA vs. ExPASy Swiss-Prot
Match: Q9M2Y8 (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=ACS9 PE=1 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 1.5e-203
Identity = 349/463 (75.38%), Postives = 399/463 (86.18%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MK LS K T N+HGQDSSYFLGWE YE NP+    NPNGIIQMGLAENQL FDL+E+WL+
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAA  K+DG+SIF+ELALFQDYHGLP FKKAL EFM EIRGN+V+F+ + IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQIT+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY+ AQ  +L+VKGVLVTNPSNPLGT ++R ELNL+ DFITSK IHLISDEIYSGTVFG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             FVS M+VLK+++ ++ EV KRVHIVYSLSKDLGLPGFRVGAIYSND+MVV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT+ Y+ ENQKRLK RQK LVSGL+ AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+ELELWKKIVY+V LNISPGSSCHCTEPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQS 464
           ++RLK +V E T       ++ H++I K+++K   + WVFR S
Sbjct: 421 MKRLKEYV-ESTDSRRVISKSSHDRI-KSLRKRTVSNWVFRVS 461

[Close]

BLAST of Carg18523-RA vs. ExPASy TrEMBL
Match: A0A6J1HH23 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita moschata OX=3662 GN=LOC111463483 PE=3 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.1e-273
Identity = 474/475 (99.79%), Postives = 474/475 (99.79%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           VRRLKSFVTELTSITTSNHRNH NKICKNIKKNIFTKWVFRQSVQEANRKMQAER
Sbjct: 421 VRRLKSFVTELTSITTSNHRNHDNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 475

[Close]

BLAST of Carg18523-RA vs. ExPASy TrEMBL
Match: A0A6J1KRU5 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN=LOC111497071 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 9.7e-267
Identity = 461/475 (97.05%), Postives = 466/475 (98.11%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQITQ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKDAQ+RNLRVKGVLVTNPSNPLGTTM+RDELNLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVSAMEVLKERSS+DEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT +YI+ENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESD FESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           VRRLKSFVTEL S TTSNHRNH NKICKNIKKNIFTKWVFRQS QEANRKMQAER
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKKNIFTKWVFRQSAQEANRKMQAER 475

[Close]

BLAST of Carg18523-RA vs. ExPASy TrEMBL
Match: A0A6J1GGW6 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita moschata OX=3662 GN=LOC111453796 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 434/478 (90.79%), Postives = 455/478 (95.19%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYE NPF   SNP GIIQMGLAENQLSFDLLESWL+
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDG+SIFRELALFQDYHGLPAFKKALVEFMAEIRGNKV+FEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQ+T+ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTRPALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYK+A++RNLRVKGVLVTNPSNPLGTTMSR EL+LVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVSAMEVL+ERSS++EEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT NYITENQ+RLKQRQKMLVSGL+KAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHC+EPGWFR+CFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRLCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHR---NHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           +RRLKSFV EL S  + N +   N+ NK CKNIKKNIFTKWVFR SVQEANRK+Q ER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478

[Close]

BLAST of Carg18523-RA vs. ExPASy TrEMBL
Match: A0A6J1IUH1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita maxima OX=3661 GN=LOC111478535 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 3.7e-250
Identity = 434/478 (90.79%), Postives = 454/478 (94.98%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYE NPF   SNP GIIQMGLAENQLSFDLLESWL+
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDG+SIFRELALFQDYHGLPAFKKALVEFMAEIRGNKV+FEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQ+TQ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTQPALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYK+A++RNLRVKGVLVTNPSNPLGTTMSR EL+LVFDFITSKGIHLISDEIYSGTVFG 
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGF 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVSAMEVL+ERSSQ+EEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSQEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT NYI+ENQKRLKQRQKMLVSGL+KAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYISENQKRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHC++PGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSQPGWFRVCFANMSESTLKLA 420

Query: 421 VRRLKSFVTELTSITTSNHR---NHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           +RRLKSFV EL S  + N +   N+ NK CKN+KKNIFTKWVFR SVQEANRK+Q ER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNVKKNIFTKWVFRLSVQEANRKLQHER 478

[Close]

BLAST of Carg18523-RA vs. ExPASy TrEMBL
Match: I1VWI5 (1-aminocyclopropane-1-carboxylate synthase OS=Citrullus lanatus subsp. vulgaris OX=260674 GN=ACS1 PE=2 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.8e-249
Identity = 433/477 (90.78%), Postives = 452/477 (94.76%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MKMLSTKATCNSHGQDSSYFLGWEAYE NPF  T NP GIIQMGLAENQLSFDLLESWL+
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDETCNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKV+FEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQ+T+ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTKSALEQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYK+AQ+RNLRVKGVLVTNPSNPLGTTM+R EL+LVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAQTRNLRVKGVLVTNPSNPLGTTMTRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GFVSAMEVL+ERSS+DEEVWKRVHIVYSLSKDLGLPGFRVGAIYSND MVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDQMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT NYI+ENQKRLKQRQ+MLVSGL KAGI CL+SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYISENQKRLKQRQQMLVSGLGKAGIKCLESNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESEL+LWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMS+STLKLA
Sbjct: 361 WVDMRHLLESDTFESELKLWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSQSTLKLA 420

Query: 421 VRRLKSFVTELTSITTSN--HRNHHNKICKNIKKNIFTKWVFRQSVQEANRKMQAER 476
           +RRLKSFV EL S+ T N    N+ NK CKNIKKNIFTKWVFRQSVQEANRK+  ER
Sbjct: 421 IRRLKSFVRELRSLNTPNVVSTNYDNKFCKNIKKNIFTKWVFRQSVQEANRKLHHER 477

[Close]

BLAST of Carg18523-RA vs. TAIR 10
Match: AT5G65800.1 (ACC synthase 5 )

HSP 1 Score: 711.4 bits (1835), Expect = 4.7e-205
Identity = 346/463 (74.73%), Postives = 397/463 (85.75%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MK LSTK T N HGQDSSYFLGWE YE NP+    NPNG+IQMGLAENQL FDL+ESWL+
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAAS KR+G+SIFRELALFQDYHG+P FKKA+ EFM EIRGN+V+F+   IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQIT+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY+ AQ  +L+VKGVLVTNPSNPLGT ++R ELNL+ DFITSK IHLISDEIYSGT+FG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             F+S M+VLK++  +D EV KRVH+VYSLSKDLGLPGFRVGAIYSND+M+V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT+ Y+ ENQKRLK RQ+ LVSGL+ AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+EL+LWKKIVY V LNISPGSSCHCTEPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQS 464
           ++RLK+FV E T       R+ H ++ K+++K   + WVFR S
Sbjct: 421 LKRLKTFV-ESTDCGRMISRSSHERL-KSLRKKTVSNWVFRVS 461

[Close]

BLAST of Carg18523-RA vs. TAIR 10
Match: AT3G49700.1 (1-aminocyclopropane-1-carboxylate synthase 9 )

HSP 1 Score: 710.3 bits (1832), Expect = 1.0e-204
Identity = 349/463 (75.38%), Postives = 399/463 (86.18%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           MK LS K T N+HGQDSSYFLGWE YE NP+    NPNGIIQMGLAENQL FDL+E+WL+
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAA  K+DG+SIF+ELALFQDYHGLP FKKAL EFM EIRGN+V+F+ + IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQIT+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY+ AQ  +L+VKGVLVTNPSNPLGT ++R ELNL+ DFITSK IHLISDEIYSGTVFG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             FVS M+VLK+++ ++ EV KRVHIVYSLSKDLGLPGFRVGAIYSND+MVV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT+ Y+ ENQKRLK RQK LVSGL+ AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+ELELWKKIVY+V LNISPGSSCHCTEPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQS 464
           ++RLK +V E T       ++ H++I K+++K   + WVFR S
Sbjct: 421 MKRLKEYV-ESTDSRRVISKSSHDRI-KSLRKRTVSNWVFRVS 461

[Close]

BLAST of Carg18523-RA vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 688.7 bits (1776), Expect = 3.2e-198
Identity = 336/466 (72.10%), Postives = 392/466 (84.12%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           M +LS KA+CN+HGQDSSYF GWE YE NP+    NP+GIIQMGLAENQLSFDL+ESWL+
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           KNPDAA+F+R+G+SIFRELALFQDYHGLP+FK A+ +FM+E RGN+VSF  N +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           T ANETLMFCLA+ GDAFLLPTPYYPGFDRDLKWRTG EIVPI C S+N F+IT+ ALE+
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY+ AQ  NL+VKGVL+TNPSNPLGTT +R ELN + DFI+ K IHLISDEIYSGTVF +
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 SGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
            GF+S MEVLK+R  ++ +V+ RVHIVYSLSKDLGLPGFRVG IYSNDD VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360
           FGL+SSQTQYLLSA+LSDK FT NY+ ENQ RLK R K LVSGL+ AGI CL SNAGLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+S+TFE+E+ELWKKIVYEV LNISPGSSCHC EPGWFRVCFAN+SE TLK+A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 VRRLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQSVQE 467
           + RLK FV   +   T   ++ H ++ KN++K   + WVFR S  +
Sbjct: 421 LDRLKRFVDGPS--PTRRSQSEHQRL-KNLRKMKVSNWVFRLSFHD 463

[Close]

BLAST of Carg18523-RA vs. TAIR 10
Match: AT2G22810.1 (1-aminocyclopropane-1-carboxylate synthase 4 )

HSP 1 Score: 659.4 bits (1700), Expect = 2.1e-189
Identity = 328/469 (69.94%), Postives = 385/469 (82.09%), Query Frame = 0


Query: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60
           M  LS KATCNSHGQ SSYFLGWE YE NP+  T NP GIIQMGLAENQL FDLLESWL+
Sbjct: 1   MVQLSRKATCNSHGQVSSYFLGWEEYEKNPYDVTKNPQGIIQMGLAENQLCFDLLESWLA 60

Query: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120
           +N DAA FKRDG+S+FRELALFQDYHGL +FK A  +FM+E RGN+VSF++NN+VLTAGA
Sbjct: 61  QNTDAACFKRDGQSVFRELALFQDYHGLSSFKNAFADFMSENRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQ 180
           TSANETLMFCLA+ GDAFLLPTPYYPGFDRDLKWRTGVEIVPI  +S+N F+IT+ ALE+
Sbjct: 121 TSANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGVEIVPIQSSSTNGFRITKLALEE 180

Query: 181 AYKDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFIT-SKGIHLISDEIYSGTVFG 240
           AY+ A+  +L VKG+L+TNPSNPLGTT ++ ELN++FDFIT +K IHL+SDEIYSGTVF 
Sbjct: 181 AYEQAKKLDLNVKGILITNPSNPLGTTTTQTELNILFDFITKNKNIHLVSDEIYSGTVFN 240

Query: 241 SSGFVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300
           SS F+S ME+LK    ++ +V  RVHIV SLSKDLGLPGFRVGAIYSND  V++AATKMS
Sbjct: 241 SSEFISVMEILKNNQLENTDVLNRVHIVCSLSKDLGLPGFRVGAIYSNDKDVISAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLF 360
           SFGLVSSQTQYLLS++LSDKKFT NY+ ENQKRLK RQ+ LV GL+  GI CL SNAGLF
Sbjct: 301 SFGLVSSQTQYLLSSLLSDKKFTKNYLRENQKRLKNRQRKLVLGLEAIGIKCLKSNAGLF 360

Query: 361 CWVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKL 420
           CWVDMR LL S TFE+E++LWKKIVYEV LNISPGSSCHC EPGWFRVCFANM + TLKL
Sbjct: 361 CWVDMRPLLRSKTFEAEMDLWKKIVYEVKLNISPGSSCHCEEPGWFRVCFANMIDETLKL 420

Query: 421 AVRRLKSFVTELTSITTSNHRNHHNKICKNIKKNI--FTKWVFRQSVQE 467
           A++RLK  V +  S +    ++   ++  + KK +   + WVFR S  +
Sbjct: 421 ALKRLKMLVDDENS-SRRCQKSKSERLNGSRKKTMSNVSNWVFRLSFHD 468

[Close]

BLAST of Carg18523-RA vs. TAIR 10
Match: AT4G08040.1 (1-aminocyclopropane-1-carboxylate synthase 11 )

HSP 1 Score: 613.6 bits (1581), Expect = 1.3e-175
Identity = 293/461 (63.56%), Postives = 371/461 (80.48%), Query Frame = 0


Query: 3   MLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLSKN 62
           MLS+K   +SHGQDSSYFLGW+ YE NPFH + N +GI+QMGLAENQLSFDL+E WL ++
Sbjct: 1   MLSSKVVGDSHGQDSSYFLGWQEYEKNPFHESFNTSGIVQMGLAENQLSFDLIEKWLEEH 60

Query: 63  PDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGATS 122
           P+    K++ +S+FR+LALFQDYHGLPAFK A+ +FM +IR NKV F+ N +VLTAG+TS
Sbjct: 61  PEVLGLKKNDESVFRQLALFQDYHGLPAFKDAMAKFMGKIRENKVKFDTNKMVLTAGSTS 120

Query: 123 ANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQITQRALEQAY 182
           ANETLMFCLA  GDAFL+P PYYPGFDRDLKWRTGVEIVPIHC SSN ++IT+ ALE AY
Sbjct: 121 ANETLMFCLANPGDAFLIPAPYYPGFDRDLKWRTGVEIVPIHCVSSNGYKITEDALEDAY 180

Query: 183 KDAQSRNLRVKGVLVTNPSNPLGTTMSRDELNLVFDFITSKGIHLISDEIYSGTVFGSSG 242
           + A   NL VKGVL+TNPSNPLGT+ +R+EL+L+  F ++K IH++SDEIYSGTVF S  
Sbjct: 181 ERALKHNLNVKGVLITNPSNPLGTSTTREELDLLLTFTSTKKIHMVSDEIYSGTVFDSPE 240

Query: 243 FVSAMEVLKERSSQDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSSFG 302
           F S +EV K+++   +    ++H+VYSLSKDLGLPGFRVG IYSN++ VV+AATKMSSFG
Sbjct: 241 FTSVLEVAKDKNMGLD---GKIHVVYSLSKDLGLPGFRVGLIYSNNEKVVSAATKMSSFG 300

Query: 303 LVSSQTQYLLSAMLSDKKFTTNYITENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFCWV 362
           L+SSQTQ+LL+ +LSD++FTTNY+ EN+KRL++R+  LVSGL++AGI+CL SNAGLFCWV
Sbjct: 301 LISSQTQHLLANLLSDERFTTNYLEENKKRLRERKDRLVSGLKEAGISCLKSNAGLFCWV 360

Query: 363 DMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLAVR 422
           D+RHLL+S+TFE+E  LW KIV EVGLNISPGSSCHC EPGWFRVCFANMS+ T+++A+ 
Sbjct: 361 DLRHLLKSNTFEAEHSLWTKIVCEVGLNISPGSSCHCDEPGWFRVCFANMSDQTMEVAMD 420

Query: 423 RLKSFVTELTSITTSNHRNHHNKICKNIKKNIFTKWVFRQS 464
           R+K FV        +N       +    ++++  KWV + S
Sbjct: 421 RVKGFV------DNNNGGKQKRTMWDTRRRSLINKWVSKLS 452

The following BLAST results are available for this feature:

BLAST of Carg18523-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6594949.1	2.0e-274	100.00	1-aminocyclopropane-1-carboxylate synthase 101, partial [Cucurbita argyrosperma ...	[more]
XP_022963198.1	2.2e-273	99.79	1-aminocyclopropane-1-carboxylate synthase CMA101 [Cucurbita moschata]	[more]
XP_023518664.1	1.4e-272	99.37	1-aminocyclopropane-1-carboxylate synthase CMA101 [Cucurbita pepo subsp. pepo]	[more]
XP_023003465.1	2.0e-266	97.05	1-aminocyclopropane-1-carboxylate synthase CMA101 [Cucurbita maxima] >Q00257.1 R...	[more]
XP_023544912.1	6.9e-251	91.21	1-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita pepo subsp. pe...	[more]

BLAST of Carg18523-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00257	2.6e-269	97.05	1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN...	[more]
Q42881	1.1e-211	76.17	1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=...	[more]
P37821	9.2e-206	74.73	1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 P...	[more]
Q37001	6.6e-204	74.73	1-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9M2Y8	1.5e-203	75.38	1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Carg18523-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HH23	1.1e-273	99.79	1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1KRU5	9.7e-267	97.05	1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1GGW6	2.8e-250	90.79	1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita moschata OX=...	[more]
A0A6J1IUH1	3.7e-250	90.79	1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita maxima OX=36...	[more]
I1VWI5	1.8e-249	90.78	1-aminocyclopropane-1-carboxylate synthase OS=Citrullus lanatus subsp. vulgaris ...	[more]

BLAST of Carg18523-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65800.1	4.7e-205	74.73	ACC synthase 5	[more]
AT3G49700.1	1.0e-204	75.38	1-aminocyclopropane-1-carboxylate synthase 9	[more]
AT4G37770.1	3.2e-198	72.10	1-amino-cyclopropane-1-carboxylate synthase 8	[more]
AT2G22810.1	2.1e-189	69.94	1-aminocyclopropane-1-carboxylate synthase 4	[more]
AT4G08040.1	1.3e-175	63.56	1-aminocyclopropane-1-carboxylate synthase 11	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00753	ACCSYNTHASE	coord: 43..69 score: 66.12 coord: 263..287 score: 83.28 coord: 135..156 score: 86.36 coord: 76..92 score: 74.54 coord: 295..318 score: 81.76 coord: 225..248 score: 64.55 coord: 113..133 score: 66.9 coord: 189..213 score: 70.16
None	No IPR available	PANTHER	PTHR43795	BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED	coord: 1..442
None	No IPR available	PANTHER	PTHR43795:SF41	1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE	coord: 1..442
None	No IPR available	CDD	cd00609	AAT_like	coord: 45..425 e-value: 2.26566E-65 score: 212.587
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 63..318 e-value: 1.3E-169 score: 566.1
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 29..423 e-value: 1.3E-169 score: 566.1
IPR004839	Aminotransferase, class I/classII	PFAM	PF00155	Aminotran_1_2	coord: 41..424 e-value: 4.4E-106 score: 355.1
IPR004838	Aminotransferases, class-I, pyridoxal-phosphate-binding site	PROSITE	PS00105	AA_TRANSFER_CLASS_1	coord: 269..282
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 3..428

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg18523	Carg18523	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg18523-RA:4	Carg18523-RA:4	exon
Carg18523-RA:3	Carg18523-RA:3	exon
Carg18523-RA:2	Carg18523-RA:2	exon
Carg18523-RA:1	Carg18523-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg18523-RA:cds	Carg18523-RA:cds_4	CDS
Carg18523-RA:cds	Carg18523-RA:cds_3	CDS
Carg18523-RA:cds	Carg18523-RA:cds_2	CDS
Carg18523-RA:cds	Carg18523-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg18523-RA	Carg18523-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0042218	1-aminocyclopropane-1-carboxylate biosynthetic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0016847	1-aminocyclopropane-1-carboxylate synthase activity
molecular_function	GO:0008483	transaminase activity