Carg15249-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15249-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nicotianamine synthase-like
Location	Carg_Chr10: 1902024 .. 1902881 (+)
Sequence length	858
RNA-Seq Expression	Carg15249-RA
Synteny	Carg15249-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGCCCTCCACATTCTTGGCACCGAAACCGATATCTCGGAGGAGTTTTTGATAGCTCGAGTCATCGACCTCCATGGAAGCATCTCGAGGCTTGAAACCCTAAAGCCATGCAAGGAAGTGAACGGCCTCTTCACCAACCTAGTGAAATTGTGCATTTTGCCTTGTTCAATAGATGTCTCTACATTGGCACCTCATTTACAAGCCATTCGTGAGAGCCTCATACTTTTATGTGGCCGAGCCGAGGGGTTACTCGAGTTAGAATTCTCGACCATGCTCGGAAAAATCCCGAAACCTCTCAACAATCTTACTCTATTTCCATATTATGACAACTACATCAAATTAGCCCACCTTGAATACAAGATATTGATTGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGGTCCGGTCCTCTTCCTCTAACTTCCATTATATTGGCCATGCAGCACATGAAGAACACGTATTTCGACAACTACGACGTCGATAGCATGGCAAATGAGGTGGCGGGAAAGATCGTGGCTTCGGACGGTGAGTTGGAGGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAGGAGAAGAGCTTGAGGGATACGATTGTGTGTTCTTGGCAGCTCTTGTGGGGTTGAACAAGGAAGATAAAGTGAAGATTTTAAGTCACTTGAGAAAGTATATGAAACAAGGTGGGGTTTTGGTTGTGAGAAGTGCTAGAGGAGGTCGAGCCTTTTTGTACCCCGTGGTTGAGGTCGCCGATATGGTTGGCTTTGACATTCTCTCTATTTTTCATCCCTCCGACGACGTCGTCAATTCCGTTCTCTTGGCTCGCAAGCCTATACTCCTATGA

mRNA sequence

ATGACAGCCCTCCACATTCTTGGCACCGAAACCGATATCTCGGAGGAGTTTTTGATAGCTCGAGTCATCGACCTCCATGGAAGCATCTCGAGGCTTGAAACCCTAAAGCCATGCAAGGAAGTGAACGGCCTCTTCACCAACCTAGTGAAATTGTGCATTTTGCCTTGTTCAATAGATGTCTCTACATTGGCACCTCATTTACAAGCCATTCGTGAGAGCCTCATACTTTTATGTGGCCGAGCCGAGGGGTTACTCGAGTTAGAATTCTCGACCATGCTCGGAAAAATCCCGAAACCTCTCAACAATCTTACTCTATTTCCATATTATGACAACTACATCAAATTAGCCCACCTTGAATACAAGATATTGATTGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGGTCCGGTCCTCTTCCTCTAACTTCCATTATATTGGCCATGCAGCACATGAAGAACACGTATTTCGACAACTACGACGTCGATAGCATGGCAAATGAGGTGGCGGGAAAGATCGTGGCTTCGGACGGTGAGTTGGAGGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAGGAGAAGAGCTTGAGGGATACGATTGTGTGTTCTTGGCAGCTCTTGTGGGGTTGAACAAGGAAGATAAAGTGAAGATTTTAAGTCACTTGAGAAAGTATATGAAACAAGGTGGGGTTTTGGTTGTGAGAAGTGCTAGAGGAGGTCGAGCCTTTTTGTACCCCGTGGTTGAGGTCGCCGATATGGTTGGCTTTGACATTCTCTCTATTTTTCATCCCTCCGACGACGTCGTCAATTCCGTTCTCTTGGCTCGCAAGCCTATACTCCTATGA

Coding sequence (CDS)

ATGACAGCCCTCCACATTCTTGGCACCGAAACCGATATCTCGGAGGAGTTTTTGATAGCTCGAGTCATCGACCTCCATGGAAGCATCTCGAGGCTTGAAACCCTAAAGCCATGCAAGGAAGTGAACGGCCTCTTCACCAACCTAGTGAAATTGTGCATTTTGCCTTGTTCAATAGATGTCTCTACATTGGCACCTCATTTACAAGCCATTCGTGAGAGCCTCATACTTTTATGTGGCCGAGCCGAGGGGTTACTCGAGTTAGAATTCTCGACCATGCTCGGAAAAATCCCGAAACCTCTCAACAATCTTACTCTATTTCCATATTATGACAACTACATCAAATTAGCCCACCTTGAATACAAGATATTGATTGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGGTCCGGTCCTCTTCCTCTAACTTCCATTATATTGGCCATGCAGCACATGAAGAACACGTATTTCGACAACTACGACGTCGATAGCATGGCAAATGAGGTGGCGGGAAAGATCGTGGCTTCGGACGGTGAGTTGGAGGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAGGAGAAGAGCTTGAGGGATACGATTGTGTGTTCTTGGCAGCTCTTGTGGGGTTGAACAAGGAAGATAAAGTGAAGATTTTAAGTCACTTGAGAAAGTATATGAAACAAGGTGGGGTTTTGGTTGTGAGAAGTGCTAGAGGAGGTCGAGCCTTTTTGTACCCCGTGGTTGAGGTCGCCGATATGGTTGGCTTTGACATTCTCTCTATTTTTCATCCCTCCGACGACGTCGTCAATTCCGTTCTCTTGGCTCGCAAGCCTATACTCCTATGA

Protein sequence

MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDVSTLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL

Homology

[Close]

BLAST of Carg15249-RA vs. NCBI nr
Match: KAG7023395.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.7 bits (1454), Expect = 4.6e-157
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV
Sbjct: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 286
           ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Carg15249-RA vs. NCBI nr
Match: KAG6589715.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 559.7 bits (1441), Expect = 1.5e-155
Identity = 283/285 (99.30%), Postives = 283/285 (99.30%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M AL ILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV
Sbjct: 1   MAALQILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 286
           ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Carg15249-RA vs. NCBI nr
Match: XP_022922054.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 557.0 bits (1434), Expect = 9.5e-155
Identity = 281/285 (98.60%), Postives = 283/285 (99.30%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M ALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFT+LVKLCILPCSIDV
Sbjct: 1   MAALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTSLVKLCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYY NYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYGNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 286
           A+GGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Carg15249-RA vs. NCBI nr
Match: XP_022987937.1 (nicotianamine synthase-like [Cucurbita maxima])

HSP 1 Score: 547.0 bits (1408), Expect = 9.8e-152
Identity = 275/285 (96.49%), Postives = 278/285 (97.54%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M AL ILGTETDISEE LIARVIDLHGSISRLETLKPCKEVNGLFTNLVK CILPCSIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL PHLQAIRESLIL CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYD+DSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGL KEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 286
           A+GGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPIL+
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of Carg15249-RA vs. NCBI nr
Match: XP_023516742.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo] >XP_023521836.1 nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 539.3 bits (1388), Expect = 2.0e-149
Identity = 268/285 (94.04%), Postives = 276/285 (96.84%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M A  ILGTETDISEEFLIAR+IDLHGSISRLETLKPCKEVNGLFT LVK CILPCSIDV
Sbjct: 1   MAAHQILGTETDISEEFLIARIIDLHGSISRLETLKPCKEVNGLFTKLVKFCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           +TL PHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  TTLPPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KILIDNG+VNPK+VAFVGSGPLPLTSIILAMQHMKNTYFDNYD+DSMANEVAGKIVASDG
Sbjct: 121 KILIDNGIVNPKRVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGL KEDKVKILSHLRKYMKQGG LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGALVVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 286
           A+GGRAFLYPVV+VADM GFDILSIFHPSDDVVNSVLLARKPIL+
Sbjct: 241 AKGGRAFLYPVVDVADMAGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of Carg15249-RA vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.8e-79
Identity = 145/264 (54.92%), Postives = 194/264 (73.48%), Query Frame = 0


Query: 18  LIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDVSTLAPHLQAIRESLILL 77
           ++ +V +L+  ISRLE L P K+VN LFT+LV  C+ P  IDVS L   +Q IR  LI L
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNPIDVSKLCQKIQEIRSHLIKL 68

Query: 78  CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKKVAFV 137
           CG+AEGLLE  FS +L     PL +L +FPY+DNYIKL+ LEY IL  N    PKK+AF+
Sbjct: 69  CGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIAFI 128

Query: 138 GSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWTRDIVEVG 197
           GSGPLPLTS++LA +H+K T F NYD+D  AN +A  +VA+D ++  RM F T D+++V 
Sbjct: 129 GSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVT 188

Query: 198 EELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYPVVEVADM 257
             L+ YD VFLAALVG++KEDKVK++ HL KYM  G  L++RSA G RAFLYPV++  D+
Sbjct: 189 CALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDL 248

Query: 258 VGFDILSIFHPSDDVVNSVLLARK 282
            GF++LS++HP+D+V+NSV++ARK
Sbjct: 249 RGFEVLSVYHPTDEVINSVIIARK 272

[Close]

BLAST of Carg15249-RA vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 4.5e-75
Identity = 138/271 (50.92%), Postives = 206/271 (76.01%), Query Frame = 0


Query: 15  EEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILP-CSIDVSTLAPHLQAIRES 74
           ++ L+ ++ DL+  IS+LETLKPC++V+ LF  LV  CI P  +IDV+ ++ ++Q +R +
Sbjct: 6   DDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSN 65

Query: 75  LILLCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI +CG AEG LE  FS++L      PL++L LFPYY+NY+KL+ LE+ +L    NG V 
Sbjct: 66  LIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV- 125

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWT 194
           P+ VAF+GSGPLPLTS++LA  H+K++ F N+D+D  AN VA ++V+SD +L  RM F T
Sbjct: 126 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 185

Query: 195 RDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYP 254
            DI++V E L+G+D VFLAALVG++K++KVK++ HL K+M  G +L++RSA G RAFLYP
Sbjct: 186 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 245

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LS++HP+D+V+NS++++RK
Sbjct: 246 IVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Carg15249-RA vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-73
Identity = 143/271 (52.77%), Postives = 200/271 (73.80%), Query Frame = 0


Query: 15  EEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILP-CSIDVSTLAPHLQAIRES 74
           +E L+  + DL+  IS+LE+LKP ++VN LF  LV  CI P  +IDV+ +   +Q IR +
Sbjct: 5   DEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLN 64

Query: 75  LILLCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI +CG AEG LE  FS++L      PL++L +FPYY+NY+KL  LE+ +L    NG V 
Sbjct: 65  LIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV- 124

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWT 194
           PK VAF+GSGPLPLTSI+LA  H+K+T F N+D+D  AN +A  +V+SD ++  RM F T
Sbjct: 125 PKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHT 184

Query: 195 RDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYP 254
            DI++V E L+ +D VFLAALVG+NKE+KVK++ HL+K+M  G VL++RSA G RAFLYP
Sbjct: 185 VDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYP 244

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LSI+HP+DDV+NSV++++K
Sbjct: 245 IVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of Carg15249-RA vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.0e-71
Identity = 135/268 (50.37%), Postives = 191/268 (71.27%), Query Frame = 0


Query: 18  LIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCI-LPCSIDVSTLAPHLQAIRESLIL 77
           ++ ++IDL+  IS+L++LKP K V+ LF  LV  C+    +IDV+ +   ++ +R +LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGRAEGLLELEFSTMLGKIPK---PLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKK 137
           LCG AEG LE  FST+LG + +   PL++L +FPYY NY+KL  LE+ +L  +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWTRDI 197
           +AFVGSGP+PLTSI+LA  H+ NT F N+D+DS AN +A  +V+ D +L  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYPVVE 257
           +   E L+ YD VFLAALVG++KE KVK + HL K+M  G VL++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VADMVGFDILSIFHPSDDVVNSVLLARK 282
            +D+ GF +L+I+HP+DDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Carg15249-RA vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.2e-70
Identity = 136/268 (50.75%), Postives = 186/268 (69.40%), Query Frame = 0


Query: 18  LIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDVSTLAPH---LQAIRESL 77
           ++ +++DL+  IS LE+LKP K V+ LF  LV  C LP   ++     H   ++ +R  L
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTC-LPTDTNIDVTEIHDEKVKDMRSHL 67

Query: 78  ILLCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKK 137
           I LCG AEG LE  FS +LG     PLN+L +FPYY+NY+KL  LE+ +L  +    P K
Sbjct: 68  IKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTK 127

Query: 138 VAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWTRDI 197
           VAF+GSGP+PLTSI+LA  H+ NT F N+D+DS AN +A  +V+ D +L  RM F T D+
Sbjct: 128 VAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDV 187

Query: 198 VEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYPVVE 257
           +   E L+ YD VFLAALVG++KE KVK + HL K+M  G V+++RSA G RAFLYP+V+
Sbjct: 188 LNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVD 247

Query: 258 VADMVGFDILSIFHPSDDVVNSVLLARK 282
             D+ GF++L+I+HPSDDVVNSV++ARK
Sbjct: 248 SCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Carg15249-RA vs. ExPASy TrEMBL
Match: A0A6J1E292 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.6e-155
Identity = 281/285 (98.60%), Postives = 283/285 (99.30%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M ALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFT+LVKLCILPCSIDV
Sbjct: 1   MAALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTSLVKLCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYY NYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYGNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 286
           A+GGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Carg15249-RA vs. ExPASy TrEMBL
Match: A0A6J1JBQ8 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 4.8e-152
Identity = 275/285 (96.49%), Postives = 278/285 (97.54%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M AL ILGTETDISEE LIARVIDLHGSISRLETLKPCKEVNGLFTNLVK CILPCSIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL PHLQAIRESLIL CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYD+DSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGL KEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 286
           A+GGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPIL+
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of Carg15249-RA vs. ExPASy TrEMBL
Match: A0A5D3CIT2 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00680 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.1e-124
Identity = 225/281 (80.07%), Postives = 253/281 (90.04%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M AL   G E +IS  FL+ R+IDLH SI +LETL+PCK+VN LFTNLV LCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL P+LQAIRESLI+LCG+AEGLLELEFST+L KIPKPLNNLTLFPYY NY+KLA+LE 
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDVDS+AN+VA KIV SD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKF + DIVEV EEL GYDCVFLAALVG+NKE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           A+GGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+LARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Carg15249-RA vs. ExPASy TrEMBL
Match: A0A1S3BXH0 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.1e-124
Identity = 225/281 (80.07%), Postives = 253/281 (90.04%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M AL   G E +IS  FL+ R+IDLH SI +LETL+PCK+VN LFTNLV LCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL P+LQAIRESLI+LCG+AEGLLELEFST+L KIPKPLNNLTLFPYY NY+KLA+LE 
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDVDS+AN+VA KIV SD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKF + DIVEV EEL GYDCVFLAALVG+NKE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           A+GGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+LARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Carg15249-RA vs. ExPASy TrEMBL
Match: A0A0A0LUJ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.9e-124
Identity = 224/281 (79.72%), Postives = 254/281 (90.39%), Query Frame = 0


Query: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60
           M AL I GTE +IS  FL+ R+IDLH SIS+LETL+PCK+VN LFTNLV LCILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL P+LQ IRESLI+LCG+AEGLLELEFST+L KIPKPLNNLTLFPYY NYIKLA+LE 
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK T+FDNYDVDS+AN+VA KIV SD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240
           +LEGRMKF + DIV+V EEL GYDCVFLAALVG+NKE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           A+GGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+L RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of Carg15249-RA vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 282.7 bits (722), Expect = 3.2e-76
Identity = 138/271 (50.92%), Postives = 206/271 (76.01%), Query Frame = 0


Query: 15  EEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILP-CSIDVSTLAPHLQAIRES 74
           ++ L+ ++ DL+  IS+LETLKPC++V+ LF  LV  CI P  +IDV+ ++ ++Q +R +
Sbjct: 6   DDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSN 65

Query: 75  LILLCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI +CG AEG LE  FS++L      PL++L LFPYY+NY+KL+ LE+ +L    NG V 
Sbjct: 66  LIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV- 125

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWT 194
           P+ VAF+GSGPLPLTS++LA  H+K++ F N+D+D  AN VA ++V+SD +L  RM F T
Sbjct: 126 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 185

Query: 195 RDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYP 254
            DI++V E L+G+D VFLAALVG++K++KVK++ HL K+M  G +L++RSA G RAFLYP
Sbjct: 186 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 245

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LS++HP+D+V+NS++++RK
Sbjct: 246 IVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Carg15249-RA vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 278.1 bits (710), Expect = 7.9e-75
Identity = 143/271 (52.77%), Postives = 200/271 (73.80%), Query Frame = 0


Query: 15  EEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILP-CSIDVSTLAPHLQAIRES 74
           +E L+  + DL+  IS+LE+LKP ++VN LF  LV  CI P  +IDV+ +   +Q IR +
Sbjct: 5   DEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLN 64

Query: 75  LILLCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI +CG AEG LE  FS++L      PL++L +FPYY+NY+KL  LE+ +L    NG V 
Sbjct: 65  LIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV- 124

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWT 194
           PK VAF+GSGPLPLTSI+LA  H+K+T F N+D+D  AN +A  +V+SD ++  RM F T
Sbjct: 125 PKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHT 184

Query: 195 RDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYP 254
            DI++V E L+ +D VFLAALVG+NKE+KVK++ HL+K+M  G VL++RSA G RAFLYP
Sbjct: 185 VDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYP 244

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LSI+HP+DDV+NSV++++K
Sbjct: 245 IVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of Carg15249-RA vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 269.6 bits (688), Expect = 2.8e-72
Identity = 135/268 (50.37%), Postives = 191/268 (71.27%), Query Frame = 0


Query: 18  LIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCI-LPCSIDVSTLAPHLQAIRESLIL 77
           ++ ++IDL+  IS+L++LKP K V+ LF  LV  C+    +IDV+ +   ++ +R +LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGRAEGLLELEFSTMLGKIPK---PLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKK 137
           LCG AEG LE  FST+LG + +   PL++L +FPYY NY+KL  LE+ +L  +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWTRDI 197
           +AFVGSGP+PLTSI+LA  H+ NT F N+D+DS AN +A  +V+ D +L  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYPVVE 257
           +   E L+ YD VFLAALVG++KE KVK + HL K+M  G VL++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VADMVGFDILSIFHPSDDVVNSVLLARK 282
            +D+ GF +L+I+HP+DDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Carg15249-RA vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 268.1 bits (684), Expect = 8.2e-72
Identity = 136/268 (50.75%), Postives = 186/268 (69.40%), Query Frame = 0


Query: 18  LIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDVSTLAPH---LQAIRESL 77
           ++ +++DL+  IS LE+LKP K V+ LF  LV  C LP   ++     H   ++ +R  L
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTC-LPTDTNIDVTEIHDEKVKDMRSHL 67

Query: 78  ILLCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKK 137
           I LCG AEG LE  FS +LG     PLN+L +FPYY+NY+KL  LE+ +L  +    P K
Sbjct: 68  IKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTK 127

Query: 138 VAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDGELEGRMKFWTRDI 197
           VAF+GSGP+PLTSI+LA  H+ NT F N+D+DS AN +A  +V+ D +L  RM F T D+
Sbjct: 128 VAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDV 187

Query: 198 VEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRSARGGRAFLYPVVE 257
           +   E L+ YD VFLAALVG++KE KVK + HL K+M  G V+++RSA G RAFLYP+V+
Sbjct: 188 LNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVD 247

Query: 258 VADMVGFDILSIFHPSDDVVNSVLLARK 282
             D+ GF++L+I+HPSDDVVNSV++ARK
Sbjct: 248 SCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Carg15249-RA vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 57.4 bits (137), Expect = 2.2e-08
Identity = 29/54 (53.70%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 230 MKQGGVLVVRSARGGRAFLYPVVEVADMVGFDILSIFHP--SDDVVNSVLLARK 282
           M  G +L++RSARG R+FLY  V+  D+ GF++L I+HP  SD  VNSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:

BLAST of Carg15249-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023395.1	4.6e-157	100.00	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6589715.1	1.5e-155	99.30	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922054.1	9.5e-155	98.60	nicotianamine synthase-like [Cucurbita moschata]	[more]
XP_022987937.1	9.8e-152	96.49	nicotianamine synthase-like [Cucurbita maxima]	[more]
XP_023516742.1	2.0e-149	94.04	nicotianamine synthase-like [Cucurbita pepo subsp. pepo] >XP_023521836.1 nicotia...	[more]

BLAST of Carg15249-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	1.8e-79	54.92	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	4.5e-75	50.92	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	1.1e-73	52.77	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	4.0e-71	50.37	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	1.2e-70	50.75	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Carg15249-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E292	4.6e-155	98.60	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 S...	[more]
A0A6J1JBQ8	4.8e-152	96.49	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=...	[more]
A0A5D3CIT2	1.1e-124	80.07	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BXH0	1.1e-124	80.07	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1	[more]
A0A0A0LUJ7	1.9e-124	79.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1	[more]

BLAST of Carg15249-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	3.2e-76	50.92	nicotianamine synthase 4	[more]
AT1G09240.1	7.9e-75	52.77	nicotianamine synthase 3	[more]
AT5G04950.1	2.8e-72	50.37	nicotianamine synthase 1	[more]
AT5G56080.1	8.2e-72	50.75	nicotianamine synthase 2	[more]
AT4G26483.1	2.2e-08	53.70	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 15..282 e-value: 4.2E-104 score: 347.7
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..283
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 11..285 score: 48.35376
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 20..282 e-value: 4.4E-58 score: 198.5
None	No IPR available	PANTHER	PTHR32266:SF11	NICOTIANAMINE SYNTHASE	coord: 1..283
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 125..241

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg15249	Carg15249	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg15249-RA:1	Carg15249-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg15249-RA:cds	Carg15249-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg15249-RA	Carg15249-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity