Carg08665-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08665-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ABC transporter B family member 13-like
Location	Carg_Chr09: 420477 .. 422086 (-)
Sequence length	855
RNA-Seq Expression	Carg08665-RA
Synteny	Carg08665-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAATCTCGCTGCCATCGCAAAAGGTCGGGTGGCTGCAGCCAACATCTTTAGCATGATTGACTCAGGTTATGAATCGTGGTCGAGGTCGGATAATGAAGTAGCATTGTCAAATGTAGCAGGGAAGATTGAGTTTTCTGAGGTTTCCTTTGCTTATCCCTCCCGACCCCAACTGATTTTTGAGGAGTTAAGCTTCTCAATAAGTGCTGGAAAGACAGTTGCAGTTGTTGGCCCCAGTGGCTCAGGAAAGAGTACCATTGTTTCCATGGTTCAGCGTTTCTACGAACCATCTTCAGGTTGGTTGAGTCATGAAATATCCTTGGCAGTTCTATGGTCTCGTGTCGGCTCGCCCACTGATTATTTGAAGTTTATTAGGTAAGATACTATTGGATGGATATGATCTTAGGTCTCTTGATTTGAAATGGTTGAGAAGGCAAATGGGGTTGGTTAGTCAAGAGCCTGCCTTGTTTAGTACCACAATAGCTGCCAATATTCTTTTTGGTCAAGAACATGCAGCCATGGATGAGATCATTGCAGCTGCTCAAGCTGCTAATGCTCATTCCTTCATTCAAGAACTACCAGATGGTTACTCAACTCCGGTTCGTGTCCTCTAATCTTAATACTCGTTTGAAATTCATTTAATTTGAGAACGACTTACTTCCGGGAGTCGACGTGTAGGTCGGAGAGGGAGGGACGCAGCTTTCAGGTGGGCAAAAGCAGAGGATTGCCCTTGCAAGGGCAGTTTTACGAAATCCAAAAATATTACTTCTAGATGAGGCAACCAGTGCTCTTGATGCAGAATCAGAACTCATTGTTCAGCAGGCACTGGACAGAATCATGTCGAATCGAACGACGATCATCGTTGCACATCGACTTTCCACAATTCAAGATGCCGATACCATCACTGTCCTGAAAAACGGTCAAATTGTTGAGAGTGGAAATCATTCAGAATTGATGTCAAACAATGGTGAGTATGCAGCTCTAGTAAGCTTGCAAGTATCAGACCAAGTGAATGATTGTAGCATAATATCTCCATCTAGGAGTTCCGGGCGTTCTAGTTTTCGAGAATCTTTTAGCTTTCATAACAGTATACAAGATTCCAAGTCATTCAGAGAGACTGAGCTACAATCAGCCAACAAAGATTCAAAAACTTCGAATTCTCCTCCGTCGATATGGGAGCTACTGAAGTTAAATGCACCCGAGTGGCGTTATGCAGTACTAGGATCGATCGGGGCAATTCTTGCAGGCATTCAAGCTCCTTTGTTTGCACTTGGCATAACCCATGTCCTATCTGCATTTTATTCTCCTCATCATTCTCAAATCAAAGAAGAGGTCCAACATGTCGCCTATGTGTTTGTTGGAGTTGCTATTCTCACCATTCCTGTATATCTGTTGCAACACTACTTCTATACCTTAATGGGAGAGAGACTTACCGCTCGTGTTCGTTTGCTGTTATTCTCAGGTTTCTTCGTGTTCTTAAACGATATCGCTCGGGATTTTATAGTCACCGATGTTTACAAATTGATTCTTTTTCATCCATTTTGTCTGATGTTTGCCATCAACCAATTGCAGCTATCCTTTCCAACGAAGTTGGTTGGTTTGATTTAG

mRNA sequence

ATGCCGAATCTCGCTGCCATCGCAAAAGGTCGGGTGGCTGCAGCCAACATCTTTAGCATGATTGACTCAGGTTATGAATCGTGGTCGAGGTCGGATAATGAAGTAGCATTGTCAAATGTAGCAGGGAAGATTGAGTTTTCTGAGGTTTCCTTTGCTTATCCCTCCCGACCCCAACTGATTTTTGAGGAGTTAAGCTTCTCAATAAGTGCTGGAAAGACAGTTGCAGTTGTTGGCCCCAGTGGCTCAGGAAAGAGTACCATTGTTTCCATGGTTCAGCGTTTCTACGAACCATCTTCAGGTTGGTTGAGTCATGAAATATCCTTGGCAGTTCTATGTATACAAGATTCCAAGTCATTCAGAGAGACTGAGCTACAATCAGCCAACAAAGATTCAAAAACTTCGAATTCTCCTCCGTCGATATGGGAGCTACTGAAGTTAAATGCACCCGAGTGGCGTTATGCAGTACTAGGATCGATCGGGGCAATTCTTGCAGGCATTCAAGCTCCTTTGTTTGCACTTGGCATAACCCATGTCCTATCTGCATTTTATTCTCCTCATCATTCTCAAATCAAAGAAGAGGTCCAACATGTCGCCTATGTGTTTGTTGGAGTTGCTATTCTCACCATTCCTGTATATCTGTTGCAACACTACTTCTATACCTTAATGGGAGAGAGACTTACCGCTCGTGTTCGTTTGCTGTTATTCTCAGGTTTCTTCGTGTTCTTAAACGATATCGCTCGGGATTTTATAGTCACCGATGTTTACAAATTGATTCTTTTTCATCCATTTTGTCTGATGTTTGCCATCAACCAATTGCAGCTATCCTTTCCAACGAAGTTGGTTGGTTTGATTTAG

Coding sequence (CDS)

ATGCCGAATCTCGCTGCCATCGCAAAAGGTCGGGTGGCTGCAGCCAACATCTTTAGCATGATTGACTCAGGTTATGAATCGTGGTCGAGGTCGGATAATGAAGTAGCATTGTCAAATGTAGCAGGGAAGATTGAGTTTTCTGAGGTTTCCTTTGCTTATCCCTCCCGACCCCAACTGATTTTTGAGGAGTTAAGCTTCTCAATAAGTGCTGGAAAGACAGTTGCAGTTGTTGGCCCCAGTGGCTCAGGAAAGAGTACCATTGTTTCCATGGTTCAGCGTTTCTACGAACCATCTTCAGGTTGGTTGAGTCATGAAATATCCTTGGCAGTTCTATGTATACAAGATTCCAAGTCATTCAGAGAGACTGAGCTACAATCAGCCAACAAAGATTCAAAAACTTCGAATTCTCCTCCGTCGATATGGGAGCTACTGAAGTTAAATGCACCCGAGTGGCGTTATGCAGTACTAGGATCGATCGGGGCAATTCTTGCAGGCATTCAAGCTCCTTTGTTTGCACTTGGCATAACCCATGTCCTATCTGCATTTTATTCTCCTCATCATTCTCAAATCAAAGAAGAGGTCCAACATGTCGCCTATGTGTTTGTTGGAGTTGCTATTCTCACCATTCCTGTATATCTGTTGCAACACTACTTCTATACCTTAATGGGAGAGAGACTTACCGCTCGTGTTCGTTTGCTGTTATTCTCAGGTTTCTTCGTGTTCTTAAACGATATCGCTCGGGATTTTATAGTCACCGATGTTTACAAATTGATTCTTTTTCATCCATTTTGTCTGATGTTTGCCATCAACCAATTGCAGCTATCCTTTCCAACGAAGTTGGTTGGTTTGATTTAG

Protein sequence

MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLIFEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLSHEISLAVLCIQDSKSFRETELQSANKDSKTSNSPPSIWELLKLNAPEWRYAVLGSIGAILAGIQAPLFALGITHVLSAFYSPHHSQIKEEVQHVAYVFVGVAILTIPVYLLQHYFYTLMGERLTARVRLLLFSGFFVFLNDIARDFIVTDVYKLILFHPFCLMFAINQLQLSFPTKLVGLI

Homology

[Close]

BLAST of Carg08665-RA vs. NCBI nr
Match: KAG7024142.1 (ABC transporter B family member 14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 545.4 bits (1404), Expect = 2.8e-151
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI
Sbjct: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLSHEISLAVLCIQDSKSFR 120
           FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLSHEISLAVLCIQDSKSFR
Sbjct: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLSHEISLAVLCIQDSKSFR 120

Query: 121 ETELQSANKDSKTSNSPPSIWELLKLNAPEWRYAVLGSIGAILAGIQAPLFALGITHVLS 180
           ETELQSANKDSKTSNSPPSIWELLKLNAPEWRYAVLGSIGAILAGIQAPLFALGITHVLS
Sbjct: 121 ETELQSANKDSKTSNSPPSIWELLKLNAPEWRYAVLGSIGAILAGIQAPLFALGITHVLS 180

Query: 181 AFYSPHHSQIKEEVQHVAYVFVGVAILTIPVYLLQHYFYTLMGERLTARVRLLLFSGFFV 240
           AFYSPHHSQIKEEVQHVAYVFVGVAILTIPVYLLQHYFYTLMGERLTARVRLLLFSGFFV
Sbjct: 181 AFYSPHHSQIKEEVQHVAYVFVGVAILTIPVYLLQHYFYTLMGERLTARVRLLLFSGFFV 240

Query: 241 FLNDIARDFIVTDVYKLILFHPFCLMFAINQLQLSFPTKLVGLI 285
           FLNDIARDFIVTDVYKLILFHPFCLMFAINQLQLSFPTKLVGLI
Sbjct: 241 FLNDIARDFIVTDVYKLILFHPFCLMFAINQLQLSFPTKLVGLI 284

[Close]

BLAST of Carg08665-RA vs. NCBI nr
Match: KAG6591261.1 (ABC transporter B family member 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 367.9 bits (943), Expect = 8.1e-98
Identity = 235/437 (53.78%), Postives = 235/437 (53.78%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI
Sbjct: 328 MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 387

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLSHEISLAVL--------- 120
           FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLSHEISLAVL         
Sbjct: 388 FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLSHEISLAVLWSLDLKWLR 447

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 448 RQMGLVSQEPALFSTTIAANILFGQEHAAMDEIIAAAQAANAHSFIQELPDGYSTPVGEG 507

Query: 181 ------------------------------------------------------------ 237
                                                                       
Sbjct: 508 GTQLSGGQKQRIALARAVLRNPKILLLDEATSALDAESELIVQQALDRIMSNRTTIIVAH 567

[Close]

BLAST of Carg08665-RA vs. NCBI nr
Match: XP_022936707.1 (ABC transporter B family member 13-like [Cucurbita moschata])

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-92
Identity = 228/435 (52.41%), Postives = 232/435 (53.33%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSN+AGKIEFSEVSFAYPSRPQLI
Sbjct: 328 MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNIAGKIEFSEVSFAYPSRPQLI 387

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 388 FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGYDLRSLDLKWLRRQ 447

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 448 MGLVSQEPALFSTTIAANILFGQEHAAMDEIIAAAQAANAHSFIQELPDGYSTPVGEGGT 507

Query: 181 --------------AVL------------------------------------------- 237
                         AVL                                           
Sbjct: 508 QLSGGQKQRIALARAVLRNPKILLLDEATSALDAESELIVQQALDRIMSNRTTIIVAHRL 567

[Close]

BLAST of Carg08665-RA vs. NCBI nr
Match: XP_023535471.1 (ABC transporter B family member 13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 346.7 bits (888), Expect = 1.9e-91
Identity = 226/435 (51.95%), Postives = 231/435 (53.10%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI
Sbjct: 328 MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 387

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 388 FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGYDLRSLDLKWLRRQ 447

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 448 MGLVSQEPALFSTTIAANILFGQEHAAMDEIIAAAQAANAHSFIQELPDGYSTSVGEGGT 507

Query: 181 --------------AVL------------------------------------------- 237
                         AVL                                           
Sbjct: 508 QLSGGQKQRIALARAVLRNPKILLLDEATSALDAESELIVQQALDRIMSNRTTIIVAHRL 567

[Close]

BLAST of Carg08665-RA vs. NCBI nr
Match: XP_022975816.1 (ABC transporter B family member 13-like [Cucurbita maxima])

HSP 1 Score: 339.3 bits (869), Expect = 3.1e-89
Identity = 225/435 (51.72%), Postives = 229/435 (52.64%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMIDSGYES SR DNEV+LSNVAGKIEFSEVSFAYPSRPQLI
Sbjct: 328 MPNLAAIAKGRVAAANIFSMIDSGYESSSRLDNEVSLSNVAGKIEFSEVSFAYPSRPQLI 387

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 388 FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGYDLRSLDLKWLRRQ 447

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 448 MGLVSQEPALFSTTIAANILFGQEHAAMDEIIAAAQAANAHSFIQELPDGYSTLVGEGGT 507

Query: 181 --------------AVL------------------------------------------- 237
                         AVL                                           
Sbjct: 508 QLSGGQKQRIALARAVLRNPKILLLDEATSALDAESELMVQQALDRIMSNRTTIIVAHRL 567

[Close]

BLAST of Carg08665-RA vs. ExPASy Swiss-Prot
Match: Q9C7F8 (ABC transporter B family member 13 OS=Arabidopsis thaliana OX=3702 GN=ABCB13 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 6.6e-50
Identity = 149/435 (34.25%), Postives = 186/435 (42.76%), Query Frame = 0


Query: 2   PNLAAIAKGRVAAANIFSMI-DSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 61
           P+L+AIAKGRVAAANIF MI ++  ES  R D    L NVAG+IEF +VSFAYPSRP ++
Sbjct: 329 PSLSAIAKGRVAAANIFRMIGNNNSESSQRLDEGTTLQNVAGRIEFQKVSFAYPSRPNMV 388

Query: 62  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 121
           FE LSF+I +GKT A VGPSGSGKSTI+SMVQRFYEP+SG               W   +
Sbjct: 389 FENLSFTIRSGKTFAFVGPSGSGKSTIISMVQRFYEPNSGEILLDGNDIKSLKLKWFREQ 448

Query: 122 ISLA-------------------------------------------------------- 181
           + L                                                         
Sbjct: 449 LGLVSQEPALFATTIASNILLGKENANMDQIIEAAKAANADSFIKSLPNGYNTQVGEGGT 508

Query: 182 ------------------------------------------------------------ 237
                                                                       
Sbjct: 509 QLSGGQKQRIAIARAVLRNPKILLLDEATSALDAESEKIVQQALDNVMEKRTTIVVAHRL 568

[Close]

BLAST of Carg08665-RA vs. ExPASy Swiss-Prot
Match: Q9C7F2 (ABC transporter B family member 14 OS=Arabidopsis thaliana OX=3702 GN=ABCB14 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.6e-48
Identity = 149/438 (34.02%), Postives = 184/438 (42.01%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMI-DSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL 60
           +P+L+AI+KGRVAAANIF MI ++  ES  R +N   L NV GKIEF  VSFAYPSRP +
Sbjct: 329 VPSLSAISKGRVAAANIFKMIGNNNLESSERLENGTTLQNVVGKIEFCGVSFAYPSRPNM 388

Query: 61  IFEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSH 120
           +FE LSF+I +GKT A VGPSGSGKSTI+SMVQRFYEP SG               WL  
Sbjct: 389 VFENLSFTIHSGKTFAFVGPSGSGKSTIISMVQRFYEPRSGEILLDGNDIKNLKLKWLRE 448

Query: 121 EISLA------------------------------------------------------- 180
           ++ L                                                        
Sbjct: 449 QMGLVSQEPALFATTIASNILLGKEKANMDQIIEAAKAANADSFIKSLPNGYNTQVGEGG 508

Query: 181 ------------------------------------------------------------ 237
                                                                       
Sbjct: 509 TQLSGGQKQRIAIARAVLRNPKILLLDEATSALDAESEKIVQQALDNVMEKRTTIVIAHR 568

[Close]

BLAST of Carg08665-RA vs. ExPASy Swiss-Prot
Match: Q9ZR72 (ABC transporter B family member 1 OS=Arabidopsis thaliana OX=3702 GN=ABCB1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.1e-19
Identity = 50/100 (50.00%), Postives = 69/100 (69.00%), Query Frame = 0


Query: 2   PNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL-I 61
           P++AA AK +VAAA IF +ID        S++ V L +V G +E   V F+YPSRP + I
Sbjct: 326 PSMAAFAKAKVAAAKIFRIIDHKPTIERNSESGVELDSVTGLVELKNVDFSYPSRPDVKI 385

Query: 62  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG 101
                 S+ AGKT+A+VG SGSGKST+VS+++RFY+P+SG
Sbjct: 386 LNNFCLSVPAGKTIALVGSSGSGKSTVVSLIERFYDPNSG 425

HSP 2 Score: 83.2 bits (204), Expect = 5.3e-15
Identity = 40/108 (37.04%), Postives = 68/108 (62.96%), Query Frame = 0


Query: 139 SIWELLKLNAPEWRYAVLGSIGAILAGIQAPLFALGITHVLSAFYSPHHSQIKEEVQHVA 198
           S W L K+N+PEW+YA+LGS+G+++ G  +  FA  ++ VLS +Y+P H  + +++    
Sbjct: 685 SFWRLAKMNSPEWKYALLGSVGSVICGSLSAFFAYVLSAVLSVYYNPDHEYMIKQIDKYC 744

Query: 199 YVFVGVAILTIPVYLLQHYFYTLMGERLTARVRLLLFSGFFVFLNDIA 247
           Y+ +G++   +    LQH F+ ++GE LT RVR  + S   V  N++A
Sbjct: 745 YLLIGLSSAALVFNTLQHSFWDIVGENLTKRVREKMLSA--VLKNEMA 790

[Close]

BLAST of Carg08665-RA vs. ExPASy Swiss-Prot
Match: Q9FWX7 (ABC transporter B family member 11 OS=Arabidopsis thaliana OX=3702 GN=ABCB11 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.0e-18
Identity = 49/97 (50.52%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 8    AKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL-IFEELSF 67
            +K +VAAA+IF++ID   +  S  +    L NV G IE   +SF YP+RP + IF +L  
Sbjct: 996  SKAKVAAASIFAIIDRKSKIDSSDETGTVLENVKGDIELRHLSFTYPARPDIQIFRDLCL 1055

Query: 68   SISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLS 104
            +I AGKTVA+VG SGSGKST++S++QRFY+P SG ++
Sbjct: 1056 TIRAGKTVALVGESGSGKSTVISLLQRFYDPDSGHIT 1092

[Close]

BLAST of Carg08665-RA vs. ExPASy Swiss-Prot
Match: Q9M0M2 (ABC transporter B family member 9 OS=Arabidopsis thaliana OX=3702 GN=ABCB9 PE=3 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 1.3e-18
Identity = 50/93 (53.76%), Postives = 65/93 (69.89%), Query Frame = 0


Query: 9    KGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL-IFEELSFS 68
            K + +AA+IF ++DS  +  S SD    L NV G IEF  VSF YP RP + IF +L  +
Sbjct: 958  KAKDSAASIFDILDSTPKIDSSSDEGTTLQNVNGDIEFRHVSFRYPMRPDVQIFRDLCLT 1017

Query: 69   ISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG 101
            I +GKTVA+VG SGSGKST++SM++RFY P SG
Sbjct: 1018 IPSGKTVALVGESGSGKSTVISMIERFYNPDSG 1050

[Close]

BLAST of Carg08665-RA vs. ExPASy TrEMBL
Match: A0A6J1F967 (ABC transporter B family member 13-like OS=Cucurbita moschata OX=3662 GN=LOC111443220 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 6.5e-93
Identity = 228/435 (52.41%), Postives = 232/435 (53.33%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSN+AGKIEFSEVSFAYPSRPQLI
Sbjct: 328 MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNIAGKIEFSEVSFAYPSRPQLI 387

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 388 FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGYDLRSLDLKWLRRQ 447

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 448 MGLVSQEPALFSTTIAANILFGQEHAAMDEIIAAAQAANAHSFIQELPDGYSTPVGEGGT 507

Query: 181 --------------AVL------------------------------------------- 237
                         AVL                                           
Sbjct: 508 QLSGGQKQRIALARAVLRNPKILLLDEATSALDAESELIVQQALDRIMSNRTTIIVAHRL 567

[Close]

BLAST of Carg08665-RA vs. ExPASy TrEMBL
Match: A0A6J1IF93 (ABC transporter B family member 13-like OS=Cucurbita maxima OX=3661 GN=LOC111476409 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.5e-89
Identity = 225/435 (51.72%), Postives = 229/435 (52.64%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMIDSGYES SR DNEV+LSNVAGKIEFSEVSFAYPSRPQLI
Sbjct: 328 MPNLAAIAKGRVAAANIFSMIDSGYESSSRLDNEVSLSNVAGKIEFSEVSFAYPSRPQLI 387

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 388 FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGYDLRSLDLKWLRRQ 447

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 448 MGLVSQEPALFSTTIAANILFGQEHAAMDEIIAAAQAANAHSFIQELPDGYSTLVGEGGT 507

Query: 181 --------------AVL------------------------------------------- 237
                         AVL                                           
Sbjct: 508 QLSGGQKQRIALARAVLRNPKILLLDEATSALDAESELMVQQALDRIMSNRTTIIVAHRL 567

[Close]

BLAST of Carg08665-RA vs. ExPASy TrEMBL
Match: A0A0A0LH66 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873270 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 3.1e-79
Identity = 209/436 (47.94%), Postives = 222/436 (50.92%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIFSMID+ YES SRS+N VALS+VAGKIEFSEVSFAYPSRPQLI
Sbjct: 325 MPNLAAIAKGRVAAANIFSMIDADYESSSRSNNGVALSSVAGKIEFSEVSFAYPSRPQLI 384

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           F++LSFSISAG+TVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 385 FDKLSFSISAGRTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGHDLRTLELKWLRRQ 444

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 445 MGLVSQEPALFNTTIAANILFGQENATMDEIIAAAEVANAHSFIQELPDGYSTQVGERGI 504

Query: 181 --------------AVL------------------------------------------- 237
                         AVL                                           
Sbjct: 505 QLSGGQKQRIAIARAVLRNPKILLLDEATSALDSESELIVQQALVRIMLNRTTIIIAHRL 564

[Close]

BLAST of Carg08665-RA vs. ExPASy TrEMBL
Match: A0A6J1CHA7 (ABC transporter B family member 13-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011634 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.2e-73
Identity = 202/447 (45.19%), Postives = 223/447 (49.89%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIF+MID    S++ S+N V LSNVAG+IEFSEVSFAYPSRPQLI
Sbjct: 114 MPNLAAIAKGRVAAANIFNMIDKDSNSYTSSNNGVTLSNVAGRIEFSEVSFAYPSRPQLI 173

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           F++LSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 174 FDKLSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGHDLRSLELKWLRRQ 233

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 234 MGLVSQEPALFATTIASNILLGQENAAMDEIIEAAKSANAHSFIQELPDGYSTQVGEGGT 293

Query: 181 --------------AVL------------------------------------------- 240
                         AVL                                           
Sbjct: 294 QLSGGQKQRIAIARAVLRNPKILLLDEATSALDAESELIVQQALDRIMSNRTTIIIAHRL 353

Query: 241 -CIQD------------------------------------------------------- 247
             IQD                                                       
Sbjct: 354 STIQDVDTIIVLKNGQVVESGNHSELMSKSGEYAALVKLQVSDQVNDSSIESPSGSSRYS 413

[Close]

BLAST of Carg08665-RA vs. ExPASy TrEMBL
Match: A0A6J1CH92 (ABC transporter B family member 13-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011634 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.2e-73
Identity = 202/447 (45.19%), Postives = 223/447 (49.89%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 60
           MPNLAAIAKGRVAAANIF+MID    S++ S+N V LSNVAG+IEFSEVSFAYPSRPQLI
Sbjct: 328 MPNLAAIAKGRVAAANIFNMIDKDSNSYTSSNNGVTLSNVAGRIEFSEVSFAYPSRPQLI 387

Query: 61  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 120
           F++LSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG               WL  +
Sbjct: 388 FDKLSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGKILLDGHDLRSLELKWLRRQ 447

Query: 121 ISL--------------------------------------------------------- 180
           + L                                                         
Sbjct: 448 MGLVSQEPALFATTIASNILLGQENAAMDEIIEAAKSANAHSFIQELPDGYSTQVGEGGT 507

Query: 181 --------------AVL------------------------------------------- 240
                         AVL                                           
Sbjct: 508 QLSGGQKQRIAIARAVLRNPKILLLDEATSALDAESELIVQQALDRIMSNRTTIIIAHRL 567

Query: 241 -CIQD------------------------------------------------------- 247
             IQD                                                       
Sbjct: 568 STIQDVDTIIVLKNGQVVESGNHSELMSKSGEYAALVKLQVSDQVNDSSIESPSGSSRYS 627

[Close]

BLAST of Carg08665-RA vs. TAIR 10
Match: AT1G27940.1 (P-glycoprotein 13 )

HSP 1 Score: 199.1 bits (505), Expect = 4.7e-51
Identity = 149/435 (34.25%), Postives = 186/435 (42.76%), Query Frame = 0


Query: 2   PNLAAIAKGRVAAANIFSMI-DSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQLI 61
           P+L+AIAKGRVAAANIF MI ++  ES  R D    L NVAG+IEF +VSFAYPSRP ++
Sbjct: 329 PSLSAIAKGRVAAANIFRMIGNNNSESSQRLDEGTTLQNVAGRIEFQKVSFAYPSRPNMV 388

Query: 62  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSHE 121
           FE LSF+I +GKT A VGPSGSGKSTI+SMVQRFYEP+SG               W   +
Sbjct: 389 FENLSFTIRSGKTFAFVGPSGSGKSTIISMVQRFYEPNSGEILLDGNDIKSLKLKWFREQ 448

Query: 122 ISLA-------------------------------------------------------- 181
           + L                                                         
Sbjct: 449 LGLVSQEPALFATTIASNILLGKENANMDQIIEAAKAANADSFIKSLPNGYNTQVGEGGT 508

Query: 182 ------------------------------------------------------------ 237
                                                                       
Sbjct: 509 QLSGGQKQRIAIARAVLRNPKILLLDEATSALDAESEKIVQQALDNVMEKRTTIVVAHRL 568

[Close]

BLAST of Carg08665-RA vs. TAIR 10
Match: AT1G28010.1 (P-glycoprotein 14 )

HSP 1 Score: 193.4 bits (490), Expect = 2.6e-49
Identity = 149/438 (34.02%), Postives = 184/438 (42.01%), Query Frame = 0


Query: 1   MPNLAAIAKGRVAAANIFSMI-DSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL 60
           +P+L+AI+KGRVAAANIF MI ++  ES  R +N   L NV GKIEF  VSFAYPSRP +
Sbjct: 329 VPSLSAISKGRVAAANIFKMIGNNNLESSERLENGTTLQNVVGKIEFCGVSFAYPSRPNM 388

Query: 61  IFEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG---------------WLSH 120
           +FE LSF+I +GKT A VGPSGSGKSTI+SMVQRFYEP SG               WL  
Sbjct: 389 VFENLSFTIHSGKTFAFVGPSGSGKSTIISMVQRFYEPRSGEILLDGNDIKNLKLKWLRE 448

Query: 121 EISLA------------------------------------------------------- 180
           ++ L                                                        
Sbjct: 449 QMGLVSQEPALFATTIASNILLGKEKANMDQIIEAAKAANADSFIKSLPNGYNTQVGEGG 508

Query: 181 ------------------------------------------------------------ 237
                                                                       
Sbjct: 509 TQLSGGQKQRIAIARAVLRNPKILLLDEATSALDAESEKIVQQALDNVMEKRTTIVIAHR 568

[Close]

BLAST of Carg08665-RA vs. TAIR 10
Match: AT2G36910.1 (ATP binding cassette subfamily B1 )

HSP 1 Score: 97.8 bits (242), Expect = 1.5e-20
Identity = 50/100 (50.00%), Postives = 69/100 (69.00%), Query Frame = 0


Query: 2   PNLAAIAKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL-I 61
           P++AA AK +VAAA IF +ID        S++ V L +V G +E   V F+YPSRP + I
Sbjct: 326 PSMAAFAKAKVAAAKIFRIIDHKPTIERNSESGVELDSVTGLVELKNVDFSYPSRPDVKI 385

Query: 62  FEELSFSISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG 101
                 S+ AGKT+A+VG SGSGKST+VS+++RFY+P+SG
Sbjct: 386 LNNFCLSVPAGKTIALVGSSGSGKSTVVSLIERFYDPNSG 425

HSP 2 Score: 83.2 bits (204), Expect = 3.7e-16
Identity = 40/108 (37.04%), Postives = 68/108 (62.96%), Query Frame = 0


Query: 139 SIWELLKLNAPEWRYAVLGSIGAILAGIQAPLFALGITHVLSAFYSPHHSQIKEEVQHVA 198
           S W L K+N+PEW+YA+LGS+G+++ G  +  FA  ++ VLS +Y+P H  + +++    
Sbjct: 685 SFWRLAKMNSPEWKYALLGSVGSVICGSLSAFFAYVLSAVLSVYYNPDHEYMIKQIDKYC 744

Query: 199 YVFVGVAILTIPVYLLQHYFYTLMGERLTARVRLLLFSGFFVFLNDIA 247
           Y+ +G++   +    LQH F+ ++GE LT RVR  + S   V  N++A
Sbjct: 745 YLLIGLSSAALVFNTLQHSFWDIVGENLTKRVREKMLSA--VLKNEMA 790

[Close]

BLAST of Carg08665-RA vs. TAIR 10
Match: AT1G02520.1 (P-glycoprotein 11 )

HSP 1 Score: 95.5 bits (236), Expect = 7.3e-20
Identity = 49/97 (50.52%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 8    AKGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL-IFEELSF 67
            +K +VAAA+IF++ID   +  S  +    L NV G IE   +SF YP+RP + IF +L  
Sbjct: 996  SKAKVAAASIFAIIDRKSKIDSSDETGTVLENVKGDIELRHLSFTYPARPDIQIFRDLCL 1055

Query: 68   SISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSGWLS 104
            +I AGKTVA+VG SGSGKST++S++QRFY+P SG ++
Sbjct: 1056 TIRAGKTVALVGESGSGKSTVISLLQRFYDPDSGHIT 1092

[Close]

BLAST of Carg08665-RA vs. TAIR 10
Match: AT4G18050.1 (P-glycoprotein 9 )

HSP 1 Score: 95.1 bits (235), Expect = 9.5e-20
Identity = 50/93 (53.76%), Postives = 65/93 (69.89%), Query Frame = 0


Query: 9    KGRVAAANIFSMIDSGYESWSRSDNEVALSNVAGKIEFSEVSFAYPSRPQL-IFEELSFS 68
            K + +AA+IF ++DS  +  S SD    L NV G IEF  VSF YP RP + IF +L  +
Sbjct: 958  KAKDSAASIFDILDSTPKIDSSSDEGTTLQNVNGDIEFRHVSFRYPMRPDVQIFRDLCLT 1017

Query: 69   ISAGKTVAVVGPSGSGKSTIVSMVQRFYEPSSG 101
            I +GKTVA+VG SGSGKST++SM++RFY P SG
Sbjct: 1018 IPSGKTVALVGESGSGKSTVISMIERFYNPDSG 1050

The following BLAST results are available for this feature:

BLAST of Carg08665-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024142.1	2.8e-151	100.00	ABC transporter B family member 14, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6591261.1	8.1e-98	53.78	ABC transporter B family member 13, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022936707.1	1.3e-92	52.41	ABC transporter B family member 13-like [Cucurbita moschata]	[more]
XP_023535471.1	1.9e-91	51.95	ABC transporter B family member 13-like [Cucurbita pepo subsp. pepo]	[more]
XP_022975816.1	3.1e-89	51.72	ABC transporter B family member 13-like [Cucurbita maxima]	[more]

BLAST of Carg08665-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7F8	6.6e-50	34.25	ABC transporter B family member 13 OS=Arabidopsis thaliana OX=3702 GN=ABCB13 PE=...	[more]
Q9C7F2	3.6e-48	34.02	ABC transporter B family member 14 OS=Arabidopsis thaliana OX=3702 GN=ABCB14 PE=...	[more]
Q9ZR72	2.1e-19	50.00	ABC transporter B family member 1 OS=Arabidopsis thaliana OX=3702 GN=ABCB1 PE=1 ...	[more]
Q9FWX7	1.0e-18	50.52	ABC transporter B family member 11 OS=Arabidopsis thaliana OX=3702 GN=ABCB11 PE=...	[more]
Q9M0M2	1.3e-18	53.76	ABC transporter B family member 9 OS=Arabidopsis thaliana OX=3702 GN=ABCB9 PE=3 ...	[more]

BLAST of Carg08665-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F967	6.5e-93	52.41	ABC transporter B family member 13-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1IF93	1.5e-89	51.72	ABC transporter B family member 13-like OS=Cucurbita maxima OX=3661 GN=LOC111476...	[more]
A0A0A0LH66	3.1e-79	47.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873270 PE=4 SV=1	[more]
A0A6J1CHA7	1.2e-73	45.19	ABC transporter B family member 13-like isoform X3 OS=Momordica charantia OX=367...	[more]
A0A6J1CH92	1.2e-73	45.19	ABC transporter B family member 13-like isoform X1 OS=Momordica charantia OX=367...	[more]

BLAST of Carg08665-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G27940.1	4.7e-51	34.25	P-glycoprotein 13	[more]
AT1G28010.1	2.6e-49	34.02	P-glycoprotein 14	[more]
AT2G36910.1	1.5e-20	50.00	ATP binding cassette subfamily B1	[more]
AT1G02520.1	7.3e-20	50.52	P-glycoprotein 11	[more]
AT4G18050.1	9.5e-20	53.76	P-glycoprotein 9	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003439	ABC transporter-like, ATP-binding domain	PFAM	PF00005	ABC_tran	coord: 62..112 e-value: 6.5E-10 score: 39.7
IPR036640	ABC transporter type 1, transmembrane domain superfamily	GENE3D	1.20.1560.10	ABC transporter type 1, transmembrane domain	coord: 1..41 e-value: 3.9E-33 score: 116.5 coord: 112..239 e-value: 6.9E-19 score: 69.5
IPR036640	ABC transporter type 1, transmembrane domain superfamily	SUPERFAMILY	90123	ABC transporter transmembrane region	coord: 142..236
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 42..100 e-value: 3.9E-33 score: 116.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 33..119
IPR039421	Type 1 protein exporter	PANTHER	PTHR24221	ATP-BINDING CASSETTE SUB-FAMILY B	coord: 2..101 coord: 127..237
None	No IPR available	PANTHER	PTHR24221:SF541	BNAA09G27580D PROTEIN	coord: 2..101 coord: 127..237

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg08665	Carg08665	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08665-RA:1	Carg08665-RA:1	exon
Carg08665-RA:2	Carg08665-RA:2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08665-RA:cds	Carg08665-RA:cds_2	CDS
Carg08665-RA:cds	Carg08665-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg08665-RA	Carg08665-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding