Carg14006-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14006-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pentatricopeptide repeat-containing protein mitochondrial
Location	Carg_Chr02: 6624108 .. 6624635 (+)
Sequence length	324
RNA-Seq Expression	Carg14006-RA
Synteny	Carg14006-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACGAAGAAAGGGAAAAATGTAAGTTAAATTGAAAATTAGAAATTAGAGGATGATTAGCAGCAGCAGATTAATTTTGTCATGTAGATAAGATCATGAAGCTTTGAATTGGTCTTCTCAATCTAATTTTAGGAAGTTTAAATTGAAATTGAAACGAAGAATTTGAAAGGCTCTTGACGATCATTCTCCTCTGTCTTCGCTGATTTTCTCTCCGTGTTTTTACAGACCATCCAAGGCAGGTTACAACAAATCCGCCATAGCCATGGAGAATCAGAGCAGCGCCTGTCTGTCCTACGAGAAGCTTAACGGCGTCGCCACCTGGCTCGGCACCAGCGTCGCCTCCGCCTTCTTCGCCTCTCTCGAGCGCTGTTCCTGCATCAATCTCTCAACCTCCGACGACCATGAAGATCCCGATGAGGCTCACGATCGCCCTCTCATGTACTGCACTTCCACTTCTTCCTCCGTCATTCGCAGCTTCGAACCGCTTCCTCCGCCGGTCAATGACGTTGCCAATCTCCCCGTCTGA

mRNA sequence

ATGAAACGAAGAAAGGGAAAAATACCATCCAAGGCAGGTTACAACAAATCCGCCATAGCCATGGAGAATCAGAGCAGCGCCTGTCTGTCCTACGAGAAGCTTAACGGCGTCGCCACCTGGCTCGGCACCAGCGTCGCCTCCGCCTTCTTCGCCTCTCTCGAGCGCTGTTCCTGCATCAATCTCTCAACCTCCGACGACCATGAAGATCCCGATGAGGCTCACGATCGCCCTCTCATGTACTGCACTTCCACTTCTTCCTCCGTCATTCGCAGCTTCGAACCGCTTCCTCCGCCGGTCAATGACGTTGCCAATCTCCCCGTCTGA

Coding sequence (CDS)

ATGAAACGAAGAAAGGGAAAAATACCATCCAAGGCAGGTTACAACAAATCCGCCATAGCCATGGAGAATCAGAGCAGCGCCTGTCTGTCCTACGAGAAGCTTAACGGCGTCGCCACCTGGCTCGGCACCAGCGTCGCCTCCGCCTTCTTCGCCTCTCTCGAGCGCTGTTCCTGCATCAATCTCTCAACCTCCGACGACCATGAAGATCCCGATGAGGCTCACGATCGCCCTCTCATGTACTGCACTTCCACTTCTTCCTCCGTCATTCGCAGCTTCGAACCGCTTCCTCCGCCGGTCAATGACGTTGCCAATCTCCCCGTCTGA

Protein sequence

MKRRKGKIPSKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV

Homology

[Close]

BLAST of Carg14006-RA vs. NCBI nr
Match: KAG7035759.1 (hypothetical protein SDJN02_02557, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 214.9 bits (546), Expect = 3.3e-52
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = 0


Query: 1   MKRRKGKIPSKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCIN 60
           MKRRKGKIPSKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCIN
Sbjct: 1   MKRRKGKIPSKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCIN 60

Query: 61  LSTSDDHEDPDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV 108
           LSTSDDHEDPDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 61  LSTSDDHEDPDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV 107

[Close]

BLAST of Carg14006-RA vs. NCBI nr
Match: KAG6605795.1 (hypothetical protein SDJN03_03112, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 201.1 bits (510), Expect = 5.0e-48
Identity = 99/99 (100.00%), Postives = 99/99 (100.00%), Query Frame = 0


Query: 9   PSKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHE 68
           PSKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHE
Sbjct: 39  PSKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHE 98

Query: 69  DPDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV 108
           DPDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 99  DPDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV 137

[Close]

BLAST of Carg14006-RA vs. NCBI nr
Match: KAG7016815.1 (hypothetical protein SDJN02_21926, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 182.2 bits (461), Expect = 2.4e-42
Identity = 91/98 (92.86%), Postives = 95/98 (96.94%), Query Frame = 0


Query: 10  SKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHED 69
           SKAG N+SAIAMENQS+ CLSYEKLNGVATW+GTSVASAFFASLE CSCINLSTSDDHED
Sbjct: 4   SKAGKNESAIAMENQST-CLSYEKLNGVATWVGTSVASAFFASLESCSCINLSTSDDHED 63

Query: 70  PDEAHDRPLMYCTSTSSSVIRSFEPLPPPVNDVANLPV 108
           PDEAHDRPLMYCTSTSSS+IRSFEPLPPPVNDVANLPV
Sbjct: 64  PDEAHDRPLMYCTSTSSSIIRSFEPLPPPVNDVANLPV 100

[Close]

BLAST of Carg14006-RA vs. NCBI nr
Match: XP_022958087.1 (uncharacterized protein LOC111459420 [Cucurbita moschata] >XP_022958088.1 uncharacterized protein LOC111459420 [Cucurbita moschata] >XP_023521613.1 uncharacterized protein LOC111785453 [Cucurbita pepo subsp. pepo] >XP_023521614.1 uncharacterized protein LOC111785453 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 179.1 bits (453), Expect = 2.0e-41
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 21  MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 80
           MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY
Sbjct: 1   MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 60

Query: 81  CTSTSSSVIRSFEPLPPPVNDVANLPV 108
           CTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 61  CTSTSSSVIRSFEPLPPPVNDVANLPV 87

[Close]

BLAST of Carg14006-RA vs. NCBI nr
Match: XP_022995453.1 (uncharacterized protein LOC111490985 [Cucurbita maxima] >XP_022995454.1 uncharacterized protein LOC111490985 [Cucurbita maxima] >XP_022995455.1 uncharacterized protein LOC111490985 [Cucurbita maxima])

HSP 1 Score: 175.3 bits (443), Expect = 2.9e-40
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 21  MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 80
           MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHED DEAHDRPLMY
Sbjct: 1   MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDLDEAHDRPLMY 60

Query: 81  CTSTSSSVIRSFEPLPPPVNDVANLPV 108
           CTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 61  CTSTSSSVIRSFEPLPPPVNDVANLPV 87

[Close]

BLAST of Carg14006-RA vs. ExPASy TrEMBL
Match: A0A6J1H0Y4 (uncharacterized protein LOC111459420 OS=Cucurbita moschata OX=3662 GN=LOC111459420 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 9.8e-42
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 21  MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 80
           MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY
Sbjct: 1   MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 60

Query: 81  CTSTSSSVIRSFEPLPPPVNDVANLPV 108
           CTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 61  CTSTSSSVIRSFEPLPPPVNDVANLPV 87

[Close]

BLAST of Carg14006-RA vs. ExPASy TrEMBL
Match: A0A6J1K418 (uncharacterized protein LOC111490985 OS=Cucurbita maxima OX=3661 GN=LOC111490985 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.4e-40
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 21  MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 80
           MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHED DEAHDRPLMY
Sbjct: 1   MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDLDEAHDRPLMY 60

Query: 81  CTSTSSSVIRSFEPLPPPVNDVANLPV 108
           CTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 61  CTSTSSSVIRSFEPLPPPVNDVANLPV 87

[Close]

BLAST of Carg14006-RA vs. ExPASy TrEMBL
Match: A0A1S3AU63 (uncharacterized protein LOC103482984 OS=Cucumis melo OX=3656 GN=LOC103482984 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 4.5e-39
Identity = 91/106 (85.85%), Postives = 95/106 (89.62%), Query Frame = 0


Query: 7   KIPSKAG----YNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLS 66
           KI SKAG      KS I MENQS+ CLSYEKLNG+ATW+GTSVASAFFASLERCSCINLS
Sbjct: 16  KIRSKAGKKKKKKKSVIPMENQST-CLSYEKLNGMATWVGTSVASAFFASLERCSCINLS 75

Query: 67  TSDDHEDPDEAHDRPLMYCTSTSSS-VIRSFEPLPPPVNDVANLPV 108
           TSDDHEDP+EAHDRPLMYCTSTSSS VIRSFEPLPPPVNDVANLPV
Sbjct: 76  TSDDHEDPEEAHDRPLMYCTSTSSSVVIRSFEPLPPPVNDVANLPV 120

[Close]

BLAST of Carg14006-RA vs. ExPASy TrEMBL
Match: A0A6J1E5Z9 (uncharacterized protein LOC111430272 OS=Cucurbita moschata OX=3662 GN=LOC111430272 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.7e-38
Identity = 83/87 (95.40%), Postives = 85/87 (97.70%), Query Frame = 0


Query: 21  MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 80
           MENQS+ CLSYEKLNGVATW+GTSVASAFFASLE CSCINLSTSDDHEDPDEAHDRPLMY
Sbjct: 1   MENQST-CLSYEKLNGVATWVGTSVASAFFASLESCSCINLSTSDDHEDPDEAHDRPLMY 60

Query: 81  CTSTSSSVIRSFEPLPPPVNDVANLPV 108
           CTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 61  CTSTSSSVIRSFEPLPPPVNDVANLPV 86

[Close]

BLAST of Carg14006-RA vs. ExPASy TrEMBL
Match: A0A0A0KLU3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139175 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.3e-38
Identity = 82/87 (94.25%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 21  MENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHEDPDEAHDRPLMY 80
           MENQS+ CLSYEKLNG+ATW+GTSVASAFFASLERCSCINLSTSDDHEDP+EAHDRPLMY
Sbjct: 1   MENQST-CLSYEKLNGMATWVGTSVASAFFASLERCSCINLSTSDDHEDPEEAHDRPLMY 60

Query: 81  CTSTSSSVIRSFEPLPPPVNDVANLPV 108
           CTSTSSSVIRSFEPLPPPVNDVANLPV
Sbjct: 61  CTSTSSSVIRSFEPLPPPVNDVANLPV 86

[Close]

BLAST of Carg14006-RA vs. TAIR 10
Match: AT4G10810.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G24026.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 73.6 bits (179), Expect = 1.1e-13
Identity = 37/69 (53.62%), Postives = 51/69 (73.91%), Query Frame = 0


Query: 10 SKAGYNKSAIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTSDDHED 69
          + +  N +A    N S+A  S EKL+  A+W+ T+V SAFFASLERCSC+NLSTSDD ++
Sbjct: 3  NSSSINSTASTASNLSTA--SLEKLDQAASWVSTTVISAFFASLERCSCVNLSTSDDDDE 62

Query: 70 PDEAHDRPL 79
           +E+H+RPL
Sbjct: 63 GEESHNRPL 69

[Close]

BLAST of Carg14006-RA vs. TAIR 10
Match: AT4G24026.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G10810.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 66.2 bits (160), Expect = 1.8e-11
Identity = 32/63 (50.79%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 18 AIAMENQSSACLSYEKLNGVATWLGTSVASAFFASLERCSCINLSTS--DDHEDPDEAHD 77
          +I++ +  S   S EK++  A+W+  +V SAFF SLERC+C+NLSTS  DD +D DE+H 
Sbjct: 4  SISITSNVSTTTSMEKIDHAASWISATVISAFFTSLERCACVNLSTSHDDDDDDDDESHH 63

Query: 78 RPL 79
          RPL
Sbjct: 64 RPL 66

The following BLAST results are available for this feature:

BLAST of Carg14006-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035759.1	3.3e-52	100.00	hypothetical protein SDJN02_02557, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6605795.1	5.0e-48	100.00	hypothetical protein SDJN03_03112, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7016815.1	2.4e-42	92.86	hypothetical protein SDJN02_21926, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022958087.1	2.0e-41	100.00	uncharacterized protein LOC111459420 [Cucurbita moschata] >XP_022958088.1 unchar...	[more]
XP_022995453.1	2.9e-40	98.85	uncharacterized protein LOC111490985 [Cucurbita maxima] >XP_022995454.1 uncharac...	[more]

BLAST of Carg14006-RA vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg14006-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H0Y4	9.8e-42	100.00	uncharacterized protein LOC111459420 OS=Cucurbita moschata OX=3662 GN=LOC1114594...	[more]
A0A6J1K418	1.4e-40	98.85	uncharacterized protein LOC111490985 OS=Cucurbita maxima OX=3661 GN=LOC111490985...	[more]
A0A1S3AU63	4.5e-39	85.85	uncharacterized protein LOC103482984 OS=Cucumis melo OX=3656 GN=LOC103482984 PE=...	[more]
A0A6J1E5Z9	1.7e-38	95.40	uncharacterized protein LOC111430272 OS=Cucurbita moschata OX=3662 GN=LOC1114302...	[more]
A0A0A0KLU3	2.3e-38	94.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139175 PE=4 SV=1	[more]

BLAST of Carg14006-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10810.1	1.1e-13	53.62	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT4G24026.1	1.8e-11	50.79	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34061:SF19	OS10G0502100 PROTEIN	coord: 17..84
None	No IPR available	PANTHER	PTHR34061	PROTEIN, PUTATIVE-RELATED	coord: 17..84

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg14006	Carg14006	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14006-RA:2	Carg14006-RA:2	exon
Carg14006-RA:1	Carg14006-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14006-RA:cds	Carg14006-RA:cds	CDS
Carg14006-RA:cds	Carg14006-RA:cds_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg14006-RA	Carg14006-RA-protein	polypeptide