Carg15159-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15159-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionLEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2
LocationCarg_Chr10: 1407109 .. 1410759 (-)
Sequence length1743
RNA-Seq ExpressionCarg15159-RA
SyntenyCarg15159-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACGTTTCTGCTGCGGATCCATTTTCGGAACGCCGCCGTGCTCGTCACCTACGCCACCGCCTTCCTCCTCCGCTTTCGGCCGTCTCTCGCCGACGATAACGACGACTGTAAGATAGGCACGTGTCCTTTTCTTTTACCTCCTCCGCCAGGTATGCTATTATACCTTCTAATTTACTGATTTATTATAGTAACTGTCAATCACCTCTTTCAGATATCGTTCTTTTTAGCCTTTTATGGTCAAATTGTCTTTTTCTCTAAAAAATTTTAAACGTCTCTGTTATGTCGAGGTTTTCACATCCAAATAATGTTTCATACACCTTGAGGTCCGGTATTCTGTCCAGTATCAGTTATCATTTGTAACAGCTAAAATTCACTTCGATTGTCCTTTTTGAGCGCCGGAAATTTTAAAATGCAGAGATTCTCACCTTGTATAACGAATGTTTGGTTTCTCTCCACTGACGTTCATCTCACATCATTATTGCTGATAATTAGATAATAAACGGTGTTTAGTTATTTATTAATATAATCTCACCTTCCTTACTTATTTCCATTGTGTTCTTGATTTGATTTTATTTTCATGTTGTTCTTATTTTGTTTTGAAACTTTGACCAAATTCAATGTGGAAGGACAAAATTTTCGCATAAATATATAATATGACATAACGTACCAAACGAAAATTTGGAGTTCAAACCTTCGAACTATCAAATTTGGACTAGTCAAAGTTTAGACTATATATATATTTCCCAACTGTTATACAAAAGTTAATAATTATTTTTGGTATTAGTGCAAAAAAATAATTCATACTACATTCATTAATTACTCATAAAAGAATTATTTAAAAATGATGTGGACTATAAAATTAAATAAATATATTATTTAATAGGTACTGACATGGCACATGTTTATGTCATAGTAAGGTTTCGAGAAGTGTCTCGAACGACTTTTTTTAAAATGAGTTTTTCAAGGATAGTACTGGATAGCATGGCTGTGCCGGTATTACCCCAACCCACGTGTTGGAGGTTGGATTTTGTACCCGTTATCCAGTACGGTATTTGTAAGTGACATGACATGGACACGGGAGAGCCAATGGCTCGATGAAATTTCGATGGATGATATTCAGAATGTCCCAATGAGATAAATGGCATGTGGGTCCGTTCTCAATAGCATGACCAAGTGAATTGTGATGACCAAAGACTCCCTAGGAGCGTCAAGACTTAACATTTTTCTTTCGATTGCATTTTCTTTCTTCTTCCACTAATCAAACCTTCCCACATGGAAAACGTTGAAGTCTTCCTGTACCAAATCTTCAACATTCATAATGACAAATTTGCCCCTATCTTGTGCACAAAGAAAATGCTTAATATGAACTGATGAACAAATATTGGGATTCTTTAGCAGTGATTTTTTTTCGAAGTTTTTCATGTTTCTCTGTTATGTTTTAATCCGACATTGATGTTCTTTTTCTTCATTGTTTACGTTGCAGACAATAGCGACGACATCAAAAAGAAGATTATCATTGGTAACTCTGTTTTTCCTCCTCTGTTCATATGGTTGCCTCTATGGCAATTGTTTAAAATCTCCATTGGTTTGATTCATATGCAGGCACATGCACTGGTTTTGGAGCCTTGCTTTTAACCTTACTTGTTCTTGGCATCTGCTACCGATGGCGCCAGCTCCGTCGTAAAAGGTCTCACATGCTGCCATACGTGCAACGGAGCGTTCTCTCGAATCCCCCGTCCGTGGAAGAAGCTGAGAATGGAGGTACCTACTTGGGCGTTCACCTCTTCTCCTATAAAGAACTTGAAGAAGCCACGAACCATTTCGACTCCAACAAAGAGCTCGGCGATGGAGGCTTTGGCACAGTGCATTATGGTAAGAGTTGTTAGGAATCACGGATCTTTACAATGGTATGATATTATCCACTTTGACCTCGTACCAATGGAGATGTATTCCTTACCTATAAACCCGTGATCATTCCCTAAATTAGCCCATGCAGGACCCATAGCAGCCCTTCGAATCTAATCTAATCATTGTTCATGCTTGATTATTAGCTAATGATGGTTTCATTGATCTTTTTGTTCATATATCTGTTTAGGCTTACTCAAAGACGGGCGTGCCGTTGCTGTCAAACGGTTGTTCGAAAGCAATTTCAAGAGAGTTGAGCAGTTCATGAATGAGGTCGGGATCCTCGCACGCTTACGCCACCACAACCTCGTTTCACTTTACGGTTGTACCTCGAGCAACAGCCGCGAGCTTCTGCTCGTGTATGAATACGTTCCGAACGGTACGGTGGCTGATCACCTCCATGGAAAACTAGCAAAGCCAAGCAAGCTTCCATGGTCTACAAGAATGAAGATTGCTATAGAGACTGCAAGTGCTTTGGTGTATCTCCATGCTTCTGAAATCATCCACCGTGATGTCAAAACCAACAACATCCTCCTCGACAACCACTACTCCGTTAAAGTAGCCGATTTCGGGTTATCCCGCCTCTTCCCACTCGACGTCACGCACGTTTCAACCGCTCCACAAGGTACTCCCGGCTACGTCGACCCGGAGTACCATCAGTGCTACCAACTCTCTGATAAAAGCGATGTCTTCAGCTTCGGCGTAGTACTTGTTGAACTCATGTCCTCAATGCCTGCTGTTGACATCACAAGACATAGACAAGAGATCAACCTGTTCAACATGGCCATCAACAAGATCCAAAGCCACGCATTACACGAACTCGTCGACCCATCGCTCGGTTTCGAATCAGACGACAAAATCAAAGACATGATAACGAGCGTCGCCGAGGTTGCATTTCAATGCCTGCAGAGCATGAAAGACGAGAGGCCAACAATGATGGAAGTTCTCGACAGTCTAAACGCCATACAGAATCGAAACGGTGATGCTGTGCAGGTAAAGAATGGCTATGAATCTTCACCAAGTTCCATGTCAGTGTCTTGGGTTAGTAGTAACACCTCTACAGCAACAAATTATTGAATTGATTCTTCATTCTTTGTCTTAGAACACTACATTATATGTCATTTATACCAAAAAAAGGAACATAAAGGGAAGATTTTTACAATAACCCATGTTATCAAATTACATATAAATGAATCGTATAAGCTCAGAAAATCAAAAGGAATTGGTTTATGCACATAATTTAATGATTAAACTCCATGAGCTTAGGAGCAAACAAGAGGAGCCTATTCAAGGAATTGGGCATGAATTTCCTGGCAAACAAATGGCATACATTGGTGTCAGCTCCATTGTACCGGCAGTGGCTGCCAGTCCTCAGTCTTTGGAGCAAGCCAACGTTGACATCCATTCTGTTGAACTTGGTGGGGTGGGCGCCGCCTTTGGACCAGTCGACCCAAGTCAAGGTCCGGTTGGAATTGGTGGTGGAGAAGTGGATTCCGACGAGGGTGGGGAGGTAGTGCTCGTCCATGTAACAAGAGGGCTTGCAGTGTTGCCGGAAGACGGGGAAGTATTTCTGGTCGGAGACAACTTGGGAGGCGAGGGGTCGGTCAATTTGGAACCACTGGGAGCCCTTGCGCCATTGGTGGAGGTTGATTGTGGGGCGCATTTGGGGTTTGTAGCGGCCCCGACCCACTGGTCCTGGGAGGTCGTAGGACTCGACGAAGGTGGTCTTGGAGGCCATTAA

mRNA sequence

ATGGAGACGTTTCTGCTGCGGATCCATTTTCGGAACGCCGCCGTGCTCGTCACCTACGCCACCGCCTTCCTCCTCCGCTTTCGGCCGTCTCTCGCCGACGATAACGACGACTGTAAGATAGGCACGTGTCCTTTTCTTTTACCTCCTCCGCCAGACAATAGCGACGACATCAAAAAGAAGATTATCATTGGCACATGCACTGGTTTTGGAGCCTTGCTTTTAACCTTACTTGTTCTTGGCATCTGCTACCGATGGCGCCAGCTCCGTCGTAAAAGGTCTCACATGCTGCCATACGTGCAACGGAGCGTTCTCTCGAATCCCCCGTCCGTGGAAGAAGCTGAGAATGGAGGTACCTACTTGGGCGTTCACCTCTTCTCCTATAAAGAACTTGAAGAAGCCACGAACCATTTCGACTCCAACAAAGAGCTCGGCGATGGAGGCTTTGGCACAGTGCATTATGGCTTACTCAAAGACGGGCGTGCCGTTGCTGTCAAACGGTTGTTCGAAAGCAATTTCAAGAGAGTTGAGCAGTTCATGAATGAGGTCGGGATCCTCGCACGCTTACGCCACCACAACCTCGTTTCACTTTACGGTTGTACCTCGAGCAACAGCCGCGAGCTTCTGCTCGTGTATGAATACGTTCCGAACGGTACGGTGGCTGATCACCTCCATGGAAAACTAGCAAAGCCAAGCAAGCTTCCATGGTCTACAAGAATGAAGATTGCTATAGAGACTGCAAGTGCTTTGGTGTATCTCCATGCTTCTGAAATCATCCACCGTGATGTCAAAACCAACAACATCCTCCTCGACAACCACTACTCCGTTAAAGTAGCCGATTTCGGGTTATCCCGCCTCTTCCCACTCGACGTCACGCACGTTTCAACCGCTCCACAAGGTACTCCCGGCTACGTCGACCCGGAGTACCATCAGTGCTACCAACTCTCTGATAAAAGCGATGTCTTCAGCTTCGGCGTAGTACTTGTTGAACTCATGTCCTCAATGCCTGCTGTTGACATCACAAGACATAGACAAGAGATCAACCTGTTCAACATGGCCATCAACAAGATCCAAAGCCACGCATTACACGAACTCGTCGACCCATCGCTCGGTTTCGAATCAGACGACAAAATCAAAGACATGATAACGAGCGTCGCCGAGGTTGCATTTCAATGCCTGCAGAGCATGAAAGACGAGAGGCCAACAATGATGGAAGTTCTCGACAGTCTAAACGCCATACAGAATCGAAACGGTGATGCTGTGCAGCTTAGGAGCAAACAAGAGGAGCCTATTCAAGGAATTGGGCATGAATTTCCTGGCAAACAAATGGCATACATTGGTGTCAGCTCCATTGTACCGGCAGTGGCTGCCAGTCCTCAGTCTTTGGAGCAAGCCAACGTTGACATCCATTCTGTTGAACTTGGTGGGGTGGGCGCCGCCTTTGGACCAGTCGACCCAAGTCAAGGTCCGGTTGGAATTGGTGGTGGAGAAGTGGATTCCGACGAGGGTGGGGAGGTAGTGCTCGTCCATGTAACAAGAGGGCTTGCAGTGTTGCCGGAAGACGGGGAAGTATTTCTGGTCGGAGACAACTTGGGAGGCGAGGGGTCGGTCAATTTGGAACCACTGGGAGCCCTTGCGCCATTGGTGGAGGTTGATTGTGGGGCGCATTTGGGGTTTGTAGCGGCCCCGACCCACTGGTCCTGGGAGGTCGTAGGACTCGACGAAGGTGGTCTTGGAGGCCATTAA

Coding sequence (CDS)

ATGGAGACGTTTCTGCTGCGGATCCATTTTCGGAACGCCGCCGTGCTCGTCACCTACGCCACCGCCTTCCTCCTCCGCTTTCGGCCGTCTCTCGCCGACGATAACGACGACTGTAAGATAGGCACGTGTCCTTTTCTTTTACCTCCTCCGCCAGACAATAGCGACGACATCAAAAAGAAGATTATCATTGGCACATGCACTGGTTTTGGAGCCTTGCTTTTAACCTTACTTGTTCTTGGCATCTGCTACCGATGGCGCCAGCTCCGTCGTAAAAGGTCTCACATGCTGCCATACGTGCAACGGAGCGTTCTCTCGAATCCCCCGTCCGTGGAAGAAGCTGAGAATGGAGGTACCTACTTGGGCGTTCACCTCTTCTCCTATAAAGAACTTGAAGAAGCCACGAACCATTTCGACTCCAACAAAGAGCTCGGCGATGGAGGCTTTGGCACAGTGCATTATGGCTTACTCAAAGACGGGCGTGCCGTTGCTGTCAAACGGTTGTTCGAAAGCAATTTCAAGAGAGTTGAGCAGTTCATGAATGAGGTCGGGATCCTCGCACGCTTACGCCACCACAACCTCGTTTCACTTTACGGTTGTACCTCGAGCAACAGCCGCGAGCTTCTGCTCGTGTATGAATACGTTCCGAACGGTACGGTGGCTGATCACCTCCATGGAAAACTAGCAAAGCCAAGCAAGCTTCCATGGTCTACAAGAATGAAGATTGCTATAGAGACTGCAAGTGCTTTGGTGTATCTCCATGCTTCTGAAATCATCCACCGTGATGTCAAAACCAACAACATCCTCCTCGACAACCACTACTCCGTTAAAGTAGCCGATTTCGGGTTATCCCGCCTCTTCCCACTCGACGTCACGCACGTTTCAACCGCTCCACAAGGTACTCCCGGCTACGTCGACCCGGAGTACCATCAGTGCTACCAACTCTCTGATAAAAGCGATGTCTTCAGCTTCGGCGTAGTACTTGTTGAACTCATGTCCTCAATGCCTGCTGTTGACATCACAAGACATAGACAAGAGATCAACCTGTTCAACATGGCCATCAACAAGATCCAAAGCCACGCATTACACGAACTCGTCGACCCATCGCTCGGTTTCGAATCAGACGACAAAATCAAAGACATGATAACGAGCGTCGCCGAGGTTGCATTTCAATGCCTGCAGAGCATGAAAGACGAGAGGCCAACAATGATGGAAGTTCTCGACAGTCTAAACGCCATACAGAATCGAAACGGTGATGCTGTGCAGCTTAGGAGCAAACAAGAGGAGCCTATTCAAGGAATTGGGCATGAATTTCCTGGCAAACAAATGGCATACATTGGTGTCAGCTCCATTGTACCGGCAGTGGCTGCCAGTCCTCAGTCTTTGGAGCAAGCCAACGTTGACATCCATTCTGTTGAACTTGGTGGGGTGGGCGCCGCCTTTGGACCAGTCGACCCAAGTCAAGGTCCGGTTGGAATTGGTGGTGGAGAAGTGGATTCCGACGAGGGTGGGGAGGTAGTGCTCGTCCATGTAACAAGAGGGCTTGCAGTGTTGCCGGAAGACGGGGAAGTATTTCTGGTCGGAGACAACTTGGGAGGCGAGGGGTCGGTCAATTTGGAACCACTGGGAGCCCTTGCGCCATTGGTGGAGGTTGATTGTGGGGCGCATTTGGGGTTTGTAGCGGCCCCGACCCACTGGTCCTGGGAGGTCGTAGGACTCGACGAAGGTGGTCTTGGAGGCCATTAA

Protein sequence

METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKKIIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAVQLRSKQEEPIQGIGHEFPGKQMAYIGVSSIVPAVAASPQSLEQANVDIHSVELGGVGAAFGPVDPSQGPVGIGGGEVDSDEGGEVVLVHVTRGLAVLPEDGEVFLVGDNLGGEGSVNLEPLGALAPLVEVDCGAHLGFVAAPTHWSWEVVGLDEGGLGGH
Homology
BLAST of Carg15159-RA vs. NCBI nr
Match: KAG7023305.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60
           METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK
Sbjct: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60

Query: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120
           IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL
Sbjct: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120

Query: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180
           GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN
Sbjct: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180

Query: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK 240
           EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK
Sbjct: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK 240

Query: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300
           IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT
Sbjct: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300

Query: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360
           PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA
Sbjct: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360

Query: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420
           LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV
Sbjct: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420

Query: 421 QLRSKQEEPIQGIGHEFPGKQMAYIGVSSIVPAVAASPQSLEQANVDIHSVELGGVGAAF 480
           QLRSKQEEPIQGIGHEFPGKQMAYIGVSSIVPAVAASPQSLEQANVDIHSVELGGVGAAF
Sbjct: 421 QLRSKQEEPIQGIGHEFPGKQMAYIGVSSIVPAVAASPQSLEQANVDIHSVELGGVGAAF 480

Query: 481 GPVDPSQGPVGIGGGEVDSDEGGEVVLVHVTRGLAVLPEDGEVFLVGDNLGGEGSVNLEP 540
           GPVDPSQGPVGIGGGEVDSDEGGEVVLVHVTRGLAVLPEDGEVFLVGDNLGGEGSVNLEP
Sbjct: 481 GPVDPSQGPVGIGGGEVDSDEGGEVVLVHVTRGLAVLPEDGEVFLVGDNLGGEGSVNLEP 540

Query: 541 LGALAPLVEVDCGAHLGFVAAPTHWSWEVVGLDEGGLGGH 581
           LGALAPLVEVDCGAHLGFVAAPTHWSWEVVGLDEGGLGGH
Sbjct: 541 LGALAPLVEVDCGAHLGFVAAPTHWSWEVVGLDEGGLGGH 580

BLAST of Carg15159-RA vs. NCBI nr
Match: KAG6589614.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 849.4 bits (2193), Expect = 1.9e-242
Identity = 421/427 (98.59%), Postives = 425/427 (99.53%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60
           METFLLRIHFRNAAVLVT+ATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK
Sbjct: 1   METFLLRIHFRNAAVLVTFATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60

Query: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120
           IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL
Sbjct: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120

Query: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180
           GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN
Sbjct: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180

Query: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK 240
           EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK
Sbjct: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK 240

Query: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300
           IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT
Sbjct: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300

Query: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360
           PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA
Sbjct: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360

Query: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420
           LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV
Sbjct: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420

Query: 421 QLRSKQE 428
           Q+++  E
Sbjct: 421 QVKNGYE 427

BLAST of Carg15159-RA vs. NCBI nr
Match: XP_022921940.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cucurbita moschata])

HSP 1 Score: 843.6 bits (2178), Expect = 1.0e-240
Identity = 419/425 (98.59%), Postives = 423/425 (99.53%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60
           METFLLRIHFRNAAVLVT+ATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK
Sbjct: 1   METFLLRIHFRNAAVLVTFATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60

Query: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120
           IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL
Sbjct: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120

Query: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180
           GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN
Sbjct: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180

Query: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK 240
           EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAK SKLPWSTRMK
Sbjct: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKLSKLPWSTRMK 240

Query: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300
           IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT
Sbjct: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300

Query: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360
           PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA
Sbjct: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360

Query: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420
           LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV
Sbjct: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420

Query: 421 QLRSK 426
           Q +++
Sbjct: 421 QEQTR 425

BLAST of Carg15159-RA vs. NCBI nr
Match: XP_022988480.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 820.8 bits (2119), Expect = 7.2e-234
Identity = 409/428 (95.56%), Postives = 417/428 (97.43%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDC-KIGTCPFLLPPPPDNSDDIKK 60
           M+TFLLRIHFRNAAVLVT+ATAFLLRF PSLADD DDC K GTC FLLPPPPDNSDDIKK
Sbjct: 1   MKTFLLRIHFRNAAVLVTFATAFLLRFPPSLADDEDDCFKTGTCSFLLPPPPDNSDDIKK 60

Query: 61  KIIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTY 120
           KIIIGTCTGFGALLLTLLVLG+CYRWRQLRRKRSHMLPYVQ+SVLSNPPSVEEAENGGTY
Sbjct: 61  KIIIGTCTGFGALLLTLLVLGVCYRWRQLRRKRSHMLPYVQQSVLSNPPSVEEAENGGTY 120

Query: 121 LGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFM 180
           LGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFM
Sbjct: 121 LGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFM 180

Query: 181 NEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRM 240
           NEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKP KLPWSTR+
Sbjct: 181 NEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPGKLPWSTRL 240

Query: 241 KIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQG 300
           KIAIETA ALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQG
Sbjct: 241 KIAIETAGALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQG 300

Query: 301 TPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSH 360
           TPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSH
Sbjct: 301 TPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSH 360

Query: 361 ALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDA 420
           ALHELVDPSLGFESD KIKDMITSVAEVAFQCLQSMKDERPTM+EVLDSLNAIQNRN DA
Sbjct: 361 ALHELVDPSLGFESDYKIKDMITSVAEVAFQCLQSMKDERPTMIEVLDSLNAIQNRNNDA 420

Query: 421 VQLRSKQE 428
           VQL++  E
Sbjct: 421 VQLKNGYE 428

BLAST of Carg15159-RA vs. NCBI nr
Match: XP_022988479.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 816.2 bits (2107), Expect = 1.8e-232
Identity = 409/429 (95.34%), Postives = 417/429 (97.20%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDC-KIGTCPFLLPPPP-DNSDDIK 60
           M+TFLLRIHFRNAAVLVT+ATAFLLRF PSLADD DDC K GTC FLLPPPP DNSDDIK
Sbjct: 1   MKTFLLRIHFRNAAVLVTFATAFLLRFPPSLADDEDDCFKTGTCSFLLPPPPTDNSDDIK 60

Query: 61  KKIIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGT 120
           KKIIIGTCTGFGALLLTLLVLG+CYRWRQLRRKRSHMLPYVQ+SVLSNPPSVEEAENGGT
Sbjct: 61  KKIIIGTCTGFGALLLTLLVLGVCYRWRQLRRKRSHMLPYVQQSVLSNPPSVEEAENGGT 120

Query: 121 YLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQF 180
           YLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQF
Sbjct: 121 YLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQF 180

Query: 181 MNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTR 240
           MNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKP KLPWSTR
Sbjct: 181 MNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPGKLPWSTR 240

Query: 241 MKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQ 300
           +KIAIETA ALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQ
Sbjct: 241 LKIAIETAGALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQ 300

Query: 301 GTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQS 360
           GTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQS
Sbjct: 301 GTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQS 360

Query: 361 HALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGD 420
           HALHELVDPSLGFESD KIKDMITSVAEVAFQCLQSMKDERPTM+EVLDSLNAIQNRN D
Sbjct: 361 HALHELVDPSLGFESDYKIKDMITSVAEVAFQCLQSMKDERPTMIEVLDSLNAIQNRNND 420

Query: 421 AVQLRSKQE 428
           AVQL++  E
Sbjct: 421 AVQLKNGYE 429

BLAST of Carg15159-RA vs. ExPASy Swiss-Prot
Match: P0C5E2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.2 PE=2 SV=3)

HSP 1 Score: 459.1 bits (1180), Expect = 7.2e-128
Identity = 244/419 (58.23%), Postives = 309/419 (73.75%), Query Frame = 0

Query: 52  DNSDDIKKKIIIGTCTGFGALLLTLLVLGI---CYRWRQLR--RKRSHMLPYVQRSVLSN 111
           +  +D ++++I+    G  A ++ L+   I    Y  R+ +  R  S +LP   R++ S+
Sbjct: 252 NGKNDKRRRVIVKVLIGASAAVVGLIAASIFWYVYHRRKTKSYRNSSALLP---RNISSD 311

Query: 112 PPS----VEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAV 171
           P +    +E+AE     +GVH+FSY+ELEEATN+FD +KELGDGGFGTV+YG LKDGR+V
Sbjct: 312 PSAKSFDIEKAEE--LLVGVHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSV 371

Query: 172 AVKRLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADH 231
           AVKRL+++NFKR EQF NEV IL  LRH NLV+L+GC+S  SR+LLLVYEYV NGT+ADH
Sbjct: 372 AVKRLYDNNFKRAEQFRNEVEILTGLRHPNLVALFGCSSKQSRDLLLVYEYVANGTLADH 431

Query: 232 LHGKLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGL 291
           LHG  A PS LPWS R+KIA+ETASAL YLHAS+IIHRDVK+NNILLD +++VKVADFGL
Sbjct: 432 LHGPQANPSSLPWSIRLKIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGL 491

Query: 292 SRLFPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRH 351
           SRLFP+D THVSTAPQGTPGYVDP+YH CYQLS+KSDV+SF VVL+EL+SS+PAVDITR 
Sbjct: 492 SRLFPMDKTHVSTAPQGTPGYVDPDYHLCYQLSNKSDVYSFAVVLMELISSLPAVDITRP 551

Query: 352 RQEINLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTM 411
           RQEINL NMA+ KIQ+H L ++VDPSLGF++D +++  + +VAE+AFQCLQS KD RP M
Sbjct: 552 RQEINLSNMAVVKIQNHELRDMVDPSLGFDTDTRVRQTVIAVAELAFQCLQSDKDLRPCM 611

Query: 412 MEVLDSLNAIQNRNGDAVQLRSKQEEPIQGIGHEFPGKQMAYIGVSSIVPAVAASPQSL 462
             V D+L  IQN NG                     G +M  + V+   P VA SP S+
Sbjct: 612 SHVQDTLTRIQN-NGF--------------------GSEMDVVDVNKSGPLVAQSPDSV 644

BLAST of Carg15159-RA vs. ExPASy Swiss-Prot
Match: Q8VYG0 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.3 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.3 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 3.0e-118
Identity = 227/377 (60.21%), Postives = 276/377 (73.21%), Query Frame = 0

Query: 58  KKKIIIGTCTGF-GALLLTLLVLGICYRWRQ------LRRKRSHMLPYVQRSVLSNP--- 117
           K KI IG  +GF GA L+   +L I  R R+        +  S   PY     +SN    
Sbjct: 260 KGKIGIGFASGFLGATLIGGCLLCIFIRRRKKLATQYTNKGLSTTTPYSSNYTMSNTPTS 319

Query: 118 -----------PSVEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLL 177
                      PS+    NG  Y G+ +FSY+ELEEAT +F  +KELGDGGFGTV+YG L
Sbjct: 320 TTISGSNHSLVPSISNLGNGSVYSGIQVFSYEELEEATENF--SKELGDGGFGTVYYGTL 379

Query: 178 KDGRAVAVKRLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPN 237
           KDGRAVAVKRLFE + KRVEQF NE+ IL  L+H NLV LYGCT+ +SRELLLVYEY+ N
Sbjct: 380 KDGRAVAVKRLFERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSRELLLVYEYISN 439

Query: 238 GTVADHLHGKLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVK 297
           GT+A+HLHG  A+   + W  R++IAIETASAL YLHAS IIHRDVKT NILLD++Y VK
Sbjct: 440 GTLAEHLHGNQAQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTTNILLDSNYQVK 499

Query: 298 VADFGLSRLFPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPA 357
           VADFGLSRLFP+D TH+STAPQGTPGYVDPEY+QCY+L++KSDV+SFGVVL EL+SS  A
Sbjct: 500 VADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGVVLSELISSKEA 559

Query: 358 VDITRHRQEINLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMK 414
           VDITRHR +INL NMAI+KIQ+ A+HEL D SLGF  D  +K M++SVAE+AF+CLQ  +
Sbjct: 560 VDITRHRHDINLANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVAELAFRCLQQER 619

BLAST of Carg15159-RA vs. ExPASy Swiss-Prot
Match: F4HQ17 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.4 PE=2 SV=2)

HSP 1 Score: 426.0 bits (1094), Expect = 6.7e-118
Identity = 224/395 (56.71%), Postives = 280/395 (70.89%), Query Frame = 0

Query: 50  PPDNSDDIKKKIIIGTCTGFGALLLTLLVLGICYRWR------QLRRKRSHMLPYVQRSV 109
           P D S  I  K  I   +  G  +L L  L +C R R      Q   K   +  Y  R  
Sbjct: 229 PTDTSLSIGAKAGIAVASVSGLAILLLAGLFLCIRRRRKTQDAQYTSKSLPITSYSSRDT 288

Query: 110 LSNP--------------PSVEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFG 169
             NP              PS+    N   Y GV +FSY+ELEEAT +F  ++ELGDGGFG
Sbjct: 289 SRNPTSTTISSSSNHSLLPSISNLANRSDYCGVQVFSYEELEEATENF--SRELGDGGFG 348

Query: 170 TVHYGLLKDGRAVAVKRLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLL 229
           TV+YG+LKDGRAVAVKRL+E + KRVEQF NE+ IL  L+H NLV LYGCTS +SRELLL
Sbjct: 349 TVYYGVLKDGRAVAVKRLYERSLKRVEQFKNEIEILKSLKHPNLVILYGCTSRHSRELLL 408

Query: 230 VYEYVPNGTVADHLHGKLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILL 289
           VYEY+ NGT+A+HLHG  A+   L WSTR+ IAIETASAL +LH   IIHRD+KT NILL
Sbjct: 409 VYEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASALSFLHIKGIIHRDIKTTNILL 468

Query: 290 DNHYSVKVADFGLSRLFPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVE 349
           D++Y VKVADFGLSRLFP+D TH+STAPQGTPGYVDPEY+QCYQL++KSDV+SFGVVL E
Sbjct: 469 DDNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYQLNEKSDVYSFGVVLTE 528

Query: 350 LMSSMPAVDITRHRQEINLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAF 409
           L+SS  AVDITRHR +INL NMA++KIQ++ALHELVD SLG+++D +++  + +VAE+AF
Sbjct: 529 LISSKEAVDITRHRHDINLANMAVSKIQNNALHELVDSSLGYDNDPEVRRKMMAVAELAF 588

Query: 410 QCLQSMKDERPTMMEVLDSLNAIQNRNGDAVQLRS 425
           +CLQ  +D RP M E+++ L  I++     V ++S
Sbjct: 589 RCLQQERDVRPAMDEIVEILRGIKDDEKKRVLVKS 621

BLAST of Carg15159-RA vs. ExPASy Swiss-Prot
Match: Q9C6K9 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.1 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-1.1 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 9.1e-115
Identity = 213/353 (60.34%), Postives = 262/353 (74.22%), Query Frame = 0

Query: 63  IGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYLGV 122
           +G   G   +L+ +LV       R  RRK          S LS   S  + E    +  +
Sbjct: 225 LGLGIGGSVILIIILVALFAVIHRNYRRK--------DGSELSRDNSKSDVEFSQVFFKI 284

Query: 123 HLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMNEV 182
            +FSYKEL+ AT++F  ++ LGDGGFGTV+YG ++DGR VAVKRL+E N++R+EQFMNE+
Sbjct: 285 PIFSYKELQAATDNFSKDRLLGDGGFGTVYYGKVRDGREVAVKRLYEHNYRRLEQFMNEI 344

Query: 183 GILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGK-LAKPSKLPWSTRMKI 242
            IL RL H NLVSLYGCTS  SRELLLVYE++PNGTVADHL+G+       L WS R+ I
Sbjct: 345 EILTRLHHKNLVSLYGCTSRRSRELLLVYEFIPNGTVADHLYGENTPHQGFLTWSMRLSI 404

Query: 243 AIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGTP 302
           AIETASAL YLHAS+IIHRDVKT NILLD ++ VKVADFGLSRL P DVTHVSTAPQGTP
Sbjct: 405 AIETASALAYLHASDIIHRDVKTTNILLDRNFGVKVADFGLSRLLPSDVTHVSTAPQGTP 464

Query: 303 GYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHAL 362
           GYVDPEYH+CY L+DKSDV+SFGVVLVEL+SS PAVDI+R + EINL ++AINKIQ+HA 
Sbjct: 465 GYVDPEYHRCYHLTDKSDVYSFGVVLVELISSKPAVDISRCKSEINLSSLAINKIQNHAT 524

Query: 363 HELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQN 415
           HEL+D +LG+ +++ ++ M T VAE+AFQCLQ     RPTM +V+  L  IQN
Sbjct: 525 HELIDQNLGYATNEGVRKMTTMVAELAFQCLQQDNTMRPTMEQVVHELKGIQN 569

BLAST of Carg15159-RA vs. ExPASy Swiss-Prot
Match: Q8RY67 (Wall-associated receptor kinase-like 14 OS=Arabidopsis thaliana OX=3702 GN=WAKL14 PE=2 SV=2)

HSP 1 Score: 285.4 bits (729), Expect = 1.4e-75
Identity = 161/355 (45.35%), Postives = 224/355 (63.10%), Query Frame = 0

Query: 62  IIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYLG 121
           I+G   G   LL  L     C      +R+RS  L    RS LS    + EA    +   
Sbjct: 286 IVGGTVGGAFLLAALAFFFFC------KRRRSTPL----RSHLSAKRLLSEAAGNSS--- 345

Query: 122 VHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMNE 181
           V  F YKE+E+AT+ F   ++LG G +GTV+ G L++   VA+KRL   + + ++Q MNE
Sbjct: 346 VAFFPYKEIEKATDGFSEKQKLGIGAYGTVYRGKLQNDEWVAIKRLRHRDSESLDQVMNE 405

Query: 182 VGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMKI 241
           + +L+ + H NLV L GC      + +LVYEY+PNGT+++HL  +  + S LPW+ R+ +
Sbjct: 406 IKLLSSVSHPNLVRLLGCCIEQG-DPVLVYEYMPNGTLSEHL--QRDRGSGLPWTLRLTV 465

Query: 242 AIETASALVYLHAS---EIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQ 301
           A +TA A+ YLH+S    I HRD+K+ NILLD  ++ KVADFGLSRL   + +H+STAPQ
Sbjct: 466 ATQTAKAIAYLHSSMNPPIYHRDIKSTNILLDYDFNSKVADFGLSRLGMTESSHISTAPQ 525

Query: 302 GTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQS 361
           GTPGY+DP+YHQC+ LSDKSDV+SFGVVL E+++ +  VD TR   EINL  +A++KI S
Sbjct: 526 GTPGYLDPQYHQCFHLSDKSDVYSFGVVLAEIITGLKVVDFTRPHTEINLAALAVDKIGS 585

Query: 362 HALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQ 414
             + E++DP L  + D      I +VAE+AF+CL    D RPTM EV D L  I+
Sbjct: 586 GCIDEIIDPILDLDLDAWTLSSIHTVAELAFRCLAFHSDMRPTMTEVADELEQIR 624

BLAST of Carg15159-RA vs. ExPASy TrEMBL
Match: A0A6J1E770 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Cucurbita moschata OX=3662 GN=LOC111430046 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 5.0e-241
Identity = 419/425 (98.59%), Postives = 423/425 (99.53%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60
           METFLLRIHFRNAAVLVT+ATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK
Sbjct: 1   METFLLRIHFRNAAVLVTFATAFLLRFRPSLADDNDDCKIGTCPFLLPPPPDNSDDIKKK 60

Query: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120
           IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL
Sbjct: 61  IIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYL 120

Query: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180
           GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN
Sbjct: 121 GVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMN 180

Query: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMK 240
           EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAK SKLPWSTRMK
Sbjct: 181 EVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKLSKLPWSTRMK 240

Query: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300
           IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT
Sbjct: 241 IAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGT 300

Query: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360
           PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA
Sbjct: 301 PGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHA 360

Query: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420
           LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV
Sbjct: 361 LHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAV 420

Query: 421 QLRSK 426
           Q +++
Sbjct: 421 QEQTR 425

BLAST of Carg15159-RA vs. ExPASy TrEMBL
Match: A0A6J1JJN6 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485709 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 3.5e-234
Identity = 409/428 (95.56%), Postives = 417/428 (97.43%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDC-KIGTCPFLLPPPPDNSDDIKK 60
           M+TFLLRIHFRNAAVLVT+ATAFLLRF PSLADD DDC K GTC FLLPPPPDNSDDIKK
Sbjct: 1   MKTFLLRIHFRNAAVLVTFATAFLLRFPPSLADDEDDCFKTGTCSFLLPPPPDNSDDIKK 60

Query: 61  KIIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTY 120
           KIIIGTCTGFGALLLTLLVLG+CYRWRQLRRKRSHMLPYVQ+SVLSNPPSVEEAENGGTY
Sbjct: 61  KIIIGTCTGFGALLLTLLVLGVCYRWRQLRRKRSHMLPYVQQSVLSNPPSVEEAENGGTY 120

Query: 121 LGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFM 180
           LGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFM
Sbjct: 121 LGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFM 180

Query: 181 NEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRM 240
           NEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKP KLPWSTR+
Sbjct: 181 NEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPGKLPWSTRL 240

Query: 241 KIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQG 300
           KIAIETA ALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQG
Sbjct: 241 KIAIETAGALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQG 300

Query: 301 TPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSH 360
           TPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSH
Sbjct: 301 TPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSH 360

Query: 361 ALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDA 420
           ALHELVDPSLGFESD KIKDMITSVAEVAFQCLQSMKDERPTM+EVLDSLNAIQNRN DA
Sbjct: 361 ALHELVDPSLGFESDYKIKDMITSVAEVAFQCLQSMKDERPTMIEVLDSLNAIQNRNNDA 420

Query: 421 VQLRSKQE 428
           VQL++  E
Sbjct: 421 VQLKNGYE 428

BLAST of Carg15159-RA vs. ExPASy TrEMBL
Match: A0A6J1JLM7 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485709 PE=3 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 8.6e-233
Identity = 409/429 (95.34%), Postives = 417/429 (97.20%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLADDNDDC-KIGTCPFLLPPPP-DNSDDIK 60
           M+TFLLRIHFRNAAVLVT+ATAFLLRF PSLADD DDC K GTC FLLPPPP DNSDDIK
Sbjct: 1   MKTFLLRIHFRNAAVLVTFATAFLLRFPPSLADDEDDCFKTGTCSFLLPPPPTDNSDDIK 60

Query: 61  KKIIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGT 120
           KKIIIGTCTGFGALLLTLLVLG+CYRWRQLRRKRSHMLPYVQ+SVLSNPPSVEEAENGGT
Sbjct: 61  KKIIIGTCTGFGALLLTLLVLGVCYRWRQLRRKRSHMLPYVQQSVLSNPPSVEEAENGGT 120

Query: 121 YLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQF 180
           YLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQF
Sbjct: 121 YLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQF 180

Query: 181 MNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTR 240
           MNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKP KLPWSTR
Sbjct: 181 MNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPGKLPWSTR 240

Query: 241 MKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQ 300
           +KIAIETA ALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQ
Sbjct: 241 LKIAIETAGALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQ 300

Query: 301 GTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQS 360
           GTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQS
Sbjct: 301 GTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQS 360

Query: 361 HALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGD 420
           HALHELVDPSLGFESD KIKDMITSVAEVAFQCLQSMKDERPTM+EVLDSLNAIQNRN D
Sbjct: 361 HALHELVDPSLGFESDYKIKDMITSVAEVAFQCLQSMKDERPTMIEVLDSLNAIQNRNND 420

Query: 421 AVQLRSKQE 428
           AVQL++  E
Sbjct: 421 AVQLKNGYE 429

BLAST of Carg15159-RA vs. ExPASy TrEMBL
Match: A0A1S4DZK4 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494139 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 1.0e-185
Identity = 344/427 (80.56%), Postives = 363/427 (85.01%), Query Frame = 0

Query: 1   METFLLRIHFRNAAVLVTYATAFLLRFRPSLA-DDNDDCKIGTCPFLLPPPPDNSDDIKK 60
           M  FL   H+  A  L   A   LL F+PSLA DD  DC  G   +   PPP  +   +K
Sbjct: 1   MNKFL--FHYPTALFLFATAFLLLLLFQPSLAEDDLPDCPTGLRCYFPSPPPSGNGRTRK 60

Query: 61  KIIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNP-----PS-VEEA 120
           KIIIG C+  GALLL  LVLGICY +RQ RR+RSH LPYVQRS+ SNP     PS VEE 
Sbjct: 61  KIIIGVCSSVGALLLIFLVLGICYLYRQRRRRRSHALPYVQRSISSNPSNPTNPSPVEEV 120

Query: 121 ENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFK 180
           ENGGTYLGV LFSYKELEEATNHFDSNKELGDGGFGTV+YGLLKDGRAVAVKRLFE NFK
Sbjct: 121 ENGGTYLGVQLFSYKELEEATNHFDSNKELGDGGFGTVYYGLLKDGRAVAVKRLFEGNFK 180

Query: 181 RVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGKLAKPSKL 240
           RVEQFMNEV ILARLRH NLVSLYGCTS +SRELLLVYEYVPNGTVADHLHGKLAK  KL
Sbjct: 181 RVEQFMNEVEILARLRHRNLVSLYGCTSRSSRELLLVYEYVPNGTVADHLHGKLAKSGKL 240

Query: 241 PWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHV 300
           PWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDN Y VKVADFGLSRLFPLDVTHV
Sbjct: 241 PWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNTYCVKVADFGLSRLFPLDVTHV 300

Query: 301 STAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAI 360
           STAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVEL+SSMPAVDITRHRQEINLFNMAI
Sbjct: 301 STAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELISSMPAVDITRHRQEINLFNMAI 360

Query: 361 NKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQ 420
           NKIQ+ ALHE VDPSLGFESD KI++MITSVAE+AF+CLQSMKDERPTMMEVLD+LN IQ
Sbjct: 361 NKIQNCALHEFVDPSLGFESDYKIQEMITSVAELAFRCLQSMKDERPTMMEVLDTLNIIQ 420

BLAST of Carg15159-RA vs. ExPASy TrEMBL
Match: A0A6J1C0J9 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=Momordica charantia OX=3673 GN=LOC111006965 PE=4 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 2.4e-179
Identity = 321/373 (86.06%), Postives = 342/373 (91.69%), Query Frame = 0

Query: 52  DNSDDIKKKIIIGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLS------ 111
           DN  DI+ KIIIGTC GFG LLLTLLVLGI YR RQLR K S  LPYVQRS+ S      
Sbjct: 262 DNKHDIRNKIIIGTCVGFGTLLLTLLVLGIWYR-RQLRHKTSRTLPYVQRSIPSDPLNPP 321

Query: 112 NPPSVEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVK 171
           NPPSVEE E GGTYLGVHLFSYKELEEAT+HFDSNKELGDGGFGTV++GLLKDGRAVAVK
Sbjct: 322 NPPSVEELEKGGTYLGVHLFSYKELEEATHHFDSNKELGDGGFGTVYHGLLKDGRAVAVK 381

Query: 172 RLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHG 231
           RLFESNFKRVEQFMNEV ILARLRH NLVSLYGCTS +SRELLLVYEY+PNGTVADHLHG
Sbjct: 382 RLFESNFKRVEQFMNEVDILARLRHRNLVSLYGCTSRHSRELLLVYEYIPNGTVADHLHG 441

Query: 232 KLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRL 291
           KLAKP KLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDN++ VKVADFGLSRL
Sbjct: 442 KLAKPGKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNNFCVKVADFGLSRL 501

Query: 292 FPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQE 351
           FPLDV+H+ST PQGTPGYVDPEYHQCYQLSD+SDVFSFGVVLVELMSSMPAVDITRHRQE
Sbjct: 502 FPLDVSHISTTPQGTPGYVDPEYHQCYQLSDRSDVFSFGVVLVELMSSMPAVDITRHRQE 561

Query: 352 INLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEV 411
           INLFNMAINKIQSHALHELVDP+LGFESD KI++MITSVAE+AF+CLQ MKDERP+MMEV
Sbjct: 562 INLFNMAINKIQSHALHELVDPTLGFESDYKIREMITSVAELAFRCLQGMKDERPSMMEV 621

Query: 412 LDSLNAIQNRNGD 419
           L++LN IQ +N D
Sbjct: 622 LETLNTIQKQNTD 633

BLAST of Carg15159-RA vs. TAIR 10
Match: AT1G18390.1 (Protein kinase superfamily protein )

HSP 1 Score: 458.8 bits (1179), Expect = 6.7e-129
Identity = 244/416 (58.65%), Postives = 308/416 (74.04%), Query Frame = 0

Query: 55  DDIKKKIIIGTCTGFGALLLTLLVLGI---CYRWRQLR--RKRSHMLPYVQRSVLSNPPS 114
           +D ++++I+    G  A ++ L+   I    Y  R+ +  R  S +LP   R++ S+P +
Sbjct: 252 NDKRRRVIVKVLIGASAAVVGLIAASIFWYVYHRRKTKSYRNSSALLP---RNISSDPSA 311

Query: 115 ----VEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVK 174
               +E+AE     +GVH+FSY+ELEEATN+FD +KELGDGGFGTV+YG LKDGR+VAVK
Sbjct: 312 KSFDIEKAEE--LLVGVHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDGRSVAVK 371

Query: 175 RLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHG 234
           RL+++NFKR EQF NEV IL  LRH NLV+L+GC+S  SR+LLLVYEYV NGT+ADHLHG
Sbjct: 372 RLYDNNFKRAEQFRNEVEILTGLRHPNLVALFGCSSKQSRDLLLVYEYVANGTLADHLHG 431

Query: 235 KLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRL 294
             A PS LPWS R+KIA+ETASAL YLHAS+IIHRDVK+NNILLD +++VKVADFGLSRL
Sbjct: 432 PQANPSSLPWSIRLKIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVKVADFGLSRL 491

Query: 295 FPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQE 354
           FP+D THVSTAPQGTPGYVDP+YH CYQLS+KSDV+SF VVL+EL+SS+PAVDITR RQE
Sbjct: 492 FPMDKTHVSTAPQGTPGYVDPDYHLCYQLSNKSDVYSFAVVLMELISSLPAVDITRPRQE 551

Query: 355 INLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEV 414
           INL NMA+ KIQ+H L ++VDPSLGF++D +++  + +VAE+AFQCLQS KD RP M  V
Sbjct: 552 INLSNMAVVKIQNHELRDMVDPSLGFDTDTRVRQTVIAVAELAFQCLQSDKDLRPCMSHV 611

Query: 415 LDSLNAIQNRNGDAVQLRSKQEEPIQGIGHEFPGKQMAYIGVSSIVPAVAASPQSL 462
            D+L  IQN NG                     G +M  + V+   P VA SP S+
Sbjct: 612 QDTLTRIQN-NGF--------------------GSEMDVVDVNKSGPLVAQSPDSV 641

BLAST of Carg15159-RA vs. TAIR 10
Match: AT1G18390.2 (Protein kinase superfamily protein )

HSP 1 Score: 454.9 bits (1169), Expect = 9.6e-128
Identity = 244/422 (57.82%), Postives = 311/422 (73.70%), Query Frame = 0

Query: 52  DNSDDIKKKIII---GTCTGFGALLLTLLVLGI---CYRWRQLR--RKRSHMLPYVQRSV 111
           +  +D ++++I+    + +G  A ++ L+   I    Y  R+ +  R  S +LP   R++
Sbjct: 252 NGKNDKRRRVIVKITKSISGASAAVVGLIAASIFWYVYHRRKTKSYRNSSALLP---RNI 311

Query: 112 LSNPPS----VEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDG 171
            S+P +    +E+AE     +GVH+FSY+ELEEATN+FD +KELGDGGFGTV+YG LKDG
Sbjct: 312 SSDPSAKSFDIEKAEE--LLVGVHIFSYEELEEATNNFDPSKELGDGGFGTVYYGKLKDG 371

Query: 172 RAVAVKRLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTV 231
           R+VAVKRL+++NFKR EQF NEV IL  LRH NLV+L+GC+S  SR+LLLVYEYV NGT+
Sbjct: 372 RSVAVKRLYDNNFKRAEQFRNEVEILTGLRHPNLVALFGCSSKQSRDLLLVYEYVANGTL 431

Query: 232 ADHLHGKLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVAD 291
           ADHLHG  A PS LPWS R+KIA+ETASAL YLHAS+IIHRDVK+NNILLD +++VKVAD
Sbjct: 432 ADHLHGPQANPSSLPWSIRLKIAVETASALKYLHASKIIHRDVKSNNILLDQNFNVKVAD 491

Query: 292 FGLSRLFPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDI 351
           FGLSRLFP+D THVSTAPQGTPGYVDP+YH CYQLS+KSDV+SF VVL+EL+SS+PAVDI
Sbjct: 492 FGLSRLFPMDKTHVSTAPQGTPGYVDPDYHLCYQLSNKSDVYSFAVVLMELISSLPAVDI 551

Query: 352 TRHRQEINLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDER 411
           TR RQEINL NMA+ KIQ+H L ++VDPSLGF++D +++  + +VAE+AFQCLQS KD R
Sbjct: 552 TRPRQEINLSNMAVVKIQNHELRDMVDPSLGFDTDTRVRQTVIAVAELAFQCLQSDKDLR 611

Query: 412 PTMMEVLDSLNAIQNRNGDAVQLRSKQEEPIQGIGHEFPGKQMAYIGVSSIVPAVAASPQ 462
           P M  V D+L  IQN NG                     G +M  + V+   P VA SP 
Sbjct: 612 PCMSHVQDTLTRIQN-NGF--------------------GSEMDVVDVNKSGPLVAQSPD 647

BLAST of Carg15159-RA vs. TAIR 10
Match: AT5G38210.1 (Protein kinase family protein )

HSP 1 Score: 427.2 bits (1097), Expect = 2.2e-119
Identity = 227/377 (60.21%), Postives = 276/377 (73.21%), Query Frame = 0

Query: 58  KKKIIIGTCTGF-GALLLTLLVLGICYRWRQ------LRRKRSHMLPYVQRSVLSNP--- 117
           K KI IG  +GF GA L+   +L I  R R+        +  S   PY     +SN    
Sbjct: 260 KGKIGIGFASGFLGATLIGGCLLCIFIRRRKKLATQYTNKGLSTTTPYSSNYTMSNTPTS 319

Query: 118 -----------PSVEEAENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVHYGLL 177
                      PS+    NG  Y G+ +FSY+ELEEAT +F  +KELGDGGFGTV+YG L
Sbjct: 320 TTISGSNHSLVPSISNLGNGSVYSGIQVFSYEELEEATENF--SKELGDGGFGTVYYGTL 379

Query: 178 KDGRAVAVKRLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSNSRELLLVYEYVPN 237
           KDGRAVAVKRLFE + KRVEQF NE+ IL  L+H NLV LYGCT+ +SRELLLVYEY+ N
Sbjct: 380 KDGRAVAVKRLFERSLKRVEQFKNEIDILKSLKHPNLVILYGCTTRHSRELLLVYEYISN 439

Query: 238 GTVADHLHGKLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNHYSVK 297
           GT+A+HLHG  A+   + W  R++IAIETASAL YLHAS IIHRDVKT NILLD++Y VK
Sbjct: 440 GTLAEHLHGNQAQSRPICWPARLQIAIETASALSYLHASGIIHRDVKTTNILLDSNYQVK 499

Query: 298 VADFGLSRLFPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPA 357
           VADFGLSRLFP+D TH+STAPQGTPGYVDPEY+QCY+L++KSDV+SFGVVL EL+SS  A
Sbjct: 500 VADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYRLNEKSDVYSFGVVLSELISSKEA 559

Query: 358 VDITRHRQEINLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMK 414
           VDITRHR +INL NMAI+KIQ+ A+HEL D SLGF  D  +K M++SVAE+AF+CLQ  +
Sbjct: 560 VDITRHRHDINLANMAISKIQNDAVHELADLSLGFARDPSVKKMMSSVAELAFRCLQQER 619

BLAST of Carg15159-RA vs. TAIR 10
Match: AT1G66880.1 (Protein kinase superfamily protein )

HSP 1 Score: 424.9 bits (1091), Expect = 1.1e-118
Identity = 226/401 (56.36%), Postives = 282/401 (70.32%), Query Frame = 0

Query: 48   PPPP----DNSDDIKKKIIIGTCTGFGALLLTLLVLGICYRWR------QLRRKRSHMLP 107
            PP P    D S  I  K  I   +  G  +L L  L +C R R      Q   K   +  
Sbjct: 856  PPNPTRNKDTSLSIGAKAGIAVASVSGLAILLLAGLFLCIRRRRKTQDAQYTSKSLPITS 915

Query: 108  YVQRSVLSNP--------------PSVEEAENGGTYLGVHLFSYKELEEATNHFDSNKEL 167
            Y  R    NP              PS+    N   Y GV +FSY+ELEEAT +F  ++EL
Sbjct: 916  YSSRDTSRNPTSTTISSSSNHSLLPSISNLANRSDYCGVQVFSYEELEEATENF--SREL 975

Query: 168  GDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMNEVGILARLRHHNLVSLYGCTSSN 227
            GDGGFGTV+YG+LKDGRAVAVKRL+E + KRVEQF NE+ IL  L+H NLV LYGCTS +
Sbjct: 976  GDGGFGTVYYGVLKDGRAVAVKRLYERSLKRVEQFKNEIEILKSLKHPNLVILYGCTSRH 1035

Query: 228  SRELLLVYEYVPNGTVADHLHGKLAKPSKLPWSTRMKIAIETASALVYLHASEIIHRDVK 287
            SRELLLVYEY+ NGT+A+HLHG  A+   L WSTR+ IAIETASAL +LH   IIHRD+K
Sbjct: 1036 SRELLLVYEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASALSFLHIKGIIHRDIK 1095

Query: 288  TNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSF 347
            T NILLD++Y VKVADFGLSRLFP+D TH+STAPQGTPGYVDPEY+QCYQL++KSDV+SF
Sbjct: 1096 TTNILLDDNYQVKVADFGLSRLFPMDQTHISTAPQGTPGYVDPEYYQCYQLNEKSDVYSF 1155

Query: 348  GVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHALHELVDPSLGFESDDKIKDMITS 407
            GVVL EL+SS  AVDITRHR +INL NMA++KIQ++ALHELVD SLG+++D +++  + +
Sbjct: 1156 GVVLTELISSKEAVDITRHRHDINLANMAVSKIQNNALHELVDSSLGYDNDPEVRRKMMA 1215

Query: 408  VAEVAFQCLQSMKDERPTMMEVLDSLNAIQNRNGDAVQLRS 425
            VAE+AF+CLQ  +D RP M E+++ L  I++     V ++S
Sbjct: 1216 VAELAFRCLQQERDVRPAMDEIVEILRGIKDDEKKRVLVKS 1254

BLAST of Carg15159-RA vs. TAIR 10
Match: AT1G25390.1 (Protein kinase superfamily protein )

HSP 1 Score: 415.6 bits (1067), Expect = 6.5e-116
Identity = 213/353 (60.34%), Postives = 262/353 (74.22%), Query Frame = 0

Query: 63  IGTCTGFGALLLTLLVLGICYRWRQLRRKRSHMLPYVQRSVLSNPPSVEEAENGGTYLGV 122
           +G   G   +L+ +LV       R  RRK          S LS   S  + E    +  +
Sbjct: 225 LGLGIGGSVILIIILVALFAVIHRNYRRK--------DGSELSRDNSKSDVEFSQVFFKI 284

Query: 123 HLFSYKELEEATNHFDSNKELGDGGFGTVHYGLLKDGRAVAVKRLFESNFKRVEQFMNEV 182
            +FSYKEL+ AT++F  ++ LGDGGFGTV+YG ++DGR VAVKRL+E N++R+EQFMNE+
Sbjct: 285 PIFSYKELQAATDNFSKDRLLGDGGFGTVYYGKVRDGREVAVKRLYEHNYRRLEQFMNEI 344

Query: 183 GILARLRHHNLVSLYGCTSSNSRELLLVYEYVPNGTVADHLHGK-LAKPSKLPWSTRMKI 242
            IL RL H NLVSLYGCTS  SRELLLVYE++PNGTVADHL+G+       L WS R+ I
Sbjct: 345 EILTRLHHKNLVSLYGCTSRRSRELLLVYEFIPNGTVADHLYGENTPHQGFLTWSMRLSI 404

Query: 243 AIETASALVYLHASEIIHRDVKTNNILLDNHYSVKVADFGLSRLFPLDVTHVSTAPQGTP 302
           AIETASAL YLHAS+IIHRDVKT NILLD ++ VKVADFGLSRL P DVTHVSTAPQGTP
Sbjct: 405 AIETASALAYLHASDIIHRDVKTTNILLDRNFGVKVADFGLSRLLPSDVTHVSTAPQGTP 464

Query: 303 GYVDPEYHQCYQLSDKSDVFSFGVVLVELMSSMPAVDITRHRQEINLFNMAINKIQSHAL 362
           GYVDPEYH+CY L+DKSDV+SFGVVLVEL+SS PAVDI+R + EINL ++AINKIQ+HA 
Sbjct: 465 GYVDPEYHRCYHLTDKSDVYSFGVVLVELISSKPAVDISRCKSEINLSSLAINKIQNHAT 524

Query: 363 HELVDPSLGFESDDKIKDMITSVAEVAFQCLQSMKDERPTMMEVLDSLNAIQN 415
           HEL+D +LG+ +++ ++ M T VAE+AFQCLQ     RPTM +V+  L  IQN
Sbjct: 525 HELIDQNLGYATNEGVRKMTTMVAELAFQCLQQDNTMRPTMEQVVHELKGIQN 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023305.10.0e+00100.00LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2, par... [more]
KAG6589614.11.9e-24298.59LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2, par... [more]
XP_022921940.11.0e-24098.59LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 [Cuc... [more]
XP_022988480.17.2e-23495.56LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isof... [more]
XP_022988479.11.8e-23295.34LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isof... [more]
Match NameE-valueIdentityDescription
P0C5E27.2e-12858.23LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=A... [more]
Q8VYG03.0e-11860.21LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.3 OS=A... [more]
F4HQ176.7e-11856.71LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.4 OS=A... [more]
Q9C6K99.1e-11560.34LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.1 OS=A... [more]
Q8RY671.4e-7545.35Wall-associated receptor kinase-like 14 OS=Arabidopsis thaliana OX=3702 GN=WAKL1... [more]
Match NameE-valueIdentityDescription
A0A6J1E7705.0e-24198.59LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=C... [more]
A0A6J1JJN63.5e-23495.56LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isof... [more]
A0A6J1JLM78.6e-23395.34LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isof... [more]
A0A1S4DZK41.0e-18580.56LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 isof... [more]
A0A6J1C0J92.4e-17986.06LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.2 OS=M... [more]
Match NameE-valueIdentityDescription
AT1G18390.16.7e-12958.65Protein kinase superfamily protein [more]
AT1G18390.29.6e-12857.82Protein kinase superfamily protein [more]
AT5G38210.12.2e-11960.21Protein kinase family protein [more]
AT1G66880.11.1e-11856.36Protein kinase superfamily protein [more]
AT1G25390.16.5e-11660.34Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 137..412
e-value: 3.3E-39
score: 146.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 138..407
e-value: 1.2E-47
score: 162.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 137..412
score: 38.364635
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 214..432
e-value: 3.0E-54
score: 185.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 110..213
e-value: 2.3E-29
score: 103.5
NoneNo IPR availablePANTHERPTHR46008:SF2LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4coord: 55..424
IPR044652LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 1.1/1.2/1.3/1.4PANTHERPTHR46008LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4coord: 55..424
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 143..165
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 257..269
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 119..407

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg15159Carg15159gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg15159-RA:5Carg15159-RA:5exon
Carg15159-RA:4Carg15159-RA:4exon
Carg15159-RA:3Carg15159-RA:3exon
Carg15159-RA:2Carg15159-RA:2exon
Carg15159-RA:1Carg15159-RA:1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg15159-RA:cdsCarg15159-RA:cds_5CDS
Carg15159-RA:cdsCarg15159-RA:cds_4CDS
Carg15159-RA:cdsCarg15159-RA:cds_3CDS
Carg15159-RA:cdsCarg15159-RA:cds_2CDS
Carg15159-RA:cdsCarg15159-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg15159-RACarg15159-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity