Carg05222-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05222-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ethylene-responsive transcription factor ERF109-like
Location	Carg_Chr07: 3599961 .. 3600599 (+)
Sequence length	639
RNA-Seq Expression	Carg05222-RA
Synteny	Carg05222-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGGTTCTTCCGGAGATTTGGATTCTGCGCCGTTTCACTCGACGTGTAAATTAGCAGTTGAAGAGGAGCATTCGATCATGGTTTCGGCACTGACACACGTGGTCAGCGACGGCAGAATTGGGCTATCCCACACCCAGTGCCAGCTTCTAGCAGCTTTTGACGACGCTCAGCTTCCAGCTATTGAACTCTGGTTTCCCAGTGGCGACACGTGCGAGGTGTGCCGGATTGTTGGCTGCTTGGGATGCAACTATTTCCAAGACGGAAATTATAATCACAATCATAATCAAAATCAAAATCAAATTCAGATTCAAAATGTTGTGCCCGTAGAAGAAGATGAGGAGAAAGATCAAGATGAAGAGAAGAAAATAGTTGTGGGCAATAAGAAGAGAAGGATGAAGAGGACATTCAGGGGAGTGAGGCAGAGGCCTTGGGGGAAATGGGCAGCCGAGATTCGTGACCCGAGACGGGCCACCAGAGTGTGGCTAGGCACTTTCGACACAGCAGAGCAAGCGGCGAGGGCTTATGACAGAGCTGCCATTGAGTTCCGTGGAGATAAGGCCAAGCTCAACTTTCCTGCTTCAGACTACCAAACCACAGAGCAGGAAGAGAAACCCAAATTGGAAATCCAGGAATGA

mRNA sequence

ATGTCTGGTTCTTCCGGAGATTTGGATTCTGCGCCGTTTCACTCGACGTGTAAATTAGCAGTTGAAGAGGAGCATTCGATCATGGTTTCGGCACTGACACACGTGGTCAGCGACGGCAGAATTGGGCTATCCCACACCCAGTGCCAGCTTCTAGCAGCTTTTGACGACGCTCAGCTTCCAGCTATTGAACTCTGGTTTCCCAGTGGCGACACGTGCGAGGTGTGCCGGATTGTTGGCTGCTTGGGATGCAACTATTTCCAAGACGGAAATTATAATCACAATCATAATCAAAATCAAAATCAAATTCAGATTCAAAATGTTGTGCCCGTAGAAGAAGATGAGGAGAAAGATCAAGATGAAGAGAAGAAAATAGTTGTGGGCAATAAGAAGAGAAGGATGAAGAGGACATTCAGGGGAGTGAGGCAGAGGCCTTGGGGGAAATGGGCAGCCGAGATTCGTGACCCGAGACGGGCCACCAGAGTGTGGCTAGGCACTTTCGACACAGCAGAGCAAGCGGCGAGGGCTTATGACAGAGCTGCCATTGAGTTCCGTGGAGATAAGGCCAAGCTCAACTTTCCTGCTTCAGACTACCAAACCACAGAGCAGGAAGAGAAACCCAAATTGGAAATCCAGGAATGA

Coding sequence (CDS)

ATGTCTGGTTCTTCCGGAGATTTGGATTCTGCGCCGTTTCACTCGACGTGTAAATTAGCAGTTGAAGAGGAGCATTCGATCATGGTTTCGGCACTGACACACGTGGTCAGCGACGGCAGAATTGGGCTATCCCACACCCAGTGCCAGCTTCTAGCAGCTTTTGACGACGCTCAGCTTCCAGCTATTGAACTCTGGTTTCCCAGTGGCGACACGTGCGAGGTGTGCCGGATTGTTGGCTGCTTGGGATGCAACTATTTCCAAGACGGAAATTATAATCACAATCATAATCAAAATCAAAATCAAATTCAGATTCAAAATGTTGTGCCCGTAGAAGAAGATGAGGAGAAAGATCAAGATGAAGAGAAGAAAATAGTTGTGGGCAATAAGAAGAGAAGGATGAAGAGGACATTCAGGGGAGTGAGGCAGAGGCCTTGGGGGAAATGGGCAGCCGAGATTCGTGACCCGAGACGGGCCACCAGAGTGTGGCTAGGCACTTTCGACACAGCAGAGCAAGCGGCGAGGGCTTATGACAGAGCTGCCATTGAGTTCCGTGGAGATAAGGCCAAGCTCAACTTTCCTGCTTCAGACTACCAAACCACAGAGCAGGAAGAGAAACCCAAATTGGAAATCCAGGAATGA

Protein sequence

MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLPAIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAAIEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE

Homology

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BLAST of Carg05222-RA vs. NCBI nr
Match: KAG7027062.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 427.2 bits (1097), Expect = 8.4e-116
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP
Sbjct: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60

Query: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120
           AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE
Sbjct: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120

Query: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180
           EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA
Sbjct: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180

Query: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 213
           IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE
Sbjct: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 212

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BLAST of Carg05222-RA vs. NCBI nr
Match: KAG6595039.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 424.5 bits (1090), Expect = 5.5e-115
Identity = 211/212 (99.53%), Postives = 211/212 (99.53%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP
Sbjct: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60

Query: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120
           AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE
Sbjct: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120

Query: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180
           EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTF TAEQAARAYDRAA
Sbjct: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFGTAEQAARAYDRAA 180

Query: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 213
           IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE
Sbjct: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 212

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BLAST of Carg05222-RA vs. NCBI nr
Match: XP_022963153.1 (ethylene-responsive transcription factor ERF109-like [Cucurbita moschata])

HSP 1 Score: 415.2 bits (1066), Expect = 3.3e-112
Identity = 207/212 (97.64%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGL+HTQ QLLAAFDDAQLP
Sbjct: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLTHTQYQLLAAFDDAQLP 60

Query: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120
           AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVP EEDEEKDQDE
Sbjct: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPEEEDEEKDQDE 120

Query: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180
           E+KIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA
Sbjct: 121 EEKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180

Query: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 213
           IEFRGDKAKLNFPASDYQTTEQ EKPKLEIQE
Sbjct: 181 IEFRGDKAKLNFPASDYQTTEQGEKPKLEIQE 212

[Close]

BLAST of Carg05222-RA vs. NCBI nr
Match: XP_023518712.1 (ethylene-responsive transcription factor ERF109-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.9 bits (1047), Expect = 5.3e-110
Identity = 205/212 (96.70%), Postives = 208/212 (98.11%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGL+HTQ QLLAAFDDAQLP
Sbjct: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLTHTQYQLLAAFDDAQLP 60

Query: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120
           AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNH  NQNQIQIQNVVPVEEDEEKDQDE
Sbjct: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNH--NQNQIQIQNVVPVEEDEEKDQDE 120

Query: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180
           EKKIVVGNKKRRMKR FRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAE+AARAYDRAA
Sbjct: 121 EKKIVVGNKKRRMKRRFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEKAARAYDRAA 180

Query: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 213
           IEFRGDKAKLNFPASDYQTTEQE+KPKLEIQE
Sbjct: 181 IEFRGDKAKLNFPASDYQTTEQEQKPKLEIQE 210

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BLAST of Carg05222-RA vs. NCBI nr
Match: XP_023003418.1 (ethylene-responsive transcription factor ERF109-like [Cucurbita maxima])

HSP 1 Score: 404.8 bits (1039), Expect = 4.5e-109
Identity = 203/212 (95.75%), Postives = 205/212 (96.70%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSGSSGDLDS PFHSTCKLAVEEEHSIMVSALTHVVSDGRIGL+HTQ QLLAAFDDAQLP
Sbjct: 1   MSGSSGDLDSVPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLTHTQYQLLAAFDDAQLP 60

Query: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120
           AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHN N    QIQNVVPVEEDEEKDQDE
Sbjct: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNHN----QIQNVVPVEEDEEKDQDE 120

Query: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180
           EKKI+VGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA
Sbjct: 121 EKKIIVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180

Query: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 213
           IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE
Sbjct: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 208

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BLAST of Carg05222-RA vs. ExPASy Swiss-Prot
Match: Q9SZ06 (Ethylene-responsive transcription factor ERF109 OS=Arabidopsis thaliana OX=3702 GN=ERF109 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 7.6e-35
Identity = 91/187 (48.66%), Postives = 118/187 (63.10%), Query Frame = 0


Query: 18  KLAVEEEHSIMVSALTHVVSDGRIGLSHT-QCQLLAAFDDAQLPAIELWFPSGDTCEVCR 77
           +L+ E+E S++VSAL HV+S    G + T  CQ  ++  D+ + +  +     DTC+VCR
Sbjct: 23  QLSPEQELSVIVSALQHVIS----GENETAPCQGFSS--DSTVISAGMPRLDSDTCQVCR 82

Query: 78  IVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDEEKKIVVGNKKRRMKRT 137
           I GCLGCNYF    +  N    +N  Q + +        +     KK   G K R+ K  
Sbjct: 83  IEGCLGCNYF----FAPNQRIEKNHQQEEEITSSSNRRRESSPVAKKAEGGGKIRKRKNK 142

Query: 138 ---FRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAAIEFRGDKAKLNFP 197
              +RGVRQRPWGK+AAEIRDP+RATRVWLGTF+TAE AARAYDRAAI FRG +AKLNFP
Sbjct: 143 KNGYRGVRQRPWGKFAAEIRDPKRATRVWLGTFETAEDAARAYDRAAIGFRGPRAKLNFP 199

Query: 198 ASDYQTT 201
             DY ++
Sbjct: 203 FVDYTSS 199

[Close]

BLAST of Carg05222-RA vs. ExPASy Swiss-Prot
Match: Q70II3 (Ethylene-responsive transcription factor ERF110 OS=Arabidopsis thaliana OX=3702 GN=ERF110 PE=2 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 3.9e-23
Identity = 58/85 (68.24%), Postives = 62/85 (72.94%), Query Frame = 0


Query: 109 PVEEDEEKDQDEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDT 168
           P+EE   K   EE         R  KR +RGVRQRPWGKWAAEIRDP RA RVWLGTFDT
Sbjct: 57  PIEESMSKAFPEE--------AREKKRRYRGVRQRPWGKWAAEIRDPHRAARVWLGTFDT 116

Query: 169 AEQAARAYDRAAIEFRGDKAKLNFP 194
           AE AARAYD AA+ FRG+KAKLNFP
Sbjct: 117 AEAAARAYDEAALRFRGNKAKLNFP 133

[Close]

BLAST of Carg05222-RA vs. ExPASy Swiss-Prot
Match: P93007 (Ethylene-responsive transcription factor ERF112 OS=Arabidopsis thaliana OX=3702 GN=ERF112 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.9e-23
Identity = 51/75 (68.00%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 119 DEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDR 178
           D+   + +  K    +R +RGVRQRPWGKWAAEIRDP +A RVWLGTFDTAE+AA AYD+
Sbjct: 52  DQSSSLTLQEKSNSRQRNYRGVRQRPWGKWAAEIRDPNKAARVWLGTFDTAEEAALAYDK 111

Query: 179 AAIEFRGDKAKLNFP 194
           AA EFRG KAKLNFP
Sbjct: 112 AAFEFRGHKAKLNFP 126

[Close]

BLAST of Carg05222-RA vs. ExPASy Swiss-Prot
Match: Q7G1L2 (Ethylene-responsive transcription factor RAP2-6 OS=Arabidopsis thaliana OX=3702 GN=RAP2-6 PE=2 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 4.3e-22
Identity = 48/59 (81.36%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 135 RTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAAIEFRGDKAKLNFP 194
           + +RGVRQRPWGKWAAEIRDP +ATRVWLGTF+TAE AARAYD AA+ FRG KAKLNFP
Sbjct: 59  KKYRGVRQRPWGKWAAEIRDPHKATRVWLGTFETAEAAARAYDAAALRFRGSKAKLNFP 117

[Close]

BLAST of Carg05222-RA vs. ExPASy Swiss-Prot
Match: Q9FGF8 (Ethylene-responsive transcription factor ABR1 OS=Arabidopsis thaliana OX=3702 GN=ABR1 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 7.4e-22
Identity = 48/60 (80.00%), Postives = 53/60 (88.33%), Query Frame = 0


Query: 134 KRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAAIEFRGDKAKLNFP 193
           +R +RGVRQRPWGKWAAEIRDP +A RVWLGTFD AE AARAYD AA+ FRG+KAKLNFP
Sbjct: 182 RRRYRGVRQRPWGKWAAEIRDPFKAARVWLGTFDNAESAARAYDEAALRFRGNKAKLNFP 241

[Close]

BLAST of Carg05222-RA vs. ExPASy TrEMBL
Match: A0A6J1HGX3 (ethylene-responsive transcription factor ERF109-like OS=Cucurbita moschata OX=3662 GN=LOC111463448 PE=4 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.6e-112
Identity = 207/212 (97.64%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGL+HTQ QLLAAFDDAQLP
Sbjct: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLTHTQYQLLAAFDDAQLP 60

Query: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120
           AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVP EEDEEKDQDE
Sbjct: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPEEEDEEKDQDE 120

Query: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180
           E+KIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA
Sbjct: 121 EEKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180

Query: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 213
           IEFRGDKAKLNFPASDYQTTEQ EKPKLEIQE
Sbjct: 181 IEFRGDKAKLNFPASDYQTTEQGEKPKLEIQE 212

[Close]

BLAST of Carg05222-RA vs. ExPASy TrEMBL
Match: A0A6J1KWF8 (ethylene-responsive transcription factor ERF109-like OS=Cucurbita maxima OX=3661 GN=LOC111497034 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.2e-109
Identity = 203/212 (95.75%), Postives = 205/212 (96.70%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSGSSGDLDS PFHSTCKLAVEEEHSIMVSALTHVVSDGRIGL+HTQ QLLAAFDDAQLP
Sbjct: 1   MSGSSGDLDSVPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLTHTQYQLLAAFDDAQLP 60

Query: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDE 120
           AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHN N    QIQNVVPVEEDEEKDQDE
Sbjct: 61  AIELWFPSGDTCEVCRIVGCLGCNYFQDGNYNHNHNHN----QIQNVVPVEEDEEKDQDE 120

Query: 121 EKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180
           EKKI+VGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA
Sbjct: 121 EKKIIVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAA 180

Query: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 213
           IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE
Sbjct: 181 IEFRGDKAKLNFPASDYQTTEQEEKPKLEIQE 208

[Close]

BLAST of Carg05222-RA vs. ExPASy TrEMBL
Match: A0A1S3B0B5 (ethylene-responsive transcription factor ERF109-like OS=Cucumis melo OX=3656 GN=LOC103484773 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.6e-48
Identity = 124/221 (56.11%), Postives = 148/221 (66.97%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAF------ 60
           MSG SG+  +APF S  +L VEEE+SIMVSALTHV++D RIGL+H Q QLL +       
Sbjct: 1   MSGFSGNFVTAPFGS--RLGVEEENSIMVSALTHVLTDSRIGLTHAQYQLLFSSNLDHND 60

Query: 61  -DDAQLPAIELWFPSG-DTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEE 120
            D        LWFPS  D C+ C+  GCLGCNYF+DG+     NQNQN         V +
Sbjct: 61  DDSNNNSGNVLWFPSAMDECKKCQFAGCLGCNYFEDGS-----NQNQN---------VVK 120

Query: 121 DEEKDQDEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQA 180
           DEE D   +KK     +++++K  FRGVRQR WGKWAAEIRDPRR  RVWLGTF TAE+A
Sbjct: 121 DEENDNKNKKK-----RRKKIKGRFRGVRQRRWGKWAAEIRDPRRKVRVWLGTFQTAEEA 180

Query: 181 ARAYDRAAIEFRG-DKAKLNFPASDYQTTEQEE---KPKLE 210
           ARAYDRAAIEFRG  +AKLNFPASDYQ  +  +   +P LE
Sbjct: 181 ARAYDRAAIEFRGIGRAKLNFPASDYQQNQIPQGYREPNLE 200

[Close]

BLAST of Carg05222-RA vs. ExPASy TrEMBL
Match: A0A5D3CM46 (Ethylene-responsive transcription factor ERF109-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004850 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 2.8e-48
Identity = 120/207 (57.97%), Postives = 143/207 (69.08%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAF------ 60
           MSG SG+L +APF S  +L VEEE+SIMVSALTHV++D RIGL+H Q QLL +       
Sbjct: 1   MSGFSGNLVTAPFGS--RLGVEEENSIMVSALTHVLTDSRIGLTHAQYQLLFSSNLDHND 60

Query: 61  -DDAQLPAIELWFPSG-DTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEE 120
            +        LWFPS  D C+ C+  GCLGCNYF+DG+     NQNQN         V +
Sbjct: 61  DNSNNNSGNVLWFPSAIDECKKCQFAGCLGCNYFEDGS-----NQNQN---------VVK 120

Query: 121 DEEKDQDEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQA 180
           DEE D   +KK     +++++K  FRGVRQR WGKWAAEIRDPRR  RVWLGTF TAE+A
Sbjct: 121 DEENDNKNKKK-----RRKKIKGRFRGVRQRRWGKWAAEIRDPRRKVRVWLGTFQTAEEA 180

Query: 181 ARAYDRAAIEFRG-DKAKLNFPASDYQ 199
           ARAYDRAAI+FRG  +AKLNFPASDYQ
Sbjct: 181 ARAYDRAAIKFRGIGRAKLNFPASDYQ 186

[Close]

BLAST of Carg05222-RA vs. ExPASy TrEMBL
Match: A0A0A0KJJ4 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490860 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.2e-43
Identity = 117/214 (54.67%), Postives = 140/214 (65.42%), Query Frame = 0


Query: 1   MSGSSGDLDSAPFHSTCKLAVEEEHSIMVSALTHVVSDGRIGLSHTQCQLLAAFDDAQLP 60
           MSG S DL +APF S  +L VEEE+SI+VSALT+V++D RIGL H        + +    
Sbjct: 1   MSGPSRDLVTAPFGS--RLGVEEENSIIVSALTYVLTDTRIGLDHND-----DYSNNHSG 60

Query: 61  AIELWFPS-GDTCEVCRIVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQD 120
              LWFPS  D C+ C+   CLGCNYF+DGN     NQNQN         V +DE+ + +
Sbjct: 61  TAVLWFPSANDECKKCQFANCLGCNYFEDGN-----NQNQN---------VVKDEDNNDN 120

Query: 121 EEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRA 180
             KK      +++MK  FRGVR+R  GKWAAEIRDPRR  RVWLGTF TAE+AARAYDRA
Sbjct: 121 GNKK----KGRKKMKGRFRGVRERRRGKWAAEIRDPRRRIRVWLGTFQTAEEAARAYDRA 180

Query: 181 AIEFR-GDKAKLNFPASDYQTTE---QEEKPKLE 210
           AIEFR GD+AKLNFPASDYQ  E      +P LE
Sbjct: 181 AIEFRGGDRAKLNFPASDYQQNEIPQGNREPNLE 189

[Close]

BLAST of Carg05222-RA vs. TAIR 10
Match: AT4G34410.1 (redox responsive transcription factor 1 )

HSP 1 Score: 148.7 bits (374), Expect = 5.4e-36
Identity = 91/187 (48.66%), Postives = 118/187 (63.10%), Query Frame = 0


Query: 18  KLAVEEEHSIMVSALTHVVSDGRIGLSHT-QCQLLAAFDDAQLPAIELWFPSGDTCEVCR 77
           +L+ E+E S++VSAL HV+S    G + T  CQ  ++  D+ + +  +     DTC+VCR
Sbjct: 23  QLSPEQELSVIVSALQHVIS----GENETAPCQGFSS--DSTVISAGMPRLDSDTCQVCR 82

Query: 78  IVGCLGCNYFQDGNYNHNHNQNQNQIQIQNVVPVEEDEEKDQDEEKKIVVGNKKRRMKRT 137
           I GCLGCNYF    +  N    +N  Q + +        +     KK   G K R+ K  
Sbjct: 83  IEGCLGCNYF----FAPNQRIEKNHQQEEEITSSSNRRRESSPVAKKAEGGGKIRKRKNK 142

Query: 138 ---FRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAAIEFRGDKAKLNFP 197
              +RGVRQRPWGK+AAEIRDP+RATRVWLGTF+TAE AARAYDRAAI FRG +AKLNFP
Sbjct: 143 KNGYRGVRQRPWGKFAAEIRDPKRATRVWLGTFETAEDAARAYDRAAIGFRGPRAKLNFP 199

Query: 198 ASDYQTT 201
             DY ++
Sbjct: 203 FVDYTSS 199

[Close]

BLAST of Carg05222-RA vs. TAIR 10
Match: AT2G33710.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-24
Identity = 51/75 (68.00%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 119 DEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDR 178
           D+   + +  K    +R +RGVRQRPWGKWAAEIRDP +A RVWLGTFDTAE+AA AYD+
Sbjct: 52  DQSSSLTLQEKSNSRQRNYRGVRQRPWGKWAAEIRDPNKAARVWLGTFDTAEEAALAYDK 111

Query: 179 AAIEFRGDKAKLNFP 194
           AA EFRG KAKLNFP
Sbjct: 112 AAFEFRGHKAKLNFP 126

[Close]

BLAST of Carg05222-RA vs. TAIR 10
Match: AT2G33710.2 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-24
Identity = 51/75 (68.00%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 119 DEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDR 178
           D+   + +  K    +R +RGVRQRPWGKWAAEIRDP +A RVWLGTFDTAE+AA AYD+
Sbjct: 52  DQSSSLTLQEKSNSRQRNYRGVRQRPWGKWAAEIRDPNKAARVWLGTFDTAEEAALAYDK 111

Query: 179 AAIEFRGDKAKLNFP 194
           AA EFRG KAKLNFP
Sbjct: 112 AAFEFRGHKAKLNFP 126

[Close]

BLAST of Carg05222-RA vs. TAIR 10
Match: AT5G50080.1 (ethylene response factor 110 )

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-24
Identity = 58/85 (68.24%), Postives = 62/85 (72.94%), Query Frame = 0


Query: 109 PVEEDEEKDQDEEKKIVVGNKKRRMKRTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDT 168
           P+EE   K   EE         R  KR +RGVRQRPWGKWAAEIRDP RA RVWLGTFDT
Sbjct: 57  PIEESMSKAFPEE--------AREKKRRYRGVRQRPWGKWAAEIRDPHRAARVWLGTFDT 116

Query: 169 AEQAARAYDRAAIEFRGDKAKLNFP 194
           AE AARAYD AA+ FRG+KAKLNFP
Sbjct: 117 AEAAARAYDEAALRFRGNKAKLNFP 133

[Close]

BLAST of Carg05222-RA vs. TAIR 10
Match: AT1G43160.1 (related to AP2 6 )

HSP 1 Score: 106.3 bits (264), Expect = 3.1e-23
Identity = 48/59 (81.36%), Postives = 53/59 (89.83%), Query Frame = 0


Query: 135 RTFRGVRQRPWGKWAAEIRDPRRATRVWLGTFDTAEQAARAYDRAAIEFRGDKAKLNFP 194
           + +RGVRQRPWGKWAAEIRDP +ATRVWLGTF+TAE AARAYD AA+ FRG KAKLNFP
Sbjct: 59  KKYRGVRQRPWGKWAAEIRDPHKATRVWLGTFETAEAAARAYDAAALRFRGSKAKLNFP 117

The following BLAST results are available for this feature:

BLAST of Carg05222-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027062.1	8.4e-116	100.00	Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp....	[more]
KAG6595039.1	5.5e-115	99.53	Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp....	[more]
XP_022963153.1	3.3e-112	97.64	ethylene-responsive transcription factor ERF109-like [Cucurbita moschata]	[more]
XP_023518712.1	5.3e-110	96.70	ethylene-responsive transcription factor ERF109-like [Cucurbita pepo subsp. pepo...	[more]
XP_023003418.1	4.5e-109	95.75	ethylene-responsive transcription factor ERF109-like [Cucurbita maxima]	[more]

BLAST of Carg05222-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ06	7.6e-35	48.66	Ethylene-responsive transcription factor ERF109 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q70II3	3.9e-23	68.24	Ethylene-responsive transcription factor ERF110 OS=Arabidopsis thaliana OX=3702 ...	[more]
P93007	3.9e-23	68.00	Ethylene-responsive transcription factor ERF112 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q7G1L2	4.3e-22	81.36	Ethylene-responsive transcription factor RAP2-6 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9FGF8	7.4e-22	80.00	Ethylene-responsive transcription factor ABR1 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Carg05222-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HGX3	1.6e-112	97.64	ethylene-responsive transcription factor ERF109-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1KWF8	2.2e-109	95.75	ethylene-responsive transcription factor ERF109-like OS=Cucurbita maxima OX=3661...	[more]
A0A1S3B0B5	1.6e-48	56.11	ethylene-responsive transcription factor ERF109-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A5D3CM46	2.8e-48	57.97	Ethylene-responsive transcription factor ERF109-like protein OS=Cucumis melo var...	[more]
A0A0A0KJJ4	1.2e-43	54.67	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490860 PE=...	[more]

BLAST of Carg05222-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34410.1	5.4e-36	48.66	redox responsive transcription factor 1	[more]
AT2G33710.1	2.8e-24	68.00	Integrase-type DNA-binding superfamily protein	[more]
AT2G33710.2	2.8e-24	68.00	Integrase-type DNA-binding superfamily protein	[more]
AT5G50080.1	2.8e-24	68.24	ethylene response factor 110	[more]
AT1G43160.1	3.1e-23	81.36	related to AP2 6	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 137..148 score: 70.91 coord: 159..175 score: 73.26
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 136..199 e-value: 3.9E-38 score: 142.7
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 137..185 e-value: 1.4E-15 score: 57.3
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 136..193 score: 24.829386
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 137..195 e-value: 5.33425E-34 score: 113.906
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 136..194 e-value: 5.5E-29 score: 102.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 193..212
None	No IPR available	PANTHER	PTHR31677:SF107	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF109	coord: 15..205
None	No IPR available	PANTHER	PTHR31677	AP2 DOMAIN CLASS TRANSCRIPTION FACTOR	coord: 15..205
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 136..195

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg05222	Carg05222	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg05222-RA:1	Carg05222-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg05222-RA:cds	Carg05222-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg05222-RA	Carg05222-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity