Carg12867-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12867-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	zinc transporter 11
Location	Carg_Chr09: 5613211 .. 5615864 (-)
Sequence length	1199
RNA-Seq Expression	Carg12867-RA
Synteny	Carg12867-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATCCCCTCCAAATGGCCCGCCGTCTCCTCCTCCTCTCCATTTTCCTCCTCCTCCTCTCCTCCGCCGTCGCCCACAGCGGCCACCACGACGATGACGATGCCGGAGAGGTCGAGGCCACTCCAAATCTCCGCTCTAAGCCGCTCATTCTCGTTAAGATCGGCTGTCTTATTCTCATCTTTTTCGGTACTTTCATCCCTGGAATCTCTCCCTGTTTCTTCAAATGGAACGATGGATTCCTTGTCCTTGGTACTCAGTTTGCTGGCGGCGTCTTCTTTGGCACTGCTATGATGCATTTCCTCTCCGATGCTAATGAGACGTTTGGCGATTTGACTGATAAGGCGTATCCGTTCGCGTTTATGCTTGCTTGTGTAGGGTTTTTGATGACTATGGCGGCGGATTGTGTGATATCGTATCTCTATCGGAAACAGGACGGTGATTCTTCGATTGATGTTGAGATTCGAGGTAATATTGATTCTAGAACTCTTCTGATTTGAAGTTTAATCTGGAATTTTTGTTCTGTTCTTCTGTGAAAATTGATTGTGGTTCTTCTTTCTTGTGAACTTCAAATTCGTTTTTTCGTGGTACTGATTCTGAATCGGCTAACAAACCGATGAATTTTCCTTCCAACTTTTCTGGATTCTTTGATTTTATCGATTTTCGTGGATTGAAATGAATGTGGAATAGAGAATCAAAGAGGATATTTATTTGCAATTTCACGCGATTGAGTAATTTCCTGAACTTGTCGGAACCAGAATCTGAGGAAACAACCAAAAGTAAAATAAGACAAAATTAATGATGAACGAATTAACTATGAACTTAGAGGTTGGGTTGGGGATTTTGAAAATCGCTTTCTAAAAGATGTCTTCCTTTTCCTTTATCCGTTTTCATACCAATGAATTTAGTGGTTGGGAAAGAACAAATGAATTAACTAATCAAATTTCAACACAGTTTTTTTGTTTTCTAACAAAAAAATATTAAATAATTAAAAACACTATTCTACTTTCCTTTTTTTAATTTTTACATTAGGTGTAAGTGGGTGGACTCATTTATTTGATTTCTAAACTTTAAAAATACATCCTTCACCTTTGTCAATTTTATAAATAGTTGATCCATTCAAAAACATTTTTACAAATTATAATGATTTCATTTGTCACAACTTTTTAGTATGATTTTTGAAATTTTGCTAGTTTGGACAGCGAAAGTTAATTTTTTTTAGTTCAACTTTTTAGTAAATATGTGAGATTTTAGGTTGGTTGGGGAGGGGCACCAAGAATTTCTTGTAGATTTGAGGCTGACGACGATACATGACGAGCCAAAGCGTACAATATTTATTAGCTGTGGAATTTGGTTGTTACAAATGGTATTAGAGCCAGACACCGGGTGGTGTGCGAGTGTGGAATTTGGTTGTTACAAATGGTATTAGAACCAGACACCGGGCGGTGTGCGAGTGAGAATGTTGGACCTAATGGGGGTGGATTGTGAGATCCCACGTAGGTTGGAGAGGGGAACGAACCATTCCTTATTAAGGGTGTGAAAACTGTGAGGCTGATGGTGATACGTAATGAGCCAAAGCAGATAAAATCTACACGCAGTGGAATTTGACTGTTACAAATGGTATCAGAGCCAGCACGCCCTTAAGAGGGTGAATTGTGAGATCCCACGTTGGTTGTAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAATCTCTCCCTAGTAGACTCGTCTTCAAATCGTGAGACAGACGGCTACGTAACGGACCAAAATAGACAATATTTACTAACGGTAGAATTTGGCTACGATCTTTATACAACCCTCAATAATTATGGTGTTCTTTCACTGCAGGAGCTGCAATTTCACCTTCAAAGCTTCAGGTAACAAAGCTAAACTCAGCTATCCTTCCTTGTTATATCCAACTTGAACTCATTTCTTTTGTGTAATTCAGGTCCATAATGGCAGCAATGGCCATAACCCACATCCACATGCAGCTATCACAACAATGGGTTCATTTGGGGACAGCATTTTACTAATCGTTGCGTTGTGCTTTCACTCTGTGTTCGAAGGCATTGCCATCGGCGTTGCAGAGACCGAAGCCGATGCCTGGAGAGCCCTATGGACAATCTCCCTCCACAAGGTTTTTGCAGCCATTGCCATGGGCATTGCTCTCCTCCGAATGATCCCAAACCGCCCCCTGTTGTCGAGTGCTGCCTACTCCTTTGCTTTCGCCATCTCGAGCCCCATTGGCATCGCTATCGGACTCATAATCGACGCCACGACAGAAGGCGCGGTTGCAGATTGGATCTTTGCGATCTCGATGGGGTTGGCTTGTGGAGTGTTCATTTATGTGTCCATAAACCATCTGCTTTCAAAGGGATACACAGCCAAGGATTCAGTTATGGTGGACAATCCCAATCACAAGTTTGTTGCTGTGCTTTTAGGAATTGGGATTATTGCTGTTGTAATGATTTGGGACACCTGATTCCCAATCATAGACGGAGAATATATCTATTTTAGATCAGAGCTTTTGTTGTAATGTAAAGTTAAAAGCGCTTTTTGCAGCCGAGTTTTACTCGGTCACATGTGTCTTACAATTTCTAGTAAGAGACCAAAACGAGAAAGAAGCTTATTTGAACGTTCTCATGATGTTTGAC

mRNA sequence

AAATCCCCTCCAAATGGCCCGCCGTCTCCTCCTCCTCTCCATTTTCCTCCTCCTCCTCTCCTCCGCCGTCGCCCACAGCGGCCACCACGACGATGACGATGCCGGAGAGGTCGAGGCCACTCCAAATCTCCGCTCTAAGCCGCTCATTCTCGTTAAGATCGGCTGTCTTATTCTCATCTTTTTCGGTACTTTCATCCCTGGAATCTCTCCCTGTTTCTTCAAATGGAACGATGGATTCCTTGTCCTTGGTACTCAGTTTGCTGGCGGCGTCTTCTTTGGCACTGCTATGATGCATTTCCTCTCCGATGCTAATGAGACGTTTGGCGATTTGACTGATAAGGCGTATCCGTTCGCGTTTATGCTTGCTTGTGTAGGGTTTTTGATGACTATGGCGGCGGATTGTGTGATATCGTATCTCTATCGGAAACAGGACGGTGATTCTTCGATTGATGTTGAGATTCGAGGAGCTGCAATTTCACCTTCAAAGCTTCAGGTCCATAATGGCAGCAATGGCCATAACCCACATCCACATGCAGCTATCACAACAATGGGTTCATTTGGGGACAGCATTTTACTAATCGTTGCGTTGTGCTTTCACTCTGTGTTCGAAGGCATTGCCATCGGCGTTGCAGAGACCGAAGCCGATGCCTGGAGAGCCCTATGGACAATCTCCCTCCACAAGGTTTTTGCAGCCATTGCCATGGGCATTGCTCTCCTCCGAATGATCCCAAACCGCCCCCTGTTGTCGAGTGCTGCCTACTCCTTTGCTTTCGCCATCTCGAGCCCCATTGGCATCGCTATCGGACTCATAATCGACGCCACGACAGAAGGCGCGGTTGCAGATTGGATCTTTGCGATCTCGATGGGGTTGGCTTGTGGAGTGTTCATTTATGTGTCCATAAACCATCTGCTTTCAAAGGGATACACAGCCAAGGATTCAGTTATGGTGGACAATCCCAATCACAAGTTTGTTGCTGTGCTTTTAGGAATTGGGATTATTGCTGTTGTAATGATTTGGGACACCTGATTCCCAATCATAGACGGAGAATATATCTATTTTAGATCAGAGCTTTTGTTGTAATGTAAAGTTAAAAGCGCTTTTTGCAGCCGAGTTTTACTCGGTCACATGTGTCTTACAATTTCTAGTAAGAGACCAAAACGAGAAAGAAGCTTATTTGAACGTTCTCATGATGTTTGAC

Coding sequence (CDS)

ATGGCCCGCCGTCTCCTCCTCCTCTCCATTTTCCTCCTCCTCCTCTCCTCCGCCGTCGCCCACAGCGGCCACCACGACGATGACGATGCCGGAGAGGTCGAGGCCACTCCAAATCTCCGCTCTAAGCCGCTCATTCTCGTTAAGATCGGCTGTCTTATTCTCATCTTTTTCGGTACTTTCATCCCTGGAATCTCTCCCTGTTTCTTCAAATGGAACGATGGATTCCTTGTCCTTGGTACTCAGTTTGCTGGCGGCGTCTTCTTTGGCACTGCTATGATGCATTTCCTCTCCGATGCTAATGAGACGTTTGGCGATTTGACTGATAAGGCGTATCCGTTCGCGTTTATGCTTGCTTGTGTAGGGTTTTTGATGACTATGGCGGCGGATTGTGTGATATCGTATCTCTATCGGAAACAGGACGGTGATTCTTCGATTGATGTTGAGATTCGAGGAGCTGCAATTTCACCTTCAAAGCTTCAGGTCCATAATGGCAGCAATGGCCATAACCCACATCCACATGCAGCTATCACAACAATGGGTTCATTTGGGGACAGCATTTTACTAATCGTTGCGTTGTGCTTTCACTCTGTGTTCGAAGGCATTGCCATCGGCGTTGCAGAGACCGAAGCCGATGCCTGGAGAGCCCTATGGACAATCTCCCTCCACAAGGTTTTTGCAGCCATTGCCATGGGCATTGCTCTCCTCCGAATGATCCCAAACCGCCCCCTGTTGTCGAGTGCTGCCTACTCCTTTGCTTTCGCCATCTCGAGCCCCATTGGCATCGCTATCGGACTCATAATCGACGCCACGACAGAAGGCGCGGTTGCAGATTGGATCTTTGCGATCTCGATGGGGTTGGCTTGTGGAGTGTTCATTTATGTGTCCATAAACCATCTGCTTTCAAAGGGATACACAGCCAAGGATTCAGTTATGGTGGACAATCCCAATCACAAGTTTGTTGCTGTGCTTTTAGGAATTGGGATTATTGCTGTTGTAATGATTTGGGACACCTGA

Protein sequence

MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTFIPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACVGFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLLSKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT

Homology

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BLAST of Carg12867-RA vs. NCBI nr
Match: KAG7024965.1 (Zinc transporter 11 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 662.5 bits (1708), Expect = 1.9e-186
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60
           MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF
Sbjct: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60

Query: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120
           IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV
Sbjct: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120

Query: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180
           GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG
Sbjct: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180

Query: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240
           SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN
Sbjct: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240

Query: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300
           RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL
Sbjct: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300

Query: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT
Sbjct: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 337

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BLAST of Carg12867-RA vs. NCBI nr
Match: KAG6592088.1 (Zinc transporter 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 660.2 bits (1702), Expect = 9.4e-186
Identity = 335/337 (99.41%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60
           MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF
Sbjct: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60

Query: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120
           IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV
Sbjct: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120

Query: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180
           GFLMTMAADCVISYLYRKQDGDSSIDVE+RGAAISPSKLQVHNGSNGHNPHPHAAITTMG
Sbjct: 121 GFLMTMAADCVISYLYRKQDGDSSIDVELRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180

Query: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240
           SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN
Sbjct: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240

Query: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300
           RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL
Sbjct: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300

Query: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           SKGYTAKDSV+VDNPNHKFVAVLLGIGIIAVVMIWDT
Sbjct: 301 SKGYTAKDSVVVDNPNHKFVAVLLGIGIIAVVMIWDT 337

[Close]

BLAST of Carg12867-RA vs. NCBI nr
Match: XP_023535779.1 (zinc transporter 11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.5 bits (1695), Expect = 6.1e-185
Identity = 334/337 (99.11%), Postives = 336/337 (99.70%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60
           MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF
Sbjct: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60

Query: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120
           IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV
Sbjct: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120

Query: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180
           GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG
Sbjct: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180

Query: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240
           SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN
Sbjct: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240

Query: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300
           RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL
Sbjct: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300

Query: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           SKG+T KDSV+VDNPNHKFVAVLLGIGIIAVVMIWDT
Sbjct: 301 SKGHTTKDSVVVDNPNHKFVAVLLGIGIIAVVMIWDT 337

[Close]

BLAST of Carg12867-RA vs. NCBI nr
Match: XP_022937302.1 (zinc transporter 11 [Cucurbita moschata])

HSP 1 Score: 649.4 bits (1674), Expect = 1.7e-182
Identity = 331/337 (98.22%), Postives = 333/337 (98.81%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60
           MAR LLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF
Sbjct: 1   MARHLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60

Query: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120
           IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV
Sbjct: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120

Query: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180
           GFLMTMAADCVISYLYRKQ+GDSSIDVE+RGAAISPSKLQVHNGSNGHNPHPHAAITTMG
Sbjct: 121 GFLMTMAADCVISYLYRKQNGDSSIDVELRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180

Query: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240
           SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN
Sbjct: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240

Query: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300
           RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL
Sbjct: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300

Query: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           SKGYT KDSV V NPNHKFVAVLLGIGIIAVVMIWDT
Sbjct: 301 SKGYTPKDSVGVVNPNHKFVAVLLGIGIIAVVMIWDT 337

[Close]

BLAST of Carg12867-RA vs. NCBI nr
Match: XP_022976274.1 (zinc transporter 11 [Cucurbita maxima])

HSP 1 Score: 640.6 bits (1651), Expect = 7.7e-180
Identity = 326/337 (96.74%), Postives = 328/337 (97.33%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60
           MAR LLL    LLLLSSAVAHSGHHDDDDAG+ EATPNLRSKPLILVKIGCLILIFFGTF
Sbjct: 1   MARHLLLSIFLLLLLSSAVAHSGHHDDDDAGDAEATPNLRSKPLILVKIGCLILIFFGTF 60

Query: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120
           IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV
Sbjct: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120

Query: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180
           GFLMTMAADCVISYLYRKQDGDSSIDVE+RG AISPSKLQVHNGSNGHNPHPHAAITTMG
Sbjct: 121 GFLMTMAADCVISYLYRKQDGDSSIDVELRGTAISPSKLQVHNGSNGHNPHPHAAITTMG 180

Query: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240
           SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN
Sbjct: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240

Query: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300
           RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL
Sbjct: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300

Query: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           SKGYTAKDSV V NPNHKFVAVLLGIGIIAVVMIWDT
Sbjct: 301 SKGYTAKDSVGVVNPNHKFVAVLLGIGIIAVVMIWDT 337

[Close]

BLAST of Carg12867-RA vs. ExPASy Swiss-Prot
Match: Q94EG9 (Zinc transporter 11 OS=Arabidopsis thaliana OX=3702 GN=ZIP11 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 6.3e-108
Identity = 205/347 (59.08%), Postives = 255/347 (73.49%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAH--SGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFG 60
           M+R L+   +FL+L+   ++H   G HDDD+A  V+++ +L+SK LI VKI CL++IF  
Sbjct: 1   MSRSLVFFFLFLVLVVPCLSHGTGGDHDDDEASHVKSS-DLKSKSLISVKIACLVIIFVL 60

Query: 61  TFIPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDL--------TDKA 120
           TFI G+SP F KW+ GFLVLGTQFAGGVF  TA+MHFLSDA+ETF  L           A
Sbjct: 61  TFISGVSPYFLKWSQGFLVLGTQFAGGVFLATALMHFLSDADETFRGLLTAEGESEPSPA 120

Query: 121 YPFAFMLACVGFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNP 180
           YPFA+MLAC GF++TM AD VI+++Y K   D  +                     G + 
Sbjct: 121 YPFAYMLACAGFMLTMLADSVIAHIYSKTQNDLEL--------------------QGEDK 180

Query: 181 HPHAAITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAM 240
               + TT  S GDSILLIVALCFHSVFEGIAIG++ET++DAWRALWTI+LHK+FAAIAM
Sbjct: 181 SNQRSATTETSIGDSILLIVALCFHSVFEGIAIGISETKSDAWRALWTITLHKIFAAIAM 240

Query: 241 GIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGV 300
           GIALLRMIP+RPL SS  YSFAFAISSPIG+AIG++IDATT+G++ADWIFA+SM LACGV
Sbjct: 241 GIALLRMIPDRPLFSSITYSFAFAISSPIGVAIGIVIDATTQGSIADWIFALSMSLACGV 300

Query: 301 FIYVSINHLLSKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           F+YVS+NHLL+KGY     V VD P +KF+AVL G+ +IA+VMIWDT
Sbjct: 301 FVYVSVNHLLAKGYRPNKKVHVDEPRYKFLAVLFGVVVIAIVMIWDT 326

[Close]

BLAST of Carg12867-RA vs. ExPASy Swiss-Prot
Match: Q852F6 (Zinc transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP2 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.2e-103
Identity = 194/325 (59.69%), Postives = 235/325 (72.31%), Query Frame = 0


Query: 23  GHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTFIPGISPCFFKWNDGFLVLGTQF 82
           G  D D  G  E  P+LR++ L+  K+ CL ++F GT   G+SP F +WND FL LGTQF
Sbjct: 35  GDSDADADGGGEGKPDLRARGLVAAKLWCLAVVFAGTLAGGVSPYFMRWNDAFLALGTQF 94

Query: 83  AGGVFFGTAMMHFLSDANETFGD-LTDKAYPFAFMLACVGFLMTMAADCVISYLYRKQDG 142
           AGGVF GTAMMHFL+DANETF D L   AYPFAFMLAC G+++TM ADC IS++  +  G
Sbjct: 95  AGGVFLGTAMMHFLADANETFADLLPGTAYPFAFMLACAGYVLTMLADCAISFVVARGGG 154

Query: 143 DSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAA---------ITTMGSFGDSILLIVAL 202
            +       GA +   KL   NG+    P   AA         +    + GDS+LLI AL
Sbjct: 155 RTE-PAAAAGAGLEEGKLSSTNGNASDPPAADAAAQDHSVASMLRNASTLGDSVLLIAAL 214

Query: 203 CFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPNRPLLSSAAYSFA 262
           CFHSVFEGIAIGVAET+ADAW+ALWTISLHK+FAAIAMGIALLRM+P+RP LS   Y+FA
Sbjct: 215 CFHSVFEGIAIGVAETKADAWKALWTISLHKIFAAIAMGIALLRMLPDRPFLSCFGYAFA 274

Query: 263 FAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLLSKGYTAKDSVMV 322
           FA+SSP+G+ IG++IDATT+G VADWIFA+SMGLA G+FIYVSINHLLSKGYT    V  
Sbjct: 275 FAVSSPVGVGIGIVIDATTQGRVADWIFAVSMGLATGIFIYVSINHLLSKGYTPLRPVAA 334

Query: 323 DNPNHKFVAVLLGIGIIAVVMIWDT 338
           D P  + +AV+LG+ +IAVVMIWDT
Sbjct: 335 DTPAGRLLAVVLGVAVIAVVMIWDT 358

[Close]

BLAST of Carg12867-RA vs. ExPASy Swiss-Prot
Match: Q9LTH9 (Zinc transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ZIP2 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 6.1e-95
Identity = 181/349 (51.86%), Postives = 242/349 (69.34%), Query Frame = 0


Query: 5   LLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATP---------NLRSKPLILVKIGCLILI 64
           L  LSI  L  S  +AH G  D D+  E    P         NLRSK L+LVKI C+I++
Sbjct: 12  LFFLSIIFLCFSLILAHGGIDDGDEEEETNQPPPATGTTTVVNLRSKSLVLVKIYCIIIL 71

Query: 65  FFGTFIPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAF 124
           FF TF+ G+SP F++WN+ FL+LGTQF+GG+F  TA++HFLSDANETF  L  K YP+AF
Sbjct: 72  FFSTFLAGVSPYFYRWNESFLLLGTQFSGGIFLATALIHFLSDANETFRGLKHKEYPYAF 131

Query: 125 MLACVGFLMTMAADCVISYLYRKQD--------GDSSIDVEIRGAAISPSKLQVHNGSNG 184
           MLA  G+ +TM AD  ++++    +        G+S  D ++  A     + ++ +G + 
Sbjct: 132 MLAAAGYCLTMLADVAVAFVAAGSNNNHVGASVGESREDDDV--AVKEEGRREIKSGVD- 191

Query: 185 HNPHPHAAITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAA 244
                 A I T G FGD+ LLI ALCFHS+FEGIAIG+++T++DAWR LWTISLHKVFAA
Sbjct: 192 ---VSQALIRTSG-FGDTALLIFALCFHSIFEGIAIGLSDTKSDAWRNLWTISLHKVFAA 251

Query: 245 IAMGIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLA 304
           +AMGIALL++IP RP   +  YSFAF ISSPIG+ IG+ I+AT++GA  DW +AISMGLA
Sbjct: 252 VAMGIALLKLIPKRPFFLTVVYSFAFGISSPIGVGIGIGINATSQGAGGDWTYAISMGLA 311

Query: 305 CGVFIYVSINHLLSKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWD 337
           CGVF+YV++NHL+SKGY  ++    D P +KF+AV LG+ +++VVMIWD
Sbjct: 312 CGVFVYVAVNHLISKGYKPREECYFDKPIYKFIAVFLGVALLSVVMIWD 353

[Close]

BLAST of Carg12867-RA vs. ExPASy Swiss-Prot
Match: Q94DG6 (Zinc transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP1 PE=1 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.1e-91
Identity = 179/342 (52.34%), Postives = 234/342 (68.42%), Query Frame = 0


Query: 6   LLLSIFLLLLSSAVAHSGH---HDDDDAGEVEATP----NLRSKPLILVKIGCLILIFFG 65
           LL+++ LL   S  A SGH   +D D   +  ATP     +RSK LI VK+ CL+++   
Sbjct: 12  LLVAVVLLAALSFQACSGHGGINDGDGQVDAPATPASSSGVRSKGLIAVKVWCLVILLVF 71

Query: 66  TFIPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLA 125
           TF  G+SP F++WN+ FL+LGTQFA GVF GTA+MHFL+D+  TF  LT   YPF+FML 
Sbjct: 72  TFAGGVSPYFYRWNESFLLLGTQFAAGVFLGTALMHFLADSTSTFKGLTTNQYPFSFMLT 131

Query: 126 CVGFLMTMAADCVISYLYRKQD----GDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHA 185
           CVGFL+TM +D VI+ + R+       D+ +  + +      + +           HP A
Sbjct: 132 CVGFLLTMLSDLVIAAVARRSAAAGVSDNQVSEQQQRQQAEGAVMSRKEEEAAAVAHP-A 191

Query: 186 AITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIAL 245
            +    SF D++LLIVALCFHSVFEGIAIGV+ ++++AWR LWTI LHK+FAA+AMGIAL
Sbjct: 192 MLVRTSSFEDAVLLIVALCFHSVFEGIAIGVSASKSEAWRNLWTIGLHKIFAAVAMGIAL 251

Query: 246 LRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYV 305
           LRMIP RP L +  YS AFA+SSP+G+ IG+ IDAT++G  ADW +AISMGLA GVFIYV
Sbjct: 252 LRMIPKRPFLMTVVYSLAFAVSSPVGVGIGIAIDATSQGRAADWTYAISMGLATGVFIYV 311

Query: 306 SINHLLSKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWD 337
           +INHL++KGY        D P  KF+AVLLG+ ++AVVMIWD
Sbjct: 312 AINHLIAKGYRPHHPTAADKPLFKFLAVLLGVAVMAVVMIWD 352

[Close]

BLAST of Carg12867-RA vs. ExPASy Swiss-Prot
Match: P59889 (Zinc transporter ZIP1 OS=Danio rerio OX=7955 GN=slc39a1 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 8.1e-15
Identity = 84/311 (27.01%), Postives = 136/311 (43.73%), Query Frame = 0


Query: 44  LILVKIGCLILIFFGTFIPGISPCFFKW---------NDGFLVLGTQFAGGVFFGTAMMH 103
           L+ VK+G L+ +   T   G  P   KW         +   L   + FAGGVF    ++ 
Sbjct: 4   LLQVKVGALVGLLLLTLFFGFIPARMKWFHVTGGTELHKAVLSFVSCFAGGVFLSACLLD 63

Query: 104 FLSD-ANETFGDL----TDKAYPF-AFMLACVGFLMTMAADCVISYLYRKQDGDSSIDVE 163
            + D  ++  G+L     D  +P   F++AC  F + +    V+S     ++ +++    
Sbjct: 64  IIPDYLSDIHGELQKRDLDDGFPLPEFIMACGFFTVLILEKMVLSCTEGHRNEETA---- 123

Query: 164 IRGAAISPSKLQVH-------NGSNGHNPHPHAAITTMGSFGDSILLIVALCFHSVFEGI 223
                ++P+    H       N   G   H H       SF  S +L ++L  HSVFEG+
Sbjct: 124 ---PLLAPAAPNGHAHGHPSVNDLEGSGHHVHVDFHAHSSF-RSFMLFLSLSLHSVFEGL 183

Query: 224 AIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPNRPLLSSAAYSFAFAISSPIGI 283
           AIG+  T A        I +HK     ++ + L++    +PL     Y   FAI SP+GI
Sbjct: 184 AIGLQTTNAKVLEICIAILVHKSIIVFSLSVKLVQSAV-KPLW-VVLYVTVFAIMSPLGI 243

Query: 284 AIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLLSKGYTAKDSVMVDNPNHKFVA 333
            IG+++   TE      I A+  GLA G FIY++   +L     +      + P  K + 
Sbjct: 244 GIGIVV-IETERQAGGLIQAVLEGLAAGTFIYITFLEILPHELNSS-----ERPLLKVLF 298

[Close]

BLAST of Carg12867-RA vs. ExPASy TrEMBL
Match: A0A6J1FAT9 (zinc transporter 11 OS=Cucurbita moschata OX=3662 GN=LOC111443628 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 8.0e-183
Identity = 331/337 (98.22%), Postives = 333/337 (98.81%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60
           MAR LLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF
Sbjct: 1   MARHLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60

Query: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120
           IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV
Sbjct: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120

Query: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180
           GFLMTMAADCVISYLYRKQ+GDSSIDVE+RGAAISPSKLQVHNGSNGHNPHPHAAITTMG
Sbjct: 121 GFLMTMAADCVISYLYRKQNGDSSIDVELRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180

Query: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240
           SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN
Sbjct: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240

Query: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300
           RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL
Sbjct: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300

Query: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           SKGYT KDSV V NPNHKFVAVLLGIGIIAVVMIWDT
Sbjct: 301 SKGYTPKDSVGVVNPNHKFVAVLLGIGIIAVVMIWDT 337

[Close]

BLAST of Carg12867-RA vs. ExPASy TrEMBL
Match: A0A6J1IN23 (zinc transporter 11 OS=Cucurbita maxima OX=3661 GN=LOC111476718 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 3.7e-180
Identity = 326/337 (96.74%), Postives = 328/337 (97.33%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTF 60
           MAR LLL    LLLLSSAVAHSGHHDDDDAG+ EATPNLRSKPLILVKIGCLILIFFGTF
Sbjct: 1   MARHLLLSIFLLLLLSSAVAHSGHHDDDDAGDAEATPNLRSKPLILVKIGCLILIFFGTF 60

Query: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120
           IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV
Sbjct: 61  IPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACV 120

Query: 121 GFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMG 180
           GFLMTMAADCVISYLYRKQDGDSSIDVE+RG AISPSKLQVHNGSNGHNPHPHAAITTMG
Sbjct: 121 GFLMTMAADCVISYLYRKQDGDSSIDVELRGTAISPSKLQVHNGSNGHNPHPHAAITTMG 180

Query: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240
           SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN
Sbjct: 181 SFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPN 240

Query: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300
           RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL
Sbjct: 241 RPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLL 300

Query: 301 SKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           SKGYTAKDSV V NPNHKFVAVLLGIGIIAVVMIWDT
Sbjct: 301 SKGYTAKDSVGVVNPNHKFVAVLLGIGIIAVVMIWDT 337

[Close]

BLAST of Carg12867-RA vs. ExPASy TrEMBL
Match: A0A0A0K8F9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G162550 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.9e-160
Identity = 289/334 (86.53%), Postives = 308/334 (92.22%), Query Frame = 0


Query: 7   LLSIFLLLLSSAVAHSGHHDDDDAG---EVEATPNLRSKPLILVKIGCLILIFFGTFIPG 66
           +L    LLLSSA AHSGH DDDD+     +  +PNLRSKPLILVKI CLILIFFGTFIPG
Sbjct: 1   MLRFLFLLLSSAAAHSGHSDDDDSAAGDSLSPSPNLRSKPLILVKITCLILIFFGTFIPG 60

Query: 67  ISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACVGFL 126
           ISPCFFKWNDGFL+LGTQFAGGVFFGTAMMHFLSDANETF DLTD AYPFAFMLAC+GFL
Sbjct: 61  ISPCFFKWNDGFLLLGTQFAGGVFFGTAMMHFLSDANETFRDLTDNAYPFAFMLACLGFL 120

Query: 127 MTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMGSFG 186
           MTMAADCVISYLYRK   DSS DVE+RGAA SPSK QV NGSNGH+ HPH A+TTMGSFG
Sbjct: 121 MTMAADCVISYLYRKPTADSSTDVELRGAATSPSKFQVQNGSNGHHTHPHQALTTMGSFG 180

Query: 187 DSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPNRPL 246
           DSILLIVALCFHSVFEGIAIGVAET+ADAW+ALWTISLHKVFAAIAMGIALLRMIPNRPL
Sbjct: 181 DSILLIVALCFHSVFEGIAIGVAETKADAWKALWTISLHKVFAAIAMGIALLRMIPNRPL 240

Query: 247 LSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLLSKG 306
           LSSAAYSFAFAISSPIGIAIG+IIDATT+GAVADWIFAISMGLACGVFIYVSINHLLSKG
Sbjct: 241 LSSAAYSFAFAISSPIGIAIGIIIDATTQGAVADWIFAISMGLACGVFIYVSINHLLSKG 300

Query: 307 YTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           YT +DSV+VDNPN+KF+AVLLGIG+IA+VMIWDT
Sbjct: 301 YTPRDSVLVDNPNYKFLAVLLGIGVIAIVMIWDT 334

[Close]

BLAST of Carg12867-RA vs. ExPASy TrEMBL
Match: A0A1S3CI48 (zinc transporter 11 OS=Cucumis melo OX=3656 GN=LOC103501236 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.8e-159
Identity = 290/336 (86.31%), Postives = 307/336 (91.37%), Query Frame = 0


Query: 4   RLLLLSIFLLLLSSAVAHSGHHDDDDAGE--VEATPNLRSKPLILVKIGCLILIFFGTFI 63
           R L L + LLLLSSA AHSGH D+D A E     +PNLRSKPLILVKI CLILIFFGTFI
Sbjct: 3   RFLFLLLLLLLLSSAAAHSGHSDEDAAPEDGPSPSPNLRSKPLILVKIACLILIFFGTFI 62

Query: 64  PGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFMLACVG 123
           PGISPCFFKWNDGFL+LGTQFAGGVFFGTAMMHFLSDANETF DLTD  YPFAFMLA  G
Sbjct: 63  PGISPCFFKWNDGFLLLGTQFAGGVFFGTAMMHFLSDANETFRDLTDNDYPFAFMLASSG 122

Query: 124 FLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPHAAITTMGS 183
           FLMTMAADCVISYLYRK   DSS DVE+ GAA SPSK QV NGSNGH+PHPH A+TTMGS
Sbjct: 123 FLMTMAADCVISYLYRKPTADSSTDVELGGAATSPSKFQVQNGSNGHHPHPHQALTTMGS 182

Query: 184 FGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGIALLRMIPNR 243
           FGDSILLIVALCFHSVFEGIAIGVAETEADAW+ALWTISLHKVFAAIAMGIALLRMIPNR
Sbjct: 183 FGDSILLIVALCFHSVFEGIAIGVAETEADAWKALWTISLHKVFAAIAMGIALLRMIPNR 242

Query: 244 PLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFIYVSINHLLS 303
           PLLSSAAYSFAFAISSPIGIAIG++IDATT+GAVADWIFAISMGLACGVFIYVSINHLLS
Sbjct: 243 PLLSSAAYSFAFAISSPIGIAIGIVIDATTQGAVADWIFAISMGLACGVFIYVSINHLLS 302

Query: 304 KGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           KGYT +DSV+VDNPN+KF+AVLLGIG+IA+VMIWDT
Sbjct: 303 KGYTPRDSVVVDNPNYKFLAVLLGIGVIAIVMIWDT 338

[Close]

BLAST of Carg12867-RA vs. ExPASy TrEMBL
Match: A0A6J1CD53 (zinc transporter 11 OS=Momordica charantia OX=3673 GN=LOC111010579 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.4e-150
Identity = 278/345 (80.58%), Postives = 303/345 (87.83%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAHSGHHDDDDAG---EVEATPNLRSKPLILVKIGCLILIFF 60
           MAR LLL S+  LLLSSA AH G HDD D       +  PNLRSKPL+LVKIGCLILIF 
Sbjct: 1   MARSLLLFSLLFLLLSSAAAHGGSHDDGDGDAPTAADQAPNLRSKPLLLVKIGCLILIFV 60

Query: 61  GTFIPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAFML 120
           GTFIPGISP FFKWNDGFLVLGTQFAGGVF GTAMMHFLSDANETFGDLTD+ YPFAFML
Sbjct: 61  GTFIPGISPYFFKWNDGFLVLGTQFAGGVFLGTAMMHFLSDANETFGDLTDRQYPFAFML 120

Query: 121 ACVGFLMTMAADCVISYLYRKQDGDSSI-----DVEIRGAAISPSKLQVHNGSNGHNPHP 180
           AC GFLMTMAADCVIS+LYRKQ GDSSI     D+E+RGAA SP K Q  NGS+ H+P P
Sbjct: 121 ACAGFLMTMAADCVISHLYRKQTGDSSIGGYGGDLELRGAATSPPKFQGQNGSSSHHPSP 180

Query: 181 HAAITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGI 240
             A+T+MGSFGDSILLIVALCFHS+FEGIAIGVAETEADAWRALWTISLHKVFAAIAMGI
Sbjct: 181 --ALTSMGSFGDSILLIVALCFHSIFEGIAIGVAETEADAWRALWTISLHKVFAAIAMGI 240

Query: 241 ALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGVFI 300
           ALLRMIP+RP LS+ AY+FAFAISSPIG+ IG+IIDATT+GAVADWIFAISMGLACGVFI
Sbjct: 241 ALLRMIPDRPFLSTVAYAFAFAISSPIGVGIGIIIDATTQGAVADWIFAISMGLACGVFI 300

Query: 301 YVSINHLLSKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           YVS+NHLLSKGYT +++V+VDN N+KFVAVLLGIG+IAVVMIWDT
Sbjct: 301 YVSVNHLLSKGYTPREAVLVDNQNYKFVAVLLGIGVIAVVMIWDT 343

[Close]

BLAST of Carg12867-RA vs. TAIR 10
Match: AT1G55910.1 (zinc transporter 11 precursor )

HSP 1 Score: 392.1 bits (1006), Expect = 4.5e-109
Identity = 205/347 (59.08%), Postives = 255/347 (73.49%), Query Frame = 0


Query: 1   MARRLLLLSIFLLLLSSAVAH--SGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFG 60
           M+R L+   +FL+L+   ++H   G HDDD+A  V+++ +L+SK LI VKI CL++IF  
Sbjct: 1   MSRSLVFFFLFLVLVVPCLSHGTGGDHDDDEASHVKSS-DLKSKSLISVKIACLVIIFVL 60

Query: 61  TFIPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDL--------TDKA 120
           TFI G+SP F KW+ GFLVLGTQFAGGVF  TA+MHFLSDA+ETF  L           A
Sbjct: 61  TFISGVSPYFLKWSQGFLVLGTQFAGGVFLATALMHFLSDADETFRGLLTAEGESEPSPA 120

Query: 121 YPFAFMLACVGFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNP 180
           YPFA+MLAC GF++TM AD VI+++Y K   D  +                     G + 
Sbjct: 121 YPFAYMLACAGFMLTMLADSVIAHIYSKTQNDLEL--------------------QGEDK 180

Query: 181 HPHAAITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAAIAM 240
               + TT  S GDSILLIVALCFHSVFEGIAIG++ET++DAWRALWTI+LHK+FAAIAM
Sbjct: 181 SNQRSATTETSIGDSILLIVALCFHSVFEGIAIGISETKSDAWRALWTITLHKIFAAIAM 240

Query: 241 GIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLACGV 300
           GIALLRMIP+RPL SS  YSFAFAISSPIG+AIG++IDATT+G++ADWIFA+SM LACGV
Sbjct: 241 GIALLRMIPDRPLFSSITYSFAFAISSPIGVAIGIVIDATTQGSIADWIFALSMSLACGV 300

Query: 301 FIYVSINHLLSKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWDT 338
           F+YVS+NHLL+KGY     V VD P +KF+AVL G+ +IA+VMIWDT
Sbjct: 301 FVYVSVNHLLAKGYRPNKKVHVDEPRYKFLAVLFGVVVIAIVMIWDT 326

[Close]

BLAST of Carg12867-RA vs. TAIR 10
Match: AT5G59520.1 (ZRT/IRT-like protein 2 )

HSP 1 Score: 349.0 bits (894), Expect = 4.3e-96
Identity = 181/349 (51.86%), Postives = 242/349 (69.34%), Query Frame = 0


Query: 5   LLLLSIFLLLLSSAVAHSGHHDDDDAGEVEATP---------NLRSKPLILVKIGCLILI 64
           L  LSI  L  S  +AH G  D D+  E    P         NLRSK L+LVKI C+I++
Sbjct: 12  LFFLSIIFLCFSLILAHGGIDDGDEEEETNQPPPATGTTTVVNLRSKSLVLVKIYCIIIL 71

Query: 65  FFGTFIPGISPCFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGDLTDKAYPFAF 124
           FF TF+ G+SP F++WN+ FL+LGTQF+GG+F  TA++HFLSDANETF  L  K YP+AF
Sbjct: 72  FFSTFLAGVSPYFYRWNESFLLLGTQFSGGIFLATALIHFLSDANETFRGLKHKEYPYAF 131

Query: 125 MLACVGFLMTMAADCVISYLYRKQD--------GDSSIDVEIRGAAISPSKLQVHNGSNG 184
           MLA  G+ +TM AD  ++++    +        G+S  D ++  A     + ++ +G + 
Sbjct: 132 MLAAAGYCLTMLADVAVAFVAAGSNNNHVGASVGESREDDDV--AVKEEGRREIKSGVD- 191

Query: 185 HNPHPHAAITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFAA 244
                 A I T G FGD+ LLI ALCFHS+FEGIAIG+++T++DAWR LWTISLHKVFAA
Sbjct: 192 ---VSQALIRTSG-FGDTALLIFALCFHSIFEGIAIGLSDTKSDAWRNLWTISLHKVFAA 251

Query: 245 IAMGIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGLA 304
           +AMGIALL++IP RP   +  YSFAF ISSPIG+ IG+ I+AT++GA  DW +AISMGLA
Sbjct: 252 VAMGIALLKLIPKRPFFLTVVYSFAFGISSPIGVGIGIGINATSQGAGGDWTYAISMGLA 311

Query: 305 CGVFIYVSINHLLSKGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIWD 337
           CGVF+YV++NHL+SKGY  ++    D P +KF+AV LG+ +++VVMIWD
Sbjct: 312 CGVFVYVAVNHLISKGYKPREECYFDKPIYKFIAVFLGVALLSVVMIWD 353

[Close]

BLAST of Carg12867-RA vs. TAIR 10
Match: AT4G19680.2 (iron regulated transporter 2 )

HSP 1 Score: 75.5 bits (184), Expect = 9.2e-14
Identity = 82/354 (23.16%), Postives = 155/354 (43.79%), Query Frame = 0


Query: 6   LLLSIFLLLLSSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTFIPGIS 65
           +LL +F   +S A++ +  H D         P +     + +KI  ++ I   + I   S
Sbjct: 9   ILLILFTFTVSPAISTAPEHCDSGFD----NPCINKAKALPLKIVAIVAILTTSLIGVTS 68

Query: 66  PCFFKW-------NDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGD--LTDK---AYPF 125
           P F ++        +GF+++   F+ G+  GT  MH L D+ E      L+D     +PF
Sbjct: 69  PLFSRYISFLRPDGNGFMIVKC-FSSGIILGTGFMHVLPDSFEMLSSKCLSDNPWHKFPF 128

Query: 126 AFMLACVGFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNPHPH 185
           A  +A +  L+T+A D + + LY  ++    +  E  G      +  +H   + H+ H H
Sbjct: 129 AGFVAMMSGLVTLAIDSITTSLYTGKNSVGPVPDEEYGI---DQEKAIHMVGHNHS-HGH 188

Query: 186 AAITTMGSFGD-------SILLIVALCFHSVFEGIAIGVAETEADAWRALWTISLHKVFA 245
             +      G        +++L V + FHSV  G+++G           +  +  H +F 
Sbjct: 189 GVVLATKDDGQLLRYQVIAMVLEVGILFHSVVIGLSLGATNDSCTIKGLIIALCFHHLFE 248

Query: 246 AIAMGIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIFAISMGL 305
            I +G  +L+   +   +     +F F  ++P GI +G+ + +         +  I +  
Sbjct: 249 GIGLGGCILQ--ADFTNVKKFLMAFFFTGTTPCGIFLGIALSSIYRDNSPTALITIGLLN 308

Query: 306 AC--GVFIYVSINHLLS---KGYTAKDSVMVDNPNHKFVAVLLGIGIIAVVMIW 336
           AC  G+ IY+++  LL+    G   + S+ +      F A LLG  +++VV +W
Sbjct: 309 ACSAGMLIYMALVDLLATEFMGSMLQGSIKLQ--IKCFTAALLGCAVMSVVAVW 349

[Close]

BLAST of Carg12867-RA vs. TAIR 10
Match: AT4G19690.2 (iron-regulated transporter 1 )

HSP 1 Score: 69.7 bits (169), Expect = 5.1e-12
Identity = 77/358 (21.51%), Postives = 150/358 (41.90%), Query Frame = 0


Query: 5   LLLLSIFLLLL--SSAVAHSGHHDDDDAGEVEATPNLRSKPLILVKIGCLILIFFGTFIP 64
           LL+ +IFL+L+  S A++ +     ++ G   A P +     + +K+  + +I   + I 
Sbjct: 7   LLMKTIFLVLIFVSFAISPATSTAPEECGSESANPCVNKAKALPLKVIAIFVILIASMI- 66

Query: 65  GISPCFFKWNDGFL-------VLGTQFAGGVFFGTAMMHFLSDANETFGDLTDK-----A 124
           G+    F  N  FL        +   FA G+  GT  MH L D+ E    +  +      
Sbjct: 67  GVGAPLFSRNVSFLQPDGNIFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHK 126

Query: 125 YPFAFMLACVGFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHNP 184
           +PF+  LA +  L+T+A D + + LY  ++             I P     H   +GH P
Sbjct: 127 FPFSGFLAMLSGLITLAIDSMATSLYTSKN----------AVGIMP-----HGHGHGHGP 186

Query: 185 HPHAAITTMGSFGD----------SILLIVALCFHSVFEGIAIGVAETEADAWRALWTIS 244
                +                  +++L + +  HSV  G+++G           +  + 
Sbjct: 187 ANDVTLPIKEDDSSNAQLLRYRVIAMVLELGIIVHSVVIGLSLGATSDTCTIKGLIAALC 246

Query: 245 LHKVFAAIAMGIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGLIIDATTEGAVADWIF 304
            H++F  + +G  +L+       +     +F FA+++P GIA+G+ +    +      + 
Sbjct: 247 FHQMFEGMGLGGCILQ--AEYTNMKKFVMAFFFAVTTPFGIALGIALSTVYQDNSPKALI 306

Query: 305 AISMGLAC--GVFIYVSINHLLSKGYTA-KDSVMVDNPNHKFVAVLLGIGIIAVVMIW 336
            + +  AC  G+ IY+++  LL+  +   K    +       +A LLG G ++++  W
Sbjct: 307 TVGLLNACSAGLLIYMALVDLLAAEFMGPKLQGSIKMQFKCLIAALLGCGGMSIIAKW 346

[Close]

BLAST of Carg12867-RA vs. TAIR 10
Match: AT2G32270.1 (zinc transporter 3 precursor )

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-11
Identity = 88/352 (25.00%), Postives = 158/352 (44.89%), Query Frame = 0


Query: 9   SIFLLLLSSAVAHSGH----------HDDDDAGEVEATP-NLRSKPLILVK--IGCLILI 68
           S+ LLL++   A  GH          H+DD   +  A    + + P +L+   IG L  +
Sbjct: 14  SVSLLLIAVVNAAEGHSHGGPKCECSHEDDHENKAGARKYKIAAIPTVLIAGIIGVLFPL 73

Query: 69  FFGTFIPGISP--CFFKWNDGFLVLGTQFAGGVFFGTAMMHFLSDANETFGD--LTDKA- 128
             G   P + P  CFF        +   FA GV   T  MH L +A E      LT +A 
Sbjct: 74  -LGKVFPSLRPETCFF-------FVTKAFAAGVILATGFMHVLPEAYEMLNSPCLTSEAW 133

Query: 129 -YPFAFMLACVGFLMTMAADCVISYLYRKQDGDSSIDVEIRGAAISPSKLQVHNGSNGHN 188
            +PF   +A +  ++T++ D   +  + K    +S               +V +G  G +
Sbjct: 134 EFPFTGFIAMIAAILTLSVDTFATSSFYKSHCKAS--------------KRVSDGETGES 193

Query: 189 PHPHAAITTMGSFGDSILLIVALCFHSVFEGIAIGVAETEADAWRALW-TISLHKVFAAI 248
                 +  + +   + +L + +  HSV  GI++G +++  DA +AL+  +  H+ F  +
Sbjct: 194 SVDSEKVQILRTRVIAQVLELGIIVHSVVIGISLGASQS-PDAAKALFIALMFHQCFEGL 253

Query: 249 AMGIALLRMIPNRPLLSSAAYSFAFAISSPIGIAIGL-IIDATTEGA-VADWIFAISMGL 308
            +G  + +       LS    S  FAI++PIGI +G+ I ++  E +  A  +  +    
Sbjct: 254 GLGGCIAQ--GKFKCLSVTIMSTFFAITTPIGIVVGMGIANSYDESSPTALIVQGVLNAA 313

Query: 309 ACGVFIYVSINHLLSKGYTAKDSVMVDNPNHKF---VAVLLGIGIIAVVMIW 336
           + G+ IY+S+  LL+  +T     M  N   +    +A+LLG G+++++  W
Sbjct: 314 SAGILIYMSLVDLLAADFT--HPKMQSNTGLQIMAHIALLLGAGLMSLLAKW 338

The following BLAST results are available for this feature:

BLAST of Carg12867-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024965.1	1.9e-186	100.00	Zinc transporter 11 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6592088.1	9.4e-186	99.41	Zinc transporter 11, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023535779.1	6.1e-185	99.11	zinc transporter 11 [Cucurbita pepo subsp. pepo]	[more]
XP_022937302.1	1.7e-182	98.22	zinc transporter 11 [Cucurbita moschata]	[more]
XP_022976274.1	7.7e-180	96.74	zinc transporter 11 [Cucurbita maxima]	[more]

BLAST of Carg12867-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94EG9	6.3e-108	59.08	Zinc transporter 11 OS=Arabidopsis thaliana OX=3702 GN=ZIP11 PE=2 SV=1	[more]
Q852F6	1.2e-103	59.69	Zinc transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP2 PE=2 SV=1	[more]
Q9LTH9	6.1e-95	51.86	Zinc transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ZIP2 PE=2 SV=1	[more]
Q94DG6	1.1e-91	52.34	Zinc transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP1 PE=1 SV=1	[more]
P59889	8.1e-15	27.01	Zinc transporter ZIP1 OS=Danio rerio OX=7955 GN=slc39a1 PE=2 SV=1	[more]

BLAST of Carg12867-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FAT9	8.0e-183	98.22	zinc transporter 11 OS=Cucurbita moschata OX=3662 GN=LOC111443628 PE=4 SV=1	[more]
A0A6J1IN23	3.7e-180	96.74	zinc transporter 11 OS=Cucurbita maxima OX=3661 GN=LOC111476718 PE=4 SV=1	[more]
A0A0A0K8F9	1.9e-160	86.53	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G162550 PE=4 SV=1	[more]
A0A1S3CI48	4.8e-159	86.31	zinc transporter 11 OS=Cucumis melo OX=3656 GN=LOC103501236 PE=4 SV=1	[more]
A0A6J1CD53	2.4e-150	80.58	zinc transporter 11 OS=Momordica charantia OX=3673 GN=LOC111010579 PE=4 SV=1	[more]

BLAST of Carg12867-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55910.1	4.5e-109	59.08	zinc transporter 11 precursor	[more]
AT5G59520.1	4.3e-96	51.86	ZRT/IRT-like protein 2	[more]
AT4G19680.2	9.2e-14	23.16	iron regulated transporter 2	[more]
AT4G19690.2	5.1e-12	21.51	iron-regulated transporter 1	[more]
AT2G32270.1	1.5e-11	25.00	zinc transporter 3 precursor	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003689	Zinc/iron permease	PFAM	PF02535	Zip	coord: 46..332 e-value: 6.1E-46 score: 157.2
None	No IPR available	PANTHER	PTHR11040	ZINC/IRON TRANSPORTER	coord: 23..336
None	No IPR available	PANTHER	PTHR11040:SF140	ZINC TRANSPORTER 11	coord: 23..336

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12867	Carg12867	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12867-RA:1	Carg12867-RA:1	exon
Carg12867-RA:2	Carg12867-RA:2	exon
Carg12867-RA:3	Carg12867-RA:3	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12867-RA:three_prime_utr	Carg12867-RA:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12867-RA:cds	Carg12867-RA:cds_3	CDS
Carg12867-RA:cds	Carg12867-RA:cds_2	CDS
Carg12867-RA:cds	Carg12867-RA:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12867-RA:five_prime_utr	Carg12867-RA:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12867-RA	Carg12867-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030001	metal ion transport
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0071577	zinc ion transmembrane transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046873	metal ion transmembrane transporter activity
molecular_function	GO:0005385	zinc ion transmembrane transporter activity