Carg12181-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12181-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionVIN3-like protein 1
LocationCarg_Chr11: 2589801 .. 2593725 (+)
Sequence length2542
RNA-Seq ExpressionCarg12181-RA
SyntenyCarg12181-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAAGACTCTTTCTCTGTCTCTCTGTCTCTCTCTCTCTCTTCTTCTTCTTTCTCTCTCTAGCTTCATGCTTGCGCAGAACAAATTATTCGGTTTCGGCGCATCGAAGCCCTAGATTCGTCCCCATTCTTCGCCGGTACGTCCCGAATATTTCTCTTTTATTTATATCGTTTCATTCAAACGATTTCCTTGGTTCTTTGGGGCTGTTTCTGTGGCAGATCCATTGATTTTCGAGCTTCCTTCTTGGTGTTGTTCTTTTTTTTCTTTTTCTTTCTGTTTTTCTATCCGTATTGTGTTAGTATTCCGCAGCGCTGCTATATATGTTTTTCTGTTTAATTCGCTGTAATCAGTATCTTTGTTGCTGGCCTTTTATTGGTTTCTCAAGATTTGCACAATGTGTTTGTGAAAATTTCTGAAGCGTTATTTTCAACTTTTACTTCACTGCTGGTGGCTTCCCGTATTTTTGTTTTTGGGTTATTGTTGTCACATCGTATAAAGTGGTGGGGGAGAATGTTTGATGAAGGTAATGCCAATTGTCATGGTAATTCAACAGTGATTTTCGAGGGAATGGTGGAAAAGAGATTATTTTTTCTCTTTTGTTTATTTTTTCCTCTAGGAACTTGACTGAAATCGTTTCTTCTGTATTGCTGAATCAATGATTTACATATTTGTCAATCAATTTCGGGAAAATCATAAGCTACATTTGGGAGGGAAAGTGTTGAGAAAAAGATGGAGTCGTTTGAGAGTTTCCTCCTGCATACGCATAGATTGAAAGTTCATCTGCTTTTATTTTCTTATTTTTCCCTTGGACTTTATGGAGATTAGCTTGCATTTTATTGTTATGATGAAGATCTTGTGACTTGGTTTCACGCTCTTATGGAGTATACATACATGGACGAAAAGAATAGGCAATTTGTTATATCTTTACTTTCTTGCTCTGAAAATTATAGCATATTCTGACGTATATTATGAGATTAGTACTATATTAATTGGTTGCCATTTATTCTCCAATTCCTTTTCACTGAATGGCACTAAGGAATCACTTGCCTTGCGAGTTTGGTAAGAAGATTATGAATTATTTTGTTTTTCTGCGTTGTTTGTCTTTTGTTAGTGCTATTATATGAGTCAATATTCTCTTTCCAACCATGACTGAAAGAAATTAAGCTTACATTGACATGATCATGTATATGACTTTATAGTTATTATAGGCAATCATATTTGAAGTATATGAATTCACGTTATTTTATTGCCATGTGTAACTTGTTTATATTTTTCTATCCAGTTTTGGCGTTCAAAGCCCTGGTACGTAGAGATGCAGAGAAAATGAGCTGTTAGGTGATGGTTGAACAGGTCTAGAACTGTTCTGTAGGTTTCCTAAATGATGACGAATGATGAATTTGTTCAACATTCTGATCATGTCTTAACGAGGAGAAGCTGAACTTAATCATTCTAGTAGTTAAATTTCAGAACTTTCAAAGGTTAGAATGGTTAAGAAACTGGGGATGAAGAAAGCTTCATCCAGTTTAAATAATCGGTCTGCTAGTAGGAAGTCGTACAGGAAGATAGATAACCCGGCTCGAGTTCCAACAGCTCCTGGGAAATCTATACAGTCTGGAATCTCAAGTACATGGGTATGCAAAAATTCTGCTTGTAGGGCTGTTTTGTCAGTAGATGATACATTTTGCAAGAGATGCTCTTGCTGTATCTGTCACTTATTTGATGACAACAAGGACCCTAGTCTCTGGCTGGTGTGCTCTACTGAATCTGGACAGAGAGATTCCTGTGGATTATCTTGCCATATTGAGTGTGCCTTGCAGCGTGAGAAGGTGGGGGTTGTTGATCTTGGTCAATTAATGCAGCTAGATGGTAGTTATTGCTGTGCTTCTTGTGGCAAAGTTTCTGGGATACTAGAGTAAGTAATTTTCTTGTTCAATATTTCCTTTAATTCTCATATTTTGCATAATTGTGGGCTCTCAAGCCGCGGAGAGGTGTTGTTGCATCGTTTATATTTATTCCAGCAATCTTATTCTAGCTATGGCCTGATTGGATTGTGAAATTCTTTACTGGCAGATGTTGGAAGAAGCAACTAGCCATAGCTAGAGATGCACGCCGTGTTGATGTTCTCTGTTACAGGATTTATTTAGGTTATAGGCTCCTTGAAAGGACTTCAAGGTTTAAGGAACTGCACGAAATTATTCAAGATGCAAAGGCCAAGCTAGAAACAGAAGTGGGTCCAGTAAATGGGATTTCCGCCAAGATGGCTCGAGGTATTGTCTGCAGGCTATCTGTTGGCGGTGATGTGCAGAAGCTTTGCTCATTCGCAATTGAAAAAGCAGATCAATGGCTCGCTGGAGTGTCTAATACAAATCTGAACTGCAGAGGTTTAATCCATGACCATTATGTTTCTTGCTCCAACTATTGTCTATAAATCTCATTAGCTTTTCTGTTGCTTATATCTTTTCCCATAATTTCATTTTTGACAGACGATTCATTTCCGGCTGCTTGCAAGCTTCTATTTGAGGAGATAAAATATTCTTCCATTGTGATAACTTTAGTTGAAATCTCGAATTCATCGTCTAATGAGACTAAGGGCTACAAGCTTTGGTATAGTAAAAGTAGAGAAGAAACATACACAGAAGAACCTATTTGTGTATTTCCTAGAGCTCAGAGAAGGATTTTGATATCCAATCTACAACCTTGCACCGAATACATGTTCAGAATTGTTTCATATTCAGAGAATGGTGACGTTGGTCACTCTGAGGCCAAGTGTTTTACCAAGAGCGTGGAAATAATTCACAACTCTCATTCTCCCGCCCCTTCAAATCACAGGAAAGAAAGTCCTGTTACTGAAGAAAGCTGTATCCGCAAGAAGGGTCCAGATGATACAACCATTATGTGTTCGTCCTCAGGATTTCAAGTACGAGATCTTGGAAAGATTCTGGAACTTGCTAGGGCTCAAGGAGAAGGTTGTCTTGAGAGCCTTTGCTGTGCTGATGTAAAAAATTGTTGTAGAGTGCAGATGGGTGTCAAGCCAGAAACTCCGGAAGAAGAGCAGCTTCCTCCTGTTTCTCGTGGACTTGATTTAAATGTGGTTTCAGTACCTGATCTGAATGAAGAACTAACTCCTCCTTTTGAGTGTTTTAGGGATGAAGTTAATGGCTGCACTCTGCAGCAGGCTGTTGAGGCAGATGAAGATGCTGCCTCCCATGACATAGAGAAAAATGGCTTGGCAAGATCACACGGTAGTGATGATTCTCAGATCTGGACTTGTGGACCAAATGGTGAGGTGCCGGCTGTTGATTCCCTTACAGGGTTGTACAGGAAAAGGGCAGCTAGCACAAGCGAAGATCCGAATGACTGTGACAGCACTTTAATAAATGGATCGCCGCTCCCATTATCAAATGGTTCATGTTTCTTGGACGAGAACTTCGAGTATTGTGTTAAGATAATTCGATGGCTAGAATGTGAAGGTCACATTAAACAGGAATTTAGATTGAAACTTCTAACATGGTTTAGCTTGAGATCAACAGAGCAAGAACGTAGGGTAGTCAGCACCTTTATCCAAACACTCATTGATGAACCCAGTAGCTTGGCTGGACAGTTAGTTGACTCCTTTTCTGATATCATATCCAGCAAGAAGCCACGAAATGGGTTCTGTAGTAAGCTTTGGCATTAGATTAGCATACTCATGATGCAGAAATCACCGTTAACTCGTACTTATCTTACCTTACCATTTCTCTGCTACTCTCTCCTCTAATTAGTCTGCCGTTATTTGTACAGGCATTTTTTTCTTGATCACTGTCATCAAGTGATTCATTTATATCTCGGACAATGTCATCCGGGTTATGTTAAAGGAATTGGATGCTGATTGCAGAGACAGATTACTCAATTTTTCATAGAATGTAACAATTGGTGGTGGCCGGTACC

mRNA sequence

CGAAGACTCTTTCTCTGTCTCTCTGTCTCTCTCTCTCTCTTCTTCTTCTTTCTCTCTCTAGCTTCATGCTTGCGCAGAACAAATTATTCGGTTTCGGCGCATCGAAGCCCTAGATTCGTCCCCATTCTTCGCCGGTACGTCCCGAATATTTCTCTTTTATTTATATCGTTTCATTCAAACGATTTCCTTGGTTCTTTGGGGCTGTTTCTGTGGCAGATCCATTGATTTTCGAGCTTCCTTCTTGGTTTCCTAAATGATGACGAATGATGAATTTGTTCAACATTCTGATCATGTCTTAACGAGGAGAAGCTGAACTTAATCATTCTAGTAGTTAAATTTCAGAACTTTCAAAGGTTAGAATGGTTAAGAAACTGGGGATGAAGAAAGCTTCATCCAGTTTAAATAATCGGTCTGCTAGTAGGAAGTCGTACAGGAAGATAGATAACCCGGCTCGAGTTCCAACAGCTCCTGGGAAATCTATACAGTCTGGAATCTCAAGTACATGGGTATGCAAAAATTCTGCTTGTAGGGCTGTTTTGTCAGTAGATGATACATTTTGCAAGAGATGCTCTTGCTGTATCTGTCACTTATTTGATGACAACAAGGACCCTAGTCTCTGGCTGGTGTGCTCTACTGAATCTGGACAGAGAGATTCCTGTGGATTATCTTGCCATATTGAGTGTGCCTTGCAGCGTGAGAAGGTGGGGGTTGTTGATCTTGGTCAATTAATGCAGCTAGATGGTAGTTATTGCTGTGCTTCTTGTGGCAAAGTTTCTGGGATACTAGAATGTTGGAAGAAGCAACTAGCCATAGCTAGAGATGCACGCCGTGTTGATGTTCTCTGTTACAGGATTTATTTAGGTTATAGGCTCCTTGAAAGGACTTCAAGGTTTAAGGAACTGCACGAAATTATTCAAGATGCAAAGGCCAAGCTAGAAACAGAAGTGGGTCCAGTAAATGGGATTTCCGCCAAGATGGCTCGAGGTATTGTCTGCAGGCTATCTGTTGGCGGTGATGTGCAGAAGCTTTGCTCATTCGCAATTGAAAAAGCAGATCAATGGCTCGCTGGAGTGTCTAATACAAATCTGAACTGCAGAGACGATTCATTTCCGGCTGCTTGCAAGCTTCTATTTGAGGAGATAAAATATTCTTCCATTGTGATAACTTTAGTTGAAATCTCGAATTCATCGTCTAATGAGACTAAGGGCTACAAGCTTTGGTATAGTAAAAGTAGAGAAGAAACATACACAGAAGAACCTATTTGTGTATTTCCTAGAGCTCAGAGAAGGATTTTGATATCCAATCTACAACCTTGCACCGAATACATGTTCAGAATTGTTTCATATTCAGAGAATGGTGACGTTGGTCACTCTGAGGCCAAGTGTTTTACCAAGAGCGTGGAAATAATTCACAACTCTCATTCTCCCGCCCCTTCAAATCACAGGAAAGAAAGTCCTGTTACTGAAGAAAGCTGTATCCGCAAGAAGGGTCCAGATGATACAACCATTATGTGTTCGTCCTCAGGATTTCAAGTACGAGATCTTGGAAAGATTCTGGAACTTGCTAGGGCTCAAGGAGAAGGTTGTCTTGAGAGCCTTTGCTGTGCTGATGTAAAAAATTGTTGTAGAGTGCAGATGGGTGTCAAGCCAGAAACTCCGGAAGAAGAGCAGCTTCCTCCTGTTTCTCGTGGACTTGATTTAAATGTGGTTTCAGTACCTGATCTGAATGAAGAACTAACTCCTCCTTTTGAGTGTTTTAGGGATGAAGTTAATGGCTGCACTCTGCAGCAGGCTGTTGAGGCAGATGAAGATGCTGCCTCCCATGACATAGAGAAAAATGGCTTGGCAAGATCACACGGTAGTGATGATTCTCAGATCTGGACTTGTGGACCAAATGGTGAGGTGCCGGCTGTTGATTCCCTTACAGGGTTGTACAGGAAAAGGGCAGCTAGCACAAGCGAAGATCCGAATGACTGTGACAGCACTTTAATAAATGGATCGCCGCTCCCATTATCAAATGGTTCATGTTTCTTGGACGAGAACTTCGAGTATTGTGTTAAGATAATTCGATGGCTAGAATGTGAAGGTCACATTAAACAGGAATTTAGATTGAAACTTCTAACATGGTTTAGCTTGAGATCAACAGAGCAAGAACGTAGGGTAGTCAGCACCTTTATCCAAACACTCATTGATGAACCCAGTAGCTTGGCTGGACAGTTAGTTGACTCCTTTTCTGATATCATATCCAGCAAGAAGCCACGAAATGGGTTCTGTAGTAAGCTTTGGCATTAGATTAGCATACTCATGATGCAGAAATCACCGTTAACTCGTACTTATCTTACCTTACCATTTCTCTGCTACTCTCTCCTCTAATTAGTCTGCCGTTATTTGTACAGGCATTTTTTTCTTGATCACTGTCATCAAGTGATTCATTTATATCTCGGACAATGTCATCCGGGTTATGTTAAAGGAATTGGATGCTGATTGCAGAGACAGATTACTCAATTTTTCATAGAATGTAACAATTGGTGGTGGCCGGTACC

Coding sequence (CDS)

ATGGTTAAGAAACTGGGGATGAAGAAAGCTTCATCCAGTTTAAATAATCGGTCTGCTAGTAGGAAGTCGTACAGGAAGATAGATAACCCGGCTCGAGTTCCAACAGCTCCTGGGAAATCTATACAGTCTGGAATCTCAAGTACATGGGTATGCAAAAATTCTGCTTGTAGGGCTGTTTTGTCAGTAGATGATACATTTTGCAAGAGATGCTCTTGCTGTATCTGTCACTTATTTGATGACAACAAGGACCCTAGTCTCTGGCTGGTGTGCTCTACTGAATCTGGACAGAGAGATTCCTGTGGATTATCTTGCCATATTGAGTGTGCCTTGCAGCGTGAGAAGGTGGGGGTTGTTGATCTTGGTCAATTAATGCAGCTAGATGGTAGTTATTGCTGTGCTTCTTGTGGCAAAGTTTCTGGGATACTAGAATGTTGGAAGAAGCAACTAGCCATAGCTAGAGATGCACGCCGTGTTGATGTTCTCTGTTACAGGATTTATTTAGGTTATAGGCTCCTTGAAAGGACTTCAAGGTTTAAGGAACTGCACGAAATTATTCAAGATGCAAAGGCCAAGCTAGAAACAGAAGTGGGTCCAGTAAATGGGATTTCCGCCAAGATGGCTCGAGGTATTGTCTGCAGGCTATCTGTTGGCGGTGATGTGCAGAAGCTTTGCTCATTCGCAATTGAAAAAGCAGATCAATGGCTCGCTGGAGTGTCTAATACAAATCTGAACTGCAGAGACGATTCATTTCCGGCTGCTTGCAAGCTTCTATTTGAGGAGATAAAATATTCTTCCATTGTGATAACTTTAGTTGAAATCTCGAATTCATCGTCTAATGAGACTAAGGGCTACAAGCTTTGGTATAGTAAAAGTAGAGAAGAAACATACACAGAAGAACCTATTTGTGTATTTCCTAGAGCTCAGAGAAGGATTTTGATATCCAATCTACAACCTTGCACCGAATACATGTTCAGAATTGTTTCATATTCAGAGAATGGTGACGTTGGTCACTCTGAGGCCAAGTGTTTTACCAAGAGCGTGGAAATAATTCACAACTCTCATTCTCCCGCCCCTTCAAATCACAGGAAAGAAAGTCCTGTTACTGAAGAAAGCTGTATCCGCAAGAAGGGTCCAGATGATACAACCATTATGTGTTCGTCCTCAGGATTTCAAGTACGAGATCTTGGAAAGATTCTGGAACTTGCTAGGGCTCAAGGAGAAGGTTGTCTTGAGAGCCTTTGCTGTGCTGATGTAAAAAATTGTTGTAGAGTGCAGATGGGTGTCAAGCCAGAAACTCCGGAAGAAGAGCAGCTTCCTCCTGTTTCTCGTGGACTTGATTTAAATGTGGTTTCAGTACCTGATCTGAATGAAGAACTAACTCCTCCTTTTGAGTGTTTTAGGGATGAAGTTAATGGCTGCACTCTGCAGCAGGCTGTTGAGGCAGATGAAGATGCTGCCTCCCATGACATAGAGAAAAATGGCTTGGCAAGATCACACGGTAGTGATGATTCTCAGATCTGGACTTGTGGACCAAATGGTGAGGTGCCGGCTGTTGATTCCCTTACAGGGTTGTACAGGAAAAGGGCAGCTAGCACAAGCGAAGATCCGAATGACTGTGACAGCACTTTAATAAATGGATCGCCGCTCCCATTATCAAATGGTTCATGTTTCTTGGACGAGAACTTCGAGTATTGTGTTAAGATAATTCGATGGCTAGAATGTGAAGGTCACATTAAACAGGAATTTAGATTGAAACTTCTAACATGGTTTAGCTTGAGATCAACAGAGCAAGAACGTAGGGTAGTCAGCACCTTTATCCAAACACTCATTGATGAACCCAGTAGCTTGGCTGGACAGTTAGTTGACTCCTTTTCTGATATCATATCCAGCAAGAAGCCACGAAATGGGTTCTGTAGTAAGCTTTGGCATTAG

Protein sequence

MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDLGQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKELHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEPICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Homology
BLAST of Carg12181-RA vs. NCBI nr
Match: KAG7021834.1 (VIN3-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 643/643 (100.00%), Postives = 643/643 (100.00%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL
Sbjct: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL
Sbjct: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE
Sbjct: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN
Sbjct: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
           TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP
Sbjct: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN
Sbjct: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
           HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN
Sbjct: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
           CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE
Sbjct: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD
Sbjct: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR
Sbjct: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Sbjct: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 643

BLAST of Carg12181-RA vs. NCBI nr
Match: XP_022932185.1 (VIN3-like protein 1 [Cucurbita moschata])

HSP 1 Score: 1308.1 bits (3384), Expect = 0.0e+00
Identity = 640/643 (99.53%), Postives = 643/643 (100.00%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL
Sbjct: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL
Sbjct: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE
Sbjct: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN
Sbjct: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
           TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP
Sbjct: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN
Sbjct: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
           HRKESPVTEESCIRKKGPDDTTI+CSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN
Sbjct: 361 HRKESPVTEESCIRKKGPDDTTIICSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
           CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPF+CFRDEVNGCTLQQAVE
Sbjct: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFDCFRDEVNGCTLQQAVE 480

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKR+ASTSEDPNDCD
Sbjct: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRSASTSEDPNDCD 540

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR
Sbjct: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Sbjct: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 643

BLAST of Carg12181-RA vs. NCBI nr
Match: KAG6587945.1 (VIN3-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1307.0 bits (3381), Expect = 0.0e+00
Identity = 640/643 (99.53%), Postives = 642/643 (99.84%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           MVKKLGMKKASSSLNNRSASRK YRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL
Sbjct: 207 MVKKLGMKKASSSLNNRSASRKLYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 266

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL
Sbjct: 267 SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 326

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE
Sbjct: 327 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 386

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN
Sbjct: 387 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 446

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
           TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP
Sbjct: 447 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 506

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPRAQRRILISNLQPCTEYMFRIVSY+ENGDVGHSEAKCFTKSVEIIHNSHSPAPSN
Sbjct: 507 ICVFPRAQRRILISNLQPCTEYMFRIVSYTENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 566

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
           HRKESPVTEESCIRKKGPDDTTI+CSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN
Sbjct: 567 HRKESPVTEESCIRKKGPDDTTIICSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 626

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
           CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE
Sbjct: 627 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 686

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD
Sbjct: 687 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 746

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR
Sbjct: 747 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 806

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Sbjct: 807 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 849

BLAST of Carg12181-RA vs. NCBI nr
Match: XP_023530610.1 (VIN3-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1290.8 bits (3339), Expect = 0.0e+00
Identity = 632/643 (98.29%), Postives = 638/643 (99.22%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           MVKKLGM KASSSLNNRSASRKSYRKIDNPARVPTAP KS+QSGISSTWVCKNSACRAVL
Sbjct: 1   MVKKLGMMKASSSLNNRSASRKSYRKIDNPARVPTAPEKSLQSGISSTWVCKNSACRAVL 60

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL
Sbjct: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE
Sbjct: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFA+EKADQWLAGVSN
Sbjct: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAVEKADQWLAGVSN 240

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
           TNLNCRDDSFPAACKLLFEEIK SSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP
Sbjct: 241 TNLNCRDDSFPAACKLLFEEIKSSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPR QRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFT+SVEIIHNSHSPAPSN
Sbjct: 301 ICVFPRDQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTQSVEIIHNSHSPAPSN 360

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
           HRKESPVTEESCIRKKGPDDT+I+CSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN
Sbjct: 361 HRKESPVTEESCIRKKGPDDTSIICSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
           CCRVQ+GVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDE NGCTLQQAVE
Sbjct: 421 CCRVQIGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEGNGCTLQQAVE 480

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD
Sbjct: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR
Sbjct: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Sbjct: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 643

BLAST of Carg12181-RA vs. NCBI nr
Match: XP_023002374.1 (VIN3-like protein 1 [Cucurbita maxima])

HSP 1 Score: 1279.6 bits (3310), Expect = 0.0e+00
Identity = 628/643 (97.67%), Postives = 633/643 (98.44%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           MVKKLGMKKASSSLNNRSASRK YRKID PARV TAP KS+QSGISSTWVCKNSACRAVL
Sbjct: 1   MVKKLGMKKASSSLNNRSASRKQYRKIDKPARVSTAPEKSLQSGISSTWVCKNSACRAVL 60

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFCKRCSCCICHLFDDNKDPSLWLVCS ESGQRDSCGLSCHIECALQREKVGVVDL
Sbjct: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSAESGQRDSCGLSCHIECALQREKVGVVDL 120

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE
Sbjct: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN
Sbjct: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
           TNLNCRDDSFPAACKLLFEEIK SSIVITLVEISN+SSNETKGYKLWYSKSREE YTEEP
Sbjct: 241 TNLNCRDDSFPAACKLLFEEIKSSSIVITLVEISNTSSNETKGYKLWYSKSREEPYTEEP 300

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGD+GHSEAKCFTKSVEIIHNSHSPAPSN
Sbjct: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDIGHSEAKCFTKSVEIIHNSHSPAPSN 360

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
           HRKESPV EESCIRKKGPDDTTI+CSSSGFQVRDLGKILELARAQGEG LESLCCADVKN
Sbjct: 361 HRKESPVAEESCIRKKGPDDTTIICSSSGFQVRDLGKILELARAQGEGRLESLCCADVKN 420

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
           CCRVQ+GVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDE NGCTLQQAVE
Sbjct: 421 CCRVQIGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEGNGCTLQQAVE 480

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD
Sbjct: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR
Sbjct: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Sbjct: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 643

BLAST of Carg12181-RA vs. ExPASy Swiss-Prot
Match: Q9LHF5 (VIN3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=VIL1 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.6e-144
Identity = 297/630 (47.14%), Postives = 391/630 (62.06%), Query Frame = 0

Query: 15  NNRSASRKSYRKIDNPARVPTAPGKSIQSG-ISSTWVCKNSACRAVLSVDDTFCKRCSCC 74
           ++R  ++KS +K ++  +           G + S+W+CKN++CRA +  +D+FCKRCSCC
Sbjct: 12  HSRKTNKKSNKKHESNGKQQQQQDVDGGGGCLRSSWICKNASCRANVPKEDSFCKRCSCC 71

Query: 75  ICHLFDDNKDPSLWLVCSTE-SGQRDSCGLSCHIECALQREKVGVVDLGQLMQLDGSYCC 134
           +CH FD+NKDPSLWLVC  E S   + CGLSCHIECA +  KVGV+ LG LM+LDG +CC
Sbjct: 72  VCHNFDENKDPSLWLVCEPEKSDDVEFCGLSCHIECAFREVKVGVIALGNLMKLDGCFCC 131

Query: 135 ASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKELHEIIQDAKAKL 194
            SCGKVS IL CWKKQL  A++ARR D LCYRI LGYRLL  TSRF ELHEI++ AK+ L
Sbjct: 132 YSCGKVSQILGCWKKQLVAAKEARRRDGLCYRIDLGYRLLNGTSRFSELHEIVRAAKSML 191

Query: 195 ETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSNTNLNCRDDSFPA 254
           E EVGP++G +A+  RGIV RL V  +VQ+LC+ AI+KA +  A       N   D  PA
Sbjct: 192 EDEVGPLDGPTARTDRGIVSRLPVAANVQELCTSAIKKAGELSA-------NAGRDLVPA 251

Query: 255 ACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEPICV-FPRAQRRI 314
           AC+  FE+I    + + L+E+ ++   + KGYKLWY K  E    E+ + V   R +RR+
Sbjct: 252 ACRFHFEDIAPKQVTLRLIELPSAVEYDVKGYKLWYFKKGE--MPEDDLFVDCSRTERRM 311

Query: 315 LISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSNHRKESPVTEES 374
           +IS+L+PCTEY FR+VSY+E G  GHS A CFTKSVEI+       P + +++  +    
Sbjct: 312 VISDLEPCTEYTFRVVSYTEAGIFGHSNAMCFTKSVEIL------KPVDGKEKRTI---D 371

Query: 375 CIRKKGPDDTTIMCS-SSGFQVRDLGKILELARAQGEGCLESLCCADVKNCCRVQMGVKP 434
            +    P D     S SS FQ+  LGK ++LA AQ EG LE+    D +  C        
Sbjct: 372 LVGNAQPSDREEKSSISSRFQIGQLGKYVQLAEAQEEGLLEAFYNVDTEKIC-------- 431

Query: 435 ETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVEA---DEDAAS 494
           E PEEE  P    G DLNVVSVPDLNEE TPP +    E NG  L    EA   D D   
Sbjct: 432 EPPEEELPPRRPHGFDLNVVSVPDLNEEFTPP-DSSGGEDNGVPLNSLAEADGGDHDDNC 491

Query: 495 HDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPN-DCDSTLING 554
            D   NG  R   ++D  + + G +G+    D L    RK  + +++  N +CDS+ I  
Sbjct: 492 DDAVSNG--RRKNNNDCLVISDG-SGDDTGFDFLMTRKRKAISDSNDSENHECDSSSI-- 551

Query: 555 SPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERRVVSTFI 614
                       D+  E CVK+IRWLE EGHIK  FR++ LTWFS+ ST QE+ VVSTF+
Sbjct: 552 ------------DDTLEKCVKVIRWLEREGHIKTTFRVRFLTWFSMSSTAQEQSVVSTFV 597

Query: 615 QTLIDEPSSLAGQLVDSFSDIISSKKPRNG 637
           QTL D+P SLAGQLVD+F+D++S+K+P NG
Sbjct: 612 QTLEDDPGSLAGQLVDAFTDVVSTKRPNNG 597

BLAST of Carg12181-RA vs. ExPASy Swiss-Prot
Match: Q9SUM4 (VIN3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=VIL2 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 4.3e-89
Identity = 226/683 (33.09%), Postives = 329/683 (48.17%), Query Frame = 0

Query: 8   KKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQS-------------GISSTWVCKNS 67
           KK  S       + K  RK+DNP+R        + S             G S+T  CKN 
Sbjct: 90  KKRDSDCLPIQRNTKRQRKVDNPSRYVIPATNIVTSNNASGSCSSVNTKGESTTIYCKNL 149

Query: 68  ACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESG-QRDSCGLSCHIECALQRE 127
           ACRAVL  +D+FC+RCSCCIC  +DDNKDPSLWL CS++   + +SCG SCH+ECA   E
Sbjct: 150 ACRAVLRQEDSFCRRCSCCICRKYDDNKDPSLWLTCSSDPPFEGESCGFSCHLECAFNTE 209

Query: 128 KVGVVDLGQLMQLDG-SYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLL 187
           K G   LG+  Q +G  + C SCGK + +LECWKKQL IA++ RRV+VLCYR++L  +LL
Sbjct: 210 KSG---LGKDKQSEGCCFYCVSCGKANSLLECWKKQLTIAKETRRVEVLCYRLFLVQKLL 269

Query: 188 ERTSRFKELHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIE--- 247
           + +++++ L E++ +A   LE +VGP+ G+  KM RGIV RL  G DVQKLCS A+E   
Sbjct: 270 KSSTKYRNLCEVVDEAVKTLEADVGPLTGLPMKMGRGIVNRLHSGPDVQKLCSSALESLE 329

Query: 248 ------------------KADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITLV 307
                             K  Q  + V +  ++  D +   + K+ FE++  +S+ + L 
Sbjct: 330 TIATTPPDVAALPSPRSSKMQQDCSYVLSNEISA-DTATTGSTKIRFEDVNATSLTVVLA 389

Query: 308 EISNSSSNETKGYKLWYSKSREETYTEEPICVFPRAQRRILISNLQPCTEYMFRIVSYSE 367
                S      Y +W+ K  E+ Y E+  C       R ++S L P +EY F++VSYS 
Sbjct: 390 SNEIPSPPNIVHYSIWHRKVPEKDYPEKSTCTLFIPNTRFVVSGLAPASEYCFKVVSYSG 449

Query: 368 NGDVGHSEAKCFTKSVEIIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQ 427
             ++G  E    T+S E   N  S      R  SP+T  +C        +T+  + S  +
Sbjct: 450 TREMGVDEINVLTRSAEEGANCSSAV---ERSVSPLT--NC--------STLSSNPSSVE 509

Query: 428 VRDLGKILELARAQGEGCLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVS 487
                  +                   K   + +    P   +E     + R  D ++V 
Sbjct: 510 AESNNDYI----------------VPKKPSSKNEDNNSPSV-DESAAKRMKRTTDSDIVQ 569

Query: 488 VPDLNEELTPPFECFRDEVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGP 547
           +    E++        DE     L +     E      +    L  +  S D+ +    P
Sbjct: 570 IEKDVEQIV----LLDDEEQEAVLDKT----ESETPVVVTTKSLVGNRNSSDASL----P 629

Query: 548 NGEVPAVDSLTGLYRKRAASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRW 607
                + D +     +   S  ++ N+ D +   G+           +   E+CVKIIR 
Sbjct: 630 ITPFRS-DEIKNRQARIEISMKDNCNNGDHSANGGT-----------ESGLEHCVKIIRQ 689

Query: 608 LECEGHIKQEFRLKLLTWFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSK 644
           LEC GHI + FR K LTW+SLR+T QE RVV  FI T ID+P +LA QL+D+F D +S K
Sbjct: 690 LECSGHIDKNFRQKFLTWYSLRATSQEIRVVKIFIDTFIDDPMALAEQLIDTFDDRVSIK 714

BLAST of Carg12181-RA vs. ExPASy Swiss-Prot
Match: Q9FIE3 (Protein VERNALIZATION INSENSITIVE 3 OS=Arabidopsis thaliana OX=3702 GN=VIN3 PE=1 SV=2)

HSP 1 Score: 297.0 bits (759), Expect = 5.2e-79
Identity = 210/601 (34.94%), Postives = 307/601 (51.08%), Query Frame = 0

Query: 51  CKNSACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECAL 110
           C+N ACRA L  DDTFC+RCSCCIC  FDDNKDPSLWL C       D+CG SCH+EC L
Sbjct: 130 CENLACRAALGCDDTFCRRCSCCICQKFDDNKDPSLWLTC-------DACGSSCHLECGL 189

Query: 111 QREKVGVVDLGQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYR 170
           ++++ G+        LDG + CA CGK + +L CW+KQ+ +A++ RRVDVLCYR+ LG +
Sbjct: 190 KQDRYGIGS----DDLDGRFYCAYCGKDNDLLGCWRKQVKVAKETRRVDVLCYRLSLGQK 249

Query: 171 LLERTSRFKELHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEK 230
           LL  T++++ L E++ +A  KLE +VGP++G + KMARGIV RLS G  VQKLCS A+E 
Sbjct: 250 LLRGTTKYRNLLELMDEAVKKLEGDVGPLSGWAMKMARGIVNRLSSGVHVQKLCSQAMEA 309

Query: 231 ADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITL--VEISNSSSNETKGYKLWY 290
            D+ ++   + +++ + D       +  EEI+  S+ + +   E S+S+ N+  G++L+ 
Sbjct: 310 LDKVVS--PSESVSGQGDKM----TVRVEEIQARSVTVRVDSEEPSSSTQNKITGFRLFC 369

Query: 291 SKSREETYTEEPICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVE 350
            KS++E  + +  CV    +    I  L+P TE+  R+VS++E GD+  SE +  T    
Sbjct: 370 RKSKDEECSSQGNCVVYLPETTSAIQGLEPDTEFCLRVVSFNEEGDLDESELRFTTLK-- 429

Query: 351 IIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEG 410
                         ++SP+T  S          + +CS+      D    +  + ++G G
Sbjct: 430 ------DDGDEAGDQQSPLTNSS----------SGLCSNPSLP-EDESNNVNKSCSKGNG 489

Query: 411 CLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRD 470
                   D  N      G      EEE+L  V R    N +   DL   L  P  C RD
Sbjct: 490 --------DKDNTEHCSAGEVESELEEERL--VKR--KANKIDGRDL---LVTP--CKRD 549

Query: 471 EVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKR 530
              G                  ++ G  R                           ++ R
Sbjct: 550 IYKG------------------KQGGNKR---------------------------FKSR 609

Query: 531 AASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLT 590
             S +E P       IN +    +NG    D++  + VK IR LE EGHI + FR + LT
Sbjct: 610 TVSLNEKPE------INNA----ANG--VGDKDLGHIVKTIRCLEEEGHIDKSFRERFLT 620

Query: 591 WFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRN------GFCSKLW 644
           W+SLR+T +E RVV  F++T +++ SSL  QLVD+FS+ I SK+         G C KLW
Sbjct: 670 WYSLRATHREVRVVKIFVETFMEDLSSLGQQLVDTFSESILSKRSSTNGVVPAGICLKLW 620

BLAST of Carg12181-RA vs. ExPASy Swiss-Prot
Match: Q5BPT4 (VIN3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=VIL3 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.2e-51
Identity = 163/590 (27.63%), Postives = 241/590 (40.85%), Query Frame = 0

Query: 51  CKNSACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESG-QRDSCGLSCHIECA 110
           C+N AC+  L  + TFCKRCSCCIC  +DDNKDPSLWL C+++S    +SCGLSCH+ CA
Sbjct: 119 CQNLACQNKLREEATFCKRCSCCICFKYDDNKDPSLWLTCNSDSQFDGESCGLSCHLNCA 178

Query: 111 LQREKVGVVDLGQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGY 170
              EK G+ +      +DG + C SCGK +  +EC KKQL IA + RRV V CYRI L +
Sbjct: 179 FDSEKSGLKEDTPSSDIDGCFNCVSCGKTNSKIECLKKQLIIANEERRVGVFCYRILLAH 238

Query: 171 RLLERTSRFKELHEIIQDAKAKLETEVG-PVNGISAKMARGIVCRLSVGGDVQKLCSFAI 230
           +LL+ T ++  + E ++ A   L+ E G P++ + +KM+RG+V RL     V+K CS A+
Sbjct: 239 KLLKGTKKYIIVSEEVEKAVMHLKNEFGVPISCLPSKMSRGLVNRLCCAEKVKKHCSSAL 298

Query: 231 EKADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWY 290
           ++ D    G+          +   + K+  E +  +S+   +    + S  +T  Y++ Y
Sbjct: 299 KELD----GLP------LPSTIQGSMKIRIESVLATSVTFDIEAEESFSWGDTNHYRMVY 358

Query: 291 SKSREETYTEEPI--CVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKS 350
            K  E+  +++         + +R  +  L P TEY F+IVS+S                
Sbjct: 359 RKVSEKHSSKDLTRELFSTSSHQRFTVMELTPATEYWFKIVSFS---------------- 418

Query: 351 VEIIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQG 410
                                                                       
Sbjct: 419 ------------------------------------------------------------ 478

Query: 411 EGCLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECF 470
                               GV       E+L      +D  +VS   L +E        
Sbjct: 479 --------------------GV-------EEL-----SVDEFIVSTKTLQDE-------- 514

Query: 471 RDEVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYR 530
                                                          EV AV  L  +  
Sbjct: 539 -----------------------------------------------EVAAV--LLNMSN 514

Query: 531 KRAASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKL 590
              A+  E                  +GSC     FE CV +IR LEC G +K +FR K 
Sbjct: 599 CNNANKME-----------------KSGSCSF--GFEECVNLIRQLECSGQVKSDFRKKF 514

Query: 591 LTWFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNG 637
           LTW+ L++T++E+ VV  F+ T  D+  +LA QL+D+FSD I+ K P  G
Sbjct: 659 LTWYCLKATDKEKHVVEIFVDTFKDDKEALAKQLIDTFSDCITRKHPEIG 514

BLAST of Carg12181-RA vs. ExPASy Swiss-Prot
Match: Q94B71 (Protein OBERON 3 OS=Arabidopsis thaliana OX=3702 GN=OBE3 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 9.4e-04
Identity = 41/170 (24.12%), Postives = 68/170 (40.00%), Query Frame = 0

Query: 51  CKNSACRAVLSVDDT----------FCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSC 110
           C+N  C+++L VDD           FC  C C +C  FD   +   W+ C       D C
Sbjct: 408 CRNVNCKSLLPVDDCECKICSNNKGFCSSCMCPVCLRFDSASNTCSWVGC-------DVC 467

Query: 111 GLSCHIECALQRE--KVGVVDLGQLMQLDGSYCCASC-------GKVSGILECWKKQLAI 170
              CH  C +Q+   K G    GQ    +  + C  C       G V  +  C  K   +
Sbjct: 468 SHWCHAACGIQKNLIKPGHSLKGQRGTTEMMFHCIGCAHKSEMFGFVKDVFVCCAKNWGL 527

Query: 171 ARDARRVDVLCYRIYLGYRLLERTSRFKELHEIIQDAKAKLET-EVGPVN 201
               + +D +  +++ G       ++ K LH    +   KLE+ ++ P++
Sbjct: 528 ETLIKELDCV-RKVFRG----SDDAKGKALHLKANEMVKKLESKQISPLD 565

BLAST of Carg12181-RA vs. ExPASy TrEMBL
Match: A0A6J1F1I5 (VIN3-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438501 PE=4 SV=1)

HSP 1 Score: 1308.1 bits (3384), Expect = 0.0e+00
Identity = 640/643 (99.53%), Postives = 643/643 (100.00%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL
Sbjct: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL
Sbjct: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE
Sbjct: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN
Sbjct: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
           TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP
Sbjct: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN
Sbjct: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
           HRKESPVTEESCIRKKGPDDTTI+CSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN
Sbjct: 361 HRKESPVTEESCIRKKGPDDTTIICSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
           CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPF+CFRDEVNGCTLQQAVE
Sbjct: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFDCFRDEVNGCTLQQAVE 480

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKR+ASTSEDPNDCD
Sbjct: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRSASTSEDPNDCD 540

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR
Sbjct: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Sbjct: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 643

BLAST of Carg12181-RA vs. ExPASy TrEMBL
Match: A0A6J1KQA1 (VIN3-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496230 PE=4 SV=1)

HSP 1 Score: 1279.6 bits (3310), Expect = 0.0e+00
Identity = 628/643 (97.67%), Postives = 633/643 (98.44%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           MVKKLGMKKASSSLNNRSASRK YRKID PARV TAP KS+QSGISSTWVCKNSACRAVL
Sbjct: 1   MVKKLGMKKASSSLNNRSASRKQYRKIDKPARVSTAPEKSLQSGISSTWVCKNSACRAVL 60

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFCKRCSCCICHLFDDNKDPSLWLVCS ESGQRDSCGLSCHIECALQREKVGVVDL
Sbjct: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSAESGQRDSCGLSCHIECALQREKVGVVDL 120

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE
Sbjct: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN
Sbjct: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
           TNLNCRDDSFPAACKLLFEEIK SSIVITLVEISN+SSNETKGYKLWYSKSREE YTEEP
Sbjct: 241 TNLNCRDDSFPAACKLLFEEIKSSSIVITLVEISNTSSNETKGYKLWYSKSREEPYTEEP 300

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGD+GHSEAKCFTKSVEIIHNSHSPAPSN
Sbjct: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDIGHSEAKCFTKSVEIIHNSHSPAPSN 360

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
           HRKESPV EESCIRKKGPDDTTI+CSSSGFQVRDLGKILELARAQGEG LESLCCADVKN
Sbjct: 361 HRKESPVAEESCIRKKGPDDTTIICSSSGFQVRDLGKILELARAQGEGRLESLCCADVKN 420

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
           CCRVQ+GVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDE NGCTLQQAVE
Sbjct: 421 CCRVQIGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEGNGCTLQQAVE 480

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD
Sbjct: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR
Sbjct: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH
Sbjct: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 643

BLAST of Carg12181-RA vs. ExPASy TrEMBL
Match: A0A5D3BLD1 (VIN3-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G00360 PE=4 SV=1)

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 569/643 (88.49%), Postives = 598/643 (93.00%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           M+KK  MKK SSSLNNRSASRK +RKI+NP R+PTAP + + SGISSTWVCKNSACRAVL
Sbjct: 11  MIKKQEMKKTSSSLNNRSASRKQHRKIENPIRMPTAPEQCLHSGISSTWVCKNSACRAVL 70

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFC+RCSCCICHLFDDNKDPSLWLVCSTES Q DSCGLSCHIECALQR KVGVVDL
Sbjct: 71  SVDDTFCRRCSCCICHLFDDNKDPSLWLVCSTESEQGDSCGLSCHIECALQRGKVGVVDL 130

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQL IARDARRVD+LCYRIYL YRLLE TSRFKE
Sbjct: 131 GQLMQLDGSYCCASCGKVSGILECWKKQLVIARDARRVDILCYRIYLSYRLLETTSRFKE 190

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHE+IQ+AKAKLETEVGPVNGISAKMARGIVCRL+V GDVQKLCS AIEKADQWLA VSN
Sbjct: 191 LHEVIQEAKAKLETEVGPVNGISAKMARGIVCRLAVAGDVQKLCSLAIEKADQWLATVSN 250

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
            NLNCR+DS PAACK LFE IK SSIVITLVEI N+SS ETKGYKLWYSKSREE YT+EP
Sbjct: 251 PNLNCREDSLPAACKFLFEGIKSSSIVITLVEILNASSKETKGYKLWYSKSREEAYTKEP 310

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPR QRRILISNLQPCTEY FRIVSY+ENGD+GHSEAKCFTKSVEII+NSHSPAPSN
Sbjct: 311 ICVFPRTQRRILISNLQPCTEYTFRIVSYTENGDIGHSEAKCFTKSVEIINNSHSPAPSN 370

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
            RKESPV EESCIRK+GPD++ I+CSSSGFQVRDLGKIL+LAR QGEGCLE LC A+V N
Sbjct: 371 DRKESPVIEESCIRKRGPDNSFIICSSSGFQVRDLGKILQLARDQGEGCLERLCSANVAN 430

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
            C VQ GVKPETPEEEQLPPVS GLDLNVVSVPDLNEELTPPFE  RDEVNGCTLQQAVE
Sbjct: 431 GCGVQNGVKPETPEEEQLPPVSPGLDLNVVSVPDLNEELTPPFEYSRDEVNGCTLQQAVE 490

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGS DSQIWTCGPNGEVPAVDSLTGL RKRAAST+E+ NDCD
Sbjct: 491 ADEDAASHDIEKNGLARSHGSGDSQIWTCGPNGEVPAVDSLTGLCRKRAASTNEETNDCD 550

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPL ++NGSCFLDENFEYCVKIIRWLECEG+IKQEFRLKLLTWFSLRSTEQERR
Sbjct: 551 STLINGSPLRVANGSCFLDENFEYCVKIIRWLECEGYIKQEFRLKLLTWFSLRSTEQERR 610

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VV+TFIQTLIDEPSSLAGQLVDSFSDIIS KKPRNGFCSKLWH
Sbjct: 611 VVNTFIQTLIDEPSSLAGQLVDSFSDIISCKKPRNGFCSKLWH 653

BLAST of Carg12181-RA vs. ExPASy TrEMBL
Match: A0A1S3CJJ2 (VIN3-like protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501541 PE=4 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 566/643 (88.02%), Postives = 596/643 (92.69%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           M+KK  MKK SSSLNNRSASRK +RKI+NP R+PTAP + + SGISSTWVCKNSACRAVL
Sbjct: 11  MIKKQEMKKTSSSLNNRSASRKQHRKIENPIRMPTAPEQCLHSGISSTWVCKNSACRAVL 70

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFC+RCSCCICHLFDDNKDPSLWLVCSTES Q DSCGLSCHIECALQR KVGVVDL
Sbjct: 71  SVDDTFCRRCSCCICHLFDDNKDPSLWLVCSTESEQGDSCGLSCHIECALQRGKVGVVDL 130

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQL IARDARRVD+LCYRIYL YRLLE TSRFKE
Sbjct: 131 GQLMQLDGSYCCASCGKVSGILECWKKQLVIARDARRVDILCYRIYLSYRLLETTSRFKE 190

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHE+IQ+AKAKLETEVGPVNGISAKMARGIVCRL+V GDVQKLCS AIEKADQWLA VSN
Sbjct: 191 LHEVIQEAKAKLETEVGPVNGISAKMARGIVCRLAVAGDVQKLCSLAIEKADQWLATVSN 250

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
            NLNCR+DS PAACK LFE IK SSIVITLVEI N+SS ETKGYKLWYSKSREE YT+EP
Sbjct: 251 PNLNCREDSLPAACKFLFEGIKSSSIVITLVEIFNASSKETKGYKLWYSKSREEAYTKEP 310

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPR QRRILISNLQPCTEY FRIVSY+ENGD+GHSEAKCFTKSVEII+N HSPAPSN
Sbjct: 311 ICVFPRTQRRILISNLQPCTEYTFRIVSYTENGDIGHSEAKCFTKSVEIINNPHSPAPSN 370

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
            RKESPV EESCIRK+GPD++ I+CSSSGFQVRDLGKIL+LAR QG+GCLE LC A+V N
Sbjct: 371 DRKESPVIEESCIRKRGPDNSFIICSSSGFQVRDLGKILQLARDQGKGCLERLCSANVAN 430

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
            C VQ GVKPETPEEEQLPPVS GLDLNVVSVPDLNEELTPPFE  RDEVNGCTLQQAVE
Sbjct: 431 GCGVQNGVKPETPEEEQLPPVSPGLDLNVVSVPDLNEELTPPFEYSRDEVNGCTLQQAVE 490

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGS DSQIWTCGPNGEVPAVDSLTGL RKRA ST+E+ NDCD
Sbjct: 491 ADEDAASHDIEKNGLARSHGSGDSQIWTCGPNGEVPAVDSLTGLCRKRATSTNEETNDCD 550

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPL ++NGSCFLDENFEYCVKIIRWLECEG+IKQEFRLKLLTWFSLRSTEQERR
Sbjct: 551 STLINGSPLRVANGSCFLDENFEYCVKIIRWLECEGYIKQEFRLKLLTWFSLRSTEQERR 610

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VV+TFIQTLIDEPSSLAGQLVDSFSDIIS KKPRNGFCSKLWH
Sbjct: 611 VVNTFIQTLIDEPSSLAGQLVDSFSDIISCKKPRNGFCSKLWH 653

BLAST of Carg12181-RA vs. ExPASy TrEMBL
Match: A0A5A7TZG4 (VIN3-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001680 PE=4 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 566/643 (88.02%), Postives = 596/643 (92.69%), Query Frame = 0

Query: 1   MVKKLGMKKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQSGISSTWVCKNSACRAVL 60
           M+KK  MKK SSSLNNRSASRK +RKI+NP R+PTAP + + SGISSTWVCKNSACRAVL
Sbjct: 11  MIKKQEMKKTSSSLNNRSASRKQHRKIENPIRMPTAPEQCLHSGISSTWVCKNSACRAVL 70

Query: 61  SVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECALQREKVGVVDL 120
           SVDDTFC+RCSCCICHLFDDNKDPSLWLVCSTES Q DSCGLSCHIECALQR KVGVVDL
Sbjct: 71  SVDDTFCRRCSCCICHLFDDNKDPSLWLVCSTESEQGDSCGLSCHIECALQRGKVGVVDL 130

Query: 121 GQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKE 180
           GQLMQLDGSYCCASCGKVSGILECWKKQL IARDARRVD+LCYRIYL YRLLE TSRFKE
Sbjct: 131 GQLMQLDGSYCCASCGKVSGILECWKKQLVIARDARRVDILCYRIYLSYRLLETTSRFKE 190

Query: 181 LHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSN 240
           LHE+IQ+AKAKLETEVGPVNGISAKMARGIVCRL+V GDVQKLCS AIEKADQWLA VSN
Sbjct: 191 LHEVIQEAKAKLETEVGPVNGISAKMARGIVCRLAVAGDVQKLCSLAIEKADQWLATVSN 250

Query: 241 TNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEP 300
            NLNCR+DS PAACK LFE IK SSIVITLVEI N+SS ETKGYKLWYSKSREE YT+EP
Sbjct: 251 PNLNCREDSLPAACKFLFEGIKSSSIVITLVEIFNASSKETKGYKLWYSKSREEAYTKEP 310

Query: 301 ICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSN 360
           ICVFPR QRRILISNLQPCTEY FRIVSY+ENGD+GHSEAKCFTKSVEII+N HSPAPSN
Sbjct: 311 ICVFPRTQRRILISNLQPCTEYTFRIVSYTENGDIGHSEAKCFTKSVEIINNPHSPAPSN 370

Query: 361 HRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEGCLESLCCADVKN 420
            RKESPV EESCIRK+GPD++ I+CSSSGFQVRDLGKIL+LAR QG+GCLE LC A+V N
Sbjct: 371 DRKESPVIEESCIRKRGPDNSFIICSSSGFQVRDLGKILQLARDQGKGCLERLCSANVAN 430

Query: 421 CCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVE 480
            C VQ GVKPETPEEEQLPPVS GLDLNVVSVPDLNEELTPPFE  RDEVNGCTLQQAVE
Sbjct: 431 GCGVQNGVKPETPEEEQLPPVSPGLDLNVVSVPDLNEELTPPFEYSRDEVNGCTLQQAVE 490

Query: 481 ADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPNDCD 540
           ADEDAASHDIEKNGLARSHGS DSQIWTCGPNGEVPAVDSLTGL RKRA ST+E+ NDCD
Sbjct: 491 ADEDAASHDIEKNGLARSHGSGDSQIWTCGPNGEVPAVDSLTGLCRKRATSTNEETNDCD 550

Query: 541 STLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERR 600
           STLINGSPL ++NGSCFLDENFEYCVKIIRWLECEG+IKQEFRLKLLTWFSLRSTEQERR
Sbjct: 551 STLINGSPLRVANGSCFLDENFEYCVKIIRWLECEGYIKQEFRLKLLTWFSLRSTEQERR 610

Query: 601 VVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRNGFCSKLWH 644
           VV+TFIQTLIDEPSSLAGQLVDSFSDIIS KKPRNGFCSKLWH
Sbjct: 611 VVNTFIQTLIDEPSSLAGQLVDSFSDIISCKKPRNGFCSKLWH 653

BLAST of Carg12181-RA vs. TAIR 10
Match: AT3G24440.1 (Fibronectin type III domain-containing protein )

HSP 1 Score: 514.6 bits (1324), Expect = 1.1e-145
Identity = 297/630 (47.14%), Postives = 391/630 (62.06%), Query Frame = 0

Query: 15  NNRSASRKSYRKIDNPARVPTAPGKSIQSG-ISSTWVCKNSACRAVLSVDDTFCKRCSCC 74
           ++R  ++KS +K ++  +           G + S+W+CKN++CRA +  +D+FCKRCSCC
Sbjct: 12  HSRKTNKKSNKKHESNGKQQQQQDVDGGGGCLRSSWICKNASCRANVPKEDSFCKRCSCC 71

Query: 75  ICHLFDDNKDPSLWLVCSTE-SGQRDSCGLSCHIECALQREKVGVVDLGQLMQLDGSYCC 134
           +CH FD+NKDPSLWLVC  E S   + CGLSCHIECA +  KVGV+ LG LM+LDG +CC
Sbjct: 72  VCHNFDENKDPSLWLVCEPEKSDDVEFCGLSCHIECAFREVKVGVIALGNLMKLDGCFCC 131

Query: 135 ASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLLERTSRFKELHEIIQDAKAKL 194
            SCGKVS IL CWKKQL  A++ARR D LCYRI LGYRLL  TSRF ELHEI++ AK+ L
Sbjct: 132 YSCGKVSQILGCWKKQLVAAKEARRRDGLCYRIDLGYRLLNGTSRFSELHEIVRAAKSML 191

Query: 195 ETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEKADQWLAGVSNTNLNCRDDSFPA 254
           E EVGP++G +A+  RGIV RL V  +VQ+LC+ AI+KA +  A       N   D  PA
Sbjct: 192 EDEVGPLDGPTARTDRGIVSRLPVAANVQELCTSAIKKAGELSA-------NAGRDLVPA 251

Query: 255 ACKLLFEEIKYSSIVITLVEISNSSSNETKGYKLWYSKSREETYTEEPICV-FPRAQRRI 314
           AC+  FE+I    + + L+E+ ++   + KGYKLWY K  E    E+ + V   R +RR+
Sbjct: 252 ACRFHFEDIAPKQVTLRLIELPSAVEYDVKGYKLWYFKKGE--MPEDDLFVDCSRTERRM 311

Query: 315 LISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVEIIHNSHSPAPSNHRKESPVTEES 374
           +IS+L+PCTEY FR+VSY+E G  GHS A CFTKSVEI+       P + +++  +    
Sbjct: 312 VISDLEPCTEYTFRVVSYTEAGIFGHSNAMCFTKSVEIL------KPVDGKEKRTI---D 371

Query: 375 CIRKKGPDDTTIMCS-SSGFQVRDLGKILELARAQGEGCLESLCCADVKNCCRVQMGVKP 434
            +    P D     S SS FQ+  LGK ++LA AQ EG LE+    D +  C        
Sbjct: 372 LVGNAQPSDREEKSSISSRFQIGQLGKYVQLAEAQEEGLLEAFYNVDTEKIC-------- 431

Query: 435 ETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRDEVNGCTLQQAVEA---DEDAAS 494
           E PEEE  P    G DLNVVSVPDLNEE TPP +    E NG  L    EA   D D   
Sbjct: 432 EPPEEELPPRRPHGFDLNVVSVPDLNEEFTPP-DSSGGEDNGVPLNSLAEADGGDHDDNC 491

Query: 495 HDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKRAASTSEDPN-DCDSTLING 554
            D   NG  R   ++D  + + G +G+    D L    RK  + +++  N +CDS+ I  
Sbjct: 492 DDAVSNG--RRKNNNDCLVISDG-SGDDTGFDFLMTRKRKAISDSNDSENHECDSSSI-- 551

Query: 555 SPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLTWFSLRSTEQERRVVSTFI 614
                       D+  E CVK+IRWLE EGHIK  FR++ LTWFS+ ST QE+ VVSTF+
Sbjct: 552 ------------DDTLEKCVKVIRWLEREGHIKTTFRVRFLTWFSMSSTAQEQSVVSTFV 597

Query: 615 QTLIDEPSSLAGQLVDSFSDIISSKKPRNG 637
           QTL D+P SLAGQLVD+F+D++S+K+P NG
Sbjct: 612 QTLEDDPGSLAGQLVDAFTDVVSTKRPNNG 597

BLAST of Carg12181-RA vs. TAIR 10
Match: AT4G30200.1 (vernalization5/VIN3-like )

HSP 1 Score: 332.8 bits (852), Expect = 6.1e-91
Identity = 224/670 (33.43%), Postives = 326/670 (48.66%), Query Frame = 0

Query: 8   KKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQS-------------GISSTWVCKNS 67
           KK  S       + K  RK+DNP+R        + S             G S+T  CKN 
Sbjct: 73  KKRDSDCLPIQRNTKRQRKVDNPSRYVIPATNIVTSNNASGSCSSVNTKGESTTIYCKNL 132

Query: 68  ACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESG-QRDSCGLSCHIECALQRE 127
           ACRAVL  +D+FC+RCSCCIC  +DDNKDPSLWL CS++   + +SCG SCH+ECA   E
Sbjct: 133 ACRAVLRQEDSFCRRCSCCICRKYDDNKDPSLWLTCSSDPPFEGESCGFSCHLECAFNTE 192

Query: 128 KVGVVDLGQLMQLDG-SYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLL 187
           K G   LG+  Q +G  + C SCGK + +LECWKKQL IA++ RRV+VLCYR++L  +LL
Sbjct: 193 KSG---LGKDKQSEGCCFYCVSCGKANSLLECWKKQLTIAKETRRVEVLCYRLFLVQKLL 252

Query: 188 ERTSRFKELHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEK-- 247
           + +++++ L E++ +A   LE +VGP+ G+  KM RGIV RL  G DVQKLCS A+E   
Sbjct: 253 KSSTKYRNLCEVVDEAVKTLEADVGPLTGLPMKMGRGIVNRLHSGPDVQKLCSSALESLE 312

Query: 248 ------ADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGY 307
                  D        ++   +D +   + K+ FE++  +S+ + L      S      Y
Sbjct: 313 TIATTPPDVAALPSPRSSKMQQDTATTGSTKIRFEDVNATSLTVVLASNEIPSPPNIVHY 372

Query: 308 KLWYSKSREETYTEEPICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFT 367
            +W+ K  E+ Y E+  C       R ++S L P +EY F++VSYS   ++G  E    T
Sbjct: 373 SIWHRKVPEKDYPEKSTCTLFIPNTRFVVSGLAPASEYCFKVVSYSGTREMGVDEINVLT 432

Query: 368 KSVEIIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARA 427
           +S E   N  S      R  SP+T  +C        +T+  + S  +       +     
Sbjct: 433 RSAEEGANCSSAV---ERSVSPLT--NC--------STLSSNPSSVEAESNNDYI----- 492

Query: 428 QGEGCLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFE 487
                         K   + +    P   +E     + R  D ++V +    E++     
Sbjct: 493 -----------VPKKPSSKNEDNNSPSV-DESAAKRMKRTTDSDIVQIEKDVEQIV---- 552

Query: 488 CFRDEVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGL 547
              DE     L +     E      +    L  +  S D+ +    P     + D +   
Sbjct: 553 LLDDEEQEAVLDKT----ESETPVVVTTKSLVGNRNSSDASL----PITPFRS-DEIKNR 612

Query: 548 YRKRAASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRL 607
             +   S  ++ N+ D +   G+           +   E+CVKIIR LEC GHI + FR 
Sbjct: 613 QARIEISMKDNCNNGDHSANGGT-----------ESGLEHCVKIIRQLECSGHIDKNFRQ 672

Query: 608 KLLTWFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPR---------- 644
           K LTW+SLR+T QE RVV  FI T ID+P +LA QL+D+F D +S K+            
Sbjct: 673 KFLTWYSLRATSQEIRVVKIFIDTFIDDPMALAEQLIDTFDDRVSIKRSAVGGSGASAVV 685

BLAST of Carg12181-RA vs. TAIR 10
Match: AT4G30200.3 (vernalization5/VIN3-like )

HSP 1 Score: 332.8 bits (852), Expect = 6.1e-91
Identity = 224/670 (33.43%), Postives = 326/670 (48.66%), Query Frame = 0

Query: 8   KKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQS-------------GISSTWVCKNS 67
           KK  S       + K  RK+DNP+R        + S             G S+T  CKN 
Sbjct: 90  KKRDSDCLPIQRNTKRQRKVDNPSRYVIPATNIVTSNNASGSCSSVNTKGESTTIYCKNL 149

Query: 68  ACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESG-QRDSCGLSCHIECALQRE 127
           ACRAVL  +D+FC+RCSCCIC  +DDNKDPSLWL CS++   + +SCG SCH+ECA   E
Sbjct: 150 ACRAVLRQEDSFCRRCSCCICRKYDDNKDPSLWLTCSSDPPFEGESCGFSCHLECAFNTE 209

Query: 128 KVGVVDLGQLMQLDG-SYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLL 187
           K G   LG+  Q +G  + C SCGK + +LECWKKQL IA++ RRV+VLCYR++L  +LL
Sbjct: 210 KSG---LGKDKQSEGCCFYCVSCGKANSLLECWKKQLTIAKETRRVEVLCYRLFLVQKLL 269

Query: 188 ERTSRFKELHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEK-- 247
           + +++++ L E++ +A   LE +VGP+ G+  KM RGIV RL  G DVQKLCS A+E   
Sbjct: 270 KSSTKYRNLCEVVDEAVKTLEADVGPLTGLPMKMGRGIVNRLHSGPDVQKLCSSALESLE 329

Query: 248 ------ADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITLVEISNSSSNETKGY 307
                  D        ++   +D +   + K+ FE++  +S+ + L      S      Y
Sbjct: 330 TIATTPPDVAALPSPRSSKMQQDTATTGSTKIRFEDVNATSLTVVLASNEIPSPPNIVHY 389

Query: 308 KLWYSKSREETYTEEPICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFT 367
            +W+ K  E+ Y E+  C       R ++S L P +EY F++VSYS   ++G  E    T
Sbjct: 390 SIWHRKVPEKDYPEKSTCTLFIPNTRFVVSGLAPASEYCFKVVSYSGTREMGVDEINVLT 449

Query: 368 KSVEIIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARA 427
           +S E   N  S      R  SP+T  +C        +T+  + S  +       +     
Sbjct: 450 RSAEEGANCSSAV---ERSVSPLT--NC--------STLSSNPSSVEAESNNDYI----- 509

Query: 428 QGEGCLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFE 487
                         K   + +    P   +E     + R  D ++V +    E++     
Sbjct: 510 -----------VPKKPSSKNEDNNSPSV-DESAAKRMKRTTDSDIVQIEKDVEQIV---- 569

Query: 488 CFRDEVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGL 547
              DE     L +     E      +    L  +  S D+ +    P     + D +   
Sbjct: 570 LLDDEEQEAVLDKT----ESETPVVVTTKSLVGNRNSSDASL----PITPFRS-DEIKNR 629

Query: 548 YRKRAASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRL 607
             +   S  ++ N+ D +   G+           +   E+CVKIIR LEC GHI + FR 
Sbjct: 630 QARIEISMKDNCNNGDHSANGGT-----------ESGLEHCVKIIRQLECSGHIDKNFRQ 689

Query: 608 KLLTWFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPR---------- 644
           K LTW+SLR+T QE RVV  FI T ID+P +LA QL+D+F D +S K+            
Sbjct: 690 KFLTWYSLRATSQEIRVVKIFIDTFIDDPMALAEQLIDTFDDRVSIKRSAVGGSGASAVV 702

BLAST of Carg12181-RA vs. TAIR 10
Match: AT4G30200.2 (vernalization5/VIN3-like )

HSP 1 Score: 330.5 bits (846), Expect = 3.0e-90
Identity = 226/683 (33.09%), Postives = 329/683 (48.17%), Query Frame = 0

Query: 8   KKASSSLNNRSASRKSYRKIDNPARVPTAPGKSIQS-------------GISSTWVCKNS 67
           KK  S       + K  RK+DNP+R        + S             G S+T  CKN 
Sbjct: 90  KKRDSDCLPIQRNTKRQRKVDNPSRYVIPATNIVTSNNASGSCSSVNTKGESTTIYCKNL 149

Query: 68  ACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESG-QRDSCGLSCHIECALQRE 127
           ACRAVL  +D+FC+RCSCCIC  +DDNKDPSLWL CS++   + +SCG SCH+ECA   E
Sbjct: 150 ACRAVLRQEDSFCRRCSCCICRKYDDNKDPSLWLTCSSDPPFEGESCGFSCHLECAFNTE 209

Query: 128 KVGVVDLGQLMQLDG-SYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYRLL 187
           K G   LG+  Q +G  + C SCGK + +LECWKKQL IA++ RRV+VLCYR++L  +LL
Sbjct: 210 KSG---LGKDKQSEGCCFYCVSCGKANSLLECWKKQLTIAKETRRVEVLCYRLFLVQKLL 269

Query: 188 ERTSRFKELHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIE--- 247
           + +++++ L E++ +A   LE +VGP+ G+  KM RGIV RL  G DVQKLCS A+E   
Sbjct: 270 KSSTKYRNLCEVVDEAVKTLEADVGPLTGLPMKMGRGIVNRLHSGPDVQKLCSSALESLE 329

Query: 248 ------------------KADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITLV 307
                             K  Q  + V +  ++  D +   + K+ FE++  +S+ + L 
Sbjct: 330 TIATTPPDVAALPSPRSSKMQQDCSYVLSNEISA-DTATTGSTKIRFEDVNATSLTVVLA 389

Query: 308 EISNSSSNETKGYKLWYSKSREETYTEEPICVFPRAQRRILISNLQPCTEYMFRIVSYSE 367
                S      Y +W+ K  E+ Y E+  C       R ++S L P +EY F++VSYS 
Sbjct: 390 SNEIPSPPNIVHYSIWHRKVPEKDYPEKSTCTLFIPNTRFVVSGLAPASEYCFKVVSYSG 449

Query: 368 NGDVGHSEAKCFTKSVEIIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQ 427
             ++G  E    T+S E   N  S      R  SP+T  +C        +T+  + S  +
Sbjct: 450 TREMGVDEINVLTRSAEEGANCSSAV---ERSVSPLT--NC--------STLSSNPSSVE 509

Query: 428 VRDLGKILELARAQGEGCLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVS 487
                  +                   K   + +    P   +E     + R  D ++V 
Sbjct: 510 AESNNDYI----------------VPKKPSSKNEDNNSPSV-DESAAKRMKRTTDSDIVQ 569

Query: 488 VPDLNEELTPPFECFRDEVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGP 547
           +    E++        DE     L +     E      +    L  +  S D+ +    P
Sbjct: 570 IEKDVEQIV----LLDDEEQEAVLDKT----ESETPVVVTTKSLVGNRNSSDASL----P 629

Query: 548 NGEVPAVDSLTGLYRKRAASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRW 607
                + D +     +   S  ++ N+ D +   G+           +   E+CVKIIR 
Sbjct: 630 ITPFRS-DEIKNRQARIEISMKDNCNNGDHSANGGT-----------ESGLEHCVKIIRQ 689

Query: 608 LECEGHIKQEFRLKLLTWFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSK 644
           LEC GHI + FR K LTW+SLR+T QE RVV  FI T ID+P +LA QL+D+F D +S K
Sbjct: 690 LECSGHIDKNFRQKFLTWYSLRATSQEIRVVKIFIDTFIDDPMALAEQLIDTFDDRVSIK 714

BLAST of Carg12181-RA vs. TAIR 10
Match: AT5G57380.1 (Fibronectin type III domain-containing protein )

HSP 1 Score: 297.0 bits (759), Expect = 3.7e-80
Identity = 210/601 (34.94%), Postives = 307/601 (51.08%), Query Frame = 0

Query: 51  CKNSACRAVLSVDDTFCKRCSCCICHLFDDNKDPSLWLVCSTESGQRDSCGLSCHIECAL 110
           C+N ACRA L  DDTFC+RCSCCIC  FDDNKDPSLWL C       D+CG SCH+EC L
Sbjct: 130 CENLACRAALGCDDTFCRRCSCCICQKFDDNKDPSLWLTC-------DACGSSCHLECGL 189

Query: 111 QREKVGVVDLGQLMQLDGSYCCASCGKVSGILECWKKQLAIARDARRVDVLCYRIYLGYR 170
           ++++ G+        LDG + CA CGK + +L CW+KQ+ +A++ RRVDVLCYR+ LG +
Sbjct: 190 KQDRYGIGS----DDLDGRFYCAYCGKDNDLLGCWRKQVKVAKETRRVDVLCYRLSLGQK 249

Query: 171 LLERTSRFKELHEIIQDAKAKLETEVGPVNGISAKMARGIVCRLSVGGDVQKLCSFAIEK 230
           LL  T++++ L E++ +A  KLE +VGP++G + KMARGIV RLS G  VQKLCS A+E 
Sbjct: 250 LLRGTTKYRNLLELMDEAVKKLEGDVGPLSGWAMKMARGIVNRLSSGVHVQKLCSQAMEA 309

Query: 231 ADQWLAGVSNTNLNCRDDSFPAACKLLFEEIKYSSIVITL--VEISNSSSNETKGYKLWY 290
            D+ ++   + +++ + D       +  EEI+  S+ + +   E S+S+ N+  G++L+ 
Sbjct: 310 LDKVVS--PSESVSGQGDKM----TVRVEEIQARSVTVRVDSEEPSSSTQNKITGFRLFC 369

Query: 291 SKSREETYTEEPICVFPRAQRRILISNLQPCTEYMFRIVSYSENGDVGHSEAKCFTKSVE 350
            KS++E  + +  CV    +    I  L+P TE+  R+VS++E GD+  SE +  T    
Sbjct: 370 RKSKDEECSSQGNCVVYLPETTSAIQGLEPDTEFCLRVVSFNEEGDLDESELRFTTLK-- 429

Query: 351 IIHNSHSPAPSNHRKESPVTEESCIRKKGPDDTTIMCSSSGFQVRDLGKILELARAQGEG 410
                         ++SP+T  S          + +CS+      D    +  + ++G G
Sbjct: 430 ------DDGDEAGDQQSPLTNSS----------SGLCSNPSLP-EDESNNVNKSCSKGNG 489

Query: 411 CLESLCCADVKNCCRVQMGVKPETPEEEQLPPVSRGLDLNVVSVPDLNEELTPPFECFRD 470
                   D  N      G      EEE+L  V R    N +   DL   L  P  C RD
Sbjct: 490 --------DKDNTEHCSAGEVESELEEERL--VKR--KANKIDGRDL---LVTP--CKRD 549

Query: 471 EVNGCTLQQAVEADEDAASHDIEKNGLARSHGSDDSQIWTCGPNGEVPAVDSLTGLYRKR 530
              G                  ++ G  R                           ++ R
Sbjct: 550 IYKG------------------KQGGNKR---------------------------FKSR 609

Query: 531 AASTSEDPNDCDSTLINGSPLPLSNGSCFLDENFEYCVKIIRWLECEGHIKQEFRLKLLT 590
             S +E P       IN +    +NG    D++  + VK IR LE EGHI + FR + LT
Sbjct: 610 TVSLNEKPE------INNA----ANG--VGDKDLGHIVKTIRCLEEEGHIDKSFRERFLT 620

Query: 591 WFSLRSTEQERRVVSTFIQTLIDEPSSLAGQLVDSFSDIISSKKPRN------GFCSKLW 644
           W+SLR+T +E RVV  F++T +++ SSL  QLVD+FS+ I SK+         G C KLW
Sbjct: 670 WYSLRATHREVRVVKIFVETFMEDLSSLGQQLVDTFSESILSKRSSTNGVVPAGICLKLW 620

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021834.10.0e+00100.00VIN3-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022932185.10.0e+0099.53VIN3-like protein 1 [Cucurbita moschata][more]
KAG6587945.10.0e+0099.53VIN3-like protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023530610.10.0e+0098.29VIN3-like protein 1 [Cucurbita pepo subsp. pepo][more]
XP_023002374.10.0e+0097.67VIN3-like protein 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LHF51.6e-14447.14VIN3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=VIL1 PE=1 SV=1[more]
Q9SUM44.3e-8933.09VIN3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=VIL2 PE=1 SV=1[more]
Q9FIE35.2e-7934.94Protein VERNALIZATION INSENSITIVE 3 OS=Arabidopsis thaliana OX=3702 GN=VIN3 PE=1... [more]
Q5BPT41.2e-5127.63VIN3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=VIL3 PE=2 SV=1[more]
Q94B719.4e-0424.12Protein OBERON 3 OS=Arabidopsis thaliana OX=3702 GN=OBE3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F1I50.0e+0099.53VIN3-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438501 PE=4 SV=1[more]
A0A6J1KQA10.0e+0097.67VIN3-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111496230 PE=4 SV=1[more]
A0A5D3BLD10.0e+0088.49VIN3-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CJJ20.0e+0088.02VIN3-like protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501541 PE=4 SV=1[more]
A0A5A7TZG40.0e+0088.02VIN3-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
Match NameE-valueIdentityDescription
AT3G24440.11.1e-14547.14Fibronectin type III domain-containing protein [more]
AT4G30200.16.1e-9133.43vernalization5/VIN3-like [more]
AT4G30200.36.1e-9133.43vernalization5/VIN3-like [more]
AT4G30200.23.0e-9033.09vernalization5/VIN3-like [more]
AT5G57380.13.7e-8034.94Fibronectin type III domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032881Oberon, PHD finger domainPFAMPF07227PHD_Oberoncoord: 51..169
e-value: 5.4E-32
score: 110.4
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 251..348
e-value: 1.5E-7
score: 33.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 353..374
NoneNo IPR availablePANTHERPTHR46286:SF1VIN3-LIKE PROTEIN 1coord: 2..643
IPR044514Vernalization insensitive 3-likePANTHERPTHR46286VIN3-LIKE PROTEIN 2-RELATEDcoord: 2..643
IPR003961Fibronectin type IIIPROSITEPS50853FN3coord: 250..349
score: 11.716306
IPR003961Fibronectin type IIICDDcd00063FN3coord: 255..344
e-value: 2.88615E-4
score: 38.2463
IPR036116Fibronectin type III superfamilySUPERFAMILY49265Fibronectin type IIIcoord: 259..339

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12181Carg12181gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12181-RA:1Carg12181-RA:1exon
Carg12181-RA:2Carg12181-RA:2exon
Carg12181-RA:3Carg12181-RA:3exon
Carg12181-RA:4Carg12181-RA:4exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12181-RA:five_prime_utrCarg12181-RA:five_prime_utrfive_prime_UTR
Carg12181-RA:five_prime_utrCarg12181-RA:five_prime_utr_2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12181-RA:cdsCarg12181-RA:cdsCDS
Carg12181-RA:cdsCarg12181-RA:cds_2CDS
Carg12181-RA:cdsCarg12181-RA:cds_3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12181-RA:three_prime_utrCarg12181-RA:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12181-RACarg12181-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0040029 regulation of gene expression, epigenetic
biological_process GO:0010048 vernalization response
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding