Carg14010-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14010-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	AB hydrolase-1 domain-containing protein
Location	Carg_Chr02: 6580776 .. 6584866 (-)
Sequence length	2225
RNA-Seq Expression	Carg14010-RA
Synteny	Carg14010-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTCCAAAATCCCCAATTTTCTTCTTCGTCTTCTTCATCTGCTTCCAAATATGCCGTTCATTGAGATTAACTCCAGCACCACAGGCGCGGAGGACGCCAAGATCAACACAGCAATCAAAATTTTCTACAGAACTTACGGACGCGGATCAACCAAGGTCCTCCTCATAATAGGTCTCTTCAAGATTGCTCCATTTTTCCGTTTTTCCCCTTTCGATTTCTGCTTTTTTCGAATTTCATTCTGATAGCGCTTAAATGGATGAATCAATAATGGATAGGATTGGCCGGAACCCACGATTCATGGAACCCCCAAATCAAAGGGCTTACAGGCACCGATGTGCCGAATGACCACGGCAATCGGAACACCGTCGGTAACCAGATTGCGGCGGACGACGGCGAAGAAGCCAGCTTCGAAGATGGCGGAGGAATCGAAGTTTGTGCATTTGACAATCGGGGAATGGGCCGAAGCTCCGCCCCCACCAAAAAATCTGAATATACGTGAGTTCCACAATCTATTACTTATGAATCACTTCTCTCAATCTCTCTTATCTTAGTTGCTTGTCTTGAGAAATCAATTAGAATTCAGGATTTAAGCCAATTTTCCAATACCCTGAGCGAGAGAGAAAGAAAAGGATAAACCCCTGTTTTTTTCTTCTTGGTTGTGCAGAACAAAGATAATGGCTCAGGATGCCATTGCCTTATTGGATCATTTGGGATGGGAAAAGGTCCATGTCTTTGGTCATTCCATGGGTGAGTGAGTGAGTTTGTAACTCTGCTCAATGATTCATGAACAAGATATAGTTATTAATGTTTTGTTCTATCAATAGGAGGTATGATAGCTTGCAAATTAGCTGCTATTGCGCCTGAAAGAGTCAAGTCATTGGCCATGCTCAATGTCACTGGTGGAGGCTTTCAATGTTGCCCCAAGGTACCTTAATAAACATTTAGTGCTGCCACTCTTAGCTGCTTGTTTGTAACATTATCTTCAGAAAACATTACATTACTTAAGGAATCCAACCAAAATGCTGTTCTGTTGTGGTTGAGCTGGTTCTTAAGGAACAAATTGTTCTTGTAGCTTGACAGGCAAACATTCGACATTGCAGTCCGGTTCTTAAAGGCCAAGACTCCTGAACAAAGGGCAAGTGTTGATTTGGATACACACTATTCAAAGGTAAAGTTAATCATCATGTTCGTCTTCTGTTATCTGCGACCCGACTTTAAACGAGGATGAGTCGATGAATAGTTTTAACAAAATTTGTATATTGTGGTGCAGGAATATCTTGAGGAGTTCGTCGGATTTGAAACAAGAAGAGCGGTATTATATCGGGTATGTAATGATGGGAGGATCATTCTTAACTGGTTTTATGTATACCTTGTTAGTTGGATTCTGGTTTTTATTAAACCTGCTCGTATCCCGTTCCCGCTCCCACTCCGTCTTTTCTAGCTGCTCTCTCCTCTTGAAACCTCCCCAAAAAGGAGGGAATATGGGAGCTAGAAGTGTCTTTTTTTATGGATTCTGAAGCTTACCTTGTTGGGTTATAGTTTTCTTTGTCAACTAGTTTGGAACTTTACAACATTCCATCACAAACATATGCATACATGTTCGGTGATACCGATATATATCCACGTTCACAGAAACGAAAGTAAACCATGATATTACCTGACAATAGCCAAGTGTTTATGGCAATGAGCGTCGTGTATGGAATCTAATCATGAATGAAAAGGAGGAGTCCGACATCCACGAACCCTCTTACAAGAAAATTCTTTTGAACTTCCTTATGAAAATGACTTTAACCTAGAGGAATAAGTAAGACAAAGAACCAATGGCTGTTGTAGTAGGGGTCTGATTGAGTCTCAACTCGTGAGCAAAACTAATCCCTTCTCAAATTGCCGAAGTCGGTCGTGATCGACATGTAATCCTTTCCTTGAGGTTGGAGGATCGAATCGACATGCCCCACTTTGTTGTACTAAAAAATAATAATAATCCCATCCCGAACATGTTAATATATGTATGCCTAGATAGCACTCTGCCAAAATGAATGGATTTTGTTTAAACCATGAGCATATATTGAGAGACTTCTGAATGTTTGAAAAACTTATTAAGAACTAAAGGTGTAGTTGATTCTATGACACTATCTTCATATGTTGATTGTTTATATCAATGCCTCAACAGCTCTGGTCAATCATTACAGACGCATACACATAAAGACATACTTTAAGCTTTAGAGCCTTAGTGAACTCGAAACAATCATTTGCAGGAATATGTTAAAGGTATATCAGCAACAGGAATGCAGACTGATTATGGTTTTGCCGGTCAAGTCAATGCATGCTGGACGCATAAAGTAACGAACAAAGAGATAGAATGCCTTCAATCTGCTGGATTTCTTGTATCAGTCATTCATGGAAGGTAAACGATCACGTCAGTTAATCGCTACGAAACTTATGGCTGACATCCAAATCTGTATGAACATAAACATGTTTCTATCATCTAACATTTGCTGCATCAACATACTTTACGAACGATGATTGGTTTTCGATGATCAGGCAAGATGTTATTGCTCAAATATACTATGCTAGAAAACTTGCAGAGAAGCTTTATCCTGTTGCTAGAATGGTAGATCTTCATGGAGGTCATTTAGTCAGTCGAGAGAGAACTGAAGAGGTATAACTTTTTCTTAATCAGAATCGATCGAAACCGTGTTTTTCACTAGAAACTGATAGACATTTCTATTTTTTTGTGTGAAAGTTCAGGTAAATCAAGCTCTTCTTGACTTAATCAGAGCTTCAGAAACGAAGATGAGTCCACAGGACTGGACAAACTTGCCAAAGAAGAGCTCATGTGAGCATTTTTTTTTTCTTTTAGCAGTCAACTTTTTTCTCTTTCGTTCCTGGGGTCCCACATTAGTTGGGGAGGAGAACAAAACACTCTTTATAAGGGTGTGGAAACCTTTCCCTTGCAAACATGTTTTAAAAACTTTGAGGAGAAACTCGAAAGGGAAAGTCCAAAGAGGACAGTATCTGCTAGCTGTGGGCATTGGGCCGTTACAATAACTTGGAAATGCTGAAAAATCCGTTGTTTCTAATAGGGTGGATGGAGGAGAGAATGTCATTAATAACACTAAAAACTGAAGGAGGAGGAAGCAATGTCTCCACCAAACCTTTCTTGTTGGAGAGACTGCATCTTTTCTTCCTGTATCTCTTCGGTCTGATTCTTTTCATGTTCGAGTATCTCCGGGACACCTTCAAAAGCTTGAAACCAGCTCGAGTTGGGCCCTCCCTTACGTGAAGAATCGTTCCAAAGACTAAATTTTTACCAGTGAGTTATCTGATTGTCCCAACAACGGTCATGTAAATAATATAAAAACTTTTGAAAATAGTTAAGGTGCGTTCAAGCTGGTTTAGACACGCATAAAGATTAAAAAAACGATAGCCCTTTACTTCCATTGAACTCGAACTATAAGAGGTCTCAAGTTTGTTCTTTGCTTCAACTTTGTTGCAGGGTCTGTTCATCTTTGCTATCTGATGGGCAATGATCCAATCATCAAAGTGAAGATGGGGAATGAGTTCTCTTGCAATATTGAGAAATTATATCAACAAAGGAGATAATCCAACAAAAGGCCAATGTTAAAGCCTGGTATACCCTCCCTTCTTCTGTATAATCTATAAAACTGTTCATCCTGTTCATCTTATACCACTAAATGCATATTATTCTCTGTTTAAAATTCGTTTTATAGTTCATCTATTCAAGATATTGAAAGTTTAGCGTCGGTCAAACCTATTCCTAAGAAAACCAAATAGAGATCGATTCTAATATCATTAAGAGCACTGCATAGATAAGCTCATACTAACCCGTCAATTTTGGATCTAATTCGGAATTGAGTTGCCGGAAAGTCGTGGCAGAGGAGGTTGCCTTCATAAGATGGCTGCTTTGCAAGATGGAATCTAAAAGAATGAGAGAAGATGAACTCAAATTGTGGATTTGGAGAGAAATTTAAGCAATAGGACCAAAATATGCCTTTGGAATATACCAATATGCTCCCATGCTACCCTACATGACAAAATATGCCTTGGGTTGAAGCCCAGTTGAACCAG

mRNA sequence

AATTTCCAAAATCCCCAATTTTCTTCTTCGTCTTCTTCATCTGCTTCCAAATATGCCGTTCATTGAGATTAACTCCAGCACCACAGGCGCGGAGGACGCCAAGATCAACACAGCAATCAAAATTTTCTACAGAACTTACGGACGCGGATCAACCAAGGTCCTCCTCATAATAGGATTGGCCGGAACCCACGATTCATGGAACCCCCAAATCAAAGGGCTTACAGGCACCGATGTGCCGAATGACCACGGCAATCGGAACACCGTCGGTAACCAGATTGCGGCGGACGACGGCGAAGAAGCCAGCTTCGAAGATGGCGGAGGAATCGAAGTTTGTGCATTTGACAATCGGGGAATGGGCCGAAGCTCCGCCCCCACCAAAAAATCTGAATATACAACAAAGATAATGGCTCAGGATGCCATTGCCTTATTGGATCATTTGGGATGGGAAAAGGTCCATGTCTTTGGTCATTCCATGGGAGGTATGATAGCTTGCAAATTAGCTGCTATTGCGCCTGAAAGAGTCAAGTCATTGGCCATGCTCAATGTCACTGGTGGAGGCTTTCAATGTTGCCCCAAGCTTGACAGGCAAACATTCGACATTGCAGTCCGGTTCTTAAAGGCCAAGACTCCTGAACAAAGGGCAAGTGTTGATTTGGATACACACTATTCAAAGGAATATCTTGAGGAGTTCGTCGGATTTGAAACAAGAAGAGCGGTATTATATCGGGAATATGTTAAAGGTATATCAGCAACAGGAATGCAGACTGATTATGGTTTTGCCGGTCAAGTCAATGCATGCTGGACGCATAAAGTAACGAACAAAGAGATAGAATGCCTTCAATCTGCTGGATTTCTTGTATCAGTCATTCATGGAAGGTAAACGATCACGTCAGTTAATCGCTACGAAACTTATGGCTGACATCCAAATCTGTATGAACATAAACATGTTTCTATCATCTAACATTTGCTGCATCAACATACTTTACGAACGATGATTGGTTTTCGATGATCAGGCAAGATGTTATTGCTCAAATATACTATGCTAGAAAACTTGCAGAGAAGCTTTATCCTGTTGCTAGAATGGTAGATCTTCATGGAGGTCATTTAGTCAGTCGAGAGAGAACTGAAGAGGTAAATCAAGCTCTTCTTGACTTAATCAGAGCTTCAGAAACGAAGATGAGTCCACAGGACTGGACAAACTTGCCAAAGAAGAGCTCATGGTGGATGGAGGAGAGAATGTCATTAATAACACTAAAAACTGAAGGAGGAGGAAGCAATGTCTCCACCAAACCTTTCTTGTTGGAGAGACTGCATCTTTTCTTCCTGTATCTCTTCGGTCTGATTCTTTTCATGTTCGAGTATCTCCGGGACACCTTCAAAAGCTTGAAACCAGCTCGAGTTGGGCCCTCCCTTACGTGAAGAATCGTTCCAAAGACTAAATTTTTACCAGTGAGTTATCTGATTGTCCCAACAACGGTCATGTAAATAATATAAAAACTTTTGAAAATAGTTAAGGTGCGTTCAAGCTGGTTTAGACACGCATAAAGATTAAAAAAACGATAGCCCTTTACTTCCATTGAACTCGAACTATAAGAGGTCTCAAGTTTGTTCTTTGCTTCAACTTTGTTGCAGGGTCTGTTCATCTTTGCTATCTGATGGGCAATGATCCAATCATCAAAGTGAAGATGGGGAATGAGTTCTCTTGCAATATTGAGAAATTATATCAACAAAGGAGATAATCCAACAAAAGGCCAATGTTAAAGCCTGGTATACCCTCCCTTCTTCTGTATAATCTATAAAACTGTTCATCCTGTTCATCTTATACCACTAAATGCATATTATTCTCTGTTTAAAATTCGTTTTATAGTTCATCTATTCAAGATATTGAAAGTTTAGCGTCGGTCAAACCTATTCCTAAGAAAACCAAATAGAGATCGATTCTAATATCATTAAGAGCACTGCATAGATAAGCTCATACTAACCCGTCAATTTTGGATCTAATTCGGAATTGAGTTGCCGGAAAGTCGTGGCAGAGGAGGTTGCCTTCATAAGATGGCTGCTTTGCAAGATGGAATCTAAAAGAATGAGAGAAGATGAACTCAAATTGTGGATTTGGAGAGAAATTTAAGCAATAGGACCAAAATATGCCTTTGGAATATACCAATATGCTCCCATGCTACCCTACATGACAAAATATGCCTTGGGTTGAAGCCCAGTTGAACCAG

Coding sequence (CDS)

ATGCCGTTCATTGAGATTAACTCCAGCACCACAGGCGCGGAGGACGCCAAGATCAACACAGCAATCAAAATTTTCTACAGAACTTACGGACGCGGATCAACCAAGGTCCTCCTCATAATAGGATTGGCCGGAACCCACGATTCATGGAACCCCCAAATCAAAGGGCTTACAGGCACCGATGTGCCGAATGACCACGGCAATCGGAACACCGTCGGTAACCAGATTGCGGCGGACGACGGCGAAGAAGCCAGCTTCGAAGATGGCGGAGGAATCGAAGTTTGTGCATTTGACAATCGGGGAATGGGCCGAAGCTCCGCCCCCACCAAAAAATCTGAATATACAACAAAGATAATGGCTCAGGATGCCATTGCCTTATTGGATCATTTGGGATGGGAAAAGGTCCATGTCTTTGGTCATTCCATGGGAGGTATGATAGCTTGCAAATTAGCTGCTATTGCGCCTGAAAGAGTCAAGTCATTGGCCATGCTCAATGTCACTGGTGGAGGCTTTCAATGTTGCCCCAAGCTTGACAGGCAAACATTCGACATTGCAGTCCGGTTCTTAAAGGCCAAGACTCCTGAACAAAGGGCAAGTGTTGATTTGGATACACACTATTCAAAGGAATATCTTGAGGAGTTCGTCGGATTTGAAACAAGAAGAGCGGTATTATATCGGGAATATGTTAAAGGTATATCAGCAACAGGAATGCAGACTGATTATGGTTTTGCCGGTCAAGTCAATGCATGCTGGACGCATAAAGTAACGAACAAAGAGATAGAATGCCTTCAATCTGCTGGATTTCTTGTATCAGTCATTCATGGAAGGTAA

Protein sequence

MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTDVPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQDAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDYGFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR

Homology

[Close]

BLAST of Carg14010-RA vs. NCBI nr
Match: KAG7035755.1 (rutD [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 563.1 bits (1450), Expect = 1.3e-156
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD
Sbjct: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. NCBI nr
Match: KAG6605790.1 (hypothetical protein SDJN03_03107, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 562.0 bits (1447), Expect = 2.8e-156
Identity = 274/275 (99.64%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIEIN+STTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. NCBI nr
Match: XP_023532415.1 (uncharacterized protein LOC111794595 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.5 bits (1443), Expect = 8.3e-156
Identity = 273/275 (99.27%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIEIN+STTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQ+DY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQSDY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. NCBI nr
Match: XP_022995986.1 (uncharacterized protein LOC111491334 [Cucurbita maxima])

HSP 1 Score: 558.9 bits (1439), Expect = 2.4e-155
Identity = 272/275 (98.91%), Postives = 274/275 (99.64%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIEIN+STTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPNDHGNRNTVGNQIAA DGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ
Sbjct: 61  VPNDHGNRNTVGNQIAAGDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKVH+FGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHIFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. NCBI nr
Match: XP_022958692.1 (uncharacterized protein LOC111459843 [Cucurbita moschata])

HSP 1 Score: 558.1 bits (1437), Expect = 4.1e-155
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIEIN+STTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWN QIKGLTGTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNLQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. ExPASy Swiss-Prot
Match: A8IAD8 (Putative aminoacrylate hydrolase RutD OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571) OX=438753 GN=rutD PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 9.0e-12
Identity = 52/171 (30.41%), Postives = 74/171 (43.27%), Query Frame = 0


Query: 24  IFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTDVPNDHGNRNTVGNQIAADDGEEA 83
           ++Y  +G G T +LL  G+ G    W PQI+ L                           
Sbjct: 11  LYYEIHGAG-TPILLSAGMGGGAGFWRPQIEALAARH----------------------- 70

Query: 84  SFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQDAIALLDHLGWEKVHVFGHSMGG 143
                   +V  +D+ G GRS         T   MA+D   +LD  G E  HV GH++GG
Sbjct: 71  --------QVILYDHAGTGRSGRDIGPRSITE--MARDMARVLDAAGVEDAHVAGHAIGG 130

Query: 144 MIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPE 195
           +I  +LA  APERV+SL ++N    G+       R+ F++  R L A  PE
Sbjct: 131 IIGMELALAAPERVRSLTIVN----GWARADGFLRRCFEVRKRILLASGPE 143

[Close]

BLAST of Carg14010-RA vs. ExPASy Swiss-Prot
Match: B7KWT4 (Putative aminoacrylate hydrolase RutD OS=Methylorubrum extorquens (strain CM4 / NCIMB 13688) OX=440085 GN=rutD PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.9e-10
Identity = 40/120 (33.33%), Postives = 59/120 (49.17%), Query Frame = 0


Query: 93  VCAFDNRGMGRSSAPTKKSEYTTKIMAQDAIALLDHLGWEKVHVFGHSMGGMIACKLAAI 152
           V  +D+RG GRS +P +   +    MA+D +ALLDHLG     + GH++GG+IA +LA  
Sbjct: 44  VVTYDHRGTGRSPSPLEPG-HDIAAMARDVLALLDHLGIGTADIVGHALGGLIALQLALT 103

Query: 153 APERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLEE 212
            PERV  + ++N    G+       R+ F      L+   PE          Y   +L E
Sbjct: 104 HPERVGRIVVIN----GWAAMDPATRRCFAARKALLRHAGPEAFVRAQAIFLYPAPWLSE 158

[Close]

BLAST of Carg14010-RA vs. ExPASy Swiss-Prot
Match: B1ZB18 (Putative aminoacrylate hydrolase RutD OS=Methylorubrum populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) OX=441620 GN=rutD PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 6.4e-10
Identity = 40/120 (33.33%), Postives = 57/120 (47.50%), Query Frame = 0


Query: 93  VCAFDNRGMGRSSAPTKKSEYTTKIMAQDAIALLDHLGWEKVHVFGHSMGGMIACKLAAI 152
           V  +D+RG GRS  P +   +    MA+D +ALLDHLG     + GH++GG+IA  LA  
Sbjct: 44  VVTYDHRGTGRSPGPLEPG-HDIAAMARDVLALLDHLGIGTTDIVGHALGGLIALHLALT 103

Query: 153 APERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLEE 212
            PERV+ + ++N    G+       R+ F      L    PE          Y   +L E
Sbjct: 104 HPERVERIVVIN----GWAVMDPATRRCFSARKALLTQAGPEAFVRAQAIFLYPAPWLSE 158

[Close]

BLAST of Carg14010-RA vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 4.2e-09
Identity = 29/75 (38.67%), Postives = 43/75 (57.33%), Query Frame = 0


Query: 90  GIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQDAIALLDHLGWEKVHVFGHSMGGMIACKL 149
           G  V A D +G G SSAP +  EY  +++ ++ +  LD LG  +    GH  GGM+   +
Sbjct: 285 GYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYM 344

Query: 150 AAIAPERVKSLAMLN 165
           A   PERV+++A LN
Sbjct: 345 ALFYPERVRAVASLN 359

[Close]

BLAST of Carg14010-RA vs. ExPASy Swiss-Prot
Match: C7CM33 (Putative aminoacrylate hydrolase RutD OS=Methylorubrum extorquens (strain DSM 6343 / CIP 106787 / DM4) OX=661410 GN=rutD PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.2e-09
Identity = 39/120 (32.50%), Postives = 57/120 (47.50%), Query Frame = 0


Query: 93  VCAFDNRGMGRSSAPTKKSEYTTKIMAQDAIALLDHLGWEKVHVFGHSMGGMIACKLAAI 152
           V  +D+RG GRS  P +   +    MA+D + LLDHLG     + GH++GG+IA +LA  
Sbjct: 44  VVTYDHRGTGRSPGPLEPG-HDIAAMARDVLDLLDHLGIGTADIVGHALGGLIALQLALT 103

Query: 153 APERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLEE 212
            PERV  + ++N    G+       R+ F      L+   PE          Y   +L E
Sbjct: 104 HPERVGRIVVIN----GWAVMDPATRRCFAARKALLRHAGPEAFVRAQAIFLYPAPWLSE 158

[Close]

BLAST of Carg14010-RA vs. ExPASy TrEMBL
Match: A0A6J1K3F3 (uncharacterized protein LOC111491334 OS=Cucurbita maxima OX=3661 GN=LOC111491334 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.2e-155
Identity = 272/275 (98.91%), Postives = 274/275 (99.64%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIEIN+STTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPNDHGNRNTVGNQIAA DGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ
Sbjct: 61  VPNDHGNRNTVGNQIAAGDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKVH+FGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHIFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. ExPASy TrEMBL
Match: A0A6J1H2I9 (uncharacterized protein LOC111459843 OS=Cucurbita moschata OX=3662 GN=LOC111459843 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.0e-155
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIEIN+STTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWN QIKGLTGTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNLQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. ExPASy TrEMBL
Match: A0A0A0KLW7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139370 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.0e-135
Identity = 238/275 (86.55%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIE+N+S+T AED KIN+AIKIFYRTYGRGSTKVLLIIGLAGTHDSW PQIKGLTGTD
Sbjct: 1   MPFIEVNTSSTAAEDVKINSAIKIFYRTYGRGSTKVLLIIGLAGTHDSWGPQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPND G+RN      AA  GEE +FEDGGGIEVCAFDNRGMGRSS PTKKSEYTTKIMA+
Sbjct: 61  VPNDDGDRN------AAHVGEEPTFEDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEK H+FGHSMGGMIACKL A+APERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHIFGHSMGGMIACKLGAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRF+KAKTPEQRASVDLDTHYSKEYLEE+VGFE RR +LYREYV+GISATGMQ++ 
Sbjct: 181 FDIAVRFMKAKTPEQRASVDLDTHYSKEYLEEYVGFEKRRTLLYREYVEGISATGMQSND 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTH+VT K+IECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHQVTRKDIECLQSAGFLVSVIHGR 269

[Close]

BLAST of Carg14010-RA vs. ExPASy TrEMBL
Match: A0A6J1DV10 (uncharacterized protein LOC111024626 OS=Momordica charantia OX=3673 GN=LOC111024626 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.1e-132
Identity = 233/275 (84.73%), Postives = 251/275 (91.27%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIE+NSST G+E+A IN++I +FYRTYGRGSTKVLLIIGLAGTHDSW PQIKGL GTD
Sbjct: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           VPND  +R+  G + AAD  +E++  DGGGIEVCAFDNRGMGRSS PTKKSEYTTKIMA+
Sbjct: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEK HVFGHSMGGMIACKLAA+APERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFE RRAVLYREYVKGIS+TGMQ++Y
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GF GQV ACW H VT KEIECL+SAG LVSVIHGR
Sbjct: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGR 275

[Close]

BLAST of Carg14010-RA vs. ExPASy TrEMBL
Match: A0A1S3AUJ9 (putative aminoacrylate hydrolase RutD OS=Cucumis melo OX=3656 GN=LOC103482970 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.4e-131
Identity = 231/275 (84.00%), Postives = 251/275 (91.27%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPFIE+N+S+T AED KIN+AIKIFYRT+GRGSTKVLLIIGLAGTHDSW PQIKGLTGTD
Sbjct: 1   MPFIEVNTSSTAAEDVKINSAIKIFYRTFGRGSTKVLLIIGLAGTHDSWGPQIKGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
           + ND  +RN      A+    E +F+DGGGIEVCAFDNRGMGRSS PTKKSEYTT IMA+
Sbjct: 61  ISNDDDDRN------ASHVSAEPTFQDGGGIEVCAFDNRGMGRSSVPTKKSEYTTNIMAK 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEK H+FGHSMGGMIACKLAA+APERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHIFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
           FDIAVRF+KAKTPEQRASVDLDTHYSKEYLEE+VGFE RR +LYREYV+GISATGMQ++ 
Sbjct: 181 FDIAVRFMKAKTPEQRASVDLDTHYSKEYLEEYVGFEKRRTLLYREYVEGISATGMQSND 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GFAGQVNACWTHKVT K+IECLQSAGFLVSVIHGR
Sbjct: 241 GFAGQVNACWTHKVTRKDIECLQSAGFLVSVIHGR 269

[Close]

BLAST of Carg14010-RA vs. TAIR 10
Match: AT5G53050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 3.6e-109
Identity = 191/275 (69.45%), Postives = 214/275 (77.82%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPF E+     G E    N AIKIFYRTYG G  K LLIIGLAGTH+SW PQI GLTGTD
Sbjct: 1   MPFCEVVKEDVGPETTLNNAAIKIFYRTYGHGPIKALLIIGLAGTHESWGPQIMGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
            PND             DD +     D  GIEVCAFDNRGMGRSS PT KSEYTT IMA 
Sbjct: 61  KPNDD------------DDDDGGIVSDDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMAN 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           D+I+LLDHLGW+K H+ GHSMG MIACKLAA+APERV SLA+LNVTGGGF+C PKLDR++
Sbjct: 121 DSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTGGGFECFPKLDRKS 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
             IA+RFLKAKTPEQRA+VDLDTHYSK+YLEE VG  TRRA+LY++YVKGIS TGMQ+ Y
Sbjct: 181 LSIAIRFLKAKTPEQRAAVDLDTHYSKDYLEESVGTNTRRAILYQQYVKGISETGMQSKY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GF GQ+NACW HK+T  EIE ++SAGFLVSVIHGR
Sbjct: 241 GFDGQINACWLHKITKVEIELIRSAGFLVSVIHGR 263

[Close]

BLAST of Carg14010-RA vs. TAIR 10
Match: AT5G53050.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 3.6e-109
Identity = 191/275 (69.45%), Postives = 214/275 (77.82%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPF E+     G E    N AIKIFYRTYG G  K LLIIGLAGTH+SW PQI GLTGTD
Sbjct: 1   MPFCEVVKEDVGPETTLNNAAIKIFYRTYGHGPIKALLIIGLAGTHESWGPQIMGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
            PND             DD +     D  GIEVCAFDNRGMGRSS PT KSEYTT IMA 
Sbjct: 61  KPNDD------------DDDDGGIVSDDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMAN 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           D+I+LLDHLGW+K H+ GHSMG MIACKLAA+APERV SLA+LNVTGGGF+C PKLDR++
Sbjct: 121 DSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTGGGFECFPKLDRKS 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
             IA+RFLKAKTPEQRA+VDLDTHYSK+YLEE VG  TRRA+LY++YVKGIS TGMQ+ Y
Sbjct: 181 LSIAIRFLKAKTPEQRAAVDLDTHYSKDYLEESVGTNTRRAILYQQYVKGISETGMQSKY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GF GQ+NACW HK+T  EIE ++SAGFLVSVIHGR
Sbjct: 241 GFDGQINACWLHKITKVEIELIRSAGFLVSVIHGR 263

[Close]

BLAST of Carg14010-RA vs. TAIR 10
Match: AT5G53050.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 392.1 bits (1006), Expect = 3.6e-109
Identity = 191/275 (69.45%), Postives = 214/275 (77.82%), Query Frame = 0


Query: 1   MPFIEINSSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60
           MPF E+     G E    N AIKIFYRTYG G  K LLIIGLAGTH+SW PQI GLTGTD
Sbjct: 1   MPFCEVVKEDVGPETTLNNAAIKIFYRTYGHGPIKALLIIGLAGTHESWGPQIMGLTGTD 60

Query: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120
            PND             DD +     D  GIEVCAFDNRGMGRSS PT KSEYTT IMA 
Sbjct: 61  KPNDD------------DDDDGGIVSDDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMAN 120

Query: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           D+I+LLDHLGW+K H+ GHSMG MIACKLAA+APERV SLA+LNVTGGGF+C PKLDR++
Sbjct: 121 DSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTGGGFECFPKLDRKS 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240
             IA+RFLKAKTPEQRA+VDLDTHYSK+YLEE VG  TRRA+LY++YVKGIS TGMQ+ Y
Sbjct: 181 LSIAIRFLKAKTPEQRAAVDLDTHYSKDYLEESVGTNTRRAILYQQYVKGISETGMQSKY 240

Query: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGR 276
           GF GQ+NACW HK+T  EIE ++SAGFLVSVIHGR
Sbjct: 241 GFDGQINACWLHKITKVEIELIRSAGFLVSVIHGR 263

[Close]

BLAST of Carg14010-RA vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 6.2e-08
Identity = 29/76 (38.16%), Postives = 40/76 (52.63%), Query Frame = 0


Query: 90  GIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQDAIALLDHLGWEKVHVFGHSMGGMIACKL 149
           G    A D RG G S AP  +  YT   +  D + LLD LG ++V + GH  G ++A  L
Sbjct: 51  GYRAIAPDLRGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWL 110

Query: 150 AAIAPERVKSLAMLNV 166
             I P+RV +L   +V
Sbjct: 111 CMIRPDRVNALVNTSV 126

The following BLAST results are available for this feature:

BLAST of Carg14010-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035755.1	1.3e-156	100.00	rutD [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6605790.1	2.8e-156	99.64	hypothetical protein SDJN03_03107, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023532415.1	8.3e-156	99.27	uncharacterized protein LOC111794595 [Cucurbita pepo subsp. pepo]	[more]
XP_022995986.1	2.4e-155	98.91	uncharacterized protein LOC111491334 [Cucurbita maxima]	[more]
XP_022958692.1	4.1e-155	99.27	uncharacterized protein LOC111459843 [Cucurbita moschata]	[more]

BLAST of Carg14010-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8IAD8	9.0e-12	30.41	Putative aminoacrylate hydrolase RutD OS=Azorhizobium caulinodans (strain ATCC 4...	[more]
B7KWT4	4.9e-10	33.33	Putative aminoacrylate hydrolase RutD OS=Methylorubrum extorquens (strain CM4 / ...	[more]
B1ZB18	6.4e-10	33.33	Putative aminoacrylate hydrolase RutD OS=Methylorubrum populi (strain ATCC BAA-7...	[more]
P34913	4.2e-09	38.67	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]
C7CM33	4.2e-09	32.50	Putative aminoacrylate hydrolase RutD OS=Methylorubrum extorquens (strain DSM 63...	[more]

BLAST of Carg14010-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K3F3	1.2e-155	98.91	uncharacterized protein LOC111491334 OS=Cucurbita maxima OX=3661 GN=LOC111491334...	[more]
A0A6J1H2I9	2.0e-155	99.27	uncharacterized protein LOC111459843 OS=Cucurbita moschata OX=3662 GN=LOC1114598...	[more]
A0A0A0KLW7	3.0e-135	86.55	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139...	[more]
A0A6J1DV10	1.1e-132	84.73	uncharacterized protein LOC111024626 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A1S3AUJ9	3.4e-131	84.00	putative aminoacrylate hydrolase RutD OS=Cucumis melo OX=3656 GN=LOC103482970 PE...	[more]

BLAST of Carg14010-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53050.1	3.6e-109	69.45	alpha/beta-Hydrolases superfamily protein	[more]
AT5G53050.2	3.6e-109	69.45	alpha/beta-Hydrolases superfamily protein	[more]
AT5G53050.3	3.6e-109	69.45	alpha/beta-Hydrolases superfamily protein	[more]
AT4G02340.1	6.2e-08	38.16	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 90..105 score: 35.99 coord: 136..149 score: 40.89 coord: 150..163 score: 35.71
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 78..165 e-value: 6.4E-21 score: 75.1
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 13..275 e-value: 1.9E-38 score: 134.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 22..221
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 11..275
None	No IPR available	PANTHER	PTHR43329:SF1	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 11..275

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg14010	Carg14010	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14010-RA:9	Carg14010-RA:9	exon
Carg14010-RA:8	Carg14010-RA:8	exon
Carg14010-RA:7	Carg14010-RA:7	exon
Carg14010-RA:6	Carg14010-RA:6	exon
Carg14010-RA:5	Carg14010-RA:5	exon
Carg14010-RA:4	Carg14010-RA:4	exon
Carg14010-RA:3	Carg14010-RA:3	exon
Carg14010-RA:2	Carg14010-RA:2	exon
Carg14010-RA:1	Carg14010-RA:1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14010-RA:three_prime_utr	Carg14010-RA:three_prime_utr_3	three_prime_UTR
Carg14010-RA:three_prime_utr	Carg14010-RA:three_prime_utr_2	three_prime_UTR
Carg14010-RA:three_prime_utr	Carg14010-RA:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14010-RA:cds	Carg14010-RA:cds_7	CDS
Carg14010-RA:cds	Carg14010-RA:cds_6	CDS
Carg14010-RA:cds	Carg14010-RA:cds_5	CDS
Carg14010-RA:cds	Carg14010-RA:cds_4	CDS
Carg14010-RA:cds	Carg14010-RA:cds_3	CDS
Carg14010-RA:cds	Carg14010-RA:cds_2	CDS
Carg14010-RA:cds	Carg14010-RA:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14010-RA:five_prime_utr	Carg14010-RA:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg14010-RA	Carg14010-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016787	hydrolase activity