Carg10178-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10178-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr17: 273496 .. 275020 (-)
Sequence length	1353
RNA-Seq Expression	Carg10178-RA
Synteny	Carg10178-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGATATCCGCCAAAACCGTCTTCCTCCGCCGCAGCCACTGCCGCCACCGCCGGTCGTGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCAACCATGCAAATCATTGTCAACTTTGATATCACTCCAAATTATACACTCAAAAGGCTCATAGTCTCTTGGAAAATGAATGAATCTTGCTCCTCCGCCTCCTCCTCGTCGCCCCGACCGCCATCAATGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCGTTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCAGCAGTCGGAGCGAAACCCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAATCGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTGTTAATCTATCGATCGCACGTGGTTAATAAATATAAAAATTTCGGTCGATGAAATATGTTATGATATATTCAATCTTTTATTTATATTCTTAATAGGTATTATCTCAATTTCCATTTTCTAAAATCGAGAAGCTATTTGAGTTACTAACGAGACCAGAAGCCATCAAGTCAATGGCGATCGTTTTACAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAATTACTCCAACAAATATCCAAAAAGGGATATGATTATAACTCTTTATCGAAACATCATGCCATTGACTTGTTCAAGTCAATATTGGAGCTCGCGTCCGATGAGATAATCAAGAAAGCAAGTACAGTTAAGATGACAGACTCGTCCATAGCTACACAGTGGTATGACATTGTTCAAGCACGAAATTACTTTAGTTGGACGTTTCTCACTAAATCTTTTTTTTTTCTAGTCGATACGGGACGTTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTATTGATCGAAGCTGGTGCTGTTTGCATCATGATCGACCTTCTTCCTGACTCGAACCGATCGAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCATATCATGGGAATAGCGACGGTGACGAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAA

mRNA sequence

ATGGAGGATATCCGCCAAAACCGTCTTCCTCCGCCGCAGCCACTGCCGCCACCGCCGGTCGTGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCAACCATGCAAATCATTGTCAACTTTGATATCACTCCAAATTATACACTCAAAAGGCTCATAGTCTCTTGGAAAATGAATGAATCTTGCTCCTCCGCCTCCTCCTCGTCGCCCCGACCGCCATCAATGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCGTTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCAGCAGTCGGAGCGAAACCCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAATCGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCATTTTCTAAAATCGAGAAGCTATTTGAGTTACTAACGAGACCAGAAGCCATCAAGTCAATGGCGATCGTTTTACAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAATTACTCCAACAAATATCCAAAAAGGGATATGATTATAACTCTTTATCGAAACATCATGCCATTGACTTGTTCAAGTCAATATTGGAGCTCGCGTCCGATGAGATAATCAAGAAAGCAAGTACAGTTAAGATGACAGACTCGTCCATAGCTACACATCGATACGGGACGTTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTATTGATCGAAGCTGGTGCTGTTTGCATCATGATCGACCTTCTTCCTGACTCGAACCGATCGAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCATATCATGGGAATAGCGACGGTGACGAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAA

Coding sequence (CDS)

ATGGAGGATATCCGCCAAAACCGTCTTCCTCCGCCGCAGCCACTGCCGCCACCGCCGGTCGTGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCAACCATGCAAATCATTGTCAACTTTGATATCACTCCAAATTATACACTCAAAAGGCTCATAGTCTCTTGGAAAATGAATGAATCTTGCTCCTCCGCCTCCTCCTCGTCGCCCCGACCGCCATCAATGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCGTTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCAGCAGTCGGAGCGAAACCCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAATCGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCATTTTCTAAAATCGAGAAGCTATTTGAGTTACTAACGAGACCAGAAGCCATCAAGTCAATGGCGATCGTTTTACAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAATTACTCCAACAAATATCCAAAAAGGGATATGATTATAACTCTTTATCGAAACATCATGCCATTGACTTGTTCAAGTCAATATTGGAGCTCGCGTCCGATGAGATAATCAAGAAAGCAAGTACAGTTAAGATGACAGACTCGTCCATAGCTACACATCGATACGGGACGTTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTATTGATCGAAGCTGGTGCTGTTTGCATCATGATCGACCTTCTTCCTGACTCGAACCGATCGAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCATATCATGGGAATAGCGACGGTGACGAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAA

Protein sequence

MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN

Homology

[Close]

BLAST of Carg10178-RA vs. NCBI nr
Match: KAG7013396.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.4 bits (2284), Expect = 4.1e-253
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIM 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIM
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIM 300

Query: 301 ESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIAT 360
           ESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIAT
Sbjct: 301 ESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIAT 360

Query: 361 VTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEK 420
           VTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEK
Sbjct: 361 VTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEK 420

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           AMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 AMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 450

[Close]

BLAST of Carg10178-RA vs. NCBI nr
Match: KAG6574816.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 876.7 bits (2264), Expect = 8.5e-251
Identity = 449/451 (99.56%), Postives = 449/451 (99.56%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIAT  RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Carg10178-RA vs. NCBI nr
Match: XP_022958929.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 875.5 bits (2261), Expect = 1.9e-250
Identity = 447/451 (99.11%), Postives = 449/451 (99.56%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCSSASSSSPRPPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDV AVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEM+GCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Carg10178-RA vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 863.2 bits (2229), Expect = 9.8e-247
Identity = 441/451 (97.78%), Postives = 445/451 (98.67%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP QPLPPPP VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCSSASSSSP PPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL+RPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKASTVKMTDSSIAT  RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Carg10178-RA vs. NCBI nr
Match: XP_023006772.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 833.6 bits (2152), Expect = 8.3e-238
Identity = 427/451 (94.68%), Postives = 435/451 (96.45%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQ    PP  EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQ----PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCS ASSSSPRPPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAV AK IFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL++ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKAST+K TDSSI T  RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

[Close]

BLAST of Carg10178-RA vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.5e-45
Identity = 134/425 (31.53%), Postives = 210/425 (49.41%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IPP+F CPIS+E+M DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN+
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNH 70

Query: 83  TLKRLIVSW-KMNESCSSASSSSPRPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMAI 142
           TL+RLI SW  +N S       +PRPP  + +    +  +      +   LK+LR +++ 
Sbjct: 71  TLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSE 130

Query: 143 DDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANFEACEEALGVLSQFPFSK--IE 202
           +       K     A   E L  +V       S++  N    +EAL +L     S+  ++
Sbjct: 131 N----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLK 190

Query: 203 KLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLSKHHAIDLFKSIL 262
            L         +KS+  ++QRG  E R YA  LL+ I +      S++     ++F  ++
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVV 250

Query: 263 ELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIMESSKKCRLLLIEAGAVCIM 322
           ++  D I +KA+                   +A+ +L+ I    +  R   +EAG + ++
Sbjct: 251 QILDDRISQKAT------------------KAAMHILVNICPWGRN-RHKAVEAGVISVI 310

Query: 323 IDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNTATKI 382
           I+LL D    S R   E  + VL LL  CAEGR     H   IA V KK +  S TA+  
Sbjct: 311 IELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDR 370

Query: 383 GVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWRR 440
            V++L  +  +  +  +L EM+  G V KL  +L        TKEKA +++KLH   W+ 
Sbjct: 371 AVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKD 402

[Close]

BLAST of Carg10178-RA vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 6.1e-42
Identity = 131/451 (29.05%), Postives = 224/451 (49.67%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IP +F CPIS+++M DPV +STG++++R +IEKW F+  K +CP T Q+I   D+TPN+
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNH 65

Query: 83  TLKRLIVSW-KMNESCSSASSSSPRPPSMEYDDVVALLKNIELSPF-KASSLKKLRLLMA 142
           TL+RLI SW  +N S       +P+PP  +  ++  L+K    S   +   LK+LR +++
Sbjct: 66  TLRRLIQSWCTLNASYGIERIPTPKPPICK-SEIEKLIKESSSSHLNQVKCLKRLRQIVS 125

Query: 143 ID-------DVAAVGAKPIFVQANGFEVLVGLVTQIA------ICESSNFAN-FEA---- 202
            +       + A V      + +N  +      + ++      +C+S+   N F++    
Sbjct: 126 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 185

Query: 203 CEEALGVLSQFPFSK--IEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 262
            +EAL VL     S+  ++ L         +K++  ++QRG  E R YA  LL+++ +  
Sbjct: 186 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 245

Query: 263 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIM 322
                +      +LF  ++++  D+I  KA+                   SA+++L+   
Sbjct: 246 DPMQIILLER--ELFGEVIQILHDQISHKAT------------------RSAMQILVITC 305

Query: 323 ESSKKCRLLLIEAGAVCIMIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIM 382
              +  R   +E G + ++I+LL D    S R   E  + VL +L  CAEGR     H  
Sbjct: 306 PWGRN-RHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGA 365

Query: 383 GIATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSS 442
            IA V+KK +  S   ++  V++L  +  +  +  +L+EM+  G V KL  +L      +
Sbjct: 366 AIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGN 425

Query: 443 STKEKAMKIMKLHGSFWRRFPCFPYEFRDYF 447
            TKEKA +++KLH   WR  PC P    D +
Sbjct: 426 KTKEKAKELLKLHARVWRESPCVPRNLYDSY 433

[Close]

BLAST of Carg10178-RA vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.2e-35
Identity = 128/437 (29.29%), Postives = 214/437 (48.97%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ-IIVNFDITPN 82
           +IP YF CPIS+E+M DPVT  +G++++R NI KW       +CP T Q + ++ D+TPN
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPN 68

Query: 83  YTLKRLIVSWKM-NESCSSASSSSPRPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 142
           + L+RLI  W + NE+      S+PR P  +  +VV  +KN  L  F   +L +   L  
Sbjct: 69  HMLRRLIQHWCVENETRGVVRISTPRVPPGKL-NVVEEIKN--LKKFGQEALGREETLQK 128

Query: 143 IDDVAAVG-AKPIFVQANGFEVLVGLVTQIAICESSNFANFEACEEALGVLSQFPFSKIE 202
           ++ +A  G  + +  +    + L+  V +    +       +  +E+L +L        +
Sbjct: 129 LEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSND 188

Query: 203 KLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLSKHHAIDLFKSIL 262
               L+     ++S+  VL +     + Y + LL+ +++  Y  + + +    ++FK I+
Sbjct: 189 AKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKGII 248

Query: 263 ELASD---EIIKKASTVKMTDSSIATHRYGTLFSSALE-----------LLIEIMESS-- 322
               D    + + + TV+ T  S +    G    S L+            L+ ++E+S  
Sbjct: 249 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 308

Query: 323 KKCRLLLIEAGAVCIMIDLLPDSNRSK--CEKILHVLKLLSDCAEGRLAMAEHIMGIATV 382
            + R LL++ GAV  +I+L       K   E +L VL  L  CA GR  +  H  GIA V
Sbjct: 309 SRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVV 368

Query: 383 TKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEK 438
           TK+ +  S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D    + KEK
Sbjct: 369 TKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD-CGLNLKEK 428

[Close]

BLAST of Carg10178-RA vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.3e-28
Identity = 126/441 (28.57%), Postives = 205/441 (46.49%), Query Frame = 0


Query: 12  PQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ 71
           P+ + P  +   IP +FRCPIS+E+M DPVT+ TG +++RA+IE W    N  TCP T  
Sbjct: 2   PRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRA 61

Query: 72  IIVNFDITPNYTLKRLIVSWKM-NESCSSASSSSPRPPSMEYDDVVALLKNIELSP---- 131
            + +F + PN+TL+RLI  W + N S       +P+ P+ +   V ALL           
Sbjct: 62  PLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPA-DPTSVRALLSQASAITGTHV 121

Query: 132 ---FKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANFEAC 191
               +A++L++LR      D   V    +    N  E+L+ ++        S   + E  
Sbjct: 122 SVRSRAAALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELV 181

Query: 192 EEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQIS--KKGY 251
            E+L +L   P ++  +   + + P  ++ +  +L   SIE R  A  L++ +S   K  
Sbjct: 182 SESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 241

Query: 252 DYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYG--TLFSSALELLIEI 311
           D    S  ++  +F+ +L+L  + I           SS    + G  TLF+         
Sbjct: 242 DLKG-SISNSESVFEGVLDLLRNPI-----------SSRRALKIGIKTLFA--------- 301

Query: 312 MESSKKCRLLLIEAGAVCIMID-LLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 371
           + S K  R + I AGA  I+ID L  D +R   E+ L  ++LL    EG  A  EH + +
Sbjct: 302 LCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTV 361

Query: 372 ATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 431
             + K  +  S+ AT+     L  +C     ER  EE  G G V +L+ ++  +  +   
Sbjct: 362 PLLVKTILRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE-CTERA 406

Query: 432 KEKAMKIMKLHGSFWRRFPCF 439
           K+KA K++KL    W  +  F
Sbjct: 422 KKKAQKLLKLLRDSWPDYNSF 406

[Close]

BLAST of Carg10178-RA vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 7.3e-27
Identity = 110/401 (27.43%), Postives = 191/401 (47.63%), Query Frame = 0


Query: 21  VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITP 80
           +  +P +F+CPIS++VM  PV++STGV+++R +I++W    N  TCPATMQI+ N +  P
Sbjct: 8   ITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVP 67

Query: 81  NYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 140
           N TL RLI  W  +     A S SP   +   D++ A ++   +     S  K LR    
Sbjct: 68  NLTLHRLIDHWS-DSINRRADSESPESDTPTRDEINAAIERFRIENDARS--KILRFARE 127

Query: 141 IDDVAA-VGAKPIFVQANGFEVLVGLVTQIAICESSNFANFE--ACEEALGVLSQ----- 200
            D+    +  K  FV      +LV L++     +S NF++ +     EA+ +LS      
Sbjct: 128 SDENREFLAGKDDFV-----AMLVDLIS-----DSRNFSDSQLLLVGEAVKILSMIRRKI 187

Query: 201 FPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLSKHHAI 260
           F   ++  L  L    + + S  ++++RG+               K   D +++ +  A+
Sbjct: 188 FDRRRLSNLI-LTNGGDCLTSFFLLIKRGN--------------PKLKIDCSAVLEFIAV 247

Query: 261 DLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIMESSKKCRLLLIE 320
           D    ++    + ++ +   +  +DS        +L  + L LLI I  SSK+ +L LI 
Sbjct: 248 DAESKLIIAKGEGLVTEIIKLISSDSD------SSLIEANLSLLIAI-ASSKRVKLALIR 307

Query: 321 AGAVCIMIDLL--PDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMSTSNT 380
              V  +  LL  P ++ S  EK L +L+ +S C EGR  + + +     V K M  S  
Sbjct: 308 EKLVTKLTSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMKVSTA 367

Query: 381 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGD 412
           AT+  V +LW +C     ++  + ++    V K++ +L  +
Sbjct: 368 ATEHAVTVLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

[Close]

BLAST of Carg10178-RA vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 9.2e-251
Identity = 447/451 (99.11%), Postives = 449/451 (99.56%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCSSASSSSPRPPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDV AVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEM+GCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Carg10178-RA vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.0e-238
Identity = 427/451 (94.68%), Postives = 435/451 (96.45%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQ    PP  EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQ----PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCS ASSSSPRPPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAV AK IFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL++ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKAST+K TDSSI T  RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

[Close]

BLAST of Carg10178-RA vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.0e-210
Identity = 387/454 (85.24%), Postives = 411/454 (90.53%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PPP    P P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPPPH--APTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKM-NESCSSASSSSPRPPSMEYDDVVA-L 120
           YNKKTCPATMQ IVNFDITPNYTLKRLI+SWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLP-SSSMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFEVL+G+VTQ+AICESS+FA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240
           NFEACEEALGVLSQFPFSK EK FELL+ PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS +K TDSSI TH RYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGIL-NGDGRSSS 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GIL NGDGR SS
Sbjct: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SS 420

Query: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           TKEKA K+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450

[Close]

BLAST of Carg10178-RA vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.5e-205
Identity = 377/453 (83.22%), Postives = 403/453 (88.96%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQ-PLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP   P PPP    D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVA-L 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLI+SWKM ESCSS S       SMEY DVVA L
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLP--SSSMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFE+LV +VTQ+AICE S+FA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240
           NFEACEEALGVLSQFPFSK EK FELL+ PEAIKSMAI+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS +K  DSS  T  RYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKIL++LKLLS+CAEGR AM EH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420
           IATVTKKMS SNTATKIG+KILW IC+YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 420

Query: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450

[Close]

BLAST of Carg10178-RA vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.8e-182
Identity = 346/453 (76.38%), Postives = 372/453 (82.12%), Query Frame = 0


Query: 1   MEDIRQNRLPPPQ-PLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP   P PPP    D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVA-L 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLI+SWKM ESCSS S       SMEY DVVA L
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLP--SSSMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFE+LV +VTQ+AICE S+F 
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFT 180

Query: 181 NFEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240
           NFEACEEALGVLSQFPFSK EK FELL+ PEAIKSMAI+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS +K TDSS  T  RYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKIL++LKLLS+CAEG          
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG---------- 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420
                                     +YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 --------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 414

Query: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KEKAMK+MKLHGSFWR+FPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKVMKLHGSFWRKFPCFPYEYRDYLKLIN 414

[Close]

BLAST of Carg10178-RA vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 184.1 bits (466), Expect = 2.5e-46
Identity = 134/425 (31.53%), Postives = 210/425 (49.41%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IPP+F CPIS+E+M DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN+
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNH 70

Query: 83  TLKRLIVSW-KMNESCSSASSSSPRPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMAI 142
           TL+RLI SW  +N S       +PRPP  + +    +  +      +   LK+LR +++ 
Sbjct: 71  TLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSE 130

Query: 143 DDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANFEACEEALGVLSQFPFSK--IE 202
           +       K     A   E L  +V       S++  N    +EAL +L     S+  ++
Sbjct: 131 N----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLK 190

Query: 203 KLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLSKHHAIDLFKSIL 262
            L         +KS+  ++QRG  E R YA  LL+ I +      S++     ++F  ++
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVV 250

Query: 263 ELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIMESSKKCRLLLIEAGAVCIM 322
           ++  D I +KA+                   +A+ +L+ I    +  R   +EAG + ++
Sbjct: 251 QILDDRISQKAT------------------KAAMHILVNICPWGRN-RHKAVEAGVISVI 310

Query: 323 IDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNTATKI 382
           I+LL D    S R   E  + VL LL  CAEGR     H   IA V KK +  S TA+  
Sbjct: 311 IELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDR 370

Query: 383 GVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWRR 440
            V++L  +  +  +  +L EM+  G V KL  +L        TKEKA +++KLH   W+ 
Sbjct: 371 AVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKD 402

[Close]

BLAST of Carg10178-RA vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 173.3 bits (438), Expect = 4.3e-43
Identity = 131/451 (29.05%), Postives = 224/451 (49.67%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IP +F CPIS+++M DPV +STG++++R +IEKW F+  K +CP T Q+I   D+TPN+
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNH 65

Query: 83  TLKRLIVSW-KMNESCSSASSSSPRPPSMEYDDVVALLKNIELSPF-KASSLKKLRLLMA 142
           TL+RLI SW  +N S       +P+PP  +  ++  L+K    S   +   LK+LR +++
Sbjct: 66  TLRRLIQSWCTLNASYGIERIPTPKPPICK-SEIEKLIKESSSSHLNQVKCLKRLRQIVS 125

Query: 143 ID-------DVAAVGAKPIFVQANGFEVLVGLVTQIA------ICESSNFAN-FEA---- 202
            +       + A V      + +N  +      + ++      +C+S+   N F++    
Sbjct: 126 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 185

Query: 203 CEEALGVLSQFPFSK--IEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 262
            +EAL VL     S+  ++ L         +K++  ++QRG  E R YA  LL+++ +  
Sbjct: 186 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 245

Query: 263 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIM 322
                +      +LF  ++++  D+I  KA+                   SA+++L+   
Sbjct: 246 DPMQIILLER--ELFGEVIQILHDQISHKAT------------------RSAMQILVITC 305

Query: 323 ESSKKCRLLLIEAGAVCIMIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIM 382
              +  R   +E G + ++I+LL D    S R   E  + VL +L  CAEGR     H  
Sbjct: 306 PWGRN-RHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGA 365

Query: 383 GIATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSS 442
            IA V+KK +  S   ++  V++L  +  +  +  +L+EM+  G V KL  +L      +
Sbjct: 366 AIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGN 425

Query: 443 STKEKAMKIMKLHGSFWRRFPCFPYEFRDYF 447
            TKEKA +++KLH   WR  PC P    D +
Sbjct: 426 KTKEKAKELLKLHARVWRESPCVPRNLYDSY 433

[Close]

BLAST of Carg10178-RA vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 150.6 bits (379), Expect = 3.0e-36
Identity = 128/437 (29.29%), Postives = 214/437 (48.97%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ-IIVNFDITPN 82
           +IP YF CPIS+E+M DPVT  +G++++R NI KW       +CP T Q + ++ D+TPN
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPN 82

Query: 83  YTLKRLIVSWKM-NESCSSASSSSPRPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 142
           + L+RLI  W + NE+      S+PR P  +  +VV  +KN  L  F   +L +   L  
Sbjct: 83  HMLRRLIQHWCVENETRGVVRISTPRVPPGKL-NVVEEIKN--LKKFGQEALGREETLQK 142

Query: 143 IDDVAAVG-AKPIFVQANGFEVLVGLVTQIAICESSNFANFEACEEALGVLSQFPFSKIE 202
           ++ +A  G  + +  +    + L+  V +    +       +  +E+L +L        +
Sbjct: 143 LEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSND 202

Query: 203 KLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLSKHHAIDLFKSIL 262
               L+     ++S+  VL +     + Y + LL+ +++  Y  + + +    ++FK I+
Sbjct: 203 AKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKGII 262

Query: 263 ELASD---EIIKKASTVKMTDSSIATHRYGTLFSSALE-----------LLIEIMESS-- 322
               D    + + + TV+ T  S +    G    S L+            L+ ++E+S  
Sbjct: 263 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 322

Query: 323 KKCRLLLIEAGAVCIMIDLLPDSNRSK--CEKILHVLKLLSDCAEGRLAMAEHIMGIATV 382
            + R LL++ GAV  +I+L       K   E +L VL  L  CA GR  +  H  GIA V
Sbjct: 323 SRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVV 382

Query: 383 TKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEK 438
           TK+ +  S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D    + KEK
Sbjct: 383 TKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD-CGLNLKEK 442

[Close]

BLAST of Carg10178-RA vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-29
Identity = 126/441 (28.57%), Postives = 205/441 (46.49%), Query Frame = 0


Query: 12  PQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ 71
           P+ + P  +   IP +FRCPIS+E+M DPVT+ TG +++RA+IE W    N  TCP T  
Sbjct: 2   PRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRA 61

Query: 72  IIVNFDITPNYTLKRLIVSWKM-NESCSSASSSSPRPPSMEYDDVVALLKNIELSP---- 131
            + +F + PN+TL+RLI  W + N S       +P+ P+ +   V ALL           
Sbjct: 62  PLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPA-DPTSVRALLSQASAITGTHV 121

Query: 132 ---FKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANFEAC 191
               +A++L++LR      D   V    +    N  E+L+ ++        S   + E  
Sbjct: 122 SVRSRAAALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELV 181

Query: 192 EEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQIS--KKGY 251
            E+L +L   P ++  +   + + P  ++ +  +L   SIE R  A  L++ +S   K  
Sbjct: 182 SESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 241

Query: 252 DYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHRYG--TLFSSALELLIEI 311
           D    S  ++  +F+ +L+L  + I           SS    + G  TLF+         
Sbjct: 242 DLKG-SISNSESVFEGVLDLLRNPI-----------SSRRALKIGIKTLFA--------- 301

Query: 312 MESSKKCRLLLIEAGAVCIMID-LLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 371
           + S K  R + I AGA  I+ID L  D +R   E+ L  ++LL    EG  A  EH + +
Sbjct: 302 LCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTV 361

Query: 372 ATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 431
             + K  +  S+ AT+     L  +C     ER  EE  G G V +L+ ++  +  +   
Sbjct: 362 PLLVKTILRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE-CTERA 406

Query: 432 KEKAMKIMKLHGSFWRRFPCF 439
           K+KA K++KL    W  +  F
Sbjct: 422 KKKAQKLLKLLRDSWPDYNSF 406

[Close]

BLAST of Carg10178-RA vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 5.2e-28
Identity = 110/401 (27.43%), Postives = 191/401 (47.63%), Query Frame = 0


Query: 21  VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITP 80
           +  +P +F+CPIS++VM  PV++STGV+++R +I++W    N  TCPATMQI+ N +  P
Sbjct: 8   ITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVP 67

Query: 81  NYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 140
           N TL RLI  W  +     A S SP   +   D++ A ++   +     S  K LR    
Sbjct: 68  NLTLHRLIDHWS-DSINRRADSESPESDTPTRDEINAAIERFRIENDARS--KILRFARE 127

Query: 141 IDDVAA-VGAKPIFVQANGFEVLVGLVTQIAICESSNFANFE--ACEEALGVLSQ----- 200
            D+    +  K  FV      +LV L++     +S NF++ +     EA+ +LS      
Sbjct: 128 SDENREFLAGKDDFV-----AMLVDLIS-----DSRNFSDSQLLLVGEAVKILSMIRRKI 187

Query: 201 FPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLSKHHAI 260
           F   ++  L  L    + + S  ++++RG+               K   D +++ +  A+
Sbjct: 188 FDRRRLSNLI-LTNGGDCLTSFFLLIKRGN--------------PKLKIDCSAVLEFIAV 247

Query: 261 DLFKSILELASDEIIKKASTVKMTDSSIATHRYGTLFSSALELLIEIMESSKKCRLLLIE 320
           D    ++    + ++ +   +  +DS        +L  + L LLI I  SSK+ +L LI 
Sbjct: 248 DAESKLIIAKGEGLVTEIIKLISSDSD------SSLIEANLSLLIAI-ASSKRVKLALIR 307

Query: 321 AGAVCIMIDLL--PDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMSTSNT 380
              V  +  LL  P ++ S  EK L +L+ +S C EGR  + + +     V K M  S  
Sbjct: 308 EKLVTKLTSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMKVSTA 367

Query: 381 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGD 412
           AT+  V +LW +C     ++  + ++    V K++ +L  +
Sbjct: 368 ATEHAVTVLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

The following BLAST results are available for this feature:

BLAST of Carg10178-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013396.1	4.1e-253	100.00	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6574816.1	8.5e-251	99.56	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022958929.1	1.9e-250	99.11	E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata]	[more]
XP_023549278.1	9.8e-247	97.78	E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo]	[more]
XP_023006772.1	8.3e-238	94.68	E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima]	[more]

BLAST of Carg10178-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	3.5e-45	31.53	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	6.1e-42	29.05	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	4.2e-35	29.29	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	2.3e-28	28.57	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9LXE3	7.3e-27	27.43	U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1...	[more]

BLAST of Carg10178-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6I8	9.2e-251	99.11	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075...	[more]
A0A6J1KWQ8	4.0e-238	94.68	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P...	[more]
A0A0A0KBP6	6.0e-210	85.24	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE...	[more]
A0A1S3CC54	4.5e-205	83.22	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ...	[more]
A0A5D3DLY2	1.8e-182	76.38	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Carg10178-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	2.5e-46	31.53	plant U-box 23	[more]
AT3G52450.1	4.3e-43	29.05	plant U-box 22	[more]
AT3G11840.1	3.0e-36	29.29	plant U-box 24	[more]
AT3G19380.1	1.6e-29	28.57	plant U-box 25	[more]
AT5G09800.1	5.2e-28	27.43	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 27..91 e-value: 9.2E-22 score: 88.3
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 24..95 e-value: 2.9E-16 score: 59.4
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 23..98 score: 29.334106
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 20..103 e-value: 4.3E-23 score: 83.1
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 115..431 e-value: 6.8E-12 score: 46.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 24..448
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 24..448
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 28..71 e-value: 3.39201E-21 score: 84.2074
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 20..94
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 179..425

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg10178	Carg10178	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg10178-RA:3	Carg10178-RA:3	exon
Carg10178-RA:2	Carg10178-RA:2	exon
Carg10178-RA:1	Carg10178-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg10178-RA:cds	Carg10178-RA:cds_3	CDS
Carg10178-RA:cds	Carg10178-RA:cds_2	CDS
Carg10178-RA:cds	Carg10178-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg10178-RA	Carg10178-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity