Carg08182-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08182-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	basic blue protein-like
Location	Carg_Chr04: 2721524 .. 2722164 (+)
Sequence length	572
RNA-Seq Expression	Carg08182-RA
Synteny	Carg08182-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTCATCCATTTCCCCCACCACCACCACCTCTATTCGAGCCCTTTAGCCTAGAAATTACGAACCAATGGCTCAGGGAAGAGGCAGTGCAGCGATGGGTGCAGTGATGGTGTGCTTGTTGGTGCTCCAATTCTCAGCGATGGCTCGTGCAGCTGTTTACACCGTCGGCGGTGCTCAAGGCTGGACCTTTAATGTCGCGTCCTGGCCCAAGGGGAAGCGCTTTCGTGCTGGAGATACCCTTGGTAAGCCCGCTTAGCTTAGCTCCCCAATAATTGACACGTACTCGTTCTCTAAGGCTATGTGTTGTGCAGTGTTCAACTACAGCCCTGCGGCACACAATGTGGTGGCTGTGAGCAGAGTGGGCTACAGTGGGTGCGTGGCTCCAAGGGGCTCCAAAGTTTTTGGAACAGGAACGGATCAGATCAAGCTTGTGAAGGGACAAAACTACTTCATTTGCAGTCTCCCAGGCCACTGCCAAAGTGGAATGAAGATCGCCATTAATGCTGCTTGAATAATCGTTTAAACAACGAGTGTGAGAATGTTTTGCTTAATCTCGTGTTCGAATAAATTTACGTAATGGTTCGATCTTCGATCTTTCACCCTGTAATAGCTCAAATCTACCGTTAATGAAATTTAATATTA

mRNA sequence

TTCTCATCCATTTCCCCCACCACCACCACCTCTATTCGAGCCCTTTAGCCTAGAAATTACGAACCAATGGCTCAGGGAAGAGGCAGTGCAGCGATGGGTGCAGTGATGGTGTGCTTGTTGGTGCTCCAATTCTCAGCGATGGCTCGTGCAGCTGTTTACACCGTCGGCGGTGCTCAAGGCTGGACCTTTAATGTCGCGTCCTGGCCCAAGGGGAAGCGCTTTCGTGCTGGAGATACCCTTGTGTTCAACTACAGCCCTGCGGCACACAATGTGGTGGCTGTGAGCAGAGTGGGCTACAGTGGGTGCGTGGCTCCAAGGGGCTCCAAAGTTTTTGGAACAGGAACGGATCAGATCAAGCTTGTGAAGGGACAAAACTACTTCATTTGCAGTCTCCCAGGCCACTGCCAAAGTGGAATGAAGATCGCCATTAATGCTGCTTGAATAATCGTTTAAACAACGAGTGTGAGAATGTTTTGCTTAATCTCGTGTTCGAATAAATTTACGTAATGGTTCGATCTTCGATCTTTCACCCTGTAATAGCTCAAATCTACCGTTAATGAAATTTAATATTA

Coding sequence (CDS)

ATGGCTCAGGGAAGAGGCAGTGCAGCGATGGGTGCAGTGATGGTGTGCTTGTTGGTGCTCCAATTCTCAGCGATGGCTCGTGCAGCTGTTTACACCGTCGGCGGTGCTCAAGGCTGGACCTTTAATGTCGCGTCCTGGCCCAAGGGGAAGCGCTTTCGTGCTGGAGATACCCTTGTGTTCAACTACAGCCCTGCGGCACACAATGTGGTGGCTGTGAGCAGAGTGGGCTACAGTGGGTGCGTGGCTCCAAGGGGCTCCAAAGTTTTTGGAACAGGAACGGATCAGATCAAGCTTGTGAAGGGACAAAACTACTTCATTTGCAGTCTCCCAGGCCACTGCCAAAGTGGAATGAAGATCGCCATTAATGCTGCTTGA

Protein sequence

MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVFNYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIAINAA

Homology

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BLAST of Carg08182-RA vs. NCBI nr
Match: KAG6600348.1 (hypothetical protein SDJN03_05581, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031004.1 hypothetical protein SDJN02_05043 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 252.7 bits (644), Expect = 1.7e-63
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF
Sbjct: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA
Sbjct: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120

Query: 121 INAA 125
           INAA
Sbjct: 121 INAA 124

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BLAST of Carg08182-RA vs. NCBI nr
Match: XP_022942619.1 (basic blue protein-like [Cucurbita moschata])

HSP 1 Score: 248.4 bits (633), Expect = 3.1e-62
Identity = 122/124 (98.39%), Postives = 122/124 (98.39%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           MAQGRGSAAMGAVMVCLLVLQFSAMA AAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF
Sbjct: 1   MAQGRGSAAMGAVMVCLLVLQFSAMAHAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAVSRVGYSGCVAP GSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA
Sbjct: 61  NYSPAAHNVVAVSRVGYSGCVAPTGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120

Query: 121 INAA 125
           INAA
Sbjct: 121 INAA 124

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BLAST of Carg08182-RA vs. NCBI nr
Match: XP_023552090.1 (basic blue protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 247.3 bits (630), Expect = 7.0e-62
Identity = 121/124 (97.58%), Postives = 121/124 (97.58%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           MAQGRGSA MGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF
Sbjct: 1   MAQGRGSAVMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAVSR GYSGCVAPRGSKVFGTG DQIKLVKGQNYFICSLPGHCQSGMKIA
Sbjct: 61  NYSPAAHNVVAVSRAGYSGCVAPRGSKVFGTGKDQIKLVKGQNYFICSLPGHCQSGMKIA 120

Query: 121 INAA 125
           INAA
Sbjct: 121 INAA 124

[Close]

BLAST of Carg08182-RA vs. NCBI nr
Match: XP_022983409.1 (basic blue protein-like [Cucurbita maxima])

HSP 1 Score: 239.6 bits (610), Expect = 1.5e-59
Identity = 115/124 (92.74%), Postives = 120/124 (96.77%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           MAQGRGSA +GA++VCLLVLQFSAMARAAVYTVGGAQGW+FNVASWPKGKRFRAGDT VF
Sbjct: 1   MAQGRGSAVVGALIVCLLVLQFSAMARAAVYTVGGAQGWSFNVASWPKGKRFRAGDTFVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAVSR GYSGCVAPRGSKVFGTG DQIKLVKGQNYFICSLPGHCQSGMKIA
Sbjct: 61  NYSPAAHNVVAVSRAGYSGCVAPRGSKVFGTGKDQIKLVKGQNYFICSLPGHCQSGMKIA 120

Query: 121 INAA 125
           +NAA
Sbjct: 121 VNAA 124

[Close]

BLAST of Carg08182-RA vs. NCBI nr
Match: XP_023553060.1 (basic blue protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 220.7 bits (561), Expect = 7.0e-54
Identity = 105/124 (84.68%), Postives = 115/124 (92.74%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           M QGRGSA +GAVMVC+L+LQ+S +ARAAVYTVGGA+GWTFNVASWPKGKRFRAGDTLVF
Sbjct: 1   MGQGRGSAVVGAVMVCVLLLQYSQVARAAVYTVGGAKGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAV+RVGYS C  PRGSKVF TG DQIKLVKGQN+FIC+LPGHCQ+GMKIA
Sbjct: 61  NYSPAAHNVVAVNRVGYSRCTTPRGSKVFKTGKDQIKLVKGQNFFICTLPGHCQAGMKIA 120

Query: 121 INAA 125
           I AA
Sbjct: 121 ITAA 124

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BLAST of Carg08182-RA vs. ExPASy Swiss-Prot
Match: P00303 (Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-35
Identity = 66/95 (69.47%), Postives = 78/95 (82.11%), Query Frame = 0


Query: 29  AVYTVGGAQGWTFNVASWPKGKRFRAGDTLVFNYSPAAHNVVAVSRVGYSGCVAPRGSKV 88
           AVY VGG+ GWTFN  SWPKGKRFRAGD L+FNY+P  HNVV V++ G+S C  P G+KV
Sbjct: 1   AVYVVGGSGGWTFNTESWPKGKRFRAGDILLFNYNPXMHNVVVVNQGGFSTCNTPAGAKV 60

Query: 89  FGTGTDQIKLVKGQNYFICSLPGHCQSGMKIAINA 124
           + +G DQIKL KGQ+YFIC+ PGHCQSGMKIA+NA
Sbjct: 61  YTSGRDQIKLPKGQSYFICNFPGHCQSGMKIAVNA 95

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BLAST of Carg08182-RA vs. ExPASy Swiss-Prot
Match: P60496 (Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 8.4e-34
Identity = 72/126 (57.14%), Postives = 87/126 (69.05%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVM-VCLLVLQFSA-MARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTL 60
           MAQG GSA    V+ V L+ L F+  +A + VYTVG   GWTF  + WP GK FRAGD L
Sbjct: 1   MAQGSGSAERALVLGVVLVFLVFNCEVAESVVYTVGDGGGWTFGTSGWPAGKTFRAGDVL 60

Query: 61  VFNYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMK 120
           VF Y+PA HNVV+V   GY  C A  GS+VF +G D+I L +G NYFICS+PGHCQ G+K
Sbjct: 61  VFKYNPAVHNVVSVPAGGYKSCTASPGSRVFKSGDDRITLSRGTNYFICSVPGHCQGGLK 120

Query: 121 IAINAA 125
           IA+ AA
Sbjct: 121 IAVTAA 126

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BLAST of Carg08182-RA vs. ExPASy Swiss-Prot
Match: Q8LG89 (Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 5.1e-31
Identity = 68/129 (52.71%), Postives = 86/129 (66.67%), Query Frame = 0


Query: 1   MAQGRGSAAMGA------VMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRA 60
           MA+GRGSA+  A      + V +L+LQ +   +AA YTVG +  WTFN   WPKGK FRA
Sbjct: 1   MAKGRGSASWSARAIVTLMAVSVLLLQ-ADYVQAATYTVGDSGIWTFNAVGWPKGKHFRA 60

Query: 61  GDTLVFNYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQ 120
           GD LVFNY+P  HNVV V    Y+ C  P G+K + +G D+I L KGQN+FIC+ P HC+
Sbjct: 61  GDVLVFNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHCE 120

Query: 121 SGMKIAINA 124
           S MKIA+ A
Sbjct: 121 SDMKIAVTA 128

[Close]

BLAST of Carg08182-RA vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 2.7e-16
Identity = 40/113 (35.40%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 13  VMVCLLVLQFSAMARAAVYTVGGAQGWTF--NVASWPKGKRFRAGDTLVFNYSPAAHNVV 72
           +++ +L      +  A  +T+GG  GWT   ++ +W  G+ F  GD LVF+Y  A H+VV
Sbjct: 8   IIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPAAFHDVV 67

Query: 73  AVSRVGYSGCVAPRGSKVFGTGTDQIKL-VKGQNYFICSLPGHCQSGMKIAIN 123
            V++  +  C A +    F  G   + L   G+ YFIC +PGHC  GMK+ +N
Sbjct: 68  EVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVN 120

[Close]

BLAST of Carg08182-RA vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.8e-15
Identity = 45/119 (37.82%), Postives = 62/119 (52.10%), Query Frame = 0


Query: 8   AAMGAVMVCLL--VLQFSAMARAAVYTVGGAQGWTF--NVASWPKGKRFRAGDTLVFNYS 67
           A   A+++C L  ++  +  + A VYTVG   GW    + ++W   K F  GD+LVFNY 
Sbjct: 2   AFSNALVLCFLLAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYG 61

Query: 68  PAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVK-GQNYFICSLPGHCQSGMKIAI 122
             AH V  V    Y  C +        TG   I L K G++YFIC +PGH   GMK++I
Sbjct: 62  AGAHTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSI 120

[Close]

BLAST of Carg08182-RA vs. ExPASy TrEMBL
Match: A0A6J1FRV0 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111447602 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.5e-62
Identity = 122/124 (98.39%), Postives = 122/124 (98.39%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           MAQGRGSAAMGAVMVCLLVLQFSAMA AAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF
Sbjct: 1   MAQGRGSAAMGAVMVCLLVLQFSAMAHAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAVSRVGYSGCVAP GSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA
Sbjct: 61  NYSPAAHNVVAVSRVGYSGCVAPTGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120

Query: 121 INAA 125
           INAA
Sbjct: 121 INAA 124

[Close]

BLAST of Carg08182-RA vs. ExPASy TrEMBL
Match: A0A6J1J7A3 (basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111482017 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.1e-60
Identity = 115/124 (92.74%), Postives = 120/124 (96.77%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           MAQGRGSA +GA++VCLLVLQFSAMARAAVYTVGGAQGW+FNVASWPKGKRFRAGDT VF
Sbjct: 1   MAQGRGSAVVGALIVCLLVLQFSAMARAAVYTVGGAQGWSFNVASWPKGKRFRAGDTFVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAVSR GYSGCVAPRGSKVFGTG DQIKLVKGQNYFICSLPGHCQSGMKIA
Sbjct: 61  NYSPAAHNVVAVSRAGYSGCVAPRGSKVFGTGKDQIKLVKGQNYFICSLPGHCQSGMKIA 120

Query: 121 INAA 125
           +NAA
Sbjct: 121 VNAA 124

[Close]

BLAST of Carg08182-RA vs. ExPASy TrEMBL
Match: A0A6J1E590 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430872 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 7.6e-54
Identity = 104/124 (83.87%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           M QGRGSA +GAVMVC+L+LQ+S +ARAAVYTVGGA+GWTFNVASWPKGKRFRAGDTLVF
Sbjct: 1   MGQGRGSAVVGAVMVCMLLLQYSQVARAAVYTVGGAKGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVV V+RVGYS C  PRGSKVF TG DQIKLVKGQN+FIC+LPGHCQ+GMKIA
Sbjct: 61  NYSPAAHNVVGVNRVGYSRCTTPRGSKVFKTGKDQIKLVKGQNFFICTLPGHCQAGMKIA 120

Query: 121 INAA 125
           I AA
Sbjct: 121 ITAA 124

[Close]

BLAST of Carg08182-RA vs. ExPASy TrEMBL
Match: A0A6J1J7W1 (basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483380 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 9.9e-54
Identity = 103/124 (83.06%), Postives = 116/124 (93.55%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           M QGRGSA +GAV+VC+L+LQ+S +ARAAVYTVGGA+GWTFNVASWPKGKRFRAGDTLVF
Sbjct: 1   MGQGRGSAVVGAVVVCMLLLQYSQVARAAVYTVGGAKGWTFNVASWPKGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAV+RVGYS C+ PRGSKVF TG D+IKLVKGQN+FIC+LPGHCQ+GMKIA
Sbjct: 61  NYSPAAHNVVAVNRVGYSRCITPRGSKVFKTGKDRIKLVKGQNFFICTLPGHCQAGMKIA 120

Query: 121 INAA 125
           I AA
Sbjct: 121 ITAA 124

[Close]

BLAST of Carg08182-RA vs. ExPASy TrEMBL
Match: A0A6J1D6I4 (basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111017723 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.2e-52
Identity = 101/123 (82.11%), Postives = 112/123 (91.06%), Query Frame = 0


Query: 1   MAQGRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRAGDTLVF 60
           MA+GRGSA +GAVM CLL+LQ+S MARAAVYTVGGA GWTFN A+WP GKRFRAGDTLVF
Sbjct: 1   MAKGRGSAVVGAVMACLLLLQYSEMARAAVYTVGGAPGWTFNSAAWPNGKRFRAGDTLVF 60

Query: 61  NYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQSGMKIA 120
           NYSPAAHNVVAV++VGYS C+ PRGSKVF TG DQIKLVKGQN+FIC+LPGHCQ+GMKIA
Sbjct: 61  NYSPAAHNVVAVNKVGYSRCITPRGSKVFRTGKDQIKLVKGQNFFICNLPGHCQAGMKIA 120

Query: 121 INA 124
           I A
Sbjct: 121 ITA 123

[Close]

BLAST of Carg08182-RA vs. TAIR 10
Match: AT2G02850.1 (plantacyanin )

HSP 1 Score: 135.2 bits (339), Expect = 3.6e-32
Identity = 68/129 (52.71%), Postives = 86/129 (66.67%), Query Frame = 0


Query: 1   MAQGRGSAAMGA------VMVCLLVLQFSAMARAAVYTVGGAQGWTFNVASWPKGKRFRA 60
           MA+GRGSA+  A      + V +L+LQ +   +AA YTVG +  WTFN   WPKGK FRA
Sbjct: 1   MAKGRGSASWSARAIVTLMAVSVLLLQ-ADYVQAATYTVGDSGIWTFNAVGWPKGKHFRA 60

Query: 61  GDTLVFNYSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVKGQNYFICSLPGHCQ 120
           GD LVFNY+P  HNVV V    Y+ C  P G+K + +G D+I L KGQN+FIC+ P HC+
Sbjct: 61  GDVLVFNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHCE 120

Query: 121 SGMKIAINA 124
           S MKIA+ A
Sbjct: 121 SDMKIAVTA 128

[Close]

BLAST of Carg08182-RA vs. TAIR 10
Match: AT3G60270.1 (Cupredoxin superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.1e-17
Identity = 49/119 (41.18%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 6   GSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNV--ASWPKGKRFRAGDTLVFNYS 65
           GS A  A+++ LL++   A+  A  + VG   GWT  V   SW   K FR GDTL F Y 
Sbjct: 2   GSTAATALLLLLLLVAVPAVF-AVTFQVGDNDGWTIGVEYTSWVSEKTFRVGDTLEFKYG 61

Query: 66  PAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVK-GQNYFICSLPGHCQSGMKIAI 122
           P +H+V  V++  Y GC   R ++ F  G  +I L K G  +F+C  PGHC  GMK+A+
Sbjct: 62  P-SHSVAVVNKADYDGCETSRPTQSFSDGDTKIDLTKVGAIHFLCLTPGHCSLGMKLAV 118

[Close]

BLAST of Carg08182-RA vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 86.3 bits (212), Expect = 1.9e-17
Identity = 40/113 (35.40%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 13  VMVCLLVLQFSAMARAAVYTVGGAQGWTF--NVASWPKGKRFRAGDTLVFNYSPAAHNVV 72
           +++ +L      +  A  +T+GG  GWT   ++ +W  G+ F  GD LVF+Y  A H+VV
Sbjct: 8   IIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPAAFHDVV 67

Query: 73  AVSRVGYSGCVAPRGSKVFGTGTDQIKL-VKGQNYFICSLPGHCQSGMKIAIN 123
            V++  +  C A +    F  G   + L   G+ YFIC +PGHC  GMK+ +N
Sbjct: 68  EVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVN 120

[Close]

BLAST of Carg08182-RA vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 83.6 bits (205), Expect = 1.2e-16
Identity = 43/121 (35.54%), Postives = 66/121 (54.55%), Query Frame = 0


Query: 7   SAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWT----FNVASWPKGKRFRAGDTLVFNY 66
           +A + A + C++V+    ++ AAVY VG + GWT     +   W   K F  GDT++F Y
Sbjct: 2   AAIIVAALACIVVM--LRLSEAAVYKVGDSAGWTTIANVDYKLWASTKTFHIGDTVLFEY 61

Query: 67  SPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKLVK-GQNYFICSLPGHCQSGMKIAI 123
           +P  HNV+ V+   Y  C   +    F TG D I L   G ++F C +PGHC +G K+ +
Sbjct: 62  NPQFHNVMRVTHPMYRSCNTSKPISTFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDL 120

[Close]

BLAST of Carg08182-RA vs. TAIR 10
Match: AT2G44790.1 (uclacyanin 2 )

HSP 1 Score: 80.1 bits (196), Expect = 1.4e-15
Identity = 51/124 (41.13%), Postives = 64/124 (51.61%), Query Frame = 0


Query: 4   GRGSAAMGAVMVCLLVLQFSAMARAAVYTVGGAQGWTFNV--ASWPKGKRFRAGDTLVFN 63
           G    A+ A    LLVL     A A  YT+     WT  V  + W  GK FR GD L F 
Sbjct: 5   GLSKMAVAAATALLLVLTIVPGAVAVTYTI----EWTTGVDYSGWATGKTFRVGDILEFK 64

Query: 64  YSPAAHNVVAVSRVGYSGCVAPRGSKVFGTGTDQIKL-VKGQNYFICSLPGHCQS--GMK 123
           Y  ++H V  V + GY GC A   ++    G  +I L   G NYFICS PGHC++  GMK
Sbjct: 65  YG-SSHTVDVVDKAGYDGCDASSSTENHSDGDTKIDLKTVGINYFICSTPGHCRTNGGMK 123

The following BLAST results are available for this feature:

BLAST of Carg08182-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6600348.1	1.7e-63	100.00	hypothetical protein SDJN03_05581, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022942619.1	3.1e-62	98.39	basic blue protein-like [Cucurbita moschata]	[more]
XP_023552090.1	7.0e-62	97.58	basic blue protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022983409.1	1.5e-59	92.74	basic blue protein-like [Cucurbita maxima]	[more]
XP_023553060.1	7.0e-54	84.68	basic blue protein-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg08182-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P00303	1.2e-35	69.47	Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P60496	8.4e-34	57.14	Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1	[more]
Q8LG89	5.1e-31	52.71	Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2	[more]
O82081	2.7e-16	35.40	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
Q41001	1.8e-15	37.82	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]

BLAST of Carg08182-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRV0	1.5e-62	98.39	basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111447602 PE=4 SV=1	[more]
A0A6J1J7A3	7.1e-60	92.74	basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111482017 PE=4 SV=1	[more]
A0A6J1E590	7.6e-54	83.87	basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430872 PE=4 SV=1	[more]
A0A6J1J7W1	9.9e-54	83.06	basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483380 PE=4 SV=1	[more]
A0A6J1D6I4	3.2e-52	82.11	basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111017723 PE=4 SV=1	[more]

BLAST of Carg08182-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02850.1	3.6e-32	52.71	plantacyanin	[more]
AT3G60270.1	1.1e-17	41.18	Cupredoxin superfamily protein	[more]
AT2G32300.1	1.9e-17	35.40	uclacyanin 1	[more]
AT5G26330.1	1.2e-16	35.54	Cupredoxin superfamily protein	[more]
AT2G44790.1	1.4e-15	41.13	uclacyanin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 39..117 e-value: 1.9E-24 score: 85.4
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 29..124 score: 34.89505
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 29..123 e-value: 5.0E-31 score: 108.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 29..123
None	No IPR available	PANTHER	PTHR33021:SF345	BASIC BLUE-LIKE PROTEIN	coord: 9..122
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 9..122
IPR028871	Blue (type 1) copper protein, binding site	PROSITE	PS00196	COPPER_BLUE	coord: 101..117
IPR041844	Plantacyanin	CDD	cd11013	Plantacyanin	coord: 29..123 e-value: 2.45225E-52 score: 158.286

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg08182	Carg08182	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08182-RA:1	Carg08182-RA:1	exon
Carg08182-RA:2	Carg08182-RA:2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08182-RA:five_prime_utr	Carg08182-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08182-RA:cds	Carg08182-RA:cds	CDS
Carg08182-RA:cds	Carg08182-RA:cds_2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08182-RA:three_prime_utr	Carg08182-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg08182-RA	Carg08182-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0009055	electron transfer activity