Carg12162-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12162-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	2-alkenal reductase (NADP(+)-dependent)-like
Location	Carg_Chr11: 2511468 .. 2512789 (-)
Sequence length	567
RNA-Seq Expression	Carg12162-RA
Synteny	Carg12162-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGAACAAGTGATTAACACCTTTGTTACAATAAAACACCCTATCGATGGCTTCCCACATGCCTCAGATTTTGAGCTTAAATCCCAACCTCTTCATCTCTCTGTAAACCCCGGCCATATTGTCCTAAAGAATCTGTGTGTTTCCATTGACCCATATCAGATGAACCGTATGAAGCTCCACAGCTCTTCACATGCAGTTTCATCTGCCACTCGTTTTATCACTCCTGGACAGGTAAGTATTCAAAGTTTTTACCTTTAGATAGTTGGTATATGTCTTGAGATTATGTTTGTGTTTTGGTGGCTTTTGGGTTTGTTTGGAACAGGCGATTAGTGCTTATGGTGTAGCAGAAGTTGTGGGTTCAAGCGACTCTGAATTTGAAAAGCGGGACTTGGTTAAAGGGTGTTGGATGATGGAAGTCCCACGTCGGCTAATTTAGGGAATGATCATGGGTTTATAAGTAAGGATTACTAACTTCATTGGTACGAGGCCTTTTGGGGAAGCCCAAAACAAATCCATGAGAGCGGAAAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACAAGGGATTCATACATTGGGCGGAATACAGCGTTGTTAAGGCTGAAGAATGCACTCTAACAAAGTTGGATACTTTGGGATTTCCCTTAACATACTATCTAGGAATTTTAGGTGAGTTGTAGGGCTGGTTTCGAATCAATTTTTGTAAGATCTAATATTCTTGGTGTGGAAACCTCTCTCTAGTGGGCGTGTTTTAAAACCTTAAGGGAAAGCCTAACGAGAACAATATTTGCTAGTGGTGGGCTTGGGCCATTATAAATGGTATCAGAACCAGACACCGGGCGATGTGATAGTGAGGACAATGGCCCCCAAGGGAGTGAGATGTGAGATCCCAGGTCGGTTGGAAAGTGGAATGAAACATTTCTTATAAAGTGTGGAAACTTGTCCCTAGAGAACGTATTTCAAAACCTTGAAGTGAAATCTACAATATCTGCTAGCGATGGGCTTAAATCATTACACTTTTAGACCGTTTTTAAGTAAAAAAAAGAAAAGCTTTAAAACACTTTGGAAGAATATAATATACTATGAAGAAACACTTATTTTCCTTTTGTGGTTGATCAGGGATGAGTGGCCACACTGCCTACACTGGTCTGTTTGAAGTAGGGAAGGTTAAGGAAGGGGACAAAGTGTTTGTGTCTGCAGCATCTGGCTCTGTTGGGAATTTAGTTGGACAGTTTGCTAAGCTGCATGGTTGCTATGTTGTTGGCTGTGCTGGTTCTGATCAAAAGGTCCAACTTTTCACAAACAATCAATATTAA

mRNA sequence

ATGGAGGAACAAGTGATTAACACCTTTGTTACAATAAAACACCCTATCGATGGCTTCCCACATGCCTCAGATTTTGAGCTTAAATCCCAACCTCTTCATCTCTCTGTAAACCCCGGCCATATTGTCCTAAAGAATCTGTGTGTTTCCATTGACCCATATCAGATGAACCGTATGAAGCTCCACAGCTCTTCACATGCAGTTTCATCTGCCACTCGTTTTATCACTCCTGGACAGGCGATTAGTGCTTATGGTGTAGCAGAAGTTGTGGGTTCAAGCGACTCTGAATTTGAAAAGCGGGACTTGAACCAGACACCGGGCGATGTGATAGTGAGGACAATGGCCCCCAAGGGAGTGAGATGTGAGATCCCAGGGATGAGTGGCCACACTGCCTACACTGGTCTGTTTGAAGTAGGGAAGGTTAAGGAAGGGGACAAAGTGTTTGTGTCTGCAGCATCTGGCTCTGTTGGGAATTTAGTTGGACAGTTTGCTAAGCTGCATGGTTGCTATGTTGTTGGCTGTGCTGGTTCTGATCAAAAGGTCCAACTTTTCACAAACAATCAATATTAA

Coding sequence (CDS)

ATGGAGGAACAAGTGATTAACACCTTTGTTACAATAAAACACCCTATCGATGGCTTCCCACATGCCTCAGATTTTGAGCTTAAATCCCAACCTCTTCATCTCTCTGTAAACCCCGGCCATATTGTCCTAAAGAATCTGTGTGTTTCCATTGACCCATATCAGATGAACCGTATGAAGCTCCACAGCTCTTCACATGCAGTTTCATCTGCCACTCGTTTTATCACTCCTGGACAGGCGATTAGTGCTTATGGTGTAGCAGAAGTTGTGGGTTCAAGCGACTCTGAATTTGAAAAGCGGGACTTGAACCAGACACCGGGCGATGTGATAGTGAGGACAATGGCCCCCAAGGGAGTGAGATGTGAGATCCCAGGGATGAGTGGCCACACTGCCTACACTGGTCTGTTTGAAGTAGGGAAGGTTAAGGAAGGGGACAAAGTGTTTGTGTCTGCAGCATCTGGCTCTGTTGGGAATTTAGTTGGACAGTTTGCTAAGCTGCATGGTTGCTATGTTGTTGGCTGTGCTGGTTCTGATCAAAAGGTCCAACTTTTCACAAACAATCAATATTAA

Protein sequence

MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKLHSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQTPGDVIVRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKVQLFTNNQY

Homology

[Close]

BLAST of Carg12162-RA vs. NCBI nr
Match: KAG7021816.1 (2-alkenal reductase (NADP(+)-dependent), partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384.8 bits (987), Expect = 4.3e-103
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKL 60
           MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKL
Sbjct: 1   MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKL 60

Query: 61  HSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQTPGDVIVRTMAPKGVRC 120
           HSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQTPGDVIVRTMAPKGVRC
Sbjct: 61  HSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQTPGDVIVRTMAPKGVRC 120

Query: 121 EIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKV 180
           EIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKV
Sbjct: 121 EIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKV 180

Query: 181 QLFTNNQY 189
           QLFTNNQY
Sbjct: 181 QLFTNNQY 188

[Close]

BLAST of Carg12162-RA vs. NCBI nr
Match: KAG6587928.1 (2-alkenal reductase (NADP(+)-dependent), partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 297.7 bits (761), Expect = 6.8e-77
Identity = 154/188 (81.91%), Postives = 154/188 (81.91%), Query Frame = 0


Query: 1   MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKL 60
           MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKL
Sbjct: 1   MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKL 60

Query: 61  HSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQTPGDVIVRTMAPKGVRC 120
           HSSSHAVSSATRFITPGQAISAYGVAEVV                               
Sbjct: 61  HSSSHAVSSATRFITPGQAISAYGVAEVV------------------------------- 120

Query: 121 EIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKV 180
              GMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKV
Sbjct: 121 ---GMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKV 154

Query: 181 QLFTNNQY 189
           QLFTNNQY
Sbjct: 181 QLFTNNQY 154

[Close]

BLAST of Carg12162-RA vs. NCBI nr
Match: XP_023530383.1 (2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290.8 bits (743), Expect = 8.4e-75
Identity = 154/197 (78.17%), Postives = 159/197 (80.71%), Query Frame = 0


Query: 1   MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHIVLKNLCVSIDPYQMNRMKL 60
           MEEQVINTFVTIKH IDGFPHASDFELKSQPLHLSV PGHIVLKNLCVSIDPYQ+NRMKL
Sbjct: 1   MEEQVINTFVTIKHSIDGFPHASDFELKSQPLHLSVKPGHIVLKNLCVSIDPYQINRMKL 60

Query: 61  HSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL----------NQTPGDVIV 120
           HSSSH+VSSATRFITPGQAISAYGVAEVV SSDS FEK DL          +    +   
Sbjct: 61  HSSSHSVSSATRFITPGQAISAYGVAEVVASSDSGFEKGDLVKGFIHWAEYSVVKAEECT 120

Query: 121 RTMA-----PKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKL 180
            T       P      I GMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQ+AKL
Sbjct: 121 LTKLDTLGFPLTYYLGILGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQYAKL 180

Query: 181 HGCYVVGCAGSDQKVQL 183
           HGCYVVGCAGSDQKV L
Sbjct: 181 HGCYVVGCAGSDQKVAL 197

[Close]

BLAST of Carg12162-RA vs. NCBI nr
Match: XP_022145088.1 (2-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 2.6e-52
Identity = 123/199 (61.81%), Postives = 139/199 (69.85%), Query Frame = 0


Query: 1   MEEQV--INTFVTIKHPIDGFPHASDFELKSQPLHLSVNP--GHIVLKNLCVSIDPYQMN 60
           M EQV   NTF+ IKH I+GFP  + F+L SQPL+L V P  G I+LKNLCVSIDPYQ+N
Sbjct: 1   MAEQVETNNTFIAIKHLINGFPLQTHFQLNSQPLNLCVKPASGDILLKNLCVSIDPYQIN 60

Query: 61  RMKLHSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL-----------NQTP 120
           RM +HSSSH VSSAT  I PGQAISAYGVAEVV S D  FEK DL               
Sbjct: 61  RMNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKA 120

Query: 121 GDVIVRTMAPKG----VRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQ 180
           G+  +R +   G        + G+SG TAY GLFEV K+KEG+KVFVSAASGSVGNLVGQ
Sbjct: 121 GESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQ 180

[Close]

BLAST of Carg12162-RA vs. NCBI nr
Match: XP_038878370.1 (NADPH-dependent oxidoreductase 2-alkenal reductase-like [Benincasa hispida])

HSP 1 Score: 206.8 bits (525), Expect = 1.6e-49
Identity = 117/193 (60.62%), Postives = 133/193 (68.91%), Query Frame = 0


Query: 7   NTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLHSSS 66
           N+F+TIKH I+GFP  SDFELKS PL LS  PG   IVLKNL VS+DPYQ+NRMK HS S
Sbjct: 8   NSFITIKHHINGFPVESDFELKSHPLRLSAQPGSADIVLKNLSVSVDPYQINRMKTHSPS 67

Query: 67  HAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRD-----LNQTPGDVI---------V 126
           H VSS T +I PGQ I  +GVAEV+ S DSEF+K D     ++     V+         +
Sbjct: 68  HTVSSVTPYIIPGQVIDVHGVAEVMDSDDSEFQKGDFVKGIIHWAQYSVVKAGESALMKL 127

Query: 127 RTMA-PKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCY 183
            TM  P      I G SG +AY GLF+VG VKEGD VFVSAASGSVGNLVGQFAKL GCY
Sbjct: 128 DTMEFPLTYYLGILGTSGLSAYAGLFDVGNVKEGDNVFVSAASGSVGNLVGQFAKLQGCY 187

[Close]

BLAST of Carg12162-RA vs. ExPASy Swiss-Prot
Match: Q9SLN8 (2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-31
Identity = 86/198 (43.43%), Postives = 112/198 (56.57%), Query Frame = 0


Query: 1   MEEQVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRM 60
           M E+V N  V +K+ + G+P  SD E+K+  + L V  G   +V+KNL +S DPY  +RM
Sbjct: 1   MAEEVSNKQVILKNYVTGYPKESDMEIKNVTIKLKVPEGSNDVVVKNLYLSCDPYMRSRM 60

Query: 61  KLHSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQ-----------TPGD 120
           +    S+  S A     PG  I+ YGVA+V+ S D +F+K DL             TP  
Sbjct: 61  RKIEGSYVESFA-----PGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQ 120

Query: 121 VIVRTM---APKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAK 180
            + +      P      I GM G TAY G  EV   K+G+ VFVSAASG+VG LVGQFAK
Sbjct: 121 TLFKIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAK 180

Query: 181 LHGCYVVGCAGSDQKVQL 183
           + GCYVVG AGS +KV L
Sbjct: 181 MLGCYVVGSAGSKEKVDL 193

[Close]

BLAST of Carg12162-RA vs. ExPASy Swiss-Prot
Match: Q39172 (NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=3702 GN=AER PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-27
Identity = 76/193 (39.38%), Postives = 105/193 (54.40%), Query Frame = 0


Query: 7   NTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLHSSS 66
           N  V +K  + GFP  SDF+  +  + L V  G   +++KNL +S DPY   R+++    
Sbjct: 5   NKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYM--RIRMGKPD 64

Query: 67  HAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQ-----------TPGD----VI 126
            + ++  +  TPGQ I  YGV+ ++ S   +++K DL             TP       I
Sbjct: 65  PSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKI 124

Query: 127 VRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCY 183
             T  P      + GM G TAY G +EV   KEG+ V+VSAASG+VG LVGQ AK+ GCY
Sbjct: 125 QHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCY 184

[Close]

BLAST of Carg12162-RA vs. ExPASy Swiss-Prot
Match: Q39173 (NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 GN=P2 PE=2 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 3.0e-27
Identity = 79/193 (40.93%), Postives = 103/193 (53.37%), Query Frame = 0


Query: 7   NTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLHSSS 66
           N  V  K  + GFP  SDF   +  + L V  G   +++KNL +S DPY  +RM     S
Sbjct: 4   NKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRMGKPDPS 63

Query: 67  HAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQ-----------TP----GDVI 126
            A++ A     PG+ I  YGV+ V+ S   +++K DL             TP       I
Sbjct: 64  SALAQA---YAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMAHMHFKI 123

Query: 127 VRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCY 183
             T  P      + GM G TAY G +EV   K+G+ V+VSAASG+VG LVGQFAK+ GCY
Sbjct: 124 QHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCY 183

[Close]

BLAST of Carg12162-RA vs. ExPASy Swiss-Prot
Match: Q6WAU0 ((+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.1e-24
Identity = 75/199 (37.69%), Postives = 107/199 (53.77%), Query Frame = 0


Query: 5   VINTFVTIKHPIDGFPHASDFELKSQPLHLSVNP---GHIVLKNLCVSIDPYQMNRMKLH 64
           V+N  + + + I+G    SD  L++  + + +     G I++KNL +S++PY + RM   
Sbjct: 2   VMNKQIVLNNYINGSLKQSDLALRTSTICMEIPDGCNGAILVKNLYLSVNPYLILRMGKL 61

Query: 65  SSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRD-------------LNQTPGDV 124
                 S     I PG  I +YGV++V+ S+   +EK +             L Q P ++
Sbjct: 62  DIPQFDS-----ILPGSTIVSYGVSKVLDSTHPSYEKGELIWGSQAGWEEYTLIQNPYNL 121

Query: 125 --IVRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLH 184
             I     P      I GM G TAY G FE+   K+G+ VFV+AA+GSVG LVGQFAK+ 
Sbjct: 122 FKIQDKDVPLSYYVGILGMPGMTAYAGFFEICSPKKGETVFVTAAAGSVGQLVGQFAKMF 181

Query: 185 GCYVVGCAGSDQKVQLFTN 186
           GCYVVG AGS +KV L  N
Sbjct: 182 GCYVVGSAGSKEKVDLLKN 195

[Close]

BLAST of Carg12162-RA vs. ExPASy Swiss-Prot
Match: N4WR35 (NADP-dependent oxidoreductase RED1 OS=Cochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) OX=665024 GN=RED1 PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.7e-23
Identity = 67/198 (33.84%), Postives = 106/198 (53.54%), Query Frame = 0


Query: 7   NTFVTIKHPIDGFPHAS-DFELKSQPLHLS--VNPGHIVLKNLCVSIDPYQMNRMKLHSS 66
           N  +  K+P  G+P    DF ++ +P+ ++  +  G ++LKN  VS+DPYQ  RM+    
Sbjct: 4   NKSLIFKNPPIGWPKPDVDFGVECRPIDINGDIPLGGVILKNCYVSLDPYQRGRMR---- 63

Query: 67  SHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQTPGDVIVRTMAPKGV----- 126
           +  V S +   T G  +  + ++ V+ S+ ++ +  D     G +   ++   GV     
Sbjct: 64  APTVDSYSPPFTIGDPMEGHVISRVIRSASTKLQPGDFVSGVGPIQEFSVLSAGVVDGFT 123

Query: 127 RCEIP------------GMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLH 185
           R E P            GM G TAY+  +E+GK K+G+ +F+SAASG+VG LVGQ AK  
Sbjct: 124 RIENPYNLNLEIFLGPLGMPGLTAYSSFYEIGKPKKGETIFISAASGAVGQLVGQLAKRE 183

[Close]

BLAST of Carg12162-RA vs. ExPASy TrEMBL
Match: A0A6J1CV06 (2-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=LOC111014596 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.3e-52
Identity = 123/199 (61.81%), Postives = 139/199 (69.85%), Query Frame = 0


Query: 1   MEEQV--INTFVTIKHPIDGFPHASDFELKSQPLHLSVNP--GHIVLKNLCVSIDPYQMN 60
           M EQV   NTF+ IKH I+GFP  + F+L SQPL+L V P  G I+LKNLCVSIDPYQ+N
Sbjct: 1   MAEQVETNNTFIAIKHLINGFPLQTHFQLNSQPLNLCVKPASGDILLKNLCVSIDPYQIN 60

Query: 61  RMKLHSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL-----------NQTP 120
           RM +HSSSH VSSAT  I PGQAISAYGVAEVV S D  FEK DL               
Sbjct: 61  RMNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKA 120

Query: 121 GDVIVRTMAPKG----VRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQ 180
           G+  +R +   G        + G+SG TAY GLFEV K+KEG+KVFVSAASGSVGNLVGQ
Sbjct: 121 GESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQ 180

[Close]

BLAST of Carg12162-RA vs. ExPASy TrEMBL
Match: A0A5N6QL65 (PKS_ER domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_003368 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.3e-45
Identity = 103/195 (52.82%), Postives = 133/195 (68.21%), Query Frame = 0


Query: 4   QVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLH 63
           +V N+++TIK  I+G P  SDFELK+  L LSV PG  H+++KNL +S+DPYQ+NRMK H
Sbjct: 2   EVSNSYITIKTHINGAPKESDFELKTATLALSVEPGSNHVIVKNLYISVDPYQINRMKTH 61

Query: 64  SSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL----------NQTPGDVIVR 123
           SSS      +R ITPG+ + AYGVA+VV S + +FEK +L          +    + ++ 
Sbjct: 62  SSSQKALIDSRRITPGEVVDAYGVAKVVASGNPKFEKGELVVGRIGWEEHSVIKEENLIN 121

Query: 124 TMAPKG----VRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHG 183
            + P G        + G+SG TAY G FEV K K+G+KVFVSAASGSVGNLVGQ+AKL G
Sbjct: 122 KLDPMGFPLTYHVGVLGISGWTAYAGFFEVCKPKKGEKVFVSAASGSVGNLVGQYAKLFG 181

[Close]

BLAST of Carg12162-RA vs. ExPASy TrEMBL
Match: A0A6P8CGZ7 (2-alkenal reductase (NADP(+)-dependent)-like OS=Punica granatum OX=22663 GN=LOC116196683 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.3e-45
Identity = 105/195 (53.85%), Postives = 133/195 (68.21%), Query Frame = 0


Query: 4   QVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNP--GHIVLKNLCVSIDPYQMNRMKLH 63
           +V N +VTIK  ++G P  SDFE+K+  L LS++   G I++KNL VSIDPYQ+NRMK +
Sbjct: 2   EVTNKYVTIKRHVEGTPKESDFEIKTVTLCLSIDSGCGDIIVKNLMVSIDPYQINRMKSY 61

Query: 64  SSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL----------NQTPGDVIVR 123
           SSSH   S  + I PG+ I AYGVA V+ S + EFEK DL          ++ P   ++R
Sbjct: 62  SSSHETISYAKGIVPGEEIEAYGVARVLVSENPEFEKGDLVIGVITWGEYSKIPSGRMIR 121

Query: 124 TMAPKGV----RCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHG 183
            + P G+         G SG TAY GLFE+ K K+GDKVFVSAASGSVGNLVGQ+AKL G
Sbjct: 122 KLEPMGLPLSYHVGFLGFSGLTAYAGLFEICKPKKGDKVFVSAASGSVGNLVGQYAKLFG 181

[Close]

BLAST of Carg12162-RA vs. ExPASy TrEMBL
Match: A0A2P6RIX3 (Putative oxidoreductase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0088551 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.7e-45
Identity = 106/196 (54.08%), Postives = 131/196 (66.84%), Query Frame = 0


Query: 4   QVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLH 63
           +V N ++TIK  +DG P  SDFELK+  L LS++PG   I++KNL VSIDPYQ+NRMK  
Sbjct: 2   EVTNRYITIKTQVDGDPKVSDFELKTSNLPLSLDPGSNDIIVKNLYVSIDPYQINRMKSQ 61

Query: 64  SSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL-----------NQTPGDVIV 123
           SSS   ++    ITPG+ I AYGVA+VV S + EFEK DL               G+ ++
Sbjct: 62  SSSQKATATAGVITPGEGIDAYGVAKVVASGNPEFEKDDLIVGYITWGEYSFLKGGNCVL 121

Query: 124 RTMAPKGV----RCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLH 183
           R   P G+    +  I G+SG TAY G FEV K K+G+KVFVSAA GSVGNLVGQ+AKL 
Sbjct: 122 RKFTPMGLPLSYQVGILGLSGLTAYAGFFEVCKPKKGEKVFVSAACGSVGNLVGQYAKLF 181

[Close]

BLAST of Carg12162-RA vs. ExPASy TrEMBL
Match: W9QT11 (NADP-dependent alkenal double bond reductase P1 OS=Morus notabilis OX=981085 GN=L484_022411 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.7e-45
Identity = 105/195 (53.85%), Postives = 129/195 (66.15%), Query Frame = 0


Query: 4   QVINTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLH 63
           +V N ++T+K  ++G P  SDFE+KS  +  SV PG   I+LKNL VSIDPYQ+NR+K H
Sbjct: 2   EVTNRYITVKAHVEGEPTESDFEIKSSTISTSVQPGSNDIILKNLYVSIDPYQINRIKSH 61

Query: 64  SSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL----------NQTPGDVIVR 123
           S+SH  SS    ITPG+ I AYGVA VV S + EFEK DL              G+ ++R
Sbjct: 62  SASHTTSSFAVVITPGETIDAYGVARVVASGNPEFEKGDLVVGMITWGEYCVLKGEYMLR 121

Query: 124 TMAPKG----VRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHG 183
            + P G        I   SG TAY GLFEVGK K+G+KVFVSAASGS+G+LVGQ+AK  G
Sbjct: 122 KLDPMGFPLSYHVGIFAFSGLTAYAGLFEVGKPKKGEKVFVSAASGSIGHLVGQYAKQFG 181

[Close]

BLAST of Carg12162-RA vs. TAIR 10
Match: AT3G59845.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-29
Identity = 83/203 (40.89%), Postives = 108/203 (53.20%), Query Frame = 0


Query: 1   MEEQVINTFVTIKHPIDGFPHASDFEL--KSQPLHLSVNPGH--IVLKNLCVSIDPYQMN 60
           ME  V N  V +++ + G P  SD  +   S P+ L V PG   ++++NL +S DPY M 
Sbjct: 1   METTVKNKQVILRNYVKGIPKESDLMIITASDPVELKVKPGSAAVMVRNLYLSCDPY-MG 60

Query: 61  RMKLHSSSHAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDL-----------NQTP 120
            +    +   ++    FI PG+ I+  GV++V+ S D  F K D+              P
Sbjct: 61  ILMREPTPSTLALLDAFI-PGKPIAGIGVSQVIDSDDPSFTKGDMIWGIVNWEEFSTINP 120

Query: 121 GDVI---VRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQF 180
             +    V    P      I GM G TAY G FE+   K+GD VFVSAASG+VG LVGQF
Sbjct: 121 AAIFKIDVNINVPLSYYTGILGMIGLTAYAGFFEICSPKKGDTVFVSAASGAVGQLVGQF 180

Query: 181 AKLHGCYVVGCAGSDQKVQLFTN 186
           AKL GCYVVG AGS QKV L  N
Sbjct: 181 AKLMGCYVVGSAGSKQKVDLLLN 201

[Close]

BLAST of Carg12162-RA vs. TAIR 10
Match: AT5G16960.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 126.3 bits (316), Expect = 2.5e-29
Identity = 84/198 (42.42%), Postives = 109/198 (55.05%), Query Frame = 0


Query: 6   INTFVTIKHPIDGFPHASDFELKSQPLHLSVNPGHI--VLKNLCVSIDPYQMNRMKLHSS 65
           IN  V +   + GF   SD  + S  + L V  G +  ++KNL +S DPY  NRM+    
Sbjct: 5   INKQVVLLDYVTGFAKESDLVITSTTIDLRVLKGSMTALVKNLYLSCDPYMRNRMR---K 64

Query: 66  SHAVSSAT-RFITPGQAISAYGVAEVVGSSDSEFEKRDLNQ-----------TP----GD 125
              +S AT +  TPG+ IS +GV++V+ S  S++E+ DL             TP      
Sbjct: 65  PDPLSPATAQSFTPGKPISGFGVSKVIDSGHSDYEEGDLIWGAVGWEEYSVITPIPNLHF 124

Query: 126 VIVRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHG 185
            I  T  P      + GM G TAY G +E+   K+GD VFVSAASG+VG LVGQFAKL G
Sbjct: 125 KIHHTNFPLSYYTGLLGMPGMTAYVGFYEICTPKKGDTVFVSAASGAVGQLVGQFAKLMG 184

[Close]

BLAST of Carg12162-RA vs. TAIR 10
Match: AT5G16970.1 (alkenal reductase )

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-28
Identity = 76/193 (39.38%), Postives = 105/193 (54.40%), Query Frame = 0


Query: 7   NTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLHSSS 66
           N  V +K  + GFP  SDF+  +  + L V  G   +++KNL +S DPY   R+++    
Sbjct: 5   NKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYM--RIRMGKPD 64

Query: 67  HAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQ-----------TPGD----VI 126
            + ++  +  TPGQ I  YGV+ ++ S   +++K DL             TP       I
Sbjct: 65  PSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKI 124

Query: 127 VRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCY 183
             T  P      + GM G TAY G +EV   KEG+ V+VSAASG+VG LVGQ AK+ GCY
Sbjct: 125 QHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCY 184

[Close]

BLAST of Carg12162-RA vs. TAIR 10
Match: AT5G17000.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-28
Identity = 75/193 (38.86%), Postives = 106/193 (54.92%), Query Frame = 0


Query: 7   NTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLHSSS 66
           N  V +K+ + GFP  SDF+ K+  +   +  G   +++KNL +S DPY   R+++    
Sbjct: 5   NKQVVLKNYVSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYLSCDPYM--RIRMGKPD 64

Query: 67  HAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQ-----------TPGD----VI 126
            + ++  +   PG+ I  YGV+ V+ S   +++K DL             TP       I
Sbjct: 65  PSTAALAQAYAPGKPIFGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMTHMHFKI 124

Query: 127 VRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCY 183
             T  P      + GM G TAY G +EV   KEG+ V+VSAASG+VG LVGQFAK+ GCY
Sbjct: 125 QHTDIPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQFAKMMGCY 184

[Close]

BLAST of Carg12162-RA vs. TAIR 10
Match: AT5G16990.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-28
Identity = 79/193 (40.93%), Postives = 103/193 (53.37%), Query Frame = 0


Query: 7   NTFVTIKHPIDGFPHASDFELKSQPLHLSVNPG--HIVLKNLCVSIDPYQMNRMKLHSSS 66
           N  V  K  + GFP  SDF   +  + L V  G   +++KNL +S DPY  +RM     S
Sbjct: 4   NKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRMGKPDPS 63

Query: 67  HAVSSATRFITPGQAISAYGVAEVVGSSDSEFEKRDLNQ-----------TP----GDVI 126
            A++ A     PG+ I  YGV+ V+ S   +++K DL             TP       I
Sbjct: 64  SALAQA---YAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMAHMHFKI 123

Query: 127 VRTMAPKGVRCEIPGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCY 183
             T  P      + GM G TAY G +EV   K+G+ V+VSAASG+VG LVGQFAK+ GCY
Sbjct: 124 QHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCY 183

The following BLAST results are available for this feature:

BLAST of Carg12162-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021816.1	4.3e-103	100.00	2-alkenal reductase (NADP(+)-dependent), partial [Cucurbita argyrosperma subsp. ...	[more]
KAG6587928.1	6.8e-77	81.91	2-alkenal reductase (NADP(+)-dependent), partial [Cucurbita argyrosperma subsp. ...	[more]
XP_023530383.1	8.4e-75	78.17	2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita pepo subsp. pepo]	[more]
XP_022145088.1	2.6e-52	61.81	2-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia]	[more]
XP_038878370.1	1.6e-49	60.62	NADPH-dependent oxidoreductase 2-alkenal reductase-like [Benincasa hispida]	[more]

BLAST of Carg12162-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SLN8	1.2e-31	43.43	2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1...	[more]
Q39172	1.8e-27	39.38	NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=37...	[more]
Q39173	3.0e-27	40.93	NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6WAU0	3.1e-24	37.69	(+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1	[more]
N4WR35	7.7e-23	33.84	NADP-dependent oxidoreductase RED1 OS=Cochliobolus heterostrophus (strain C4 / A...	[more]

BLAST of Carg12162-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CV06	1.3e-52	61.81	2-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A5N6QL65	3.3e-45	52.82	PKS_ER domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_003368 ...	[more]
A0A6P8CGZ7	3.3e-45	53.85	2-alkenal reductase (NADP(+)-dependent)-like OS=Punica granatum OX=22663 GN=LOC1...	[more]
A0A2P6RIX3	5.7e-45	54.08	Putative oxidoreductase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0088551 PE=4...	[more]
W9QT11	5.7e-45	53.85	NADP-dependent alkenal double bond reductase P1 OS=Morus notabilis OX=981085 GN=...	[more]

BLAST of Carg12162-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G59845.1	1.9e-29	40.89	Zinc-binding dehydrogenase family protein	[more]
AT5G16960.1	2.5e-29	42.42	Zinc-binding dehydrogenase family protein	[more]
AT5G16970.1	1.3e-28	39.38	alkenal reductase	[more]
AT5G17000.1	2.2e-28	38.86	Zinc-binding dehydrogenase family protein	[more]
AT5G16990.1	2.2e-28	40.93	Zinc-binding dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.180.10		coord: 2..112 e-value: 2.3E-19 score: 71.5
None	No IPR available	GENE3D	3.40.50.720		coord: 122..188 e-value: 1.7E-24 score: 88.3
None	No IPR available	PANTHER	PTHR43205:SF12	OS06G0602900 PROTEIN	coord: 4..185
IPR041694	Oxidoreductase, N-terminal domain	PFAM	PF16884	ADH_N_2	coord: 11..98 e-value: 1.3E-10 score: 41.2
IPR045010	Medium-chain dehydrogenase/reductase	PANTHER	PTHR43205	PROSTAGLANDIN REDUCTASE	coord: 4..185
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 124..185
IPR011032	GroES-like superfamily	SUPERFAMILY	50129	GroES-like	coord: 5..159

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12162	Carg12162	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12162-RA:4	Carg12162-RA:4	exon
Carg12162-RA:3	Carg12162-RA:3	exon
Carg12162-RA:2	Carg12162-RA:2	exon
Carg12162-RA:1	Carg12162-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12162-RA:cds	Carg12162-RA:cds_4	CDS
Carg12162-RA:cds	Carg12162-RA:cds_3	CDS
Carg12162-RA:cds	Carg12162-RA:cds_2	CDS
Carg12162-RA:cds	Carg12162-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12162-RA	Carg12162-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor