Carg08089-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08089-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionHeat shock 70 kDa protein
LocationCarg_Chr04: 2207151 .. 2210933 (+)
Sequence length2453
RNA-Seq ExpressionCarg08089-RA
SyntenyCarg08089-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACCCTAAACCCTACCTTTCTCCCCTTTTTTCCTCTACCAGTTTCGCCCATGGCCACCGGTGTGCTGCTCCGTTCTCTTCGACGTCGCGATTTAGCCTCATCCTCCATTTCAGCTTACCGATCCGTATGTCTGCTTCTCCTTTCCTGTGATTACTCGATTGTTTGTTCTCTGTTGTAGATTTTTGGTTTGTACTTTTTGAGTTGTCGAGTTTTGTTGCTTTTGGACATATGGGAGCTGTTGTGCTTGTTAATATTTCTGTCGGAACTTTTTATTGTTTGTAATAAATGAAGCTGGAGTGATCGAATTTTGGTTTTGGTTATGGTTTGAAATACGTGATCTGATTAGAACTATGGATTGGGGCTTCTGTTGTGCACGCTCTATCGGATCTGTGTATAACGAATTCGTTGTGATTATTTACTAGTGTGTGGGAATGTCTCGTATTTCGGTCTATTCATGAAGAGAGTTGGGTGGACAGTAGTGTACGGGAATGGTAAAAGGTTGTTTGGTTGGATTGTGACTTTCATGTAGTCAGTAGCTTGGAGATGTTTTTGATTTAGTTGTTGGTGGGAAATATGTCATAAATCTTTTGATGATTTGAGGTGCCCCCCCTCCTTTCTAGTGAGTCTAGGTACGAAGCGGTTTGAATGCGATTCTTTGATTTTGTTAGTTGCGCTCATTTTCATGTCGTACTAACACGACTTTGTTTTTTTACCCATAAAACATAGTCCTTCTTGGCTTTAATCACTAGTCAGTAATTTGAAACTTTGTTGTTCCACCTGTACTGCATGGGATTTTTACTTGCTGGCATATCCACTATCACAATTTTCAGACTTACACGCGTATGTTTATGATGTTGCAGTTAACAAGCAATGCCAAGTCGTCATTGGCCAGGTCTCACCTCAGCCAATCATGGGCAAGTTTGTCCAGGCCATTCAGGTTCTGATGACTTGAAGTGATTAGTTACTTGATTAATGTTATCTGTTGGTTTCCATCATTGCCTGATATTTGTCTTGTCTTCTCCGTGCAGTTCAAAGCCAGCTGGGAGTGATGTTATCGGTATTGATTTGGGTACCACCAATTCTTGTGTTTCTGTAATGGAAGGGAAGGTAAGTCTCAATGTACACACGTGGTTGATTAATGGCTTGAGTGTGCCCATTTTATGCTTCCCTGATTCTGGTTATATGTTTGTAGAATCCAAAAGTTATTGAAAACTCTGAAGGTGCTAGGACCACACCATCAGTGGTTGCTTTCAATCAGAAAGGTGAACTACTTGTAGGAACACCAGCAAAGAGACAGGCAGTTACTAATCCAACTAATACTGTGTTTGGAACCAAACGTCTCATTGGTAGACGCTTTGATGACCCCCAGACACAGAAGGAAATGAAGATGGTACCATACAAAATAGTCAGAGCTCCTAATGGAGATGCTTGGGTTGAAGCAAATGGACAGCAGTACTCACCGAGTCAGATTGGTGCTTTTGTTCTAACAAAGATGAAGGAAACTGCTGAAGCCTATCTCGGAAAGAGCGTGTCCAAGGCTGTAATTACCGTTCCAGCTTATTTCAACGATGCACAGAGACAGGCAACAAAGGATGCTGGCAGGATTGCTGGTTTGGATGTTCAAAGAATTATCAATGAGCCCACTGCTGCTGCACTTTCCTATGGAATGAATAATAAGGAAGGTCTTATTGCTGTCTTTGATCTTGGTGGTGGCACATTCGATGTTTCCATCTTGGAGATATCCAATGGTGTTTTTGAGGTAGAATTTTTCCCTATGTTGTACTTTTTCATAATATGAGTTTTAGATTATTAAATCTTTTTTGCTTCCATAATATCTGAGTTTTACAAGCCTACATTATGTTATTAGGTGAAATCGACAAATGGTGACACATTCTTGGGAGGAGAGGATTTTGACAATGCTCTACTGGACTTCCTGGTTAGTGAGTTCAAGCGAACTGATGGAATTGACCTTACCAAGGACAAGCTTGCCTTGCAGAGGCTTAGGGAGGCTGCAGAGAAGGCAAAGATTGAACTATCATCTACATCGCAGACTGAAATTAACCTGCCATTTATCACTGCTGATGCATCTGGTGCAAAGCATTTGAACATCACTTTGACCAGGTCGAAATTTGAAAGTCTAGTTAGTAACCTGATTGAAAGGACCAAGGCACCATGCAAAAATTGTTTGAAGGATGCCAATATTTCCATCAAAGAAGTGGATGAAGTGCTTTTAGTGGGTGGAATGACTCGTGTACCTAAGGTGCAAGAAGTTGTCACAGAAATTTTTGGTAAGAGCCCCAGTAAAGGGGTGAATCCTGATGAAGCAGTTGCTATGGGAGCTGCAATTCAAGGTGGCATTCTTCGTGGTGATGTCAAAGAATTGCTTCTTTTGGATGTTACCCCACTATCGCTTGGTATTGAGACGCTTGGCGGTATATTTACAAGGTTGATCAGTCGGAACACTACCATTCCAACTAAGAAGAGCCAGGTAATTTCTTCGTCAAGCTTGTTTATAACAGTAATGTATGAGTAAATTCTACTCTGGTTAACCCTTCTGTCAAGTTAATTCTGTTCCTCATTCCCATATTTTCGGAAAGTCAAGTACCTTCCACATGGAGCATTCACGATAATAGATAGGGGTCAATTGGCGATGATATAGTACAATTTAGTTGTAGATGGTAGTTTGAATCAAGTATTTATTGCTGTTGCTTATAAAAAACCAGTAGTACGTTGAGGTTGATCAATTTTGTCCATTGTGGATGATGCATTGTGTAATTATTCGAATTTCCTCATTGCAGGTGTTCTCAACTGCAGCTGACAACCAGACCCAGGTGGGTATCAAGGTATTACAAGGTGAGAGAGAAATGGCTTCTGACAACAAGCTTCTAGGGGAGTTTGAGTTGGTTGGAATTCCTCCCGCTCCAAGAGGAATGCCTCAAGTTGAGGTGACATTCGATATTGATGCCAATGGTATTGTTACTGTCTCGGCCAAGGACAAGTCCACCGGTAAAGAGCAACAAATCACCATTCGATCTTCTGGAGGACTCTCAGATGACGAAATAGAGAAAATGGTGAAGGAAGCGGAGTTGCATGCCCAGAAGGATCAAGAGAGGAAATCTCTTATTGACATCAGAAACAGTGCAGATACCACCATCTACAGCATTGAGAAGAGCTTGGGTGAATACAGGGAGAAAATACCTAGTGAGGTTGCCAAGGAAATCGAGGATGCAGTAGCAGATTTGAGGAAGGCAATGTCTGGAGATGACATCGAAGAGATCAAGGCAAAACTTGACGTCGCCAACAAAGCTGTTTCCAAGATTGGGCAACATATGGCTGGTGGTTCAGGTGGTGATGCTGCTTCTGGAGGGTCTCAGGGTGGCGAACAAGCTTCTGAAGCTGAGTACGAAGAGGTGAAGAAATAAGGCAAGGCGCGCTGCAATGGCGTCCTGATTGTCCTGATTGTTCGGTTGCATGGATTTTCTTTCCCTTATTTTTTACAACCAAAGGGTGCTCTCATCTTATTTTTGCGGTAGAACTAAGAAACCTAGCGGATTTTTGTATCTAATCGTTGGGAGGTTGGTAGAACGTTTCAGATAATACCAGCAGCAGTGTATTGCTGTTGTTTTGCTTTGTTGAATTCATTGTTAAAGAAATTTGTCAGTGATTTGTGTCACTGCCTGCCTGTTTTCGATGACAAAAAACTCGTCTGAATCATGACCATGCTTTTGGTTTACGCTAACTTGATGAATAAACTCACTCGAATGCAAGTTCTCTCTCTTTT

mRNA sequence

AAAACCCTAAACCCTACCTTTCTCCCCTTTTTTCCTCTACCAGTTTCGCCCATGGCCACCGGTGTGCTGCTCCGTTCTCTTCGACGTCGCGATTTAGCCTCATCCTCCATTTCAGCTTACCGATCCTTAACAAGCAATGCCAAGTCGTCATTGGCCAGGTCTCACCTCAGCCAATCATGGGCAAGTTTGTCCAGGCCATTCAGTTCAAAGCCAGCTGGGAGTGATGTTATCGGTATTGATTTGGGTACCACCAATTCTTGTGTTTCTGTAATGGAAGGGAAGAATCCAAAAGTTATTGAAAACTCTGAAGGTGCTAGGACCACACCATCAGTGGTTGCTTTCAATCAGAAAGGTGAACTACTTGTAGGAACACCAGCAAAGAGACAGGCAGTTACTAATCCAACTAATACTGTGTTTGGAACCAAACGTCTCATTGGTAGACGCTTTGATGACCCCCAGACACAGAAGGAAATGAAGATGGTACCATACAAAATAGTCAGAGCTCCTAATGGAGATGCTTGGGTTGAAGCAAATGGACAGCAGTACTCACCGAGTCAGATTGGTGCTTTTGTTCTAACAAAGATGAAGGAAACTGCTGAAGCCTATCTCGGAAAGAGCGTGTCCAAGGCTGTAATTACCGTTCCAGCTTATTTCAACGATGCACAGAGACAGGCAACAAAGGATGCTGGCAGGATTGCTGGTTTGGATGTTCAAAGAATTATCAATGAGCCCACTGCTGCTGCACTTTCCTATGGAATGAATAATAAGGAAGGTCTTATTGCTGTCTTTGATCTTGGTGGTGGCACATTCGATGTTTCCATCTTGGAGATATCCAATGGTGTTTTTGAGGTGAAATCGACAAATGGTGACACATTCTTGGGAGGAGAGGATTTTGACAATGCTCTACTGGACTTCCTGGTTAGTGAGTTCAAGCGAACTGATGGAATTGACCTTACCAAGGACAAGCTTGCCTTGCAGAGGCTTAGGGAGGCTGCAGAGAAGGCAAAGATTGAACTATCATCTACATCGCAGACTGAAATTAACCTGCCATTTATCACTGCTGATGCATCTGGTGCAAAGCATTTGAACATCACTTTGACCAGGTCGAAATTTGAAAGTCTAGTTAGTAACCTGATTGAAAGGACCAAGGCACCATGCAAAAATTGTTTGAAGGATGCCAATATTTCCATCAAAGAAGTGGATGAAGTGCTTTTAGTGGGTGGAATGACTCGTGTACCTAAGGTGCAAGAAGTTGTCACAGAAATTTTTGGTAAGAGCCCCAGTAAAGGGGTGAATCCTGATGAAGCAGTTGCTATGGGAGCTGCAATTCAAGGTGGCATTCTTCGTGGTGATGTCAAAGAATTGCTTCTTTTGGATGTTACCCCACTATCGCTTGGTATTGAGACGCTTGGCGGTATATTTACAAGGTTGATCAGTCGGAACACTACCATTCCAACTAAGAAGAGCCAGGTGTTCTCAACTGCAGCTGACAACCAGACCCAGGTGGGTATCAAGGTATTACAAGGTGAGAGAGAAATGGCTTCTGACAACAAGCTTCTAGGGGAGTTTGAGTTGGTTGGAATTCCTCCCGCTCCAAGAGGAATGCCTCAAGTTGAGGTGACATTCGATATTGATGCCAATGGTATTGTTACTGTCTCGGCCAAGGACAAGTCCACCGGTAAAGAGCAACAAATCACCATTCGATCTTCTGGAGGACTCTCAGATGACGAAATAGAGAAAATGGTGAAGGAAGCGGAGTTGCATGCCCAGAAGGATCAAGAGAGGAAATCTCTTATTGACATCAGAAACAGTGCAGATACCACCATCTACAGCATTGAGAAGAGCTTGGGTGAATACAGGGAGAAAATACCTAGTGAGGTTGCCAAGGAAATCGAGGATGCAGTAGCAGATTTGAGGAAGGCAATGTCTGGAGATGACATCGAAGAGATCAAGGCAAAACTTGACGTCGCCAACAAAGCTGTTTCCAAGATTGGGCAACATATGGCTGGTGGTTCAGGTGGTGATGCTGCTTCTGGAGGGTCTCAGGGTGGCGAACAAGCTTCTGAAGCTGAGTACGAAGAGGTGAAGAAATAAGGCAAGGCGCGCTGCAATGGCGTCCTGATTGTCCTGATTGTTCGGTTGCATGGATTTTCTTTCCCTTATTTTTTACAACCAAAGGGTGCTCTCATCTTATTTTTGCGGTAGAACTAAGAAACCTAGCGGATTTTTGTATCTAATCGTTGGGAGGTTGGTAGAACGTTTCAGATAATACCAGCAGCAGTGTATTGCTGTTGTTTTGCTTTGTTGAATTCATTGTTAAAGAAATTTGTCAGTGATTTGTGTCACTGCCTGCCTGTTTTCGATGACAAAAAACTCGTCTGAATCATGACCATGCTTTTGGTTTACGCTAACTTGATGAATAAACTCACTCGAATGCAAGTTCTCTCTCTTTT

Coding sequence (CDS)

ATGGCCACCGGTGTGCTGCTCCGTTCTCTTCGACGTCGCGATTTAGCCTCATCCTCCATTTCAGCTTACCGATCCTTAACAAGCAATGCCAAGTCGTCATTGGCCAGGTCTCACCTCAGCCAATCATGGGCAAGTTTGTCCAGGCCATTCAGTTCAAAGCCAGCTGGGAGTGATGTTATCGGTATTGATTTGGGTACCACCAATTCTTGTGTTTCTGTAATGGAAGGGAAGAATCCAAAAGTTATTGAAAACTCTGAAGGTGCTAGGACCACACCATCAGTGGTTGCTTTCAATCAGAAAGGTGAACTACTTGTAGGAACACCAGCAAAGAGACAGGCAGTTACTAATCCAACTAATACTGTGTTTGGAACCAAACGTCTCATTGGTAGACGCTTTGATGACCCCCAGACACAGAAGGAAATGAAGATGGTACCATACAAAATAGTCAGAGCTCCTAATGGAGATGCTTGGGTTGAAGCAAATGGACAGCAGTACTCACCGAGTCAGATTGGTGCTTTTGTTCTAACAAAGATGAAGGAAACTGCTGAAGCCTATCTCGGAAAGAGCGTGTCCAAGGCTGTAATTACCGTTCCAGCTTATTTCAACGATGCACAGAGACAGGCAACAAAGGATGCTGGCAGGATTGCTGGTTTGGATGTTCAAAGAATTATCAATGAGCCCACTGCTGCTGCACTTTCCTATGGAATGAATAATAAGGAAGGTCTTATTGCTGTCTTTGATCTTGGTGGTGGCACATTCGATGTTTCCATCTTGGAGATATCCAATGGTGTTTTTGAGGTGAAATCGACAAATGGTGACACATTCTTGGGAGGAGAGGATTTTGACAATGCTCTACTGGACTTCCTGGTTAGTGAGTTCAAGCGAACTGATGGAATTGACCTTACCAAGGACAAGCTTGCCTTGCAGAGGCTTAGGGAGGCTGCAGAGAAGGCAAAGATTGAACTATCATCTACATCGCAGACTGAAATTAACCTGCCATTTATCACTGCTGATGCATCTGGTGCAAAGCATTTGAACATCACTTTGACCAGGTCGAAATTTGAAAGTCTAGTTAGTAACCTGATTGAAAGGACCAAGGCACCATGCAAAAATTGTTTGAAGGATGCCAATATTTCCATCAAAGAAGTGGATGAAGTGCTTTTAGTGGGTGGAATGACTCGTGTACCTAAGGTGCAAGAAGTTGTCACAGAAATTTTTGGTAAGAGCCCCAGTAAAGGGGTGAATCCTGATGAAGCAGTTGCTATGGGAGCTGCAATTCAAGGTGGCATTCTTCGTGGTGATGTCAAAGAATTGCTTCTTTTGGATGTTACCCCACTATCGCTTGGTATTGAGACGCTTGGCGGTATATTTACAAGGTTGATCAGTCGGAACACTACCATTCCAACTAAGAAGAGCCAGGTGTTCTCAACTGCAGCTGACAACCAGACCCAGGTGGGTATCAAGGTATTACAAGGTGAGAGAGAAATGGCTTCTGACAACAAGCTTCTAGGGGAGTTTGAGTTGGTTGGAATTCCTCCCGCTCCAAGAGGAATGCCTCAAGTTGAGGTGACATTCGATATTGATGCCAATGGTATTGTTACTGTCTCGGCCAAGGACAAGTCCACCGGTAAAGAGCAACAAATCACCATTCGATCTTCTGGAGGACTCTCAGATGACGAAATAGAGAAAATGGTGAAGGAAGCGGAGTTGCATGCCCAGAAGGATCAAGAGAGGAAATCTCTTATTGACATCAGAAACAGTGCAGATACCACCATCTACAGCATTGAGAAGAGCTTGGGTGAATACAGGGAGAAAATACCTAGTGAGGTTGCCAAGGAAATCGAGGATGCAGTAGCAGATTTGAGGAAGGCAATGTCTGGAGATGACATCGAAGAGATCAAGGCAAAACTTGACGTCGCCAACAAAGCTGTTTCCAAGATTGGGCAACATATGGCTGGTGGTTCAGGTGGTGATGCTGCTTCTGGAGGGTCTCAGGGTGGCGAACAAGCTTCTGAAGCTGAGTACGAAGAGGTGAAGAAATAA

Protein sequence

MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVIGIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNTVFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKETAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKEGLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGIDLTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSNLIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAVAMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAADNQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDKSTGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLGEYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAASGGSQGGEQASEAEYEEVKK
Homology
BLAST of Carg08089-RA vs. NCBI nr
Match: KAG7030913.1 (hypothetical protein SDJN02_04950 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1276.5 bits (3302), Expect = 0.0e+00
Identity = 680/680 (100.00%), Postives = 680/680 (100.00%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI
Sbjct: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA
Sbjct: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQASEAEYEEVKK
Sbjct: 661 SGGSQGGEQASEAEYEEVKK 680

BLAST of Carg08089-RA vs. NCBI nr
Match: XP_022943125.1 (heat shock 70 kDa protein, mitochondrial-like [Cucurbita moschata] >KAG6600255.1 hypothetical protein SDJN03_05488, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 679/680 (99.85%), Postives = 679/680 (99.85%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI
Sbjct: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTD ID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDAID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA
Sbjct: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQASEAEYEEVKK
Sbjct: 661 SGGSQGGEQASEAEYEEVKK 680

BLAST of Carg08089-RA vs. NCBI nr
Match: XP_023515841.1 (heat shock 70 kDa protein, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1271.9 bits (3290), Expect = 0.0e+00
Identity = 675/680 (99.26%), Postives = 680/680 (100.00%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAG++VI
Sbjct: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGNEVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDAN+SIKEVDEVLLVGGMTRVPKVQ+VVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDANVSIKEVDEVLLVGGMTRVPKVQDVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLD+ANKAVSKIGQHMAGGSGGDAA
Sbjct: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDIANKAVSKIGQHMAGGSGGDAA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQASEAEYEEVKK
Sbjct: 661 SGGSQGGEQASEAEYEEVKK 680

BLAST of Carg08089-RA vs. NCBI nr
Match: XP_022999746.1 (heat shock 70 kDa protein, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 673/680 (98.97%), Postives = 677/680 (99.56%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATGVLLRSLRRRDLASSSISAYRSLTSNAK SL+RSHLSQSW SLSRPFSSKPAG+DVI
Sbjct: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKPSLSRSHLSQSWTSLSRPFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLI+RNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLINRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEV KEIEDAVADLRKAMSGD+IEEIKAKLDVANKAVSKIGQHMAGGSGGDAA
Sbjct: 601 EYREKIPSEVTKEIEDAVADLRKAMSGDNIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQASEAEYEEVKK
Sbjct: 661 SGGSQGGEQASEAEYEEVKK 680

BLAST of Carg08089-RA vs. NCBI nr
Match: XP_022922655.1 (heat shock 70 kDa protein, mitochondrial [Cucurbita moschata] >KAG6576887.1 hypothetical protein SDJN03_24461, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1233.4 bits (3190), Expect = 0.0e+00
Identity = 653/680 (96.03%), Postives = 670/680 (98.53%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATG LLRSLRRRDLASSS++AYRSLTSNAK S   SHLSQSWA+LSRPFSSKPAG+DVI
Sbjct: 1   MATGALLRSLRRRDLASSSLAAYRSLTSNAKPSWGNSHLSQSWATLSRPFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDV RIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVLRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRT+GID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTEGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLV++
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVNH 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDA+ISIK+VDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDASISIKDVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRG+PQ+EVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGLPQIEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLS+DEIEKMVKEAELHAQKDQERK+LIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSEDEIEKMVKEAELHAQKDQERKALIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIEDAVA+LRKAMS D+IEEIK+KLD ANKAVSKIGQHMAGGSGG +A
Sbjct: 601 EYREKIPSEVAKEIEDAVAELRKAMSEDNIEEIKSKLDAANKAVSKIGQHMAGGSGGGSA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQA EAEYEEVKK
Sbjct: 661 SGGSQGGEQAPEAEYEEVKK 680

BLAST of Carg08089-RA vs. ExPASy Swiss-Prot
Match: Q01899 (Heat shock 70 kDa protein, mitochondrial OS=Phaseolus vulgaris OX=3885 PE=2 SV=1)

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 613/676 (90.68%), Postives = 657/676 (97.19%), Query Frame = 0

Query: 6   LLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVIGIDLG 65
           +LRSLRRRD+AS++ SAYRSLT + K     ++++Q W+ L+RPFSS+PAG+DVIGIDLG
Sbjct: 4   VLRSLRRRDVASATFSAYRSLTGSTKP----AYVAQKWSCLARPFSSRPAGNDVIGIDLG 63

Query: 66  TTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNTVFGTK 125
           TTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNTVFGTK
Sbjct: 64  TTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNTVFGTK 123

Query: 126 RLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKETAEAY 185
           RLIGRRFDDPQTQKEMKMVP+KIV+APNGDAWVEANGQQYSPSQIGAFVLTKMKETAEAY
Sbjct: 124 RLIGRRFDDPQTQKEMKMVPFKIVKAPNGDAWVEANGQQYSPSQIGAFVLTKMKETAEAY 183

Query: 186 LGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKEGLIAV 245
           LGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKEGLIAV
Sbjct: 184 LGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKEGLIAV 243

Query: 246 FDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGIDLTKDK 305
           FDLGGGTFDVSILEISNGVFEVK+TNGDTFLGGEDFDNALLDFLV+EFKRT+ IDL+KD+
Sbjct: 244 FDLGGGTFDVSILEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKRTESIDLSKDR 303

Query: 306 LALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSNLIERT 365
           LALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFE+LV++LIERT
Sbjct: 304 LALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFEALVNHLIERT 363

Query: 366 KAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAVAMGAA 425
           KAPCK+CLKDAN+SIK+VDEVLLVGGMTRVPKVQEVV  IFGKSPSKGVNPDEAVAMGAA
Sbjct: 364 KAPCKSCLKDANVSIKDVDEVLLVGGMTRVPKVQEVVLNIFGKSPSKGVNPDEAVAMGAA 423

Query: 426 IQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAADNQTQV 485
           IQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLI+RNTTIPTKKSQVFSTAADNQTQV
Sbjct: 424 IQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLINRNTTIPTKKSQVFSTAADNQTQV 483

Query: 486 GIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDKSTGKE 545
           GIKVLQGEREMASDNK+LGEF+LVGIPPAPRG+PQ+EVTFDIDANGIVTVSAKDKSTGKE
Sbjct: 484 GIKVLQGEREMASDNKMLGEFDLVGIPPAPRGLPQIEVTFDIDANGIVTVSAKDKSTGKE 543

Query: 546 QQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLGEYREK 605
           QQITIRSSGGLS+DEIEKMVKEAELHAQKDQERK+LIDIRNSADTTIYSIEKSLGEYREK
Sbjct: 544 QQITIRSSGGLSEDEIEKMVKEAELHAQKDQERKTLIDIRNSADTTIYSIEKSLGEYREK 603

Query: 606 IPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAASGGSQ 665
           IPSE AKEIEDAV+DLRKAMSGD+++EIK+KLD ANKAVSKIG+HM+GGS G +++GGSQ
Sbjct: 604 IPSETAKEIEDAVSDLRKAMSGDNVDEIKSKLDAANKAVSKIGEHMSGGSSGGSSAGGSQ 663

Query: 666 -GGEQASEAEYEEVKK 681
            GG+QA EAEYEEVKK
Sbjct: 664 GGGDQAPEAEYEEVKK 675

BLAST of Carg08089-RA vs. ExPASy Swiss-Prot
Match: P37900 (Heat shock 70 kDa protein, mitochondrial OS=Pisum sativum OX=3888 GN=HSP1 PE=2 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 609/675 (90.22%), Postives = 651/675 (96.44%), Query Frame = 0

Query: 6   LLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVIGIDLG 65
           LLRSL+RR+L+SSS+SA+RSLT + K+S A   L    ASL+RPFSS+PAG+DVIGIDLG
Sbjct: 5   LLRSLQRRNLSSSSVSAFRSLTGSTKTSYATHKL----ASLTRPFSSRPAGNDVIGIDLG 64

Query: 66  TTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNTVFGTK 125
           TTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQK ELLVGTPAKRQAVTNPTNT+FGTK
Sbjct: 65  TTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKSELLVGTPAKRQAVTNPTNTLFGTK 124

Query: 126 RLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKETAEAY 185
           RLIGRRFDD QTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTK+KETAEAY
Sbjct: 125 RLIGRRFDDAQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKIKETAEAY 184

Query: 186 LGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKEGLIAV 245
           LGK++SKAV+TVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKEGLIAV
Sbjct: 185 LGKTISKAVVTVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKEGLIAV 244

Query: 246 FDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGIDLTKDK 305
           FDLGGGTFDVSILEISNGVFEVK+TNGDTFLGGEDFDNALLDFLVSEFKRT+ IDL KDK
Sbjct: 245 FDLGGGTFDVSILEISNGVFEVKATNGDTFLGGEDFDNALLDFLVSEFKRTESIDLAKDK 304

Query: 306 LALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSNLIERT 365
           LALQRLREAAEKAKIELSSTSQTEINLPFI+ADASGAKHLNITLTRSKFE+LV+NLIERT
Sbjct: 305 LALQRLREAAEKAKIELSSTSQTEINLPFISADASGAKHLNITLTRSKFEALVNNLIERT 364

Query: 366 KAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAVAMGAA 425
           KAPCK+CLKDANISIK+VDEVLLVGGMTRVPKVQ+VV+EIFGKSPSKGVNPDEAVAMGAA
Sbjct: 365 KAPCKSCLKDANISIKDVDEVLLVGGMTRVPKVQQVVSEIFGKSPSKGVNPDEAVAMGAA 424

Query: 426 IQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAADNQTQV 485
           +QGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAADNQTQV
Sbjct: 425 LQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAADNQTQV 484

Query: 486 GIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDKSTGKE 545
           GIKVLQGEREMA+DNK LGEF+LVGIPPAPRG+PQ+EVTFDIDANGIVTVSAKDKSTGKE
Sbjct: 485 GIKVLQGEREMAADNKSLGEFDLVGIPPAPRGLPQIEVTFDIDANGIVTVSAKDKSTGKE 544

Query: 546 QQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLGEYREK 605
           QQITIRSSGGLSDDEI+KMVKEAELHAQ+DQERK+LIDIRNSADT+IYSIEKSL EYREK
Sbjct: 545 QQITIRSSGGLSDDEIDKMVKEAELHAQRDQERKALIDIRNSADTSIYSIEKSLAEYREK 604

Query: 606 IPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAASGGSQ 665
           IP+EVAKEIEDAV+DLR AM+G++ ++IKAKLD ANKAVSKIGQHM+GGS G  + GGSQ
Sbjct: 605 IPAEVAKEIEDAVSDLRTAMAGENADDIKAKLDAANKAVSKIGQHMSGGSSGGPSEGGSQ 664

Query: 666 GGEQASEAEYEEVKK 681
           GGEQA EAEYEEVKK
Sbjct: 665 GGEQAPEAEYEEVKK 675

BLAST of Carg08089-RA vs. ExPASy Swiss-Prot
Match: Q9LDZ0 (Heat shock 70 kDa protein 10, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HSP70-10 PE=1 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 592/682 (86.80%), Postives = 646/682 (94.72%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MAT  LLRS+RRR++ SS  SAYR L+S+ K+SL  S+L Q++ S SR FSSKPAG+DVI
Sbjct: 1   MATAALLRSIRRREVVSSPFSAYRCLSSSGKASLNSSYLGQNFRSFSRAFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCV+VMEGKNPKVIEN+EGARTTPSVVAFN KGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVAVMEGKNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           V GTKRLIGR+FDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAF+LTKMKE
Sbjct: 121 VSGTKRLIGRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSV+KAV+TVPAYFNDAQRQATKDAGRIAGLDV+RIINEPTAAALSYGM NKE
Sbjct: 181 TAEAYLGKSVTKAVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVS+LEISNGVFEVK+TNGDTFLGGEDFDNALLDFLV+EFK T+GID
Sbjct: 241 GLIAVFDLGGGTFDVSVLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTTEGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           L KD+LALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKH NITLTRS+FE+LV++
Sbjct: 301 LAKDRLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNH 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERT+ PCKNCLKDA IS KEVDEVLLVGGMTRVPKVQ +V EIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTRDPCKNCLKDAGISAKEVDEVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAA+QGGILRGDVKELLLLDVTPLSLGIETLGG+FTRLI+RNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAALQGGILRGDVKELLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGI+VLQGEREMA+DNKLLGEF+LVGIPP+PRG+PQ+EVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIRVLQGEREMATDNKLLGEFDLVGIPPSPRGVPQIEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           +TGK QQITIRSSGGLS+D+I+KMV+EAELHAQKD+ERK LID +N+ADTTIYSIEKSLG
Sbjct: 541 TTGKVQQITIRSSGGLSEDDIQKMVREAELHAQKDKERKELIDTKNTADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSE+AKEIEDAVADLR A SGDD+ EIKAK++ ANKAVSKIG+HM+GGSGG +A
Sbjct: 601 EYREKIPSEIAKEIEDAVADLRSASSGDDLNEIKAKIEAANKAVSKIGEHMSGGSGGGSA 660

Query: 661 -SGGSQGG-EQASEAEYEEVKK 681
             GGS+GG +QA EAEYEEVKK
Sbjct: 661 PGGGSEGGSDQAPEAEYEEVKK 682

BLAST of Carg08089-RA vs. ExPASy Swiss-Prot
Match: Q08276 (Heat shock 70 kDa protein, mitochondrial OS=Solanum tuberosum OX=4113 GN=HSP68 PE=2 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 572/682 (83.87%), Postives = 630/682 (92.38%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MAT  LLRSLRRR+ A+SSISAYR+L SN K S   S +   WA L+RPFSSKPAG+++I
Sbjct: 1   MATAALLRSLRRREFATSSISAYRTLASNTKPSWCPSLVGAKWAGLARPFSSKPAGNEII 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCV+VMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVAVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           + GTKRLIGRRFDDPQTQKEMKMVPYKIVR  NGDAWVEANGQQYSP+QIGAF+LTKMKE
Sbjct: 121 LSGTKRLIGRRFDDPQTQKEMKMVPYKIVRGSNGDAWVEANGQQYSPTQIGAFILTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKS++KAVITVPAYFNDAQRQA KDAG IAGLDVQRIINEPTAAALSYGMN+KE
Sbjct: 181 TAEAYLGKSINKAVITVPAYFNDAQRQAIKDAGAIAGLDVQRIINEPTAAALSYGMNSKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GL+AVFDLGGGTFDVSILEISNGVFEVK+TNGDTFLGGEDFDNALL+FLVSEFKRT+GID
Sbjct: 241 GLVAVFDLGGGTFDVSILEISNGVFEVKATNGDTFLGGEDFDNALLEFLVSEFKRTEGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           L+KDKLALQRLREAAEKAKIELSSTSQT+INLPFITADASGAKHLNITLTRSKFE+LV++
Sbjct: 301 LSKDKLALQRLREAAEKAKIELSSTSQTDINLPFITADASGAKHLNITLTRSKFETLVNH 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERT+ PCKNCLKDA +S+K+VDEVLLVGGMTRVPKVQE+V+EIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTRNPCKNCLKDAGVSLKDVDEVLLVGGMTRVPKVQEIVSEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAA+QGGILRGDVKELLLLDVTPL+ GIETLGGIFTRLI+RNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAALQGGILRGDVKELLLLDVTPLARGIETLGGIFTRLINRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRG-MPQVEVTFDIDANGIVTVSAKD 540
           NQTQVGIKVLQGEREMASDNKLLGEF+LVGIPPAP+G  PQ+EV FDIDANG+VTVSAKD
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFDLVGIPPAPKGYCPQIEVIFDIDANGMVTVSAKD 540

Query: 541 KSTGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSL 600
           K+T KEQQITIRSSGGLS+DEI+KMV+EAE+HAQ+ +  + L+       TTIYSIEKSL
Sbjct: 541 KATSKEQQITIRSSGGLSEDEIDKMVREAEMHAQRIKNARHLLISGIVQSTTIYSIEKSL 600

Query: 601 GEYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGG-SGGD 660
            EY+EK+P EV  EIE A++DLR AM  ++I++IKAKLD ANKAVSKIG+HMAGG SGG 
Sbjct: 601 SEYKEKVPKEVVTEIETAISDLRAAMGTENIDDIKAKLDAANKAVSKIGEHMAGGSSGGA 660

Query: 661 AASGGSQGGEQASEAEYEEVKK 681
           +  GG+QGG+Q  EAEYEEVKK
Sbjct: 661 SGGGGAQGGDQPPEAEYEEVKK 682

BLAST of Carg08089-RA vs. ExPASy Swiss-Prot
Match: Q8GUM2 (Heat shock 70 kDa protein 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HSP70-9 PE=1 SV=1)

HSP 1 Score: 1069.7 bits (2765), Expect = 1.4e-311
Identity = 557/681 (81.79%), Postives = 626/681 (91.92%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MA+  LLRS RRR++  +S+SA++S+++N K+S     +      L+RPF S+P G+DVI
Sbjct: 1   MASVALLRSFRRREVQMASVSAFKSVSANGKNS-----MFGKLGYLARPFCSRPVGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGK  +VIEN+EG+RTTPSVVA NQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKTARVIENAEGSRTTPSVVAMNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           +FG+KRLIGRRFDDPQTQKEMKMVPYKIV+APNGDAWVEANGQ++SPSQIGA VLTKMKE
Sbjct: 121 IFGSKRLIGRRFDDPQTQKEMKMVPYKIVKAPNGDAWVEANGQKFSPSQIGANVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKS++KAV+TVPAYFNDAQRQATKDAG+IAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSINKAVVTVPAYFNDAQRQATKDAGKIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           G+IAVFDLGGGTFDVSILEIS+GVFEVK+TNGDTFLGGEDFDN LL++LV+EFKR+D ID
Sbjct: 241 GVIAVFDLGGGTFDVSILEISSGVFEVKATNGDTFLGGEDFDNTLLEYLVNEFKRSDNID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKD LALQRLREAAEKAKIELSST+QTEINLPFITADASGAKHLNITLTRSKFE LV  
Sbjct: 301 LTKDNLALQRLREAAEKAKIELSSTTQTEINLPFITADASGAKHLNITLTRSKFEGLVGK 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERT++PC+NCLKDA ++IKEVDEVLLVGGMTRVPKVQE+V+EIFGKSP KGVNPDEAV
Sbjct: 361 LIERTRSPCQNCLKDAGVTIKEVDEVLLVGGMTRVPKVQEIVSEIFGKSPCKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVK+LLLLDV PLSLGIETLG +FT+LI RNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKDLLLLDVVPLSLGIETLGAVFTKLIPRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQ QVGIKVLQGEREMA+DNK+LGEF+LVGIPPAPRGMPQ+EVTFDIDANGI TVSAKDK
Sbjct: 481 NQMQVGIKVLQGEREMAADNKVLGEFDLVGIPPAPRGMPQIEVTFDIDANGITTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           +TGKEQ ITIRSSGGLSDDEI +MVKEAEL+AQKDQE+K LID+RNSADTTIYS+EKSL 
Sbjct: 541 ATGKEQNITIRSSGGLSDDEINRMVKEAELNAQKDQEKKQLIDLRNSADTTIYSVEKSLS 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHM---AGGSGG 660
           EYREKIP+E+A EIE AV+DLR AM+G+D+E+IKAK++ ANKAVSKIG+HM   +G SG 
Sbjct: 601 EYREKIPAEIASEIETAVSDLRTAMAGEDVEDIKAKVEAANKAVSKIGEHMSKGSGSSGS 660

Query: 661 DAASG-GSQGGEQASEAEYEE 678
           D +SG G+ G EQ  EAE+EE
Sbjct: 661 DGSSGEGTSGTEQTPEAEFEE 676

BLAST of Carg08089-RA vs. ExPASy TrEMBL
Match: A0A6J1FXE8 (heat shock 70 kDa protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111447951 PE=3 SV=1)

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 679/680 (99.85%), Postives = 679/680 (99.85%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI
Sbjct: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTD ID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDAID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA
Sbjct: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQASEAEYEEVKK
Sbjct: 661 SGGSQGGEQASEAEYEEVKK 680

BLAST of Carg08089-RA vs. ExPASy TrEMBL
Match: A0A6J1KDZ1 (heat shock 70 kDa protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111494001 PE=3 SV=1)

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 673/680 (98.97%), Postives = 677/680 (99.56%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATGVLLRSLRRRDLASSSISAYRSLTSNAK SL+RSHLSQSW SLSRPFSSKPAG+DVI
Sbjct: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKPSLSRSHLSQSWTSLSRPFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLI+RNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLINRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEV KEIEDAVADLRKAMSGD+IEEIKAKLDVANKAVSKIGQHMAGGSGGDAA
Sbjct: 601 EYREKIPSEVTKEIEDAVADLRKAMSGDNIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQASEAEYEEVKK
Sbjct: 661 SGGSQGGEQASEAEYEEVKK 680

BLAST of Carg08089-RA vs. ExPASy TrEMBL
Match: A0A6J1E9E1 (heat shock 70 kDa protein, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111430597 PE=3 SV=1)

HSP 1 Score: 1233.4 bits (3190), Expect = 0.0e+00
Identity = 653/680 (96.03%), Postives = 670/680 (98.53%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATG LLRSLRRRDLASSS++AYRSLTSNAK S   SHLSQSWA+LSRPFSSKPAG+DVI
Sbjct: 1   MATGALLRSLRRRDLASSSLAAYRSLTSNAKPSWGNSHLSQSWATLSRPFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDV RIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVLRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRT+GID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTEGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLV++
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVNH 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDA+ISIK+VDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDASISIKDVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRG+PQ+EVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGLPQIEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLS+DEIEKMVKEAELHAQKDQERK+LIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSEDEIEKMVKEAELHAQKDQERKALIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIEDAVA+LRKAMS D+IEEIK+KLD ANKAVSKIGQHMAGGSGG +A
Sbjct: 601 EYREKIPSEVAKEIEDAVAELRKAMSEDNIEEIKSKLDAANKAVSKIGQHMAGGSGGGSA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQA EAEYEEVKK
Sbjct: 661 SGGSQGGEQAPEAEYEEVKK 680

BLAST of Carg08089-RA vs. ExPASy TrEMBL
Match: A0A6J1JBC5 (heat shock 70 kDa protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111483457 PE=3 SV=1)

HSP 1 Score: 1230.7 bits (3183), Expect = 0.0e+00
Identity = 652/680 (95.88%), Postives = 669/680 (98.38%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATG LLRSLRRRDLASSS++AYRSLTSNAK S   SHLSQSWA+LSRPFSSKPAG+DVI
Sbjct: 1   MATGALLRSLRRRDLASSSLAAYRSLTSNAKPSWGNSHLSQSWATLSRPFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDV RIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVLRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRT+GID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTEGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLV++
Sbjct: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVNH 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKDA+ISIK+VDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDASISIKDVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRG+PQ+EVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGLPQIEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLS+DEI KMVKEAELHAQKDQERK+LIDIRNSADTTIYSIEKSLG
Sbjct: 541 STGKEQQITIRSSGGLSEDEIVKMVKEAELHAQKDQERKALIDIRNSADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIEDAVA+LRKAMS D+IEEIK+KLD ANKAVSKIGQHMAGGSGG +A
Sbjct: 601 EYREKIPSEVAKEIEDAVAELRKAMSEDNIEEIKSKLDAANKAVSKIGQHMAGGSGGGSA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQA EAEYEEVKK
Sbjct: 661 SGGSQGGEQAPEAEYEEVKK 680

BLAST of Carg08089-RA vs. ExPASy TrEMBL
Match: A0A6J1D541 (heat shock 70 kDa protein, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017686 PE=3 SV=1)

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 652/680 (95.88%), Postives = 668/680 (98.24%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MATGVLLRSLRRRD+ASSS+SAYRSLTSNAK S  +S LSQSWA+LSRPFSSKPAG+DVI
Sbjct: 1   MATGVLLRSLRRRDVASSSLSAYRSLTSNAKPSWTKSQLSQSWATLSRPFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE
Sbjct: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGK+VSKAVITVPAYFNDAQRQATKDAGRIAGLDV RIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKTVSKAVITVPAYFNDAQRQATKDAGRIAGLDVLRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVSILEISNGVFEVK+TNGDTFLGGEDFDNALLDFLVSEFKRT+ ID
Sbjct: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKATNGDTFLGGEDFDNALLDFLVSEFKRTEAID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKD+LALQRLREAAEKAKIELSSTSQTEINLPFITADA+GAKHLNITLTRSKFESLVS+
Sbjct: 301 LTKDRLALQRLREAAEKAKIELSSTSQTEINLPFITADATGAKHLNITLTRSKFESLVSH 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERTKAPCKNCLKD NISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTKAPCKNCLKDGNISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQ+EVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQIEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           STGKEQQITIRSSGGLS+DEIEKMVKEAELHAQKDQERK+LIDIRNSADTTIYSIEKSL 
Sbjct: 541 STGKEQQITIRSSGGLSEDEIEKMVKEAELHAQKDQERKALIDIRNSADTTIYSIEKSLA 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSEVAKEIED+VADLRKAMS DDIE IK+KLD ANKAVSKIGQHMAGGSGG AA
Sbjct: 601 EYREKIPSEVAKEIEDSVADLRKAMSEDDIEAIKSKLDAANKAVSKIGQHMAGGSGGGAA 660

Query: 661 SGGSQGGEQASEAEYEEVKK 681
           SGGSQGGEQASEAEYEEVKK
Sbjct: 661 SGGSQGGEQASEAEYEEVKK 680

BLAST of Carg08089-RA vs. TAIR 10
Match: AT5G09590.1 (mitochondrial HSO70 2 )

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 592/682 (86.80%), Postives = 646/682 (94.72%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MAT  LLRS+RRR++ SS  SAYR L+S+ K+SL  S+L Q++ S SR FSSKPAG+DVI
Sbjct: 1   MATAALLRSIRRREVVSSPFSAYRCLSSSGKASLNSSYLGQNFRSFSRAFSSKPAGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCV+VMEGKNPKVIEN+EGARTTPSVVAFN KGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVAVMEGKNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           V GTKRLIGR+FDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAF+LTKMKE
Sbjct: 121 VSGTKRLIGRKFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFILTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKSV+KAV+TVPAYFNDAQRQATKDAGRIAGLDV+RIINEPTAAALSYGM NKE
Sbjct: 181 TAEAYLGKSVTKAVVTVPAYFNDAQRQATKDAGRIAGLDVERIINEPTAAALSYGMTNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           GLIAVFDLGGGTFDVS+LEISNGVFEVK+TNGDTFLGGEDFDNALLDFLV+EFK T+GID
Sbjct: 241 GLIAVFDLGGGTFDVSVLEISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTTEGID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           L KD+LALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKH NITLTRS+FE+LV++
Sbjct: 301 LAKDRLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHFNITLTRSRFETLVNH 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERT+ PCKNCLKDA IS KEVDEVLLVGGMTRVPKVQ +V EIFGKSPSKGVNPDEAV
Sbjct: 361 LIERTRDPCKNCLKDAGISAKEVDEVLLVGGMTRVPKVQSIVAEIFGKSPSKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAA+QGGILRGDVKELLLLDVTPLSLGIETLGG+FTRLI+RNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAALQGGILRGDVKELLLLDVTPLSLGIETLGGVFTRLITRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQTQVGI+VLQGEREMA+DNKLLGEF+LVGIPP+PRG+PQ+EVTFDIDANGIVTVSAKDK
Sbjct: 481 NQTQVGIRVLQGEREMATDNKLLGEFDLVGIPPSPRGVPQIEVTFDIDANGIVTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           +TGK QQITIRSSGGLS+D+I+KMV+EAELHAQKD+ERK LID +N+ADTTIYSIEKSLG
Sbjct: 541 TTGKVQQITIRSSGGLSEDDIQKMVREAELHAQKDKERKELIDTKNTADTTIYSIEKSLG 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAGGSGGDAA 660
           EYREKIPSE+AKEIEDAVADLR A SGDD+ EIKAK++ ANKAVSKIG+HM+GGSGG +A
Sbjct: 601 EYREKIPSEIAKEIEDAVADLRSASSGDDLNEIKAKIEAANKAVSKIGEHMSGGSGGGSA 660

Query: 661 -SGGSQGG-EQASEAEYEEVKK 681
             GGS+GG +QA EAEYEEVKK
Sbjct: 661 PGGGSEGGSDQAPEAEYEEVKK 682

BLAST of Carg08089-RA vs. TAIR 10
Match: AT4G37910.1 (mitochondrial heat shock protein 70-1 )

HSP 1 Score: 1069.7 bits (2765), Expect = 9.7e-313
Identity = 557/681 (81.79%), Postives = 626/681 (91.92%), Query Frame = 0

Query: 1   MATGVLLRSLRRRDLASSSISAYRSLTSNAKSSLARSHLSQSWASLSRPFSSKPAGSDVI 60
           MA+  LLRS RRR++  +S+SA++S+++N K+S     +      L+RPF S+P G+DVI
Sbjct: 1   MASVALLRSFRRREVQMASVSAFKSVSANGKNS-----MFGKLGYLARPFCSRPVGNDVI 60

Query: 61  GIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPTNT 120
           GIDLGTTNSCVSVMEGK  +VIEN+EG+RTTPSVVA NQKGELLVGTPAKRQAVTNPTNT
Sbjct: 61  GIDLGTTNSCVSVMEGKTARVIENAEGSRTTPSVVAMNQKGELLVGTPAKRQAVTNPTNT 120

Query: 121 VFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVEANGQQYSPSQIGAFVLTKMKE 180
           +FG+KRLIGRRFDDPQTQKEMKMVPYKIV+APNGDAWVEANGQ++SPSQIGA VLTKMKE
Sbjct: 121 IFGSKRLIGRRFDDPQTQKEMKMVPYKIVKAPNGDAWVEANGQKFSPSQIGANVLTKMKE 180

Query: 181 TAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGMNNKE 240
           TAEAYLGKS++KAV+TVPAYFNDAQRQATKDAG+IAGLDVQRIINEPTAAALSYGMNNKE
Sbjct: 181 TAEAYLGKSINKAVVTVPAYFNDAQRQATKDAGKIAGLDVQRIINEPTAAALSYGMNNKE 240

Query: 241 GLIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKRTDGID 300
           G+IAVFDLGGGTFDVSILEIS+GVFEVK+TNGDTFLGGEDFDN LL++LV+EFKR+D ID
Sbjct: 241 GVIAVFDLGGGTFDVSILEISSGVFEVKATNGDTFLGGEDFDNTLLEYLVNEFKRSDNID 300

Query: 301 LTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFESLVSN 360
           LTKD LALQRLREAAEKAKIELSST+QTEINLPFITADASGAKHLNITLTRSKFE LV  
Sbjct: 301 LTKDNLALQRLREAAEKAKIELSSTTQTEINLPFITADASGAKHLNITLTRSKFEGLVGK 360

Query: 361 LIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVNPDEAV 420
           LIERT++PC+NCLKDA ++IKEVDEVLLVGGMTRVPKVQE+V+EIFGKSP KGVNPDEAV
Sbjct: 361 LIERTRSPCQNCLKDAGVTIKEVDEVLLVGGMTRVPKVQEIVSEIFGKSPCKGVNPDEAV 420

Query: 421 AMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVFSTAAD 480
           AMGAAIQGGILRGDVK+LLLLDV PLSLGIETLG +FT+LI RNTTIPTKKSQVFSTAAD
Sbjct: 421 AMGAAIQGGILRGDVKDLLLLDVVPLSLGIETLGAVFTKLIPRNTTIPTKKSQVFSTAAD 480

Query: 481 NQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTVSAKDK 540
           NQ QVGIKVLQGEREMA+DNK+LGEF+LVGIPPAPRGMPQ+EVTFDIDANGI TVSAKDK
Sbjct: 481 NQMQVGIKVLQGEREMAADNKVLGEFDLVGIPPAPRGMPQIEVTFDIDANGITTVSAKDK 540

Query: 541 STGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSIEKSLG 600
           +TGKEQ ITIRSSGGLSDDEI +MVKEAEL+AQKDQE+K LID+RNSADTTIYS+EKSL 
Sbjct: 541 ATGKEQNITIRSSGGLSDDEINRMVKEAELNAQKDQEKKQLIDLRNSADTTIYSVEKSLS 600

Query: 601 EYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHM---AGGSGG 660
           EYREKIP+E+A EIE AV+DLR AM+G+D+E+IKAK++ ANKAVSKIG+HM   +G SG 
Sbjct: 601 EYREKIPAEIASEIETAVSDLRTAMAGEDVEDIKAKVEAANKAVSKIGEHMSKGSGSSGS 660

Query: 661 DAASG-GSQGGEQASEAEYEE 678
           D +SG G+ G EQ  EAE+EE
Sbjct: 661 DGSSGEGTSGTEQTPEAEFEE 676

BLAST of Carg08089-RA vs. TAIR 10
Match: AT4G24280.1 (chloroplast heat shock protein 70-1 )

HSP 1 Score: 668.7 bits (1724), Expect = 5.0e-192
Identity = 356/626 (56.87%), Postives = 460/626 (73.48%), Query Frame = 0

Query: 59  VIGIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPT 118
           V+GIDLGTTNS V+ MEG  P ++ N+EG RTTPSVVA+ + G+ LVG  AKRQAV NP 
Sbjct: 80  VVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVAYTKSGDRLVGQIAKRQAVVNPE 139

Query: 119 NTVFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVE--ANGQQYSPSQIGAFVLT 178
           NT F  KR IGR+ +  +  +E K V Y++VR  N +  +E  A  +Q++  +I A VL 
Sbjct: 140 NTFFSVKRFIGRKMN--EVDEESKQVSYRVVRDENNNVKLECPAINKQFAAEEISAQVLR 199

Query: 179 KMKETAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGM 238
           K+ + A  +L   V+KAVITVPAYFND+QR ATKDAGRIAGL+V RIINEPTAA+L+YG 
Sbjct: 200 KLVDDASRFLNDKVTKAVITVPAYFNDSQRTATKDAGRIAGLEVLRIINEPTAASLAYGF 259

Query: 239 NNKEG-LIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKR 298
           + K    I VFDLGGGTFDVS+LE+ +GVFEV ST+GDT LGG+DFD  ++D+L +EFK+
Sbjct: 260 DRKANETILVFDLGGGTFDVSVLEVGDGVFEVLSTSGDTHLGGDDFDKRVVDWLAAEFKK 319

Query: 299 TDGIDLTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFE 358
            +GIDL KDK ALQRL EAAEKAKIELSS +QT ++LPFITA A G KH+  TLTR+KFE
Sbjct: 320 DEGIDLLKDKQALQRLTEAAEKAKIELSSLTQTNMSLPFITATADGPKHIETTLTRAKFE 379

Query: 359 SLVSNLIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVN 418
            L S+L++R + P +N L+DA +S K++DEV+LVGG TR+P VQE+V ++ GK P+  VN
Sbjct: 380 ELCSDLLDRVRTPVENSLRDAKLSFKDIDEVILVGGSTRIPAVQELVRKVTGKEPNVTVN 439

Query: 419 PDEAVAMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVF 478
           PDE VA+GAA+Q G+L GDV +++LLDVTPLS+G+ETLGG+ T++I RNTT+PT KS+VF
Sbjct: 440 PDEVVALGAAVQAGVLAGDVSDIVLLDVTPLSIGLETLGGVMTKIIPRNTTLPTSKSEVF 499

Query: 479 STAADNQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTV 538
           STAAD QT V I VLQGERE   DNK LG F L GIPPAPRG+PQ+EV FDIDANGI++V
Sbjct: 500 STAADGQTSVEINVLQGEREFVRDNKSLGSFRLDGIPPAPRGVPQIEVKFDIDANGILSV 559

Query: 539 SAKDKSTGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSI 598
           SA DK TGK+Q ITI  +  L  DE+++MV+EAE  A+ D+E++  ID +N AD+ +Y  
Sbjct: 560 SAVDKGTGKKQDITITGASTLPKDEVDQMVQEAERFAKDDKEKRDAIDTKNQADSVVYQT 619

Query: 599 EKSLGEYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHMAG-- 658
           EK L E  EKIP EV +++E  + +L+  +     +EIK  +   N+ V +IGQ +    
Sbjct: 620 EKQLKELGEKIPGEVKEKVEAKLQELKDKIGSGSTQEIKDAMAALNQEVMQIGQSLYNQP 679

Query: 659 GSGGDAASGGSQGGEQASEAEYEEVK 680
           G+GG  A G S GGE AS  +    K
Sbjct: 680 GAGGPGA-GPSPGGEGASSGDSSSSK 702

BLAST of Carg08089-RA vs. TAIR 10
Match: AT5G49910.1 (chloroplast heat shock protein 70-2 )

HSP 1 Score: 660.2 bits (1702), Expect = 1.8e-189
Identity = 351/631 (55.63%), Postives = 459/631 (72.74%), Query Frame = 0

Query: 59  VIGIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPT 118
           V+GIDLGTTNS V+ MEG  P ++ N+EG RTTPSVVA+ +  + LVG  AKRQAV NP 
Sbjct: 80  VVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVAYTKSKDRLVGQIAKRQAVVNPE 139

Query: 119 NTVFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWVE--ANGQQYSPSQIGAFVLT 178
           NT F  KR IGRR +  +  +E K V Y++++  NG+  ++  A G+Q++  +I A VL 
Sbjct: 140 NTFFSVKRFIGRRMN--EVAEESKQVSYRVIKDENGNVKLDCPAIGKQFAAEEISAQVLR 199

Query: 179 KMKETAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALSYGM 238
           K+ + A  +L   V+KAVITVPAYFND+QR ATKDAGRIAGL+V RIINEPTAA+L+YG 
Sbjct: 200 KLVDDASRFLNDKVTKAVITVPAYFNDSQRTATKDAGRIAGLEVLRIINEPTAASLAYGF 259

Query: 239 NNKEG-LIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVSEFKR 298
             K    I VFDLGGGTFDVS+LE+ +GVFEV ST+GDT LGG+DFD  ++D+L S FK+
Sbjct: 260 ERKSNETILVFDLGGGTFDVSVLEVGDGVFEVLSTSGDTHLGGDDFDKRVVDWLASTFKK 319

Query: 299 TDGIDLTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTRSKFE 358
            +GIDL KDK ALQRL EAAEKAKIELSS +QT ++LPFITA A G KH+  TLTR KFE
Sbjct: 320 DEGIDLLKDKQALQRLTEAAEKAKIELSSLTQTNMSLPFITATADGPKHIETTLTRGKFE 379

Query: 359 SLVSNLIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIFGKSPSKGVN 418
            L S+L++R + P +N L+DA +S K++DEV+LVGG TR+P VQ++V ++ GK P+  VN
Sbjct: 380 ELCSDLLDRVRTPVENSLRDAKLSFKDIDEVILVGGSTRIPAVQDLVRKLTGKEPNVSVN 439

Query: 419 PDEAVAMGAAIQGGILRGDVKELLLLDVTPLSLGIETLGGIFTRLISRNTTIPTKKSQVF 478
           PDE VA+GAA+Q G+L GDV +++LLDVTPLSLG+ETLGG+ T++I RNTT+PT KS+VF
Sbjct: 440 PDEVVALGAAVQAGVLSGDVSDIVLLDVTPLSLGLETLGGVMTKIIPRNTTLPTSKSEVF 499

Query: 479 STAADNQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFDIDANGIVTV 538
           STAAD QT V I VLQGERE   DNK +G F L GIPPAPRG+PQ+EV FDIDANGI++V
Sbjct: 500 STAADGQTSVEINVLQGEREFVRDNKSIGSFRLDGIPPAPRGVPQIEVKFDIDANGILSV 559

Query: 539 SAKDKSTGKEQQITIRSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIRNSADTTIYSI 598
           SA DK TGK+Q ITI  +  L  DE++ MV+EAE  A++D+E++  ID +N AD+ +Y  
Sbjct: 560 SASDKGTGKKQDITITGASTLPKDEVDTMVQEAERFAKEDKEKRDAIDTKNQADSVVYQT 619

Query: 599 EKSLGEYREKIPSEVAKEIEDAVADLRKAMSGDDIEEIKAKLDVANKAVSKIGQHM---- 658
           EK L E  EKIP  V +++E  + +L++ ++    +EIK  +   N+ V +IGQ +    
Sbjct: 620 EKQLKELGEKIPGPVKEKVEAKLQELKEKIASGSTQEIKDTMAALNQEVMQIGQSLYNQP 679

Query: 659 -AGGS----GGDAAS---------GGSQGGE 669
             GG+    GG+A+S         GG  GG+
Sbjct: 680 QPGGADSPPGGEASSSSDTSSSAKGGDNGGD 708

BLAST of Carg08089-RA vs. TAIR 10
Match: AT5G42020.1 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 563.1 bits (1450), Expect = 2.9e-160
Identity = 319/644 (49.53%), Postives = 434/644 (67.39%), Query Frame = 0

Query: 59  VIGIDLGTTNSCVSVMEGKNPKVIENSEGARTTPSVVAFNQKGELLVGTPAKRQAVTNPT 118
           VIGIDLGTT SCV V +  + ++I N +G R TPS V F    E L+G  AK QA  NP 
Sbjct: 37  VIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFTD-SERLIGEAAKNQAAVNPE 96

Query: 119 NTVFGTKRLIGRRFDDPQTQKEMKMVPYKIVRAPNGDAWV-----EANGQQYSPSQIGAF 178
            TVF  KRLIGR+F+D + QK+ K+VPY+IV   +G  ++     +   + +SP +I A 
Sbjct: 97  RTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVN-KDGKPYIQVKIKDGETKVFSPEEISAM 156

Query: 179 VLTKMKETAEAYLGKSVSKAVITVPAYFNDAQRQATKDAGRIAGLDVQRIINEPTAAALS 238
           +LTKMKETAEAYLGK +  AV+TVPAYFNDAQRQATKDAG IAGL+V RIINEPTAAA++
Sbjct: 157 ILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIA 216

Query: 239 YGMNNKEG--LIAVFDLGGGTFDVSILEISNGVFEVKSTNGDTFLGGEDFDNALLDFLVS 298
           YG++ K G   I VFDLGGGTFDVS+L I NGVFEV STNGDT LGGEDFD+ ++++ + 
Sbjct: 217 YGLDKKGGEKNILVFDLGGGTFDVSVLTIDNGVFEVLSTNGDTHLGGEDFDHRIMEYFIK 276

Query: 299 EFKRTDGIDLTKDKLALQRLREAAEKAKIELSSTSQTEINLPFITADASGAKHLNITLTR 358
             K+    D++KD  AL +LR   E+AK  LSS  Q  + +  +         L+  LTR
Sbjct: 277 LIKKKHQKDISKDNKALGKLRRECERAKRALSSQHQVRVEIESLFDGVD----LSEPLTR 336

Query: 359 SKFESLVSNLIERTKAPCKNCLKDANISIKEVDEVLLVGGMTRVPKVQEVVTEIF-GKSP 418
           ++FE L ++L  +T  P K  + DA +   ++DE++LVGG TR+PKVQ+++ + F GK P
Sbjct: 337 ARFEELNNDLFRKTMGPVKKAMDDAGLQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEP 396

Query: 419 SKGVNPDEAVAMGAAIQGGILRG----DVKELLLLDVTPLSLGIETLGGIFTRLISRNTT 478
           +KGVNPDEAVA GAA+QGGIL G    + K++LLLDV PL+LGIET+GG+ T+LI RNT 
Sbjct: 397 NKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTV 456

Query: 479 IPTKKSQVFSTAADNQTQVGIKVLQGEREMASDNKLLGEFELVGIPPAPRGMPQVEVTFD 538
           IPTKKSQVF+T  D QT V I+V +GER +  D +LLG+F+L G+PPAPRG PQ+EVTF+
Sbjct: 457 IPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRLLGKFDLTGVPPAPRGTPQIEVTFE 516

Query: 539 IDANGIVTVSAKDKSTGKEQQITI-RSSGGLSDDEIEKMVKEAELHAQKDQERKSLIDIR 598
           +DANGI+ V A+DK++GK ++ITI    G LS +EI++MVKEAE  A++D++ K  ID R
Sbjct: 517 VDANGILNVKAEDKASGKSEKITITNEKGRLSQEEIDRMVKEAEEFAEEDKKVKEKIDAR 576

Query: 599 NSADTTIYSI-----------EKSLGEYREKIPSEVAKEIE--DAVADLRKAMSGDDIEE 658
           N+ +T +Y++           +K  G+ +EKI +   + +E  D   +  K    + ++E
Sbjct: 577 NALETYVYNMKNQVSDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEYDEKLKE 636

Query: 659 IKAKLDVANKAVSKIGQHMAGGSGGDAASGGSQGGEQASEAEYE 677
           ++A   V N  ++ + Q     SGG   +GG    E+  E+  E
Sbjct: 637 VEA---VCNPIITAVYQR----SGGAPGAGGESSTEEEDESHDE 667

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030913.10.0e+00100.00hypothetical protein SDJN02_04950 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022943125.10.0e+0099.85heat shock 70 kDa protein, mitochondrial-like [Cucurbita moschata] >KAG6600255.1... [more]
XP_023515841.10.0e+0099.26heat shock 70 kDa protein, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_022999746.10.0e+0098.97heat shock 70 kDa protein, mitochondrial-like [Cucurbita maxima][more]
XP_022922655.10.0e+0096.03heat shock 70 kDa protein, mitochondrial [Cucurbita moschata] >KAG6576887.1 hypo... [more]
Match NameE-valueIdentityDescription
Q018990.0e+0090.68Heat shock 70 kDa protein, mitochondrial OS=Phaseolus vulgaris OX=3885 PE=2 SV=1[more]
P379000.0e+0090.22Heat shock 70 kDa protein, mitochondrial OS=Pisum sativum OX=3888 GN=HSP1 PE=2 S... [more]
Q9LDZ00.0e+0086.80Heat shock 70 kDa protein 10, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Q082760.0e+0083.87Heat shock 70 kDa protein, mitochondrial OS=Solanum tuberosum OX=4113 GN=HSP68 P... [more]
Q8GUM21.4e-31181.79Heat shock 70 kDa protein 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HS... [more]
Match NameE-valueIdentityDescription
A0A6J1FXE80.0e+0099.85heat shock 70 kDa protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1KDZ10.0e+0098.97heat shock 70 kDa protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1E9E10.0e+0096.03heat shock 70 kDa protein, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1JBC50.0e+0095.88heat shock 70 kDa protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1D5410.0e+0095.88heat shock 70 kDa protein, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT5G09590.10.0e+0086.80mitochondrial HSO70 2 [more]
AT4G37910.19.7e-31381.79mitochondrial heat shock protein 70-1 [more]
AT4G24280.15.0e-19256.87chloroplast heat shock protein 70-1 [more]
AT5G49910.11.8e-18955.63chloroplast heat shock protein 70-2 [more]
AT5G42020.12.9e-16049.53Heat shock protein 70 (Hsp 70) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 301..328
NoneNo IPR availablePRINTSPR00301HEATSHOCK70coord: 414..434
score: 71.81
coord: 383..399
score: 65.7
coord: 251..261
score: 76.36
coord: 109..117
score: 68.38
coord: 193..213
score: 92.75
coord: 86..98
score: 70.45
coord: 437..456
score: 76.02
coord: 58..71
score: 79.5
coord: 518..534
score: 70.82
NoneNo IPR availableGENE3D3.30.420.40coord: 235..415
e-value: 2.7E-170
score: 568.0
NoneNo IPR availableGENE3D3.90.640.10Actin; Chain A, domain 4coord: 278..365
e-value: 2.7E-170
score: 568.0
NoneNo IPR availableGENE3D3.30.420.40coord: 60..428
e-value: 2.7E-170
score: 568.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 649..680
NoneNo IPR availablePANTHERPTHR19375:SF451HEAT SHOCK 70 KDA PROTEIN, MITOCHONDRIALcoord: 30..662
NoneNo IPR availableCDDcd11733HSPA9-like_NBDcoord: 56..431
e-value: 0.0
score: 778.096
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilyGENE3D2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1coord: 431..555
e-value: 2.6E-58
score: 197.6
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilySUPERFAMILY100920Heat shock protein 70kD (HSP70), peptide-binding domaincoord: 433..589
IPR013126Heat shock protein 70 familyPFAMPF00012HSP70coord: 59..651
e-value: 3.4E-274
score: 910.6
IPR013126Heat shock protein 70 familyPANTHERPTHR19375HEAT SHOCK PROTEIN 70KDAcoord: 30..662
IPR029048Heat shock protein 70kD, C-terminal domain superfamilyGENE3D1.20.1270.10coord: 556..653
e-value: 1.3E-28
score: 101.4
IPR029048Heat shock protein 70kD, C-terminal domain superfamilySUPERFAMILY100934Heat shock protein 70kD (HSP70), C-terminal subdomaincoord: 556..662
IPR012725Chaperone DnaKTIGRFAMTIGR02350TIGR02350coord: 59..651
e-value: 4.4E-287
score: 951.2
IPR012725Chaperone DnaKHAMAPMF_00332DnaKcoord: 56..678
score: 36.057739
IPR018181Heat shock protein 70, conserved sitePROSITEPS00297HSP70_1coord: 62..69
IPR018181Heat shock protein 70, conserved sitePROSITEPS01036HSP70_3coord: 386..400
IPR018181Heat shock protein 70, conserved sitePROSITEPS00329HSP70_2coord: 245..258
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 240..431
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 59..239

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg08089Carg08089gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08089-RA:1Carg08089-RA:1exon
Carg08089-RA:2Carg08089-RA:2exon
Carg08089-RA:3Carg08089-RA:3exon
Carg08089-RA:4Carg08089-RA:4exon
Carg08089-RA:5Carg08089-RA:5exon
Carg08089-RA:6Carg08089-RA:6exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08089-RA:five_prime_utrCarg08089-RA:five_prime_utrfive_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08089-RA:cdsCarg08089-RA:cdsCDS
Carg08089-RA:cdsCarg08089-RA:cds_2CDS
Carg08089-RA:cdsCarg08089-RA:cds_3CDS
Carg08089-RA:cdsCarg08089-RA:cds_4CDS
Carg08089-RA:cdsCarg08089-RA:cds_5CDS
Carg08089-RA:cdsCarg08089-RA:cds_6CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08089-RA:three_prime_utrCarg08089-RA:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg08089-RACarg08089-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0034620 cellular response to unfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0051082 unfolded protein binding
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:0044183 protein folding chaperone