Carg12753-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12753-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein of unknown function (DUF1218)
Location	Carg_Chr09: 4831483 .. 4834313 (-)
Sequence length	1077
RNA-Seq Expression	Carg12753-RA
Synteny	Carg12753-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTTGTGCAAGCTACTGGCAAGATGTTGAGACGTTATTTTCTGTTTCTAATTTTTTTTCTTTTTCACTCTGTTTTCATCAGAGCAAAGCTTGTTTCTCTGTTCCTCTTATAAACCCCAACAAGAAGAGACGCAGAGAGACAGTGAAACATTACTGAATCTGCTCAAGAATTGAGTAGAAACAAATATCAGGGCAGATCAAAAAAGCTCTAAACCAGTGTATTTTGTCCATCAATGGCGTCCAAATTGGTTCTGATTATAGTTATTGTACTCGATGTTATTGCTTTTGGCCTGGCCGTTGCCGCTGAGCAAAGAAGAAGCACTGTAAGCTTCAGTCCCTCTCTCCATCTCTTCCATTCATTGTCGCTTTCTTTTGTGCTTTTTACTGCAATTTTTGTCGGTTTGGGGTTCTTTGATTTCTGGGTCTGTTTGGTTTCTGTGATTCTGTTTGTGGGTCGTTTTGGAGTTGATGAAATCGGCATTGTGAAATCGAGAATGGATGAACCAAGAATCTTTCCTTTTCTGGGTTGTTCTTGGTCACCAAACAACGATTTATTGGCTTTTCTGCTTCAAATTTGTGGTATCAGTTCATCCATTAGGGTTCATAAATTAGGGCTTTAGTTGTTAAAAAGCAGGAAAATGGATAATGGATTAAGTTGTTCTTGGTTTGAAAATTGTTGACATGTTCATGATTTAGAGCATACTAACAATAGTGACTATACTTGGCTTTTCTAAAATGCAATTCTGAGCTGTTCTGAAACATTGTCCATTTTGAGTCTAAGCTCTCGTGGAGATGTATTCCTTACTTATAAACCCATGATCAACCCCTTAATTAACCGATGCGGGACTCCTCTCCTGACAATCCTCCCCTCGAACAAAGCACATCATAGAGCCTTCCCTGAGGCCTATGGAGCCCTCGAACAGCCTCCCCTGAATCGAAGCTCGACTCCTTCTCTGGAGCTCTTGAAAAAGTACACCATTTGCTCGATACTTGAGTCACTTTTGAACTTCGAGGCTCACAACTTCTTTGTTCGATATTTGAGGATTCTATTGACATGACTAAGTTAAGGGTATGACTCTAATACCATGTTAGGAATCATGACTCTCCACAATGGTATGATATTGTCCATTTTGAGCCTAAGCTCTTATGGCTTTACTTTTGGTTTCCCTAAAAGGTCTCATACCAATGTAGATGTATTTCTTACTTATAAAACCCATGATCAACTCCGTAATTAGCCGATGTGGGATTCCTCTCCCAACAATCCTCCTCTCGAACAAAGCACACCATAGAGCCTCCCCTGATGCCTATGGAGCCCTCGAACAGTCTCCCCTTAATCGAGGCTCGACTCCTTTTCTATAGTCCTCGAACAAAGTACACTCTTTGCTCGACACTTGAGTCACTTTTGACTACACCTTCGAGGTTCACAACTTCTTTGTTCGTTATTTGAGGATTCCATTGACATGCCTAAGTTAAGGGTATAACTCTGATACCATGTTAGGAATTACGAGTCTCCACAATGATATGATATTGTCTACTTTGAGCATAAGCTCTTATGGCTTTGCTTTTGATTTCCTCAAAAGGCCTCATACCAATGTAGATGTATTTCTGACTTATAAACCCACGATCAACTCCTTAATTAGCCGATGTGGAACAAAGGAGACTTCCGTATATTTCTTTGTTTCTAACTACTAAGCACGTTACTTCAAAATGGTCGGGCGTTTACTTTCGATCTAGACAATGGTAAAATTAAGGTAACTGTTCTCATGTGTTTCCACCATCACTCACTCGTTCATTCATATTGCTATGAATCTAATCTTACTAATAAGTTGTATCCAACGCATACTCAATAACTGGTTTCGTTCTGTACTCCTTTGGTTTCTTCGTTACTTCGAAGATCGACGAGTTGGTTTCAGCTGATTTCTAATGTCTATATGATATACTAGCATAGGATTTGATGCATTTCCGCTCATAATGTATCGTGTATTTATCGTTTGATAACAGGCCAACGTTGTACCAGATAGTGAAAAGAATTACAACTATTGTGTGTATGACTCCGACATCTCGACAGGCTATGGCGTCGGTGCTTTTCTACTTCTCATGGCTAGCCAGGCCCTCATAATGGTAGCTAGCCGATGTTTTTGTTGCGGGAAGTCGTTAAGTCCTGGAGGTTCGAGGGCATGGGCTATTATCCTATTCATAATCTGCTGGTAAGTGAACCTATTTGTGAGCTTCACTCCATAAGTTCAAACTAAAGGGTCCAATCCAATACACACAATCTTGCAGGGTGTTTTTCTTCATTGCTGAGGTTTGCTTGTTCGCGGGCTCGATAAGGAACGCCTACCACACCAAGTACCGAACCATGTTTGGTGACAATCCTCCGTCGTGCCAAACTTTGAGGAAGGGAGTTTTCGGTGCAGGTGCAGCCTTCATTTTCCTTAATGCCATTGTATCAGAACTCTACTATGTGTTCTACTCGAAGGCGAGGGAAAGCTTCCTTCCCTACGGCGGAGAAACGGGCGTTGGCATGGGAACCTACAAATGAGTGTACGTATAATCAAATCTAATAGGATTGATGCTCCCGGTTCGATGTCGAGATAGTTTTGCATATCAACTACTGGTTTTAGTTTAGGAAGAATTATCAGTATCAGAATTTTTAGTCTGTGAAGTTCTTTGTTTTCTCTCCATTTTAACCATTGCTGCTGCGCTTTTCTTGTCTTTTTGGTGGATTGTTACATTTGTATTGACAATAAGAGAAACATTGGTTGTCTTCCTATAAATATTTGTATCTTTTGTGAGACCTCAGTGGCAATGAATTCATATACTCTTCGGCT

mRNA sequence

GATTTGTGCAAGCTACTGGCAAGATGTTGAGACGTTATTTTCTGTTTCTAATTTTTTTTCTTTTTCACTCTGTTTTCATCAGAGCAAAGCTTGTTTCTCTGTTCCTCTTATAAACCCCAACAAGAAGAGACGCAGAGAGACAGTGAAACATTACTGAATCTGCTCAAGAATTGAGTAGAAACAAATATCAGGGCAGATCAAAAAAGCTCTAAACCAGTGTATTTTGTCCATCAATGGCGTCCAAATTGGTTCTGATTATAGTTATTGTACTCGATGTTATTGCTTTTGGCCTGGCCGTTGCCGCTGAGCAAAGAAGAAGCACTGCCAACGTTGTACCAGATAGTGAAAAGAATTACAACTATTGTGTGTATGACTCCGACATCTCGACAGGCTATGGCGTCGGTGCTTTTCTACTTCTCATGGCTAGCCAGGCCCTCATAATGGTAGCTAGCCGATGTTTTTGTTGCGGGAAGTCGTTAAGTCCTGGAGGTTCGAGGGCATGGGCTATTATCCTATTCATAATCTGCTGGGTGTTTTTCTTCATTGCTGAGGTTTGCTTGTTCGCGGGCTCGATAAGGAACGCCTACCACACCAAGTACCGAACCATGTTTGGTGACAATCCTCCGTCGTGCCAAACTTTGAGGAAGGGAGTTTTCGGTGCAGGTGCAGCCTTCATTTTCCTTAATGCCATTGTATCAGAACTCTACTATGTGTTCTACTCGAAGGCGAGGGAAAGCTTCCTTCCCTACGGCGGAGAAACGGGCGTTGGCATGGGAACCTACAAATGAGTGTACGTATAATCAAATCTAATAGGATTGATGCTCCCGGTTCGATGTCGAGATAGTTTTGCATATCAACTACTGGTTTTAGTTTAGGAAGAATTATCAGTATCAGAATTTTTAGTCTGTGAAGTTCTTTGTTTTCTCTCCATTTTAACCATTGCTGCTGCGCTTTTCTTGTCTTTTTGGTGGATTGTTACATTTGTATTGACAATAAGAGAAACATTGGTTGTCTTCCTATAAATATTTGTATCTTTTGTGAGACCTCAGTGGCAATGAATTCATATACTCTTCGGCT

Coding sequence (CDS)

ATGGCGTCCAAATTGGTTCTGATTATAGTTATTGTACTCGATGTTATTGCTTTTGGCCTGGCCGTTGCCGCTGAGCAAAGAAGAAGCACTGCCAACGTTGTACCAGATAGTGAAAAGAATTACAACTATTGTGTGTATGACTCCGACATCTCGACAGGCTATGGCGTCGGTGCTTTTCTACTTCTCATGGCTAGCCAGGCCCTCATAATGGTAGCTAGCCGATGTTTTTGTTGCGGGAAGTCGTTAAGTCCTGGAGGTTCGAGGGCATGGGCTATTATCCTATTCATAATCTGCTGGGTGTTTTTCTTCATTGCTGAGGTTTGCTTGTTCGCGGGCTCGATAAGGAACGCCTACCACACCAAGTACCGAACCATGTTTGGTGACAATCCTCCGTCGTGCCAAACTTTGAGGAAGGGAGTTTTCGGTGCAGGTGCAGCCTTCATTTTCCTTAATGCCATTGTATCAGAACTCTACTATGTGTTCTACTCGAAGGCGAGGGAAAGCTTCCTTCCCTACGGCGGAGAAACGGGCGTTGGCATGGGAACCTACAAATGA

Protein sequence

MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFLLLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHTKYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGMGTYK

Homology

[Close]

BLAST of Carg12753-RA vs. NCBI nr
Match: XP_022935762.1 (uncharacterized protein LOC111442584 [Cucurbita moschata] >XP_023535137.1 uncharacterized protein LOC111796652 [Cucurbita pepo subsp. pepo] >KAG6591977.1 hypothetical protein SDJN03_14323, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024853.1 hypothetical protein SDJN02_13672 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 367.5 bits (942), Expect = 6.9e-98
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL
Sbjct: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT
Sbjct: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. NCBI nr
Match: XP_022976585.1 (uncharacterized protein LOC111476938 [Cucurbita maxima] >XP_022976586.1 uncharacterized protein LOC111476938 [Cucurbita maxima])

HSP 1 Score: 366.7 bits (940), Expect = 1.2e-97
Identity = 182/184 (98.91%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSD+STGYGVGAFL
Sbjct: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDVSTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. NCBI nr
Match: XP_038900268.1 (uncharacterized protein LOC120087350 [Benincasa hispida])

HSP 1 Score: 356.3 bits (913), Expect = 1.6e-94
Identity = 176/184 (95.65%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKL+LIIV VLDVIAFGLAVAAEQRRSTANVV D EK YNYCVYDSDISTGYGVGAFL
Sbjct: 1   MASKLILIIVFVLDVIAFGLAVAAEQRRSTANVVQDKEKEYNYCVYDSDISTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQ LIMVASRCFCCGKSLSPGGSRAWA+ILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQTLIMVASRCFCCGKSLSPGGSRAWAVILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. NCBI nr
Match: XP_031740219.1 (uncharacterized protein LOC101208795 [Cucumis sativus] >XP_031740220.1 uncharacterized protein LOC101208795 [Cucumis sativus] >KGN55148.1 hypothetical protein Csa_012625 [Cucumis sativus])

HSP 1 Score: 354.4 bits (908), Expect = 6.0e-94
Identity = 174/184 (94.57%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKL+LIIV VLD+IAFGLAVAAEQRRSTANVV D EK YNYCVYDSDISTGYGVGAFL
Sbjct: 1   MASKLILIIVFVLDIIAFGLAVAAEQRRSTANVVQDKEKEYNYCVYDSDISTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQ LIM ASRCFCCGKSLSPGGSRAWA+ILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQTLIMAASRCFCCGKSLSPGGSRAWAVILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. NCBI nr
Match: XP_008452482.1 (PREDICTED: uncharacterized protein LOC103493502 [Cucumis melo] >KAA0064347.1 DUF1218 domain-containing protein [Cucumis melo var. makuwa] >TYK20240.1 DUF1218 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 347.8 bits (891), Expect = 5.6e-92
Identity = 172/184 (93.48%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKL+LIIV VLDVIAFGLAVAAEQRRSTANVV D EK+YNYCVYDSD+STGYGVGAFL
Sbjct: 1   MASKLILIIVFVLDVIAFGLAVAAEQRRSTANVVQDKEKDYNYCVYDSDVSTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQ LIM ASRCFCCGKSLSPGGSRA A+ILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQTLIMAASRCFCCGKSLSPGGSRASAVILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSC+TLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCRTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. ExPASy TrEMBL
Match: A0A6J1F6H9 (uncharacterized protein LOC111442584 OS=Cucurbita moschata OX=3662 GN=LOC111442584 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.3e-98
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL
Sbjct: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT
Sbjct: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. ExPASy TrEMBL
Match: A0A6J1IJW2 (uncharacterized protein LOC111476938 OS=Cucurbita maxima OX=3661 GN=LOC111476938 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 5.7e-98
Identity = 182/184 (98.91%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSD+STGYGVGAFL
Sbjct: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDVSTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. ExPASy TrEMBL
Match: A0A0A0L071 (Fiber protein Fb34 OS=Cucumis sativus OX=3659 GN=Csa_4G638370 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.9e-94
Identity = 174/184 (94.57%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKL+LIIV VLD+IAFGLAVAAEQRRSTANVV D EK YNYCVYDSDISTGYGVGAFL
Sbjct: 1   MASKLILIIVFVLDIIAFGLAVAAEQRRSTANVVQDKEKEYNYCVYDSDISTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQ LIM ASRCFCCGKSLSPGGSRAWA+ILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQTLIMAASRCFCCGKSLSPGGSRAWAVILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. ExPASy TrEMBL
Match: A0A5A7VBE4 (DUF1218 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003790 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.7e-92
Identity = 172/184 (93.48%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKL+LIIV VLDVIAFGLAVAAEQRRSTANVV D EK+YNYCVYDSD+STGYGVGAFL
Sbjct: 1   MASKLILIIVFVLDVIAFGLAVAAEQRRSTANVVQDKEKDYNYCVYDSDVSTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQ LIM ASRCFCCGKSLSPGGSRA A+ILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQTLIMAASRCFCCGKSLSPGGSRASAVILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSC+TLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCRTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. ExPASy TrEMBL
Match: A0A1S3BTB6 (uncharacterized protein LOC103493502 OS=Cucumis melo OX=3656 GN=LOC103493502 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.7e-92
Identity = 172/184 (93.48%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKL+LIIV VLDVIAFGLAVAAEQRRSTANVV D EK+YNYCVYDSD+STGYGVGAFL
Sbjct: 1   MASKLILIIVFVLDVIAFGLAVAAEQRRSTANVVQDKEKDYNYCVYDSDVSTGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LLMASQ LIM ASRCFCCGKSLSPGGSRA A+ILFIICWVFFFIAEVCLFAGS+RNAYHT
Sbjct: 61  LLMASQTLIMAASRCFCCGKSLSPGGSRASAVILFIICWVFFFIAEVCLFAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180
           KYRTMFGDNPPSC+TLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM
Sbjct: 121 KYRTMFGDNPPSCRTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFLPYGGETGVGM 180

Query: 181 GTYK 185
           GTYK
Sbjct: 181 GTYK 184

[Close]

BLAST of Carg12753-RA vs. TAIR 10
Match: AT4G27435.1 (Protein of unknown function (DUF1218) )

HSP 1 Score: 253.1 bits (645), Expect = 1.8e-67
Identity = 126/173 (72.83%), Postives = 142/173 (82.08%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASK+V  IV V ++IAFGLAVAAEQRRSTA VV D+E  YNYCVYDSD +TGYGVGAFL
Sbjct: 1   MASKIVSAIVFVFNLIAFGLAVAAEQRRSTARVVQDTEVQYNYCVYDSDRATGYGVGAFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
             +ASQ LIM+ SRCFCCGK L PGGSRA A+ILFI+ W+FF IAE+CL AGS+ NAYHT
Sbjct: 61  FSVASQILIMLVSRCFCCGKPLKPGGSRALALILFIVSWMFFLIAEICLLAGSVENAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESFL-PY 173
           KYRTMF DNPP CQTLRKGVF AGA+F+F NAIVS+ YY FY  A E+ L PY
Sbjct: 121 KYRTMFMDNPPDCQTLRKGVFAAGASFVFFNAIVSQFYYFFYFSAAEASLSPY 173

[Close]

BLAST of Carg12753-RA vs. TAIR 10
Match: AT1G52910.1 (Protein of unknown function (DUF1218) )

HSP 1 Score: 238.0 bits (606), Expect = 5.9e-63
Identity = 115/170 (67.65%), Postives = 137/170 (80.59%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKLV+IIV +LD+IA GLA+AAEQRRS   VVPD EK + +C Y SDI+T YG GAF+
Sbjct: 1   MASKLVIIIVFILDLIAVGLAIAAEQRRSVGKVVPDGEKEFEHCEYGSDIATSYGAGAFV 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LL  SQ +IMVASRCFCCGK+L PGGSRA  I+LF+ICWVFF IAEVCL AGSIRNAYHT
Sbjct: 61  LLFISQVIIMVASRCFCCGKALKPGGSRACGIMLFLICWVFFLIAEVCLLAGSIRNAYHT 120

Query: 121 KYRTMFG-DNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESF 170
            YR M+  +NPPSC+ +RKGVF AGA+F    AIVS+ YY+ YS+AR+ +
Sbjct: 121 TYRRMWNIENPPSCEVIRKGVFAAGASFALFTAIVSQFYYISYSRARDGY 170

[Close]

BLAST of Carg12753-RA vs. TAIR 10
Match: AT3G15480.1 (Protein of unknown function (DUF1218) )

HSP 1 Score: 236.1 bits (601), Expect = 2.2e-62
Identity = 116/170 (68.24%), Postives = 139/170 (81.76%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MASKLV+IIV +LD+IA GLA+AAEQRRS   V  D +K Y+YCVY +DI+T YG GAF+
Sbjct: 1   MASKLVVIIVFILDLIAVGLAIAAEQRRSVGKVETDRDKQYDYCVYGTDIATSYGAGAFV 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           LL  SQ LIM ASRCFCCGKSL+PGGSRA AIILF+ICWVFF IAE+CL A SIRNAYHT
Sbjct: 61  LLFVSQVLIMAASRCFCCGKSLNPGGSRACAIILFLICWVFFLIAEMCLLAASIRNAYHT 120

Query: 121 KYRTMFG-DNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARESF 170
           +YR M+  ++PPSC+ +RKGVF AGAAF    AIVS+ YYV YS+AR+++
Sbjct: 121 QYRKMWKVEDPPSCEVIRKGVFAAGAAFTLFTAIVSQFYYVCYSRARDAY 170

[Close]

BLAST of Carg12753-RA vs. TAIR 10
Match: AT1G61065.1 (Protein of unknown function (DUF1218) )

HSP 1 Score: 230.7 bits (587), Expect = 9.4e-61
Identity = 114/167 (68.26%), Postives = 140/167 (83.83%), Query Frame = 0


Query: 1   MASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFL 60
           MAS L+L++V V D+IAFGLAVAAEQRR+T  +  +S ++ +YCVYD DI+TG GVG+FL
Sbjct: 1   MASVLLLLLVFVFDLIAFGLAVAAEQRRTTWQISRES-RDLSYCVYDKDIATGLGVGSFL 60

Query: 61  LLMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHT 120
           +L+ASQ LIMVASRC CCG++L+P GSR+WAI LFI  WVFFFIA+VCL AGS+RNAYHT
Sbjct: 61  VLLASQLLIMVASRCLCCGRALTPSGSRSWAIFLFITTWVFFFIAQVCLLAGSVRNAYHT 120

Query: 121 KYRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARE 168
           KYR  FG+  PSC++LRKGVFGAGAAFI L  IVSELYYV  S+A++
Sbjct: 121 KYRVYFGNTSPSCRSLRKGVFGAGAAFIVLTGIVSELYYVTLSRAKD 166

[Close]

BLAST of Carg12753-RA vs. TAIR 10
Match: AT1G13380.1 (Protein of unknown function (DUF1218) )

HSP 1 Score: 170.2 bits (430), Expect = 1.5e-42
Identity = 80/167 (47.90%), Postives = 116/167 (69.46%), Query Frame = 0


Query: 2   ASKLVLIIVIVLDVIAFGLAVAAEQRRSTANVVPDSEKNYNYCVYDSDISTGYGVGAFLL 61
           AS LV I+V+ L ++AFG ++AAE+RRS    + D   N  +CVYDSD++TGYGVGAFL 
Sbjct: 6   ASTLVFILVVALSLVAFGFSIAAERRRSIGKSIQDPITNTTFCVYDSDVATGYGVGAFLF 65

Query: 62  LMASQALIMVASRCFCCGKSLSPGGSRAWAIILFIICWVFFFIAEVCLFAGSIRNAYHTK 121
           L++S++L+M  ++C C G+ L+PG  RAW+II FI  W+ F +AE C+ AG+ +NAYHTK
Sbjct: 66  LLSSESLLMSVTKCMCFGRPLAPGSDRAWSIIYFISSWMTFLVAEACVIAGATKNAYHTK 125

Query: 122 YRTMFGDNPPSCQTLRKGVFGAGAAFIFLNAIVSELYYVFYSKARES 169
           Y         SC +LRKG+F AGA FI    +++  YY++++K+  S
Sbjct: 126 Y---LSSQTFSCASLRKGIFIAGAVFIVATMVLNVYYYMYFTKSVSS 169

The following BLAST results are available for this feature:

BLAST of Carg12753-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022935762.1	6.9e-98	100.00	uncharacterized protein LOC111442584 [Cucurbita moschata] >XP_023535137.1 unchar...	[more]
XP_022976585.1	1.2e-97	98.91	uncharacterized protein LOC111476938 [Cucurbita maxima] >XP_022976586.1 uncharac...	[more]
XP_038900268.1	1.6e-94	95.65	uncharacterized protein LOC120087350 [Benincasa hispida]	[more]
XP_031740219.1	6.0e-94	94.57	uncharacterized protein LOC101208795 [Cucumis sativus] >XP_031740220.1 uncharact...	[more]
XP_008452482.1	5.6e-92	93.48	PREDICTED: uncharacterized protein LOC103493502 [Cucumis melo] >KAA0064347.1 DUF...	[more]

BLAST of Carg12753-RA vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg12753-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F6H9	3.3e-98	100.00	uncharacterized protein LOC111442584 OS=Cucurbita moschata OX=3662 GN=LOC1114425...	[more]
A0A6J1IJW2	5.7e-98	98.91	uncharacterized protein LOC111476938 OS=Cucurbita maxima OX=3661 GN=LOC111476938...	[more]
A0A0A0L071	2.9e-94	94.57	Fiber protein Fb34 OS=Cucumis sativus OX=3659 GN=Csa_4G638370 PE=4 SV=1	[more]
A0A5A7VBE4	2.7e-92	93.48	DUF1218 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BTB6	2.7e-92	93.48	uncharacterized protein LOC103493502 OS=Cucumis melo OX=3656 GN=LOC103493502 PE=...	[more]

BLAST of Carg12753-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27435.1	1.8e-67	72.83	Protein of unknown function (DUF1218)	[more]
AT1G52910.1	5.9e-63	67.65	Protein of unknown function (DUF1218)	[more]
AT3G15480.1	2.2e-62	68.24	Protein of unknown function (DUF1218)	[more]
AT1G61065.1	9.4e-61	68.26	Protein of unknown function (DUF1218)	[more]
AT1G13380.1	1.5e-42	47.90	Protein of unknown function (DUF1218)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009606	Modifying wall lignin-1/2	PFAM	PF06749	DUF1218	coord: 58..152 e-value: 1.1E-28 score: 99.6
None	No IPR available	PANTHER	PTHR31769:SF56	SUBFAMILY NOT NAMED	coord: 1..184
None	No IPR available	PANTHER	PTHR31769	OS07G0462200 PROTEIN-RELATED	coord: 1..184

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12753	Carg12753	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12753-RA:3	Carg12753-RA:3	exon
Carg12753-RA:2	Carg12753-RA:2	exon
Carg12753-RA:1	Carg12753-RA:1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12753-RA:three_prime_utr	Carg12753-RA:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12753-RA:cds	Carg12753-RA:cds_3	CDS
Carg12753-RA:cds	Carg12753-RA:cds_2	CDS
Carg12753-RA:cds	Carg12753-RA:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12753-RA:five_prime_utr	Carg12753-RA:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12753-RA	Carg12753-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane