Carg08566-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08566-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionethylene-responsive transcription factor ERF061-like
LocationCarg_Chr02: 4931721 .. 4932623 (-)
Sequence length903
RNA-Seq ExpressionCarg08566-RA
SyntenyCarg08566-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAACCATGACGAACGGAGGAACTCTTCAATTCACGGATGTTTCAACGGCTCTTTCCAATCTCATTCTAACTGCCGGAGGAAATACTCTGGATTCAATCTTCTCTCACTGCGACAATTTCAGCTGCTCTGTAATCGACGGCGTAGATTTCGACCCGGCAGTTGGGTCGTCGGTGTATCTCCGACAGCGGGAAGTACTGCAGAGATTTTCTCAGGAGAGGAAAGCAAATGGGTCTCGGGATTTGTTTGCAAAGGCGTACGAATTGCTGTATTCGGGTTGCGGCGCTGCCGTGAGTGGCGGCAAGAGGAAGGTTTACAGAGGAGTGCGGCAGAGACAATGGGGGAAATGGGTGGCGGAGATTCGACTGCCGCAGAATCGGATGAGGGTGTGGTTAGGAACCTATGACTCGCCGGAGACCGCCGCCTATGCTTACGACTGCGCCGCCTGCAAACTCCGAGGGGAATATGCTCGGTTGAATTTTCCAAATTTGAAGGATCTAAAGAGCGATTTAGGTACCGACGAGTTTGTGAAATTGAGTGGGTTGAAAAAATTGGTGGATGCCAAAATTCAAGCGATTTTTCAGAAGATAAAGGGGAAGGGGAAGAAGAAGGATTGTCGGAGAATCGGCGGCGATTTAGGATCGGGTTCGTGTTCTTCGTCATCATCGTCGGTGTCTCTGCCTCCGGCGGTAGTGGAGATGACGGAAGAATGGAGTTGGGAAAGGGTATCGCCTGTTGGTGCAGCGGAAGATGGGTTGTGGAACTTTGAGAATTCGCCTCGAGCTGGTTCAGTGGATTGCTTGACGGCGACGGCGGCGGCGGGATCGGAAACAGAGTGGTACTCGTTGGAGAAGATGCCTTCTTTTGATGCAGAACAGATTTGGGATGTTCTTGCAAACTAG

mRNA sequence

ATGGAAACCATGACGAACGGAGGAACTCTTCAATTCACGGATGTTTCAACGGCTCTTTCCAATCTCATTCTAACTGCCGGAGGAAATACTCTGGATTCAATCTTCTCTCACTGCGACAATTTCAGCTGCTCTGTAATCGACGGCGTAGATTTCGACCCGGCAGTTGGGTCGTCGGTGTATCTCCGACAGCGGGAAGTACTGCAGAGATTTTCTCAGGAGAGGAAAGCAAATGGGTCTCGGGATTTGTTTGCAAAGGCGTACGAATTGCTGTATTCGGGTTGCGGCGCTGCCGTGAGTGGCGGCAAGAGGAAGGTTTACAGAGGAGTGCGGCAGAGACAATGGGGGAAATGGGTGGCGGAGATTCGACTGCCGCAGAATCGGATGAGGGTGTGGTTAGGAACCTATGACTCGCCGGAGACCGCCGCCTATGCTTACGACTGCGCCGCCTGCAAACTCCGAGGGGAATATGCTCGGTTGAATTTTCCAAATTTGAAGGATCTAAAGAGCGATTTAGGTACCGACGAGTTTGTGAAATTGAGTGGGTTGAAAAAATTGGTGGATGCCAAAATTCAAGCGATTTTTCAGAAGATAAAGGGGAAGGGGAAGAAGAAGGATTGTCGGAGAATCGGCGGCGATTTAGGATCGGGTTCGTGTTCTTCGTCATCATCGTCGGTGTCTCTGCCTCCGGCGGTAGTGGAGATGACGGAAGAATGGAGTTGGGAAAGGGTATCGCCTGTTGGTGCAGCGGAAGATGGGTTGTGGAACTTTGAGAATTCGCCTCGAGCTGGTTCAGTGGATTGCTTGACGGCGACGGCGGCGGCGGGATCGGAAACAGAGTGGTACTCGTTGGAGAAGATGCCTTCTTTTGATGCAGAACAGATTTGGGATGTTCTTGCAAACTAG

Coding sequence (CDS)

ATGGAAACCATGACGAACGGAGGAACTCTTCAATTCACGGATGTTTCAACGGCTCTTTCCAATCTCATTCTAACTGCCGGAGGAAATACTCTGGATTCAATCTTCTCTCACTGCGACAATTTCAGCTGCTCTGTAATCGACGGCGTAGATTTCGACCCGGCAGTTGGGTCGTCGGTGTATCTCCGACAGCGGGAAGTACTGCAGAGATTTTCTCAGGAGAGGAAAGCAAATGGGTCTCGGGATTTGTTTGCAAAGGCGTACGAATTGCTGTATTCGGGTTGCGGCGCTGCCGTGAGTGGCGGCAAGAGGAAGGTTTACAGAGGAGTGCGGCAGAGACAATGGGGGAAATGGGTGGCGGAGATTCGACTGCCGCAGAATCGGATGAGGGTGTGGTTAGGAACCTATGACTCGCCGGAGACCGCCGCCTATGCTTACGACTGCGCCGCCTGCAAACTCCGAGGGGAATATGCTCGGTTGAATTTTCCAAATTTGAAGGATCTAAAGAGCGATTTAGGTACCGACGAGTTTGTGAAATTGAGTGGGTTGAAAAAATTGGTGGATGCCAAAATTCAAGCGATTTTTCAGAAGATAAAGGGGAAGGGGAAGAAGAAGGATTGTCGGAGAATCGGCGGCGATTTAGGATCGGGTTCGTGTTCTTCGTCATCATCGTCGGTGTCTCTGCCTCCGGCGGTAGTGGAGATGACGGAAGAATGGAGTTGGGAAAGGGTATCGCCTGTTGGTGCAGCGGAAGATGGGTTGTGGAACTTTGAGAATTCGCCTCGAGCTGGTTCAGTGGATTGCTTGACGGCGACGGCGGCGGCGGGATCGGAAACAGAGTGGTACTCGTTGGAGAAGATGCCTTCTTTTGATGCAGAACAGATTTGGGATGTTCTTGCAAACTAG

Protein sequence

METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVYLRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN
Homology
BLAST of Carg08566-RA vs. NCBI nr
Match: KAG7035483.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605.1 bits (1559), Expect = 3.2e-169
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60
           METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY
Sbjct: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60

Query: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120
           LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE
Sbjct: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120

Query: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180
           IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS
Sbjct: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180

Query: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240
           GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW
Sbjct: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240

Query: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN 300
           ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN
Sbjct: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN 300

BLAST of Carg08566-RA vs. NCBI nr
Match: XP_022958007.1 (ethylene-responsive transcription factor ERF061-like [Cucurbita moschata])

HSP 1 Score: 601.7 bits (1550), Expect = 3.5e-168
Identity = 298/300 (99.33%), Postives = 299/300 (99.67%), Query Frame = 0

Query: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60
           METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY
Sbjct: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60

Query: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120
           LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE
Sbjct: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120

Query: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180
           IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS
Sbjct: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180

Query: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240
           GL+KLVDAKIQAI QKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW
Sbjct: 181 GLRKLVDAKIQAIIQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240

Query: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN 300
           ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN
Sbjct: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN 300

BLAST of Carg08566-RA vs. NCBI nr
Match: XP_023534472.1 (ethylene-responsive transcription factor ERF061-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 581.6 bits (1498), Expect = 3.8e-162
Identity = 290/300 (96.67%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60
           METMTN GTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFS SVIDGVDF+PAVGSSVY
Sbjct: 1   METMTNRGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSFSVIDGVDFEPAVGSSVY 60

Query: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120
           LRQREVLQRFSQERKANGSRDLF KAYELLYSGCGAAV GGKRKVYRGVRQRQWGKWVAE
Sbjct: 61  LRQREVLQRFSQERKANGSRDLFTKAYELLYSGCGAAVDGGKRKVYRGVRQRQWGKWVAE 120

Query: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180
           IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLG+DEFVKLS
Sbjct: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGSDEFVKLS 180

Query: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240
           GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW
Sbjct: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240

Query: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN 300
           ERVSP+GAAEDGLWNFENSPRAGSVDCLTAT A GSETEWYSLEKMPSFDAEQIWDVLA+
Sbjct: 241 ERVSPIGAAEDGLWNFENSPRAGSVDCLTAT-ATGSETEWYSLEKMPSFDAEQIWDVLAS 299

BLAST of Carg08566-RA vs. NCBI nr
Match: XP_022995394.1 (ethylene-responsive transcription factor ERF061-like [Cucurbita maxima])

HSP 1 Score: 569.7 bits (1467), Expect = 1.5e-158
Identity = 286/299 (95.65%), Postives = 290/299 (96.99%), Query Frame = 0

Query: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60
           METMTN GTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFS SVIDGVDF+ AVGSSVY
Sbjct: 1   METMTNRGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSRSVIDGVDFEQAVGSSVY 60

Query: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120
           LRQREVLQRFSQERKANGSRDLFAKAYE+LYSGCGAAV GGKRKVYRGVRQRQWGKWVAE
Sbjct: 61  LRQREVLQRFSQERKANGSRDLFAKAYEVLYSGCGAAVGGGKRKVYRGVRQRQWGKWVAE 120

Query: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180
           IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLG+DEFVKLS
Sbjct: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGSDEFVKLS 180

Query: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240
           GLKKLVDAKIQAIFQKIKGKGKKKDCRRIG DLGSGSCSSSS SVSLPPAVVEMTE+WSW
Sbjct: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGSDLGSGSCSSSSPSVSLPPAVVEMTEKWSW 240

Query: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLA 300
           ERVS VGAAEDGLWNFENSPRAGSVDCLTAT A GSETEWYSLEKMPSFDAEQIWDVLA
Sbjct: 241 ERVSAVGAAEDGLWNFENSPRAGSVDCLTAT-ATGSETEWYSLEKMPSFDAEQIWDVLA 298

BLAST of Carg08566-RA vs. NCBI nr
Match: KAG7017013.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.8 bits (1187), Expect = 4.4e-126
Identity = 243/305 (79.67%), Postives = 261/305 (85.57%), Query Frame = 0

Query: 1   METMTNGGTLQF--TDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSS 60
           M TM N   LQF  TDVSTALSNLILT GGNTLDSIFSHC+N + SV DG DFD  VGSS
Sbjct: 1   MNTMMNRADLQFQLTDVSTALSNLILTGGGNTLDSIFSHCENLA-SVNDGGDFDRLVGSS 60

Query: 61  VYLRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWV 120
           VYL+QREVL+RFSQ+ K NGSRDLF++AYELLYS  GAAV GG+RK+YRGVRQR WGKWV
Sbjct: 61  VYLQQREVLKRFSQDSKPNGSRDLFSRAYELLYSRSGAAVGGGERKLYRGVRQRHWGKWV 120

Query: 121 AEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVK 180
           AEIRLPQNRMRVWLGTYDSPE AAYAYDCAACKLRGEYARLNFPNLK+LK+DLG  EF +
Sbjct: 121 AEIRLPQNRMRVWLGTYDSPEAAAYAYDCAACKLRGEYARLNFPNLKELKTDLGAGEFAR 180

Query: 181 LSGLKKLVDAKIQAIFQKI-KGKGKKK-DCRRIGGDLGSGSC-SSSSSSVSLPPAVVEMT 240
           LSGLKKLVDAKIQAIFQKI KGKGKK    +RIG DLGSGSC SSSSSSVSLPPA VE+T
Sbjct: 181 LSGLKKLVDAKIQAIFQKIRKGKGKKSVKKQRIGSDLGSGSCSSSSSSSVSLPPAAVELT 240

Query: 241 EEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIW 300
           +EWSWERV P  AAE+GLWNFENSPR  S DC TATA AG ETE YSL KMPS+DAE IW
Sbjct: 241 DEWSWERVQPPVAAEEGLWNFENSPRDVSTDCATATATAGLETECYSLAKMPSYDAELIW 300

BLAST of Carg08566-RA vs. ExPASy Swiss-Prot
Match: Q9C7W2 (Ethylene-responsive transcription factor ERF061 OS=Arabidopsis thaliana OX=3702 GN=ERF061 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.8e-53
Identity = 147/334 (44.01%), Postives = 191/334 (57.19%), Query Frame = 0

Query: 6   NGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFD-------PAVGSS 65
           N  + + +++  +LS +IL  G NTLDSIFS     S        F+       P +GSS
Sbjct: 10  NNFSPKISEIRASLSQIILAGGPNTLDSIFSLLTPSSVESAT-TSFNTHNPPPPPQLGSS 69

Query: 66  VYLRQREVLQRFSQERKANGS--RDLFAKAYE-------LLYSGCG-------AAVSGG- 125
           VYLRQR+++++F  + +A  +    LF+  Y+       +L +  G       AA++ G 
Sbjct: 70  VYLRQRDIIEKFHLQNRAISTPHPPLFSSTYDHHQTSELMLQAAAGSPAAAFAAALAAGR 129

Query: 126 ---KRKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYAR 185
              K+K+YRGVRQR WGKWVAEIRLPQNRMRVWLGTYD+ E AAYAYD AA KLRGEYAR
Sbjct: 130 VTKKKKLYRGVRQRHWGKWVAEIRLPQNRMRVWLGTYDTAEAAAYAYDRAAYKLRGEYAR 189

Query: 186 LNFPNLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCR--RIGGDLGSG 245
           LNFPNLKD    LG  +  KL  LK  VD KIQ+I Q+++ +  KK  +  +        
Sbjct: 190 LNFPNLKDPSELLGLGDSSKLIALKNAVDGKIQSICQRVRKERAKKSVKVSKNSSATADS 249

Query: 246 SCSSSSSSVSLPPAVVEMT----EEWSWERVSPVGAAEDGLWNFEN-SPRAGSVDC-LTA 301
           SC SS   +S  P     T    E+  W  VSP+     GL N EN SP + SV   + A
Sbjct: 250 SCLSSPEILSSSPVTTTTTAVTSEDSYW--VSPM-----GLCNSENSSPVSVSVPSEVPA 309

BLAST of Carg08566-RA vs. ExPASy Swiss-Prot
Match: Q8H1E4 (Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana OX=3702 GN=RAP2-4 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 7.5e-28
Identity = 87/206 (42.23%), Postives = 113/206 (54.85%), Query Frame = 0

Query: 95  GAAVSGGKRKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRG 154
           G+ V     K+YRGVRQR WGKWVAEIRLP+NR R+WLGT+D+ E AA AYD AA KLRG
Sbjct: 141 GSGVPSKPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRG 200

Query: 155 EYARLNFPNLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLG 214
           ++ARLNFPNL+   S +G D F +   L   VDAK++AI + +    K+        D  
Sbjct: 201 DFARLNFPNLRHNGSHIGGD-FGEYKPLHSSVDAKLEAICKSMAETQKQ--------DKS 260

Query: 215 SGSCSSSSSSVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAA 274
           + S       VS P    +++E+   E  S        +  FE S  AGS      T A 
Sbjct: 261 TKSSKKREKKVSSP----DLSEKVKAEENSVSIGGSPPVTEFEES-TAGSSPLSDLTFAD 320

Query: 275 GSE----TEWYSLEKMPSFDAEQIWD 297
             E     E +SLEK PS++ +  WD
Sbjct: 321 PEEPPQWNETFSLEKYPSYEID--WD 330

BLAST of Carg08566-RA vs. ExPASy Swiss-Prot
Match: O65665 (Ethylene-responsive transcription factor ERF060 OS=Arabidopsis thaliana OX=3702 GN=ERF060 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 7.7e-25
Identity = 79/197 (40.10%), Postives = 105/197 (53.30%), Query Frame = 0

Query: 103 RKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFP 162
           +K+YRGVRQR WGKWVAEIRLP+NR R+WLGT+D+ E AA AYD AA KLRGE+ARLNFP
Sbjct: 90  QKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAMAYDLAAYKLRGEFARLNFP 149

Query: 163 NLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSS 222
             +      G       + L   VDAK+Q I Q ++   K +D      DL    CS + 
Sbjct: 150 QFRHEDGYYGGGSC--FNPLHSSVDAKLQEICQSLR---KTEDI-----DL---PCSETE 209

Query: 223 SSVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYS 282
                PP       E+ + R S   +  D      +SP +G    L  + +   E   + 
Sbjct: 210 ---LFPPKTEYQESEYGFLR-SDENSFSDESHVESSSPESGITTFLDFSDSGFDEIGSFG 267

Query: 283 LEKMPSFDAEQIWDVLA 300
           LEK PS + +  WD ++
Sbjct: 270 LEKFPSVEID--WDAIS 267

BLAST of Carg08566-RA vs. ExPASy Swiss-Prot
Match: Q9LM15 (Ethylene-responsive transcription factor RAP2-13 OS=Arabidopsis thaliana OX=3702 GN=RAP2-13 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.2e-23
Identity = 87/224 (38.84%), Postives = 116/224 (51.79%), Query Frame = 0

Query: 79  SRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSP 138
           S  L  K   +  +G  AA      K+YRGVRQR WGKWVAEIRLP+NR R+WLGT+D+ 
Sbjct: 59  SHHLSPKPVSMKQTGTSAA---KPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTA 118

Query: 139 ETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIK 198
           E AA AYD AA KLRG++ARLNFP+L+        DE+     L+  VDAK++AI Q + 
Sbjct: 119 EEAALAYDKAAYKLRGDFARLNFPDLRH------NDEY---QPLQSSVDAKLEAICQNLA 178

Query: 199 GKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFEN 258
              +K+            +  SSS   S   AV    E++S    SP+          E+
Sbjct: 179 ETTQKQ---------VRSTKKSSSRKRSSTVAVKLPEEDYSSAGSSPLLT--------ES 238

Query: 259 SPRAGSVDCLTATAAAGSETE----W--YSLEKMPSFDAEQIWD 297
               GS   L+      +E E    W   +LEK PS++ +  WD
Sbjct: 239 YGSGGSSSPLSELTFGDTEEEIQPPWNENALEKYPSYEID--WD 251

BLAST of Carg08566-RA vs. ExPASy Swiss-Prot
Match: Q9SVQ0 (Ethylene-responsive transcription factor ERF062 OS=Arabidopsis thaliana OX=3702 GN=ERF062 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.6e-22
Identity = 73/196 (37.24%), Postives = 99/196 (50.51%), Query Frame = 0

Query: 104 KVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPN 163
           K+YRGVRQR WGKWVAEIRLP+NR RVWLGT+++ E AA AYD AA  LRGE+A LNFP+
Sbjct: 230 KLYRGVRQRHWGKWVAEIRLPRNRTRVWLGTFETAEQAAMAYDTAAYILRGEFAHLNFPD 289

Query: 164 LKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSS 223
           LK           +K   L+ ++ + +++  Q+I                   S S  S+
Sbjct: 290 LK---------HQLKSGSLRCMIASLLESKIQQI-------------------SSSQVSN 349

Query: 224 SVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSL 283
           S S PP  V   E+ +       G  ED +   + S +               E +   L
Sbjct: 350 SPSPPPPKVGTPEQKNHHMKMESG--EDVMMKKQKSHK------------EVMEGDGVQL 383

Query: 284 EKMPSFDAEQIWDVLA 300
            +MPS D + IWD L+
Sbjct: 410 SRMPSLDMDLIWDALS 383

BLAST of Carg08566-RA vs. ExPASy TrEMBL
Match: A0A6J1H0R0 (ethylene-responsive transcription factor ERF061-like OS=Cucurbita moschata OX=3662 GN=LOC111459370 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.7e-168
Identity = 298/300 (99.33%), Postives = 299/300 (99.67%), Query Frame = 0

Query: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60
           METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY
Sbjct: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60

Query: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120
           LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE
Sbjct: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120

Query: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180
           IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS
Sbjct: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180

Query: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240
           GL+KLVDAKIQAI QKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW
Sbjct: 181 GLRKLVDAKIQAIIQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240

Query: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN 300
           ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN
Sbjct: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLAN 300

BLAST of Carg08566-RA vs. ExPASy TrEMBL
Match: A0A6J1K5K2 (ethylene-responsive transcription factor ERF061-like OS=Cucurbita maxima OX=3661 GN=LOC111490954 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 7.2e-159
Identity = 286/299 (95.65%), Postives = 290/299 (96.99%), Query Frame = 0

Query: 1   METMTNGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVY 60
           METMTN GTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFS SVIDGVDF+ AVGSSVY
Sbjct: 1   METMTNRGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSRSVIDGVDFEQAVGSSVY 60

Query: 61  LRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAE 120
           LRQREVLQRFSQERKANGSRDLFAKAYE+LYSGCGAAV GGKRKVYRGVRQRQWGKWVAE
Sbjct: 61  LRQREVLQRFSQERKANGSRDLFAKAYEVLYSGCGAAVGGGKRKVYRGVRQRQWGKWVAE 120

Query: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLS 180
           IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLG+DEFVKLS
Sbjct: 121 IRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGSDEFVKLS 180

Query: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSW 240
           GLKKLVDAKIQAIFQKIKGKGKKKDCRRIG DLGSGSCSSSS SVSLPPAVVEMTE+WSW
Sbjct: 181 GLKKLVDAKIQAIFQKIKGKGKKKDCRRIGSDLGSGSCSSSSPSVSLPPAVVEMTEKWSW 240

Query: 241 ERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIWDVLA 300
           ERVS VGAAEDGLWNFENSPRAGSVDCLTAT A GSETEWYSLEKMPSFDAEQIWDVLA
Sbjct: 241 ERVSAVGAAEDGLWNFENSPRAGSVDCLTAT-ATGSETEWYSLEKMPSFDAEQIWDVLA 298

BLAST of Carg08566-RA vs. ExPASy TrEMBL
Match: A0A5A7TL46 (Ethylene-responsive transcription factor ERF061-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001900 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.2e-121
Identity = 236/306 (77.12%), Postives = 257/306 (83.99%), Query Frame = 0

Query: 4   MTNGGTLQF--TDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVYL 63
           M N   LQF  TDVSTALSNLILT GGNTLDSI SH ++ S SV D  DF PA GSSVYL
Sbjct: 1   MINTPNLQFGLTDVSTALSNLILTGGGNTLDSILSHSESLS-SVTDSRDFQPAAGSSVYL 60

Query: 64  RQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAEI 123
           +QREVLQ+FSQ+RK+NGSRDLF++AYELLYS  GAAV+GG+RK+YRGVRQR WGKWVAEI
Sbjct: 61  QQREVLQKFSQDRKSNGSRDLFSRAYELLYSRSGAAVNGGERKIYRGVRQRHWGKWVAEI 120

Query: 124 RLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLSG 183
           RLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLK+DL + EF +LS 
Sbjct: 121 RLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKTDLSSGEFARLSE 180

Query: 184 LKKLVDAKIQAIFQKI-KGKG----KKKDCRRIGGDLG--SGSCSSSSSSVSLPPAVVEM 243
           LKK+VDAKIQAIFQKI KGKG    KKKD +R GGDLG  SGSCSSSSSSVSLPPA  E+
Sbjct: 181 LKKMVDAKIQAIFQKIRKGKGKKSVKKKDSQRTGGDLGSISGSCSSSSSSVSLPPAAAEL 240

Query: 244 TEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQI 301
           TEEWSWE V P   AE+GLWNFENSP+A S+DC    A AGSETE YSL KMPSFDA+ I
Sbjct: 241 TEEWSWENVQPPATAEEGLWNFENSPKAVSMDC----AMAGSETECYSLAKMPSFDADLI 300

BLAST of Carg08566-RA vs. ExPASy TrEMBL
Match: A0A1S3AU14 (ethylene-responsive transcription factor ERF061-like OS=Cucumis melo OX=3656 GN=LOC103482818 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.2e-121
Identity = 236/306 (77.12%), Postives = 257/306 (83.99%), Query Frame = 0

Query: 4   MTNGGTLQF--TDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSSVYL 63
           M N   LQF  TDVSTALSNLILT GGNTLDSI SH ++ S SV D  DF PA GSSVYL
Sbjct: 1   MINTPNLQFGLTDVSTALSNLILTGGGNTLDSILSHSESLS-SVTDSRDFQPAAGSSVYL 60

Query: 64  RQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAEI 123
           +QREVLQ+FSQ+RK+NGSRDLF++AYELLYS  GAAV+GG+RK+YRGVRQR WGKWVAEI
Sbjct: 61  QQREVLQKFSQDRKSNGSRDLFSRAYELLYSRSGAAVNGGERKIYRGVRQRHWGKWVAEI 120

Query: 124 RLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLSG 183
           RLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLK+DL + EF +LS 
Sbjct: 121 RLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKTDLSSGEFARLSE 180

Query: 184 LKKLVDAKIQAIFQKI-KGKG----KKKDCRRIGGDLG--SGSCSSSSSSVSLPPAVVEM 243
           LKK+VDAKIQAIFQKI KGKG    KKKD +R GGDLG  SGSCSSSSSSVSLPPA  E+
Sbjct: 181 LKKMVDAKIQAIFQKIRKGKGKKSVKKKDSQRTGGDLGSISGSCSSSSSSVSLPPAAAEL 240

Query: 244 TEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQI 301
           TEEWSWE V P   AE+GLWNFENSP+A S+DC    A AGSETE YSL KMPSFDA+ I
Sbjct: 241 TEEWSWENVQPPATAEEGLWNFENSPKAVSMDC----AMAGSETECYSLAKMPSFDADLI 300

BLAST of Carg08566-RA vs. ExPASy TrEMBL
Match: A0A6J1ETF8 (ethylene-responsive transcription factor ERF061-like OS=Cucurbita moschata OX=3662 GN=LOC111435813 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.1e-121
Identity = 235/305 (77.05%), Postives = 254/305 (83.28%), Query Frame = 0

Query: 1   METMTNGGTLQF--TDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFDPAVGSS 60
           M TM N   LQF  TDVSTALSNLILT GGNTLDSIFSHC+N           D  VGSS
Sbjct: 1   MNTMMNRADLQFQLTDVSTALSNLILTGGGNTLDSIFSHCEN----------LDRPVGSS 60

Query: 61  VYLRQREVLQRFSQERKANGSRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWV 120
           VYL++REVL+RFSQ+ + NGSRDLF++AYELLYS  GAAV GG+RK+YRGVRQR WGKWV
Sbjct: 61  VYLQKREVLKRFSQDSRPNGSRDLFSRAYELLYSRSGAAVGGGERKLYRGVRQRHWGKWV 120

Query: 121 AEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVK 180
           AEIRLPQNRMRVWLGTYDSPE AAYAYDCAACKLRGEYARLNFPNLK+LK+DLG  EF +
Sbjct: 121 AEIRLPQNRMRVWLGTYDSPEAAAYAYDCAACKLRGEYARLNFPNLKELKTDLGAGEFAR 180

Query: 181 LSGLKKLVDAKIQAIFQKI-KGKGKKK-DCRRIGGDLGSGSC-SSSSSSVSLPPAVVEMT 240
           LSGLKKLVDAKIQAIFQKI KGKGKK    +RIG DLGSGSC SSSSSSVSLPPA VE+T
Sbjct: 181 LSGLKKLVDAKIQAIFQKIRKGKGKKSVKKQRIGSDLGSGSCSSSSSSSVSLPPAAVELT 240

Query: 241 EEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSLEKMPSFDAEQIW 300
           +EWSWERV P  AAE+GLWNFENSPR  S DC TATA AG ETE YSL KMPS+DAE IW
Sbjct: 241 DEWSWERVQPPVAAEEGLWNFENSPRDVSTDCATATATAGLETECYSLAKMPSYDAELIW 295

BLAST of Carg08566-RA vs. TAIR 10
Match: AT1G64380.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 208.8 bits (530), Expect = 6.2e-54
Identity = 147/334 (44.01%), Postives = 191/334 (57.19%), Query Frame = 0

Query: 6   NGGTLQFTDVSTALSNLILTAGGNTLDSIFSHCDNFSCSVIDGVDFD-------PAVGSS 65
           N  + + +++  +LS +IL  G NTLDSIFS     S        F+       P +GSS
Sbjct: 10  NNFSPKISEIRASLSQIILAGGPNTLDSIFSLLTPSSVESAT-TSFNTHNPPPPPQLGSS 69

Query: 66  VYLRQREVLQRFSQERKANGS--RDLFAKAYE-------LLYSGCG-------AAVSGG- 125
           VYLRQR+++++F  + +A  +    LF+  Y+       +L +  G       AA++ G 
Sbjct: 70  VYLRQRDIIEKFHLQNRAISTPHPPLFSSTYDHHQTSELMLQAAAGSPAAAFAAALAAGR 129

Query: 126 ---KRKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYAR 185
              K+K+YRGVRQR WGKWVAEIRLPQNRMRVWLGTYD+ E AAYAYD AA KLRGEYAR
Sbjct: 130 VTKKKKLYRGVRQRHWGKWVAEIRLPQNRMRVWLGTYDTAEAAAYAYDRAAYKLRGEYAR 189

Query: 186 LNFPNLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCR--RIGGDLGSG 245
           LNFPNLKD    LG  +  KL  LK  VD KIQ+I Q+++ +  KK  +  +        
Sbjct: 190 LNFPNLKDPSELLGLGDSSKLIALKNAVDGKIQSICQRVRKERAKKSVKVSKNSSATADS 249

Query: 246 SCSSSSSSVSLPPAVVEMT----EEWSWERVSPVGAAEDGLWNFEN-SPRAGSVDC-LTA 301
           SC SS   +S  P     T    E+  W  VSP+     GL N EN SP + SV   + A
Sbjct: 250 SCLSSPEILSSSPVTTTTTAVTSEDSYW--VSPM-----GLCNSENSSPVSVSVPSEVPA 309

BLAST of Carg08566-RA vs. TAIR 10
Match: AT1G78080.1 (related to AP2 4 )

HSP 1 Score: 125.9 bits (315), Expect = 5.3e-29
Identity = 87/206 (42.23%), Postives = 113/206 (54.85%), Query Frame = 0

Query: 95  GAAVSGGKRKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRG 154
           G+ V     K+YRGVRQR WGKWVAEIRLP+NR R+WLGT+D+ E AA AYD AA KLRG
Sbjct: 141 GSGVPSKPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRG 200

Query: 155 EYARLNFPNLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLG 214
           ++ARLNFPNL+   S +G D F +   L   VDAK++AI + +    K+        D  
Sbjct: 201 DFARLNFPNLRHNGSHIGGD-FGEYKPLHSSVDAKLEAICKSMAETQKQ--------DKS 260

Query: 215 SGSCSSSSSSVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAA 274
           + S       VS P    +++E+   E  S        +  FE S  AGS      T A 
Sbjct: 261 TKSSKKREKKVSSP----DLSEKVKAEENSVSIGGSPPVTEFEES-TAGSSPLSDLTFAD 320

Query: 275 GSE----TEWYSLEKMPSFDAEQIWD 297
             E     E +SLEK PS++ +  WD
Sbjct: 321 PEEPPQWNETFSLEKYPSYEID--WD 330

BLAST of Carg08566-RA vs. TAIR 10
Match: AT4G39780.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 5.5e-26
Identity = 79/197 (40.10%), Postives = 105/197 (53.30%), Query Frame = 0

Query: 103 RKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFP 162
           +K+YRGVRQR WGKWVAEIRLP+NR R+WLGT+D+ E AA AYD AA KLRGE+ARLNFP
Sbjct: 90  QKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAMAYDLAAYKLRGEFARLNFP 149

Query: 163 NLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSS 222
             +      G       + L   VDAK+Q I Q ++   K +D      DL    CS + 
Sbjct: 150 QFRHEDGYYGGGSC--FNPLHSSVDAKLQEICQSLR---KTEDI-----DL---PCSETE 209

Query: 223 SSVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYS 282
                PP       E+ + R S   +  D      +SP +G    L  + +   E   + 
Sbjct: 210 ---LFPPKTEYQESEYGFLR-SDENSFSDESHVESSSPESGITTFLDFSDSGFDEIGSFG 267

Query: 283 LEKMPSFDAEQIWDVLA 300
           LEK PS + +  WD ++
Sbjct: 270 LEKFPSVEID--WDAIS 267

BLAST of Carg08566-RA vs. TAIR 10
Match: AT1G22190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 3.0e-24
Identity = 87/224 (38.84%), Postives = 116/224 (51.79%), Query Frame = 0

Query: 79  SRDLFAKAYELLYSGCGAAVSGGKRKVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSP 138
           S  L  K   +  +G  AA      K+YRGVRQR WGKWVAEIRLP+NR R+WLGT+D+ 
Sbjct: 59  SHHLSPKPVSMKQTGTSAA---KPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTA 118

Query: 139 ETAAYAYDCAACKLRGEYARLNFPNLKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIK 198
           E AA AYD AA KLRG++ARLNFP+L+        DE+     L+  VDAK++AI Q + 
Sbjct: 119 EEAALAYDKAAYKLRGDFARLNFPDLRH------NDEY---QPLQSSVDAKLEAICQNLA 178

Query: 199 GKGKKKDCRRIGGDLGSGSCSSSSSSVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFEN 258
              +K+            +  SSS   S   AV    E++S    SP+          E+
Sbjct: 179 ETTQKQ---------VRSTKKSSSRKRSSTVAVKLPEEDYSSAGSSPLLT--------ES 238

Query: 259 SPRAGSVDCLTATAAAGSETE----W--YSLEKMPSFDAEQIWD 297
               GS   L+      +E E    W   +LEK PS++ +  WD
Sbjct: 239 YGSGGSSSPLSELTFGDTEEEIQPPWNENALEKYPSYEID--WD 251

BLAST of Carg08566-RA vs. TAIR 10
Match: AT4G13620.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-23
Identity = 73/196 (37.24%), Postives = 99/196 (50.51%), Query Frame = 0

Query: 104 KVYRGVRQRQWGKWVAEIRLPQNRMRVWLGTYDSPETAAYAYDCAACKLRGEYARLNFPN 163
           K+YRGVRQR WGKWVAEIRLP+NR RVWLGT+++ E AA AYD AA  LRGE+A LNFP+
Sbjct: 230 KLYRGVRQRHWGKWVAEIRLPRNRTRVWLGTFETAEQAAMAYDTAAYILRGEFAHLNFPD 289

Query: 164 LKDLKSDLGTDEFVKLSGLKKLVDAKIQAIFQKIKGKGKKKDCRRIGGDLGSGSCSSSSS 223
           LK           +K   L+ ++ + +++  Q+I                   S S  S+
Sbjct: 290 LK---------HQLKSGSLRCMIASLLESKIQQI-------------------SSSQVSN 349

Query: 224 SVSLPPAVVEMTEEWSWERVSPVGAAEDGLWNFENSPRAGSVDCLTATAAAGSETEWYSL 283
           S S PP  V   E+ +       G  ED +   + S +               E +   L
Sbjct: 350 SPSPPPPKVGTPEQKNHHMKMESG--EDVMMKKQKSHK------------EVMEGDGVQL 383

Query: 284 EKMPSFDAEQIWDVLA 300
            +MPS D + IWD L+
Sbjct: 410 SRMPSLDMDLIWDALS 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035483.13.2e-169100.00Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp.... [more]
XP_022958007.13.5e-16899.33ethylene-responsive transcription factor ERF061-like [Cucurbita moschata][more]
XP_023534472.13.8e-16296.67ethylene-responsive transcription factor ERF061-like [Cucurbita pepo subsp. pepo... [more]
XP_022995394.11.5e-15895.65ethylene-responsive transcription factor ERF061-like [Cucurbita maxima][more]
KAG7017013.14.4e-12679.67Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
Q9C7W28.8e-5344.01Ethylene-responsive transcription factor ERF061 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8H1E47.5e-2842.23Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana OX=3702 ... [more]
O656657.7e-2540.10Ethylene-responsive transcription factor ERF060 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LM154.2e-2338.84Ethylene-responsive transcription factor RAP2-13 OS=Arabidopsis thaliana OX=3702... [more]
Q9SVQ01.6e-2237.24Ethylene-responsive transcription factor ERF062 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1H0R01.7e-16899.33ethylene-responsive transcription factor ERF061-like OS=Cucurbita moschata OX=36... [more]
A0A6J1K5K27.2e-15995.65ethylene-responsive transcription factor ERF061-like OS=Cucurbita maxima OX=3661... [more]
A0A5A7TL461.2e-12177.12Ethylene-responsive transcription factor ERF061-like protein OS=Cucumis melo var... [more]
A0A1S3AU141.2e-12177.12ethylene-responsive transcription factor ERF061-like OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1ETF82.1e-12177.05ethylene-responsive transcription factor ERF061-like OS=Cucurbita moschata OX=36... [more]
Match NameE-valueIdentityDescription
AT1G64380.16.2e-5444.01Integrase-type DNA-binding superfamily protein [more]
AT1G78080.15.3e-2942.23related to AP2 4 [more]
AT4G39780.15.5e-2640.10Integrase-type DNA-binding superfamily protein [more]
AT1G22190.13.0e-2438.84Integrase-type DNA-binding superfamily protein [more]
AT4G13620.11.1e-2337.24Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 144..164
score: 54.98
coord: 106..117
score: 72.42
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 105..168
e-value: 1.1E-33
score: 127.9
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 105..155
e-value: 3.8E-14
score: 52.7
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 105..162
score: 22.444565
IPR001471AP2/ERF domainCDDcd00018AP2coord: 106..164
e-value: 7.43678E-20
score: 79.6231
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 105..163
e-value: 1.2E-24
score: 88.2
NoneNo IPR availablePANTHERPTHR31657ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061coord: 10..300
NoneNo IPR availablePANTHERPTHR31657:SF20ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061coord: 10..300
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 105..162

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg08566Carg08566gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08566-RA:1Carg08566-RA:1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08566-RA:cdsCarg08566-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg08566-RACarg08566-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity