Carg14035-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14035-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	UBA domain-containing protein
Location	Carg_Chr02: 6449857 .. 6452749 (+)
Sequence length	1261
RNA-Seq Expression	Carg14035-RA
Synteny	Carg14035-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTAAGATAAAATGGAAACCGCATATGAAATGACCATTTATAAAGTCTACTTCTCCGCGAAGCATTCAAGTTGGATTGTCCACCGTCTTTGCCTCTCCGTCTTCGACTGTACCGTCGTCTTCTTCCTCCTCCTTTCGGCTATGGCTTATGATTACAGAAACAAGCCCGGCTACTACAACGCTCATCCTCCGATGTACGGCCCAGCCGCTTCATCTTCACCATCTCCTTCAATTCATCCTATGTATACACAGTCCATGTACCCGAGGATCGGTCAGCAAGGTCATTCACCGGCGCCTCCGGTGGCTCGAGTCTCCTCCCACCACCATTCTTCTTCTGCTGCTCCTTCTCCATCTTCTCCCTCCTCTTGTAAGTTCTTCGCCTTTGATCTCGCTTATTTATGAGCTTTTGATGAATCGTTTAGGTTTGTGGTTTGCGAGTTTCAGAAATTGTTTTAGGGTTTGCTTGAATTGTTTAATCAACTCCTCCAATCTTGAGGTTTTTTGATTGTGATACGTTTTTTTTTTTCGTTTTGGTTTCGATTTGTTGATTGTACAGCAGGATTGGGCATCCGGGTTACCATTAAACCGGAATATCGAATTACTCCTCCGGTGAGTTTTGATTTTGAGTTTCATCGATTTCTGAATTGAATCGAGATATGATCGACTTTCCGGTTATTAAATCTGGTAATGGAAATGTTTGCTTCGGATTTTAATGGATCGATTATTTTCTTTTAGCCTCAATTATCGCCACAAGTTGGAGATATTCCTCGGAGCAATTTCCAATTTGATTTTGAGTTTGAGAAAAAGGTTTTAGCTGAAGCAGAGAAAGAAACTCCGAATTGGAATCGGTTTGGGTTGGAACACCCTCCTTCTAGACCAGTAGAGTCATCATCTTCAATGGTACCTTCTTTTCCTTCTTATGTAACGTTCTTTTTTTCTTCATCAAATAGATTCATAATGTAACTTTAGCCTTTTAACCAGGGATTAATCTAGGAAAATTTCATTTACCCAAAACGTATTTGTAACTCTTAATCCGTCTTGGGCTGATTTCTTTCCTTGATATCATGTATCATATATATTATTCCTTGGAAATTTTATTTATTTATTAATTTTGTACTTTGTGTTAATTGTCTCTTGAAAAAGAAAGATTATCTAGTCGATACTAGATACTATTTTGATTACCTTCTTCCTAAAATGTTATTATATGGTGTCTTCACGGCTTCGGTTCCCTCTTTTCCGTTTGGATGTCTACATATTTCCATAATCTATCATCCTCGTTGTTATGAGTATACATGTTTCGTTGTAGGGTTCTCCAGGAGATCCAGTTGTGAGCAAGTATGTTGCATCTGGCTTGAGTCGGGAAGCAGTTTCACTTGCTGTTGCTAATTATGGAGACAATCCAACCAAGGTAACTATGAATGATTGATGGAGTTACGTCCAAGGGGATGGGATCTGATCTTATTGTAATGTATTGTATTTATGGCATTTGATATATACTTGATAAACCACCATGACTGACATAGGGCTTAGTCCAAAATGGCCAATGTAAATAATAAAATACTTAAAAGAGAACGGATTCAAGCCATTTGTGGCCACCTGCCTAGGATTTAATGACCTACAAATTCTCTTGTCAAACAAATATTGTAGAGTTAGATAGTCGTGTCTCATAAGAATAGTTAATTTATATGTAGTCCATTTATACAAAATTGTGAAGGGGTCCTCTTGATTGAGAGGGATATCCTTCGGAGCATAAATAGAGGTGATGCATGGTTGTTGTCAGCTCGTGTTGTAAGGTGTTAGGTTAAGTCCCTCAATGAGCCTAACCCTCTTGTTTATTTGCCACCATTGAGGTTGGAACCTTGAGCCAATTACCAATGGTAAGCTAGGGGAAGGTGAGAATAAAGTCAAGTCATTGTGCCGTTTGTCCCCTAGGGACACATGCTACAATGGTTGGGATATATATGTGTATTCTTGATCATGTGTAAAGGGCTTGCAGGATTATCTTTACTTCTCCATGTACTATAATCAAGTTGCTCGTTTGCTTGCGATGCACAGATGGTGGATCTATCCACTCTTTGATGTTATCTTATAACTTATGCTATTTAATTTAATTTAATTTTACCTCACCTTTTATAAGGTTGAATATAAGCAATGTTACCTCACCTTTTAAGAGGTTAAAATATAGTCTTGTTTTCGTTCTTATTTAGTTCCAAACTCTAATAGAGTCCTGATTTAGTTCCTCTATTCCATCTCGTGAAGTACTCTTTTGTTTGTTTTTTTGGTTTGGATTTGCTGACTGACTCGAGCATAATTTTGTAGGTTCAAGAATTTGTGAAAGGCTACACACTCCTACGAGAGATGGGATTTCCATCCAACAAAGTGGTCGAGGCATTACTCATGTATGACAACGACACTGATAAGGCTGTAGCTCACTTTCTTGGCGGTACGTCATAAAATAAGAATTCTGGAAATGATTGTGTCAAGAATTGTGTTGTACTTCCAACTATGGCATCTTTGTATATGACAACAGAGGCCACTTGAGATTGTAAATTTGACAAAGAATATAGGCGTTTGTAGTGCTGACTATCTGGTTTTGGTTGATATTTTGGGAGCAGTATGATATTATCCATATGTTATACCAAAGTTATCACCTTTCTAGTAATGATTATGTATGGAATTTTGTTTTTGAACTTGTTTGATAATATTACCAGATAAACTGTGACAAACTCATACTGAGTTCTTCATGCATGAATTTCCCTTCAAGAGGGGGGAATCTTGAAGCAAAGAAACATGCTTATGACATAAAATAGTTGCTGGAATTCAGTTTAAGCTCTGTACAATACGAAAGGCAGATGCTCAATTGTAGCTTACAAATCGAGCATAATAGTTTCC

mRNA sequence

AAATTAAGATAAAATGGAAACCGCATATGAAATGACCATTTATAAAGTCTACTTCTCCGCGAAGCATTCAAGTTGGATTGTCCACCGTCTTTGCCTCTCCGTCTTCGACTGTACCGTCGTCTTCTTCCTCCTCCTTTCGGCTATGGCTTATGATTACAGAAACAAGCCCGGCTACTACAACGCTCATCCTCCGATGTACGGCCCAGCCGCTTCATCTTCACCATCTCCTTCAATTCATCCTATGTATACACAGTCCATGTACCCGAGGATCGGTCAGCAAGGTCATTCACCGGCGCCTCCGGTGGCTCGAGTCTCCTCCCACCACCATTCTTCTTCTGCTGCTCCTTCTCCATCTTCTCCCTCCTCTTCAGGATTGGGCATCCGGGTTACCATTAAACCGGAATATCGAATTACTCCTCCGCCTCAATTATCGCCACAAGTTGGAGATATTCCTCGGAGCAATTTCCAATTTGATTTTGAGTTTGAGAAAAAGGTTTTAGCTGAAGCAGAGAAAGAAACTCCGAATTGGAATCGGTTTGGGTTGGAACACCCTCCTTCTAGACCAGTAGAGTCATCATCTTCAATGGGTTCTCCAGGAGATCCAGTTGTGAGCAAGTATGTTGCATCTGGCTTGAGTCGGGAAGCAGTTTCACTTGCTGTTGCTAATTATGGAGACAATCCAACCAAGGTTCAAGAATTTGTGAAAGGCTACACACTCCTACGAGAGATGGGATTTCCATCCAACAAAGTGGTCGAGGCATTACTCATGTATGACAACGACACTGATAAGGCTGTAGCTCACTTTCTTGGCGGTACGTCATAAAATAAGAATTCTGGAAATGATTGTGTCAAGAATTGTGTTGTACTTCCAACTATGGCATCTTTGTATATGACAACAGAGGCCACTTGAGATTGTAAATTTGACAAAGAATATAGGCGTTTGTAGTGCTGACTATCTGGTTTTGGTTGATATTTTGGGAGCAGTATGATATTATCCATATGTTATACCAAAGTTATCACCTTTCTAGTAATGATTATGTATGGAATTTTGTTTTTGAACTTGTTTGATAATATTACCAGATAAACTGTGACAAACTCATACTGAGTTCTTCATGCATGAATTTCCCTTCAAGAGGGGGGAATCTTGAAGCAAAGAAACATGCTTATGACATAAAATAGTTGCTGGAATTCAGTTTAAGCTCTGTACAATACGAAAGGCAGATGCTCAATTGTAGCTTACAAATCGAGCATAATAGTTTCC

Coding sequence (CDS)

ATGGAAACCGCATATGAAATGACCATTTATAAAGTCTACTTCTCCGCGAAGCATTCAAGTTGGATTGTCCACCGTCTTTGCCTCTCCGTCTTCGACTGTACCGTCGTCTTCTTCCTCCTCCTTTCGGCTATGGCTTATGATTACAGAAACAAGCCCGGCTACTACAACGCTCATCCTCCGATGTACGGCCCAGCCGCTTCATCTTCACCATCTCCTTCAATTCATCCTATGTATACACAGTCCATGTACCCGAGGATCGGTCAGCAAGGTCATTCACCGGCGCCTCCGGTGGCTCGAGTCTCCTCCCACCACCATTCTTCTTCTGCTGCTCCTTCTCCATCTTCTCCCTCCTCTTCAGGATTGGGCATCCGGGTTACCATTAAACCGGAATATCGAATTACTCCTCCGCCTCAATTATCGCCACAAGTTGGAGATATTCCTCGGAGCAATTTCCAATTTGATTTTGAGTTTGAGAAAAAGGTTTTAGCTGAAGCAGAGAAAGAAACTCCGAATTGGAATCGGTTTGGGTTGGAACACCCTCCTTCTAGACCAGTAGAGTCATCATCTTCAATGGGTTCTCCAGGAGATCCAGTTGTGAGCAAGTATGTTGCATCTGGCTTGAGTCGGGAAGCAGTTTCACTTGCTGTTGCTAATTATGGAGACAATCCAACCAAGGTTCAAGAATTTGTGAAAGGCTACACACTCCTACGAGAGATGGGATTTCCATCCAACAAAGTGGTCGAGGCATTACTCATGTATGACAACGACACTGATAAGGCTGTAGCTCACTTTCTTGGCGGTACGTCATAA

Protein sequence

METAYEMTIYKVYFSAKHSSWIVHRLCLSVFDCTVVFFLLLSAMAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSHHHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLAEAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNPTKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS

Homology

[Close]

BLAST of Carg14035-RA vs. NCBI nr
Match: KAG7035731.1 (hypothetical protein SDJN02_02529 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541.2 bits (1393), Expect = 5.1e-150
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   METAYEMTIYKVYFSAKHSSWIVHRLCLSVFDCTVVFFLLLSAMAYDYRNKPGYYNAHPP 60
           METAYEMTIYKVYFSAKHSSWIVHRLCLSVFDCTVVFFLLLSAMAYDYRNKPGYYNAHPP
Sbjct: 1   METAYEMTIYKVYFSAKHSSWIVHRLCLSVFDCTVVFFLLLSAMAYDYRNKPGYYNAHPP 60

Query: 61  MYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSHHHSSSAAPSPSSPSSSG 120
           MYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSHHHSSSAAPSPSSPSSSG
Sbjct: 61  MYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSHHHSSSAAPSPSSPSSSG 120

Query: 121 LGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLAEAEKETPNWNRFGLEHP 180
           LGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLAEAEKETPNWNRFGLEHP
Sbjct: 121 LGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLAEAEKETPNWNRFGLEHP 180

Query: 181 PSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNPTKVQEFVKGYTLLREMG 240
           PSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNPTKVQEFVKGYTLLREMG
Sbjct: 181 PSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNPTKVQEFVKGYTLLREMG 240

Query: 241 FPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           FPSNKVVEALLMYDNDTDKAVAHFLGGTS
Sbjct: 241 FPSNKVVEALLMYDNDTDKAVAHFLGGTS 269

[Close]

BLAST of Carg14035-RA vs. NCBI nr
Match: KAG6605766.1 (Ubiquitin-conjugating enzyme E2 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 535.8 bits (1379), Expect = 2.1e-148
Identity = 266/267 (99.63%), Postives = 267/267 (100.00%), Query Frame = 0


Query: 1   METAYEMTIYKVYFSAKHSSWIVHRLCLSVFDCTVVFFLLLSAMAYDYRNKPGYYNAHPP 60
           METAYEMTIYKV+FSAKHSSWIVHRLCLSVFDCTVVFFLLLSAMAYDYRNKPGYYNAHPP
Sbjct: 1   METAYEMTIYKVHFSAKHSSWIVHRLCLSVFDCTVVFFLLLSAMAYDYRNKPGYYNAHPP 60

Query: 61  MYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSHHHSSSAAPSPSSPSSSG 120
           MYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSHHHSSSAAPSPSSPSSSG
Sbjct: 61  MYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSHHHSSSAAPSPSSPSSSG 120

Query: 121 LGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLAEAEKETPNWNRFGLEHP 180
           LGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLAEAEKETPNWNRFGLEHP
Sbjct: 121 LGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLAEAEKETPNWNRFGLEHP 180

Query: 181 PSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNPTKVQEFVKGYTLLREMG 240
           PSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNPTKVQEFVKGYTLLREMG
Sbjct: 181 PSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNPTKVQEFVKGYTLLREMG 240

Query: 241 FPSNKVVEALLMYDNDTDKAVAHFLGG 268
           FPSNKVVEALLMYDNDTDKAVAHFLGG
Sbjct: 241 FPSNKVVEALLMYDNDTDKAVAHFLGG 267

[Close]

BLAST of Carg14035-RA vs. NCBI nr
Match: XP_022958203.1 (uncharacterized protein LOC111459499 [Cucurbita moschata])

HSP 1 Score: 456.1 bits (1172), Expect = 2.2e-124
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 103
           MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH
Sbjct: 1   MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 60

Query: 104 HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 163
           HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA
Sbjct: 61  HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 120

Query: 164 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 223
           EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP
Sbjct: 121 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 180

Query: 224 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS
Sbjct: 181 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 226

[Close]

BLAST of Carg14035-RA vs. NCBI nr
Match: XP_023534738.1 (uncharacterized protein LOC111796216 [Cucurbita pepo subsp. pepo] >XP_023534739.1 uncharacterized protein LOC111796216 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454.9 bits (1169), Expect = 4.8e-124
Identity = 225/226 (99.56%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 103
           MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH
Sbjct: 1   MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 60

Query: 104 HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 163
           HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA
Sbjct: 61  HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 120

Query: 164 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 223
           EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVV+KYVASGLSREAVSLAVANYGDNP
Sbjct: 121 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVNKYVASGLSREAVSLAVANYGDNP 180

Query: 224 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS
Sbjct: 181 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 226

[Close]

BLAST of Carg14035-RA vs. NCBI nr
Match: XP_022995350.1 (velvet complex subunit B [Cucurbita maxima])

HSP 1 Score: 453.0 bits (1164), Expect = 1.8e-123
Identity = 224/226 (99.12%), Postives = 225/226 (99.56%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 103
           MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPV RVSSH
Sbjct: 1   MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVPRVSSH 60

Query: 104 HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 163
           HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA
Sbjct: 61  HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 120

Query: 164 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 223
           EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVV+KYVASGLSREAVSLAVANYGDNP
Sbjct: 121 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVNKYVASGLSREAVSLAVANYGDNP 180

Query: 224 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS
Sbjct: 181 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 226

[Close]

BLAST of Carg14035-RA vs. ExPASy TrEMBL
Match: A0A6J1H2H7 (uncharacterized protein LOC111459499 OS=Cucurbita moschata OX=3662 GN=LOC111459499 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.0e-124
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 103
           MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH
Sbjct: 1   MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 60

Query: 104 HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 163
           HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA
Sbjct: 61  HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 120

Query: 164 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 223
           EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP
Sbjct: 121 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 180

Query: 224 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS
Sbjct: 181 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 226

[Close]

BLAST of Carg14035-RA vs. ExPASy TrEMBL
Match: A0A6J1K3U0 (velvet complex subunit B OS=Cucurbita maxima OX=3661 GN=LOC111490923 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 8.8e-124
Identity = 224/226 (99.12%), Postives = 225/226 (99.56%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 103
           MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPV RVSSH
Sbjct: 1   MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVPRVSSH 60

Query: 104 HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 163
           HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA
Sbjct: 61  HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 120

Query: 164 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 223
           EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVV+KYVASGLSREAVSLAVANYGDNP
Sbjct: 121 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVNKYVASGLSREAVSLAVANYGDNP 180

Query: 224 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS
Sbjct: 181 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 226

[Close]

BLAST of Carg14035-RA vs. ExPASy TrEMBL
Match: A0A0A0KNB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139700 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.5e-102
Identity = 194/226 (85.84%), Postives = 205/226 (90.71%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMYTQSMYPRIGQQGHSPAPPVARVSSH 103
           MAYD+RN  G+Y++H PMY   ASSSPSPS HPMY+ SMYPRIGQQ  S  PPVAR+SSH
Sbjct: 1   MAYDFRNNSGHYDSHQPMYTSTASSSPSPSPHPMYSHSMYPRIGQQAPSSTPPVARLSSH 60

Query: 104 HHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 163
           H+SSS +PSPS  SSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA
Sbjct: 61  HYSSSPSPSPS--SSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKVLA 120

Query: 164 EAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGDNP 223
           EAEKE PNWNRFGLEHPP +PVES+SSMGS GDPVVSKYVASGL+REAVS AVANYGDNP
Sbjct: 121 EAEKEAPNWNRFGLEHPPPKPVESTSSMGSIGDPVVSKYVASGLNREAVSFAVANYGDNP 180

Query: 224 TKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           TKVQEFVKGYTLLREMGF S KVVEALLMYDNDTDKAVAHFLGGTS
Sbjct: 181 TKVQEFVKGYTLLREMGFSSIKVVEALLMYDNDTDKAVAHFLGGTS 224

[Close]

BLAST of Carg14035-RA vs. ExPASy TrEMBL
Match: A0A6J1DVL2 (uncharacterized protein LOC111023918 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023918 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.6e-101
Identity = 188/228 (82.46%), Postives = 206/228 (90.35%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMY--TQSMYPRIGQQGHSPAPPVARVS 103
           MAYDYRNKPG+Y++HPPMYGP AS+SPSPS+HPMY  + S+YPRIGQQGHSPAPPVAR+S
Sbjct: 1   MAYDYRNKPGHYDSHPPMYGPPASASPSPSLHPMYGHSMSLYPRIGQQGHSPAPPVARIS 60

Query: 104 SHHHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKV 163
           SHHHSS    SPS PSSSGLGI VTIKPEYRITPPPQLSPQ+ DIPRSNFQFDFEFEKKV
Sbjct: 61  SHHHSS----SPSPPSSSGLGIGVTIKPEYRITPPPQLSPQIRDIPRSNFQFDFEFEKKV 120

Query: 164 LAEAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGD 223
           LAEAEKET NW+RFGL++ P++P E +S+MGS GDPV SKY+ASGL+REAV  AVANYGD
Sbjct: 121 LAEAEKETQNWSRFGLDNLPAKPGEPTSTMGSLGDPVASKYIASGLNREAVRFAVANYGD 180

Query: 224 NPTKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           NPTKVQEF KGYTLLREMGF SNKVVEALLMYDNDTDKAVAHFL GTS
Sbjct: 181 NPTKVQEFAKGYTLLREMGFSSNKVVEALLMYDNDTDKAVAHFLSGTS 224

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BLAST of Carg14035-RA vs. ExPASy TrEMBL
Match: A0A6J1DSJ8 (uncharacterized protein LOC111023918 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023918 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.0e-100
Identity = 186/226 (82.30%), Postives = 204/226 (90.27%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSIHPMY--TQSMYPRIGQQGHSPAPPVARVS 103
           MAYDYRNKPG+Y++HPPMYGP AS+SPSPS+HPMY  + S+YPRIGQQGHSPAPPVAR+S
Sbjct: 1   MAYDYRNKPGHYDSHPPMYGPPASASPSPSLHPMYGHSMSLYPRIGQQGHSPAPPVARIS 60

Query: 104 SHHHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFEKKV 163
           SHHHSS    SPS PSSSGLGI VTIKPEYRITPPPQLSPQ+ DIPRSNFQFDFEFEKKV
Sbjct: 61  SHHHSS----SPSPPSSSGLGIGVTIKPEYRITPPPQLSPQIRDIPRSNFQFDFEFEKKV 120

Query: 164 LAEAEKETPNWNRFGLEHPPSRPVESSSSMGSPGDPVVSKYVASGLSREAVSLAVANYGD 223
           LAEAEKET NW+RFGL++ P++P E +S+MGS GDPV SKY+ASGL+REAV  AVANYGD
Sbjct: 121 LAEAEKETQNWSRFGLDNLPAKPGEPTSTMGSLGDPVASKYIASGLNREAVRFAVANYGD 180

Query: 224 NPTKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGG 268
           NPTKVQEF KGYTLLREMGF SNKVVEALLMYDNDTDKAVAHFL G
Sbjct: 181 NPTKVQEFAKGYTLLREMGFSSNKVVEALLMYDNDTDKAVAHFLSG 222

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BLAST of Carg14035-RA vs. TAIR 10
Match: AT5G53330.1 (Ubiquitin-associated/translation elongation factor EF1B protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-64
Identity = 139/234 (59.40%), Postives = 167/234 (71.37%), Query Frame = 0


Query: 44  MAYDYRNKPGYYNAHPPMYGPAASSSPSPSI-HPMYTQSMYPRIGQQGHSPAP----PVA 103
           M YDYRNK G  +   PMYGP  S+SPSPS  HPMY    YP+IGQQ   P P    P  
Sbjct: 1   MDYDYRNKSGGPSYPRPMYGP-PSTSPSPSSNHPMYG---YPKIGQQ-TGPGPQFFSPPE 60

Query: 104 RVSSHHHSSSAAPSPSSPSSSGLGIRVTIKPEYRITPPPQLSPQVGDIPRSNFQFDFEFE 163
           R SS  H++S         SSG+GIRV +KPEYRITPPPQL P+VGDI RS+FQFDF  E
Sbjct: 61  RNSSFQHNTS--------PSSGIGIRVNLKPEYRITPPPQLLPRVGDIHRSSFQFDFGLE 120

Query: 164 KKVLAEAEKETPNWNRFGLEHPPSR---PVESSSSMGSPGDPVVSKYVASGLSREAVSLA 223
           +KVLAEAEK+ P+W++FG E+PP++   P  SS       D VV KY ASGL+REAV++A
Sbjct: 121 RKVLAEAEKDNPDWSKFGSENPPAKFHEPSPSSVGQMQGVDHVVMKYTASGLNREAVNIA 180

Query: 224 VANYGDNPTKVQEFVKGYTLLREMGFPSNKVVEALLMYDNDTDKAVAHFLGGTS 270
           VANYGDNPTKVQEF  G+T +REMGFP+N V +AL M++NDTDKA+AH L G+S
Sbjct: 181 VANYGDNPTKVQEFANGFTAIREMGFPTNAVADALFMFENDTDKALAHLLHGSS 221

The following BLAST results are available for this feature:

BLAST of Carg14035-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035731.1	5.1e-150	100.00	hypothetical protein SDJN02_02529 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605766.1	2.1e-148	99.63	Ubiquitin-conjugating enzyme E2 10, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022958203.1	2.2e-124	100.00	uncharacterized protein LOC111459499 [Cucurbita moschata]	[more]
XP_023534738.1	4.8e-124	99.56	uncharacterized protein LOC111796216 [Cucurbita pepo subsp. pepo] >XP_023534739....	[more]
XP_022995350.1	1.8e-123	99.12	velvet complex subunit B [Cucurbita maxima]	[more]

BLAST of Carg14035-RA vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg14035-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H2H7	1.0e-124	100.00	uncharacterized protein LOC111459499 OS=Cucurbita moschata OX=3662 GN=LOC1114594...	[more]
A0A6J1K3U0	8.8e-124	99.12	velvet complex subunit B OS=Cucurbita maxima OX=3661 GN=LOC111490923 PE=4 SV=1	[more]
A0A0A0KNB8	1.5e-102	85.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139700 PE=4 SV=1	[more]
A0A6J1DVL2	3.6e-101	82.46	uncharacterized protein LOC111023918 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DSJ8	4.0e-100	82.30	uncharacterized protein LOC111023918 isoform X1 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Carg14035-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53330.1	1.2e-64	59.40	Ubiquitin-associated/translation elongation factor EF1B protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042575	Ubiquitin-associated protein 1, C-terminal	GENE3D	1.20.120.1920	UBAP1 SOUBA domain	coord: 193..267 e-value: 4.0E-7 score: 32.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..194
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 101..121
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..121
None	No IPR available	PANTHER	PTHR15960:SF7	SUBFAMILY NOT NAMED	coord: 44..267
None	No IPR available	CDD	cd14316	UBA2_UBAP1_like	coord: 229..265 e-value: 1.25585E-8 score: 47.9077
IPR038870	Ubiquitin-associated protein 1	PANTHER	PTHR15960	LD44032P	coord: 44..267
IPR015940	Ubiquitin-associated domain	PROSITE	PS50030	UBA	coord: 225..267 score: 8.811843
IPR009060	UBA-like superfamily	SUPERFAMILY	46934	UBA-like	coord: 225..267

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg14035	Carg14035	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14035-RA:1	Carg14035-RA:1	exon
Carg14035-RA:2	Carg14035-RA:2	exon
Carg14035-RA:3	Carg14035-RA:3	exon
Carg14035-RA:4	Carg14035-RA:4	exon
Carg14035-RA:5	Carg14035-RA:5	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14035-RA:five_prime_utr	Carg14035-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14035-RA:cds	Carg14035-RA:cds	CDS
Carg14035-RA:cds	Carg14035-RA:cds_2	CDS
Carg14035-RA:cds	Carg14035-RA:cds_3	CDS
Carg14035-RA:cds	Carg14035-RA:cds_4	CDS
Carg14035-RA:cds	Carg14035-RA:cds_5	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14035-RA:three_prime_utr	Carg14035-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg14035-RA	Carg14035-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043162	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component	GO:0000813	ESCRT I complex
molecular_function	GO:0005515	protein binding
molecular_function	GO:0043130	ubiquitin binding