Carg11141-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg11141-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	pectinesterase inhibitor-like
Location	Carg_Chr14: 4895037 .. 4895585 (+)
Sequence length	549
RNA-Seq Expression	Carg11141-RA
Synteny	Carg11141-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTCATCTCAGCCATTGTGGTAGTTCTCTGCCTGTGCATGGCGGCAAAGCGCCACGACGCCACCCGCCCCGTCGAGGAAGGGATGGTCCTAATCGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCGGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCCTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGTCCAAGCCGATGGTGCAGGAGGCTCTCCTGGACTGCCTCGACGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATCGCGGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATCACGGATGTGCGCACGTGTGGCACCGAGTTGGGCAGGGAGCGCCCACCGCTGCTGACGGAGAGGAATGAAGTGTTTATTAAGCTGTGTGAAATTGCGCTGACCATTTCCAAGATTGCCCAGGGTGAAGGGAACTAA

mRNA sequence

ATGAAATTCATCTCAGCCATTGTGGTAGTTCTCTGCCTGTGCATGGCGGCAAAGCGCCACGACGCCACCCGCCCCGTCGAGGAAGGGATGGTCCTAATCGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCGGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCCTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGTCCAAGCCGATGGTGCAGGAGGCTCTCCTGGACTGCCTCGACGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATCGCGGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATCACGGATGTGCGCACGTGTGGCACCGAGTTGGGCAGGGAGCGCCCACCGCTGCTGACGGAGAGGAATGAAGTGTTTATTAAGCTGTGTGAAATTGCGCTGACCATTTCCAAGATTGCCCAGGGTGAAGGGAACTAA

Coding sequence (CDS)

ATGAAATTCATCTCAGCCATTGTGGTAGTTCTCTGCCTGTGCATGGCGGCAAAGCGCCACGACGCCACCCGCCCCGTCGAGGAAGGGATGGTCCTAATCGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCGGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCCTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGTCCAAGCCGATGGTGCAGGAGGCTCTCCTGGACTGCCTCGACGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATCGCGGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATCACGGATGTGCGCACGTGTGGCACCGAGTTGGGCAGGGAGCGCCCACCGCTGCTGACGGAGAGGAATGAAGTGTTTATTAAGCTGTGTGAAATTGCGCTGACCATTTCCAAGATTGCCCAGGGTGAAGGGAACTAA

Protein sequence

MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGEGN

Homology

[Close]

BLAST of Carg11141-RA vs. NCBI nr
Match: KAG6581280.1 (Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018002.1 Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357.5 bits (916), Expect = 7.1e-95
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE 180
           ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE
Sbjct: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE 180

Query: 181 GN 183
           GN
Sbjct: 181 GN 182

[Close]

BLAST of Carg11141-RA vs. NCBI nr
Match: XP_022934353.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 353.2 bits (905), Expect = 1.3e-93
Identity = 179/182 (98.35%), Postives = 180/182 (98.90%), Query Frame = 0


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKF+SAIVVVLCLCMAAKRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFVSAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE 180
           ALTVKAYGDLEKWVNIAITDVRTCGTELG ERPPLLTERNEVFIKLCEIALTISKIAQGE
Sbjct: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQGE 180

Query: 181 GN 183
           GN
Sbjct: 181 GN 182

[Close]

BLAST of Carg11141-RA vs. NCBI nr
Match: XP_022984074.1 (pectinesterase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 346.3 bits (887), Expect = 1.6e-91
Identity = 175/182 (96.15%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKFISAIVV+LCLCMA KRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE 180
           ALTVKAYGDLEKWVNIA+TDVRTCGTELG ERPPLLTERN+VFIKLCEIALTISKIAQGE
Sbjct: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQGE 180

Query: 181 GN 183
            N
Sbjct: 181 KN 182

[Close]

BLAST of Carg11141-RA vs. NCBI nr
Match: XP_023528768.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 341.3 bits (874), Expect = 5.2e-90
Identity = 172/177 (97.18%), Postives = 175/177 (98.87%), Query Frame = 0


Query: 6   AIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 65
           AIVVVLCLCMAAKRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA
Sbjct: 8   AIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 67

Query: 66  LVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVK 125
           LVALRFASSNASDISESIKVMFNDSTL+KPMVQEALLDCLDEYLDASQQLDDSIAALTVK
Sbjct: 68  LVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQQLDDSIAALTVK 127

Query: 126 AYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGEGN 183
           AYGDLEKWVNIA+TDVRTCGTELG ERPPLLTERN+VFIKLCEIALTISKIAQGEGN
Sbjct: 128 AYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQGEGN 184

[Close]

BLAST of Carg11141-RA vs. NCBI nr
Match: XP_038906317.1 (cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida])

HSP 1 Score: 249.2 bits (635), Expect = 2.7e-62
Identity = 127/183 (69.40%), Postives = 151/183 (82.51%), Query Frame = 0


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKF+SAI+ VLCL M A+R DA  P E+G+ +I+ MC+QT +K+LCV SLTSDPNS+PAD
Sbjct: 1   MKFVSAILFVLCLSMVAQRLDAASPAEDGLSMIDHMCAQTNYKDLCVISLTSDPNSFPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           K GLALVALRFASSNASDISESIKVM ND++L+KP VQ+ L DCLDEYLDASQQLDD+IA
Sbjct: 61  KMGLALVALRFASSNASDISESIKVMLNDTSLNKPSVQQGLFDCLDEYLDASQQLDDAIA 120

Query: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQ-G 180
           A+ VKAYGD+EKWV+ AI  VRTC  E    +P LL  +N+VFIKLCEIAL+ISK+A   
Sbjct: 121 AILVKAYGDVEKWVDAAIASVRTCENEFA-TKPSLLAPKNDVFIKLCEIALSISKVADTA 180

Query: 181 EGN 183
           EGN
Sbjct: 181 EGN 182

[Close]

BLAST of Carg11141-RA vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.2e-08
Identity = 42/156 (26.92%), Postives = 70/156 (44.87%), Query Frame = 0


Query: 28  EGMVLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMF 87
           +   L+   C+ T + ++CV +L +DP+S  AD  GL+ +A+  A++NAS  +++   + 
Sbjct: 57  QAAALVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALA 116

Query: 88  ND------STLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAITDV 147
           N       +      VQ  L  C  +Y DA   L  +  ++  + Y      V+      
Sbjct: 117 NGTAPLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYP 176

Query: 148 RTCGTELGRERPPL----LTERNEVFIKLCEIALTI 174
           + C     R+RP      L  R E   KLC +AL I
Sbjct: 177 QVCKALFRRQRPGAYPAELAAREEALNKLCSVALDI 212

[Close]

BLAST of Carg11141-RA vs. ExPASy TrEMBL
Match: A0A6J1F7F2 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.4e-94
Identity = 179/182 (98.35%), Postives = 180/182 (98.90%), Query Frame = 0


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKF+SAIVVVLCLCMAAKRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFVSAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE 180
           ALTVKAYGDLEKWVNIAITDVRTCGTELG ERPPLLTERNEVFIKLCEIALTISKIAQGE
Sbjct: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQGE 180

Query: 181 GN 183
           GN
Sbjct: 181 GN 182

[Close]

BLAST of Carg11141-RA vs. ExPASy TrEMBL
Match: A0A6J1J9E7 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 7.9e-92
Identity = 175/182 (96.15%), Postives = 178/182 (97.80%), Query Frame = 0


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKFISAIVV+LCLCMA KRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE 180
           ALTVKAYGDLEKWVNIA+TDVRTCGTELG ERPPLLTERN+VFIKLCEIALTISKIAQGE
Sbjct: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQGE 180

Query: 181 GN 183
            N
Sbjct: 181 KN 182

[Close]

BLAST of Carg11141-RA vs. ExPASy TrEMBL
Match: A0A0A0KY96 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 4.1e-56
Identity = 118/180 (65.56%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 1   MKFISAIVVVLCLCMAAKRHDATRPV-EEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPA 60
           MKF SAI++VLCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFFSAILLVLCLCMAPQRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60

Query: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLSKPMVQEALLDCLDEYLDASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S  ++P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120

Query: 121 IAALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQ 179
           IAA+  KAYGD+++WV +A+T+VRTC +     +P +LT RNE FIKLC+IAL+I+KIA+
Sbjct: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSF-PTKPSVLTPRNEEFIKLCDIALSITKIAE 179

[Close]

BLAST of Carg11141-RA vs. ExPASy TrEMBL
Match: A0A5D3E196 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001210 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 8.0e-52
Identity = 110/165 (66.67%), Postives = 136/165 (82.42%), Query Frame = 0


Query: 15  MAAKRHD-ATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFAS 74
           MAA+R D A++  +EG+  IE+MC+QT +K+LCV SLTSDPNS+PADK GLALVALR AS
Sbjct: 1   MAAQRLDAASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLAS 60

Query: 75  SNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKW 134
           SNASDISESIKVM N+++ ++P VQ+ L DCLDEYLDASQQLDDSIAA+  KAYGD+EKW
Sbjct: 61  SNASDISESIKVMLNETSQNEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEKW 120

Query: 135 VNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQ 179
           V+ A+ DVRTC       +P +LT RNE FIKLC+IAL+IS+IA+
Sbjct: 121 VHAAVADVRTCENSF-PTKPSVLTPRNEEFIKLCDIALSISRIAE 164

[Close]

BLAST of Carg11141-RA vs. ExPASy TrEMBL
Match: A0A6J1DPW6 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 8.2e-49
Identity = 112/176 (63.64%), Postives = 135/176 (76.70%), Query Frame = 0


Query: 6   AIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 65
           A++VV+ L +AA   +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+PADKTGLA
Sbjct: 8   AMLVVVWLSVAAAA-EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLA 67

Query: 66  LVALRFASSNASDISESIKVMFNDSTLS-KPMVQEALLDCLDEYLDASQQLDDSIAALTV 125
           LVALR  SSNASDISE IKVM ND+ L+ +P VQ+ L DCLDEYLDASQQLDDSIAAL V
Sbjct: 68  LVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLV 127

Query: 126 KAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQGE 181
           KAYGD++KWVN A+  VRTC       +P +LT RN+ FIKLC+ ALTI+ IA+ E
Sbjct: 128 KAYGDVDKWVNAAVAAVRTCEASFS-VKPSVLTPRNDNFIKLCQNALTIAHIAETE 181

[Close]

BLAST of Carg11141-RA vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.2e-22
Identity = 63/176 (35.80%), Postives = 103/176 (58.52%), Query Frame = 0


Query: 5   SAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNS--YPADKT 64
           SA V+ LC+ + +     T     GM  I ++C  +    LC+ S+TS P S  +     
Sbjct: 4   SAGVLFLCIVLIS---FVTGNANSGM--ISDLCKHSDDPNLCLSSITSRPESGEFAGTSN 63

Query: 65  GLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAAL 124
            + ++A+  AS+NAS  S  IK   ++  L +P +++ L DC  +Y DA +QLDDSI+A+
Sbjct: 64  QIEIIAISAASANASATSSYIKQKLSNEDL-EPAIEDTLEDCQKDYQDAVEQLDDSISAM 123

Query: 125 TVKAYGDLEKWVNIAITDVRTCGTELGRE--RPPLLTERNEVFIKLCEIALTISKI 177
              A+ D++ W++ AI+ + +CG+ LG        L++RNEVF+KLC+ AL I+K+
Sbjct: 124 LADAHTDVDVWLSAAISAIESCGSALGSRAGNDAELSQRNEVFLKLCKNALMINKM 173

[Close]

BLAST of Carg11141-RA vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 8.8e-19
Identity = 57/136 (41.91%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 43  KELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALL 102
           K  CV SLTS P +  A    L ++AL  ASSNASD S  IK       L +P +++ L 
Sbjct: 23  KAFCVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNL-EPALEDTLD 82

Query: 103 DCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAITDVRTCGTELGRE--RPPLLTERN 162
           DC   YLDA  QLDDS+AAL   ++ D++ W+N AI+D   C   L         L  RN
Sbjct: 83  DCSKNYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRN 142

Query: 163 EVFIKLCEIALTISKI 177
              +KLC+ AL I+ I
Sbjct: 143 TNLLKLCKDALLINTI 157

[Close]

BLAST of Carg11141-RA vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 43.9 bits (102), Expect = 1.6e-04
Identity = 43/172 (25.00%), Postives = 78/172 (45.35%), Query Frame = 0


Query: 4   ISAIVVVLCLCMAA-----KRHDATRPVEEG-----MVLIEEMCSQTKHKELCVDSLTSD 63
           IS+IV+V C+ + A      R ++ +P  E       V ++ +C  T HKE C ++L S 
Sbjct: 30  ISSIVLV-CIVVGAVVGTTARDNSKKPPTENNGEPISVSVKALCDVTLHKEKCFETLGSA 89

Query: 64  PNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQ 123
           PN+  +    L   A++   +  S + +     F++          A+  C++    A  
Sbjct: 90  PNASRSSPEELFKYAVKVTITELSKVLDG----FSNGEHMDNATSAAMGACVELIGLAVD 149

Query: 124 QLDDSIAALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIK 166
           QL++++ + ++K + DL  W++   T   TC   L     P LT   E  +K
Sbjct: 150 QLNETMTS-SLKNFDDLRTWLSSVGTYQETCMDALVEANKPSLTTFGENHLK 195

The following BLAST results are available for this feature:

BLAST of Carg11141-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581280.1	7.1e-95	100.00	Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. sororia] >KAG70...	[more]
XP_022934353.1	1.3e-93	98.35	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
XP_022984074.1	1.6e-91	96.15	pectinesterase inhibitor-like [Cucurbita maxima]	[more]
XP_023528768.1	5.2e-90	97.18	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_038906317.1	2.7e-62	69.40	cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida]	[more]

BLAST of Carg11141-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0J8J8	5.2e-08	26.92	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of Carg11141-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F7F2	6.4e-94	98.35	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4...	[more]
A0A6J1J9E7	7.9e-92	96.15	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 S...	[more]
A0A0A0KY96	4.1e-56	65.56	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 S...	[more]
A0A5D3E196	8.0e-52	66.67	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A6J1DPW6	8.2e-49	63.64	pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=...	[more]

BLAST of Carg11141-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55770.1	2.2e-22	35.80	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	8.8e-19	41.91	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G04960.1	1.6e-04	25.00	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..173 e-value: 1.2E-24 score: 97.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..173 e-value: 4.5E-28 score: 98.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..176 e-value: 8.5E-38 score: 127.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..176 e-value: 9.7E-36 score: 125.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..175
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 11..176
None	No IPR available	PANTHER	PTHR31080:SF1	OS05G0360900 PROTEIN	coord: 11..176
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 32..177 e-value: 3.36648E-45 score: 144.396

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg11141	Carg11141	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg11141-RA:1	Carg11141-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg11141-RA:cds	Carg11141-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg11141-RA	Carg11141-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity