Carg13727-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13727-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontranscription factor bHLH112-like
LocationCarg_Chr03: 4971901 .. 4974018 (-)
Sequence length1149
RNA-Seq ExpressionCarg13727-RA
SyntenyCarg13727-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGGGTTTCCAATATGGCAGAGGATTTTCAGGCTGCTATTTGCGGCGATAATTGGTGGAGCTCTTCCCGGACCATGTTGGTGGCTGTCGGTGGATTGTCGCCGTGTTCTATGGGGATCGGGGACTTAGCTTGGCCGCCGGCCGATGATTTTTCCGACATCAAACCCAAATCCACCGCTAATTATTCCGACAATCAGAACTCCATGTTTTTTCAAGATTATTCCACCTCCGATCATTGGAATCAACCCCATGATTTGCTGTAAGTGACCCACAAACAAAAACAAAAAAAGATTCGATTTTTATTAATCAATTTTGCTTAATTTTTGTTGTTGTGGAAACAGGTTGAAGCGAGGAGATTATTTGTTGGAACAAAATGGGGTGAATTGTATGAACTGTTGTTCTTCCACTAGCCAGGGTTTGTTTCCTCTTGACTCAGCTTTATATGGATACCCTTCCCCTTCAACTAAGCTGCTCCATGGCTTATACAATAATGATGATGATGATGATGATCATCATCATCCTGACCCTCACTCCCTTTTCAACGCCGCCACCACTTCAATTAACACTAATTATCCTTCTAATTACCTTATTAAACCCTCTCTCCCAATCCCGCCGCCCTCTCAGCCTGTTTCACCGGCCTTGCACTTCACAAACAACACGCCCTTTTGGAATGCCTCGCACGCACCTCCTTCCATTGAAATTCCACAGAATAGTAGGCTTGGTTTCAAGGTATGATTTTGATTGGTATGATTGGGTTTTGGGTGTCACTTGATTGATTTTGATTGTTTTTCTTTAGGCTATGAATAATGGTAATTGTGAAGATGGTGAAGAAGCAAGGAAAGGGGATAAAAATGAGCCGCCGGTGTCGAAAAGGCCTCGAATTGAAACTCCTTCGCCATTGCCAACATTTAAGGTGAAGGATCCCAAAAAAAAAAAAAAAAAAGTTTGAAGAATCTTGGGTTATAAAAAGGACAAAAACTAATGGGTTTGCTATCAGGTGCGGAAAGAGAAGCTAGGGGACCGTGTCACTGCCCTTCAACAACTGGTTTCACCCTTTGGAAAGGTGAGTTTTTGGATTTCATCCCAAAAAAAATATATATCCGAGGTGAACGAAACATTCTTTATGAGTTATAAGTATAAGGGTGTGGAAATCTTTTCTTTGTAGTTTTAAAATTGTGAGGTTGACAACTATATATAACGAGTCAAAACGAACAATATTTGTTAGTGGTGGGTTGGTTATGTGCCAACGAGAACCCTAGGCCTTAAAGGGTGGATTGTGAGATCCCACTTCGGTTGAAAAGGGAAACAAAACATTCCTTATAACGGTGTGTAAACCTCTCCCTAGTAGTTTTAAAATCATGAGGCTGACGATGATATATAATGAGACAAACCGAACAGTATTTACTAGCAGTGGGTTTGAGCGGTAATAAATGGTATCAGAGCTAGACACTGAACGATGTGCTAGTAAGAACGTTGGGCCCCAAGAGAGGTGGATTATGAGATCCCACATCAGTTGGAGAAGAAAATGAAACATTCCTTATAAGAGTGTGGAAGTCTATACTTAGTAGACACGTTTTAAAATCGTGAGGCTGACGATAATTCTCGTCTCTTTTTCCTCCAGACTGATACAGCCTCAGTTCTTCATGAAGCCATTGAGTACATCAAGTTCCTCCACGATCAAGTCAGTGTACGTTCCATTATTTACATAAATTTCATCTTTAATTTCCTTTTAAAACCCAAATTAGTTCATTCATTACTTGAAATTTTCAGCTGTTAAGCACTCCATATATGAAGAACAACAACGGAATAACACCCCTTCAACAACAACAGGTAAAAAAAAATATGAACTTTCTTCAAATTACCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTATAAACTTTACAGTTGCTTGAATTCCAGGTATCAGAAAAAGCAGACCCAAAACAAGATCTGAAAAGCCGAGGCCTTTGTTTGGTGCCGATCTCAAGCACATTCCCAGTGACAAATGCAACAACGACGGATTTCTGGTCACCGACGTTCGGCGGAACATTCCGGTGAAGATATATATATATATATATGAATATAAGATTGAATTTGGTGTAATAATGAAGGG

mRNA sequence

TTGGGGTTTCCAATATGGCAGAGGATTTTCAGGCTGCTATTTGCGGCGATAATTGGTGGAGCTCTTCCCGGACCATGTTGGTGGCTGTCGGTGGATTGTCGCCGTGTTCTATGGGGATCGGGGACTTAGCTTGGCCGCCGGCCGATGATTTTTCCGACATCAAACCCAAATCCACCGCTAATTATTCCGACAATCAGAACTCCATGTTTTTTCAAGATTATTCCACCTCCGATCATTGGAATCAACCCCATGATTTGCTGTTGAAGCGAGGAGATTATTTGTTGGAACAAAATGGGGTGAATTGTATGAACTGTTGTTCTTCCACTAGCCAGGGTTTGTTTCCTCTTGACTCAGCTTTATATGGATACCCTTCCCCTTCAACTAAGCTGCTCCATGGCTTATACAATAATGATGATGATGATGATGATCATCATCATCCTGACCCTCACTCCCTTTTCAACGCCGCCACCACTTCAATTAACACTAATTATCCTTCTAATTACCTTATTAAACCCTCTCTCCCAATCCCGCCGCCCTCTCAGCCTGTTTCACCGGCCTTGCACTTCACAAACAACACGCCCTTTTGGAATGCCTCGCACGCACCTCCTTCCATTGAAATTCCACAGAATAGTAGGCTTGGTTTCAAGGCTATGAATAATGGTAATTGTGAAGATGGTGAAGAAGCAAGGAAAGGGGATAAAAATGAGCCGCCGGTGTCGAAAAGGCCTCGAATTGAAACTCCTTCGCCATTGCCAACATTTAAGGTGCGGAAAGAGAAGCTAGGGGACCGTGTCACTGCCCTTCAACAACTGGTTTCACCCTTTGGAAAGACTGATACAGCCTCAGTTCTTCATGAAGCCATTGAGTACATCAAGTTCCTCCACGATCAAGTCAGTCTGTTAAGCACTCCATATATGAAGAACAACAACGGAATAACACCCCTTCAACAACAACAGGTATCAGAAAAAGCAGACCCAAAACAAGATCTGAAAAGCCGAGGCCTTTGTTTGGTGCCGATCTCAAGCACATTCCCAGTGACAAATGCAACAACGACGGATTTCTGGTCACCGACGTTCGGCGGAACATTCCGGTGAAGATATATATATATATATATGAATATAAGATTGAATTTGGTGTAATAATGAAGGG

Coding sequence (CDS)

ATGGCAGAGGATTTTCAGGCTGCTATTTGCGGCGATAATTGGTGGAGCTCTTCCCGGACCATGTTGGTGGCTGTCGGTGGATTGTCGCCGTGTTCTATGGGGATCGGGGACTTAGCTTGGCCGCCGGCCGATGATTTTTCCGACATCAAACCCAAATCCACCGCTAATTATTCCGACAATCAGAACTCCATGTTTTTTCAAGATTATTCCACCTCCGATCATTGGAATCAACCCCATGATTTGCTGTTGAAGCGAGGAGATTATTTGTTGGAACAAAATGGGGTGAATTGTATGAACTGTTGTTCTTCCACTAGCCAGGGTTTGTTTCCTCTTGACTCAGCTTTATATGGATACCCTTCCCCTTCAACTAAGCTGCTCCATGGCTTATACAATAATGATGATGATGATGATGATCATCATCATCCTGACCCTCACTCCCTTTTCAACGCCGCCACCACTTCAATTAACACTAATTATCCTTCTAATTACCTTATTAAACCCTCTCTCCCAATCCCGCCGCCCTCTCAGCCTGTTTCACCGGCCTTGCACTTCACAAACAACACGCCCTTTTGGAATGCCTCGCACGCACCTCCTTCCATTGAAATTCCACAGAATAGTAGGCTTGGTTTCAAGGCTATGAATAATGGTAATTGTGAAGATGGTGAAGAAGCAAGGAAAGGGGATAAAAATGAGCCGCCGGTGTCGAAAAGGCCTCGAATTGAAACTCCTTCGCCATTGCCAACATTTAAGGTGCGGAAAGAGAAGCTAGGGGACCGTGTCACTGCCCTTCAACAACTGGTTTCACCCTTTGGAAAGACTGATACAGCCTCAGTTCTTCATGAAGCCATTGAGTACATCAAGTTCCTCCACGATCAAGTCAGTCTGTTAAGCACTCCATATATGAAGAACAACAACGGAATAACACCCCTTCAACAACAACAGGTATCAGAAAAAGCAGACCCAAAACAAGATCTGAAAAGCCGAGGCCTTTGTTTGGTGCCGATCTCAAGCACATTCCCAGTGACAAATGCAACAACGACGGATTTCTGGTCACCGACGTTCGGCGGAACATTCCGGTGA

Protein sequence

MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPSPSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSPALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR
Homology
BLAST of Carg13727-RA vs. NCBI nr
Match: KAG7033917.1 (Transcription factor bHLH [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 745.3 bits (1923), Expect = 2.4e-211
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
           QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS
Sbjct: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI
Sbjct: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR
Sbjct: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 359

BLAST of Carg13727-RA vs. NCBI nr
Match: KAG6603747.1 (Transcription factor basic helix-loop-helix 112, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 734.6 bits (1895), Expect = 4.2e-208
Identity = 355/359 (98.89%), Postives = 356/359 (99.16%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
           QNSMFFQDYSTSDHWNQPHDL+LKRGDYLLEQNGVNCMNCCSSTSQGLFP DSALYGYPS
Sbjct: 61  QNSMFFQDYSTSDHWNQPHDLVLKRGDYLLEQNGVNCMNCCSSTSQGLFPPDSALYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYNNDDDDDD  HPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNNDDDDDDDDHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI
Sbjct: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR
Sbjct: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 359

BLAST of Carg13727-RA vs. NCBI nr
Match: XP_022950200.1 (transcription factor bHLH112-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 725.3 bits (1871), Expect = 2.5e-205
Identity = 352/359 (98.05%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
           QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS
Sbjct: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYNND+DDDD  HPDPHSLFNAATT INTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNNDNDDDD-DHPDPHSLFNAATTPINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPV KRPRI
Sbjct: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVLKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITP+QQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATT DFWSPTFGGTFR
Sbjct: 301 MKNNNGITPVQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTADFWSPTFGGTFR 358

BLAST of Carg13727-RA vs. NCBI nr
Match: XP_023544490.1 (transcription factor bHLH112-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 722.6 bits (1864), Expect = 1.6e-204
Identity = 350/359 (97.49%), Postives = 351/359 (97.77%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
           QNSMFFQDYSTSDHWNQPHD LLKRGDYLLEQNGVNCMN CSSTSQGLFPLDS LYGYPS
Sbjct: 61  QNSMFFQDYSTSDHWNQPHDFLLKRGDYLLEQNGVNCMN-CSSTSQGLFPLDSGLYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYNNDDDDD HHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNNDDDDDHHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWNASHAPPSIEIPQN RLGFKAMNNGNCEDGEEARKGDKNEPPV KRPRI
Sbjct: 181 ALHFTNNTPFWNASHAPPSIEIPQNCRLGFKAMNNGNCEDGEEARKGDKNEPPVLKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITP+Q QQVSEKADPKQDLKSRGLCLVPISSTFPVTNATT DFWSPTFGGTFR
Sbjct: 301 MKNNNGITPIQHQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTADFWSPTFGGTFR 358

BLAST of Carg13727-RA vs. NCBI nr
Match: XP_022950201.1 (transcription factor bHLH112-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 717.2 bits (1850), Expect = 6.9e-203
Identity = 350/359 (97.49%), Postives = 352/359 (98.05%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
           QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS
Sbjct: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYNND+DDDD  HPDPHSLFNAATT INTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNNDNDDDD-DHPDPHSLFNAATTPINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPV KRPRI
Sbjct: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVLKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQ  LLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQ--LLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITP+QQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATT DFWSPTFGGTFR
Sbjct: 301 MKNNNGITPVQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTADFWSPTFGGTFR 356

BLAST of Carg13727-RA vs. ExPASy Swiss-Prot
Match: Q94JL3 (Transcription factor bHLH112 OS=Arabidopsis thaliana OX=3702 GN=BHLH112 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 7.8e-48
Identity = 161/415 (38.80%), Postives = 211/415 (50.84%), Query Frame = 0

Query: 1   MAEDFQ--AAICGDN---WWSSSRTMLVAVGG-LSPCSMGIGDLAWPPADDFSDIKP--K 60
           MAE+F+  A+ICG     WW+S R+++      LSPC    G  A   ++DFS  +   K
Sbjct: 1   MAEEFKATASICGGGGGAWWNSPRSVMSPSDHFLSPC---FG--AAITSNDFSSQENHLK 60

Query: 61  STANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVN-CMNCCSSTSQGLFPL 120
           S    +DN N +F Q  + SD       +     D  L+  G+    NC S  +Q +   
Sbjct: 61  SRMTCTDNNNIVFGQREADSDSGGSTVTM-----DSTLQMMGLGFSSNCSSDWNQTILQE 120

Query: 121 DSALYGYPSPSTKLLHGLYNNDDDDDDHHH------PDPHSLFNAATTSINTNYPSNYLI 180
           D             L+  +     D DH          P+ L  A ++S +T+  S+ + 
Sbjct: 121 D-------------LNSSFIRSSQDQDHGQGFLSTTTSPYILNPACSSSPSTSSSSSLIR 180

Query: 181 KPSLPIPPPSQPVS--------PALHFTNNT---------------------PFWNAS-- 240
               P P P   VS        P L + N T                     PFWN+S  
Sbjct: 181 TFYDPEPSPYNFVSTTSGSINDPQLSWANKTNPHHQVAYGLINSFSNNANSRPFWNSSST 240

Query: 241 ----HAPPS--IEIPQ--NSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPL 300
               +  PS  +  PQ  ++RL  K  N       E  ++   NE   +K+PR+ TPSPL
Sbjct: 241 TNLNNTTPSNFVTTPQIISTRLEDKTKNLKTRAQSESLKRAKDNE-SAAKKPRVTTPSPL 300

Query: 301 PTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNG 357
           PTFKVRKE L D++T+LQQLVSPFGKTDTASVL EAIEYIKFLHDQV++LSTPYMK   G
Sbjct: 301 PTFKVRKENLRDQITSLQQLVSPFGKTDTASVLQEAIEYIKFLHDQVTVLSTPYMK--QG 360

BLAST of Carg13727-RA vs. ExPASy Swiss-Prot
Match: Q8GXT3 (Transcription factor bHLH123 OS=Arabidopsis thaliana OX=3702 GN=BHLH123 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.8e-45
Identity = 132/346 (38.15%), Postives = 179/346 (51.73%), Query Frame = 0

Query: 44  DDFSDIKPKSTANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSS 103
           D  SD KP+     +   N  FF D+  S H +   D            N  N M   ++
Sbjct: 131 DPQSDFKPQI---LTSGGNRGFFLDHQFSPHGSSSTDSSTVTCQGFAVDNSSNAMYAATT 190

Query: 104 TSQGLFPLDSALYGYPSPSTKLLH----GLYNNDDDDDDHHHPDP---HSLFNAATTSIN 163
           T+             P+ S+ + H    G + + D     +H      +S F ++T + +
Sbjct: 191 TT-------------PNSSSGMFHHQQAGGFGSSDQQPSRNHQQSSLGYSQFGSSTGNYD 250

Query: 164 ---TNYPSNYLIKPSLPIPPPSQPVSPALHFTNNTPFWN---------------ASHAPP 223
              +  PS + ++ S    PP +P SP L F+NN  FWN               +S+  P
Sbjct: 251 QMASALPSTWFLRSS----PPPKPHSP-LRFSNNATFWNPAAAGNAGAPPPHDASSNFFP 310

Query: 224 SIEIPQNSRLGF----KAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPLPTFKVRKE 283
           +++ PQ     F    K ++        E ++G  +  P +KR + E  SP P FK RKE
Sbjct: 311 ALQPPQIHPQSFDEQPKNISEIRDSSSNEVKRGGNDHQPAAKRAKSEAASPSPAFK-RKE 370

Query: 284 KLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNGITPLQQQQ 343
           K+GDR+ ALQQLVSPFGKTD ASVL EAIEYIKFLH QVS LS PYMK+   +   Q   
Sbjct: 371 KMGDRIAALQQLVSPFGKTDAASVLSEAIEYIKFLHQQVSALSNPYMKSGASLQHQQSDH 430

Query: 344 VSE-KADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
            +E +   + DL+SRGLCLVP+SSTFPVT+ TT DFW+PTFGGTFR
Sbjct: 431 STELEVSEEPDLRSRGLCLVPVSSTFPVTHDTTVDFWTPTFGGTFR 454

BLAST of Carg13727-RA vs. ExPASy Swiss-Prot
Match: Q9M0X8 (Transcription factor bHLH114 OS=Arabidopsis thaliana OX=3702 GN=BHLH114 PE=2 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-31
Identity = 82/153 (53.59%), Postives = 104/153 (67.97%), Query Frame = 0

Query: 210 FKAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPLPTFKVRKEKLGDRVTALQQLVSP 269
           +K + +   E+ EE    D+  P + KRPR+ET SPLP+FKVRKEKLGDR+TALQQLVSP
Sbjct: 135 YKNLTSKRSEELEE--NSDEYSPRLLKRPRLETLSPLPSFKVRKEKLGDRITALQQLVSP 194

Query: 270 FGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNGITPLQQQQVSEK----------- 329
           FGKTDTASVL+EA+EYIKFL +QV++LS P     N I  +QQQQ S K           
Sbjct: 195 FGKTDTASVLNEAVEYIKFLQEQVTVLSNP---EQNTIGSVQQQQCSNKKSINTQGEVEE 254

Query: 330 --ADPKQ--DLKSRGLCLVPISSTFPVTNATTT 348
               P++  DL SRGLCL+PIS+++PV  A  +
Sbjct: 255 DECSPRRYVDLSSRGLCLMPISASYPVAAAAAS 282

BLAST of Carg13727-RA vs. ExPASy Swiss-Prot
Match: Q8VZ22 (Transcription factor bHLH103 OS=Arabidopsis thaliana OX=3702 GN=BHLH103 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.5e-30
Identity = 74/129 (57.36%), Postives = 97/129 (75.19%), Query Frame = 0

Query: 236 KRPRIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVS- 295
           KRPR+ETPS  P+FKVRKEKLGDR+TALQQLVSPFGKTDTASVLH+AI+YIKFL +Q++ 
Sbjct: 176 KRPRLETPSHFPSFKVRKEKLGDRITALQQLVSPFGKTDTASVLHDAIDYIKFLQEQITE 235

Query: 296 -LLSTPYMKNNNGITPLQQQQVSEKA---------DPKQDLKSRGLCLVPISSTF--PVT 352
            + ++P++   N I   +Q+Q S+K+          P+QDL+SRGLCL+PISSTF  P  
Sbjct: 236 KVSTSPHL---NSIGSGEQKQWSDKSSNNTHNQNCSPRQDLRSRGLCLMPISSTFSTPPQ 295

BLAST of Carg13727-RA vs. ExPASy Swiss-Prot
Match: Q9SFZ3 (Transcription factor bHLH110 OS=Arabidopsis thaliana OX=3702 GN=BHLH110 PE=2 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 7.6e-27
Identity = 73/135 (54.07%), Postives = 85/135 (62.96%), Query Frame = 0

Query: 235 SKRPRIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVS 294
           SK+PR+E+ S  P FKVRKEKLGDR+ ALQQLVSPFGKTDTASVL EAI YIKFL  Q+ 
Sbjct: 317 SKKPRVESRSSCPPFKVRKEKLGDRIAALQQLVSPFGKTDTASVLMEAIGYIKFLQSQIE 376

Query: 295 LLSTPYMKNNNGITPLQQQQVSEK----ADPKQDLKSRGLCLVPISSTFPVT-------N 354
            LS PYM+ +        Q VS+      +  +DL+SRGLCLVP+S    VT        
Sbjct: 377 TLSVPYMRASRNRPGKASQLVSQSQEGDEEETRDLRSRGLCLVPLSCMTYVTGDGGDGGG 436

Query: 355 ATTTDFW--SPTFGG 357
              T FW   P FGG
Sbjct: 437 GVGTGFWPTPPGFGG 451

BLAST of Carg13727-RA vs. ExPASy TrEMBL
Match: A0A6J1GF19 (transcription factor bHLH112-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453364 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 1.2e-205
Identity = 352/359 (98.05%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
           QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS
Sbjct: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYNND+DDDD  HPDPHSLFNAATT INTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNNDNDDDD-DHPDPHSLFNAATTPINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPV KRPRI
Sbjct: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVLKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITP+QQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATT DFWSPTFGGTFR
Sbjct: 301 MKNNNGITPVQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTADFWSPTFGGTFR 358

BLAST of Carg13727-RA vs. ExPASy TrEMBL
Match: A0A6J1GE60 (transcription factor bHLH112-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453364 PE=4 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.3e-203
Identity = 350/359 (97.49%), Postives = 352/359 (98.05%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
           QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS
Sbjct: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYNND+DDDD  HPDPHSLFNAATT INTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNNDNDDDD-DHPDPHSLFNAATTPINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPV KRPRI
Sbjct: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVLKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQ  LLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQ--LLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITP+QQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATT DFWSPTFGGTFR
Sbjct: 301 MKNNNGITPVQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTADFWSPTFGGTFR 356

BLAST of Carg13727-RA vs. ExPASy TrEMBL
Match: A0A6J1IJP7 (transcription factor bHLH112-like OS=Cucurbita maxima OX=3661 GN=LOC111477714 PE=4 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 6.5e-199
Identity = 344/359 (95.82%), Postives = 347/359 (96.66%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN
Sbjct: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60

Query: 61  QNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120
            NSM FQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS
Sbjct: 61  HNSMVFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPS 120

Query: 121 PSTKLLHGLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSP 180
           PSTKLLHGLYN+DDDDD   HPDPHSLFN  TTSINTNYPSNYLIKPSLPIPPPSQPVSP
Sbjct: 121 PSTKLLHGLYNDDDDDD---HPDPHSLFN-TTTSINTNYPSNYLIKPSLPIPPPSQPVSP 180

Query: 181 ALHFTNNTPFWNASHAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRI 240
           ALHFTNNTPFWN SHAPPSIEIPQNSRLGFKAMNNGN EDGEEARKGDKNEPPV KRPRI
Sbjct: 181 ALHFTNNTPFWNTSHAPPSIEIPQNSRLGFKAMNNGNYEDGEEARKGDKNEPPVLKRPRI 240

Query: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300
           ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY
Sbjct: 241 ETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPY 300

Query: 301 MKNNNGITPLQQQQVSEKADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
           MKNNNGITP+Q QQVSEKA+PKQDLKSRGLCLVPISSTFPVTNATT DFWSPTFGGTFR
Sbjct: 301 MKNNNGITPVQHQQVSEKANPKQDLKSRGLCLVPISSTFPVTNATTADFWSPTFGGTFR 355

BLAST of Carg13727-RA vs. ExPASy TrEMBL
Match: A0A0A0KHH2 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G516730 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 6.5e-114
Identity = 255/429 (59.44%), Postives = 289/429 (67.37%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQA +CGDNWW+SSR+MLVAVGGLSPCS+GI DL+WPP       +P S A  S N
Sbjct: 1   MAEDFQAPVCGDNWWNSSRSMLVAVGGLSPCSVGIADLSWPP-------QPPSAATDSVN 60

Query: 61  QN-SMFFQDYS-------------TSDHWNQPHDLLLKRG-------DYLLEQ--NGVNC 120
            N SM FQDYS             TS+HWNQ  DLL   G       +  L+Q  +G + 
Sbjct: 61  NNSSMLFQDYSTFQMMGFALSSSTTSEHWNQSQDLLNSNGIPTNFSSENGLKQGNDGDDY 120

Query: 121 MNCCSSTSQ----GLFPLDSALYGYPSPSTKLLH-GLYNNDDDDDDHHHPDPHSLFNAAT 180
           +NC S+ +     GLFP++S+LYGY SPSTKLL   LY +D+        DPHSLFN +T
Sbjct: 121 LNCSSTNTTNCQGGLFPVESSLYGYSSPSTKLLQTNLYEDDNS-------DPHSLFN-ST 180

Query: 181 TSINT---------------------NYPSNYLIKPSLPIPPPSQPVSPALHFTNNTPFW 240
            SI T                     N+ SNYL+KPSLP P       P L FTNNTPFW
Sbjct: 181 PSITTNSTTFPTNINPPNHLSLSSTPNFSSNYLVKPSLPNP------QPHLQFTNNTPFW 240

Query: 241 NASHA---PPSIEI---------PQNSRLGFKAMNNGNCEDG-EEARKGDKN-----EPP 300
           NAS +   P  I            QN+ L FK++N    EDG ++ +KGDKN     EP 
Sbjct: 241 NASSSNSIPQGIASLLEDKNNPKSQNNTLAFKSVN----EDGQDQVKKGDKNSAITTEPT 300

Query: 301 VSKRPRIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQV 360
           V KRPRIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQV
Sbjct: 301 VFKRPRIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQV 360

BLAST of Carg13727-RA vs. ExPASy TrEMBL
Match: A0A6J1KL13 (transcription factor bHLH112-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496699 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 4.2e-113
Identity = 244/425 (57.41%), Postives = 264/425 (62.12%), Query Frame = 0

Query: 1   MAEDFQAAICGDNWWSSSRTMLVAVGGLSPCSMGIGDLAWPPADDFSDIKPKSTANYSDN 60
           MAEDFQAAICGDNWWS SR M+V VGGLSPCS+GIGDL WPPADDF DIKPKS  +   N
Sbjct: 1   MAEDFQAAICGDNWWSFSRNMMVTVGGLSPCSVGIGDLGWPPADDFLDIKPKSATDSDHN 60

Query: 61  QNSMFFQDYS-------------TSDHWNQPHDLL------------------------- 120
            NSM FQDYS             TSDHWNQP  LL                         
Sbjct: 61  NNSMLFQDYSTFQMMGFALSSSTTSDHWNQPDGLLCGGGRGESNFNSNGIERYKDEEITR 120

Query: 121 -----------LKRGD----YLLEQNGVNCMNCCSSTSQGLFPLDSALYGYPSPSTKLLH 180
                      LK+GD     +LEQNG+      + T QGLFPLDS LYGYP        
Sbjct: 121 SFTSDSSFINGLKQGDDEDYNMLEQNGL-MSTTNTPTCQGLFPLDSTLYGYP-------- 180

Query: 181 GLYNNDDDDDDHHHPDPHSLFNAATTSINTNYPSNYLIKPSLPIPPPSQPVSPALHFTNN 240
              +          P  HSLFN+                      P  QPVS ALHF NN
Sbjct: 181 ---HQQQQQQQPPPPHAHSLFNST--------------------HPQPQPVSAALHFANN 240

Query: 241 TPFWNASHAPPSIEIPQN-----SRLGFKAMNNGNCEDGEEARKGDKNEP----PVSKRP 300
           TPFWNA  A  S  IPQ       +   K +NNGNCE+G+E +KGDKN      PV KRP
Sbjct: 241 TPFWNAPTAASSTHIPQGIASLLEQKSAKPINNGNCENGQEPKKGDKNSANESVPVFKRP 300

Query: 301 RIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLST 360
           RIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVS+LST
Sbjct: 301 RIETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSVLST 360

BLAST of Carg13727-RA vs. TAIR 10
Match: AT1G61660.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 192.6 bits (488), Expect = 5.5e-49
Identity = 161/415 (38.80%), Postives = 211/415 (50.84%), Query Frame = 0

Query: 1   MAEDFQ--AAICGDN---WWSSSRTMLVAVGG-LSPCSMGIGDLAWPPADDFSDIKP--K 60
           MAE+F+  A+ICG     WW+S R+++      LSPC    G  A   ++DFS  +   K
Sbjct: 1   MAEEFKATASICGGGGGAWWNSPRSVMSPSDHFLSPC---FG--AAITSNDFSSQENHLK 60

Query: 61  STANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVN-CMNCCSSTSQGLFPL 120
           S    +DN N +F Q  + SD       +     D  L+  G+    NC S  +Q +   
Sbjct: 61  SRMTCTDNNNIVFGQREADSDSGGSTVTM-----DSTLQMMGLGFSSNCSSDWNQTILQE 120

Query: 121 DSALYGYPSPSTKLLHGLYNNDDDDDDHHH------PDPHSLFNAATTSINTNYPSNYLI 180
           D             L+  +     D DH          P+ L  A ++S +T+  S+ + 
Sbjct: 121 D-------------LNSSFIRSSQDQDHGQGFLSTTTSPYILNPACSSSPSTSSSSSLIR 180

Query: 181 KPSLPIPPPSQPVS--------PALHFTNNT---------------------PFWNAS-- 240
               P P P   VS        P L + N T                     PFWN+S  
Sbjct: 181 TFYDPEPSPYNFVSTTSGSINDPQLSWANKTNPHHQVAYGLINSFSNNANSRPFWNSSST 240

Query: 241 ----HAPPS--IEIPQ--NSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPL 300
               +  PS  +  PQ  ++RL  K  N       E  ++   NE   +K+PR+ TPSPL
Sbjct: 241 TNLNNTTPSNFVTTPQIISTRLEDKTKNLKTRAQSESLKRAKDNE-SAAKKPRVTTPSPL 300

Query: 301 PTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNG 357
           PTFKVRKE L D++T+LQQLVSPFGKTDTASVL EAIEYIKFLHDQV++LSTPYMK   G
Sbjct: 301 PTFKVRKENLRDQITSLQQLVSPFGKTDTASVLQEAIEYIKFLHDQVTVLSTPYMK--QG 360

BLAST of Carg13727-RA vs. TAIR 10
Match: AT1G61660.3 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.6e-48
Identity = 155/411 (37.71%), Postives = 206/411 (50.12%), Query Frame = 0

Query: 1   MAEDFQ--AAICGDN---WWSSSRTMLVAVGG-LSPCSMGIGDLAWPPADDFSDIKP--K 60
           MAE+F+  A+ICG     WW+S R+++      LSPC    G  A   ++DFS  +   K
Sbjct: 1   MAEEFKATASICGGGGGAWWNSPRSVMSPSDHFLSPC---FG--AAITSNDFSSQENHLK 60

Query: 61  STANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVN-CMNCCSSTSQGLFPL 120
           S    +DN N +F Q  + SD       +     D  L+  G+    NC S  +Q +   
Sbjct: 61  SRMTCTDNNNIVFGQREADSDSGGSTVTM-----DSTLQMMGLGFSSNCSSDWNQTILQE 120

Query: 121 DSALYGYPSPSTKLLHGLYNNDDDDDDHHH------PDPHSLFNAATTSINTNYPSNYLI 180
           D             L+  +     D DH          P+ L  A ++S +T+  S+ + 
Sbjct: 121 D-------------LNSSFIRSSQDQDHGQGFLSTTTSPYILNPACSSSPSTSSSSSLIR 180

Query: 181 KPSLPIPPPSQPVS--------PALHFTNNT---------------------PFWNAS-- 240
               P P P   VS        P L + N T                     PFWN+S  
Sbjct: 181 TFYDPEPSPYNFVSTTSGSINDPQLSWANKTNPHHQVAYGLINSFSNNANSRPFWNSSST 240

Query: 241 ----HAPPSIEIPQNSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPLPTFK 300
               +  PS  +     +  +  +        E+ K  K+    +K+PR+ TPSPLPTFK
Sbjct: 241 TNLNNTTPSNFVTTPQIISTRLEDKTKTRAQSESLKRAKDNESAAKKPRVTTPSPLPTFK 300

Query: 301 VRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNGITPL 357
           VRKE L D++T+LQQLVSPFGKTDTASVL EAIEYIKFLHDQV++LSTPYMK   G +  
Sbjct: 301 VRKENLRDQITSLQQLVSPFGKTDTASVLQEAIEYIKFLHDQVTVLSTPYMK--QGASNQ 360

BLAST of Carg13727-RA vs. TAIR 10
Match: AT3G20640.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.0e-46
Identity = 132/346 (38.15%), Postives = 179/346 (51.73%), Query Frame = 0

Query: 44  DDFSDIKPKSTANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVNCMNCCSS 103
           D  SD KP+     +   N  FF D+  S H +   D            N  N M   ++
Sbjct: 131 DPQSDFKPQI---LTSGGNRGFFLDHQFSPHGSSSTDSSTVTCQGFAVDNSSNAMYAATT 190

Query: 104 TSQGLFPLDSALYGYPSPSTKLLH----GLYNNDDDDDDHHHPDP---HSLFNAATTSIN 163
           T+             P+ S+ + H    G + + D     +H      +S F ++T + +
Sbjct: 191 TT-------------PNSSSGMFHHQQAGGFGSSDQQPSRNHQQSSLGYSQFGSSTGNYD 250

Query: 164 ---TNYPSNYLIKPSLPIPPPSQPVSPALHFTNNTPFWN---------------ASHAPP 223
              +  PS + ++ S    PP +P SP L F+NN  FWN               +S+  P
Sbjct: 251 QMASALPSTWFLRSS----PPPKPHSP-LRFSNNATFWNPAAAGNAGAPPPHDASSNFFP 310

Query: 224 SIEIPQNSRLGF----KAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPLPTFKVRKE 283
           +++ PQ     F    K ++        E ++G  +  P +KR + E  SP P FK RKE
Sbjct: 311 ALQPPQIHPQSFDEQPKNISEIRDSSSNEVKRGGNDHQPAAKRAKSEAASPSPAFK-RKE 370

Query: 284 KLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNGITPLQQQQ 343
           K+GDR+ ALQQLVSPFGKTD ASVL EAIEYIKFLH QVS LS PYMK+   +   Q   
Sbjct: 371 KMGDRIAALQQLVSPFGKTDAASVLSEAIEYIKFLHQQVSALSNPYMKSGASLQHQQSDH 430

Query: 344 VSE-KADPKQDLKSRGLCLVPISSTFPVTNATTTDFWSPTFGGTFR 360
            +E +   + DL+SRGLCLVP+SSTFPVT+ TT DFW+PTFGGTFR
Sbjct: 431 STELEVSEEPDLRSRGLCLVPVSSTFPVTHDTTVDFWTPTFGGTFR 454

BLAST of Carg13727-RA vs. TAIR 10
Match: AT1G61660.4 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 146.4 bits (368), Expect = 4.5e-35
Identity = 144/415 (34.70%), Postives = 191/415 (46.02%), Query Frame = 0

Query: 1   MAEDFQ--AAICGDN---WWSSSRTMLVAVGG-LSPCSMGIGDLAWPPADDFSDIKP--K 60
           MAE+F+  A+ICG     WW+S R+++      LSPC    G  A   ++DFS  +   K
Sbjct: 1   MAEEFKATASICGGGGGAWWNSPRSVMSPSDHFLSPC---FG--AAITSNDFSSQENHLK 60

Query: 61  STANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVN-CMNCCSSTSQGLFPL 120
           S    +DN N +F Q  + SD       +     D  L+  G+    NC S  +Q +   
Sbjct: 61  SRMTCTDNNNIVFGQREADSDSGGSTVTM-----DSTLQMMGLGFSSNCSSDWNQTILQE 120

Query: 121 DSALYGYPSPSTKLLHGLYNNDDDDDDHHH------PDPHSLFNAATTSINTNYPSNYLI 180
           D             L+  +     D DH          P+ L  A ++S +T+  S+ + 
Sbjct: 121 D-------------LNSSFIRSSQDQDHGQGFLSTTTSPYILNPACSSSPSTSSSSSLIR 180

Query: 181 KPSLPIPPPSQPVS--------PALHFTNNT---------------------PFWNAS-- 240
               P P P   VS        P L + N T                     PFWN+S  
Sbjct: 181 TFYDPEPSPYNFVSTTSGSINDPQLSWANKTNPHHQVAYGLINSFSNNANSRPFWNSSST 240

Query: 241 ----HAPPS--IEIPQ--NSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPL 300
               +  PS  +  PQ  ++RL  K  N       E  ++   NE   +K+PR+ TPSPL
Sbjct: 241 TNLNNTTPSNFVTTPQIISTRLEDKTKNLKTRAQSESLKRAKDNE-SAAKKPRVTTPSPL 300

Query: 301 PTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNG 357
           PTF                      KTDTASVL EAIEYIKFLHDQV++LSTPYMK   G
Sbjct: 301 PTF----------------------KTDTASVLQEAIEYIKFLHDQVTVLSTPYMK--QG 360

BLAST of Carg13727-RA vs. TAIR 10
Match: AT1G61660.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 141.7 bits (356), Expect = 1.1e-33
Identity = 135/369 (36.59%), Postives = 179/369 (48.51%), Query Frame = 0

Query: 1   MAEDFQ--AAICGDN---WWSSSRTMLVAVGG-LSPCSMGIGDLAWPPADDFSDIKP--K 60
           MAE+F+  A+ICG     WW+S R+++      LSPC    G  A   ++DFS  +   K
Sbjct: 1   MAEEFKATASICGGGGGAWWNSPRSVMSPSDHFLSPC---FG--AAITSNDFSSQENHLK 60

Query: 61  STANYSDNQNSMFFQDYSTSDHWNQPHDLLLKRGDYLLEQNGVN-CMNCCSSTSQGLFPL 120
           S    +DN N +F Q  + SD       +     D  L+  G+    NC S  +Q +   
Sbjct: 61  SRMTCTDNNNIVFGQREADSDSGGSTVTM-----DSTLQMMGLGFSSNCSSDWNQTILQE 120

Query: 121 DSALYGYPSPSTKLLHGLYNNDDDDDDHHH------PDPHSLFNAATTSINTNYPSNYLI 180
           D             L+  +     D DH          P+ L  A ++S +T+  S+ + 
Sbjct: 121 D-------------LNSSFIRSSQDQDHGQGFLSTTTSPYILNPACSSSPSTSSSSSLIR 180

Query: 181 KPSLPIPPPSQPVS--------PALHFTNNT---------------------PFWNAS-- 240
               P P P   VS        P L + N T                     PFWN+S  
Sbjct: 181 TFYDPEPSPYNFVSTTSGSINDPQLSWANKTNPHHQVAYGLINSFSNNANSRPFWNSSST 240

Query: 241 ----HAPPS--IEIPQ--NSRLGFKAMNNGNCEDGEEARKGDKNEPPVSKRPRIETPSPL 300
               +  PS  +  PQ  ++RL  K  N       E  ++   NE   +K+PR+ TPSPL
Sbjct: 241 TNLNNTTPSNFVTTPQIISTRLEDKTKNLKTRAQSESLKRAKDNE-SAAKKPRVTTPSPL 300

Query: 301 PTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKFLHDQVSLLSTPYMKNNNG 316
           PTFKVRKE L D++T+LQQLVSPFGKTDTASVL EAIEYIKFLHDQV++LSTPYMK   G
Sbjct: 301 PTFKVRKENLRDQITSLQQLVSPFGKTDTASVLQEAIEYIKFLHDQVTVLSTPYMK--QG 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7033917.12.4e-211100.00Transcription factor bHLH [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6603747.14.2e-20898.89Transcription factor basic helix-loop-helix 112, partial [Cucurbita argyrosperma... [more]
XP_022950200.12.5e-20598.05transcription factor bHLH112-like isoform X1 [Cucurbita moschata][more]
XP_023544490.11.6e-20497.49transcription factor bHLH112-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022950201.16.9e-20397.49transcription factor bHLH112-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q94JL37.8e-4838.80Transcription factor bHLH112 OS=Arabidopsis thaliana OX=3702 GN=BHLH112 PE=1 SV=... [more]
Q8GXT32.8e-4538.15Transcription factor bHLH123 OS=Arabidopsis thaliana OX=3702 GN=BHLH123 PE=1 SV=... [more]
Q9M0X81.3e-3153.59Transcription factor bHLH114 OS=Arabidopsis thaliana OX=3702 GN=BHLH114 PE=2 SV=... [more]
Q8VZ221.5e-3057.36Transcription factor bHLH103 OS=Arabidopsis thaliana OX=3702 GN=BHLH103 PE=1 SV=... [more]
Q9SFZ37.6e-2754.07Transcription factor bHLH110 OS=Arabidopsis thaliana OX=3702 GN=BHLH110 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1GF191.2e-20598.05transcription factor bHLH112-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1GE603.3e-20397.49transcription factor bHLH112-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IJP76.5e-19995.82transcription factor bHLH112-like OS=Cucurbita maxima OX=3661 GN=LOC111477714 PE... [more]
A0A0A0KHH26.5e-11459.44BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G516730 PE=4 S... [more]
A0A6J1KL134.2e-11357.41transcription factor bHLH112-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G61660.15.5e-4938.80basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G61660.33.6e-4837.71basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G20640.12.0e-4638.15basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G61660.44.5e-3534.70basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G61660.21.1e-3336.59basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 250..317
e-value: 1.6E-6
score: 30.1
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 250..307
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 218..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 212..247
NoneNo IPR availablePANTHERPTHR16223:SF248TRANSCRIPTION FACTOR BHLH112coord: 44..346
NoneNo IPR availablePANTHERPTHR16223TRANSCRIPTION FACTOR BHLH83-RELATEDcoord: 44..346
NoneNo IPR availableCDDcd11393bHLH_AtbHLH_likecoord: 246..297
e-value: 2.07143E-22
score: 86.8515
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 240..289
score: 11.667798

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg13727Carg13727gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13727-RA:7Carg13727-RA:7exon
Carg13727-RA:6Carg13727-RA:6exon
Carg13727-RA:5Carg13727-RA:5exon
Carg13727-RA:4Carg13727-RA:4exon
Carg13727-RA:3Carg13727-RA:3exon
Carg13727-RA:2Carg13727-RA:2exon
Carg13727-RA:1Carg13727-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13727-RA:three_prime_utrCarg13727-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13727-RA:cdsCarg13727-RA:cds_7CDS
Carg13727-RA:cdsCarg13727-RA:cds_6CDS
Carg13727-RA:cdsCarg13727-RA:cds_5CDS
Carg13727-RA:cdsCarg13727-RA:cds_4CDS
Carg13727-RA:cdsCarg13727-RA:cds_3CDS
Carg13727-RA:cdsCarg13727-RA:cds_2CDS
Carg13727-RA:cdsCarg13727-RA:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13727-RA:five_prime_utrCarg13727-RA:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg13727-RACarg13727-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity