Carg08517-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08517-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Alpha-L-fucosidase
Location	Carg_Chr02: 5220390 .. 5221379 (+)
Sequence length	981
RNA-Seq Expression	Carg08517-RA
Synteny	Carg08517-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAAATGGGTCACCGTTTTTCCTTCATCTTCACCCTCACATTGCTCCATTTTCTCCATTTCTGTTCTGCAAAGCTCGGGGTGTCAACGCCGCCGCCATTGCCAATCCTCCCACTCCCTTCTTTCTCCCAATTGAAATGGCAACAGAGAGAAGTGATTATGTTCCTCCACTTTGGGCCCAACACTTTCACCGATTCCGAGTGGGGCACCGGCCGCGAGTCTCCCACCGTCTTCAACCCCACAGCCCTCGACGCAGCACAGTGGGCAGCCACCGCCGCTGCCGCTAACATTTCTCTCATGATTCTCACTGCTAAGCACCACGACGGCTTCTGCCTCTGGCCTTCTAAATACACCACCCACTCTGTTTCCCGCAGCCCTTGGAAGAATGGCCACGGCGACGTCGTTAAGGAGTTCTACTACAACTCAGTGGGAAGAAATTGTGTTCTTCTCTTCAATGTCCCGCCCAACTCCACAGGTCTAATTGCCCAAGAAGATGCCCACACTCTCACCCAATTCAAAGCTGCCATACACACAATTTTCTCCTCAAATTTGGCTCAAAATTGCTCCTTAAGAGCCAGTAGCCAAAGGGGTGGCTAAGTAAGTGCGTTTGGGCCTGAAAATGTGTTGGACAATGACCATTGGTGGACTTATTGGGCACCCAGGGGAGCTGATGCTGATGATGATGCTGGTGGTGACCATTGGGTTGAGATCAGAAGTCAGCAGAAGCAAGGGCTGAGATTCAATGTGGTGAGGATCCAAGAGGCCATAGGGCTTGGTCAGAGGATCAAACGGCACGAGATTTATTTGGATGTGAAGAGGATTGTGGAGGAGAGTGGCGTCGGGTATAAGCGGCTGCATAGGATCAAAAGTGGAGTGGTTTGTGGGGAAGTTGTGAGGGTTAGGATCACCGAGGCTAGGGCTGTTCCTTTGATCTCCTCTTTGGGTCTCCATTTGGACCCTTTTTGGGACCCAACTGGGCTTTCATGA

mRNA sequence

ATGGCAGAAATGGGTCACCGTTTTTCCTTCATCTTCACCCTCACATTGCTCCATTTTCTCCATTTCTGTTCTGCAAAGCTCGGGGTGTCAACGCCGCCGCCATTGCCAATCCTCCCACTCCCTTCTTTCTCCCAATTGAAATGGCAACAGAGAGAAGTGATTATGTTCCTCCACTTTGGGCCCAACACTTTCACCGATTCCGAGTGGGGCACCGGCCGCGAGTCTCCCACCGTCTTCAACCCCACAGCCCTCGACGCAGCACAGTGGGCAGCCACCGCCGCTGCCGCTAACATTTCTCTCATGATTCTCACTGCTAAGCACCACGACGGCTTCTGCCTCTGGCCTTCTAAATACACCACCCACTCTGTTTCCCGCAGCCCTTGGAAGAATGGCCACGGCGACGTCGTTAAGGAGTTCTACTACAACTCAGTGGGAAGAAATTGTGTTCTTCTCTTCAATGTCCCGCCCAACTCCACAGGTCTAATTGCCCAAGAAGATGCCCACACTCTCACCCAATTCAAAGCTGCCATACACACAATTTTCTCCTCAAATTTGGCTCAAAATTGCTCCTTAAGAGCCAGTAGCCAAAGGGTAAGTGCGTTTGGGCCTGAAAATGTGTTGGACAATGACCATTGGTGGACTTATTGGGCACCCAGGGGAGCTGATGCTGATGATGATGCTGGTGGTGACCATTGGGTTGAGATCAGAAGTCAGCAGAAGCAAGGGCTGAGATTCAATGTGGTGAGGATCCAAGAGGCCATAGGGCTTGGTCAGAGGATCAAACGGCACGAGATTTATTTGGATGTGAAGAGGATTGTGGAGGAGAGTGGCGTCGGGTATAAGCGGCTGCATAGGATCAAAAGTGGAGTGGTTTGTGGGGAAGTTGTGAGGGTTAGGATCACCGAGGCTAGGGCTGTTCCTTTGATCTCCTCTTTGGGTCTCCATTTGGACCCTTTTTGGGACCCAACTGGGCTTTCATGA

Coding sequence (CDS)

ATGGCAGAAATGGGTCACCGTTTTTCCTTCATCTTCACCCTCACATTGCTCCATTTTCTCCATTTCTGTTCTGCAAAGCTCGGGGTGTCAACGCCGCCGCCATTGCCAATCCTCCCACTCCCTTCTTTCTCCCAATTGAAATGGCAACAGAGAGAAGTGATTATGTTCCTCCACTTTGGGCCCAACACTTTCACCGATTCCGAGTGGGGCACCGGCCGCGAGTCTCCCACCGTCTTCAACCCCACAGCCCTCGACGCAGCACAGTGGGCAGCCACCGCCGCTGCCGCTAACATTTCTCTCATGATTCTCACTGCTAAGCACCACGACGGCTTCTGCCTCTGGCCTTCTAAATACACCACCCACTCTGTTTCCCGCAGCCCTTGGAAGAATGGCCACGGCGACGTCGTTAAGGAGTTCTACTACAACTCAGTGGGAAGAAATTGTGTTCTTCTCTTCAATGTCCCGCCCAACTCCACAGGTCTAATTGCCCAAGAAGATGCCCACACTCTCACCCAATTCAAAGCTGCCATACACACAATTTTCTCCTCAAATTTGGCTCAAAATTGCTCCTTAAGAGCCAGTAGCCAAAGGGTAAGTGCGTTTGGGCCTGAAAATGTGTTGGACAATGACCATTGGTGGACTTATTGGGCACCCAGGGGAGCTGATGCTGATGATGATGCTGGTGGTGACCATTGGGTTGAGATCAGAAGTCAGCAGAAGCAAGGGCTGAGATTCAATGTGGTGAGGATCCAAGAGGCCATAGGGCTTGGTCAGAGGATCAAACGGCACGAGATTTATTTGGATGTGAAGAGGATTGTGGAGGAGAGTGGCGTCGGGTATAAGCGGCTGCATAGGATCAAAAGTGGAGTGGTTTGTGGGGAAGTTGTGAGGGTTAGGATCACCGAGGCTAGGGCTGTTCCTTTGATCTCCTCTTTGGGTCTCCATTTGGACCCTTTTTGGGACCCAACTGGGCTTTCATGA

Protein sequence

MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTTHSVSRSPWKNGHGDVVKEFYYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRASSQRVSAFGPENVLDNDHWWTYWAPRGADADDDAGGDHWVEIRSQQKQGLRFNVVRIQEAIGLGQRIKRHEIYLDVKRIVEESGVGYKRLHRIKSGVVCGEVVRVRITEARAVPLISSLGLHLDPFWDPTGLS

Homology

[Close]

BLAST of Carg08517-RA vs. NCBI nr
Match: XP_022958355.1 (putative alpha-L-fucosidase 1 [Cucurbita moschata])

HSP 1 Score: 578.6 bits (1490), Expect = 3.5e-161
Identity = 317/496 (63.91%), Postives = 318/496 (64.11%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSVSRSPWKNGHGDVVKEF                                         
Sbjct: 121 HSVSRSPWKNGHGDVVKEFVDAAAPRGIDVGVYLSPWDRHDRRYSHELAYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLNNYGNVREIWFDGAKGKNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDIRWVGDENG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGTPCWSTINRSSLAIGAEGITQYLNEGDPEGTHWVPPECDVSIRNGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 327
                 YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA
Sbjct: 301 KTLLEIYYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 360

[Close]

BLAST of Carg08517-RA vs. NCBI nr
Match: XP_023534414.1 (putative alpha-L-fucosidase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 573.2 bits (1476), Expect = 1.5e-159
Identity = 315/498 (63.25%), Postives = 318/498 (63.86%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           M EMGH FSFIFTLTLLHFLHFCSAKLGVST PPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MVEMGHCFSFIFTLTLLHFLHFCSAKLGVSTAPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTGRESPTVFNPTAL+AAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGRESPTVFNPTALNAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSVSRSPWKNGHGDVVKEF                                         
Sbjct: 121 HSVSRSPWKNGHGDVVKEFVDAAAARGIDVGVYLSPWDRHDRRYSHELAYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLNNYGNVREIWFDGAKGKNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDIRWVGDENG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGTPCWSTINRSSLAIGAQGITRYLNEGDPEGTHWIPPECDVSIRKGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 327
                 YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA
Sbjct: 301 KTLLEIYYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 360

[Close]

BLAST of Carg08517-RA vs. NCBI nr
Match: XP_022929122.1 (alpha-L-fucosidase 1-like [Cucurbita moschata])

HSP 1 Score: 483.8 bits (1244), Expect = 1.2e-132
Identity = 272/495 (54.95%), Postives = 289/495 (58.38%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           MAE GH FS IF LTLLH LHF S KL VSTPPPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MAEKGHLFSSIFFLTLLHILHFSSPKLEVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTG ESP +FNPT+L+AAQWAATAAAA ISLMILTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGAESPALFNPTSLNAAQWAATAAAAGISLMILTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSV RSPWK+G GDVVKEF                                         
Sbjct: 121 HSVVRSPWKDGRGDVVKEFVTAAAAHGVDVGLYLSPWDRHDRRYSHDLPYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLNKYGNVREIWFDGAKGQNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDVRWVGNEKG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGTPCWSTINRTLLAIGAEGISEYLNKGDPKGTHWVPPECDVSIRKGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 326
                 YYNSVGRNCVLL NVPPNSTGLIAQEDA TL QFKAAI TIF++NLA NCS++A
Sbjct: 301 NELLKIYYNSVGRNCVLLVNVPPNSTGLIAQEDADTLKQFKAAIDTIFTTNLAHNCSVKA 360

[Close]

BLAST of Carg08517-RA vs. NCBI nr
Match: KAG6579606.1 (Alpha-L-fucosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483.4 bits (1243), Expect = 1.5e-132
Identity = 271/495 (54.75%), Postives = 289/495 (58.38%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           MAE GH FS IF LTLLH LHF S KL VSTPPPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MAEKGHLFSSIFFLTLLHILHFSSPKLEVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTG ESP +FNPT+L+AAQWAATAAAA ISLM+LTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGAESPALFNPTSLNAAQWAATAAAAGISLMVLTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSV RSPWK+G GDVVKEF                                         
Sbjct: 121 HSVVRSPWKDGRGDVVKEFVTAAAAHGVDVGLYLSPWDRHDRRYSHDLPYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLNKYGNVREIWFDGAKGQNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDVRWVGNEKG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGTPCWSTINRTLLAIGAEGISEYLNKGDPKGTHWVPPECDVSIRKGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 326
                 YYNSVGRNCVLL NVPPNSTGLIAQEDA TL QFKAAI TIF++NLA NCS++A
Sbjct: 301 NELLKIYYNSVGRNCVLLVNVPPNSTGLIAQEDADTLKQFKAAIDTIFTTNLAHNCSVKA 360

[Close]

BLAST of Carg08517-RA vs. NCBI nr
Match: XP_022970037.1 (putative alpha-L-fucosidase 1 [Cucurbita maxima])

HSP 1 Score: 482.6 bits (1241), Expect = 2.6e-132
Identity = 271/495 (54.75%), Postives = 288/495 (58.18%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           MAE GH FS IF LT LH LHF S KL VSTPPPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MAEKGHLFSSIFFLTFLHILHFSSPKLEVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTG ESP +FNPT+L+ AQWAATAAAA ISLMILTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGAESPALFNPTSLNPAQWAATAAAAGISLMILTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSV RSPWK+G GDVVKEF                                         
Sbjct: 121 HSVVRSPWKDGRGDVVKEFVSAATAHGVDVGLYLSPWDRHDRRYSHDLPYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLQKYGNVREIWFDGAKGQNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDIRWVGNEKG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGMPCWSTINRTLLAIGAEGISEYLNKGDPKGTHWVPPECDVSIRKGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 326
                 YYNSVGRNCVLL NVPPNSTGLIAQEDA TL QFKAAI TIF++NLA NCS++A
Sbjct: 301 NELLNIYYNSVGRNCVLLVNVPPNSTGLIAQEDADTLKQFKAAIDTIFTTNLAHNCSVKA 360

[Close]

BLAST of Carg08517-RA vs. ExPASy Swiss-Prot
Match: Q7XUR3 (Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0560400 PE=3 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 1.7e-54
Identity = 152/462 (32.90%), Postives = 193/462 (41.77%), Query Frame = 0


Query: 31  TPPPLPILPLPSFSQLKWQQREVIMFLHFGPNTFTDSEWGTGRESPTVFNPTALDAAQWA 90
           TPPPLP+LP+PS++QL+WQ  E+ +FLHFGPNTFTDSEWG+ R  P VF P+ALDA QWA
Sbjct: 30  TPPPLPVLPVPSYAQLQWQLSEMALFLHFGPNTFTDSEWGSVRADPAVFAPSALDAGQWA 89

Query: 91  ATAAAANISLMILTAKHHDGFCLWPSKYTTHSVSRSPWKNGHGDVVKE------------ 150
             AAA     ++LTAKHHDGFCLWPS  T +SV+ SPWK G GDVV E            
Sbjct: 90  RAAAAGGFGRVVLTAKHHDGFCLWPSALTNYSVAASPWKGGAGDVVGELAAAARAEGIGL 149

Query: 151 ------------------------------------------------------------ 210
                                                                       
Sbjct: 150 GLYLSPWDRHEPVYGDTVAYNEHYLGQMTELLTRYGDVEEVWLDGAKGEGKDMDYMFDAW 209

Query: 211 ------------------------------------------------------------ 270
                                                                       
Sbjct: 210 FALIHQLQQRVVIFSDAGPDTRWVGDEAGVAGYTCWSPFNKSTVTIGHIIPEYSRCGDPF 269

Query: 271 ---------------------------------FYYNSVGRNCVLLFNVPPNSTGLIAQE 320
                                             YY SVGRNC+L+ NVPPNS+GLI+ E
Sbjct: 270 GQDWVPAECDVSIRPGWFWHASEKPKNATTLLDIYYKSVGRNCLLILNVPPNSSGLISTE 329

[Close]

BLAST of Carg08517-RA vs. ExPASy Swiss-Prot
Match: Q8GW72 (Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2)

HSP 1 Score: 202.6 bits (514), Expect = 6.8e-51
Identity = 152/487 (31.21%), Postives = 205/487 (42.09%), Query Frame = 0


Query: 8   FSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPNTFTDS 67
           F F F++  L  +   S+ L    P P PILPLPS  QL+WQ   + MFLHFGPNTFTDS
Sbjct: 8   FFFFFSILSLSQISNSSSLL---KPHPCPILPLPSSQQLQWQLGSMAMFLHFGPNTFTDS 67

Query: 68  EWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTTHSVSRSP 127
           EWGTG+ +P++FNPT L+A+QW   A  +  S +ILTAKHHDGFCLWPS+YT +SV  S 
Sbjct: 68  EWGTGKANPSIFNPTHLNASQWVQIAKDSGFSRVILTAKHHDGFCLWPSEYTDYSVKSSQ 127

Query: 128 WKNGHGDVVKE------------------------------------------------- 187
           W+NG GDVV E                                                 
Sbjct: 128 WRNGAGDVVAELASAAKEAGIGLGLYLSPWDRHEQCYGKTLEYNEFYLSQMTELLTKYGE 187

Query: 188 ------------------------------------------------------------ 247
                                                                       
Sbjct: 188 IKEVWLDGAKGDGEKDMEYFFDTWFSLIHQLQPKAVIFSDAGPDVRWIGDEAGLAGSTCW 247

Query: 248 ---------------------------------------------------------FYY 307
                                                                     YY
Sbjct: 248 SLFNRTNAKIGDTEPSYSQEGDGYGQDWVPAECDVSIRPGWFWHASESPKPAVQLLDIYY 307

Query: 308 NSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRASSQR---V 320
           NSVGRNC+ L NVPPNS+GLI+++D   L +F    ++IFS+NLA+   + +SS R    
Sbjct: 308 NSVGRNCLFLLNVPPNSSGLISEQDIKVLEEFSEMKNSIFSNNLARKAFVNSSSIRGDQS 367

[Close]

BLAST of Carg08517-RA vs. ExPASy Swiss-Prot
Match: C3YWU0 (Alpha-L-fucosidase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_56888 PE=3 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 1.6e-04
Identity = 40/134 (29.85%), Postives = 62/134 (46.27%), Query Frame = 0


Query: 88  QWAATAAAANISLMILTAKHHDGFCLWPSKYT--THSVSRSPWKNGHGDVVKEFYYNSVG 147
           QWA    A+    ++LT+KHH+GF  WPSKY+   +SV   P    H D+V E    ++ 
Sbjct: 101 QWAEVLQASGAKYVVLTSKHHEGFTNWPSKYSWNWNSVDNGP----HRDLVGELAM-AIR 160

Query: 148 RNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRASSQRVSAFGPEN 207
            N  L F +  +       E  H L   K   +   + +  ++  L    + V+A+ PE 
Sbjct: 161 NNSDLHFGLYYS-----LFEWFHPL-YLKDKQNKWTTQDYTKDVGLAELYELVNAYHPEV 220

Query: 208 VLDNDHW---WTYW 217
           V  +  W   +TYW
Sbjct: 221 VWSDGDWEAPYTYW 223

[Close]

BLAST of Carg08517-RA vs. ExPASy Swiss-Prot
Match: Q9BTY2 (Plasma alpha-L-fucosidase OS=Homo sapiens OX=9606 GN=FUCA2 PE=1 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 4.8e-04
Identity = 31/112 (27.68%), Postives = 55/112 (49.11%), Query Frame = 0


Query: 37  ILPLPSFSQ----LKWQQREVIMFLHF----GPNTFTDSEWGTGRESPTVFNPTALDAAQ 96
           +  +PSF        WQ+ ++  ++ F     P +F   ++G       +F     +A Q
Sbjct: 63  VFSVPSFGSEWFWWYWQKEKIPKYVEFMKDNYPPSFKYEDFG------PLFTAKFFNANQ 122

Query: 97  WAATAAAANISLMILTAKHHDGFCLWPSKYT--THSVSRSPWKNGHGDVVKE 139
           WA    A+    ++LT+KHH+GF LW S+Y+   +++   P +    D+VKE
Sbjct: 123 WADIFQASGAKYIVLTSKHHEGFTLWGSEYSWNWNAIDEGPKR----DIVKE 164

[Close]

BLAST of Carg08517-RA vs. ExPASy Swiss-Prot
Match: Q9VTJ4 (Putative alpha-L-fucosidase OS=Drosophila melanogaster OX=7227 GN=Fuca PE=2 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 6.3e-04
Identity = 26/70 (37.14%), Postives = 38/70 (54.29%), Query Frame = 0


Query: 71  TGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSK--YTTHSVSRSPW 130
           T +E  + F     +A +WA     +    ++LT+KHHDGF LWPSK  Y  +S+   P 
Sbjct: 99  TYQEFASQFTAELFNATKWALLFKDSGARYVVLTSKHHDGFTLWPSKNSYGWNSMDVGPK 158

Query: 131 KNGHGDVVKE 139
           +    D+VKE
Sbjct: 159 R----DIVKE 164

[Close]

BLAST of Carg08517-RA vs. ExPASy TrEMBL
Match: A0A6J1H387 (Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111459601 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.7e-161
Identity = 317/496 (63.91%), Postives = 318/496 (64.11%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSVSRSPWKNGHGDVVKEF                                         
Sbjct: 121 HSVSRSPWKNGHGDVVKEFVDAAAPRGIDVGVYLSPWDRHDRRYSHELAYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLNNYGNVREIWFDGAKGKNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDIRWVGDENG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGTPCWSTINRSSLAIGAEGITQYLNEGDPEGTHWVPPECDVSIRNGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 327
                 YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA
Sbjct: 301 KTLLEIYYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 360

[Close]

BLAST of Carg08517-RA vs. ExPASy TrEMBL
Match: A0A6J1ELW0 (Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111435799 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 5.7e-133
Identity = 272/495 (54.95%), Postives = 289/495 (58.38%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           MAE GH FS IF LTLLH LHF S KL VSTPPPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MAEKGHLFSSIFFLTLLHILHFSSPKLEVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTG ESP +FNPT+L+AAQWAATAAAA ISLMILTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGAESPALFNPTSLNAAQWAATAAAAGISLMILTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSV RSPWK+G GDVVKEF                                         
Sbjct: 121 HSVVRSPWKDGRGDVVKEFVTAAAAHGVDVGLYLSPWDRHDRRYSHDLPYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLNKYGNVREIWFDGAKGQNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDVRWVGNEKG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGTPCWSTINRTLLAIGAEGISEYLNKGDPKGTHWVPPECDVSIRKGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 326
                 YYNSVGRNCVLL NVPPNSTGLIAQEDA TL QFKAAI TIF++NLA NCS++A
Sbjct: 301 NELLKIYYNSVGRNCVLLVNVPPNSTGLIAQEDADTLKQFKAAIDTIFTTNLAHNCSVKA 360

[Close]

BLAST of Carg08517-RA vs. ExPASy TrEMBL
Match: A0A6J1HY04 (Alpha-L-fucosidase OS=Cucurbita maxima OX=3661 GN=LOC111469046 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.3e-132
Identity = 271/495 (54.75%), Postives = 288/495 (58.18%), Query Frame = 0


Query: 1   MAEMGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60
           MAE GH FS IF LT LH LHF S KL VSTPPPLPILPLPSFSQLKWQQREVIMFLHFG
Sbjct: 1   MAEKGHLFSSIFFLTFLHILHFSSPKLEVSTPPPLPILPLPSFSQLKWQQREVIMFLHFG 60

Query: 61  PNTFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTT 120
           PNTFTDSEWGTG ESP +FNPT+L+ AQWAATAAAA ISLMILTAKHHDGFCLWPSKYTT
Sbjct: 61  PNTFTDSEWGTGAESPALFNPTSLNPAQWAATAAAAGISLMILTAKHHDGFCLWPSKYTT 120

Query: 121 HSVSRSPWKNGHGDVVKEF----------------------------------------- 180
           HSV RSPWK+G GDVVKEF                                         
Sbjct: 121 HSVVRSPWKDGRGDVVKEFVSAATAHGVDVGLYLSPWDRHDRRYSHDLPYNEYYLAQLQE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLQKYGNVREIWFDGAKGQNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDIRWVGNEKG 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FAGMPCWSTINRTLLAIGAEGISEYLNKGDPKGTHWVPPECDVSIRKGWFWHKSESPKSL 300

Query: 301 ------YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRA 326
                 YYNSVGRNCVLL NVPPNSTGLIAQEDA TL QFKAAI TIF++NLA NCS++A
Sbjct: 301 NELLNIYYNSVGRNCVLLVNVPPNSTGLIAQEDADTLKQFKAAIDTIFTTNLAHNCSVKA 360

[Close]

BLAST of Carg08517-RA vs. ExPASy TrEMBL
Match: A0A1S3ATS4 (Alpha-L-fucosidase OS=Cucumis melo OX=3656 GN=LOC103482884 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 4.5e-122
Identity = 255/494 (51.62%), Postives = 275/494 (55.67%), Query Frame = 0


Query: 4   MGHRFSFIFTLTLLHFLHFCSAK-LGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPN 63
           MGH FS IF LT LH LHF S K L VSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPN
Sbjct: 6   MGHHFSSIFFLTFLHLLHFSSPKLLEVSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPN 65

Query: 64  TFTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTTHS 123
           TFTDSEWGTG ESP +FNPTALDA QWAATAAAA ISLMILTAKHHDGFCLWPSKYTTHS
Sbjct: 66  TFTDSEWGTGAESPAIFNPTALDATQWAATAAAAGISLMILTAKHHDGFCLWPSKYTTHS 125

Query: 124 VSRSPWKNGHGDVVKEF------------------------------------------- 183
           VSRSPWKNG GDVV+EF                                           
Sbjct: 126 VSRSPWKNGQGDVVEEFVTAATAQRIDVGLYLSPWDRHDRRYSHDLPYNEYYLAQLQELL 185

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 186 KKYGNVKEIWFDGAKGQNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDVRWVGNEKGFA 245

Query: 244 ------------------------------------------------------------ 303
                                                                       
Sbjct: 246 GTSCWSTINRTSLAIGAEGITQYLNKGDPKGRDWVPPECDVSIREGWFWHKTQSPKTLKH 305

Query: 304 ----YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRASS 327
               YYNSVGRNCVLL NVPPNSTGLI Q+DA  L QFK  I  IF++NLAQNCS++ASS
Sbjct: 306 LLKIYYNSVGRNCVLLINVPPNSTGLIPQKDAQILKQFKNTIDRIFTTNLAQNCSIKASS 365

[Close]

BLAST of Carg08517-RA vs. ExPASy TrEMBL
Match: A0A0A0KK59 (Alpha-L-fucosidase OS=Cucumis sativus OX=3659 GN=Csa_5G146320 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.9e-121
Identity = 251/493 (50.91%), Postives = 273/493 (55.38%), Query Frame = 0


Query: 4   MGHRFSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPNT 63
           MGH  S IF LT LH LHF S KL VSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPNT
Sbjct: 5   MGHHCSSIFFLTFLHLLHFSSPKLEVSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPNT 64

Query: 64  FTDSEWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTTHSV 123
           FTDSEWGTG ESP +FNPTAL+A QWAATAAAA ISLMILTAKHHDGFCLWPSKYTTHSV
Sbjct: 65  FTDSEWGTGAESPAIFNPTALNATQWAATAAAAGISLMILTAKHHDGFCLWPSKYTTHSV 124

Query: 124 SRSPWKNGHGDVVKEF-------------------------------------------- 183
           SRSPWKNG GDVV+EF                                            
Sbjct: 125 SRSPWKNGQGDVVEEFVTAATAHGIDVGLYLSPWDRHDRRYSHDLPYNEYYLAQLQELLK 184

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 185 RYGNVKEIWFDGAKGQNAPNMSYYFSDWFAMVKELQSSINIFSDAGPDVRWVGNEKGFAG 244

Query: 244 ------------------------------------------------------------ 303
                                                                       
Sbjct: 245 TSCWSTINRTSLAIGAEGITPYLNKGDPRGRDWVPPECDVSIREGWFWHKTQSPKTLEHL 304

Query: 304 ---YYNSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRASSQ 327
              YYNSVGRNCVLL NVPPNSTGLI Q+DA  L QFK  I  IF++NLAQNCS++ASSQ
Sbjct: 305 LKIYYNSVGRNCVLLINVPPNSTGLIPQKDAQVLKQFKNTIDRIFTTNLAQNCSIKASSQ 364

[Close]

BLAST of Carg08517-RA vs. TAIR 10
Match: AT2G28100.1 (alpha-L-fucosidase 1 )

HSP 1 Score: 202.6 bits (514), Expect = 4.8e-52
Identity = 152/487 (31.21%), Postives = 205/487 (42.09%), Query Frame = 0


Query: 8   FSFIFTLTLLHFLHFCSAKLGVSTPPPLPILPLPSFSQLKWQQREVIMFLHFGPNTFTDS 67
           F F F++  L  +   S+ L    P P PILPLPS  QL+WQ   + MFLHFGPNTFTDS
Sbjct: 8   FFFFFSILSLSQISNSSSLL---KPHPCPILPLPSSQQLQWQLGSMAMFLHFGPNTFTDS 67

Query: 68  EWGTGRESPTVFNPTALDAAQWAATAAAANISLMILTAKHHDGFCLWPSKYTTHSVSRSP 127
           EWGTG+ +P++FNPT L+A+QW   A  +  S +ILTAKHHDGFCLWPS+YT +SV  S 
Sbjct: 68  EWGTGKANPSIFNPTHLNASQWVQIAKDSGFSRVILTAKHHDGFCLWPSEYTDYSVKSSQ 127

Query: 128 WKNGHGDVVKE------------------------------------------------- 187
           W+NG GDVV E                                                 
Sbjct: 128 WRNGAGDVVAELASAAKEAGIGLGLYLSPWDRHEQCYGKTLEYNEFYLSQMTELLTKYGE 187

Query: 188 ------------------------------------------------------------ 247
                                                                       
Sbjct: 188 IKEVWLDGAKGDGEKDMEYFFDTWFSLIHQLQPKAVIFSDAGPDVRWIGDEAGLAGSTCW 247

Query: 248 ---------------------------------------------------------FYY 307
                                                                     YY
Sbjct: 248 SLFNRTNAKIGDTEPSYSQEGDGYGQDWVPAECDVSIRPGWFWHASESPKPAVQLLDIYY 307

Query: 308 NSVGRNCVLLFNVPPNSTGLIAQEDAHTLTQFKAAIHTIFSSNLAQNCSLRASSQR---V 320
           NSVGRNC+ L NVPPNS+GLI+++D   L +F    ++IFS+NLA+   + +SS R    
Sbjct: 308 NSVGRNCLFLLNVPPNSSGLISEQDIKVLEEFSEMKNSIFSNNLARKAFVNSSSIRGDQS 367

The following BLAST results are available for this feature:

BLAST of Carg08517-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958355.1	3.5e-161	63.91	putative alpha-L-fucosidase 1 [Cucurbita moschata]	[more]
XP_023534414.1	1.5e-159	63.25	putative alpha-L-fucosidase 1 [Cucurbita pepo subsp. pepo]	[more]
XP_022929122.1	1.2e-132	54.95	alpha-L-fucosidase 1-like [Cucurbita moschata]	[more]
KAG6579606.1	1.5e-132	54.75	Alpha-L-fucosidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022970037.1	2.6e-132	54.75	putative alpha-L-fucosidase 1 [Cucurbita maxima]	[more]

BLAST of Carg08517-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XUR3	1.7e-54	32.90	Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0...	[more]
Q8GW72	6.8e-51	31.21	Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2	[more]
C3YWU0	1.6e-04	29.85	Alpha-L-fucosidase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_56888 PE=3 SV...	[more]
Q9BTY2	4.8e-04	27.68	Plasma alpha-L-fucosidase OS=Homo sapiens OX=9606 GN=FUCA2 PE=1 SV=2	[more]
Q9VTJ4	6.3e-04	37.14	Putative alpha-L-fucosidase OS=Drosophila melanogaster OX=7227 GN=Fuca PE=2 SV=2	[more]

BLAST of Carg08517-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H387	1.7e-161	63.91	Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111459601 PE=3 SV=1	[more]
A0A6J1ELW0	5.7e-133	54.95	Alpha-L-fucosidase OS=Cucurbita moschata OX=3662 GN=LOC111435799 PE=3 SV=1	[more]
A0A6J1HY04	1.3e-132	54.75	Alpha-L-fucosidase OS=Cucurbita maxima OX=3661 GN=LOC111469046 PE=3 SV=1	[more]
A0A1S3ATS4	4.5e-122	51.62	Alpha-L-fucosidase OS=Cucumis melo OX=3656 GN=LOC103482884 PE=3 SV=1	[more]
A0A0A0KK59	2.9e-121	50.91	Alpha-L-fucosidase OS=Cucumis sativus OX=3659 GN=Csa_5G146320 PE=3 SV=1	[more]

BLAST of Carg08517-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28100.1	4.8e-52	31.21	alpha-L-fucosidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000933	Glycoside hydrolase, family 29	SMART	SM00812	alpha_l_fucos	coord: 38..201 e-value: 0.0038 score: -138.6
IPR000933	Glycoside hydrolase, family 29	PFAM	PF01120	Alpha_L_fucos	coord: 77..139 e-value: 2.2E-11 score: 43.8
IPR000933	Glycoside hydrolase, family 29	PANTHER	PTHR10030	ALPHA-L-FUCOSIDASE	coord: 138..322 coord: 17..139
None	No IPR available	GENE3D	2.60.120.260		coord: 192..315 e-value: 9.0E-18 score: 66.2
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 36..139 e-value: 1.0E-45 score: 158.5 coord: 140..187 e-value: 1.2E-8 score: 36.4
None	No IPR available	PANTHER	PTHR10030:SF37	OS09G0520800 PROTEIN	coord: 138..322 coord: 17..139
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	49785	Galactose-binding domain-like	coord: 181..267
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 34..139

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg08517	Carg08517	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08517-RA:2	Carg08517-RA:2	exon
Carg08517-RA:1	Carg08517-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08517-RA:cds	Carg08517-RA:cds	CDS
Carg08517-RA:cds	Carg08517-RA:cds_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg08517-RA	Carg08517-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004560	alpha-L-fucosidase activity