Carg13743-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13743-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	F-box protein
Location	Carg_Chr03: 4901150 .. 4901947 (+)
Sequence length	576
RNA-Seq Expression	Carg13743-RA
Synteny	Carg13743-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGGGAAAGAAGAGCGGTTGTTTGAAATCGAAAAGCGTCGTCTCTGCTGCTGCCGATAAAGAGCTGGATTTCGGAGTTGTGAGATATGCGCGTGGTTTAGGAAGGAAACGTGTTGTGATTTCTTGCAATCAGGAGGATTCGTCTATCGACTTGACGCCGAAGGCACCGTCGAAGAGGCGCTGCAGCGTCGTCAGTACGACGGTGGACGCTGATTGGTCTCTGCTCGAGGTTCTGCCTCAGGAGATTCTGGTTAGTACAATTACAAAGTTCTTGAGATTGAAATCGATTCGATATTGCACCTTTCAAATCCTTGCATTCGGTCCCTTTTTCGACTATTGTGTCTGAAATGTATTTTTCCCCCTTTCCCCTCTCCATTAGCTTAGGATACTCTGCGGTGTGGACCATGATGACTTGAAACAGCTAGTCCGCGTTTCGAAAACAATTAGCGAAGCGGTAAGCATCACTCGCATCATTCTTCACATTATTTGTTTCCTTCCATTTGTTTCATTGATTCTCATTGATTAAGGGTCTCAATTGCATTTACAGACTTTAATTGCCAAAGAATGGCATTTCGCTTATAGAACCCCCTCAAAGATCCGTGCATTTCGAACTTCCATTGAACTTGACGACAATTTCATCGATCTCGACGAGATTGAAGCCCCAGATGCTCCTGTCACAAGACGATTTAGGTCTCCATTAAGCAGAAAAAAGCTCGCGGACATTTCCGTGGCCTTGTTTGCCGAAGAAGAGCAATGGCCAAGGAAAAAGTACGCCATTGAGACAGAGATGTGA

mRNA sequence

ATGGCGTTGGGAAAGAAGAGCGGTTGTTTGAAATCGAAAAGCGTCGTCTCTGCTGCTGCCGATAAAGAGCTGGATTTCGGAGTTGTGAGATATGCGCGTGGTTTAGGAAGGAAACGTGTTGTGATTTCTTGCAATCAGGAGGATTCGTCTATCGACTTGACGCCGAAGGCACCGTCGAAGAGGCGCTGCAGCGTCGTCAGTACGACGGTGGACGCTGATTGGTCTCTGCTCGAGGTTCTGCCTCAGGAGATTCTGCTTAGGATACTCTGCGGTGTGGACCATGATGACTTGAAACAGCTAGTCCGCGTTTCGAAAACAATTAGCGAAGCGACTTTAATTGCCAAAGAATGGCATTTCGCTTATAGAACCCCCTCAAAGATCCGTGCATTTCGAACTTCCATTGAACTTGACGACAATTTCATCGATCTCGACGAGATTGAAGCCCCAGATGCTCCTGTCACAAGACGATTTAGGTCTCCATTAAGCAGAAAAAAGCTCGCGGACATTTCCGTGGCCTTGTTTGCCGAAGAAGAGCAATGGCCAAGGAAAAAGTACGCCATTGAGACAGAGATGTGA

Coding sequence (CDS)

ATGGCGTTGGGAAAGAAGAGCGGTTGTTTGAAATCGAAAAGCGTCGTCTCTGCTGCTGCCGATAAAGAGCTGGATTTCGGAGTTGTGAGATATGCGCGTGGTTTAGGAAGGAAACGTGTTGTGATTTCTTGCAATCAGGAGGATTCGTCTATCGACTTGACGCCGAAGGCACCGTCGAAGAGGCGCTGCAGCGTCGTCAGTACGACGGTGGACGCTGATTGGTCTCTGCTCGAGGTTCTGCCTCAGGAGATTCTGCTTAGGATACTCTGCGGTGTGGACCATGATGACTTGAAACAGCTAGTCCGCGTTTCGAAAACAATTAGCGAAGCGACTTTAATTGCCAAAGAATGGCATTTCGCTTATAGAACCCCCTCAAAGATCCGTGCATTTCGAACTTCCATTGAACTTGACGACAATTTCATCGATCTCGACGAGATTGAAGCCCCAGATGCTCCTGTCACAAGACGATTTAGGTCTCCATTAAGCAGAAAAAAGCTCGCGGACATTTCCGTGGCCTTGTTTGCCGAAGAAGAGCAATGGCCAAGGAAAAAGTACGCCATTGAGACAGAGATGTGA

Protein sequence

MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSKRRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFAYRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQWPRKKYAIETEM

Homology

[Close]

BLAST of Carg13743-RA vs. NCBI nr
Match: XP_022950447.1 (F-box protein SKIP27-like [Cucurbita moschata] >KAG6603722.1 F-box protein SKIP27, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033902.1 F-box protein SKIP27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 376.3 bits (965), Expect = 1.5e-100
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK
Sbjct: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA
Sbjct: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW 180
           YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW
Sbjct: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW 180

Query: 181 PRKKYAIETEM 192
           PRKKYAIETEM
Sbjct: 181 PRKKYAIETEM 191

[Close]

BLAST of Carg13743-RA vs. NCBI nr
Match: XP_023544727.1 (F-box protein SKIP27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 373.6 bits (958), Expect = 1.0e-99
Identity = 189/191 (98.95%), Postives = 190/191 (99.48%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK
Sbjct: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRCSVV TTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA
Sbjct: 61  RRCSVVGTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW 180
           YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAP+TRRFRSPLSRKKLADISVALFAEEEQW
Sbjct: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPLTRRFRSPLSRKKLADISVALFAEEEQW 180

Query: 181 PRKKYAIETEM 192
           PRKKYAIETEM
Sbjct: 181 PRKKYAIETEM 191

[Close]

BLAST of Carg13743-RA vs. NCBI nr
Match: XP_022977983.1 (F-box protein SKIP27-like [Cucurbita maxima])

HSP 1 Score: 362.8 bits (930), Expect = 1.8e-96
Identity = 185/191 (96.86%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKKSGCLKSKSVVS AADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPK PSK
Sbjct: 1   MALGKKSGCLKSKSVVS-AADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKTPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRCSVVSTTVDA WSLLEVLPQEIL+RILCG+DHDDLKQLVRVSKTISEATLIAKEWHFA
Sbjct: 61  RRCSVVSTTVDAGWSLLEVLPQEILIRILCGLDHDDLKQLVRVSKTISEATLIAKEWHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW 180
           YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAP+TRRFRSPLSRKKLADISVALFAEEEQW
Sbjct: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPLTRRFRSPLSRKKLADISVALFAEEEQW 180

Query: 181 PRKKYAIETEM 192
           PRKKYAIETEM
Sbjct: 181 PRKKYAIETEM 190

[Close]

BLAST of Carg13743-RA vs. NCBI nr
Match: XP_008439907.1 (PREDICTED: F-box protein At4g05010-like [Cucumis melo] >KAA0052721.1 F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 298.5 bits (763), Expect = 4.1e-77
Identity = 157/192 (81.77%), Postives = 175/192 (91.15%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKK+GCLKSKSVVS AADK LDFGVVRY RGLGRKRVVISCN+EDSSIDLTPKAPSK
Sbjct: 1   MALGKKTGCLKSKSVVS-AADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRC+VVST V+AD SLLE LPQEIL+R+LCGV+HDDLKQL+RVSKTISEATLIAK+ HFA
Sbjct: 61  RRCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEE-Q 180
           Y TPSK+RAFRT+IEL+D+  D DEIEAP+A  TRRFRSPL+RKKLA+ISVALFAEEE +
Sbjct: 121 YSTPSKVRAFRTAIELNDS-SDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDE 180

Query: 181 WPRKKYAIETEM 192
           WP+KK  I TEM
Sbjct: 181 WPKKKLPIWTEM 190

[Close]

BLAST of Carg13743-RA vs. NCBI nr
Match: TYK13104.1 (F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 298.5 bits (763), Expect = 4.1e-77
Identity = 157/192 (81.77%), Postives = 174/192 (90.62%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKK+GCLKSKSVVS AADK LDFGVVRY RGLGRKRVVISCN+EDSSIDLTPKAPSK
Sbjct: 1   MALGKKTGCLKSKSVVS-AADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           R C+VVST V+AD SLLE LPQEIL+R+LCGV+HDDLKQL+RVSKTISEATLIAK+ HFA
Sbjct: 61  RHCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEE-Q 180
           Y TPSK+RAFRT+IELDD+  D DEIEAP+A  TRRFRSPL+RKKLA+ISVALFAEEE +
Sbjct: 121 YSTPSKVRAFRTAIELDDS-SDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDE 180

Query: 181 WPRKKYAIETEM 192
           WP+KK  I TEM
Sbjct: 181 WPKKKLPIWTEM 190

[Close]

BLAST of Carg13743-RA vs. ExPASy Swiss-Prot
Match: O65416 (F-box protein SKIP27 OS=Arabidopsis thaliana OX=3702 GN=SKIP27 PE=1 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 7.3e-29
Identity = 80/192 (41.67%), Postives = 119/192 (61.98%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADK-ELDFGVVRYARGLGRKRVVI-SCNQEDSS-----IDL 60
           MAL K+   + S +      ++ EL  G VR+ RGLGRKR++I SC +E  S     I +
Sbjct: 34  MALSKRGFVMTSNARFHGEEEELELGLGSVRFTRGLGRKRILISSCVRESLSRSAVEIPV 93

Query: 61  TPKAP--------SKRRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSK 120
             ++P         + R ++VS++  ++ S LE LPQ++L+R++CGVDH+DLK L  VSK
Sbjct: 94  VSESPPVKSSLKRQRSRITIVSSS-SSEKSRLECLPQDLLIRVICGVDHEDLKSLKLVSK 153

Query: 121 TISEATLIAKEWHFAYRTPSKIRAFRTSIELD---DNFIDLDEIEAPDAPVTRRFRSPLS 175
           +I EA+L+AK  HFAY TP K RAFR SI+L+   D+    D+IE P+AP   R+     
Sbjct: 154 SIREASLVAKTLHFAYTTPKKTRAFRNSIDLEEVSDSRHQEDDIEPPNAPRHYRWTKAKR 213

[Close]

BLAST of Carg13743-RA vs. ExPASy Swiss-Prot
Match: Q8GX77 (F-box protein At1g61340 OS=Arabidopsis thaliana OX=3702 GN=At1g61340 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.9e-24
Identity = 83/191 (43.46%), Postives = 113/191 (59.16%), Query Frame = 0


Query: 1   MALGK------KSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQE-DSSIDL 60
           MALGK      K    + + V +      L+F  V+Y RG GRKR++IS   E + SI  
Sbjct: 1   MALGKKRIVTQKPNLRQRRDVDNGGLGLGLEF--VQYKRGFGRKRILISSGDEMEDSIFT 60

Query: 61  TPKAPSKRRCSVVSTTVDADWSL-LEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATL 120
           +P    K+ C   +T+V    S  LE LP +IL+RI+CGV+H+DLKQL  VSKTI EAT+
Sbjct: 61  SP--VGKKLCDDKTTSVAEGQSRELEDLPLDILVRIICGVEHEDLKQLFHVSKTIREATM 120

Query: 121 IAKEWHFAYRTPSKIRAFR-------TSIELDDNFIDLDEIEAPDAPVTRRFR-SPLSRK 175
           IAK+ HFAY TP K   F           +++D   D +EIEAP AP+ +R+R S ++R 
Sbjct: 121 IAKQSHFAYSTPRKTSVFHHGRFGWDKPFDVED---DDEEIEAPGAPLQKRYRLSRINRN 180

[Close]

BLAST of Carg13743-RA vs. ExPASy Swiss-Prot
Match: Q9S9T6 (F-box protein At4g05010 OS=Arabidopsis thaliana OX=3702 GN=At4g05010 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 6.6e-22
Identity = 67/157 (42.68%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 22  KELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSKRRCSVVSTTVDADWSLLEVLP 81
           + L  G VR+ RGLGRKR++IS    ++       +P  +R S    T ++  SLLE L 
Sbjct: 17  ERLGLGFVRFTRGLGRKRILISKRAPEND------SPPVKRPS--HETTESCRSLLETLH 76

Query: 82  QEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFAYRTPSKIRAFRTSIELDDNFI 141
           Q+IL+R+LC VDH+DL  L RVSKTI +A + AK+ HF Y TP K   FR +I + D+  
Sbjct: 77  QDILIRVLCHVDHEDLATLKRVSKTIRKAVIEAKKSHFDYSTPKKRLPFRDAILILDSNS 136

Query: 142 DL----DEIEAPDAPVTRRFRSPLSRKKLADISVALF 175
           +     DE+E P+AP+ RRF +      L+ IS+ LF
Sbjct: 137 NSSSQDDEMEPPNAPIRRRFIN--RESDLSKISMVLF 163

[Close]

BLAST of Carg13743-RA vs. ExPASy Swiss-Prot
Match: Q5XF11 (F-box protein At4g35930 OS=Arabidopsis thaliana OX=3702 GN=At4g35930 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.9e-09
Identity = 45/145 (31.03%), Postives = 69/145 (47.59%), Query Frame = 0


Query: 54  TPKAPSKRRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLI 113
           TPK P    C           S LE LP ++L++I+C + HD LK +  VS+ I  AT++
Sbjct: 147 TPKTPQADPC--------VSESQLESLPMDLLVKIVCHLHHDQLKAVFHVSQRIRMATIL 206

Query: 114 AKEWHFAYRTPSKIR----AFRTSIEL--------DDNFIDLDEIEAPDAPVTRRFRSPL 173
           A+++HF Y TP + R       T + +        D N   +     P AP  +    P 
Sbjct: 207 ARQYHFNYTTPDRSRQEMLRVMTPVPINRWPFRRGDGNPTMVSSPHTPKAP--KHAPRPP 266

Query: 174 SRKKLAD---ISVALFAEEEQWPRK 184
           SR KLA+   I+  LF ++  +P +
Sbjct: 267 SRTKLAEMKQITAVLFQDQTPFPSR 281

[Close]

BLAST of Carg13743-RA vs. ExPASy TrEMBL
Match: A0A6J1GEU1 (F-box protein SKIP27-like OS=Cucurbita moschata OX=3662 GN=LOC111453544 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 7.5e-101
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK
Sbjct: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA
Sbjct: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW 180
           YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW
Sbjct: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW 180

Query: 181 PRKKYAIETEM 192
           PRKKYAIETEM
Sbjct: 181 PRKKYAIETEM 191

[Close]

BLAST of Carg13743-RA vs. ExPASy TrEMBL
Match: A0A6J1IRQ4 (F-box protein SKIP27-like OS=Cucurbita maxima OX=3661 GN=LOC111478110 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 8.5e-97
Identity = 185/191 (96.86%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKKSGCLKSKSVVS AADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPK PSK
Sbjct: 1   MALGKKSGCLKSKSVVS-AADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKTPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRCSVVSTTVDA WSLLEVLPQEIL+RILCG+DHDDLKQLVRVSKTISEATLIAKEWHFA
Sbjct: 61  RRCSVVSTTVDAGWSLLEVLPQEILIRILCGLDHDDLKQLVRVSKTISEATLIAKEWHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEEQW 180
           YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAP+TRRFRSPLSRKKLADISVALFAEEEQW
Sbjct: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPLTRRFRSPLSRKKLADISVALFAEEEQW 180

Query: 181 PRKKYAIETEM 192
           PRKKYAIETEM
Sbjct: 181 PRKKYAIETEM 190

[Close]

BLAST of Carg13743-RA vs. ExPASy TrEMBL
Match: A0A5A7UGT0 (F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003340 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.0e-77
Identity = 157/192 (81.77%), Postives = 175/192 (91.15%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKK+GCLKSKSVVS AADK LDFGVVRY RGLGRKRVVISCN+EDSSIDLTPKAPSK
Sbjct: 1   MALGKKTGCLKSKSVVS-AADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRC+VVST V+AD SLLE LPQEIL+R+LCGV+HDDLKQL+RVSKTISEATLIAK+ HFA
Sbjct: 61  RRCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEE-Q 180
           Y TPSK+RAFRT+IEL+D+  D DEIEAP+A  TRRFRSPL+RKKLA+ISVALFAEEE +
Sbjct: 121 YSTPSKVRAFRTAIELNDS-SDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDE 180

Query: 181 WPRKKYAIETEM 192
           WP+KK  I TEM
Sbjct: 181 WPKKKLPIWTEM 190

[Close]

BLAST of Carg13743-RA vs. ExPASy TrEMBL
Match: A0A5D3CPZ7 (F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007130 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.0e-77
Identity = 157/192 (81.77%), Postives = 174/192 (90.62%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKK+GCLKSKSVVS AADK LDFGVVRY RGLGRKRVVISCN+EDSSIDLTPKAPSK
Sbjct: 1   MALGKKTGCLKSKSVVS-AADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           R C+VVST V+AD SLLE LPQEIL+R+LCGV+HDDLKQL+RVSKTISEATLIAK+ HFA
Sbjct: 61  RHCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEE-Q 180
           Y TPSK+RAFRT+IELDD+  D DEIEAP+A  TRRFRSPL+RKKLA+ISVALFAEEE +
Sbjct: 121 YSTPSKVRAFRTAIELDDS-SDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDE 180

Query: 181 WPRKKYAIETEM 192
           WP+KK  I TEM
Sbjct: 181 WPKKKLPIWTEM 190

[Close]

BLAST of Carg13743-RA vs. ExPASy TrEMBL
Match: A0A1S3B0I0 (F-box protein At4g05010-like OS=Cucumis melo OX=3656 GN=LOC103484553 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.0e-77
Identity = 157/192 (81.77%), Postives = 175/192 (91.15%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSK 60
           MALGKK+GCLKSKSVVS AADK LDFGVVRY RGLGRKRVVISCN+EDSSIDLTPKAPSK
Sbjct: 1   MALGKKTGCLKSKSVVS-AADKGLDFGVVRYTRGLGRKRVVISCNEEDSSIDLTPKAPSK 60

Query: 61  RRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFA 120
           RRC+VVST V+AD SLLE LPQEIL+R+LCGV+HDDLKQL+RVSKTISEATLIAK+ HFA
Sbjct: 61  RRCNVVSTAVEADRSLLEALPQEILIRVLCGVEHDDLKQLIRVSKTISEATLIAKDSHFA 120

Query: 121 YRTPSKIRAFRTSIELDDNFIDLDEIEAPDAPVTRRFRSPLSRKKLADISVALFAEEE-Q 180
           Y TPSK+RAFRT+IEL+D+  D DEIEAP+A  TRRFRSPL+RKKLA+ISVALFAEEE +
Sbjct: 121 YSTPSKVRAFRTAIELNDS-SDFDEIEAPNARPTRRFRSPLNRKKLAEISVALFAEEEDE 180

Query: 181 WPRKKYAIETEM 192
           WP+KK  I TEM
Sbjct: 181 WPKKKLPIWTEM 190

[Close]

BLAST of Carg13743-RA vs. TAIR 10
Match: AT4G21510.1 (F-box family protein )

HSP 1 Score: 128.6 bits (322), Expect = 5.2e-30
Identity = 80/192 (41.67%), Postives = 119/192 (61.98%), Query Frame = 0


Query: 1   MALGKKSGCLKSKSVVSAAADK-ELDFGVVRYARGLGRKRVVI-SCNQEDSS-----IDL 60
           MAL K+   + S +      ++ EL  G VR+ RGLGRKR++I SC +E  S     I +
Sbjct: 34  MALSKRGFVMTSNARFHGEEEELELGLGSVRFTRGLGRKRILISSCVRESLSRSAVEIPV 93

Query: 61  TPKAP--------SKRRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSK 120
             ++P         + R ++VS++  ++ S LE LPQ++L+R++CGVDH+DLK L  VSK
Sbjct: 94  VSESPPVKSSLKRQRSRITIVSSS-SSEKSRLECLPQDLLIRVICGVDHEDLKSLKLVSK 153

Query: 121 TISEATLIAKEWHFAYRTPSKIRAFRTSIELD---DNFIDLDEIEAPDAPVTRRFRSPLS 175
           +I EA+L+AK  HFAY TP K RAFR SI+L+   D+    D+IE P+AP   R+     
Sbjct: 154 SIREASLVAKTLHFAYTTPKKTRAFRNSIDLEEVSDSRHQEDDIEPPNAPRHYRWTKAKR 213

[Close]

BLAST of Carg13743-RA vs. TAIR 10
Match: AT1G61340.1 (F-box family protein )

HSP 1 Score: 114.0 bits (284), Expect = 1.3e-25
Identity = 83/191 (43.46%), Postives = 113/191 (59.16%), Query Frame = 0


Query: 1   MALGK------KSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQE-DSSIDL 60
           MALGK      K    + + V +      L+F  V+Y RG GRKR++IS   E + SI  
Sbjct: 1   MALGKKRIVTQKPNLRQRRDVDNGGLGLGLEF--VQYKRGFGRKRILISSGDEMEDSIFT 60

Query: 61  TPKAPSKRRCSVVSTTVDADWSL-LEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATL 120
           +P    K+ C   +T+V    S  LE LP +IL+RI+CGV+H+DLKQL  VSKTI EAT+
Sbjct: 61  SP--VGKKLCDDKTTSVAEGQSRELEDLPLDILVRIICGVEHEDLKQLFHVSKTIREATM 120

Query: 121 IAKEWHFAYRTPSKIRAFR-------TSIELDDNFIDLDEIEAPDAPVTRRFR-SPLSRK 175
           IAK+ HFAY TP K   F           +++D   D +EIEAP AP+ +R+R S ++R 
Sbjct: 121 IAKQSHFAYSTPRKTSVFHHGRFGWDKPFDVED---DDEEIEAPGAPLQKRYRLSRINRN 180

[Close]

BLAST of Carg13743-RA vs. TAIR 10
Match: AT4G05010.1 (F-box family protein )

HSP 1 Score: 105.5 bits (262), Expect = 4.7e-23
Identity = 67/157 (42.68%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 22  KELDFGVVRYARGLGRKRVVISCNQEDSSIDLTPKAPSKRRCSVVSTTVDADWSLLEVLP 81
           + L  G VR+ RGLGRKR++IS    ++       +P  +R S    T ++  SLLE L 
Sbjct: 17  ERLGLGFVRFTRGLGRKRILISKRAPEND------SPPVKRPS--HETTESCRSLLETLH 76

Query: 82  QEILLRILCGVDHDDLKQLVRVSKTISEATLIAKEWHFAYRTPSKIRAFRTSIELDDNFI 141
           Q+IL+R+LC VDH+DL  L RVSKTI +A + AK+ HF Y TP K   FR +I + D+  
Sbjct: 77  QDILIRVLCHVDHEDLATLKRVSKTIRKAVIEAKKSHFDYSTPKKRLPFRDAILILDSNS 136

Query: 142 DL----DEIEAPDAPVTRRFRSPLSRKKLADISVALF 175
           +     DE+E P+AP+ RRF +      L+ IS+ LF
Sbjct: 137 NSSSQDDEMEPPNAPIRRRFIN--RESDLSKISMVLF 163

[Close]

BLAST of Carg13743-RA vs. TAIR 10
Match: AT1G61340.2 (F-box family protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.4e-19
Identity = 84/237 (35.44%), Postives = 113/237 (47.68%), Query Frame = 0


Query: 1   MALGK------KSGCLKSKSVVSAAADKELDFGVVRYARGLGRKRVVISCNQE-DSSIDL 60
           MALGK      K    + + V +      L+F  V+Y RG GRKR++IS   E + SI  
Sbjct: 1   MALGKKRIVTQKPNLRQRRDVDNGGLGLGLEF--VQYKRGFGRKRILISSGDEMEDSIFT 60

Query: 61  TPKAPSKRRCSVVSTTVDADWSL-LEVLPQEILL-------------------------- 120
           +P    K+ C   +T+V    S  LE LP +ILL                          
Sbjct: 61  SP--VGKKLCDDKTTSVAEGQSRELEDLPLDILLRVFVNSASVNQIELKVESFLFSEIQN 120

Query: 121 --------------------RILCGVDHDDLKQLVRVSKTISEATLIAKEWHFAYRTPSK 175
                               RI+CGV+H+DLKQL  VSKTI EAT+IAK+ HFAY TP K
Sbjct: 121 RNGNEKMFNFFHIWKTFVIVRIICGVEHEDLKQLFHVSKTIREATMIAKQSHFAYSTPRK 180

[Close]

BLAST of Carg13743-RA vs. TAIR 10
Match: AT4G35930.1 (F-box family protein )

HSP 1 Score: 62.8 bits (151), Expect = 3.5e-10
Identity = 45/145 (31.03%), Postives = 69/145 (47.59%), Query Frame = 0


Query: 54  TPKAPSKRRCSVVSTTVDADWSLLEVLPQEILLRILCGVDHDDLKQLVRVSKTISEATLI 113
           TPK P    C           S LE LP ++L++I+C + HD LK +  VS+ I  AT++
Sbjct: 147 TPKTPQADPC--------VSESQLESLPMDLLVKIVCHLHHDQLKAVFHVSQRIRMATIL 206

Query: 114 AKEWHFAYRTPSKIR----AFRTSIEL--------DDNFIDLDEIEAPDAPVTRRFRSPL 173
           A+++HF Y TP + R       T + +        D N   +     P AP  +    P 
Sbjct: 207 ARQYHFNYTTPDRSRQEMLRVMTPVPINRWPFRRGDGNPTMVSSPHTPKAP--KHAPRPP 266

Query: 174 SRKKLAD---ISVALFAEEEQWPRK 184
           SR KLA+   I+  LF ++  +P +
Sbjct: 267 SRTKLAEMKQITAVLFQDQTPFPSR 281

The following BLAST results are available for this feature:

BLAST of Carg13743-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950447.1	1.5e-100	100.00	F-box protein SKIP27-like [Cucurbita moschata] >KAG6603722.1 F-box protein SKIP2...	[more]
XP_023544727.1	1.0e-99	98.95	F-box protein SKIP27-like [Cucurbita pepo subsp. pepo]	[more]
XP_022977983.1	1.8e-96	96.86	F-box protein SKIP27-like [Cucurbita maxima]	[more]
XP_008439907.1	4.1e-77	81.77	PREDICTED: F-box protein At4g05010-like [Cucumis melo] >KAA0052721.1 F-box prote...	[more]
TYK13104.1	4.1e-77	81.77	F-box protein [Cucumis melo var. makuwa]	[more]

BLAST of Carg13743-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65416	7.3e-29	41.67	F-box protein SKIP27 OS=Arabidopsis thaliana OX=3702 GN=SKIP27 PE=1 SV=2	[more]
Q8GX77	1.9e-24	43.46	F-box protein At1g61340 OS=Arabidopsis thaliana OX=3702 GN=At1g61340 PE=2 SV=1	[more]
Q9S9T6	6.6e-22	42.68	F-box protein At4g05010 OS=Arabidopsis thaliana OX=3702 GN=At4g05010 PE=2 SV=1	[more]
Q5XF11	4.9e-09	31.03	F-box protein At4g35930 OS=Arabidopsis thaliana OX=3702 GN=At4g35930 PE=2 SV=1	[more]

BLAST of Carg13743-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GEU1	7.5e-101	100.00	F-box protein SKIP27-like OS=Cucurbita moschata OX=3662 GN=LOC111453544 PE=4 SV=...	[more]
A0A6J1IRQ4	8.5e-97	96.86	F-box protein SKIP27-like OS=Cucurbita maxima OX=3661 GN=LOC111478110 PE=4 SV=1	[more]
A0A5A7UGT0	2.0e-77	81.77	F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003340...	[more]
A0A5D3CPZ7	2.0e-77	81.77	F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007130...	[more]
A0A1S3B0I0	2.0e-77	81.77	F-box protein At4g05010-like OS=Cucumis melo OX=3656 GN=LOC103484553 PE=4 SV=1	[more]

BLAST of Carg13743-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21510.1	5.2e-30	41.67	F-box family protein	[more]
AT1G61340.1	1.3e-25	43.46	F-box family protein	[more]
AT4G05010.1	4.7e-23	42.68	F-box family protein	[more]
AT1G61340.2	2.4e-19	35.44	F-box family protein	[more]
AT4G35930.1	3.5e-10	31.03	F-box family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34049	F-BOX PROTEIN SKIP27	coord: 4..178
None	No IPR available	PANTHER	PTHR34049:SF1	F-BOX PROTEIN SKIP27	coord: 4..178
IPR001810	F-box domain	PROSITE	PS50181	FBOX	coord: 74..124 score: 9.100053
IPR036047	F-box-like domain superfamily	SUPERFAMILY	81383	F-box domain	coord: 73..112

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg13743	Carg13743	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg13743-RA:3	Carg13743-RA:3	exon
Carg13743-RA:2	Carg13743-RA:2	exon
Carg13743-RA:1	Carg13743-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg13743-RA:cds	Carg13743-RA:cds	CDS
Carg13743-RA:cds	Carg13743-RA:cds_2	CDS
Carg13743-RA:cds	Carg13743-RA:cds_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg13743-RA	Carg13743-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding