Carg07913-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07913-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Serine/threonine-protein kinase/endoribonuclease IRE1a
Location	Carg_Chr07: 1675638 .. 1677664 (+)
Sequence length	1149
RNA-Seq Expression	Carg07913-RA
Synteny	Carg07913-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTAGGTTTGGTTTTGATAGTTGTCAAATGCAGGGATATGGTAGCTGGGCTTGTGCATTTGCACGAGTTGGGGATAATTCATCGGGACTTGAAGCCACAAAATGTGTTGATAATTAAGAAAAAATCTATATGTGCAAAGCTCTCTGATATGGGAATCAGCAAGCATCTTTCAGCTGATATGTCATCCTTGGGACATCGTGCCACTGGCAAGTATTCTTCACTAGCTTTCGTCTCCCTTCAATACTCATGTTATATTCAACATGCATTCCTGGTTGATAATGAGGCTTTTTAAGTCTCTAGAAATGATGGGTAACTTGAGACAAAAATCCTATCGCAGGTTGTGGGAGTTCTGGTTGGCAAGCCCCCGAACAGCTTCTTCAGGGGCGACAAACCCGTGCAGTCGATTTGTTTAGTTTAGGTTGTGTTCTCTTTTTCTGCATCACAGGTGGTAGACATCCATTTGGTGATCGTCTCGAGCGTGATATCAATATTGTGAAGAATCAAATGAATTTGTTCTTGGTGGAAAGCATCCCAGAAGCTCTAGATCTTATTTCTCAATTGTTAAATCCTAACCCTTGTTTGAGGTAAGAAATCTCTGTTCATGAACATTAAAAAATCCTTAGATATCATTTATGTAGTGTAAAAGCATATCTACTTCATCTCTCTTGATAATATACCAGACCAAAAGCATCAGAGGTGTTGCACCATCCTTTTTTCTGGAGTTCGGAGATGCGACTTTCTTTTCTTCATGATACTAGTGATAGAATAGAGTTGGAAGACAGAGAGACTAATCTCATGAAAGCATTAGAGAATACTGCCCAGTTAGCTATAGGCACTAAATGGGATGAAAAGATGGAACCAGCTTTCATTGCTAACATCGGTTGGTATCGGCGTTACAAGTATGATAGCGTTCGAGACTTGCTGCGAGTTATGCGCAATAAACTGAATCATTATAGAGAACTGCCGAAAGAAATTCAGGTTTCCTTCCTTTTTTTTCATTCTTTGATCGTTCATTTTAATGAGCCAAAATCGGTGACAACCCATCGTTTTGTGTTTTCGTTGTCATTTCAGGAGCTCATAGGATCTGTTCCAGAGGGATTCGATGGTTACTTTGCTAGTAGGTTTCCAAGACTCTTGATTGAAGTTTACAAAGTTATAAGTCAATACTGTAGGCAAGAAGAGTGCTTTCAGAAGTACTTTAAAAGCCCTGTAGACTAGATTCTTAGATATGAATGTCTTGAAAAAAATGCTAAAATTATTATGGTAGCATGGTTAGAGTACTAATATACATGTATACATTTGTAAATATTGCTGAATAGATCTGAATTACTGTAAAGTGTTACAATACAAAGAACATTTTTGGCTCTTAAATTTCGTCCTCTCTCGATACTTGATGATACTGTTGCTAAAGAAAATCGAGTAAGCGCTAACAACATGCAACCATGATGCCTGTTTCTAACCTGTACCAGTAGTTTCACAAATGTATGTTACTGTCCACGAAGATCATATGCATCAATACATACCATCAGCATCACAATTGTCATATTTTCTTGAACTGCCTGCTGAATTATCTCCATTCATGTTTCTAACTGAAGCCTCCTGCATCTGTCGTCGCTTGGATCGACTCCTAAAGACATCTTCCTTTTCATCTGCAAAAGAAAGAAATCTATGTAGGCTTTTAGACCGAGATCGAGATCGAGAGGTTTGCTCAGAAGATTCAAGTTCTTCTGAGTAATGCTTGCTGTATTGGCGCAACATTTGAAAGACGTATATCGCTAGAGCGAGCAAGCAGACTAATCCACATATTAGAAAAAGTCCCCAAAAGCTATCGAGCTGAAGTCGATCCACCTCAAATTTGGAGACTTGTGATGTGCAAGCACTTTTCATTAACCATTTATCATGGATTCTTTGAAGATCCCCATTTTCGGACAGTCGTAGAATAGCGGTCGACATGTCGACTGCTAAAGGAGAGTCGCGCTGGAAAGCCTGTTGGTATAATGTACCATCATCAAATGACAACTAA

mRNA sequence

ATGAAGGATATGGTAGCTGGGCTTGTGCATTTGCACGAGTTGGGGATAATTCATCGGGACTTGAAGCCACAAAATGTGTTGATAATTAAGAAAAAATCTATATGTGCAAAGCTCTCTGATATGGGAATCAGCAAGCATCTTTCAGCTGATATGTCATCCTTGGGACATCGTTGTGGGAGTTCTGGTTGGCAAGCCCCCGAACAGCTTCTTCAGGGGCGACAAACCCGTGCAGTCGATTTGTTTAGTTTAGGTTGTGTTCTCTTTTTCTGCATCACAGGTGGTAGACATCCATTTGGTGATCGTCTCGAGCGTGATATCAATATTGTGAAGAATCAAATGAATTTGTTCTTGGTGGAAAGCATCCCAGAAGCTCTAGATCTTATTTCTCAATTGTTAAATCCTAACCCTTGTTTGAGACCAAAAGCATCAGAGGTGTTGCACCATCCTTTTTTCTGGAGTTCGGAGATGCGACTTTCTTTTCTTCATGATACTAGTGATAGAATAGAGTTGGAAGACAGAGAGACTAATCTCATGAAAGCATTAGAGAATACTGCCCAGTTAGCTATAGGCACTAAATGGGATGAAAAGATGGAACCAGCTTTCATTGCTAACATCGGTTGGTATCGGCGTTACAAGTATGATAGCGTTCGAGACTTGCTGCGAGTTATGCGCAATAAACTGAATCATTATAGAGAACTGCCGAAAGAAATTCAGGAGCTCATAGGATCTGTTCCAGAGGGATTCGATGGTTACTTTGCTAGTAGGTTTCCAAGACTCTTGATTGAAGTTTACAAAGTTATAAGTCAATACTGTAGGCAAGAAGAGTGCTTTCAGAAGTACTTTAAAAGCCCTACCGAGATCGAGATCGAGAGGTTTGCTCAGAAGATTCAAGTTCTTCTGAGTAATGCTTGCTGTATTGGCGCAACATTTGAAAGACGTATATCGCTAGAGCGAGCAAGCAGACTAATCCACATATTAGAAAAAGTCCCCAAAAGCTATCGAGCTGAAGTCGATCCACCTCAAATTTGGAGACTTGTGATATCCCCATTTTCGGACAGTCGTAGAATAGCGGTCGACATGTCGACTGCTAAAGGAGAGTCGCGCTGGAAAGCCTGTTGGTATAATGTACCATCATCAAATGACAACTAA

Coding sequence (CDS)

ATGAAGGATATGGTAGCTGGGCTTGTGCATTTGCACGAGTTGGGGATAATTCATCGGGACTTGAAGCCACAAAATGTGTTGATAATTAAGAAAAAATCTATATGTGCAAAGCTCTCTGATATGGGAATCAGCAAGCATCTTTCAGCTGATATGTCATCCTTGGGACATCGTTGTGGGAGTTCTGGTTGGCAAGCCCCCGAACAGCTTCTTCAGGGGCGACAAACCCGTGCAGTCGATTTGTTTAGTTTAGGTTGTGTTCTCTTTTTCTGCATCACAGGTGGTAGACATCCATTTGGTGATCGTCTCGAGCGTGATATCAATATTGTGAAGAATCAAATGAATTTGTTCTTGGTGGAAAGCATCCCAGAAGCTCTAGATCTTATTTCTCAATTGTTAAATCCTAACCCTTGTTTGAGACCAAAAGCATCAGAGGTGTTGCACCATCCTTTTTTCTGGAGTTCGGAGATGCGACTTTCTTTTCTTCATGATACTAGTGATAGAATAGAGTTGGAAGACAGAGAGACTAATCTCATGAAAGCATTAGAGAATACTGCCCAGTTAGCTATAGGCACTAAATGGGATGAAAAGATGGAACCAGCTTTCATTGCTAACATCGGTTGGTATCGGCGTTACAAGTATGATAGCGTTCGAGACTTGCTGCGAGTTATGCGCAATAAACTGAATCATTATAGAGAACTGCCGAAAGAAATTCAGGAGCTCATAGGATCTGTTCCAGAGGGATTCGATGGTTACTTTGCTAGTAGGTTTCCAAGACTCTTGATTGAAGTTTACAAAGTTATAAGTCAATACTGTAGGCAAGAAGAGTGCTTTCAGAAGTACTTTAAAAGCCCTACCGAGATCGAGATCGAGAGGTTTGCTCAGAAGATTCAAGTTCTTCTGAGTAATGCTTGCTGTATTGGCGCAACATTTGAAAGACGTATATCGCTAGAGCGAGCAAGCAGACTAATCCACATATTAGAAAAAGTCCCCAAAAGCTATCGAGCTGAAGTCGATCCACCTCAAATTTGGAGACTTGTGATATCCCCATTTTCGGACAGTCGTAGAATAGCGGTCGACATGTCGACTGCTAAAGGAGAGTCGCGCTGGAAAGCCTGTTGGTATAATGTACCATCATCAAATGACAACTAA

Protein sequence

MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRCGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFLVESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTEIEIERFAQKIQVLLSNACCIGATFERRISLERASRLIHILEKVPKSYRAEVDPPQIWRLVISPFSDSRRIAVDMSTAKGESRWKACWYNVPSSNDN

Homology

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BLAST of Carg07913-RA vs. NCBI nr
Match: KAG7026700.1 (Serine/threonine-protein kinase/endoribonuclease IRE1a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781.9 bits (2018), Expect = 2.4e-222
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRCGS 60
           MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRCGS
Sbjct: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRCGS 60

Query: 61  SGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFLVES 120
           SGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFLVES
Sbjct: 61  SGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFLVES 120

Query: 121 IPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNLMKA 180
           IPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNLMKA
Sbjct: 121 IPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNLMKA 180

Query: 181 LENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL 240
           LENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL
Sbjct: 181 LENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL 240

Query: 241 IGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTEIEIERFAQKIQVLL 300
           IGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTEIEIERFAQKIQVLL
Sbjct: 241 IGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTEIEIERFAQKIQVLL 300

Query: 301 SNACCIGATFERRISLERASRLIHILEKVPKSYRAEVDPPQIWRLVISPFSDSRRIAVDM 360
           SNACCIGATFERRISLERASRLIHILEKVPKSYRAEVDPPQIWRLVISPFSDSRRIAVDM
Sbjct: 301 SNACCIGATFERRISLERASRLIHILEKVPKSYRAEVDPPQIWRLVISPFSDSRRIAVDM 360

Query: 361 STAKGESRWKACWYNVPSSNDN 383
           STAKGESRWKACWYNVPSSNDN
Sbjct: 361 STAKGESRWKACWYNVPSSNDN 382

[Close]

BLAST of Carg07913-RA vs. NCBI nr
Match: KAG6594729.1 (Serine/threonine-protein kinase/endoribonuclease IRE1b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 578.9 bits (1491), Expect = 3.1e-161
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR   
Sbjct: 626 MRDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRATG 685

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 686 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 745

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 746 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 805

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 806 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 865

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 866 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 914

[Close]

BLAST of Carg07913-RA vs. NCBI nr
Match: XP_022926590.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 578.9 bits (1491), Expect = 3.1e-161
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR   
Sbjct: 498 MRDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRATG 557

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 558 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 617

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 618 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 677

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 678 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 737

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 738 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 786

[Close]

BLAST of Carg07913-RA vs. NCBI nr
Match: XP_022926589.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 578.9 bits (1491), Expect = 3.1e-161
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR   
Sbjct: 542 MRDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRATG 601

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 602 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 661

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 662 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 721

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 722 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 781

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 782 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 830

[Close]

BLAST of Carg07913-RA vs. NCBI nr
Match: XP_022926588.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 578.9 bits (1491), Expect = 3.1e-161
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR   
Sbjct: 626 MRDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRATG 685

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 686 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 745

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 746 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 805

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 806 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 865

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 866 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 914

[Close]

BLAST of Carg07913-RA vs. ExPASy Swiss-Prot
Match: Q9C5S2 (Serine/threonine-protein kinase/endoribonuclease IRE1a OS=Arabidopsis thaliana OX=3702 GN=IRE1A PE=1 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 6.6e-122
Identity = 212/287 (73.87%), Postives = 251/287 (87.46%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRC-- 60
           M+D+V G+VHLHELGI+HRDLKPQNVLI K  ++ AKLSDMGISK +S DMSSLGH    
Sbjct: 551 MRDIVCGIVHLHELGIVHRDLKPQNVLISKDMTLSAKLSDMGISKRMSRDMSSLGHLATG 610

Query: 61  -GSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
            GSSGWQAPEQLLQGRQTRAVD+FSLGCV+F+ ITG +HPFGD LERD+NIVKN+++LFL
Sbjct: 611 SGSSGWQAPEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPFGDDLERDVNIVKNKVDLFL 670

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRE--T 180
           VE +PEA DLIS+LLNP+P LRP A+EVL HP FW+SEMRLSFL D SDR+ELE+RE  +
Sbjct: 671 VEHVPEASDLISRLLNPDPDLRPSATEVLLHPMFWNSEMRLSFLRDASDRVELENREADS 730

Query: 181 NLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPK 240
            ++KA+E+TA +AIG KWDEK+EP FI NIG YRRYKYDS+RDLLRV+RNKLNH+RELP 
Sbjct: 731 EILKAMESTAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSIRDLLRVIRNKLNHHRELPP 790

Query: 241 EIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFK 283
           EIQEL+G+VPEGFD YFA RFP+LLIEVY+VIS +CR+EE F+KYFK
Sbjct: 791 EIQELVGTVPEGFDEYFAVRFPKLLIEVYRVISLHCREEEVFRKYFK 837

[Close]

BLAST of Carg07913-RA vs. ExPASy Swiss-Prot
Match: Q93VJ2 (Serine/threonine-protein kinase/endoribonuclease IRE1b OS=Arabidopsis thaliana OX=3702 GN=IRE1B PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.1e-113
Identity = 193/290 (66.55%), Postives = 242/290 (83.45%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSL-----G 60
           M+D+VAGLVHLH++GI+HRDLKPQNVLI+K  S+CAKLSDMGISK L AD S+L     G
Sbjct: 589 MRDIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG 648

Query: 61  HRCGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNL 120
              GSSGWQAPEQL   RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERD+N++ +Q +L
Sbjct: 649 LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDL 708

Query: 121 FLVESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRE- 180
           FL+ES+PEA+ L++ LLNP+P LRP+A +V+HHP FW+S+MRLSFL D SDR+ELE+RE 
Sbjct: 709 FLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREE 768

Query: 181 -TNLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYREL 240
            + L+ ALE+TA + +  +WDEK++  F+ NIG YRRYK+DS+RDLLRV+RNKLNHYREL
Sbjct: 769 GSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYREL 828

Query: 241 PKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKS 284
           PKE+QEL+GSVPEGF+ YF+SRFP+LLI+VY V+  YC  EE F KY K+
Sbjct: 829 PKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKT 878

[Close]

BLAST of Carg07913-RA vs. ExPASy Swiss-Prot
Match: Q7XIT1 (Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Oryza sativa subsp. japonica OX=39947 GN=IRE1 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.6e-99
Identity = 175/284 (61.62%), Postives = 224/284 (78.87%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+D+VAG+VHLH LGIIHRDLKPQNVLI K+  + AKLSDMGISK L  DM+S+ H    
Sbjct: 606 MRDVVAGIVHLHSLGIIHRDLKPQNVLISKEGPLRAKLSDMGISKRLQEDMTSVSHHGTG 665

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
            GSSGWQAPEQL  GRQTRA+DLFSLGC++F+CIT G+HPFG+  ERD+ I+ NQ +LF+
Sbjct: 666 FGSSGWQAPEQLRHGRQTRAIDLFSLGCLIFYCITKGKHPFGEYYERDMKIINNQFDLFI 725

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           V+ IPEA+ LISQLL+P+P  RP A  V+HHPFFWS E+ LSFL DTSDRIE +  ET+L
Sbjct: 726 VDHIPEAVHLISQLLDPDPEKRPTAVYVMHHPFFWSPELCLSFLRDTSDRIE-KTSETDL 785

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           + ALE     A G  W EK++ A +A++G YR+Y ++S RDLLR++RNK  HYRE   ++
Sbjct: 786 IDALEGINVEAFGKNWGEKLDAALLADMGRYRKYSFESTRDLLRLIRNKSGHYREFSDDL 845

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYF 282
           +EL+GS+PEGF  YF+SRFP+LLI+VY+V+S++C+ EE F KYF
Sbjct: 846 KELLGSLPEGFVQYFSSRFPKLLIKVYEVMSEHCKDEEAFSKYF 888

[Close]

BLAST of Carg07913-RA vs. ExPASy Swiss-Prot
Match: Q9SF12 (Inactive serine/threonine-protein kinase/endoribonuclease IRE1-like OS=Arabidopsis thaliana OX=3702 GN=At3g11870 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.1e-63
Identity = 140/304 (46.05%), Postives = 188/304 (61.84%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLII-KKKSICAKLSDMGISKHLSADMSSLGHRC- 60
           M+D+V GL HLH+L  IHR+L PQNVLII K  ++ AK+SDM +SKHL    SS  H   
Sbjct: 252 MRDVVCGLAHLHKLKTIHRNLNPQNVLIIVKDMTLTAKISDMSLSKHLGGKKSSYKHLAT 311

Query: 61  --GSSGWQAPEQLLQGRQTR---AVDLFSLGCVLFFCITGGRHPFGDRLERDINI-VKNQ 120
             GSSGWQAPEQL + ++ +     D+F+ GC+L + +  G HPFG   ERD NI   N+
Sbjct: 312 CSGSSGWQAPEQLNKDKKKKEDFPADMFNFGCLLHYAVM-GTHPFGSPSERDTNIKTNNK 371

Query: 121 MNLFLVESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELED 180
            NL LV ++ EA++LI QLLN  P LRP A++VL HP FW SE RL FL + SDRIEL+ 
Sbjct: 372 TNLSLVTNL-EAINLIEQLLNYKPDLRPSATQVLLHPLFWDSEKRLFFLREASDRIELDI 431

Query: 181 RETNLMKALENTAQLAIGTKWDEKMEPAFIANI-------------GWYRRYKYDSVRDL 240
                +        L     W  K+   FI +I              + R YKY S+R L
Sbjct: 432 TMWGDLNKTIAPRVLGESKDWASKLGKTFITHIENLAQAQPGQESRQYNRSYKYWSLRHL 491

Query: 241 LRVMRNKLNHYREL--PKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECF 282
           LR++RN L+H+RE+    +I+E++G VPEG D +F +RFP L++E+Y  IS +C+ EE F
Sbjct: 492 LRLIRNILSHHREILDDPKIKEMVGKVPEGLDIFFTARFPNLMMEIYAFISMHCKGEEAF 551

[Close]

BLAST of Carg07913-RA vs. ExPASy Swiss-Prot
Match: Q09499 (Serine/threonine-protein kinase/endoribonuclease ire-1 OS=Caenorhabditis elegans OX=6239 GN=ire-1 PE=1 SV=2)

HSP 1 Score: 243.4 bits (620), Expect = 4.1e-63
Identity = 130/320 (40.62%), Postives = 190/320 (59.38%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLII---KKKSICAKLSDMGISKHLSADMSSLGH- 60
           MK    GL HLH   I+HRD+KPQNVLI    ++  + A +SD G+ K +    +S+   
Sbjct: 617 MKQATDGLAHLHASKIVHRDMKPQNVLITMASQRGEMRAVISDFGLCKRVQPGKNSISRG 676

Query: 61  ----RCGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQ 120
                 G+ GW APE L+    +  VD+FSLGC+ ++ +T G HPFG  L R  NIV  +
Sbjct: 677 IASGLAGTDGWIAPEVLISASTSYPVDIFSLGCIFYYVLTSGTHPFGKSLHRQANIVNGE 736

Query: 121 MNLFLVESIPE---ALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIE 180
             L  +  + +   A DLIS +LN  P  R  A  VL+HPFFW+SE RL++  D SDR+E
Sbjct: 737 YTLNKLADLDDWSLADDLISSMLNVEPLHRLTADAVLNHPFFWTSEKRLAYFSDVSDRVE 796

Query: 181 LEDRETNLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNH 240
            E+  + +++ +E  A++ +   W EK+  A   ++  +R YK  SVRDLLR MRNK +H
Sbjct: 797 KEEDNSPVVRRIETDARIVVCGGWREKICDALKEDLRKFRTYKSFSVRDLLRAMRNKKHH 856

Query: 241 YRELPKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKS------ 300
           YRELP+++++ +G +P+ F  YF SRFPRLL+ VYK  ++YC  E  F++Y+        
Sbjct: 857 YRELPEDVRQSLGDIPDQFLHYFTSRFPRLLLHVYKA-TEYCSGEAVFKRYYSDDVRARM 916

Query: 301 -PTEIEIERFAQKIQVLLSN 303
            P   E ER  +KI+  ++N
Sbjct: 917 YPIVEEEERVRKKIKEEMAN 935

[Close]

BLAST of Carg07913-RA vs. ExPASy TrEMBL
Match: A0A6J1ELJ4 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433677 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR   
Sbjct: 498 MRDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRATG 557

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 558 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 617

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 618 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 677

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 678 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 737

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 738 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 786

[Close]

BLAST of Carg07913-RA vs. ExPASy TrEMBL
Match: A0A6J1EIH5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111433677 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR   
Sbjct: 626 MRDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRATG 685

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 686 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 745

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 746 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 805

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 806 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 865

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 866 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 914

[Close]

BLAST of Carg07913-RA vs. ExPASy TrEMBL
Match: A0A6J1EFL5 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433677 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 283/289 (97.92%), Postives = 285/289 (98.62%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR   
Sbjct: 542 MRDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRATG 601

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 602 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 661

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 662 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 721

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 722 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 781

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 782 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 830

[Close]

BLAST of Carg07913-RA vs. ExPASy TrEMBL
Match: A0A6J1KQM6 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496759 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.2e-160
Identity = 281/289 (97.23%), Postives = 284/289 (98.27%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGL HLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGIS+HLSADMSSLGHR   
Sbjct: 498 MRDMVAGLEHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISQHLSADMSSLGHRATG 557

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 558 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 617

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 618 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 677

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 678 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 737

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 738 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 786

[Close]

BLAST of Carg07913-RA vs. ExPASy TrEMBL
Match: A0A6J1KS56 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496759 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.2e-160
Identity = 281/289 (97.23%), Postives = 284/289 (98.27%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHR--- 60
           M+DMVAGL HLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGIS+HLSADMSSLGHR   
Sbjct: 542 MRDMVAGLEHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISQHLSADMSSLGHRATG 601

Query: 61  CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
           CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL
Sbjct: 602 CGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 661

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 180
           VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL
Sbjct: 662 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRETNL 721

Query: 181 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 240
           MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI
Sbjct: 722 MKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPKEI 781

Query: 241 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPTE 287
           QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSP +
Sbjct: 782 QELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKSPVD 830

[Close]

BLAST of Carg07913-RA vs. TAIR 10
Match: AT2G17520.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 438.7 bits (1127), Expect = 4.7e-123
Identity = 212/287 (73.87%), Postives = 251/287 (87.46%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSLGHRC-- 60
           M+D+V G+VHLHELGI+HRDLKPQNVLI K  ++ AKLSDMGISK +S DMSSLGH    
Sbjct: 551 MRDIVCGIVHLHELGIVHRDLKPQNVLISKDMTLSAKLSDMGISKRMSRDMSSLGHLATG 610

Query: 61  -GSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNLFL 120
            GSSGWQAPEQLLQGRQTRAVD+FSLGCV+F+ ITG +HPFGD LERD+NIVKN+++LFL
Sbjct: 611 SGSSGWQAPEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPFGDDLERDVNIVKNKVDLFL 670

Query: 121 VESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRE--T 180
           VE +PEA DLIS+LLNP+P LRP A+EVL HP FW+SEMRLSFL D SDR+ELE+RE  +
Sbjct: 671 VEHVPEASDLISRLLNPDPDLRPSATEVLLHPMFWNSEMRLSFLRDASDRVELENREADS 730

Query: 181 NLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYRELPK 240
            ++KA+E+TA +AIG KWDEK+EP FI NIG YRRYKYDS+RDLLRV+RNKLNH+RELP 
Sbjct: 731 EILKAMESTAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSIRDLLRVIRNKLNHHRELPP 790

Query: 241 EIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFK 283
           EIQEL+G+VPEGFD YFA RFP+LLIEVY+VIS +CR+EE F+KYFK
Sbjct: 791 EIQELVGTVPEGFDEYFAVRFPKLLIEVYRVISLHCREEEVFRKYFK 837

[Close]

BLAST of Carg07913-RA vs. TAIR 10
Match: AT5G24360.1 (inositol requiring 1-1 )

HSP 1 Score: 411.4 bits (1056), Expect = 8.0e-115
Identity = 193/290 (66.55%), Postives = 242/290 (83.45%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSL-----G 60
           M+D+VAGLVHLH++GI+HRDLKPQNVLI+K  S+CAKLSDMGISK L AD S+L     G
Sbjct: 589 MRDIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG 648

Query: 61  HRCGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNL 120
              GSSGWQAPEQL   RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERD+N++ +Q +L
Sbjct: 649 LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDL 708

Query: 121 FLVESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRE- 180
           FL+ES+PEA+ L++ LLNP+P LRP+A +V+HHP FW+S+MRLSFL D SDR+ELE+RE 
Sbjct: 709 FLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREE 768

Query: 181 -TNLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYREL 240
            + L+ ALE+TA + +  +WDEK++  F+ NIG YRRYK+DS+RDLLRV+RNKLNHYREL
Sbjct: 769 GSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYREL 828

Query: 241 PKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKS 284
           PKE+QEL+GSVPEGF+ YF+SRFP+LLI+VY V+  YC  EE F KY K+
Sbjct: 829 PKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKT 878

[Close]

BLAST of Carg07913-RA vs. TAIR 10
Match: AT5G24360.2 (inositol requiring 1-1 )

HSP 1 Score: 411.4 bits (1056), Expect = 8.0e-115
Identity = 193/290 (66.55%), Postives = 242/290 (83.45%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSL-----G 60
           M+D+VAGLVHLH++GI+HRDLKPQNVLI+K  S+CAKLSDMGISK L AD S+L     G
Sbjct: 595 MRDIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG 654

Query: 61  HRCGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNL 120
              GSSGWQAPEQL   RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERD+N++ +Q +L
Sbjct: 655 LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDL 714

Query: 121 FLVESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRE- 180
           FL+ES+PEA+ L++ LLNP+P LRP+A +V+HHP FW+S+MRLSFL D SDR+ELE+RE 
Sbjct: 715 FLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREE 774

Query: 181 -TNLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYREL 240
            + L+ ALE+TA + +  +WDEK++  F+ NIG YRRYK+DS+RDLLRV+RNKLNHYREL
Sbjct: 775 GSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYREL 834

Query: 241 PKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKS 284
           PKE+QEL+GSVPEGF+ YF+SRFP+LLI+VY V+  YC  EE F KY K+
Sbjct: 835 PKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKT 884

[Close]

BLAST of Carg07913-RA vs. TAIR 10
Match: AT5G24360.3 (inositol requiring 1-1 )

HSP 1 Score: 411.4 bits (1056), Expect = 8.0e-115
Identity = 193/290 (66.55%), Postives = 242/290 (83.45%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLIIKKKSICAKLSDMGISKHLSADMSSL-----G 60
           M+D+VAGLVHLH++GI+HRDLKPQNVLI+K  S+CAKLSDMGISK L AD S+L     G
Sbjct: 575 MRDIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG 634

Query: 61  HRCGSSGWQAPEQLLQGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDINIVKNQMNL 120
              GSSGWQAPEQL   RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERD+N++ +Q +L
Sbjct: 635 LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDL 694

Query: 121 FLVESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELEDRE- 180
           FL+ES+PEA+ L++ LLNP+P LRP+A +V+HHP FW+S+MRLSFL D SDR+ELE+RE 
Sbjct: 695 FLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREE 754

Query: 181 -TNLMKALENTAQLAIGTKWDEKMEPAFIANIGWYRRYKYDSVRDLLRVMRNKLNHYREL 240
            + L+ ALE+TA + +  +WDEK++  F+ NIG YRRYK+DS+RDLLRV+RNKLNHYREL
Sbjct: 755 GSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYREL 814

Query: 241 PKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECFQKYFKS 284
           PKE+QEL+GSVPEGF+ YF+SRFP+LLI+VY V+  YC  EE F KY K+
Sbjct: 815 PKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKT 864

[Close]

BLAST of Carg07913-RA vs. TAIR 10
Match: AT3G11870.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 245.4 bits (625), Expect = 7.6e-65
Identity = 140/304 (46.05%), Postives = 188/304 (61.84%), Query Frame = 0


Query: 1   MKDMVAGLVHLHELGIIHRDLKPQNVLII-KKKSICAKLSDMGISKHLSADMSSLGHRC- 60
           M+D+V GL HLH+L  IHR+L PQNVLII K  ++ AK+SDM +SKHL    SS  H   
Sbjct: 252 MRDVVCGLAHLHKLKTIHRNLNPQNVLIIVKDMTLTAKISDMSLSKHLGGKKSSYKHLAT 311

Query: 61  --GSSGWQAPEQLLQGRQTR---AVDLFSLGCVLFFCITGGRHPFGDRLERDINI-VKNQ 120
             GSSGWQAPEQL + ++ +     D+F+ GC+L + +  G HPFG   ERD NI   N+
Sbjct: 312 CSGSSGWQAPEQLNKDKKKKEDFPADMFNFGCLLHYAVM-GTHPFGSPSERDTNIKTNNK 371

Query: 121 MNLFLVESIPEALDLISQLLNPNPCLRPKASEVLHHPFFWSSEMRLSFLHDTSDRIELED 180
            NL LV ++ EA++LI QLLN  P LRP A++VL HP FW SE RL FL + SDRIEL+ 
Sbjct: 372 TNLSLVTNL-EAINLIEQLLNYKPDLRPSATQVLLHPLFWDSEKRLFFLREASDRIELDI 431

Query: 181 RETNLMKALENTAQLAIGTKWDEKMEPAFIANI-------------GWYRRYKYDSVRDL 240
                +        L     W  K+   FI +I              + R YKY S+R L
Sbjct: 432 TMWGDLNKTIAPRVLGESKDWASKLGKTFITHIENLAQAQPGQESRQYNRSYKYWSLRHL 491

Query: 241 LRVMRNKLNHYREL--PKEIQELIGSVPEGFDGYFASRFPRLLIEVYKVISQYCRQEECF 282
           LR++RN L+H+RE+    +I+E++G VPEG D +F +RFP L++E+Y  IS +C+ EE F
Sbjct: 492 LRLIRNILSHHREILDDPKIKEMVGKVPEGLDIFFTARFPNLMMEIYAFISMHCKGEEAF 551

The following BLAST results are available for this feature:

BLAST of Carg07913-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026700.1	2.4e-222	100.00	Serine/threonine-protein kinase/endoribonuclease IRE1a, partial [Cucurbita argyr...	[more]
KAG6594729.1	3.1e-161	97.92	Serine/threonine-protein kinase/endoribonuclease IRE1b, partial [Cucurbita argyr...	[more]
XP_022926590.1	3.1e-161	97.92	serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 [Cucurbit...	[more]
XP_022926589.1	3.1e-161	97.92	serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 [Cucurbit...	[more]
XP_022926588.1	3.1e-161	97.92	serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucurbit...	[more]

BLAST of Carg07913-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C5S2	6.6e-122	73.87	Serine/threonine-protein kinase/endoribonuclease IRE1a OS=Arabidopsis thaliana O...	[more]
Q93VJ2	1.1e-113	66.55	Serine/threonine-protein kinase/endoribonuclease IRE1b OS=Arabidopsis thaliana O...	[more]
Q7XIT1	1.6e-99	61.62	Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Oryza sativa subsp. jap...	[more]
Q9SF12	1.1e-63	46.05	Inactive serine/threonine-protein kinase/endoribonuclease IRE1-like OS=Arabidops...	[more]
Q09499	4.1e-63	40.63	Serine/threonine-protein kinase/endoribonuclease ire-1 OS=Caenorhabditis elegans...	[more]

BLAST of Carg07913-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ELJ4	1.5e-161	97.92	serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 OS=Cucurb...	[more]
A0A6J1EIH5	1.5e-161	97.92	Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1EFL5	1.5e-161	97.92	serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurb...	[more]
A0A6J1KQM6	2.2e-160	97.23	serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 OS=Cucurb...	[more]
A0A6J1KS56	2.2e-160	97.23	serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Cucurb...	[more]

BLAST of Carg07913-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G17520.1	4.7e-123	73.87	Endoribonuclease/protein kinase IRE1-like	[more]
AT5G24360.1	8.0e-115	66.55	inositol requiring 1-1	[more]
AT5G24360.2	8.0e-115	66.55	inositol requiring 1-1	[more]
AT5G24360.3	8.0e-115	66.55	inositol requiring 1-1	[more]
AT3G11870.1	7.6e-65	46.05	Endoribonuclease/protein kinase IRE1-like	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..151 e-value: 1.8E-9 score: 0.8
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 1..151 e-value: 8.6E-31 score: 107.3
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..151 score: 29.601555
None	No IPR available	SMART	SM00580	PGNneu	coord: 214..268 e-value: 8.7E-24 score: 95.0
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..153 e-value: 3.1E-44 score: 153.0
None	No IPR available	PANTHER	PTHR13954:SF27	SERINE/THREONINE-PROTEIN KINASE/ENDORIBONUCLEASE IRE1B	coord: 1..282
None	No IPR available	CDD	cd10422	RNase_Ire1	coord: 155..281 e-value: 4.95988E-64 score: 198.578
IPR038357	KEN domain superfamily	GENE3D	1.20.1440.180	KEN domain	coord: 154..294 e-value: 3.3E-50 score: 171.7
IPR010513	KEN domain	PFAM	PF06479	Ribonuc_2-5A	coord: 157..281 e-value: 1.3E-43 score: 148.2
IPR010513	KEN domain	PROSITE	PS51392	KEN	coord: 154..283 score: 37.67017
IPR045133	Serine/threonine-protein kinase/endoribonuclease IRE1/2-like	PANTHER	PTHR13954	IRE1-RELATED	coord: 1..282
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 16..28
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..168

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg07913	Carg07913	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg07913-RA:1	Carg07913-RA:1	exon
Carg07913-RA:2	Carg07913-RA:2	exon
Carg07913-RA:3	Carg07913-RA:3	exon
Carg07913-RA:4	Carg07913-RA:4	exon
Carg07913-RA:5	Carg07913-RA:5	exon
Carg07913-RA:6	Carg07913-RA:6	exon
Carg07913-RA:7	Carg07913-RA:7	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg07913-RA:cds	Carg07913-RA:cds	CDS
Carg07913-RA:cds	Carg07913-RA:cds_2	CDS
Carg07913-RA:cds	Carg07913-RA:cds_3	CDS
Carg07913-RA:cds	Carg07913-RA:cds_4	CDS
Carg07913-RA:cds	Carg07913-RA:cds_5	CDS
Carg07913-RA:cds	Carg07913-RA:cds_6	CDS
Carg07913-RA:cds	Carg07913-RA:cds_7	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg07913-RA	Carg07913-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030968	endoplasmic reticulum unfolded protein response
biological_process	GO:0006397	mRNA processing
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004540	ribonuclease activity
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity