Carg13608-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13608-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionseed biotin-containing protein SBP65-like
LocationCarg_Chr02: 419974 .. 421449 (-)
Sequence length1476
RNA-Seq ExpressionCarg13608-RA
SyntenyCarg13608-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACAGAGCAACTGAGCCGCAGAGATGACACCACGAAGGAAAAAGAGACTCAGGTGGAGAGAGAAAGAGTCCCACAATTGGCAAGCCACTTTGAGGCCATTGCTGTGCACGGCAAAACGACTGACCCCAGTAATGAAAAAGCTACAACACCAGAAGAAACGAAGGCGAGACAAAGGAGTGATGCTAGTGGAGTGGGAAAAGAGACGTATGGCACCGGCATTGCTAGAGGGACAGTTCAAGGAAGGGAGGAGGAGAGACAGGGAGTTGGAGGTGAGAAACCAGAAGACAGCCATGAATTGGCTGCTCAATTTGAGTCTCTTGCTGACAAAGTAAGAGACAAGAGAGAGACTAATACAGAGAATGAAAGGGAGAGGCAAGCACGTGCTGATAAAGAGAGAATTGCTGCTCGTGAGAGAGAGCAAAAAGGTCGAGCAGAGGAAAAACGGAGAGCTTCAGAACAGGCAAAAGAGAAAGAAGGTGAAATAGGATCTCGTCAAGATCAACCGACTCTTGAAGAAATTTCCAATTACAGAGCTATAGCACAGCAGAAATCGAACGAGGCAATAATGGCGGCAAAGGAACGGTATGACAAAGCAAACAAAAAATCACTCGAGAAAGGAGTTGAAGCTGGAACGGGGGAAGCAATACCGAGAGGCACAGAAGAAGCTGAGGACACAGTCGAAGAGGGAAATCAATATACAGGTTTAGAGACAGTGAAAGAGACGCTTACAAGTGCAGCCAAAACAGCAAAGGATTACACCGCGCCAATAGCAGAGAAGGCCAAAGATTACACAGTCTCTGCAGGTCGAACAACAGCTCATTACCTTGGGGAGAAGGCGGTGGTGGCTAAGGATGTTGCAATAGAAAGTGGGAAAGTTGCAGCTGAGTATGCAGGTAAAGCAGCAGAGGATTTGAAGGATAAGACGGCAGTTGCAGGGTGGAGCACGGCCCATTACTCATGTGACACGGCGGTGGAGGGGACTAAAGCAGCAGCAAGGCTCGTGAAGGGAGCTGCAGAGTACGCAGGAGCCATTGCAGCCAAGCCCTTGTCTGCTGCAAAGAATGTTGCTGAATCAACAGGCGAGACTGTGAAGGACTATACAGCCAGGAAGAAAGAAGAAGCACAGAGAGAATCAATGTACAAGCAAACTGAGAAAGAATCCATGTACGAAAAAACTAAGGAAACTTTTGAAGGGGGTATAGAAGAAGTGAAGGAAAGGGGAGGAGAAAGAGGTGAAGAGAACAGAGTTGAAGAGGATAGAAAAGATGGGGGGATATTAGGGGCAATTGGGGAGACCATATATGAGATTGCACAGACAACAAAAGAGATAGTGATTGGGCCTGGTGATGAAACTGGGAAACAGAGCACATTAGGCTTTGAGCTTGGTCAGGAAGAGGGTGAGGAAAAAGGCCAAGAACATGAAAAGAAAGAGCACTATGAAACTGCAGCCAAGAAGGAACACAGAGCCTGA

mRNA sequence

ATGGCGACAGAGCAACTGAGCCGCAGAGATGACACCACGAAGGAAAAAGAGACTCAGGTGGAGAGAGAAAGAGTCCCACAATTGGCAAGCCACTTTGAGGCCATTGCTGTGCACGGCAAAACGACTGACCCCAGTAATGAAAAAGCTACAACACCAGAAGAAACGAAGGCGAGACAAAGGAGTGATGCTAGTGGAGTGGGAAAAGAGACGTATGGCACCGGCATTGCTAGAGGGACAGTTCAAGGAAGGGAGGAGGAGAGACAGGGAGTTGGAGGTGAGAAACCAGAAGACAGCCATGAATTGGCTGCTCAATTTGAGTCTCTTGCTGACAAAGTAAGAGACAAGAGAGAGACTAATACAGAGAATGAAAGGGAGAGGCAAGCACGTGCTGATAAAGAGAGAATTGCTGCTCGTGAGAGAGAGCAAAAAGGTCGAGCAGAGGAAAAACGGAGAGCTTCAGAACAGGCAAAAGAGAAAGAAGGTGAAATAGGATCTCGTCAAGATCAACCGACTCTTGAAGAAATTTCCAATTACAGAGCTATAGCACAGCAGAAATCGAACGAGGCAATAATGGCGGCAAAGGAACGGTATGACAAAGCAAACAAAAAATCACTCGAGAAAGGAGTTGAAGCTGGAACGGGGGAAGCAATACCGAGAGGCACAGAAGAAGCTGAGGACACAGTCGAAGAGGGAAATCAATATACAGGTTTAGAGACAGTGAAAGAGACGCTTACAAGTGCAGCCAAAACAGCAAAGGATTACACCGCGCCAATAGCAGAGAAGGCCAAAGATTACACAGTCTCTGCAGGTCGAACAACAGCTCATTACCTTGGGGAGAAGGCGGTGGTGGCTAAGGATGTTGCAATAGAAAGTGGGAAAGTTGCAGCTGAGTATGCAGGTAAAGCAGCAGAGGATTTGAAGGATAAGACGGCAGTTGCAGGGTGGAGCACGGCCCATTACTCATGTGACACGGCGGTGGAGGGGACTAAAGCAGCAGCAAGGCTCGTGAAGGGAGCTGCAGAGTACGCAGGAGCCATTGCAGCCAAGCCCTTGTCTGCTGCAAAGAATGTTGCTGAATCAACAGGCGAGACTGTGAAGGACTATACAGCCAGGAAGAAAGAAGAAGCACAGAGAGAATCAATGTACAAGCAAACTGAGAAAGAATCCATGTACGAAAAAACTAAGGAAACTTTTGAAGGGGGTATAGAAGAAGTGAAGGAAAGGGGAGGAGAAAGAGGTGAAGAGAACAGAGTTGAAGAGGATAGAAAAGATGGGGGGATATTAGGGGCAATTGGGGAGACCATATATGAGATTGCACAGACAACAAAAGAGATAGTGATTGGGCCTGGTGATGAAACTGGGAAACAGAGCACATTAGGCTTTGAGCTTGGTCAGGAAGAGGGTGAGGAAAAAGGCCAAGAACATGAAAAGAAAGAGCACTATGAAACTGCAGCCAAGAAGGAACACAGAGCCTGA

Coding sequence (CDS)

ATGGCGACAGAGCAACTGAGCCGCAGAGATGACACCACGAAGGAAAAAGAGACTCAGGTGGAGAGAGAAAGAGTCCCACAATTGGCAAGCCACTTTGAGGCCATTGCTGTGCACGGCAAAACGACTGACCCCAGTAATGAAAAAGCTACAACACCAGAAGAAACGAAGGCGAGACAAAGGAGTGATGCTAGTGGAGTGGGAAAAGAGACGTATGGCACCGGCATTGCTAGAGGGACAGTTCAAGGAAGGGAGGAGGAGAGACAGGGAGTTGGAGGTGAGAAACCAGAAGACAGCCATGAATTGGCTGCTCAATTTGAGTCTCTTGCTGACAAAGTAAGAGACAAGAGAGAGACTAATACAGAGAATGAAAGGGAGAGGCAAGCACGTGCTGATAAAGAGAGAATTGCTGCTCGTGAGAGAGAGCAAAAAGGTCGAGCAGAGGAAAAACGGAGAGCTTCAGAACAGGCAAAAGAGAAAGAAGGTGAAATAGGATCTCGTCAAGATCAACCGACTCTTGAAGAAATTTCCAATTACAGAGCTATAGCACAGCAGAAATCGAACGAGGCAATAATGGCGGCAAAGGAACGGTATGACAAAGCAAACAAAAAATCACTCGAGAAAGGAGTTGAAGCTGGAACGGGGGAAGCAATACCGAGAGGCACAGAAGAAGCTGAGGACACAGTCGAAGAGGGAAATCAATATACAGGTTTAGAGACAGTGAAAGAGACGCTTACAAGTGCAGCCAAAACAGCAAAGGATTACACCGCGCCAATAGCAGAGAAGGCCAAAGATTACACAGTCTCTGCAGGTCGAACAACAGCTCATTACCTTGGGGAGAAGGCGGTGGTGGCTAAGGATGTTGCAATAGAAAGTGGGAAAGTTGCAGCTGAGTATGCAGGTAAAGCAGCAGAGGATTTGAAGGATAAGACGGCAGTTGCAGGGTGGAGCACGGCCCATTACTCATGTGACACGGCGGTGGAGGGGACTAAAGCAGCAGCAAGGCTCGTGAAGGGAGCTGCAGAGTACGCAGGAGCCATTGCAGCCAAGCCCTTGTCTGCTGCAAAGAATGTTGCTGAATCAACAGGCGAGACTGTGAAGGACTATACAGCCAGGAAGAAAGAAGAAGCACAGAGAGAATCAATGTACAAGCAAACTGAGAAAGAATCCATGTACGAAAAAACTAAGGAAACTTTTGAAGGGGGTATAGAAGAAGTGAAGGAAAGGGGAGGAGAAAGAGGTGAAGAGAACAGAGTTGAAGAGGATAGAAAAGATGGGGGGATATTAGGGGCAATTGGGGAGACCATATATGAGATTGCACAGACAACAAAAGAGATAGTGATTGGGCCTGGTGATGAAACTGGGAAACAGAGCACATTAGGCTTTGAGCTTGGTCAGGAAGAGGGTGAGGAAAAAGGCCAAGAACATGAAAAGAAAGAGCACTATGAAACTGCAGCCAAGAAGGAACACAGAGCCTGA

Protein sequence

MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQRSDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNTENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETVKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEEDRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEHYETAAKKEHRA
Homology
BLAST of Carg13608-RA vs. NCBI nr
Match: KAG7034893.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 878.6 bits (2269), Expect = 2.5e-251
Identity = 491/491 (100.00%), Postives = 491/491 (100.00%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
           SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120

Query: 121 ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180
           ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA
Sbjct: 121 ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180

Query: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV 240
           IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV
Sbjct: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV 240

Query: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300
           KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG
Sbjct: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300

Query: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360
           KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES
Sbjct: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360

Query: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420
           TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE
Sbjct: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420

Query: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH 480
           DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH
Sbjct: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH 480

Query: 481 YETAAKKEHRA 492
           YETAAKKEHRA
Sbjct: 481 YETAAKKEHRA 491

BLAST of Carg13608-RA vs. NCBI nr
Match: XP_022947224.1 (seed biotin-containing protein SBP65-like [Cucurbita moschata])

HSP 1 Score: 875.9 bits (2262), Expect = 1.6e-250
Identity = 490/491 (99.80%), Postives = 490/491 (99.80%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
           SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120

Query: 121 ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180
           ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA
Sbjct: 121 ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180

Query: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV 240
           IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV
Sbjct: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV 240

Query: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300
           KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG
Sbjct: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300

Query: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360
           KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES
Sbjct: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360

Query: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420
           TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE
Sbjct: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420

Query: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH 480
           DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEH KKEH
Sbjct: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHVKKEH 480

Query: 481 YETAAKKEHRA 492
           YETAAKKEHRA
Sbjct: 481 YETAAKKEHRA 491

BLAST of Carg13608-RA vs. NCBI nr
Match: KAG6604765.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 875.2 bits (2260), Expect = 2.7e-250
Identity = 489/491 (99.59%), Postives = 489/491 (99.59%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
           SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120

Query: 121 ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180
           ENERERQARADKERIAAREREQKGR EEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA
Sbjct: 121 ENERERQARADKERIAAREREQKGREEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180

Query: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV 240
           IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLET 
Sbjct: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETA 240

Query: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300
           KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG
Sbjct: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300

Query: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360
           KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES
Sbjct: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360

Query: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420
           TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE
Sbjct: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420

Query: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH 480
           DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH
Sbjct: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH 480

Query: 481 YETAAKKEHRA 492
           YETAAKKEHRA
Sbjct: 481 YETAAKKEHRA 491

BLAST of Carg13608-RA vs. NCBI nr
Match: XP_023534218.1 (seed biotin-containing protein SBP65-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.8 bits (2202), Expect = 1.4e-243
Identity = 479/492 (97.36%), Postives = 486/492 (98.78%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MATE+LSRRDDTTKEKETQVERERVPQLASHFEAIAVHGK TDPSNEKATTPEETKARQR
Sbjct: 1   MATEKLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKATDPSNEKATTPEETKARQR 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
           SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120

Query: 121 ENERERQARADKERIAAREREQKG-RAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYR 180
           ENERERQARADKERIAAREREQKG RAEEK+R SEQAKEKEGEIGSRQ+QP+LEEISNYR
Sbjct: 121 ENERERQARADKERIAAREREQKGSRAEEKQRVSEQAKEKEGEIGSRQNQPSLEEISNYR 180

Query: 181 AIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLET 240
           AIAQQKSNEAIMAAKERYDKANKKSL++GVEAGTGEAIPRGTEEAEDTVEEGNQYTGLET
Sbjct: 181 AIAQQKSNEAIMAAKERYDKANKKSLQQGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLET 240

Query: 241 VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYA 300
           VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAA YA
Sbjct: 241 VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAGYA 300

Query: 301 GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE 360
           GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE
Sbjct: 301 GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE 360

Query: 361 STGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVE 420
           STGETVKDYTARKKEEAQRESMYKQTEK+SMYEKTKETFEGGIEEVKERGGERG ENRVE
Sbjct: 361 STGETVKDYTARKKEEAQRESMYKQTEKDSMYEKTKETFEGGIEEVKERGGERGGENRVE 420

Query: 421 EDRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKE 480
           EDR DGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKE
Sbjct: 421 EDRSDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKE 480

Query: 481 HYETAAKKEHRA 492
           HYETAAKKEHRA
Sbjct: 481 HYETAAKKEHRA 492

BLAST of Carg13608-RA vs. NCBI nr
Match: XP_022970889.1 (seed biotin-containing protein SBP65-like [Cucurbita maxima])

HSP 1 Score: 840.1 bits (2169), Expect = 9.7e-240
Identity = 473/492 (96.14%), Postives = 483/492 (98.17%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MATE+LSRRDDTTKEKE QVERERVPQL SHFEAIAVHGK TDPSNEKATTPEETKARQ+
Sbjct: 1   MATEKLSRRDDTTKEKEIQVERERVPQLTSHFEAIAVHGKATDPSNEKATTPEETKARQK 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
           SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRET+T
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETDT 120

Query: 121 ENERERQARADKERIAAREREQKG-RAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYR 180
           ENERERQARADKERIAAREREQKG RAEEK+RA EQAKE+EGEIGSRQ+QP+LEEISNYR
Sbjct: 121 ENERERQARADKERIAAREREQKGSRAEEKQRALEQAKEREGEIGSRQNQPSLEEISNYR 180

Query: 181 AIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLET 240
           AIAQQKSNEAIMAAKERYDKANKKSLE+GVEAGT EAIPRGTEEAEDTVEEGNQY+GLET
Sbjct: 181 AIAQQKSNEAIMAAKERYDKANKKSLEQGVEAGTREAIPRGTEEAEDTVEEGNQYSGLET 240

Query: 241 VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYA 300
           VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYA
Sbjct: 241 VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYA 300

Query: 301 GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE 360
           GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE
Sbjct: 301 GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE 360

Query: 361 STGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVE 420
           STGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFE GIEEVKERGGERGEENRVE
Sbjct: 361 STGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEEGIEEVKERGGERGEENRVE 420

Query: 421 EDRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKE 480
           EDR DGGILGAIGETIYEIAQTTK+IVIGPGDETGKQSTLGFELGQEE EEKGQEHEKKE
Sbjct: 421 EDRNDGGILGAIGETIYEIAQTTKDIVIGPGDETGKQSTLGFELGQEEDEEKGQEHEKKE 480

Query: 481 HYETAAKKEHRA 492
           HYETAAKKEHRA
Sbjct: 481 HYETAAKKEHRA 492

BLAST of Carg13608-RA vs. ExPASy Swiss-Prot
Match: Q39846 (Seed biotin-containing protein SBP65 OS=Glycine max OX=3847 GN=SBP65 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 7.4e-49
Identity = 204/569 (35.85%), Postives = 283/569 (49.74%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MA+EQL+RR++TT EKE  VE+ RVP++A+HFE +A   K +D +  K T     +A Q 
Sbjct: 1   MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESDITAGKDTPQGSIEALQA 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
            +     +     G A G + GR + R         ++HEL A FESLADKV D    N 
Sbjct: 61  GE-----RVKDHAGKAMGDIGGRGKAR---------ETHELGAHFESLADKVTDHAAANV 120

Query: 121 ENERERQARA----------------------DKERIAAREREQKGRAE----------- 180
              +E Q  A                      D+  +  R RE  GR E           
Sbjct: 121 VGNKESQREARGGVRDVGKFEMRTEGGEKGNKDRPELKTRTREVIGRTEKERGRESGGQV 180

Query: 181 --EKRRASEQAK-----EKEG------------------------EIGSRQDQPTLEEIS 240
             EK R +E A+     E EG                        E  S  ++   E+IS
Sbjct: 181 VAEKGRETETARGRVGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQIS 240

Query: 241 NYRAIAQQKSNEAIMAAKERYDKANKKSLE------KGVEAGTGEAIPRGTEEAEDTVEE 300
           NY       S++A    KE+Y++A + + E      +  +  + +A     +  + T+E+
Sbjct: 241 NY-------SDQATQGVKEKYERAKQAASETLNTTTQTAQEKSAQAKNLAAQAKDATLEK 300

Query: 301 GNQYTGLETVKETLTSAAKTAKDYTAPIAEKAKDYTV-------SAGRTTAHYLGEKAVV 360
           G Q  G    K+T++SAAKTA + TAP+AEKAKDYT+       SAG TTA Y+GEKAV 
Sbjct: 301 GQQ--GYAVTKDTISSAAKTASEKTAPVAEKAKDYTLQAAEKAKSAGGTTASYVGEKAVQ 360

Query: 361 AKDVAIESGKVAAEYAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYA 420
           AKDVA+ESGK AA YA K A DL+DK    GW+ AH+S +  VEGTKAAA +V+GAA YA
Sbjct: 361 AKDVAVESGKSAAGYAAKVAADLRDKATAVGWAAAHFSAEKTVEGTKAAAHVVEGAAGYA 420

Query: 421 GAIAAKPLS----AAKNVAESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFE 475
           G  AA+  S    A K +A S GET K+YTA+KKEEAQRE    + +K S  ++ +E   
Sbjct: 421 GHKAAELASMSAGAVKGLAASAGETAKEYTAKKKEEAQRE---LEAKKPSQPQEAEERPS 480

BLAST of Carg13608-RA vs. ExPASy Swiss-Prot
Match: Q41060 (Seed biotin-containing protein SBP65 OS=Pisum sativum OX=3888 GN=SBP65 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.2e-28
Identity = 170/550 (30.91%), Postives = 238/550 (43.27%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTD----PSNE-KATTPEET 60
           MA+EQLSRR++ T E++ Q   + VPQ  +HFE    H    +    P NE +A      
Sbjct: 1   MASEQLSRRENITTERKIQNAEDSVPQRTTHFELRETHELGPNFQSLPRNENQAYLDRGA 60

Query: 61  KARQRSDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDK 120
           +A   ++ S    +     +     + R  E++  GG +         Q ES        
Sbjct: 61  RAPLSANVSESYLDRARVPLNANIPEHRVREKEDFGGVRDMGKF----QMESKGGNKSLA 120

Query: 121 RETNTENERERQARADKERIAAREREQKGR-AEEKRRASEQAKEKEGEIGSRQDQPTLEE 180
            +  T + R R           +E   KG+  EE+ RA E+A E+E      + + T+EE
Sbjct: 121 EDRETLDTRSRMVTGTPH---IKEASGKGQVVEERERARERAMEEE------EKRLTMEE 180

Query: 181 ISNYRAIAQQKSNEAIMAAKERYDKA-----------NKKSLEKGVEA----GTGEAIPR 240
           IS YR  AQQ + EA+ AA+E+Y++A            + + EKG  A     T E   +
Sbjct: 181 ISKYRNQAQQSALEALSAAQEKYERAKQATNETLRNTTQAAQEKGEAAQAKDATFEKTQQ 240

Query: 241 GTEEAEDTVEE-----------------GNQYTGLETVKETLTSAAKTAKDYTAPIAEK- 300
           G E   DTV                   G    G E  ++T+++AA+TA +Y  P AEK 
Sbjct: 241 GYEMTGDTVSNSARTASEKAAQAKNTTLGKTQQGYEATRDTVSNAARTAAEYATPAAEKA 300

Query: 301 ------AKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKDKTAVAGW 360
                 AKD T+  G+T A               E  K AAE A K A DLK+K  VAGW
Sbjct: 301 RCVAVQAKDVTLETGKTAA---------------EKAKCAAEIAAKVAVDLKEKATVAGW 360

Query: 361 STAHYSCDTAVEGTKAAARLVKGAAEY----AGAIAAKPLSAAKNVAESTGETVKDYTAR 420
           + +HY+    V+GT+AAA  V+GA  Y    A  +AAK +   K +A S GET K++TAR
Sbjct: 361 TASHYATQLTVDGTRAAANAVEGAVGYVAPKASELAAKSVETVKGLAASAGETAKEFTAR 420

Query: 421 KKEEAQRESMYKQTE--------------------------------KESMYEKTKETFE 456
           KKEE+ RE   K+                                  +E +  K  +   
Sbjct: 421 KKEESWREYEAKRASQLQEGEEILPSTGGIGKVLPSGERTQAQGTNLQEKVQGKGSDILG 480

BLAST of Carg13608-RA vs. ExPASy TrEMBL
Match: A0A6J1G664 (seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC111451152 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.7e-251
Identity = 490/491 (99.80%), Postives = 490/491 (99.80%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
           SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120

Query: 121 ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180
           ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA
Sbjct: 121 ENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYRA 180

Query: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV 240
           IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV
Sbjct: 181 IAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETV 240

Query: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300
           KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG
Sbjct: 241 KETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAG 300

Query: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360
           KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES
Sbjct: 301 KAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAES 360

Query: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420
           TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE
Sbjct: 361 TGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVEE 420

Query: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEH 480
           DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEH KKEH
Sbjct: 421 DRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHVKKEH 480

Query: 481 YETAAKKEHRA 492
           YETAAKKEHRA
Sbjct: 481 YETAAKKEHRA 491

BLAST of Carg13608-RA vs. ExPASy TrEMBL
Match: A0A6J1I6Z1 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111469726 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.7e-240
Identity = 473/492 (96.14%), Postives = 483/492 (98.17%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MATE+LSRRDDTTKEKE QVERERVPQL SHFEAIAVHGK TDPSNEKATTPEETKARQ+
Sbjct: 1   MATEKLSRRDDTTKEKEIQVERERVPQLTSHFEAIAVHGKATDPSNEKATTPEETKARQK 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETNT 120
           SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRET+T
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETDT 120

Query: 121 ENERERQARADKERIAAREREQKG-RAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNYR 180
           ENERERQARADKERIAAREREQKG RAEEK+RA EQAKE+EGEIGSRQ+QP+LEEISNYR
Sbjct: 121 ENERERQARADKERIAAREREQKGSRAEEKQRALEQAKEREGEIGSRQNQPSLEEISNYR 180

Query: 181 AIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLET 240
           AIAQQKSNEAIMAAKERYDKANKKSLE+GVEAGT EAIPRGTEEAEDTVEEGNQY+GLET
Sbjct: 181 AIAQQKSNEAIMAAKERYDKANKKSLEQGVEAGTREAIPRGTEEAEDTVEEGNQYSGLET 240

Query: 241 VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYA 300
           VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYA
Sbjct: 241 VKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYA 300

Query: 301 GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE 360
           GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE
Sbjct: 301 GKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAE 360

Query: 361 STGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRVE 420
           STGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFE GIEEVKERGGERGEENRVE
Sbjct: 361 STGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEEGIEEVKERGGERGEENRVE 420

Query: 421 EDRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKE 480
           EDR DGGILGAIGETIYEIAQTTK+IVIGPGDETGKQSTLGFELGQEE EEKGQEHEKKE
Sbjct: 421 EDRNDGGILGAIGETIYEIAQTTKDIVIGPGDETGKQSTLGFELGQEEDEEKGQEHEKKE 480

Query: 481 HYETAAKKEHRA 492
           HYETAAKKEHRA
Sbjct: 481 HYETAAKKEHRA 492

BLAST of Carg13608-RA vs. ExPASy TrEMBL
Match: A0A6J1JER8 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111483844 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.7e-157
Identity = 356/507 (70.22%), Postives = 393/507 (77.51%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MA EQLSRRD+TTKE+E QVE+E+VPQL SHFEAIAV          KA TPE+T     
Sbjct: 1   MAAEQLSRRDNTTKEREIQVEKEKVPQLTSHFEAIAVQC--------KAATPEKT----H 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQ----GVGGEKPEDSHELAAQFESLADKVRDKR 120
             AS  GKE  GT IA+G + GRE  R      VGG KP+D+HELAAQF+SLA+KVRDKR
Sbjct: 61  ELASDGGKEAQGTSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKR 120

Query: 121 ETNTENERERQARADKERIAAREREQKG-RAEEKRRASEQAKEKEGEIGSRQDQPTLEEI 180
           ET+TENER+R+ARADK      EREQKG R  E++R SEQ K KEG+IGSRQ+QP+LEEI
Sbjct: 121 ETDTENERKRRARADK------EREQKGSRIGEEQRLSEQKKGKEGDIGSRQNQPSLEEI 180

Query: 181 SNYRAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYT 240
           SN RA  Q+KSN+A MAAKERYD+A K+SL +GV A T E+  RGTE AEDTVEE  Q T
Sbjct: 181 SNVRATEQEKSNKATMAAKERYDEAKKESLNQGVGARTEESDQRGTEGAEDTVEEKGQQT 240

Query: 241 GLETVKETLTSAAKTAKDYTAPIAEKAKDY-----------TVSAGRTTAHYLGEKAVVA 300
           GL  V+ETLTSAAKTAKDYT PIAEKAKDY           TV+ G+TTAHYLGEKAV A
Sbjct: 241 GLGAVEETLTSAAKTAKDYTVPIAEKAKDYTVQKAVEAKDITVTVGQTTAHYLGEKAVAA 300

Query: 301 KDVAIESGKVAAEYAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAG 360
           KDV I  GKVAAEYAGKA EDLKDK  VAGWSTAHYSCDTAVEGTKAAARLVKGAAE AG
Sbjct: 301 KDVVIGGGKVAAEYAGKATEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAG 360

Query: 361 AIAAKPLSAAKNVAESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEE 420
           AIAAKPLSAAK VAESTGE+VK+YTARKKEEAQRES YKQ EKESMYEKTKETFEG  EE
Sbjct: 361 AIAAKPLSAAKTVAESTGESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREE 420

Query: 421 VKERGGERGEENRVEEDRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELG 480
           VKERG  R  E    E R DGGILGAIGETIYEIAQTTKEIVIG GDET KQ+T  FE G
Sbjct: 421 VKERGEGRVNEKNAAEGRDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKQNTFDFEHG 480

Query: 481 QEEGEEKGQEHEKKEHYETAAKKEHRA 492
           +EEGE   Q + + EHY+TA +KE+RA
Sbjct: 481 EEEGEANDQGYGRGEHYQTATRKEYRA 489

BLAST of Carg13608-RA vs. ExPASy TrEMBL
Match: A0A5A7ULM4 (Seed biotin-containing protein SBP65 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G002010 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 3.2e-156
Identity = 346/504 (68.65%), Postives = 397/504 (78.77%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MA+EQLSRRD+TT E+E QVE+ERVPQL SHFEAIA  G        KAT PE+T+ +QR
Sbjct: 1   MASEQLSRRDNTTNEREIQVEKERVPQLTSHFEAIAGQG--------KATIPEKTEEKQR 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGE--KPEDSHELAAQFESLADKVRDKRET 120
           S ASG  KE +G  IA+G + GREEER+ V GE  KP ++HELA+QFESLA KVRD RET
Sbjct: 61  SGASGGRKEVHGISIAKGELPGREEERKRVEGEGGKPNETHELASQFESLAQKVRDNRET 120

Query: 121 NTENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNY 180
           +TENER+RQ R +++R++            K+R SEQAKEKEGEIG+RQ+QP+L+EISNY
Sbjct: 121 DTENERQRQDRENEQRVS------------KQRDSEQAKEKEGEIGTRQNQPSLDEISNY 180

Query: 181 RAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAED-TVEEGNQYTGL 240
           RAIAQ++SNEAI AAKERY+KANK+SL +GVE  T E+  R TEE E+ TVE G + TGL
Sbjct: 181 RAIAQERSNEAIRAAKERYEKANKESLHQGVEGKTEESEQRETEEGEENTVEVGGERTGL 240

Query: 241 ETVKETLTSAAKTAKDYTAPIAEKAKDY-----------TVSAGRTTAHYLGEKAVVAKD 300
           ETVKETLTSAAKTAKDYT PIAEKAKDY           TVSAG+TTAHYLGEKAV AKD
Sbjct: 241 ETVKETLTSAAKTAKDYTVPIAEKAKDYTVQKAIEAKDITVSAGQTTAHYLGEKAVAAKD 300

Query: 301 VAIESGKVAAEYAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAI 360
           VA+ESGKVAAEYAGKAAEDLKDK  VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAI
Sbjct: 301 VAVESGKVAAEYAGKAAEDLKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAI 360

Query: 361 AAKPLSAAKNVAESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVK 420
           A KPLSAAKNVA+STGE+VK+YTARKKE A+RE+ YK  EKE+MYEKTK TFEGG EE+K
Sbjct: 361 AGKPLSAAKNVAQSTGESVKEYTARKKEVAEREATYKTGEKETMYEKTKGTFEGGKEELK 420

Query: 421 ERGGERGEENRVEEDRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQE 480
            RG E+    RV++   DGGILGAIGETIYEIAQTTKEIVIG GDE+GKQS LGFE+G+E
Sbjct: 421 GRGNEK----RVDD---DGGILGAIGETIYEIAQTTKEIVIGDGDESGKQSKLGFEIGEE 465

Query: 481 EGEEKGQEHEKKEHYETAAKKEHR 491
                       E YET  +KE R
Sbjct: 481 ------------EQYETDTRKEQR 465

BLAST of Carg13608-RA vs. ExPASy TrEMBL
Match: A0A1S3C372 (seed biotin-containing protein SBP65 OS=Cucumis melo OX=3656 GN=LOC103496368 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 4.2e-156
Identity = 346/504 (68.65%), Postives = 397/504 (78.77%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60
           MA+EQLSRRD+TT E+E QVE+ERVPQL SHFEAIA  G        KAT PE+T+ +QR
Sbjct: 1   MASEQLSRRDNTTNEREIQVEKERVPQLTSHFEAIAGQG--------KATIPEKTEEKQR 60

Query: 61  SDASGVGKETYGTGIARGTVQGREEERQGVGGE--KPEDSHELAAQFESLADKVRDKRET 120
           S ASG  KE +G  IA+G + GREEER+ V GE  KP ++HELA+QFESLA KVRD RET
Sbjct: 61  SGASGGRKEVHGISIAKGELPGREEERKRVEGEGGKPNETHELASQFESLAQKVRDNRET 120

Query: 121 NTENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNY 180
           +TENER+RQ R +++R++            K+R SEQAKEKEGEIG+RQ+QP+L+EISNY
Sbjct: 121 DTENERQRQDRENEQRVS------------KQRDSEQAKEKEGEIGTRQNQPSLDEISNY 180

Query: 181 RAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAED-TVEEGNQYTGL 240
           RAIAQ++SNEAI AAKERY+KANK+SL +GVE  T E+  R TEE E+ TVE G + TGL
Sbjct: 181 RAIAQERSNEAIRAAKERYEKANKESLHQGVEGKTEESEQRETEEGEENTVEVGGERTGL 240

Query: 241 ETVKETLTSAAKTAKDYTAPIAEKAKDY-----------TVSAGRTTAHYLGEKAVVAKD 300
           ETVKETLTSAAKTAKDYT PIAEKAKDY           TVSAG+TTAHYLGEKAV AKD
Sbjct: 241 ETVKETLTSAAKTAKDYTVPIAEKAKDYTVQKAIEAKDITVSAGQTTAHYLGEKAVAAKD 300

Query: 301 VAIESGKVAAEYAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAI 360
           VA+ESGKVAAEYAGKAAEDLKDK  VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAI
Sbjct: 301 VAVESGKVAAEYAGKAAEDLKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAI 360

Query: 361 AAKPLSAAKNVAESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVK 420
           A KPLSAAKNVA+STGE+VK+YTARKKE A+RE+ YK  EKE+MYEKTK TFEGG EE+K
Sbjct: 361 AGKPLSAAKNVAQSTGESVKEYTARKKEIAEREATYKTGEKETMYEKTKGTFEGGKEELK 420

Query: 421 ERGGERGEENRVEEDRKDGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQE 480
            RG E+    RV++   DGGILGAIGETIYEIAQTTKEIVIG GDE+GKQS LGFE+G+E
Sbjct: 421 GRGNEK----RVDD---DGGILGAIGETIYEIAQTTKEIVIGDGDESGKQSKLGFEIGEE 465

Query: 481 EGEEKGQEHEKKEHYETAAKKEHR 491
                       E YET  +KE R
Sbjct: 481 ------------EQYETDTRKEQR 465

BLAST of Carg13608-RA vs. TAIR 10
Match: AT2G42560.1 (late embryogenesis abundant domain-containing protein / LEA domain-containing protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.5e-20
Identity = 176/620 (28.39%), Postives = 252/620 (40.65%), Query Frame = 0

Query: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDP---------------S 60
           MA+EQ +RR++   E+E QVE++RVP++ SHFE++A  GK +D                S
Sbjct: 1   MASEQ-ARRENKVTEREVQVEKDRVPKMTSHFESMAEKGKDSDTHRHQTEGGGTQFVSLS 60

Query: 61  NEKATTP------EETKARQRSDASGVGKETYGTGIARGTVQGREEERQGVGGEKPEDSH 120
           ++ +  P       ETK ++      VGK    +    G  +  +EE +       ED H
Sbjct: 61  DKGSNMPVSDEGEGETKMKRTQMPHSVGKFVTSSDSGTGKKKDEKEEHEKA---SLEDIH 120

Query: 121 ELAAQFESLADKVRDKRETNTENERERQARADKERIAAREREQKGRAE------------ 180
              A  +  +       E      +E  + + +E    R  E  G+              
Sbjct: 121 GYRANAQQKSMDSIKAAEERYNKAKESLSHSGQEARGGRGEEMVGKGRDSGVRVSHVGAV 180

Query: 181 ------------------EKRRASE------------QAKEKEGEIGSRQDQPTL-EEIS 240
                             EK R +E            + KE  G +G R  + T+ E+  
Sbjct: 181 GGGGGGEEKESGVHGFHGEKARHAELLAAGGEEMREREGKESAGGVGGRSVKDTVAEKGQ 240

Query: 241 NYRAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAG--TGEAIPRGTE-------EAEDT 300
             +    + + +A  A  E+  +A++ + EKG EAG  T E   R  +       EA++T
Sbjct: 241 QAKESVGEGAQKAGSATSEKAQRASEYATEKGKEAGNMTAEQAARAKDYALQKAVEAKET 300

Query: 301 VEEGNQYTGLETVKETLTSAAK---TAKDYTAPIAEKAKDYTVSAGRTTAHYL------- 360
             E  Q    E +KET ++AA+    AKDYT   A +AKD      +  + Y+       
Sbjct: 301 AAEKAQRAS-EYMKETGSTAAEQAARAKDYTLQKAVEAKDVAAEKAQRASEYMTETGKQA 360

Query: 361 --------------------------GEKAVVAKDVAIESGKVAAEYAGKAAEDLKDKTA 420
                                     GE A   K+  +E GK AA YAG AAE    K A
Sbjct: 361 GNVAAQKGQEAASMTAKAKDYTVQKAGEAAGYIKETTVEGGKGAAHYAGVAAE----KAA 420

Query: 421 VAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAG----AIAAKPLSAAKNVAESTGETVKD 449
             GW+ AH++ +  V+GTKA A  V+GA  YAG     + +K +   K  A    +TV  
Sbjct: 421 AVGWTAAHFTTEKVVQGTKAVAGTVEGAVGYAGHKAVEVGSKAVDLTKEKAAVAADTVVG 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034893.12.5e-251100.00Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. arg... [more]
XP_022947224.11.6e-25099.80seed biotin-containing protein SBP65-like [Cucurbita moschata][more]
KAG6604765.12.7e-25099.59Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023534218.11.4e-24397.36seed biotin-containing protein SBP65-like [Cucurbita pepo subsp. pepo][more]
XP_022970889.19.7e-24096.14seed biotin-containing protein SBP65-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q398467.4e-4935.85Seed biotin-containing protein SBP65 OS=Glycine max OX=3847 GN=SBP65 PE=2 SV=1[more]
Q410604.2e-2830.91Seed biotin-containing protein SBP65 OS=Pisum sativum OX=3888 GN=SBP65 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G6647.7e-25199.80seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1I6Z14.7e-24096.14seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1JER81.7e-15770.22seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A5A7ULM43.2e-15668.65Seed biotin-containing protein SBP65 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C3724.2e-15668.65seed biotin-containing protein SBP65 OS=Cucumis melo OX=3656 GN=LOC103496368 PE=... [more]
Match NameE-valueIdentityDescription
AT2G42560.11.5e-2028.39late embryogenesis abundant domain-containing protein / LEA domain-containing pr... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..203
NoneNo IPR availableCOILSCoilCoilcoord: 129..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 464..491
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 447..491
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 371..425
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..63
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..235
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..175
NoneNo IPR availablePANTHERPTHR47877:SF3LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN / LEA DOMAIN-CONTAINING PROTEINcoord: 1..479
NoneNo IPR availablePANTHERPTHR47877FAMILY NOT NAMEDcoord: 1..479

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg13608Carg13608gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13608-RA:1Carg13608-RA:1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg13608-RA:cdsCarg13608-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg13608-RACarg13608-RA-proteinpolypeptide