Carg10289-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10289-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionEF hand calcium-binding family protein
LocationCarg_Chr10: 253572 .. 254060 (-)
Sequence length489
RNA-Seq ExpressionCarg10289-RA
SyntenyCarg10289-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGAATTGACCAAACATCCAAACTCGAGAAAATCCGAAATTTTCCAATCTCGAATCGAAATGGAAATCAACAGTTCGGCACAGTACGGTCGTGTGTTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGGTCGGATTGATGGGCGGAGAGCTGCTGCTGGGGGAAGCGGAGATGGCGGTGGAGTCGCTCGACTCGGACGGCGACGGGTTGCTGTGCGCGGGCGATTTCGAACGGCTTCTGGACGTGGGGGAGGAGGAGAAATTGAAGGATTTGAAAGAGGCGTTTGGTCTGTACGATTGCGAAGGTTGCGGGTTCATAACTCCGAAGAGCTTGAAGAGAATGCTGAGAAAATTGGGGGAATCGAAGTCCACAGCAGAGTGCAAGTTGATGATCGGTCAGTTTGATTTGAACGGGGATGGTTTGATTAGCTTTGAGGAGTTTCAGATAATGATGGCGTAA

mRNA sequence

ATGCCTGAATTGACCAAACATCCAAACTCGAGAAAATCCGAAATTTTCCAATCTCGAATCGAAATGGAAATCAACAGTTCGGCACAGTACGGTCGTGTGTTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGGTCGGATTGATGGGCGGAGAGCTGCTGCTGGGGGAAGCGGAGATGGCGGTGGAGTCGCTCGACTCGGACGGCGACGGGTTGCTGTGCGCGGGCGATTTCGAACGGCTTCTGGACGTGGGGGAGGAGGAGAAATTGAAGGATTTGAAAGAGGCGTTTGGTCTGTACGATTGCGAAGGTTGCGGGTTCATAACTCCGAAGAGCTTGAAGAGAATGCTGAGAAAATTGGGGGAATCGAAGTCCACAGCAGAGTGCAAGTTGATGATCGGTCAGTTTGATTTGAACGGGGATGGTTTGATTAGCTTTGAGGAGTTTCAGATAATGATGGCGTAA

Coding sequence (CDS)

ATGCCTGAATTGACCAAACATCCAAACTCGAGAAAATCCGAAATTTTCCAATCTCGAATCGAAATGGAAATCAACAGTTCGGCACAGTACGGTCGTGTGTTGAGTTACTTCGACGAAGATGGAGACGGCAAGATCTCGCCGTCGGAGCTGAGGAATCGGGTCGGATTGATGGGCGGAGAGCTGCTGCTGGGGGAAGCGGAGATGGCGGTGGAGTCGCTCGACTCGGACGGCGACGGGTTGCTGTGCGCGGGCGATTTCGAACGGCTTCTGGACGTGGGGGAGGAGGAGAAATTGAAGGATTTGAAAGAGGCGTTTGGTCTGTACGATTGCGAAGGTTGCGGGTTCATAACTCCGAAGAGCTTGAAGAGAATGCTGAGAAAATTGGGGGAATCGAAGTCCACAGCAGAGTGCAAGTTGATGATCGGTCAGTTTGATTTGAACGGGGATGGTTTGATTAGCTTTGAGGAGTTTCAGATAATGATGGCGTAA

Protein sequence

MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA
Homology
BLAST of Carg10289-RA vs. NCBI nr
Match: KAG7023071.1 (putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 324.7 bits (831), Expect = 4.5e-85
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 60
           MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE
Sbjct: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 60

Query: 61  LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS 120
           LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS
Sbjct: 61  LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS 120

Query: 121 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 163
           LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA
Sbjct: 121 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 162

BLAST of Carg10289-RA vs. NCBI nr
Match: XP_022921811.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 323.2 bits (827), Expect = 1.3e-84
Identity = 160/162 (98.77%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 60
           MPELTKHPNSRKSE+FQSRIEMEIN+SAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE
Sbjct: 4   MPELTKHPNSRKSEVFQSRIEMEINNSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 63

Query: 61  LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS 120
           LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS
Sbjct: 64  LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS 123

Query: 121 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 163
           LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA
Sbjct: 124 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 165

BLAST of Carg10289-RA vs. NCBI nr
Match: KAG6589392.1 (putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 284.3 bits (726), Expect = 6.8e-73
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 22  MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 81
           MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL
Sbjct: 1   MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 60

Query: 82  CAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 141
           CAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI
Sbjct: 61  CAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 120

Query: 142 GQFDLNGDGLISFEEFQIMMA 163
           GQFDLNGDGLISFEEFQIMMA
Sbjct: 121 GQFDLNGDGLISFEEFQIMMA 141

BLAST of Carg10289-RA vs. NCBI nr
Match: XP_023516478.1 (probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280.0 bits (715), Expect = 1.3e-71
Identity = 138/141 (97.87%), Postives = 140/141 (99.29%), Query Frame = 0

Query: 22  MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 81
           MEIN+SAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAV+SLDSDGDGLL
Sbjct: 1   MEINNSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVQSLDSDGDGLL 60

Query: 82  CAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 141
           CAGDFERLLDVGEEEKL DLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI
Sbjct: 61  CAGDFERLLDVGEEEKLNDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 120

Query: 142 GQFDLNGDGLISFEEFQIMMA 163
           GQFDLNGDGLISFEEFQIMMA
Sbjct: 121 GQFDLNGDGLISFEEFQIMMA 141

BLAST of Carg10289-RA vs. NCBI nr
Match: XP_038879778.1 (putative calcium-binding protein CML19 [Benincasa hispida])

HSP 1 Score: 278.1 bits (710), Expect = 4.8e-71
Identity = 141/167 (84.43%), Postives = 150/167 (89.82%), Query Frame = 0

Query: 2   PELTKHPNSRKS------EIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVG 61
           PELTK+PNS K+      +I QSRIEMEIN +AQY RVLSYFDEDGDGKISPSELRNR+G
Sbjct: 6   PELTKNPNSEKNPQNSFLKILQSRIEMEINCAAQYDRVLSYFDEDGDGKISPSELRNRLG 65

Query: 62  LMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGF 121
           LMGGELL  EAE AVESLDSDGDGLLCAGDFERLL+VGEEEKLKDLKEAF LYDCEGCGF
Sbjct: 66  LMGGELLQAEAEAAVESLDSDGDGLLCAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGF 125

Query: 122 ITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 163
           ITPK+LK+MLRKLGE KST ECKLMI +FDLNGDGLISFEEFQIMMA
Sbjct: 126 ITPKNLKKMLRKLGERKSTEECKLMIRRFDLNGDGLISFEEFQIMMA 172

BLAST of Carg10289-RA vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 8.1e-29
Identity = 73/143 (51.05%), Postives = 98/143 (68.53%), Query Frame = 0

Query: 24  INSSAQYGRVLSYFDEDGDGKISPSELRNRV-GLMGGELLLGEAEMAVESLDSDGDGLLC 83
           + ++A++ RV S FD D DGKIS +ELR  +   +G ++   EAE  V S D+D DGLL 
Sbjct: 2   VAATAEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLD 61

Query: 84  AGDFERL---LDVG-EEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECK 143
             +F +L   L++G EEE+ + L EAF +Y+ EG G ITP SLKRML KLG  +   EC+
Sbjct: 62  EEEFTKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQ 121

Query: 144 LMIGQFDLNGDGLISFEEFQIMM 162
            MI +FDL+GDG+ISFEEF+IMM
Sbjct: 122 TMICRFDLDGDGVISFEEFKIMM 144

BLAST of Carg10289-RA vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-28
Identity = 68/142 (47.89%), Postives = 95/142 (66.90%), Query Frame = 0

Query: 26  SSAQYGRVLSYFDEDGDGKISPSELRNRV-GLMGGELLLGEAEMAVESLDSDGDGLLCAG 85
           S  +   + + FD DGDG+IS +ELR  +   +G E+   EA   V S+D+DGDGLLC  
Sbjct: 8   SPCESSALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEA 67

Query: 86  DFERLLDVGEEEKLKD-----LKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKL 145
           +F RL+   E E+  +     L+EAFG+Y+ EG G ITP SL+RMLR+LG  +   +C+ 
Sbjct: 68  EFVRLVQAAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRA 127

Query: 146 MIGQFDLNGDGLISFEEFQIMM 162
           MI +FDLNGDG++SF+EF+IMM
Sbjct: 128 MICRFDLNGDGVLSFDEFKIMM 149

BLAST of Carg10289-RA vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.3e-28
Identity = 67/130 (51.54%), Postives = 89/130 (68.46%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDV 92
           V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   +F +L+ V
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIKV 106

Query: 93  -GEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGL 152
             EEEK  +LK AF LY  EG   ITP+SLK ML+KLGES++T +C++MI  FDLN DG+
Sbjct: 107 DDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADGV 166

Query: 153 ISFEEFQIMM 162
           +SF+EF +MM
Sbjct: 167 LSFDEFALMM 176

BLAST of Carg10289-RA vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.4e-25
Identity = 72/174 (41.38%), Postives = 101/174 (58.05%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYG----------RVLSYFDEDGDGKISPSEL 60
           +  L K  +S++SE  ++  +    SS   G           V  Y D + DGKIS  EL
Sbjct: 11  LSRLYKKVSSKRSESSRNLEDESRTSSNSSGSSSLNVNELRTVFDYMDANSDGKISGEEL 70

Query: 61  RNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLL---DVGEEEKLKDLKEAFGL 120
           ++ V L+GG L   E E  V++ D DGDG +   +F +L+   D  +EE+ K+LKEAFG+
Sbjct: 71  QSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEGEDGSDEERRKELKEAFGM 130

Query: 121 YDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMM 162
           Y  EG  FIT  SL+R L +LGES +   CK+MI  FD N DG++SF+EF +MM
Sbjct: 131 YVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDDGVLSFDEFVLMM 184

BLAST of Carg10289-RA vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.2e-25
Identity = 71/147 (48.30%), Postives = 93/147 (63.27%), Query Frame = 0

Query: 26  SSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESL-DSDGDGLLCAG 85
           +S ++ RV   FD+DGDGKIS +ELR  V    GE +  E   A+ +L D+DGDGLL   
Sbjct: 3   ASDEFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEE 62

Query: 86  DFERLL-----DVGEEEKLKD-----LKEAFGLYDCEGCGFITPKSLKRMLRKLGESKST 145
           +F RL+     D  EEE   D     L+EAF +Y+ EG G ITP SLK ML KLG     
Sbjct: 63  EFVRLVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDV 122

Query: 146 AECKLMIGQFDLNGDGLISFEEFQIMM 162
           AEC+ MI +FD+NGDG+++F+EF+ MM
Sbjct: 123 AECQAMICRFDMNGDGVLTFDEFKTMM 149

BLAST of Carg10289-RA vs. ExPASy TrEMBL
Match: A0A6J1E2E7 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111429954 PE=4 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 6.4e-85
Identity = 160/162 (98.77%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 60
           MPELTKHPNSRKSE+FQSRIEMEIN+SAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE
Sbjct: 4   MPELTKHPNSRKSEVFQSRIEMEINNSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGE 63

Query: 61  LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS 120
           LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS
Sbjct: 64  LLLGEAEMAVESLDSDGDGLLCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKS 123

Query: 121 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 163
           LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA
Sbjct: 124 LKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMMA 165

BLAST of Carg10289-RA vs. ExPASy TrEMBL
Match: A0A6J1JE84 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111484995 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.4e-69
Identity = 134/141 (95.04%), Postives = 136/141 (96.45%), Query Frame = 0

Query: 22  MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 81
           MEINSSA YGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL
Sbjct: 1   MEINSSAHYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 60

Query: 82  CAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 141
           CAGDFERLL+VGEEEKLKDLKEAF LYDCEGCGFITPKSLK+MLRKLGE KSTAECKLMI
Sbjct: 61  CAGDFERLLEVGEEEKLKDLKEAFALYDCEGCGFITPKSLKKMLRKLGERKSTAECKLMI 120

Query: 142 GQFDLNGDGLISFEEFQIMMA 163
           G FDLNGDGLISFEEFQ MMA
Sbjct: 121 GVFDLNGDGLISFEEFQKMMA 141

BLAST of Carg10289-RA vs. ExPASy TrEMBL
Match: A0A6J1EJM6 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111433200 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.4e-62
Identity = 122/141 (86.52%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 22  MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 81
           MEI S+A Y RVL YFDEDGDGKISPSELRNR+GL+GGELL  EAE AVESLDSDGDGLL
Sbjct: 1   MEITSAAHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEAAVESLDSDGDGLL 60

Query: 82  CAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 141
           C GD ERLLDVGEEEKLKDL+EAFGLYD EGCGFITPKSLKRML+KLGE+KSTAECK+MI
Sbjct: 61  CVGDIERLLDVGEEEKLKDLREAFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120

Query: 142 GQFDLNGDGLISFEEFQIMMA 163
            +FDLNGDGLISFEEFQIMMA
Sbjct: 121 RRFDLNGDGLISFEEFQIMMA 141

BLAST of Carg10289-RA vs. ExPASy TrEMBL
Match: A0A6J1HRH8 (probable calcium-binding protein CML31 OS=Cucurbita maxima OX=3661 GN=LOC111466049 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 7.6e-62
Identity = 121/141 (85.82%), Postives = 130/141 (92.20%), Query Frame = 0

Query: 22  MEINSSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLL 81
           MEI S+A Y RVL YFDEDGDGKISPSELRNR+GL+GGELL  EAE AVESLDSDGDGLL
Sbjct: 1   MEITSAAHYDRVLRYFDEDGDGKISPSELRNRLGLIGGELLPAEAEAAVESLDSDGDGLL 60

Query: 82  CAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMI 141
           C GD ERLLDVGEEEKLKDL+EAFGLYD EGCGFITPKSLKRML+KLGE+KSTAECK+MI
Sbjct: 61  CVGDIERLLDVGEEEKLKDLREAFGLYDAEGCGFITPKSLKRMLKKLGETKSTAECKVMI 120

Query: 142 GQFDLNGDGLISFEEFQIMMA 163
            +FDLNGDGLISF+EFQIMMA
Sbjct: 121 RRFDLNGDGLISFDEFQIMMA 141

BLAST of Carg10289-RA vs. ExPASy TrEMBL
Match: A0A5A7URJ2 (Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00610 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.7e-61
Identity = 123/142 (86.62%), Postives = 130/142 (91.55%), Query Frame = 0

Query: 22  MEIN-SSAQYGRVLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGL 81
           MEIN S AQY RVLSYFDEDGDGKISPSELRNR+GL+GGELL  EAE AVESLDSDGDGL
Sbjct: 1   MEINKSGAQYDRVLSYFDEDGDGKISPSELRNRLGLIGGELLQAEAEAAVESLDSDGDGL 60

Query: 82  LCAGDFERLLDVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLM 141
           LCAGD ERLL+VGEEEKLKDLKEAF LYD EGCGFITPK+LK+MLRKLGE KST ECK+M
Sbjct: 61  LCAGDIERLLEVGEEEKLKDLKEAFALYDSEGCGFITPKNLKKMLRKLGERKSTEECKMM 120

Query: 142 IGQFDLNGDGLISFEEFQIMMA 163
           I +FDLNGDGLISFEEFQIMMA
Sbjct: 121 IRRFDLNGDGLISFEEFQIMMA 142

BLAST of Carg10289-RA vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.7e-29
Identity = 67/130 (51.54%), Postives = 89/130 (68.46%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDV 92
           V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   +F +L+ V
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIKV 106

Query: 93  -GEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGL 152
             EEEK  +LK AF LY  EG   ITP+SLK ML+KLGES++T +C++MI  FDLN DG+
Sbjct: 107 DDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADGV 166

Query: 153 ISFEEFQIMM 162
           +SF+EF +MM
Sbjct: 167 LSFDEFALMM 176

BLAST of Carg10289-RA vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.7e-29
Identity = 67/130 (51.54%), Postives = 89/130 (68.46%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDV 92
           V SY D + DG+ISP EL+     +G +L   EA  AV   D+DGDG+L   +F +L+ V
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIKV 106

Query: 93  -GEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGL 152
             EEEK  +LK AF LY  EG   ITP+SLK ML+KLGES++T +C++MI  FDLN DG+
Sbjct: 107 DDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADGV 166

Query: 153 ISFEEFQIMM 162
           +SF+EF +MM
Sbjct: 167 LSFDEFALMM 176

BLAST of Carg10289-RA vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 117.5 bits (293), Expect = 1.0e-26
Identity = 72/174 (41.38%), Postives = 101/174 (58.05%), Query Frame = 0

Query: 1   MPELTKHPNSRKSEIFQSRIEMEINSSAQYG----------RVLSYFDEDGDGKISPSEL 60
           +  L K  +S++SE  ++  +    SS   G           V  Y D + DGKIS  EL
Sbjct: 11  LSRLYKKVSSKRSESSRNLEDESRTSSNSSGSSSLNVNELRTVFDYMDANSDGKISGEEL 70

Query: 61  RNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLL---DVGEEEKLKDLKEAFGL 120
           ++ V L+GG L   E E  V++ D DGDG +   +F +L+   D  +EE+ K+LKEAFG+
Sbjct: 71  QSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEGEDGSDEERRKELKEAFGM 130

Query: 121 YDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGLISFEEFQIMM 162
           Y  EG  FIT  SL+R L +LGES +   CK+MI  FD N DG++SF+EF +MM
Sbjct: 131 YVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDDGVLSFDEFVLMM 184

BLAST of Carg10289-RA vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 117.1 bits (292), Expect = 1.3e-26
Identity = 64/130 (49.23%), Postives = 86/130 (66.15%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLLDV 92
           V SY D + DG+ISP EL+     +G +LL           D+DGDG+L   +F +L+ V
Sbjct: 47  VFSYMDANRDGRISPEELQKSFMTLGEQLL----------SDTDGDGMLDFEEFSQLIKV 106

Query: 93  -GEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNGDGL 152
             EEEK  +LK AF LY  EG   ITP+SLK ML+KLGES++T +C++MI  FDLN DG+
Sbjct: 107 DDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNADGV 166

Query: 153 ISFEEFQIMM 162
           +SF+EF +MM
Sbjct: 167 LSFDEFALMM 166

BLAST of Carg10289-RA vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 115.9 bits (289), Expect = 3.0e-26
Identity = 62/133 (46.62%), Postives = 86/133 (64.66%), Query Frame = 0

Query: 33  VLSYFDEDGDGKISPSELRNRVGLMGGELLLGEAEMAVESLDSDGDGLLCAGDFERLL-- 92
           V +Y D + DG+IS  EL+     +G ++   EAE AV+  D DGDG+L   +F  L+  
Sbjct: 26  VFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLIKG 85

Query: 93  --DVGEEEKLKDLKEAFGLYDCEGCGFITPKSLKRMLRKLGESKSTAECKLMIGQFDLNG 152
             +  EEEK + + EAF +Y  +G   ITP SLK ML KLGES++T +CK+MI  FDLN 
Sbjct: 86  NDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDLNA 145

Query: 153 DGLISFEEFQIMM 162
           DG++SF+EF +MM
Sbjct: 146 DGVLSFDEFALMM 158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023071.14.5e-85100.00putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022921811.11.3e-8498.77putative calcium-binding protein CML19 [Cucurbita moschata][more]
KAG6589392.16.8e-73100.00putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023516478.11.3e-7197.87probable calcium-binding protein CML31 [Cucurbita pepo subsp. pepo][more]
XP_038879778.14.8e-7184.43putative calcium-binding protein CML19 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8RYJ88.1e-2951.05Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8RYK01.8e-2847.89Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SRE65.3e-2851.54Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1[more]
Q9FIH91.4e-2541.38Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1[more]
Q8RYJ93.2e-2548.30Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A6J1E2E76.4e-8598.77putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11142... [more]
A0A6J1JE846.4e-6995.04putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114849... [more]
A0A6J1EJM63.4e-6286.52putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1HRH87.6e-6285.82probable calcium-binding protein CML31 OS=Cucurbita maxima OX=3661 GN=LOC1114660... [more]
A0A5A7URJ23.7e-6186.62Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT1G76650.13.7e-2951.54calmodulin-like 38 [more]
AT1G76650.33.7e-2951.54calmodulin-like 38 [more]
AT5G42380.11.0e-2641.38calmodulin like 37 [more]
AT1G76650.21.3e-2649.23calmodulin-like 38 [more]
AT1G76640.13.0e-2646.62Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 65..93
e-value: 59.0
score: 3.5
coord: 100..128
e-value: 0.27
score: 20.3
coord: 136..162
e-value: 1.1
score: 17.3
coord: 29..57
e-value: 1.2
score: 17.2
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 99..161
e-value: 2.7E-13
score: 50.2
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 35..52
e-value: 3.3E-5
score: 23.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 25..60
score: 11.249643
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 96..131
score: 10.775421
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 132..162
score: 12.672309
IPR002048EF-hand domainCDDcd00051EFhcoord: 100..162
e-value: 6.70092E-14
score: 61.0245
NoneNo IPR availableGENE3D1.10.238.10coord: 91..162
e-value: 5.8E-21
score: 76.5
NoneNo IPR availableGENE3D1.10.238.10coord: 1..90
e-value: 2.7E-9
score: 39.1
NoneNo IPR availablePANTHERPTHR10891:SF870EF HAND CALCIUM-BINDING FAMILY PROTEINcoord: 26..162
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 26..162
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 38..50
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 145..157
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 25..161

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg10289Carg10289gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10289-RA:1Carg10289-RA:1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10289-RA:cdsCarg10289-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg10289-RACarg10289-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding