Carg06367-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06367-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	SWIB domain-containing protein
Location	Carg_Chr06: 7572761 .. 7575215 (+)
Sequence length	634
RNA-Seq Expression	Carg06367-RA
Synteny	Carg06367-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATCTCTGTAGAAATTGATCAATTTGATTTTGTCTAATATCTTGCTGCCAGTGGAACTAAAGAATCGGCGAGTTTCTCTTAAACCTCCGCCGGGAATCGACTGCAACATCCAAGCAAGCTACAATGTCGTCTGTATTCAGCAATGGCATTAGGGTTTTCAGAGGCTGTAGAGCTCTATTAGCTCCGTCGAAATCATCGGCTGCCGTTCCCGCCGCCATCAAATCGAAGGAGAAGGCTCCTCCGAGCTCCGATGATGGTGTTTCTGCAGCCGCGCCCAAGCCGAAAATCAAAAGGGTAACGACTCGATCGACGGGGATTCTCAAGGTCGTCGAGGTTTCTCCAGCGTTGAGCAACTTCTTAGGTGTATCGGAGGTTTCTCGCACCAACGCCGTCAAGCAGATCTGGTCCTACATCAAGCTCAACAACCTTCAGGTTTCTACATTCCTTCCACTGCTATCTATGTATTTGATTGCGTACGATACCATTGTAGTTTCTAGGGAAATGAGTTCTTATCTGCGATTTTTGCTGAATGGCTTGATCGGCCAATTGATTTAGCGTGAATGTGAAGAGAAATTTCATCTTCAAGTGAACAAACTAGTTCCCCGAGCCTCCAATTTTCCTTCCCTTACACTGCAAATAAATTTGGATGTTCGTAGCAATTTCTTAGTAATGGCATCTCTCAAGTCTTAATTGATGTTCTTACCTCGAAAAATAGATGAAAAGTTCATGGGGAAGTACTTCATTACAATGTTGGAGTTTATCAAAATATTCTAAAGGCCAATTGTTCTTGCATTTACACTGGTAAATTCCCTATGAAGCTGTTTTGAATGAAATCTTCCGTTATTCTTTCTTTTCTTATAGCGATAATGATCAAAATCCATGAGTTATCTTGAAACATTGGAATTGAAAATCTTTCAGTGTCCATTGATGGACTTTTGCCTGCATTACACAAAGCTTTTATACATCTAGGTCTACCCACTCTCTCTTTCAATGAGAATACTTAAGATTGAAACGTTTGATTAAAGGATTGTATAGCATAAACACTTAGATGTGAATCCCTGCTGTTTTTTGTTCCATTTCTTGCGTCTATGAGAAACGATGTGGCATTAAAATAATAGTAGGGTAGATATGGTTAGAAACCACGACCCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAACTCTCATGACTTTGCTTTTGGTTTCCCAAAAAGGCCTCATACCATTTTTGATGTATTCCTTACTTATAAACCCATGATCATCCCATTAATTAGCCAACGTGGGACTCCTCTCAACAATCCTCCGCTCAAACAAAGTACACCATAGAGCCTCCCTTTGATCTATGGAGCCCTCGAACAACCTCACCTTAGTCGAGGCTCAACTCCTTCTCTGGTTTCGCCAAAAAGGCCTCGTACCGTCTTAGGAACCACAACCCTCCAAAATAGTATGATATTGTCTACTTTGAGCATAAGATCTCATGGCTTCCCTTTTGGTTTCCCCGAAAGGTTACTTATAAACTCATGGTCATCCTTTTAATTAGCCAATGTAGGACTCCTCCCAACAATTCTCAATAGACACTACAACCTAAGGGCATGGAAAGCTGAAGGAAAGCTTTCGATATGTTAACGATTGTAGAAAAGGTTCCACCTACCTTAGAATTTAATACCCTACGAATTTCATTGGCAACCAAATGTAATAGGGTCAAGTGGTTATCTCATGAGATTAGTCATGGCGTGTGCAAGCTTACTCAGCTATTCATGGATATCTAAAAAAAAGAACGGTAGAAAGGTTGTAATTATACAAGAATGTCAACTATAACTCCACCAATAGTAAGCTGTACATTTCTCCAAAGACTCTAAAAAGAGGAGGACTTCTCTTCGAAGGTCCTGATCTGATTCCTTCCCGCCATAACAATGTCGGAGCTGCACAACTCCGCTAAAGTTTCCTTAATGGACACCTTCCATGCTGAAAAAACACGACTCTCGTCTTTTGTAACGACAGTTAAGCCAGGGTTAACATAAGCTTGAAGATATCAAATACCATATAAGTTTCTTCCATATGACATATTGTAGGTTAGGGTCGTCTGGTTGAAGGTTTTGTTCAGCTACACGCTAAGTACCCATAGTTTTAAAAGAAAAAACAAACGGGCTAGGCCTCAAGAGCTCTGTCTTCCATGTTTAGTTGCTAATCAGGGAGTTTCTGTTCTCCAAGCTATGGAATGATGAGAATTTCATTGTCTTCCCCTGCAGAATCCTGCTAACAAAAGAGAGATCTTCTGCGACGATAAGCTTAAAGCCATATTCGAAGGAAGGGAAAAGGTCGGTTTCCTCGAGATCGCGAAATTGCTCACTCACCATTTTGTGAAGGACTGATCAGTACAGAAGATGAGAGATCAACTTCCAGACCATTTTGTGGCTCTTTATCTTGCAGCCTCAACTCTTATAGTTTTG

mRNA sequence

AAATCTCTGTAGAAATTGATCAATTTGATTTTGTCTAATATCTTGCTGCCAGTGGAACTAAAGAATCGGCGAGTTTCTCTTAAACCTCCGCCGGGAATCGACTGCAACATCCAAGCAAGCTACAATGTCGTCTGTATTCAGCAATGGCATTAGGGTTTTCAGAGGCTGTAGAGCTCTATTAGCTCCGTCGAAATCATCGGCTGCCGTTCCCGCCGCCATCAAATCGAAGGAGAAGGCTCCTCCGAGCTCCGATGATGGTGTTTCTGCAGCCGCGCCCAAGCCGAAAATCAAAAGGGTAACGACTCGATCGACGGGGATTCTCAAGGTCGTCGAGGTTTCTCCAGCGTTGAGCAACTTCTTAGGTGTATCGGAGGTTTCTCGCACCAACGCCGTCAAGCAGATCTGGTCCTACATCAAGCTCAACAACCTTCAGAATCCTGCTAACAAAAGAGAGATCTTCTGCGACGATAAGCTTAAAGCCATATTCGAAGGAAGGGAAAAGGTCGGTTTCCTCGAGATCGCGAAATTGCTCACTCACCATTTTGTGAAGGACTGATCAGTACAGAAGATGAGAGATCAACTTCCAGACCATTTTGTGGCTCTTTATCTTGCAGCCTCAACTCTTATAGTTTTG

Coding sequence (CDS)

ATGTCGTCTGTATTCAGCAATGGCATTAGGGTTTTCAGAGGCTGTAGAGCTCTATTAGCTCCGTCGAAATCATCGGCTGCCGTTCCCGCCGCCATCAAATCGAAGGAGAAGGCTCCTCCGAGCTCCGATGATGGTGTTTCTGCAGCCGCGCCCAAGCCGAAAATCAAAAGGGTAACGACTCGATCGACGGGGATTCTCAAGGTCGTCGAGGTTTCTCCAGCGTTGAGCAACTTCTTAGGTGTATCGGAGGTTTCTCGCACCAACGCCGTCAAGCAGATCTGGTCCTACATCAAGCTCAACAACCTTCAGAATCCTGCTAACAAAAGAGAGATCTTCTGCGACGATAAGCTTAAAGCCATATTCGAAGGAAGGGAAAAGGTCGGTTTCCTCGAGATCGCGAAATTGCTCACTCACCATTTTGTGAAGGACTGA

Protein sequence

MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAIFEGREKVGFLEIAKLLTHHFVKD

Homology

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BLAST of Carg06367-RA vs. NCBI nr
Match: KAG7028683.1 (spp27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.5 bits (698), Expect = 1.1e-69
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT 60
           MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT
Sbjct: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT 60

Query: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120
           RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI
Sbjct: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120

Query: 121 FEGREKVGFLEIAKLLTHHFVKD 144
           FEGREKVGFLEIAKLLTHHFVKD
Sbjct: 121 FEGREKVGFLEIAKLLTHHFVKD 143

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BLAST of Carg06367-RA vs. NCBI nr
Match: XP_022950651.1 (upstream activation factor subunit UAF30-like [Cucurbita moschata] >XP_022950661.1 upstream activation factor subunit UAF30-like [Cucurbita moschata])

HSP 1 Score: 267.3 bits (682), Expect = 7.5e-68
Identity = 141/143 (98.60%), Postives = 141/143 (98.60%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT 60
           MSS FSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDD VSAAAPKPKIKRVTT
Sbjct: 1   MSSGFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDVVSAAAPKPKIKRVTT 60

Query: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120
           RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI
Sbjct: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120

Query: 121 FEGREKVGFLEIAKLLTHHFVKD 144
           FEGREKVGFLEIAKLLTHHFVKD
Sbjct: 121 FEGREKVGFLEIAKLLTHHFVKD 143

[Close]

BLAST of Carg06367-RA vs. NCBI nr
Match: XP_023538991.1 (upstream activation factor subunit UAF30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263.5 bits (672), Expect = 1.1e-66
Identity = 139/143 (97.20%), Postives = 139/143 (97.20%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT 60
           MS  FSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDD VSAA PKPKIKRVTT
Sbjct: 1   MSFGFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDVVSAAVPKPKIKRVTT 60

Query: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120
           RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI
Sbjct: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120

Query: 121 FEGREKVGFLEIAKLLTHHFVKD 144
           FEGREKVGFLEIAKLLTHHFVKD
Sbjct: 121 FEGREKVGFLEIAKLLTHHFVKD 143

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BLAST of Carg06367-RA vs. NCBI nr
Match: XP_022974194.1 (protein TRI1-like [Cucurbita maxima] >XP_022975509.1 protein TRI1-like [Cucurbita maxima])

HSP 1 Score: 248.1 bits (632), Expect = 4.7e-62
Identity = 134/149 (89.93%), Postives = 136/149 (91.28%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAI------KSKEKAPPSSDDGVSAAAPKPK 60
           MSS FSNGIRVFRGC ALLAPSKSSAAVPA+I      KSKEKAPPSSDD VSAAAPKP 
Sbjct: 1   MSSGFSNGIRVFRGCTALLAPSKSSAAVPASIKSKATSKSKEKAPPSSDDVVSAAAPKPT 60

Query: 61  IKRVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCD 120
           IKR TTR TGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCD
Sbjct: 61  IKRATTRLTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCD 120

Query: 121 DKLKAIFEGREKVGFLEIAKLLTHHFVKD 144
           DKLKAIFEGREKVGFLEIAK L+HHFVKD
Sbjct: 121 DKLKAIFEGREKVGFLEIAKFLSHHFVKD 149

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BLAST of Carg06367-RA vs. NCBI nr
Match: KAG6597212.1 (hypothetical protein SDJN03_10392, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 240.7 bits (613), Expect = 7.6e-60
Identity = 131/142 (92.25%), Postives = 132/142 (92.96%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT 60
           MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT
Sbjct: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT 60

Query: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120
           RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI
Sbjct: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120

Query: 121 FEGREKVGF-LEIAKLLTHHFV 142
           FEGREK     E  KL   HF+
Sbjct: 121 FEGREKAFCKKESTKLEAFHFL 142

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BLAST of Carg06367-RA vs. ExPASy Swiss-Prot
Match: O74503 (Upstream activation factor subunit spp27 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spp27 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.0e-11
Identity = 29/56 (51.79%), Postives = 44/56 (78.57%), Query Frame = 0


Query: 67  KVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAIFE 123
           K +++SP L+ FLG+ ++SR   VK++W YIK ++LQ+P +KR I CDDKLK++FE
Sbjct: 119 KPMKLSPKLAEFLGLEQLSRPQTVKKLWEYIKAHDLQDPNDKRTILCDDKLKSVFE 174

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BLAST of Carg06367-RA vs. ExPASy Swiss-Prot
Match: Q05024 (Protein TRI1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRI1 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.0e-10
Identity = 40/97 (41.24%), Postives = 57/97 (58.76%), Query Frame = 0


Query: 45  GVSAAAPKPKIKRVTTRSTGILKV--VEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNL 104
           G S +  K K K+  +  +  + V  V +S  L  FLG  E+ RT  VK IW YIK ++L
Sbjct: 98  GKSESKSKRKKKKNDSPDSNSISVRKVLLSAPLQKFLGSEELPRTQVVKMIWQYIKEHDL 157

Query: 105 QNPANKREIFCDDKLKAIFEGREKVGFLEIAKLLTHH 140
           QNP ++REI CD+K++ IF   +K+    + KLLT H
Sbjct: 158 QNPKDRREILCDEKMEPIF--GKKMTMFSMNKLLTKH 192

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BLAST of Carg06367-RA vs. ExPASy Swiss-Prot
Match: Q08747 (Upstream activation factor subunit UAF30 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UAF30 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.6e-10
Identity = 37/109 (33.94%), Postives = 61/109 (55.96%), Query Frame = 0


Query: 34  SKEKAPPSSDDGVSAAAPKPKIKRVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQI 93
           SK  +    +D  +      K K   ++S    + V +S +L++ LG  E++RT  V+++
Sbjct: 89  SKRSSGEEKNDSETKGTHVEKKKGTVSKSPISTRKVTLSKSLASLLGEHELTRTEVVRRL 148

Query: 94  WSYIKLNNLQNPANKREIFCDDKLKAIFEGREKVGFLEIAKLLTHHFVK 143
           W+YIK +NLQNP NK+EI CD+KL+ I    +     E+ K+L  H  +
Sbjct: 149 WAYIKAHNLQNPNNKKEILCDEKLELIL--GKSTNMFEMHKILASHMTE 195

[Close]

BLAST of Carg06367-RA vs. ExPASy Swiss-Prot
Match: Q9FMT4 (SWI/SNF complex component SNF12 homolog OS=Arabidopsis thaliana OX=3702 GN=At5g14170 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 8.5e-06
Identity = 24/70 (34.29%), Postives = 40/70 (57.14%), Query Frame = 0


Query: 70  EVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAIFEGREKVGF 129
           ++S AL + LG+   +R   +  IW Y+K   LQNP +     CD  L+ +F G EK+ F
Sbjct: 320 KLSTALMDVLGIEVETRPRIIAAIWHYVKARKLQNPNDPSFFNCDAALQKVF-GEEKLKF 379

Query: 130 LEIAKLLTHH 140
             +++ ++HH
Sbjct: 380 TMVSQKISHH 388

[Close]

BLAST of Carg06367-RA vs. ExPASy Swiss-Prot
Match: Q9P7S3 (SWI/SNF and RSC complexes subunit ssr3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ssr3 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 3.2e-05
Identity = 25/67 (37.31%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 70  EVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAIFEGREKVGF 129
           ++S A +N LG+ E +R + V  +W YIK + LQ+   KR I CD  L+ +FE  +++ F
Sbjct: 207 KLSKAFANILGIREGTRPDIVSYLWQYIKFHRLQDMEEKRLINCDKALRDLFEA-DRLYF 266

Query: 130 LEIAKLL 137
             I +L+
Sbjct: 267 PRIPELM 272

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BLAST of Carg06367-RA vs. ExPASy TrEMBL
Match: A0A6J1GFF6 (upstream activation factor subunit UAF30-like OS=Cucurbita moschata OX=3662 GN=LOC111453685 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.7e-68
Identity = 141/143 (98.60%), Postives = 141/143 (98.60%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTT 60
           MSS FSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDD VSAAAPKPKIKRVTT
Sbjct: 1   MSSGFSNGIRVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDVVSAAAPKPKIKRVTT 60

Query: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120
           RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI
Sbjct: 61  RSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAI 120

Query: 121 FEGREKVGFLEIAKLLTHHFVKD 144
           FEGREKVGFLEIAKLLTHHFVKD
Sbjct: 121 FEGREKVGFLEIAKLLTHHFVKD 143

[Close]

BLAST of Carg06367-RA vs. ExPASy TrEMBL
Match: A0A6J1IKQ4 (protein TRI1-like OS=Cucurbita maxima OX=3661 GN=LOC111474920 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.3e-62
Identity = 134/149 (89.93%), Postives = 136/149 (91.28%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAI------KSKEKAPPSSDDGVSAAAPKPK 60
           MSS FSNGIRVFRGC ALLAPSKSSAAVPA+I      KSKEKAPPSSDD VSAAAPKP 
Sbjct: 1   MSSGFSNGIRVFRGCTALLAPSKSSAAVPASIKSKATSKSKEKAPPSSDDVVSAAAPKPT 60

Query: 61  IKRVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCD 120
           IKR TTR TGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCD
Sbjct: 61  IKRATTRLTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCD 120

Query: 121 DKLKAIFEGREKVGFLEIAKLLTHHFVKD 144
           DKLKAIFEGREKVGFLEIAK L+HHFVKD
Sbjct: 121 DKLKAIFEGREKVGFLEIAKFLSHHFVKD 149

[Close]

BLAST of Carg06367-RA vs. ExPASy TrEMBL
Match: A0A6J1IWB1 (protein TRI1-like OS=Cucurbita maxima OX=3661 GN=LOC111479808 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 9.7e-53
Identity = 120/154 (77.92%), Postives = 129/154 (83.77%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAV-PAAIKS----------KEKAPPSSDDGVSAA 60
           MSS FSNGIRVFRGCRALLAPSKSSA V PAA+KS          KEK PP S D  +AA
Sbjct: 1   MSSGFSNGIRVFRGCRALLAPSKSSATVPPAAVKSRTTSKSNTEPKEKTPPRS-DVAAAA 60

Query: 61  APKPKIKRVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKR 120
           APKPK+KR  TRSTG LKV++VSPAL NFLGVSE SRT+AVKQIWSYIKL+NLQNPANKR
Sbjct: 61  APKPKVKREMTRSTGFLKVLQVSPALGNFLGVSEASRTDAVKQIWSYIKLHNLQNPANKR 120

Query: 121 EIFCDDKLKAIFEGREKVGFLEIAKLLTHHFVKD 144
           EI+CDDKLKAIFEGREKVGFLEI KLLT HFVK+
Sbjct: 121 EIYCDDKLKAIFEGREKVGFLEIGKLLTRHFVKN 153

[Close]

BLAST of Carg06367-RA vs. ExPASy TrEMBL
Match: A0A6J1D0R8 (upstream activation factor subunit spp27-like OS=Momordica charantia OX=3673 GN=LOC111015988 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 2.0e-50
Identity = 116/155 (74.84%), Postives = 124/155 (80.00%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAVPAAI------------KSKEKAPPSSDDGVSA 60
           MSS  SNGIRVFRGCRALLAPSKSSA+VPAA             +SKEK  PSSD   +A
Sbjct: 1   MSSGLSNGIRVFRGCRALLAPSKSSASVPAAAAAKSKATGKPKSESKEKTAPSSDT-AAA 60

Query: 61  AAPKPKIKRVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANK 120
           AAPKPKIKR  TR  GILKV +VSPAL NFLGV E SRT+AVKQIWSYIKL+NLQNPANK
Sbjct: 61  AAPKPKIKREMTRPAGILKVAQVSPALGNFLGVPEASRTDAVKQIWSYIKLHNLQNPANK 120

Query: 121 REIFCDDKLKAIFEGREKVGFLEIAKLLTHHFVKD 144
           REI+CDDKLKAIFEGREKVGFLEI KLL  HFVK+
Sbjct: 121 REIYCDDKLKAIFEGREKVGFLEIGKLLARHFVKN 154

[Close]

BLAST of Carg06367-RA vs. ExPASy TrEMBL
Match: A0A6J1EDA2 (upstream activation factor subunit spp27-like OS=Cucurbita moschata OX=3662 GN=LOC111432134 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 8.5e-49
Identity = 113/154 (73.38%), Postives = 124/154 (80.52%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSKSSAAV-PAAIKS----------KEKAPPSSDDGVSAA 60
           MSS FSNGIRVFRGC +LLAP KSSA V P A+KS          KEK PP S D  +AA
Sbjct: 1   MSSGFSNGIRVFRGCTSLLAPLKSSATVPPTAVKSKTISKSNAEPKEKTPPRS-DVAAAA 60

Query: 61  APKPKIKRVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKR 120
           APKPK +R  TR TG+LKV +VSPAL NFLGVSE SRT+AVKQIWSYIKL+NLQNP+NKR
Sbjct: 61  APKPKFQREMTRPTGLLKVFQVSPALGNFLGVSEASRTDAVKQIWSYIKLHNLQNPSNKR 120

Query: 121 EIFCDDKLKAIFEGREKVGFLEIAKLLTHHFVKD 144
           EI+CDDKLKAIFEGREKVGFLEI KLLT HFVK+
Sbjct: 121 EIYCDDKLKAIFEGREKVGFLEIGKLLTRHFVKN 153

[Close]

BLAST of Carg06367-RA vs. TAIR 10
Match: AT3G03590.1 (SWIB/MDM2 domain superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.1e-32
Identity = 83/149 (55.70%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MSSVFSNGIRVFRGCRALLAPSK--SSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIK-- 60
           MSSV      VFRG R+LLAP+   +S+ V A    K  A P      + A PKPK K  
Sbjct: 1   MSSV---AAMVFRGARSLLAPASRATSSLVSAGSTKKPAAKPK-----AKAKPKPKAKSD 60

Query: 61  ---RVTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFC 120
              + T RSTGI KV  VSP L+ FLG  E SRT+A+K IW+YIK ++LQNPA+KREIFC
Sbjct: 61  SPAKKTPRSTGIFKVTPVSPVLAQFLGTGETSRTDAIKGIWTYIKSHDLQNPADKREIFC 120

Query: 121 DDKLKAIFEGREKVGFLEIAKLLTHHFVK 143
           D+ LK IFEG++KVGFLEI+KLL+ HFVK
Sbjct: 121 DETLKLIFEGKDKVGFLEISKLLSPHFVK 141

[Close]

BLAST of Carg06367-RA vs. TAIR 10
Match: AT2G35605.1 (SWIB/MDM2 domain superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 1.4e-27
Identity = 66/133 (49.62%), Postives = 80/133 (60.15%), Query Frame = 0


Query: 10  RVFRGCRALLAPSKSSAAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTTRSTGILKVV 69
           RVF  CR L+A + SS                               +   + TGILKVV
Sbjct: 3   RVFGACRVLMAKAASSTGA----------------------------KTGRQGTGILKVV 62

Query: 70  EVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAIFEGREKVGF 129
            VS  L+NF+G +EVSRT AVK+IW YIKLNNLQNP NKREI CD++LK IF G++ VGF
Sbjct: 63  PVSKPLANFIGENEVSRTTAVKKIWEYIKLNNLQNPVNKREILCDEQLKTIFSGKDTVGF 107

Query: 130 LEIAKLLTHHFVK 143
           LEI+KLL+ HF K
Sbjct: 123 LEISKLLSQHFPK 107

[Close]

BLAST of Carg06367-RA vs. TAIR 10
Match: AT4G34290.1 (SWIB/MDM2 domain superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 6.7e-22
Identity = 56/127 (44.09%), Postives = 82/127 (64.57%), Query Frame = 0


Query: 20  APSKSSAAVPAAIKSKEKAPPS-----SDDGVSAAAPKPKIKRVTTRSTGILKVVEVSPA 79
           AP +SS   P++++     P +     +    +AA+  P     T    GI+K   VS A
Sbjct: 18  APLRSSMLSPSSLRLSPNHPTNLRMVRAVTSAAAASSDPTTTTKTREPRGIMKPRPVSQA 77

Query: 80  LSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAIFEGREKVGFLEIAK 139
           + + +GV E+ RT A+K+IW+YIK ++LQ+P NKR+I CD+KLK IFEG+E+VGFLEIAK
Sbjct: 78  MQDVVGVPEIPRTQALKRIWAYIKEHDLQDPQNKRDILCDEKLKKIFEGKERVGFLEIAK 137

Query: 140 LLTHHFV 142
           L+  HF+
Sbjct: 138 LIGPHFL 144

[Close]

BLAST of Carg06367-RA vs. TAIR 10
Match: AT1G31760.1 (SWIB/MDM2 domain superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 1.1e-21
Identity = 47/85 (55.29%), Postives = 62/85 (72.94%), Query Frame = 0


Query: 58  VTTRSTGILKVVEVSPALSNFLGVSEVSRTNAVKQIWSYIKLNNLQNPANKREIFCDDKL 117
           V     GI K   VS  L++F G  E++R +A+K++W Y+KL+NLQNPANK+EI CDDKL
Sbjct: 26  VAGEGKGIFKTRPVSQQLASFAGERELTRGSALKKVWEYVKLHNLQNPANKKEIHCDDKL 85

Query: 118 KAIFEGREKVGFLEIAKLLTHHFVK 143
           K IF+G++KVG  EI KLL+ HF K
Sbjct: 86  KTIFDGKDKVGITEIMKLLSPHFPK 110

[Close]

BLAST of Carg06367-RA vs. TAIR 10
Match: AT2G14880.1 (SWIB/MDM2 domain superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 2.8e-20
Identity = 53/116 (45.69%), Postives = 77/116 (66.38%), Query Frame = 0


Query: 26  AAVPAAIKSKEKAPPSSDDGVSAAAPKPKIKRVTTRSTGILKVVEVSPALSNFLGVSEVS 85
           A+ PAA     +A  S+ +     A   ++ R      GI+K   VSP + + + + E++
Sbjct: 32  ASHPAAKLRLVRAVTSATESSEPTATNKRVPR------GIMKPRPVSPEMQDIVELPEIA 91

Query: 86  RTNAVKQIWSYIKLNNLQNPANKREIFCDDKLKAIFEGREKVGFLEIAKLLTHHFV 142
           RT A+K+IW+YIK ++LQ+P NKREI CD+KLK IFEGR++VGFLEIAKL+  HF+
Sbjct: 92  RTQALKRIWAYIKEHDLQDPQNKREILCDEKLKKIFEGRDRVGFLEIAKLIGPHFL 141

The following BLAST results are available for this feature:

BLAST of Carg06367-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028683.1	1.1e-69	100.00	spp27 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022950651.1	7.5e-68	98.60	upstream activation factor subunit UAF30-like [Cucurbita moschata] >XP_022950661...	[more]
XP_023538991.1	1.1e-66	97.20	upstream activation factor subunit UAF30-like [Cucurbita pepo subsp. pepo]	[more]
XP_022974194.1	4.7e-62	89.93	protein TRI1-like [Cucurbita maxima] >XP_022975509.1 protein TRI1-like [Cucurbit...	[more]
KAG6597212.1	7.6e-60	92.25	hypothetical protein SDJN03_10392, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Carg06367-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O74503	4.0e-11	51.79	Upstream activation factor subunit spp27 OS=Schizosaccharomyces pombe (strain 97...	[more]
Q05024	2.0e-10	41.24	Protein TRI1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 ...	[more]
Q08747	2.6e-10	33.94	Upstream activation factor subunit UAF30 OS=Saccharomyces cerevisiae (strain ATC...	[more]
Q9FMT4	8.5e-06	34.29	SWI/SNF complex component SNF12 homolog OS=Arabidopsis thaliana OX=3702 GN=At5g1...	[more]
Q9P7S3	3.2e-05	37.31	SWI/SNF and RSC complexes subunit ssr3 OS=Schizosaccharomyces pombe (strain 972 ...	[more]

BLAST of Carg06367-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GFF6	3.7e-68	98.60	upstream activation factor subunit UAF30-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1IKQ4	2.3e-62	89.93	protein TRI1-like OS=Cucurbita maxima OX=3661 GN=LOC111474920 PE=4 SV=1	[more]
A0A6J1IWB1	9.7e-53	77.92	protein TRI1-like OS=Cucurbita maxima OX=3661 GN=LOC111479808 PE=4 SV=1	[more]
A0A6J1D0R8	2.0e-50	74.84	upstream activation factor subunit spp27-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1EDA2	8.5e-49	73.38	upstream activation factor subunit spp27-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of Carg06367-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03590.1	1.1e-32	55.70	SWIB/MDM2 domain superfamily protein	[more]
AT2G35605.1	1.4e-27	49.62	SWIB/MDM2 domain superfamily protein	[more]
AT4G34290.1	6.7e-22	44.09	SWIB/MDM2 domain superfamily protein	[more]
AT1G31760.1	1.1e-21	55.29	SWIB/MDM2 domain superfamily protein	[more]
AT2G14880.1	2.8e-20	45.69	SWIB/MDM2 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019835	SWIB domain	SMART	SM00151	swib_2	coord: 65..143 e-value: 6.1E-31 score: 118.8
IPR036885	SWIB/MDM2 domain superfamily	GENE3D	1.10.245.10	SWIB/MDM2 domain	coord: 59..143 e-value: 4.5E-28 score: 99.1
IPR036885	SWIB/MDM2 domain superfamily	SUPERFAMILY	47592	SWIB/MDM2 domain	coord: 61..142
IPR003121	SWIB/MDM2 domain	PFAM	PF02201	SWIB	coord: 69..140 e-value: 2.1E-30 score: 104.4
IPR003121	SWIB/MDM2 domain	PROSITE	PS51925	SWIB_MDM2	coord: 64..142 score: 22.732538
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..55
None	No IPR available	PANTHER	PTHR13844	SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D	coord: 1..142
None	No IPR available	PANTHER	PTHR13844:SF67	PROTEIN TRI1	coord: 1..142
None	No IPR available	CDD	cd10567	SWIB-MDM2_like	coord: 69..140 e-value: 1.94449E-35 score: 115.333

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg06367	Carg06367	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg06367-RA:1	Carg06367-RA:1	exon
Carg06367-RA:2	Carg06367-RA:2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg06367-RA:five_prime_utr	Carg06367-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg06367-RA:cds	Carg06367-RA:cds	CDS
Carg06367-RA:cds	Carg06367-RA:cds_2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg06367-RA:three_prime_utr	Carg06367-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg06367-RA	Carg06367-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus