Carg12202-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12202-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionchloroplast processing peptidase-like
LocationCarg_Chr11: 2679113 .. 2681560 (+)
Sequence length1554
RNA-Seq ExpressionCarg12202-RA
SyntenyCarg12202-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAACCCCAACAGCTTGAATTTGAAATTCATCACAGAGGCGCAATTCTGCCACAGAGGATGCCATCTCTTCATCTCCTCTCGTCAGTATCTTCTTTTGACAACCCTTCTCTACAACGTGCGCGCCTCTCGAAACCCGGTAACAGTTCTAATCCCCCAATTCATTCAAATGCCATTTCCCCAAAATTCGCACATGCCCACTTCGCCAATCGCTCTATTTCCCCTTCTGTAACTCTCAGTCAATTGCGTACTCAATCCCGAAGCAGTGGTTTTCTACCCTTTAAGCCTCCAAACGATGGAGCCCACTGGAAGAGGTGTTACTGTAGTGCTGTTAATGACTCTGACGAGAAAACTAGGTCAGTTTTGGACTCCAGTGGCGGCGGTGATGGAGGTGAAGGCGGCGATGGAAGTGATGATAATGGGAAGGTGGATAAGCAAAAGGGTTCTTCTGGGTTTCTGCCGGAATGGTTGAATTTAACTTCGGACGACGCCAAAACGGTCCTTGCAGCCATTGCAATATCTCTTGCCTTCAGGACGTTTATTGCAGAACCGAGGTACATTCCGTCGCTATCAATGTATCCTACCTTTGATGTTGGGGATCGACTTGTAGCAGAGAAGGTAGAAATCTTGTTCATTGTTCTTAATCGTATTATTCAACTGTTCTTCACGGTTATTAGAAAAAATTATACAGATTGGGATATGATATTCATAGTTAAAATTGCCTGAATCCCAAGTTCTTGGATTCAACTAAGCTTTGAAATAAAGGGATAAGATCTATGATTTCTATTCTGTGTATGAAATTTCTGGTGTTGTGGATCATGATGCTTGTTTCTTTTCAGGTCACATACTATTTCAGAAAGCCTTGTGCGAACGATATCGTGATATTTAAAAGCCCACCAGTGCTTCAGGAAGTAGGATATACAGATGAAGATGTCTTCATTAAAAGAGTTGTTGCTAAAGGAGGTGATACTGTGGAGGTCAGATTTACTAACGAGACCTTTTTTGTTTGTATCATTATCTGATATGTGTAATGGAGCCATTGATTTAGATCATCAAGAACAGATATCTATCTGTTTTTCAATGATATTGCAGGGTTCAAAAATGTAAATAACCGAATCTATCAGATGATCTAATTTTCTTGCACAACTTTAGTTAAAAGTTTTATGGTCGTAATCTCCAGCTTTCATTTGTGTTTTCAAGCTCAAGAAACAGCTTCACTCTTCACTTGGAACATTCACTGATTGCAGGTCCGGAAGGGCAAACTTATTGTCAATGGGGTCGAGAGAGACGAAAAGTTTATCCTTGAACCACCATCATACGACATGACTCCGGTTGTAAGTAGTTCCTTCTGTTTTCTGCTTCCTGCTTCGGTTTCGCATCTTTCTAAGATTTTCCTACCAAAATTATGAACACACAAGAATTGAGTACAAGCTGTGATCGTTTTTCCTTTTGGTTGAATGAAAGAGAGATTATTGCTTTTCCTTCAGTTTGTGAAATCTATATCAAGCATGGTTAAACATTACATCTCTTTCATTCTTATTTTGATGCAGCAAGTGCCAGAAAACTCGGTGTTTGTGATGGGCGATAATCGCAATAACAGCTATGATTCTCATGTGTGGTAAAGTCTTTCACTCATCACTTCACTAATACTGGGTTTAAGATGACTTTAAAAAGATGTGTATTTAAGTAAAAGACTTATGTTCAAATAAGCAACGATCTTGACAGTAGACTTCTATGTGCTTGTCTTTGAGTTTTTTTTCTTCCTTGTGCTGATAGCTAGCTACTACATATACCAAATTGTTCAGGGGTCCACTGCCAGCCAAAAATATAATAGGAAGATCATTATTCCGCTATTGGCCTCCGAACAGGATTGGTGGAACCGTTATCGAGCCTAGCTGTGCTACTGATAAACAAGAGGGCACTCCTTCCACACCAGAGATTTCCAGCCAACAGACCACAAATTCTCAACCGTAACAGTTTCGGTCATGTTCTTGTAAGTTCAGTTGGTTGTTCTGCAAATGCCTTATAGTTCCCATTCGCTCCCCTGTAATTTTGAAGCTCTTTTTTGTTGAATGAACTGGGCATTTTGTGCAAGGCCCTATTTACTGTTGATCTTGGTTTATGCAAGTTACTAGAGATGACTCTCAGTGTTGAGAGATCTGAACTAGTGACATTGCCATTCTTTGTGAACCCAATTGAACAGTACAAGCTAGTTATGATATAGGAATATGTGCTCTTGAAATTGAAAACTAGATAAATTGCTGCAGTAGATGTGATCAATACCATACAAGGAGGAGGTCGAATGTGCAAACCTTATGCTCCATCATGTCTGCAGATAAGTGAAGGTGTCTCATATGGCTTCTCCTGATATTTTTTATACTGATTCAAACATAATTTTATGGATACAATTCAATTACTATTATAACAAATTCAATCTTGTGTTTGTAATT

mRNA sequence

GGAACCCCAACAGCTTGAATTTGAAATTCATCACAGAGGCGCAATTCTGCCACAGAGGATGCCATCTCTTCATCTCCTCTCGTCAGTATCTTCTTTTGACAACCCTTCTCTACAACGTGCGCGCCTCTCGAAACCCGGTAACAGTTCTAATCCCCCAATTCATTCAAATGCCATTTCCCCAAAATTCGCACATGCCCACTTCGCCAATCGCTCTATTTCCCCTTCTGTAACTCTCAGTCAATTGCGTACTCAATCCCGAAGCAGTGGTTTTCTACCCTTTAAGCCTCCAAACGATGGAGCCCACTGGAAGAGGTGTTACTGTAGTGCTGTTAATGACTCTGACGAGAAAACTAGGTCAGTTTTGGACTCCAGTGGCGGCGGTGATGGAGGTGAAGGCGGCGATGGAAGTGATGATAATGGGAAGGTGGATAAGCAAAAGGGTTCTTCTGGGTTTCTGCCGGAATGGTTGAATTTAACTTCGGACGACGCCAAAACGGTCCTTGCAGCCATTGCAATATCTCTTGCCTTCAGGACGTTTATTGCAGAACCGAGGTACATTCCGTCGCTATCAATGTATCCTACCTTTGATGTTGGGGATCGACTTGTAGCAGAGAAGGTCACATACTATTTCAGAAAGCCTTGTGCGAACGATATCGTGATATTTAAAAGCCCACCAGTGCTTCAGGAAGTAGGATATACAGATGAAGATGTCTTCATTAAAAGAGTTGTTGCTAAAGGAGGTGATACTGTGGAGGTCCGGAAGGGCAAACTTATTGTCAATGGGGTCGAGAGAGACGAAAAGTTTATCCTTGAACCACCATCATACGACATGACTCCGGTTCAAGTGCCAGAAAACTCGGTGTTTGTGATGGGCGATAATCGCAATAACAGCTATGATTCTCATGTGTGGGGTCCACTGCCAGCCAAAAATATAATAGGAAGATCATTATTCCGCTATTGGCCTCCGAACAGGATTGGTGGAACCGTTATCGAGCCTAGCTGTGCTACTGATAAACAAGAGGGCACTCCTTCCACACCAGAGATTTCCAGCCAACAGACCACAAATTCTCAACCGTAACAGTTTCGGTCATGTTCTTGTAAGTTCAGTTGGTTGTTCTGCAAATGCCTTATAGTTCCCATTCGCTCCCCTGTAATTTTGAAGCTCTTTTTTGTTGAATGAACTGGGCATTTTGTGCAAGGCCCTATTTACTGTTGATCTTGGTTTATGCAAGTTACTAGAGATGACTCTCAGTGTTGAGAGATCTGAACTAGTGACATTGCCATTCTTTGTGAACCCAATTGAACAGTACAAGCTAGTTATGATATAGGAATATGTGCTCTTGAAATTGAAAACTAGATAAATTGCTGCAGTAGATGTGATCAATACCATACAAGGAGGAGGTCGAATGTGCAAACCTTATGCTCCATCATGTCTGCAGATAAGTGAAGGTGTCTCATATGGCTTCTCCTGATATTTTTTATACTGATTCAAACATAATTTTATGGATACAATTCAATTACTATTATAACAAATTCAATCTTGTGTTTGTAATT

Coding sequence (CDS)

ATGCCATCTCTTCATCTCCTCTCGTCAGTATCTTCTTTTGACAACCCTTCTCTACAACGTGCGCGCCTCTCGAAACCCGGTAACAGTTCTAATCCCCCAATTCATTCAAATGCCATTTCCCCAAAATTCGCACATGCCCACTTCGCCAATCGCTCTATTTCCCCTTCTGTAACTCTCAGTCAATTGCGTACTCAATCCCGAAGCAGTGGTTTTCTACCCTTTAAGCCTCCAAACGATGGAGCCCACTGGAAGAGGTGTTACTGTAGTGCTGTTAATGACTCTGACGAGAAAACTAGGTCAGTTTTGGACTCCAGTGGCGGCGGTGATGGAGGTGAAGGCGGCGATGGAAGTGATGATAATGGGAAGGTGGATAAGCAAAAGGGTTCTTCTGGGTTTCTGCCGGAATGGTTGAATTTAACTTCGGACGACGCCAAAACGGTCCTTGCAGCCATTGCAATATCTCTTGCCTTCAGGACGTTTATTGCAGAACCGAGGTACATTCCGTCGCTATCAATGTATCCTACCTTTGATGTTGGGGATCGACTTGTAGCAGAGAAGGTCACATACTATTTCAGAAAGCCTTGTGCGAACGATATCGTGATATTTAAAAGCCCACCAGTGCTTCAGGAAGTAGGATATACAGATGAAGATGTCTTCATTAAAAGAGTTGTTGCTAAAGGAGGTGATACTGTGGAGGTCCGGAAGGGCAAACTTATTGTCAATGGGGTCGAGAGAGACGAAAAGTTTATCCTTGAACCACCATCATACGACATGACTCCGGTTCAAGTGCCAGAAAACTCGGTGTTTGTGATGGGCGATAATCGCAATAACAGCTATGATTCTCATGTGTGGGGTCCACTGCCAGCCAAAAATATAATAGGAAGATCATTATTCCGCTATTGGCCTCCGAACAGGATTGGTGGAACCGTTATCGAGCCTAGCTGTGCTACTGATAAACAAGAGGGCACTCCTTCCACACCAGAGATTTCCAGCCAACAGACCACAAATTCTCAACCGTAA

Protein sequence

MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLSQLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDNGKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRYWPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP
Homology
BLAST of Carg12202-RA vs. NCBI nr
Match: KAG6587965.1 (Chloroplast processing peptidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021855.1 Chloroplast processing peptidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 679.1 bits (1751), Expect = 2.0e-191
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60
           MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS
Sbjct: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60

Query: 61  QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120
           QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN
Sbjct: 61  QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120

Query: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180
           GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD
Sbjct: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180

Query: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240
           RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV
Sbjct: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240

Query: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300
           NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY
Sbjct: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300

Query: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP
Sbjct: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 339

BLAST of Carg12202-RA vs. NCBI nr
Match: XP_022933769.1 (chloroplast processing peptidase-like [Cucurbita moschata])

HSP 1 Score: 674.5 bits (1739), Expect = 4.9e-190
Identity = 337/339 (99.41%), Postives = 337/339 (99.41%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60
           MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS
Sbjct: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60

Query: 61  QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120
           QLR QSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGE GDGSDDN
Sbjct: 61  QLRIQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEAGDGSDDN 120

Query: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180
           GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD
Sbjct: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180

Query: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240
           RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV
Sbjct: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240

Query: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300
           NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY
Sbjct: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300

Query: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP
Sbjct: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 339

BLAST of Carg12202-RA vs. NCBI nr
Match: XP_023529432.1 (chloroplast processing peptidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 662.9 bits (1709), Expect = 1.5e-186
Identity = 332/339 (97.94%), Postives = 334/339 (98.53%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60
           MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS
Sbjct: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60

Query: 61  QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120
           QLR QSRSS FLPFKPPNDGAHWKR YCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN
Sbjct: 61  QLRIQSRSSSFLPFKPPNDGAHWKRGYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120

Query: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180
           GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD
Sbjct: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180

Query: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240
           RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV
Sbjct: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240

Query: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300
           NGVERDEKFILEPPSYDMTPV+VPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY
Sbjct: 241 NGVERDEKFILEPPSYDMTPVEVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300

Query: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           WPPNRIGGTVIEPSCA DKQEGTPSTPEISSQQT+ SQP
Sbjct: 301 WPPNRIGGTVIEPSCAADKQEGTPSTPEISSQQTSTSQP 339

BLAST of Carg12202-RA vs. NCBI nr
Match: XP_022973164.1 (chloroplast processing peptidase-like [Cucurbita maxima])

HSP 1 Score: 659.1 bits (1699), Expect = 2.1e-185
Identity = 331/339 (97.64%), Postives = 333/339 (98.23%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60
           M SLHLLSSVSSFDNPSLQRARLSKPG SSNPPIHSNAISPKFAHAHFANRSISPSVTLS
Sbjct: 1   MLSLHLLSSVSSFDNPSLQRARLSKPGISSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60

Query: 61  QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120
           QLR QSRSS FLPFKPPNDGAHWKR YCSAVNDSDEKTRSVLDS GGGDGGEGGDGSDDN
Sbjct: 61  QLRIQSRSSSFLPFKPPNDGAHWKRGYCSAVNDSDEKTRSVLDSGGGGDGGEGGDGSDDN 120

Query: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180
           GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD
Sbjct: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180

Query: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240
           RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV
Sbjct: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240

Query: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300
           NGVERDEKF+LEPPSYDM+PVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY
Sbjct: 241 NGVERDEKFVLEPPSYDMSPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300

Query: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP
Sbjct: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 339

BLAST of Carg12202-RA vs. NCBI nr
Match: XP_038878814.1 (chloroplast processing peptidase [Benincasa hispida])

HSP 1 Score: 561.2 bits (1445), Expect = 6.0e-156
Identity = 293/344 (85.17%), Postives = 301/344 (87.50%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPI---HSNAISPKFAHAHFANRSISPSV 60
           M SLHLLSSV SFDN SLQR RLSKP N SN PI   HS AISPKF H H ANRSI P V
Sbjct: 1   MLSLHLLSSVPSFDNTSLQRTRLSKPNNPSNFPILNLHSIAISPKFVHIHIANRSIFPFV 60

Query: 61  TLSQLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGS 120
           T S+L  QS SS FL FKPP+DG H KR  CSAVNDSDEKTRSV+D+ GGG GG GG G 
Sbjct: 61  TRSKLSIQSGSSSFLAFKPPSDGTHLKRGCCSAVNDSDEKTRSVVDNGGGGGGGGGGGGD 120

Query: 121 --DDNGKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPT 180
             +DNGKV+ QK SSGFLPEWLNLTSDDAKTV AAIAISLAFRTFIAEPRYIPSLSMYPT
Sbjct: 121 EREDNGKVENQKDSSGFLPEWLNLTSDDAKTVFAAIAISLAFRTFIAEPRYIPSLSMYPT 180

Query: 181 FDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRK 240
           FDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAK GDTVEVR 
Sbjct: 181 FDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKQGDTVEVRN 240

Query: 241 GKLIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGR 300
           GKLIVNGVER+EKFILEPPSYDMTP+QVPEN VFVMGDNRNNSYDSHVWGPLPAKNIIGR
Sbjct: 241 GKLIVNGVERNEKFILEPPSYDMTPIQVPENMVFVMGDNRNNSYDSHVWGPLPAKNIIGR 300

Query: 301 SLFRYWPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           SLFRYWPPNRIGGTV EPSCA DKQEG PSTPEISS QTTNSQP
Sbjct: 301 SLFRYWPPNRIGGTVSEPSCAIDKQEGIPSTPEISSLQTTNSQP 344

BLAST of Carg12202-RA vs. ExPASy Swiss-Prot
Match: Q8H0W1 (Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 SV=2)

HSP 1 Score: 356.3 bits (913), Expect = 3.8e-97
Identity = 175/233 (75.11%), Postives = 195/233 (83.69%), Query Frame = 0

Query: 88  CSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDNGKVDKQKGSSGFLPEWLNLTSDDAKTV 147
           C  + DS E T+S   S   GDGG G  G DD G+V+++   +   PEWL+ TSDDA+TV
Sbjct: 63  CYGIKDSSETTKSA-PSLDSGDGGGGDGGDDDKGEVEEK---NRLFPEWLDFTSDDAQTV 122

Query: 148 LAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKSPPV 207
             AIA+SLAFR FIAEPRYIPSLSMYPTFDVGDRLVAEKV+YYFRKPCANDIVIFKSPPV
Sbjct: 123 FVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKVSYYFRKPCANDIVIFKSPPV 182

Query: 208 LQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVPENS 267
           LQEVGYTD DVFIKR+VAK GD VEV  GKL+VNGV R+EKFILEPP Y+MTP++VPENS
Sbjct: 183 LQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNEKFILEPPGYEMTPIRVPENS 242

Query: 268 VFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRYWPPNRIGGTVIEPSCATDKQ 321
           VFVMGDNRNNSYDSHVWGPLP KNIIGRS+FRYWPPNR+ GTV+E  CA DKQ
Sbjct: 243 VFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRVSGTVLEGGCAVDKQ 291

BLAST of Carg12202-RA vs. ExPASy Swiss-Prot
Match: O04348 (Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP1 PE=2 SV=2)

HSP 1 Score: 237.7 bits (605), Expect = 2.0e-61
Identity = 113/212 (53.30%), Postives = 154/212 (72.64%), Query Frame = 0

Query: 109 DGGEGGDGSDDNGKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIP 168
           D  +GG   DD+   + + G SG++ + L++ S+DAK    A+ +S+ FR+ +AEP+ IP
Sbjct: 122 DVDKGGTVCDDDDDKESRNGGSGWVNKLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIP 181

Query: 169 SLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVL---QEVGYTDEDVFIKRVVA 228
           S SMYPT D GDR++AEKV+Y+FRKP  +DIVIFK+PP+L    E GY+  DVFIKR+VA
Sbjct: 182 STSMYPTLDKGDRVMAEKVSYFFRKPEVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVA 241

Query: 229 KGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWG 288
             GD VEVR GKL VN + ++E F+LEP SY+M P+ VP+  VFV+GDNRN S+DSH WG
Sbjct: 242 SEGDWVEVRDGKLFVNDIVQEEDFVLEPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWG 301

Query: 289 PLPAKNIIGRSLFRYWPPNRIGGTVIEPSCAT 318
           PLP +NI+GRS+FRYWPP+++  T+      T
Sbjct: 302 PLPIENIVGRSVFRYWPPSKVSDTIYHDQAIT 333

BLAST of Carg12202-RA vs. ExPASy Swiss-Prot
Match: Q9M9Z2 (Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP2 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 122/246 (49.59%), Postives = 161/246 (65.45%), Query Frame = 0

Query: 70  GFLPFK-----PPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDNGKVD 129
           G  PFK     P   G+ W  C   A   +D     + +   GG   +     + + KV 
Sbjct: 115 GISPFKTSSVIPFLRGSKWMPCSIPATLSTD-----IAEVDRGGKVCDPKVKLELSDKV- 174

Query: 130 KQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGDRLVA 189
              G +G++ + LN+ S+DAK    A+ +SL FR+ +AEP+ IPS SM PT DVGDR++A
Sbjct: 175 -SNGGNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDRVIA 234

Query: 190 EKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIVNGVE 249
           EKV+Y+FRKP  +DIVIFK+PP+L E GY+  DVFIKR+VA  GD VEV  GKL+VN   
Sbjct: 235 EKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVNDTV 294

Query: 250 RDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRYWPPN 309
           + E F+LEP  Y+M P+ VPE  VFV+GDNRN S+DSH WGPLP KNIIGRS+FRYWPP+
Sbjct: 295 QAEDFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRYWPPS 353

Query: 310 RIGGTV 311
           ++   +
Sbjct: 355 KVSDII 353

BLAST of Carg12202-RA vs. ExPASy Swiss-Prot
Match: P72660 (Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB1 PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.8e-45
Identity = 88/175 (50.29%), Postives = 117/175 (66.86%), Query Frame = 0

Query: 133 LPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGDRLVAEKVTYYFR 192
           +P       ++   ++ A+ ++L  R F+AEPRYIPS SM PT + GDRLV EKV+Y+F 
Sbjct: 6   IPSPWQFIKENIPLLMVALVLALLLRFFVAEPRYIPSDSMLPTLEQGDRLVVEKVSYHFH 65

Query: 193 KPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIVNGVERDEKFILE 252
            P   DI++F  P +LQ  GY     FIKRV+A  G TVEV  G +  +G    E++ILE
Sbjct: 66  PPQVGDIIVFHPPELLQVQGYDLGQAFIKRVIALPGQTVEVNNGIVYRDGQPLQEEYILE 125

Query: 253 PPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRYWPPNRIG 308
           PP Y++  V+VP+  VFVMGDNRNNS DSHVWG LP +NIIG +LFR++P +R G
Sbjct: 126 PPQYNLPAVRVPDGQVFVMGDNRNNSNDSHVWGFLPQQNIIGHALFRFFPASRWG 180

BLAST of Carg12202-RA vs. ExPASy Swiss-Prot
Match: Q51876 (Signal peptidase I OS=Phormidium laminosum OX=32059 GN=lepB PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.3e-43
Identity = 79/170 (46.47%), Postives = 114/170 (67.06%), Query Frame = 0

Query: 145 KTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKS 204
           KT+  ++ ++L  RTF+AE RYIPS SM PT +V DRL+ EK++Y+F  P   DI++F  
Sbjct: 33  KTIGLSVVLALGIRTFVAEARYIPSESMLPTLEVNDRLIVEKISYHFNPPRRGDIIVFHP 92

Query: 205 PPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVP 264
              L++   +  + FIKRV+   G+TV+V  G++++NG   +E +I  PP Y   P +VP
Sbjct: 93  TEALKQQNPSLNEAFIKRVIGLPGETVQVTGGRVLINGQPLEENYIQSPPDYQWGPEKVP 152

Query: 265 ENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRYWPPNRIGGTVIEPS 315
            +S  V+GDNRNNSYDSH WG +P +NIIGR++ R+WP NR+G     PS
Sbjct: 153 ADSFLVLGDNRNNSYDSHFWGYVPRQNIIGRAVVRFWPVNRLGELGPPPS 202

BLAST of Carg12202-RA vs. ExPASy TrEMBL
Match: A0A6J1F0N9 (chloroplast processing peptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111441081 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.3e-190
Identity = 337/339 (99.41%), Postives = 337/339 (99.41%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60
           MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS
Sbjct: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60

Query: 61  QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120
           QLR QSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGE GDGSDDN
Sbjct: 61  QLRIQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEAGDGSDDN 120

Query: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180
           GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD
Sbjct: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180

Query: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240
           RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV
Sbjct: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240

Query: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300
           NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY
Sbjct: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300

Query: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP
Sbjct: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 339

BLAST of Carg12202-RA vs. ExPASy TrEMBL
Match: A0A6J1IDR5 (chloroplast processing peptidase-like OS=Cucurbita maxima OX=3661 GN=LOC111471667 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.0e-185
Identity = 331/339 (97.64%), Postives = 333/339 (98.23%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60
           M SLHLLSSVSSFDNPSLQRARLSKPG SSNPPIHSNAISPKFAHAHFANRSISPSVTLS
Sbjct: 1   MLSLHLLSSVSSFDNPSLQRARLSKPGISSNPPIHSNAISPKFAHAHFANRSISPSVTLS 60

Query: 61  QLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDN 120
           QLR QSRSS FLPFKPPNDGAHWKR YCSAVNDSDEKTRSVLDS GGGDGGEGGDGSDDN
Sbjct: 61  QLRIQSRSSSFLPFKPPNDGAHWKRGYCSAVNDSDEKTRSVLDSGGGGDGGEGGDGSDDN 120

Query: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180
           GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD
Sbjct: 121 GKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGD 180

Query: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240
           RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV
Sbjct: 181 RLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIV 240

Query: 241 NGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300
           NGVERDEKF+LEPPSYDM+PVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY
Sbjct: 241 NGVERDEKFVLEPPSYDMSPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRY 300

Query: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP
Sbjct: 301 WPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 339

BLAST of Carg12202-RA vs. ExPASy TrEMBL
Match: A0A5D3DZ24 (Chloroplast processing peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00220 PE=4 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 8.2e-151
Identity = 285/342 (83.33%), Postives = 291/342 (85.09%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPI---HSNAISPKFAHAHFANRSISPSV 60
           M S HL SS  SF N SLQR  L KP + S  PI   HS   SPKFAH HFANRSI    
Sbjct: 1   MLSFHLFSSAPSFHNTSLQRTGLPKPNSPSYLPILNLHSIPNSPKFAHTHFANRSI---- 60

Query: 61  TLSQLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGS 120
                  QS +  FL FKPP+DG H KR  CSAVNDSDEKTRSVLD+ GGGDGG GGDGS
Sbjct: 61  -------QSGTYSFLAFKPPSDGTHLKRGRCSAVNDSDEKTRSVLDTGGGGDGGGGGDGS 120

Query: 121 DDNGKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFD 180
           DDNGKV+ QK SSGFLPEWLNLTSDDAKTV AAIAISLAFRTFIAEPRYIPSLSMYPTFD
Sbjct: 121 DDNGKVENQKDSSGFLPEWLNLTSDDAKTVFAAIAISLAFRTFIAEPRYIPSLSMYPTFD 180

Query: 181 VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGK 240
           VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAK GDTVEVRKGK
Sbjct: 181 VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKEGDTVEVRKGK 240

Query: 241 LIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL 300
           LIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL
Sbjct: 241 LIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL 300

Query: 301 FRYWPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           FRYWPPNRIGGTV EPSCA DK+E  PSTPEISS QTTNSQP
Sbjct: 301 FRYWPPNRIGGTVSEPSCAIDKEESIPSTPEISSLQTTNSQP 331

BLAST of Carg12202-RA vs. ExPASy TrEMBL
Match: A0A1S3CJ67 (chloroplast processing peptidase OS=Cucumis melo OX=3656 GN=LOC103501565 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.8e-150
Identity = 284/342 (83.04%), Postives = 291/342 (85.09%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPI---HSNAISPKFAHAHFANRSISPSV 60
           M S HL SS  SF N SLQR  L KP + S  PI   HS   SPKFAH HFANRSI    
Sbjct: 1   MLSFHLFSSAPSFHNTSLQRTGLPKPNSPSYLPILNLHSIPNSPKFAHTHFANRSI---- 60

Query: 61  TLSQLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGS 120
                  QS +  FL FKPP+DG H KR  CSAVNDSDEKTRSVLD+ GGGDGG GGDGS
Sbjct: 61  -------QSGTYSFLAFKPPSDGTHLKRGRCSAVNDSDEKTRSVLDTGGGGDGGGGGDGS 120

Query: 121 DDNGKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFD 180
           DDNGKV+ QK SSGFLPEWLNLTSDDAKTV AAIAISLAFRTFIAEPRYIPSLSMYPTFD
Sbjct: 121 DDNGKVENQKDSSGFLPEWLNLTSDDAKTVFAAIAISLAFRTFIAEPRYIPSLSMYPTFD 180

Query: 181 VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGK 240
           VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAK GDTVEVRKGK
Sbjct: 181 VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKEGDTVEVRKGK 240

Query: 241 LIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL 300
           LIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL
Sbjct: 241 LIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL 300

Query: 301 FRYWPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           FRYWPPNRIGGTV +PSCA DK+E  PSTPEISS QTTNSQP
Sbjct: 301 FRYWPPNRIGGTVSDPSCAIDKEESIPSTPEISSLQTTNSQP 331

BLAST of Carg12202-RA vs. ExPASy TrEMBL
Match: A0A6J1CTI2 (chloroplast processing peptidase OS=Momordica charantia OX=3673 GN=LOC111014443 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.4e-150
Identity = 280/342 (81.87%), Postives = 297/342 (86.84%), Query Frame = 0

Query: 1   MPSLHLLSSVSSFDNPSLQRARLSKPGNSSNPPI---HSNAISPKFAHAHFANRSISPSV 60
           M SLHLLSSV  FDN + QR R+SKP N+SN  I   HS AI+ KFAHA FA+R I P  
Sbjct: 1   MLSLHLLSSVPLFDNHAPQRTRVSKPNNTSNSSILSLHSIAIAQKFAHARFASRYIFPYA 60

Query: 61  TLSQLRTQSRSSGFLPFKPPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGS 120
              +   QS +S F  FKPP+DG H KR  C AVNDSDEKTRSVLD+ GGGDGG GGD S
Sbjct: 61  NCRKFSIQSGNSSFRAFKPPSDGTHSKRGRCGAVNDSDEKTRSVLDTGGGGDGGGGGDES 120

Query: 121 DDNGKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFD 180
           +D+GKV+K+KGSSGFLPEWLNLTSDDAKTV AAIAISLAFRTFIAEPRYIPSLSMYPTFD
Sbjct: 121 EDSGKVEKEKGSSGFLPEWLNLTSDDAKTVFAAIAISLAFRTFIAEPRYIPSLSMYPTFD 180

Query: 181 VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGK 240
           VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAK GDTVEV+KGK
Sbjct: 181 VGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKEGDTVEVQKGK 240

Query: 241 LIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL 300
           LIVNGVER EKFILEPPSY+MTPVQVP+NSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL
Sbjct: 241 LIVNGVERSEKFILEPPSYNMTPVQVPKNSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSL 300

Query: 301 FRYWPPNRIGGTVIEPSCATDKQEGTPSTPEISSQQTTNSQP 340
           FRYWPPNRIGGTV EPSCA DKQE  PSTPEISS QT+NSQP
Sbjct: 301 FRYWPPNRIGGTVSEPSCAVDKQESIPSTPEISSLQTSNSQP 342

BLAST of Carg12202-RA vs. TAIR 10
Match: AT3G24590.1 (plastidic type i signal peptidase 1 )

HSP 1 Score: 356.3 bits (913), Expect = 2.7e-98
Identity = 175/233 (75.11%), Postives = 195/233 (83.69%), Query Frame = 0

Query: 88  CSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDNGKVDKQKGSSGFLPEWLNLTSDDAKTV 147
           C  + DS E T+S   S   GDGG G  G DD G+V+++   +   PEWL+ TSDDA+TV
Sbjct: 63  CYGIKDSSETTKSA-PSLDSGDGGGGDGGDDDKGEVEEK---NRLFPEWLDFTSDDAQTV 122

Query: 148 LAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKSPPV 207
             AIA+SLAFR FIAEPRYIPSLSMYPTFDVGDRLVAEKV+YYFRKPCANDIVIFKSPPV
Sbjct: 123 FVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKVSYYFRKPCANDIVIFKSPPV 182

Query: 208 LQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVPENS 267
           LQEVGYTD DVFIKR+VAK GD VEV  GKL+VNGV R+EKFILEPP Y+MTP++VPENS
Sbjct: 183 LQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNEKFILEPPGYEMTPIRVPENS 242

Query: 268 VFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRYWPPNRIGGTVIEPSCATDKQ 321
           VFVMGDNRNNSYDSHVWGPLP KNIIGRS+FRYWPPNR+ GTV+E  CA DKQ
Sbjct: 243 VFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRVSGTVLEGGCAVDKQ 291

BLAST of Carg12202-RA vs. TAIR 10
Match: AT2G30440.1 (thylakoid processing peptide )

HSP 1 Score: 237.7 bits (605), Expect = 1.4e-62
Identity = 113/212 (53.30%), Postives = 154/212 (72.64%), Query Frame = 0

Query: 109 DGGEGGDGSDDNGKVDKQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIP 168
           D  +GG   DD+   + + G SG++ + L++ S+DAK    A+ +S+ FR+ +AEP+ IP
Sbjct: 122 DVDKGGTVCDDDDDKESRNGGSGWVNKLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIP 181

Query: 169 SLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVL---QEVGYTDEDVFIKRVVA 228
           S SMYPT D GDR++AEKV+Y+FRKP  +DIVIFK+PP+L    E GY+  DVFIKR+VA
Sbjct: 182 STSMYPTLDKGDRVMAEKVSYFFRKPEVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVA 241

Query: 229 KGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWG 288
             GD VEVR GKL VN + ++E F+LEP SY+M P+ VP+  VFV+GDNRN S+DSH WG
Sbjct: 242 SEGDWVEVRDGKLFVNDIVQEEDFVLEPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWG 301

Query: 289 PLPAKNIIGRSLFRYWPPNRIGGTVIEPSCAT 318
           PLP +NI+GRS+FRYWPP+++  T+      T
Sbjct: 302 PLPIENIVGRSVFRYWPPSKVSDTIYHDQAIT 333

BLAST of Carg12202-RA vs. TAIR 10
Match: AT1G06870.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 228.4 bits (581), Expect = 8.6e-60
Identity = 122/246 (49.59%), Postives = 161/246 (65.45%), Query Frame = 0

Query: 70  GFLPFK-----PPNDGAHWKRCYCSAVNDSDEKTRSVLDSSGGGDGGEGGDGSDDNGKVD 129
           G  PFK     P   G+ W  C   A   +D     + +   GG   +     + + KV 
Sbjct: 115 GISPFKTSSVIPFLRGSKWMPCSIPATLSTD-----IAEVDRGGKVCDPKVKLELSDKV- 174

Query: 130 KQKGSSGFLPEWLNLTSDDAKTVLAAIAISLAFRTFIAEPRYIPSLSMYPTFDVGDRLVA 189
              G +G++ + LN+ S+DAK    A+ +SL FR+ +AEP+ IPS SM PT DVGDR++A
Sbjct: 175 -SNGGNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDRVIA 234

Query: 190 EKVTYYFRKPCANDIVIFKSPPVLQEVGYTDEDVFIKRVVAKGGDTVEVRKGKLIVNGVE 249
           EKV+Y+FRKP  +DIVIFK+PP+L E GY+  DVFIKR+VA  GD VEV  GKL+VN   
Sbjct: 235 EKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVNDTV 294

Query: 250 RDEKFILEPPSYDMTPVQVPENSVFVMGDNRNNSYDSHVWGPLPAKNIIGRSLFRYWPPN 309
           + E F+LEP  Y+M P+ VPE  VFV+GDNRN S+DSH WGPLP KNIIGRS+FRYWPP+
Sbjct: 295 QAEDFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRYWPPS 353

Query: 310 RIGGTV 311
           ++   +
Sbjct: 355 KVSDII 353

BLAST of Carg12202-RA vs. TAIR 10
Match: AT1G23465.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-10
Identity = 47/145 (32.41%), Postives = 71/145 (48.97%), Query Frame = 0

Query: 155 LAFRTFIAEPRYIPSLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYT 214
           L F  +   P  IP+L  +P+   G+ L+AE+++  ++KP   DIV+ +SP         
Sbjct: 37  LGFMAYAYGPSMIPTL--HPS---GNMLLAERISKRYQKPSRGDIVVIRSPE-------N 96

Query: 215 DEDVFIKRVVAKGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDN 274
                IKRVV   GD +       +++ V+ DE             + VP+  VFV GD 
Sbjct: 97  PNKTPIKRVVGVEGDCI-----SFVIDPVKSDES----------QTIVVPKGHVFVQGDY 154

Query: 275 RNNSYDSHVWGPLPAKNIIGRSLFR 300
            +NS DS  +GP+P   I GR L+R
Sbjct: 157 THNSRDSRNFGPVPYGLIQGRVLWR 154

BLAST of Carg12202-RA vs. TAIR 10
Match: AT1G29960.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 62.8 bits (151), Expect = 6.2e-10
Identity = 45/148 (30.41%), Postives = 70/148 (47.30%), Query Frame = 0

Query: 155 LAFRTFIAEPRYIPSLSMYPTFDVGDRLVAEKVTYYFRKPCANDIVIFKSPPVLQEVGYT 214
           L F  +   P   P+L  +P+   G+ L+AE+++  ++KP   DIV+ +SP         
Sbjct: 37  LGFMAYAYGPSMTPTL--HPS---GNVLLAERISKRYQKPSRGDIVVIRSPE-------N 96

Query: 215 DEDVFIKRVVAKGGDTVEVRKGKLIVNGVERDEKFILEPPSYDMTPVQVPENSVFVMGDN 274
                IKRV+   GD +       +++  + DE             + VP+  VFV GD 
Sbjct: 97  PNKTPIKRVIGIEGDCI-----SFVIDSRKSDES----------QTIVVPKGHVFVQGDY 156

Query: 275 RNNSYDSHVWGPLPAKNIIGRSLFRYWP 303
            +NS DS  +G +P   I GR L+R WP
Sbjct: 157 THNSRDSRNFGTVPYGLIQGRVLWRVWP 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6587965.12.0e-191100.00Chloroplast processing peptidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022933769.14.9e-19099.41chloroplast processing peptidase-like [Cucurbita moschata][more]
XP_023529432.11.5e-18697.94chloroplast processing peptidase-like [Cucurbita pepo subsp. pepo][more]
XP_022973164.12.1e-18597.64chloroplast processing peptidase-like [Cucurbita maxima][more]
XP_038878814.16.0e-15685.17chloroplast processing peptidase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8H0W13.8e-9775.11Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 S... [more]
O043482.0e-6153.30Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9M9Z21.2e-5849.59Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thalia... [more]
P726605.8e-4550.29Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX... [more]
Q518769.3e-4346.47Signal peptidase I OS=Phormidium laminosum OX=32059 GN=lepB PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F0N92.3e-19099.41chloroplast processing peptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111441... [more]
A0A6J1IDR51.0e-18597.64chloroplast processing peptidase-like OS=Cucurbita maxima OX=3661 GN=LOC11147166... [more]
A0A5D3DZ248.2e-15183.33Chloroplast processing peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CJ671.8e-15083.04chloroplast processing peptidase OS=Cucumis melo OX=3656 GN=LOC103501565 PE=4 SV... [more]
A0A6J1CTI22.4e-15081.87chloroplast processing peptidase OS=Momordica charantia OX=3673 GN=LOC111014443 ... [more]
Match NameE-valueIdentityDescription
AT3G24590.12.7e-9875.11plastidic type i signal peptidase 1 [more]
AT2G30440.11.4e-6253.30thylakoid processing peptide [more]
AT1G06870.18.6e-6049.59Peptidase S24/S26A/S26B/S26C family protein [more]
AT1G23465.11.6e-1032.41Peptidase S24/S26A/S26B/S26C family protein [more]
AT1G29960.16.2e-1030.41Peptidase S24/S26A/S26B/S26C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000223Peptidase S26A, signal peptidase IPRINTSPR00727LEADERPTASEcoord: 160..176
score: 45.24
coord: 219..231
score: 53.66
coord: 263..282
score: 57.86
IPR000223Peptidase S26A, signal peptidase ITIGRFAMTIGR02227TIGR02227coord: 145..303
e-value: 1.7E-39
score: 133.3
IPR000223Peptidase S26A, signal peptidase IPANTHERPTHR43390SIGNAL PEPTIDASE Icoord: 56..314
NoneNo IPR availableGENE3D2.10.109.10Umud Fragment, subunit Acoord: 157..312
e-value: 9.4E-36
score: 124.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 316..339
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..339
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..125
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availablePANTHERPTHR43390:SF2THYLAKOIDAL PROCESSING PEPTIDASE 2, CHLOROPLASTIC-RELATEDcoord: 56..314
IPR019533Peptidase S26PFAMPF10502Peptidase_S26coord: 144..301
e-value: 3.5E-43
score: 147.5
IPR019533Peptidase S26CDDcd06530S26_SPase_Icoord: 163..295
e-value: 2.04919E-25
score: 95.7303
IPR019756Peptidase S26A, signal peptidase I, serine active sitePROSITEPS00501SPASE_I_1coord: 169..176
IPR019757Peptidase S26A, signal peptidase I, lysine active sitePROSITEPS00760SPASE_I_2coord: 221..233
IPR019758Peptidase S26A, signal peptidase I, conserved sitePROSITEPS00761SPASE_I_3coord: 268..281
IPR036286LexA/Signal peptidase-like superfamilySUPERFAMILY51306LexA/Signal peptidasecoord: 158..309

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12202Carg12202gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12202-RA:1Carg12202-RA:1exon
Carg12202-RA:2Carg12202-RA:2exon
Carg12202-RA:3Carg12202-RA:3exon
Carg12202-RA:4Carg12202-RA:4exon
Carg12202-RA:5Carg12202-RA:5exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12202-RA:five_prime_utrCarg12202-RA:five_prime_utrfive_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12202-RA:cdsCarg12202-RA:cdsCDS
Carg12202-RA:cdsCarg12202-RA:cds_2CDS
Carg12202-RA:cdsCarg12202-RA:cds_3CDS
Carg12202-RA:cdsCarg12202-RA:cds_4CDS
Carg12202-RA:cdsCarg12202-RA:cds_5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12202-RA:three_prime_utrCarg12202-RA:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12202-RACarg12202-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0006465 signal peptide processing
biological_process GO:0010027 thylakoid membrane organization
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005887 integral component of plasma membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity