Carg16003-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16003-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ethylene-responsive transcription factor ERF060-like
Location	Carg_Chr07: 2377497 .. 2378513 (+)
Sequence length	1017
RNA-Seq Expression	Carg16003-RA
Synteny	Carg16003-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCGGCTTTAGATTTTTGCACTGGAATTTCTGCGCCGGTTTACTCTTCGGATCCTTTTTGCGAAGAGTTAATGAAAGCACTTCAACCTTTTATGAAGAGTGCTGTTTCAACCTCTTCTTCTTCTTCTGTTTCTTATCAGCCTAATGTGAACCCTGATTTTTGCTCCCCGTCGAGCATCCGCTTGTTTCCTCAAGCGTGTTCGGGAATTGAACAAATGGGTCTTGAGCAATCGGGTCCAATCGGGCTAAACAATCCCACCCCTTCACAAGTTCTACAAATTCAAGCTCAAATCCAACTACCCTCACAAACTATGTCTTCCTTCCCTTCTTCTTTTTCATTCCGGTCTCGAGTTTCGTCCCAATACCATAGCGTCCTTGCTCCCAAATCCATTTCGATGAAGCACACCGGCTCTCCGACTAAGCCCACTAGAGTTTACAGGGGAATCCGGCAGCGCCACTGGGGAAAATGGGTCGCCGAAATTAGACTCCCCAAGAACCGAACTAGACTCTGGCTTGGCACTTTCGACACGGCCGAGGAGGCGGCTTTAGCTTATGACAAAGCTGCTTACAAGCTCCGTGGCGACTTGGCTCGCCTCAATTTTCCACATCTCAAACATCAATTCGGGGACTTCGAGCCTCTTCACTCTTCTGTGGACGCGAAACTTCAAGCAATTTGTCAAAGCTTGAAACAGGGGGAAACAGAGAAGCCACCGAGCGCTCCACCTCCATCTGAATCGAACTCAGTGGTTAACAATTTTAAGGAAGCGGAATTGAAAATCGAAGCGGAGACTTCATCTTCATCTTCATCTTCATGGTCATCTCCTTCGCTGTGTGAAGATTTCTCCACCGGGTCCTCCCCTGAGTCGAGTATTAGCTTCTTGGATTTCTCAGATTCGCAATGGGGTGAAGGAGAAGCATTCTGTTTGGAAAAGTATCCTTCCGTTGAAATTGATTGGGCAGCTCTGAACCAGCTGGGCGAATCAGAGTGTTTAATTAGTAATATTTCAATGTAA

mRNA sequence

ATGGCAGCGGCTTTAGATTTTTGCACTGGAATTTCTGCGCCGGTTTACTCTTCGGATCCTTTTTGCGAAGAGTTAATGAAAGCACTTCAACCTTTTATGAAGAGTGCTGTTTCAACCTCTTCTTCTTCTTCTGTTTCTTATCAGCCTAATGTGAACCCTGATTTTTGCTCCCCGTCGAGCATCCGCTTGTTTCCTCAAGCGTGTTCGGGAATTGAACAAATGGGTCTTGAGCAATCGGGTCCAATCGGGCTAAACAATCCCACCCCTTCACAAGTTCTACAAATTCAAGCTCAAATCCAACTACCCTCACAAACTATGTCTTCCTTCCCTTCTTCTTTTTCATTCCGGTCTCGAGTTTCGTCCCAATACCATAGCGTCCTTGCTCCCAAATCCATTTCGATGAAGCACACCGGCTCTCCGACTAAGCCCACTAGAGTTTACAGGGGAATCCGGCAGCGCCACTGGGGAAAATGGGTCGCCGAAATTAGACTCCCCAAGAACCGAACTAGACTCTGGCTTGGCACTTTCGACACGGCCGAGGAGGCGGCTTTAGCTTATGACAAAGCTGCTTACAAGCTCCGTGGCGACTTGGCTCGCCTCAATTTTCCACATCTCAAACATCAATTCGGGGACTTCGAGCCTCTTCACTCTTCTGTGGACGCGAAACTTCAAGCAATTTGTCAAAGCTTGAAACAGGGGGAAACAGAGAAGCCACCGAGCGCTCCACCTCCATCTGAATCGAACTCAGTGGTTAACAATTTTAAGGAAGCGGAATTGAAAATCGAAGCGGAGACTTCATCTTCATCTTCATCTTCATGGTCATCTCCTTCGCTGTGTGAAGATTTCTCCACCGGGTCCTCCCCTGAGTCGAGTATTAGCTTCTTGGATTTCTCAGATTCGCAATGGGGTGAAGGAGAAGCATTCTGTTTGGAAAAGTATCCTTCCGTTGAAATTGATTGGGCAGCTCTGAACCAGCTGGGCGAATCAGAGTGTTTAATTAGTAATATTTCAATGTAA

Coding sequence (CDS)

ATGGCAGCGGCTTTAGATTTTTGCACTGGAATTTCTGCGCCGGTTTACTCTTCGGATCCTTTTTGCGAAGAGTTAATGAAAGCACTTCAACCTTTTATGAAGAGTGCTGTTTCAACCTCTTCTTCTTCTTCTGTTTCTTATCAGCCTAATGTGAACCCTGATTTTTGCTCCCCGTCGAGCATCCGCTTGTTTCCTCAAGCGTGTTCGGGAATTGAACAAATGGGTCTTGAGCAATCGGGTCCAATCGGGCTAAACAATCCCACCCCTTCACAAGTTCTACAAATTCAAGCTCAAATCCAACTACCCTCACAAACTATGTCTTCCTTCCCTTCTTCTTTTTCATTCCGGTCTCGAGTTTCGTCCCAATACCATAGCGTCCTTGCTCCCAAATCCATTTCGATGAAGCACACCGGCTCTCCGACTAAGCCCACTAGAGTTTACAGGGGAATCCGGCAGCGCCACTGGGGAAAATGGGTCGCCGAAATTAGACTCCCCAAGAACCGAACTAGACTCTGGCTTGGCACTTTCGACACGGCCGAGGAGGCGGCTTTAGCTTATGACAAAGCTGCTTACAAGCTCCGTGGCGACTTGGCTCGCCTCAATTTTCCACATCTCAAACATCAATTCGGGGACTTCGAGCCTCTTCACTCTTCTGTGGACGCGAAACTTCAAGCAATTTGTCAAAGCTTGAAACAGGGGGAAACAGAGAAGCCACCGAGCGCTCCACCTCCATCTGAATCGAACTCAGTGGTTAACAATTTTAAGGAAGCGGAATTGAAAATCGAAGCGGAGACTTCATCTTCATCTTCATCTTCATGGTCATCTCCTTCGCTGTGTGAAGATTTCTCCACCGGGTCCTCCCCTGAGTCGAGTATTAGCTTCTTGGATTTCTCAGATTCGCAATGGGGTGAAGGAGAAGCATTCTGTTTGGAAAAGTATCCTTCCGTTGAAATTGATTGGGCAGCTCTGAACCAGCTGGGCGAATCAGAGTGTTTAATTAGTAATATTTCAATGTAA

Protein sequence

MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVSSQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM

Homology

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BLAST of Carg16003-RA vs. NCBI nr
Match: KAG7026835.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 666.4 bits (1718), Expect = 1.3e-187
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60
           MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS
Sbjct: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60

Query: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVS 120
           IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVS
Sbjct: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVS 120

Query: 121 SQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180
           SQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE
Sbjct: 121 SQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180

Query: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240
           EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS
Sbjct: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240

Query: 241 APPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFSDS 300
           APPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFSDS
Sbjct: 241 APPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFSDS 300

Query: 301 QWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 339
           QWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM
Sbjct: 301 QWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 338

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BLAST of Carg16003-RA vs. NCBI nr
Match: KAG6594870.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 333/341 (97.65%), Postives = 335/341 (98.24%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAV---STSSSSSVSYQPNVNPDFCS 60
           MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAV   S+SSSSSVSYQPN+NPDFCS
Sbjct: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSSSSSSSSSSVSYQPNLNPDFCS 60

Query: 61  PSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRS 120
           PSSIRLFPQACSGIEQ GLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRS
Sbjct: 61  PSSIRLFPQACSGIEQTGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRS 120

Query: 121 RVSSQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFD 180
           RVSSQYHSVLAPKSI MKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFD
Sbjct: 121 RVSSQYHSVLAPKSIPMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFD 180

Query: 181 TAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEK 240
           TAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEK
Sbjct: 181 TAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEK 240

Query: 241 PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDF 300
           PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSL EDFSTGSSPESSISFLDF
Sbjct: 241 PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLWEDFSTGSSPESSISFLDF 300

Query: 301 SDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 339
           SDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM
Sbjct: 301 SDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 341

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BLAST of Carg16003-RA vs. NCBI nr
Match: XP_023517236.1 (ethylene-responsive transcription factor RAP2-13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.7 bits (1654), Expect = 3.5e-180
Identity = 326/336 (97.02%), Postives = 332/336 (98.81%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAV---STSSSSSVSYQPNVNPDFCS 60
           MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAV   S+SSSSSVSYQPNVNPDFCS
Sbjct: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSSSSSSSSSSVSYQPNVNPDFCS 60

Query: 61  PSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRS 120
           PSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQT+SSFPSSFSF+S
Sbjct: 61  PSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTLSSFPSSFSFQS 120

Query: 121 RVSSQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFD 180
           RVSSQYHSVLAPKSI MK+TGSPTKPTR+YRGIRQRHWGKWVAEIRLPKNRTRLWLGTFD
Sbjct: 121 RVSSQYHSVLAPKSIPMKYTGSPTKPTRIYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFD 180

Query: 181 TAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEK 240
           TAEEAALAYDKAAYKLRGDLARLNFPHLKH+FGDFEPLHSSVDAKLQAICQSLKQGETEK
Sbjct: 181 TAEEAALAYDKAAYKLRGDLARLNFPHLKHKFGDFEPLHSSVDAKLQAICQSLKQGETEK 240

Query: 241 PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDF 300
           PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDF
Sbjct: 241 PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDF 300

Query: 301 SDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLI 334
           SDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLI
Sbjct: 301 SDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLI 336

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BLAST of Carg16003-RA vs. NCBI nr
Match: XP_022963399.1 (ethylene-responsive transcription factor ERF060-like [Cucurbita moschata])

HSP 1 Score: 641.3 bits (1653), Expect = 4.5e-180
Identity = 327/332 (98.49%), Postives = 329/332 (99.10%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60
           MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS
Sbjct: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60

Query: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVS 120
           IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSF+SRVS
Sbjct: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFQSRVS 120

Query: 121 SQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180
           SQYHSVLAPKSI MKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE
Sbjct: 121 SQYHSVLAPKSIPMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180

Query: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240
           EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS
Sbjct: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240

Query: 241 APPPSESNSVVNNFKEAELKIEAET--SSSSSSSWSSPSLCEDFSTGSSPESSISFLDFS 300
           +PPPSESNSVVNNFKEAELKIEAET  SSSSSSSWSSPSLCEDFSTGSSPESSISFLDFS
Sbjct: 241 SPPPSESNSVVNNFKEAELKIEAETSSSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFS 300

Query: 301 DSQWGEGEAFCLEKYPSVEIDWAALNQLGESE 331
           DSQWGEGEAFCLEKYPSVEIDWAALNQLGESE
Sbjct: 301 DSQWGEGEAFCLEKYPSVEIDWAALNQLGESE 332

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BLAST of Carg16003-RA vs. NCBI nr
Match: XP_023004027.1 (ethylene-responsive transcription factor ERF060-like [Cucurbita maxima])

HSP 1 Score: 626.7 bits (1615), Expect = 1.2e-175
Identity = 324/344 (94.19%), Postives = 331/344 (96.22%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60
           MAAALDF TGISAPVYSSDPF EELMKAL+PFMKSAVS+SSSSSVSYQPNVNPDF SPS+
Sbjct: 1   MAAALDFYTGISAPVYSSDPFFEELMKALKPFMKSAVSSSSSSSVSYQPNVNPDFRSPST 60

Query: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVS 120
           IRLFPQACSGIEQ+GLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSF+SRVS
Sbjct: 61  IRLFPQACSGIEQVGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFQSRVS 120

Query: 121 SQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180
           SQYHS+LAPKSI MKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE
Sbjct: 121 SQYHSILAPKSIPMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180

Query: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240
           EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEK PS
Sbjct: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKSPS 240

Query: 241 APPPSESNSVVNNFKEAELKIEAET------SSSSSSSWSSPSLCEDFSTGSSPESSISF 300
           APPPSESNSVV+ FKEAELKIEAET      SSSSSSSWSSPSLCEDFST SSPESSISF
Sbjct: 241 APPPSESNSVVDKFKEAELKIEAETSSSSSSSSSSSSSWSSPSLCEDFSTESSPESSISF 300

Query: 301 LDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 339
           LDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM
Sbjct: 301 LDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 344

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BLAST of Carg16003-RA vs. ExPASy Swiss-Prot
Match: Q8H1E4 (Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana OX=3702 GN=RAP2-4 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-57
Identity = 152/327 (46.48%), Postives = 201/327 (61.47%), Query Frame = 0


Query: 24  ELMKALQPFMKS-AVSTSSSSSVSYQPNVNPD-FCSPSSIRLFPQACSGIEQMG----LE 83
           ELM AL PF+KS + S SSSS+ S    ++P  F  P     +P +    +       L+
Sbjct: 20  ELMDALVPFIKSVSDSPSSSSAASASAFLHPSAFSLPPLPGYYPDSTFLTQPFSYGSDLQ 79

Query: 84  QSGP-IGLNNPTPSQVLQIQAQIQLP-SQTMSSFPSSFSFRSRVSSQYHSVLAPKSISMK 143
           Q+G  IGLNN + SQ+ QIQ+QI  P   T  +  +SFS          ++L+PK + MK
Sbjct: 80  QTGSLIGLNNLSSSQIHQIQSQIHHPLPPTHHNNNNSFS----------NLLSPKPLLMK 139

Query: 144 H-----------TGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAL 203
                       +G P+KPT++YRG+RQRHWGKWVAEIRLP+NRTRLWLGTFDTAEEAAL
Sbjct: 140 QSGVAGSCFAYGSGVPSKPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAAL 199

Query: 204 AYDKAAYKLRGDLARLNFPHLKH-------QFGDFEPLHSSVDAKLQAICQSLKQGETEK 263
           AYDKAAYKLRGD ARLNFP+L+H        FG+++PLHSSVDAKL+AIC+S+   ET+K
Sbjct: 200 AYDKAAYKLRGDFARLNFPNLRHNGSHIGGDFGEYKPLHSSVDAKLEAICKSM--AETQK 259

Query: 264 PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDF 323
              +   S+      +  +   K++AE +S S       +  E+ + GSSP S ++F D 
Sbjct: 260 QDKSTKSSKKREKKVSSPDLSEKVKAEENSVSIGGSPPVTEFEESTAGSSPLSDLTFADP 319

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BLAST of Carg16003-RA vs. ExPASy Swiss-Prot
Match: O65665 (Ethylene-responsive transcription factor ERF060 OS=Arabidopsis thaliana OX=3702 GN=ERF060 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 149/325 (45.85%), Postives = 187/325 (57.54%), Query Frame = 0


Query: 17  SSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSSIRLFPQACSGIEQMGL 76
           ++DPF EELMKALQP+     + + SSS +Y   V                       G 
Sbjct: 10  NTDPFQEELMKALQPY----TTNTDSSSPTYSNTV----------------------FGF 69

Query: 77  EQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVSSQYHSVLAPKSISMKH 136
            Q+  +GLN  TP Q+ QIQ Q+      +S  P+               LAPK + MK+
Sbjct: 70  NQTTSLGLNQLTPYQIHQIQNQLNQRRNIIS--PN---------------LAPKPVPMKN 129

Query: 137 TGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKLRGD 196
             +     ++YRG+RQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAA+AYD AAYKLRG+
Sbjct: 130 MTA----QKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAMAYDLAAYKLRGE 189

Query: 197 LARLNFPHLKHQFG------DFEPLHSSVDAKLQAICQSLKQGETEKPPSA-----PPPS 256
            ARLNFP  +H+ G       F PLHSSVDAKLQ ICQSL++ E    P +     PP +
Sbjct: 190 FARLNFPQFRHEDGYYGGGSCFNPLHSSVDAKLQEICQSLRKTEDIDLPCSETELFPPKT 249

Query: 257 ESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSI-SFLDFSDSQWGE 316
           E       ++E+E         S  +S+S  S  E     SSPES I +FLDFSDS + E
Sbjct: 250 E-------YQESEYGF----LRSDENSFSDESHVE----SSSPESGITTFLDFSDSGFDE 272

Query: 317 GEAFCLEKYPSVEIDWAALNQLGES 330
             +F LEK+PSVEIDW A+++L ES
Sbjct: 310 IGSFGLEKFPSVEIDWDAISKLSES 272

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BLAST of Carg16003-RA vs. ExPASy Swiss-Prot
Match: Q9FJQ2 (Ethylene-responsive transcription factor ERF057 OS=Arabidopsis thaliana OX=3702 GN=ERF057 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.5e-50
Identity = 135/309 (43.69%), Postives = 186/309 (60.19%), Query Frame = 0


Query: 21  FCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSSIRLFPQACSGIEQMGLEQSG 80
           + +E M+AL+PFMK   S+S+S+S + +P + P+F  P++ ++ P +          Q+G
Sbjct: 8   YVDEFMEALEPFMKVTSSSSTSNSSNPKP-LTPNFI-PNNDQVLPVS---------NQTG 67

Query: 81  PIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVSSQYHSVLAPKSISMKHTGSP 140
           PIGLN  TP+Q+LQIQ ++ L  Q  S        R R  S    +L  K  SMK     
Sbjct: 68  PIGLNQLTPTQILQIQTELHL-RQNQS--------RRRAGSH---LLTAKPTSMKKIDVA 127

Query: 141 TKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKLRGDLARL 200
           TKP ++YRG+RQR WGKWVAEIRLPKNRTRLWLGTF+TA+EAALAYD+AA+K+RGD ARL
Sbjct: 128 TKPVKLYRGVRQRQWGKWVAEIRLPKNRTRLWLGTFETAQEAALAYDQAAHKIRGDNARL 187

Query: 201 NFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKP-PSAPPPSESNSVVNNFKEAEL 260
           NFP +  Q    + L  S++AK+++IC S     ++ P P     +++  V++ F + E 
Sbjct: 188 NFPDIVRQGHYKQILSPSINAKIESICNS-----SDLPLPQIEKQNKTEEVLSGFSKPEK 247

Query: 261 KIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFSDSQWGEGEAFC--LEKYPSVE 320
             E E        +S          GSSPES I+ LDFS     E E+F   L KYPS+E
Sbjct: 248 --EPEFGEIYGCGYS----------GSSPESDITLLDFSSDCVKEDESFLMGLHKYPSLE 276

Query: 321 IDWAALNQL 327
           IDW A+ +L
Sbjct: 308 IDWDAIEKL 276

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BLAST of Carg16003-RA vs. ExPASy Swiss-Prot
Match: Q9LM15 (Ethylene-responsive transcription factor RAP2-13 OS=Arabidopsis thaliana OX=3702 GN=RAP2-13 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.6e-49
Identity = 125/237 (52.74%), Postives = 155/237 (65.40%), Query Frame = 0


Query: 99  IQLPSQTMSSF----PSSFSFRSRVSSQYHSVLAPKSISMKHTG-SPTKPTRVYRGIRQR 158
           I+ PS   S+F    P+  S+ S + S  H  L+PK +SMK TG S  KPT++YRG+RQR
Sbjct: 31  IKSPSNDSSAFAFSLPAPISYGSDLHSFSHH-LSPKPVSMKQTGTSAAKPTKLYRGVRQR 90

Query: 159 HWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFE 218
           HWGKWVAEIRLP+NRTRLWLGTFDTAEEAALAYDKAAYKLRGD ARLNFP L+H   +++
Sbjct: 91  HWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPDLRHN-DEYQ 150

Query: 219 PLHSSVDAKLQAICQSLKQGETEKPPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSW 278
           PL SSVDAKL+AICQ+L +  T+K   +   S S           +K+  E  SS+    
Sbjct: 151 PLQSSVDAKLEAICQNLAE-TTQKQVRSTKKSSSR---KRSSTVAVKLPEEDYSSAG--- 210

Query: 279 SSPSLCEDFSTG--SSPESSISFLDFSDS---QWGEGEAFCLEKYPSVEIDWAALNQ 326
           SSP L E + +G  SSP S ++F D  +     W E     LEKYPS EIDW ++ Q
Sbjct: 211 SSPLLTESYGSGGSSSPLSELTFGDTEEEIQPPWNEN---ALEKYPSYEIDWDSILQ 255

[Close]

BLAST of Carg16003-RA vs. ExPASy Swiss-Prot
Match: Q9SIE4 (Ethylene-responsive transcription factor ERF056 OS=Arabidopsis thaliana OX=3702 GN=ERF056 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-47
Identity = 124/268 (46.27%), Postives = 166/268 (61.94%), Query Frame = 0


Query: 75  GLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVSSQYHSVLAPKSISM 134
           G+ +S P+GLN  TP Q+ QIQ Q+     T+S+                  L+P  I M
Sbjct: 16  GVNKSMPLGLNQLTPYQIHQIQNQLNHRRSTISN------------------LSPNRIRM 75

Query: 135 KH-TGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKL 194
           K+ T S +K   +YRG+RQRHWGKWVAEIRLPKNRTRLWLGTF+TAE+AALAYD+AA++L
Sbjct: 76  KNLTPSTSKTKNLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFETAEKAALAYDQAAFQL 135

Query: 195 RGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPSAPPPSESNSVVNN 254
           RGD+A+LNFP+L H+  D  PL SSVD KLQAIC+SL+  +TE+  S    ++  SV + 
Sbjct: 136 RGDIAKLNFPNLIHE--DMNPLPSSVDTKLQAICKSLR--KTEEICSVSDQTKEYSVYSV 195

Query: 255 FKEAEL-----------KIEAETSS-SSSSSWSSPSLCEDFSTGSSPESSISFLDFSDSQ 314
             + EL           +   ET+  S+ S  S  +   D S      S  +FLDFSD++
Sbjct: 196 SDKTELFLPKAELFLPKREHLETNELSNESPRSDETSLLDESQAEYSSSDKTFLDFSDTE 255

Query: 315 WGEGEAFCLEKYPSVEIDWAALNQLGES 330
           + E  +F L K+PSVEIDW A+++L  S
Sbjct: 256 FEEIGSFGLRKFPSVEIDWDAISKLANS 261

[Close]

BLAST of Carg16003-RA vs. ExPASy TrEMBL
Match: A0A6J1HK00 (ethylene-responsive transcription factor ERF060-like OS=Cucurbita moschata OX=3662 GN=LOC111463614 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.2e-180
Identity = 327/332 (98.49%), Postives = 329/332 (99.10%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60
           MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS
Sbjct: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60

Query: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVS 120
           IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSF+SRVS
Sbjct: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFQSRVS 120

Query: 121 SQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180
           SQYHSVLAPKSI MKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE
Sbjct: 121 SQYHSVLAPKSIPMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180

Query: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240
           EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS
Sbjct: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240

Query: 241 APPPSESNSVVNNFKEAELKIEAET--SSSSSSSWSSPSLCEDFSTGSSPESSISFLDFS 300
           +PPPSESNSVVNNFKEAELKIEAET  SSSSSSSWSSPSLCEDFSTGSSPESSISFLDFS
Sbjct: 241 SPPPSESNSVVNNFKEAELKIEAETSSSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFS 300

Query: 301 DSQWGEGEAFCLEKYPSVEIDWAALNQLGESE 331
           DSQWGEGEAFCLEKYPSVEIDWAALNQLGESE
Sbjct: 301 DSQWGEGEAFCLEKYPSVEIDWAALNQLGESE 332

[Close]

BLAST of Carg16003-RA vs. ExPASy TrEMBL
Match: A0A6J1KP93 (ethylene-responsive transcription factor ERF060-like OS=Cucurbita maxima OX=3661 GN=LOC111497459 PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 5.6e-176
Identity = 324/344 (94.19%), Postives = 331/344 (96.22%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSS 60
           MAAALDF TGISAPVYSSDPF EELMKAL+PFMKSAVS+SSSSSVSYQPNVNPDF SPS+
Sbjct: 1   MAAALDFYTGISAPVYSSDPFFEELMKALKPFMKSAVSSSSSSSVSYQPNVNPDFRSPST 60

Query: 61  IRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVS 120
           IRLFPQACSGIEQ+GLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSF+SRVS
Sbjct: 61  IRLFPQACSGIEQVGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFQSRVS 120

Query: 121 SQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180
           SQYHS+LAPKSI MKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE
Sbjct: 121 SQYHSILAPKSIPMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAE 180

Query: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPS 240
           EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEK PS
Sbjct: 181 EAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKSPS 240

Query: 241 APPPSESNSVVNNFKEAELKIEAET------SSSSSSSWSSPSLCEDFSTGSSPESSISF 300
           APPPSESNSVV+ FKEAELKIEAET      SSSSSSSWSSPSLCEDFST SSPESSISF
Sbjct: 241 APPPSESNSVVDKFKEAELKIEAETSSSSSSSSSSSSSWSSPSLCEDFSTESSPESSISF 300

Query: 301 LDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 339
           LDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM
Sbjct: 301 LDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESECLISNISM 344

[Close]

BLAST of Carg16003-RA vs. ExPASy TrEMBL
Match: A0A5A7T4I4 (Ethylene-responsive transcription factor ERF060 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002710 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.4e-134
Identity = 267/354 (75.42%), Postives = 286/354 (80.79%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSS-------------SVSY 60
           MAAA+D  +G S PVY SDPF EELMKALQPFMKSA+STSSSS             SVS 
Sbjct: 1   MAAAIDVYSGTSTPVYYSDPFSEELMKALQPFMKSAISTSSSSSSSSYSPSPPPHPSVSS 60

Query: 61  QPNVNPDFCSPSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMS 120
           QP + PDFCSPSS RLF Q  SGIEQMG EQSGPIGLNNPTPSQ+LQIQAQIQLPS TMS
Sbjct: 61  QPRLIPDFCSPSSTRLFSQGFSGIEQMGFEQSGPIGLNNPTPSQILQIQAQIQLPSPTMS 120

Query: 121 SFPSSFSFRSRVSSQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKN 180
           SF SS    S   SQYH+ L PKS  MKH GSP KPT++YRG+RQRHWGKWVAEIRLPKN
Sbjct: 121 SFSSS---SSSFQSQYHNFLTPKSFPMKHIGSPPKPTKLYRGVRQRHWGKWVAEIRLPKN 180

Query: 181 RTRLWLGTFDTAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAIC 240
           RTRLWLGTFDTAEEAALAYD+AAYKLRGD ARLNFPHLKHQFGDF+PLH SVDAKLQ+IC
Sbjct: 181 RTRLWLGTFDTAEEAALAYDQAAYKLRGDFARLNFPHLKHQFGDFKPLHPSVDAKLQSIC 240

Query: 241 QSLKQGET-------EKPPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCE 300
           QSLKQG+T       EKP + P PSESNS VNNF E ELK E ETS SSSS  SSPS  E
Sbjct: 241 QSLKQGKTEVCSVEDEKPTTIPLPSESNSAVNNFWEPELKSEVETSFSSSS--SSPSRSE 300

Query: 301 DF--STGSSPESSISFLDFSDSQWGEG-EAFCLEKYPSVEIDWAALNQLGESEC 332
           D+  STGSSP+SSISFLDFSDSQWGEG EAFCLEK+PS+EIDWAAL+QL ESEC
Sbjct: 301 DYSTSTGSSPDSSISFLDFSDSQWGEGVEAFCLEKFPSIEIDWAALSQLAESEC 349

[Close]

BLAST of Carg16003-RA vs. ExPASy TrEMBL
Match: A0A1S3B297 (ethylene-responsive transcription factor ERF060 OS=Cucumis melo OX=3656 GN=LOC103484983 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.4e-134
Identity = 267/354 (75.42%), Postives = 286/354 (80.79%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVSTSSSS-------------SVSY 60
           MAAA+D  +G S PVY SDPF EELMKALQPFMKSA+STSSSS             SVS 
Sbjct: 1   MAAAIDVYSGTSTPVYYSDPFSEELMKALQPFMKSAISTSSSSSSSSYSPSPPPHPSVSS 60

Query: 61  QPNVNPDFCSPSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMS 120
           QP + PDFCSPSS RLF Q  SGIEQMG EQSGPIGLNNPTPSQ+LQIQAQIQLPS TMS
Sbjct: 61  QPRLIPDFCSPSSTRLFSQGFSGIEQMGFEQSGPIGLNNPTPSQILQIQAQIQLPSPTMS 120

Query: 121 SFPSSFSFRSRVSSQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKN 180
           SF SS    S   SQYH+ L PKS  MKH GSP KPT++YRG+RQRHWGKWVAEIRLPKN
Sbjct: 121 SFSSS---SSSFQSQYHNFLTPKSFPMKHIGSPPKPTKLYRGVRQRHWGKWVAEIRLPKN 180

Query: 181 RTRLWLGTFDTAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAIC 240
           RTRLWLGTFDTAEEAALAYD+AAYKLRGD ARLNFPHLKHQFGDF+PLH SVDAKLQ+IC
Sbjct: 181 RTRLWLGTFDTAEEAALAYDQAAYKLRGDFARLNFPHLKHQFGDFKPLHPSVDAKLQSIC 240

Query: 241 QSLKQGET-------EKPPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCE 300
           QSLKQG+T       EKP + P PSESNS VNNF E ELK E ETS SSSS  SSPS  E
Sbjct: 241 QSLKQGKTEVCSVEDEKPTTIPLPSESNSAVNNFWEPELKSEVETSFSSSS--SSPSRSE 300

Query: 301 DF--STGSSPESSISFLDFSDSQWGEG-EAFCLEKYPSVEIDWAALNQLGESEC 332
           D+  STGSSP+SSISFLDFSDSQWGEG EAFCLEK+PS+EIDWAAL+QL ESEC
Sbjct: 301 DYSTSTGSSPDSSISFLDFSDSQWGEGVEAFCLEKFPSIEIDWAALSQLAESEC 349

[Close]

BLAST of Carg16003-RA vs. ExPASy TrEMBL
Match: A0A6J1GDE5 (ethylene-responsive transcription factor ERF060-like OS=Cucurbita moschata OX=3662 GN=LOC111453173 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 7.2e-131
Identity = 261/344 (75.87%), Postives = 282/344 (81.98%), Query Frame = 0


Query: 1   MAAALDFCTGISAPVYSSDPFCEELMKALQPFMKSAVST-----SSSSSVSYQPNVNPDF 60
           MAAA+D  TGIS  VYSSDPF EELMKA QPFMKSAVST     SSSSSVS QP ++   
Sbjct: 1   MAAAIDVYTGISTQVYSSDPFSEELMKAFQPFMKSAVSTSYSSSSSSSSVSCQPRLSTAV 60

Query: 61  CSPSSIRLFPQACSGIEQMGLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSF 120
           CSPSS  LF Q  SGIEQMGLEQSGPIGLNN TPSQ+ QIQAQIQLP QTMSS  SS   
Sbjct: 61  CSPSSTCLFSQGFSGIEQMGLEQSGPIGLNNLTPSQICQIQAQIQLPPQTMSSLSSS--- 120

Query: 121 RSRVSSQYHSVLAPKSISMKHTGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGT 180
            S   SQY + LAPKSI MK  GSP+KPT++YRG+RQRHWGKWVAEIRLPKNRTRLWLGT
Sbjct: 121 SSSFQSQYCNFLAPKSIPMKLIGSPSKPTKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGT 180

Query: 181 FDTAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGET 240
           FDT+EEAALAYDKAAYKLRG+ ARLNFPHLKHQFG+F+PLH SVDAKLQAIC+SLKQG+T
Sbjct: 181 FDTSEEAALAYDKAAYKLRGEFARLNFPHLKHQFGEFKPLHPSVDAKLQAICESLKQGKT 240

Query: 241 EKPPSA--------PPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSS 300
           EK  SA        PPP ESN VV+N+ E ELK E ETSSSSSSS SSPS  E+ STGSS
Sbjct: 241 EKLCSAEDEKANTSPPPLESNLVVDNYWEPELKSEVETSSSSSSSSSSPSRSEELSTGSS 300

Query: 301 PESSISFLDFSDSQWGEGEAFCLEKYPSVEIDWAALNQLGESEC 332
           PESSISFLDFSDSQWGEGEAFCLEK+PSVEIDWAAL+Q+ E EC
Sbjct: 301 PESSISFLDFSDSQWGEGEAFCLEKFPSVEIDWAALSQMVEWEC 341

[Close]

BLAST of Carg16003-RA vs. TAIR 10
Match: AT1G78080.1 (related to AP2 4 )

HSP 1 Score: 224.9 bits (572), Expect = 9.4e-59
Identity = 152/327 (46.48%), Postives = 201/327 (61.47%), Query Frame = 0


Query: 24  ELMKALQPFMKS-AVSTSSSSSVSYQPNVNPD-FCSPSSIRLFPQACSGIEQMG----LE 83
           ELM AL PF+KS + S SSSS+ S    ++P  F  P     +P +    +       L+
Sbjct: 20  ELMDALVPFIKSVSDSPSSSSAASASAFLHPSAFSLPPLPGYYPDSTFLTQPFSYGSDLQ 79

Query: 84  QSGP-IGLNNPTPSQVLQIQAQIQLP-SQTMSSFPSSFSFRSRVSSQYHSVLAPKSISMK 143
           Q+G  IGLNN + SQ+ QIQ+QI  P   T  +  +SFS          ++L+PK + MK
Sbjct: 80  QTGSLIGLNNLSSSQIHQIQSQIHHPLPPTHHNNNNSFS----------NLLSPKPLLMK 139

Query: 144 H-----------TGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAL 203
                       +G P+KPT++YRG+RQRHWGKWVAEIRLP+NRTRLWLGTFDTAEEAAL
Sbjct: 140 QSGVAGSCFAYGSGVPSKPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAAL 199

Query: 204 AYDKAAYKLRGDLARLNFPHLKH-------QFGDFEPLHSSVDAKLQAICQSLKQGETEK 263
           AYDKAAYKLRGD ARLNFP+L+H        FG+++PLHSSVDAKL+AIC+S+   ET+K
Sbjct: 200 AYDKAAYKLRGDFARLNFPNLRHNGSHIGGDFGEYKPLHSSVDAKLEAICKSM--AETQK 259

Query: 264 PPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDF 323
              +   S+      +  +   K++AE +S S       +  E+ + GSSP S ++F D 
Sbjct: 260 QDKSTKSSKKREKKVSSPDLSEKVKAEENSVSIGGSPPVTEFEESTAGSSPLSDLTFADP 319

[Close]

BLAST of Carg16003-RA vs. TAIR 10
Match: AT4G39780.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-55
Identity = 149/325 (45.85%), Postives = 187/325 (57.54%), Query Frame = 0


Query: 17  SSDPFCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSSIRLFPQACSGIEQMGL 76
           ++DPF EELMKALQP+     + + SSS +Y   V                       G 
Sbjct: 10  NTDPFQEELMKALQPY----TTNTDSSSPTYSNTV----------------------FGF 69

Query: 77  EQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVSSQYHSVLAPKSISMKH 136
            Q+  +GLN  TP Q+ QIQ Q+      +S  P+               LAPK + MK+
Sbjct: 70  NQTTSLGLNQLTPYQIHQIQNQLNQRRNIIS--PN---------------LAPKPVPMKN 129

Query: 137 TGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKLRGD 196
             +     ++YRG+RQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAA+AYD AAYKLRG+
Sbjct: 130 MTA----QKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAMAYDLAAYKLRGE 189

Query: 197 LARLNFPHLKHQFG------DFEPLHSSVDAKLQAICQSLKQGETEKPPSA-----PPPS 256
            ARLNFP  +H+ G       F PLHSSVDAKLQ ICQSL++ E    P +     PP +
Sbjct: 190 FARLNFPQFRHEDGYYGGGSCFNPLHSSVDAKLQEICQSLRKTEDIDLPCSETELFPPKT 249

Query: 257 ESNSVVNNFKEAELKIEAETSSSSSSSWSSPSLCEDFSTGSSPESSI-SFLDFSDSQWGE 316
           E       ++E+E         S  +S+S  S  E     SSPES I +FLDFSDS + E
Sbjct: 250 E-------YQESEYGF----LRSDENSFSDESHVE----SSSPESGITTFLDFSDSGFDE 272

Query: 317 GEAFCLEKYPSVEIDWAALNQLGES 330
             +F LEK+PSVEIDW A+++L ES
Sbjct: 310 IGSFGLEKFPSVEIDWDAISKLSES 272

[Close]

BLAST of Carg16003-RA vs. TAIR 10
Match: AT5G65130.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.5e-51
Identity = 135/309 (43.69%), Postives = 186/309 (60.19%), Query Frame = 0


Query: 21  FCEELMKALQPFMKSAVSTSSSSSVSYQPNVNPDFCSPSSIRLFPQACSGIEQMGLEQSG 80
           + +E M+AL+PFMK   S+S+S+S + +P + P+F  P++ ++ P +          Q+G
Sbjct: 8   YVDEFMEALEPFMKVTSSSSTSNSSNPKP-LTPNFI-PNNDQVLPVS---------NQTG 67

Query: 81  PIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVSSQYHSVLAPKSISMKHTGSP 140
           PIGLN  TP+Q+LQIQ ++ L  Q  S        R R  S    +L  K  SMK     
Sbjct: 68  PIGLNQLTPTQILQIQTELHL-RQNQS--------RRRAGSH---LLTAKPTSMKKIDVA 127

Query: 141 TKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKLRGDLARL 200
           TKP ++YRG+RQR WGKWVAEIRLPKNRTRLWLGTF+TA+EAALAYD+AA+K+RGD ARL
Sbjct: 128 TKPVKLYRGVRQRQWGKWVAEIRLPKNRTRLWLGTFETAQEAALAYDQAAHKIRGDNARL 187

Query: 201 NFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKP-PSAPPPSESNSVVNNFKEAEL 260
           NFP +  Q    + L  S++AK+++IC S     ++ P P     +++  V++ F + E 
Sbjct: 188 NFPDIVRQGHYKQILSPSINAKIESICNS-----SDLPLPQIEKQNKTEEVLSGFSKPEK 247

Query: 261 KIEAETSSSSSSSWSSPSLCEDFSTGSSPESSISFLDFSDSQWGEGEAFC--LEKYPSVE 320
             E E        +S          GSSPES I+ LDFS     E E+F   L KYPS+E
Sbjct: 248 --EPEFGEIYGCGYS----------GSSPESDITLLDFSSDCVKEDESFLMGLHKYPSLE 276

Query: 321 IDWAALNQL 327
           IDW A+ +L
Sbjct: 308 IDWDAIEKL 276

[Close]

BLAST of Carg16003-RA vs. TAIR 10
Match: AT1G22190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 4.7e-50
Identity = 125/237 (52.74%), Postives = 155/237 (65.40%), Query Frame = 0


Query: 99  IQLPSQTMSSF----PSSFSFRSRVSSQYHSVLAPKSISMKHTG-SPTKPTRVYRGIRQR 158
           I+ PS   S+F    P+  S+ S + S  H  L+PK +SMK TG S  KPT++YRG+RQR
Sbjct: 31  IKSPSNDSSAFAFSLPAPISYGSDLHSFSHH-LSPKPVSMKQTGTSAAKPTKLYRGVRQR 90

Query: 159 HWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKLRGDLARLNFPHLKHQFGDFE 218
           HWGKWVAEIRLP+NRTRLWLGTFDTAEEAALAYDKAAYKLRGD ARLNFP L+H   +++
Sbjct: 91  HWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPDLRHN-DEYQ 150

Query: 219 PLHSSVDAKLQAICQSLKQGETEKPPSAPPPSESNSVVNNFKEAELKIEAETSSSSSSSW 278
           PL SSVDAKL+AICQ+L +  T+K   +   S S           +K+  E  SS+    
Sbjct: 151 PLQSSVDAKLEAICQNLAE-TTQKQVRSTKKSSSR---KRSSTVAVKLPEEDYSSAG--- 210

Query: 279 SSPSLCEDFSTG--SSPESSISFLDFSDS---QWGEGEAFCLEKYPSVEIDWAALNQ 326
           SSP L E + +G  SSP S ++F D  +     W E     LEKYPS EIDW ++ Q
Sbjct: 211 SSPLLTESYGSGGSSSPLSELTFGDTEEEIQPPWNEN---ALEKYPSYEIDWDSILQ 255

[Close]

BLAST of Carg16003-RA vs. TAIR 10
Match: AT2G22200.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-48
Identity = 124/268 (46.27%), Postives = 166/268 (61.94%), Query Frame = 0


Query: 75  GLEQSGPIGLNNPTPSQVLQIQAQIQLPSQTMSSFPSSFSFRSRVSSQYHSVLAPKSISM 134
           G+ +S P+GLN  TP Q+ QIQ Q+     T+S+                  L+P  I M
Sbjct: 16  GVNKSMPLGLNQLTPYQIHQIQNQLNHRRSTISN------------------LSPNRIRM 75

Query: 135 KH-TGSPTKPTRVYRGIRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAALAYDKAAYKL 194
           K+ T S +K   +YRG+RQRHWGKWVAEIRLPKNRTRLWLGTF+TAE+AALAYD+AA++L
Sbjct: 76  KNLTPSTSKTKNLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFETAEKAALAYDQAAFQL 135

Query: 195 RGDLARLNFPHLKHQFGDFEPLHSSVDAKLQAICQSLKQGETEKPPSAPPPSESNSVVNN 254
           RGD+A+LNFP+L H+  D  PL SSVD KLQAIC+SL+  +TE+  S    ++  SV + 
Sbjct: 136 RGDIAKLNFPNLIHE--DMNPLPSSVDTKLQAICKSLR--KTEEICSVSDQTKEYSVYSV 195

Query: 255 FKEAEL-----------KIEAETSS-SSSSSWSSPSLCEDFSTGSSPESSISFLDFSDSQ 314
             + EL           +   ET+  S+ S  S  +   D S      S  +FLDFSD++
Sbjct: 196 SDKTELFLPKAELFLPKREHLETNELSNESPRSDETSLLDESQAEYSSSDKTFLDFSDTE 255

Query: 315 WGEGEAFCLEKYPSVEIDWAALNQLGES 330
           + E  +F L K+PSVEIDW A+++L  S
Sbjct: 256 FEEIGSFGLRKFPSVEIDWDAISKLANS 261

The following BLAST results are available for this feature:

BLAST of Carg16003-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026835.1	1.3e-187	100.00	Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp....	[more]
KAG6594870.1	1.3e-182	97.65	Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp....	[more]
XP_023517236.1	3.5e-180	97.02	ethylene-responsive transcription factor RAP2-13-like [Cucurbita pepo subsp. pep...	[more]
XP_022963399.1	4.5e-180	98.49	ethylene-responsive transcription factor ERF060-like [Cucurbita moschata]	[more]
XP_023004027.1	1.2e-175	94.19	ethylene-responsive transcription factor ERF060-like [Cucurbita maxima]	[more]

BLAST of Carg16003-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H1E4	1.3e-57	46.48	Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana OX=3702 ...	[more]
O65665	2.3e-54	45.85	Ethylene-responsive transcription factor ERF060 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9FJQ2	3.5e-50	43.69	Ethylene-responsive transcription factor ERF057 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LM15	6.6e-49	52.74	Ethylene-responsive transcription factor RAP2-13 OS=Arabidopsis thaliana OX=3702...	[more]
Q9SIE4	1.6e-47	46.27	Ethylene-responsive transcription factor ERF056 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg16003-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HK00	2.2e-180	98.49	ethylene-responsive transcription factor ERF060-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1KP93	5.6e-176	94.19	ethylene-responsive transcription factor ERF060-like OS=Cucurbita maxima OX=3661...	[more]
A0A5A7T4I4	1.4e-134	75.42	Ethylene-responsive transcription factor ERF060 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3B297	1.4e-134	75.42	ethylene-responsive transcription factor ERF060 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1GDE5	7.2e-131	75.87	ethylene-responsive transcription factor ERF060-like OS=Cucurbita moschata OX=36...	[more]

BLAST of Carg16003-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78080.1	9.4e-59	46.48	related to AP2 4	[more]
AT4G39780.1	1.7e-55	45.85	Integrase-type DNA-binding superfamily protein	[more]
AT5G65130.1	2.5e-51	43.69	Integrase-type DNA-binding superfamily protein	[more]
AT1G22190.1	4.7e-50	52.74	Integrase-type DNA-binding superfamily protein	[more]
AT2G22200.1	1.2e-48	46.27	Integrase-type DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 169..185 score: 71.66 coord: 147..158 score: 66.52
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 146..209 e-value: 1.0E-36 score: 138.0
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 146..195 e-value: 3.4E-14 score: 52.8
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 146..203 score: 23.050652
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 147..205 e-value: 2.09816E-34 score: 118.528
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 146..204 e-value: 2.1E-26 score: 93.8
None	No IPR available	PANTHER	PTHR31657	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061	coord: 1..325
None	No IPR available	PANTHER	PTHR31657:SF45	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF060-LIKE	coord: 1..325
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 146..204

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg16003	Carg16003	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg16003-RA:1	Carg16003-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg16003-RA:cds	Carg16003-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg16003-RA	Carg16003-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity