Carg08355-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg08355-RA vs. NCBI nr
Match: KAG7029790.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1258.0 bits (3254), Expect = 0.0e+00
Identity = 632/632 (100.00%), Postives = 632/632 (100.00%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGYLNNKEATEKTIDKNGWLHTGDLGFIDDDDEIF 420
           CGTVVRNAEMKIIHPETGVSLPRNHPGYLNNKEATEKTIDKNGWLHTGDLGFIDDDDEIF
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGYLNNKEATEKTIDKNGWLHTGDLGFIDDDDEIF 420

Query: 421 IVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSKGSKI 480
           IVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSKGSKI
Sbjct: 421 IVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSKGSKI 480

Query: 481 SEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGTPLRV 540
           SEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGTPLRV
Sbjct: 481 SEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGTPLRV 540

Query: 541 HPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKDRHQI 600
           HPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKDRHQI
Sbjct: 541 HPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKDRHQI 600

Query: 601 QLPSGPSVLIGTLSCVYPPSGNNEKAGTSSGV 633
           QLPSGPSVLIGTLSCVYPPSGNNEKAGTSSGV
Sbjct: 601 QLPSGPSVLIGTLSCVYPPSGNNEKAGTSSGV 632

BLAST of Carg08355-RA vs. NCBI nr
Match: XP_022929776.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 983.0 bits (2540), Expect = 1.2e-282
Identity = 519/607 (85.50%), Postives = 519/607 (85.50%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVRSKGSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQ------------------------------------ 531

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 596
                                                   VIYYKKIRKVFFVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVFFVDSIPVAP 531

BLAST of Carg08355-RA vs. NCBI nr
Match: XP_022997404.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 979.5 bits (2531), Expect = 1.3e-281
Identity = 516/607 (85.01%), Postives = 519/607 (85.50%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LA+RRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LASRRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFA+ENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAMENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMS+LLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSTLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVRSKGSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQ------------------------------------ 531

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 596
                                                   VIYYKKIRKVFFVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVFFVDSIPVAP 531

BLAST of Carg08355-RA vs. NCBI nr
Match: XP_023546819.1 (4-coumarate--CoA ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976.9 bits (2524), Expect = 8.5e-281
Identity = 518/617 (83.95%), Postives = 523/617 (84.76%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCF+HLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFQHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFA+ENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFALENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVR++GSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRTEGSKISEDDIKKYISDQ------------------------------------ 540

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 600
                                                   VIYYKKIRKV FVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVLFVDSIPVAP 540

Query: 601 SGKILRKDRHQIQLPSG 606
           SGKILRK   + QL SG
Sbjct: 601 SGKILRKVL-KAQLESG 540

BLAST of Carg08355-RA vs. NCBI nr
Match: XP_038890953.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 910.2 bits (2351), Expect = 9.8e-261
Identity = 475/607 (78.25%), Postives = 500/607 (82.37%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD  L N QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN ATG++HTYAAVD
Sbjct: 1   MDHGLMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGATGEVHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARRVA GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAISTTANPLYKP EI KQ
Sbjct: 61  LAARRVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AA ARPKVIITQAEFVEKV  +AVE+GVKI+CTDSPPVGCLRFSE++EADEN+IPAVKIN
Sbjct: 121 AAGARPKVIITQAEFVEKVWGYAVEHGVKILCTDSPPVGCLRFSEVMEADENDIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV+L SNDV+IC+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLYSNDVIICVLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILIL+KYDMSSLL LIQT+KATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILKKYDMSSLLPLIQTYKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGK LEDTVKARLPNATLGQGYGMTEAGPVLSMCL FAKEPFE KSGA
Sbjct: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKII+PET VSLPRNHP            GY+NN+EATEKTIDK+GWLHTG
Sbjct: 361 CGTVVRNAEMKIINPETSVSLPRNHPGEICIRGSQIMKGYINNREATEKTIDKDGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDD+DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN+SSAAV+PMEDEAAGEIP
Sbjct: 421 DLGFIDDNDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNISSAAVIPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
            AFVVRSKGSKISEDD+KKYISDQ                                    
Sbjct: 481 VAFVVRSKGSKISEDDLKKYISDQ------------------------------------ 531

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 596
                                                   VIYYKKIRKVFFVDSIP+AP
Sbjct: 541 ----------------------------------------VIYYKKIRKVFFVDSIPIAP 531

BLAST of Carg08355-RA vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 1.2e-208
Identity = 375/609 (61.58%), Postives = 448/609 (73.56%), Query Frame = 0

Query: 2   DSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDL 61
           + Q  + ++FIFRSKLPDIYIPNHLPLH+YCFE++SQF  RPCLIN ATG+I TYA VDL
Sbjct: 4   NKQDDHQEEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDL 63

Query: 62  AARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQA 121
            +R+VA GL K+G+ QGDVIMLLLQNSPEFV+AFL ASY GAI TTANP Y PAE+ KQA
Sbjct: 64  TSRKVAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQA 123

Query: 122 ATARPKVIITQAEFVEKVREF-AVENGVKIICTDSPP--VGCLRFSEILEADENEIPAVK 181
           A ++ K++IT A +++KV+EF   E+GVK++C D+PP    CL FSE+ +ADE EIPAVK
Sbjct: 124 AASKTKLVITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVK 183

Query: 182 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFH 241
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDG+NPN+  + NDV++C+LPLFH
Sbjct: 184 IHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFH 243

Query: 242 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 301
           IYSLNS+LLCGLRVGAAILI+QK+++S LL LI+  K TIAPFVPPIVL  AK PD+HRY
Sbjct: 244 IYSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRY 303

Query: 302 DLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKS 361
           DLSSIR VMSG APMGK LED VK +LP+A LGQGYGMTEAGPVLSMCL FAKEPF  KS
Sbjct: 304 DLSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKS 363

Query: 362 GACGTVVRNAEMKIIHPETGVSLPRNH------------PGYLNNKEATEKTIDKNGWLH 421
           GACGTVVRNAEMKI+ P+TG SLPRN              GY+N+ EAT+ TID+ GWLH
Sbjct: 364 GACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLH 423

Query: 422 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGE 481
           TGD+GFID+DDE+FIVDRLKELIKYKG+QVAPAELE++L S+PN++ AAVVPM+DEAAGE
Sbjct: 424 TGDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGE 483

Query: 482 IPAAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHP 541
           +P AFVVRS GSKI+E+DIK+YIS Q                                  
Sbjct: 484 VPVAFVVRSNGSKITEEDIKQYISKQ---------------------------------- 536

Query: 542 FSGSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPV 596
                                                     V++YK+I K FF++ IP 
Sbjct: 544 ------------------------------------------VVFYKRINKAFFIEEIPK 536

BLAST of Carg08355-RA vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 2.1e-205
Identity = 363/608 (59.70%), Postives = 444/608 (73.03%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD +   + D IFRSKLPDIYIP HLPLH+YCFE+LS+F+ RPCLI+ A  +I+TYA V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           L +R+VA GL+K+G+ Q D IM+LL N PEFVFAF+GASY GAIST ANPL+ PAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           A  +  K++ITQA F  KV+++A+EN +K+IC DS P GC+ FSE++++DE+EIP VKI 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
            +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGEN N+ ++S+DVL+C+LPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +H YDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SS+R VMSGAAP+GK LED V+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPF+ KSGA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKI+ P+TG SLPRN P            GYLN+ EAT +TI+K GWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           D+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P++S AAVVPM DE AGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
            AFVVRS GS I+ED++K +IS Q                                    
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQ------------------------------------ 534

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 597
                                                   VI+YK+I++VFFV+++P +P
Sbjct: 543 ----------------------------------------VIFYKRIKRVFFVETVPKSP 534

BLAST of Carg08355-RA vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 1.4e-204
Identity = 362/608 (59.54%), Postives = 444/608 (73.03%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD++   + D IFRSKLPDIYIP HLPLH+YCFE+LS+F+ RPCLI+ A  +I+TYA V+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           L +R+VA GL+K+G+ Q D IM+LL N PEFVFAF+GASY GAIST ANPL+ PAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           A  +  K++ITQA F  KV+++A+EN +K+IC DS P GC+ FSE++++DE+EIP VKI 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
            +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGEN N+ ++S+DVL+C+LPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +  YDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SS+R VMSGAAP+GK LED V+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPF+ KSGA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKI+ P+TG SLPRN P            GYLN+ EAT +TI+K GWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           D+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P++S AAVVPM DE AGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
            AFVVRS GS I+ED++K +IS Q                                    
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQ------------------------------------ 534

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 597
                                                   VI+YK+I++VFFV+++P +P
Sbjct: 543 ----------------------------------------VIFYKRIKRVFFVETVPKSP 534

BLAST of Carg08355-RA vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.3e-204
Identity = 362/599 (60.43%), Postives = 438/599 (73.12%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVATG 69
           D IFRSKLPDIYIP HLPLH+YCFE++S+F  RPCLIN A  QI+TYA V+L  R+VA G
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVG 73

Query: 70  LSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPKVI 129
           L+K+G+ Q D IM+LL NSPEFVFAF+GASY GAIST ANPL+ PAE+ KQA  +  K+I
Sbjct: 74  LNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKII 133

Query: 130 ITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKINSNDVVALPF 189
           ITQ+ FV KV+++A EN VK+IC DS P GCL FSE+ ++DE+EIP VKI  +DVVALP+
Sbjct: 134 ITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDDVVALPY 193

Query: 190 SSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLNSVLLCG 249
           SSGTTG+PKGVMLTHK LVTS+AQQVDGEN N+ ++S DVL+C+LPLFHIYSLNS+LLCG
Sbjct: 194 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILLCG 253

Query: 250 LRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMSG 309
           LRVGAAILI+QK+D++  L LIQ +K +I PFVPPIVL  AK+P +  YDLSS+R VMSG
Sbjct: 254 LRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVMSG 313

Query: 310 AAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGTVVRNAE 369
           AAP+GK LED V+ + PNA LGQGYGMTEAGPVL+MCL FAKEPF+ KSGACGTVVRNAE
Sbjct: 314 AAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAE 373

Query: 370 MKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTGDLGFIDDDD 429
           MKI+ P+TG SLPRN P            GYLN+ EAT +TIDK GWLHTGD+GFID+DD
Sbjct: 374 MKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDD 433

Query: 430 EIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSKG 489
           E+FIVDRLKELIKYKG+QVAPAE+EALL ++PN+S AAVVPM+DE AGE+P AFVVRS G
Sbjct: 434 ELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 493

Query: 490 SKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGTP 549
           S I+ED++K +IS Q                                             
Sbjct: 494 SAITEDEVKDFISKQ--------------------------------------------- 536

Query: 550 LRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKD 597
                                          VI+YK++++VFFV+++P +PSGKILRKD
Sbjct: 554 -------------------------------VIFYKRVKRVFFVETVPKSPSGKILRKD 536

BLAST of Carg08355-RA vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.5e-203
Identity = 368/603 (61.03%), Postives = 438/603 (72.64%), Query Frame = 0

Query: 6   TNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARR 65
           T   D IFRSKLPDIYIPNHLPLH+YCFE++S+F  RPCLIN A  QI+TYA V+L +R+
Sbjct: 5   TKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRK 64

Query: 66  VATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATAR 125
           VA GL K G+   D IM+LL NSPEFVFAF+GASY GAIST ANPL+ PAE+ KQA  + 
Sbjct: 65  VAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASS 124

Query: 126 PKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKINSNDVV 185
            K+I+TQA  V KV+++A EN VKIIC DS P GCL FS + +A+E++IP V+I  +DVV
Sbjct: 125 AKIIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVV 184

Query: 186 ALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLNSV 245
           ALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+ ++S DV++C+LPLFHIYSLNSV
Sbjct: 185 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSV 244

Query: 246 LLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRI 305
           LLCGLRVGAAILI+QK+D+ S L LIQ +K TI PFVPPIVL  AK+P +  YDLSS+R 
Sbjct: 245 LLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRT 304

Query: 306 VMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGTVV 365
           VMSGAAP+GK LEDTV+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPFE KSGACGTVV
Sbjct: 305 VMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVV 364

Query: 366 RNAEMKIIHPETGVSLPRNH------------PGYLNNKEATEKTIDKNGWLHTGDLGFI 425
           RNAEMKI+ P+TG SLPRN              GYLN+ EAT +TIDK GWL+TGD+G+I
Sbjct: 365 RNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYI 424

Query: 426 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVV 485
           DDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++PN+S AAVVPM+DE AGE+P AFVV
Sbjct: 425 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVV 484

Query: 486 RSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSIL 545
           RS GS I+ED++K +IS Q                                         
Sbjct: 485 RSNGSTITEDEVKDFISKQ----------------------------------------- 531

Query: 546 AGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKIL 597
                                              VI+YK+I++VFFVD+IP +PSGKIL
Sbjct: 545 -----------------------------------VIFYKRIKRVFFVDAIPKSPSGKIL 531

BLAST of Carg08355-RA vs. ExPASy TrEMBL
Match: A0A6J1EP35 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436262 PE=4 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 5.8e-283
Identity = 519/607 (85.50%), Postives = 519/607 (85.50%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVRSKGSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQ------------------------------------ 531

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 596
                                                   VIYYKKIRKVFFVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVFFVDSIPVAP 531

BLAST of Carg08355-RA vs. ExPASy TrEMBL
Match: A0A6J1KB99 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111492331 PE=4 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 6.4e-282
Identity = 516/607 (85.01%), Postives = 519/607 (85.50%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LA+RRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LASRRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFA+ENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAMENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMS+LLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSTLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVRSKGSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQ------------------------------------ 531

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 596
                                                   VIYYKKIRKVFFVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVFFVDSIPVAP 531

BLAST of Carg08355-RA vs. ExPASy TrEMBL
Match: A0A5D3CKT4 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00190 PE=4 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 1.1e-254
Identity = 460/599 (76.79%), Postives = 489/599 (81.64%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD +  N QDFIF+SKLPDIYIPNHLPLHTYCFEHL+QF HRPCLIN  TG+IHTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARR A GLSKIG+GQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPA 180
           A  AR KVIITQ EFVEKV EFAVENGVKI+CTD    S   GCLRFSE++EADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++NS DV+IC+LPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEF 360
            Y+LSSIRIVMSGAAPMGK LEDTVKARLPNA LGQGYGMTEAGPVLSMCLGFAKEPF  
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPETGVSLPRNHPGYLNNKEATEKTIDKNGWLHTGDLGFIDDD 420
           KSGACGTVVRNAEMKIIHP++ +SLPRNHPGYLNN+EATEKTIDK+GWLHTGDLGFIDDD
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGYLNNEEATEKTIDKDGWLHTGDLGFIDDD 420

Query: 421 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSK 480
           DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAAGEIP AFVVRSK
Sbjct: 421 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVVRSK 480

Query: 481 GSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGT 540
           GSKISE+DIK YISDQ                                            
Sbjct: 481 GSKISENDIKNYISDQ-------------------------------------------- 523

Query: 541 PLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRK 596
                                           VIYYK+IRKVFFVDSIP+APSGKILRK
Sbjct: 541 --------------------------------VIYYKRIRKVFFVDSIPMAPSGKILRK 523

BLAST of Carg08355-RA vs. ExPASy TrEMBL
Match: A0A5A7U0C7 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1468G00210 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 8.1e-253
Identity = 460/604 (76.16%), Postives = 489/604 (80.96%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD +  N QDFIF+SKLPDIYIPNHLPLHTYCFEHL+QF HRPCLIN  TG+IHTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARR A GLSKIG+GQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPA 180
           A  AR KVIITQ EFVEKV EFAVENGVKI+CTD    S   GCLRFSE++EADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++NS DV+IC+LPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEF 360
            Y+LSSIRIVMSGAAPMGK LEDTVKARLPNA LGQGYGMTEAGPVLSMCLGFAKEPF  
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPETGVSLPRNHPG-----YLNNKEATEKTIDKNGWLHTGDLG 420
           KSGACGTVVRNAEMKIIHP++ +SLPRNHPG     YLNN+EATEKTIDK+GWLHTGDLG
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRYLNNEEATEKTIDKDGWLHTGDLG 420

Query: 421 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAF 480
           FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAAGEIP AF
Sbjct: 421 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAF 480

Query: 481 VVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSS 540
           VVRSKGSKISE+DIK YISDQ                                       
Sbjct: 481 VVRSKGSKISENDIKNYISDQ--------------------------------------- 528

Query: 541 ILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGK 596
                                                VIYYK+IRKVFFVDSIP+APSGK
Sbjct: 541 -------------------------------------VIYYKRIRKVFFVDSIPMAPSGK 528

BLAST of Carg08355-RA vs. ExPASy TrEMBL
Match: A0A1S3BSW8 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 5.3e-252
Identity = 460/611 (75.29%), Postives = 489/611 (80.03%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD +  N QDFIF+SKLPDIYIPNHLPLHTYCFEHL+QF HRPCLIN  TG+IHTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARR A GLSKIG+GQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPA 180
           A  AR KVIITQ EFVEKV EFAVENGVKI+CTD    S   GCLRFSE++EADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++NS DV+IC+LPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEF 360
            Y+LSSIRIVMSGAAPMGK LEDTVKARLPNA LGQGYGMTEAGPVLSMCLGFAKEPF  
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGW 420
           KSGACGTVVRNAEMKIIHP++ +SLPRNHP            GYLNN+EATEKTIDK+GW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPAAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPI 540
           GEIP AFVVRSKGSKISE+DIK YISDQ                                
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQ-------------------------------- 535

Query: 541 HPFSGSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSI 596
                                                       VIYYK+IRKVFFVDSI
Sbjct: 541 --------------------------------------------VIYYKRIRKVFFVDSI 535

BLAST of Carg08355-RA vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 641.3 bits (1653), Expect = 7.9e-184
Identity = 333/610 (54.59%), Postives = 420/610 (68.85%), Query Frame = 0

Query: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66
           N  D IFRSKLPDIYIPNHL LH Y F+++S+F  +PCLIN  TG ++TY+ V + +R++
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126
           A    K+GV Q DV+MLLL N PEFV +FL AS+ GA +T ANP + PAEI KQA  +  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQAEFVEKVREFAVENGVKIICTDSP-----PVGCLRFSEILEAD---ENEIPAVK 186
           K+IIT+A +V+K++    ++GV I+C D       P GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+  +S+DV++C+LP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 306
           IY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +  +Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 DLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKS 366
           DLSSIR+V SGAAP+GK LED V A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF  KS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLH 426
           GACGTVVRNAEMKI+ P+TG SL RN P            GYLNN  AT +TIDK+GWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGE 486
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+++  AVV M++EAAGE
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 498

Query: 487 IPAAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHP 546
           +P AFVV+SK S++SEDD+K+++S Q                                  
Sbjct: 499 VPVAFVVKSKDSELSEDDVKQFVSKQ---------------------------------- 552

Query: 547 FSGSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPV 597
                                                     V++YK+I KVFF +SIP 
Sbjct: 559 ------------------------------------------VVFYKRINKVFFTESIPK 552

BLAST of Carg08355-RA vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 637.9 bits (1644), Expect = 8.7e-183
Identity = 333/605 (55.04%), Postives = 418/605 (69.09%), Query Frame = 0

Query: 8   AQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVA 67
           + D IFRS+LPDIYIPNHLPLH Y FE++S+F  +PCLIN  TG+++TYA V + +R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  TGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPK 127
            GL  +GV Q DV+M+LL NSPE V  FL AS+ GAI+T+ANP + PAEI KQA  +  K
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 VIITQAEFVEKVREFAVENGVKIICTDSP--PVGCLRFSEILEADENEIPAV--KINSND 187
           +I+TQ+ +V+K++     +GV I+ TDS   P  CLRFSE+ +++E  + ++  KI+  D
Sbjct: 138 LIVTQSRYVDKIKNLQ-NDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPED 197

Query: 188 VVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLN 247
           VVALPFSSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+  N +DV++C+LP+FHIY+LN
Sbjct: 198 VVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALN 257

Query: 248 SVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSI 307
           S++LC LRVGA ILI+ K++++ LL  IQ  K T+A  VPPIVL  AK+P+  +YDLSS+
Sbjct: 258 SIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSV 317

Query: 308 RIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGT 367
           R+V SGAAP+GK LED + A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF  KSGACGT
Sbjct: 318 RMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGT 377

Query: 368 VVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTGDLG 427
           VVRNAEMKI+ P+TG SLPRN P            GYLN+  AT  TIDK+GWLHTGD+G
Sbjct: 378 VVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVG 437

Query: 428 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAF 487
           FIDDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P ++  AVV M++E AGE+P AF
Sbjct: 438 FIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAF 497

Query: 488 VVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSS 547
           VVRSK S ISED+IK+++S Q                                       
Sbjct: 498 VVRSKDSNISEDEIKQFVSKQ--------------------------------------- 545

Query: 548 ILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGK 597
                                                V++YK+I KVFF DSIP APSGK
Sbjct: 558 -------------------------------------VVFYKRINKVFFTDSIPKAPSGK 545

BLAST of Carg08355-RA vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 630.6 bits (1625), Expect = 1.4e-180
Identity = 312/506 (61.66%), Postives = 395/506 (78.06%), Query Frame = 0

Query: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66
           N  D IFRSKLPDIYIPNHL LH Y F+++S+F  +PCLIN  TG ++TY+ V + +R++
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126
           A    K+GV Q DV+MLLL N PEFV +FL AS+ GA +T ANP + PAEI KQA  +  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQAEFVEKVREFAVENGVKIICTDSP-----PVGCLRFSEILEAD---ENEIPAVK 186
           K+IIT+A +V+K++    ++GV I+C D       P GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+  +S+DV++C+LP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 306
           IY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +  +Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 DLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKS 366
           DLSSIR+V SGAAP+GK LED V A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF  KS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLH 426
           GACGTVVRNAEMKI+ P+TG SL RN P            GYLNN  AT +TIDK+GWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGE 486
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+++  AVV M++EAAGE
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 498

Query: 487 IPAAFVVRSKGSKISEDDIKKYISDQ 493
           +P AFVV+SK S++SEDD+K+++S Q
Sbjct: 499 VPVAFVVKSKDSELSEDDVKQFVSKQ 524

BLAST of Carg08355-RA vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 602.4 bits (1552), Expect = 4.1e-172
Identity = 316/600 (52.67%), Postives = 404/600 (67.33%), Query Frame = 0

Query: 12  IFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVATGLS 71
           IFRSKLPDI IPNHLPLHTYCFE LS    +PCLI  +TG+ +TY    L  RRVA+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPKVIIT 131
           K+G+ +GDVIM+LLQNS EFVF+F+GAS  GA+STTANP Y   E+ KQ  ++  K+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QAEFVEKVREFAVENGVKIICTDSP-PVGCLRFSEILEADENE--IPAVKINSNDVVALP 191
            +++V+K++       + +I TD P P  CL FS ++  DE       V I  +D  ALP
Sbjct: 154 HSQYVDKLKNLG--ENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALP 213

Query: 192 FSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLNSVLLC 251
           FSSGTTG+PKGV+LTHKSL+TS+AQQVDG+NPN+ L SNDV++C+LPLFHIYSLNSVLL 
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 252 GLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMS 311
            LR GA +L++ K+++ +LL LIQ H+ TIA  VPP+V+  AKNP ++ YDLSS+R V+S
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLS 333

Query: 312 GAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGTVVRNA 371
           GAAP+GK L+D+++ RLP A LGQGYGMTEAGPVLSM LGFAKEP   KSG+CGTVVRNA
Sbjct: 334 GAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNA 393

Query: 372 EMKIIHPETGVSLPRNHPG------------YLNNKEATEKTIDKNGWLHTGDLGFIDDD 431
           E+K++H ET +SL  N PG            YLN+ EAT  TID+ GWLHTGD+G++D+D
Sbjct: 394 ELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDED 453

Query: 432 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSK 491
           DEIFIVDRLKE+IK+KG+QV PAELE+LL ++ +++ AAVVP  DE AGE+P AFVVRS 
Sbjct: 454 DEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSN 513

Query: 492 GSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGT 551
           G+ I+E+D+K+Y++ Q                                            
Sbjct: 514 GNDITEEDVKEYVAKQ-------------------------------------------- 555

Query: 552 PLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKD 597
                                           V++YK++ KVFFV SIP +PSGKILRKD
Sbjct: 574 --------------------------------VVFYKRLHKVFFVASIPKSPSGKILRKD 555

BLAST of Carg08355-RA vs. TAIR 10
Match: AT1G51680.2 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 586.3 bits (1510), Expect = 3.0e-167
Identity = 291/471 (61.78%), Postives = 364/471 (77.28%), Query Frame = 0

Query: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66
           N  D IFRSKLPDIYIPNHL LH Y F+++S+F  +PCLIN  TG ++TY+ V + +R++
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126
           A    K+GV Q DV+MLLL N PEFV +FL AS+ GA +T ANP + PAEI KQA  +  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQAEFVEKVREFAVENGVKIICTDSP-----PVGCLRFSEILEAD---ENEIPAVK 186
           K+IIT+A +V+K++    ++GV I+C D       P GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+  +S+DV++C+LP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 306
           IY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +  +Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 DLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKS 366
           DLSSIR+V SGAAP+GK LED V A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF  KS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLH 426
           GACGTVVRNAEMKI+ P+TG SL RN P            GYLNN  AT +TIDK+GWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVV 458
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+++  AVV
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVV 489