Carg04634-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04634-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-H2 finger protein ATL40-like
Location	Carg_Chr11: 12800853 .. 12801518 (+)
Sequence length	666
RNA-Seq Expression	Carg04634-RA
Synteny	Carg04634-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGCCTCCTCCTTCGACGCCGGCGACTCCCCCTTTTGGGTGGATCACCGCCCTAACCGGAACCCATACAATTTGAACAACAGAATTATGCTCACCGCCATCATTTCCCTTTCTCTTGTCGTTCTTCTCGTCATTGCCCTTCACATTTACGCCCGTTACGCCCTCCGTCGTCACGCCCGACGCCAAGCCGTTCTCCGCCGCCTTGGAATTCTCGCCCTCGTTGACTCCAGCGACCTTCCGCGACCGCCGCGGCACTCCGGTCTCGACCCCCTTGTCATGGCGTCTCTGCCAGTCGTCGTTTTCAAACACCAATTGGAAAACGCAGCTGCCGTTGAGTGCGCCGTGTGTTTGTGCGCCGTCGAGGACGGCGAGGAAGCGAGAGTTTTACCCAATTGTAAACATACTTTCCATTTGGAATGTATTGACAAATGGTTTGGGTTGCATTCCACGTGTCCAAATTGCCGGACGGAGGCGGCGCCGATGATTCGGCCGGAACCCCGGGAGGCTCCGGTGGCGGAGAGCGGTGGTGGGTCAAGCTCCCGATTGAGTTCATTTAGACGGATTCTAAGTAGAGAAAGGTCTTCAAGAAGAATTCAACCTTGTGTGCGCGAAAATGGAAACGACGACGTTCAAGACTTGGAGAGGCAGCAAATGTCAATGTAA

mRNA sequence

ATGTCCGCCTCCTCCTTCGACGCCGGCGACTCCCCCTTTTGGGTGGATCACCGCCCTAACCGGAACCCATACAATTTGAACAACAGAATTATGCTCACCGCCATCATTTCCCTTTCTCTTGTCGTTCTTCTCGTCATTGCCCTTCACATTTACGCCCGTTACGCCCTCCGTCGTCACGCCCGACGCCAAGCCGTTCTCCGCCGCCTTGGAATTCTCGCCCTCGTTGACTCCAGCGACCTTCCGCGACCGCCGCGGCACTCCGGTCTCGACCCCCTTGTCATGGCGTCTCTGCCAGTCGTCGTTTTCAAACACCAATTGGAAAACGCAGCTGCCGTTGAGTGCGCCGTGTGTTTGTGCGCCGTCGAGGACGGCGAGGAAGCGAGAGTTTTACCCAATTGTAAACATACTTTCCATTTGGAATGTATTGACAAATGGTTTGGGTTGCATTCCACGTGTCCAAATTGCCGGACGGAGGCGGCGCCGATGATTCGGCCGGAACCCCGGGAGGCTCCGGTGGCGGAGAGCGGTGGTGGGTCAAGCTCCCGATTGAGTTCATTTAGACGGATTCTAAGTAGAGAAAGGTCTTCAAGAAGAATTCAACCTTGTGTGCGCGAAAATGGAAACGACGACGTTCAAGACTTGGAGAGGCAGCAAATGTCAATGTAA

Coding sequence (CDS)

ATGTCCGCCTCCTCCTTCGACGCCGGCGACTCCCCCTTTTGGGTGGATCACCGCCCTAACCGGAACCCATACAATTTGAACAACAGAATTATGCTCACCGCCATCATTTCCCTTTCTCTTGTCGTTCTTCTCGTCATTGCCCTTCACATTTACGCCCGTTACGCCCTCCGTCGTCACGCCCGACGCCAAGCCGTTCTCCGCCGCCTTGGAATTCTCGCCCTCGTTGACTCCAGCGACCTTCCGCGACCGCCGCGGCACTCCGGTCTCGACCCCCTTGTCATGGCGTCTCTGCCAGTCGTCGTTTTCAAACACCAATTGGAAAACGCAGCTGCCGTTGAGTGCGCCGTGTGTTTGTGCGCCGTCGAGGACGGCGAGGAAGCGAGAGTTTTACCCAATTGTAAACATACTTTCCATTTGGAATGTATTGACAAATGGTTTGGGTTGCATTCCACGTGTCCAAATTGCCGGACGGAGGCGGCGCCGATGATTCGGCCGGAACCCCGGGAGGCTCCGGTGGCGGAGAGCGGTGGTGGGTCAAGCTCCCGATTGAGTTCATTTAGACGGATTCTAAGTAGAGAAAGGTCTTCAAGAAGAATTCAACCTTGTGTGCGCGAAAATGGAAACGACGACGTTCAAGACTTGGAGAGGCAGCAAATGTCAATGTAA

Protein sequence

MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCAVEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM

Homology

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BLAST of Carg04634-RA vs. NCBI nr
Match: KAG6589265.1 (E3 ubiquitin-protein ligase ATL41, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022964.1 E3 ubiquitin-protein ligase ATL41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 439.5 bits (1129), Expect = 1.7e-119
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 60
           MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA
Sbjct: 1   MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 60

Query: 61  RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCA 120
           RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCA
Sbjct: 61  RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCA 120

Query: 121 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS 180
           VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS
Sbjct: 121 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS 180

Query: 181 SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222
           SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM
Sbjct: 181 SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 221

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BLAST of Carg04634-RA vs. NCBI nr
Match: XP_022930874.1 (RING-H2 finger protein ATL40-like [Cucurbita moschata])

HSP 1 Score: 437.6 bits (1124), Expect = 6.5e-119
Identity = 220/221 (99.55%), Postives = 220/221 (99.55%), Query Frame = 0


Query: 1   MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 60
           MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA
Sbjct: 1   MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 60

Query: 61  RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCA 120
           RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLEN AAVECAVCLCA
Sbjct: 61  RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENPAAVECAVCLCA 120

Query: 121 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS 180
           VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS
Sbjct: 121 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS 180

Query: 181 SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222
           SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM
Sbjct: 181 SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 221

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BLAST of Carg04634-RA vs. NCBI nr
Match: XP_023529863.1 (RING-H2 finger protein ATL40-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 423.7 bits (1088), Expect = 9.7e-115
Identity = 217/227 (95.59%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 1   MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 60
           MSASSFD GDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA
Sbjct: 65  MSASSFDGGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 124

Query: 61  RRQAVLRR------LGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVEC 120
           RRQAVLRR      LGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLEN AAVEC
Sbjct: 125 RRQAVLRRQAALRHLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENPAAVEC 184

Query: 121 AVCLCAVEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAE 180
           AVCLCAVEDGEEARVLPNCKHTFHLECIDKWFGLHSTCP CRTEAAPMIRPEPREAPVAE
Sbjct: 185 AVCLCAVEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPICRTEAAPMIRPEPREAPVAE 244

Query: 181 SGGGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222
           SGGGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQ SM
Sbjct: 245 SGGGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQTSM 291

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BLAST of Carg04634-RA vs. NCBI nr
Match: XP_022989093.1 (E3 ubiquitin-protein ligase ATL41-like [Cucurbita maxima])

HSP 1 Score: 411.8 bits (1057), Expect = 3.8e-111
Identity = 212/222 (95.50%), Postives = 214/222 (96.40%), Query Frame = 0


Query: 1   MSASSFDAGDSPFWVDHRPNRN-PYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRH 60
           MSASSFDAGDSPFWVDHR NRN PYNLNNRIML+AIISLSLVVLLVIALHIYARYALRRH
Sbjct: 1   MSASSFDAGDSPFWVDHRSNRNHPYNLNNRIMLSAIISLSLVVLLVIALHIYARYALRRH 60

Query: 61  ARRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLC 120
           ARRQAVLRR GILALV SSDLP PPRHSGLDPLVMASLPVVVFKHQLEN AAVECAVCLC
Sbjct: 61  ARRQAVLRRRGILALVVSSDLPPPPRHSGLDPLVMASLPVVVFKHQLENPAAVECAVCLC 120

Query: 121 AVEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGS 180
           AVE+GEEARVLPNCKHTFHLECIDKWFGLHSTCP CRTEAAPMIRPEPREAPVAESGGGS
Sbjct: 121 AVENGEEARVLPNCKHTFHLECIDKWFGLHSTCPICRTEAAPMIRPEPREAPVAESGGGS 180

Query: 181 SSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222
           SSRLSSF RILSRERSSRRIQPCVRENGNDDVQDLERQQMSM
Sbjct: 181 SSRLSSFGRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222

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BLAST of Carg04634-RA vs. NCBI nr
Match: XP_038877602.1 (RING-H2 finger protein ATL40-like [Benincasa hispida])

HSP 1 Score: 332.8 bits (852), Expect = 2.3e-87
Identity = 178/221 (80.54%), Postives = 188/221 (85.07%), Query Frame = 0


Query: 5   SFDAGDS-PFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQ 64
           SFD GD   F+    P+RNPY+LNN+IMLTAIISLSLVVLLVIALHIYARYALRR ARRQ
Sbjct: 8   SFDDGDGYIFFGPAHPHRNPYDLNNKIMLTAIISLSLVVLLVIALHIYARYALRRQARRQ 67

Query: 65  AVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLEN----AAAVECAVCLC 124
           AVLRRLGILALVDS D P PP  SGLDPLVMAS+PV+VFKHQ EN     AAVECAVCL 
Sbjct: 68  AVLRRLGILALVDSGDPPLPPSRSGLDPLVMASMPVIVFKHQSENPDPSGAAVECAVCLS 127

Query: 125 AVEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAP---VAESG 184
            +EDGE AR+LPNCKH FHLECIDKWFG HSTCP CRTEAAPMI PEPREAP   VAE G
Sbjct: 128 GIEDGETARILPNCKHVFHLECIDKWFGSHSTCPICRTEAAPMILPEPREAPAAAVAEGG 187

Query: 185 GGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQ 218
           GGSSSRLSSFRRILSRERSSRRIQPCVR++ NDDVQDLE Q
Sbjct: 188 GGSSSRLSSFRRILSRERSSRRIQPCVRQSRNDDVQDLESQ 228

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BLAST of Carg04634-RA vs. ExPASy Swiss-Prot
Match: Q9SLC3 (E3 ubiquitin-protein ligase ATL41 OS=Arabidopsis thaliana OX=3702 GN=ATL41 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.0e-34
Identity = 103/239 (43.10%), Postives = 134/239 (56.07%), Query Frame = 0


Query: 6   FDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAV 65
           F++    FW    PN + Y+LN++IML A+ SLS V+L+V ALH+YAR+ LRR  RR+A 
Sbjct: 11  FNSDHHSFW----PNPSTYDLNSKIMLAAVASLSGVILIVFALHLYARFVLRR--RREAF 70

Query: 66  LRRLGILALVDSSDLPRPPRHSGLDPLVMASLPV-VVFKHQLENAAAVECAVCLCAVEDG 125
                 L ++       P R  GL+P V+ASLP   V       A+A ECAVCL  +++ 
Sbjct: 71  RG----LPVIFRHPFEMPKR--GLNPTVIASLPTFTVGATDGVAASATECAVCLSVLKEQ 130

Query: 126 EEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEA--APMIRPEPREAPV---------- 185
           ++AR LPNCKH FH++C+D W    STCP CRTE    P + PEPRE PV          
Sbjct: 131 DKARELPNCKHIFHVDCVDTWLTTCSTCPVCRTEVEPRPRLEPEPREGPVGTAPQLLVET 190

Query: 186 -----AESGGGSS----------SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLER 217
                 E+   SS          SRL+SFR+IL+RERSS RI     +   D V DLER
Sbjct: 191 RLNLTVEAASSSSSDNKTVVSPASRLNSFRKILTRERSSNRINHSCPD--QDRVADLER 235

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BLAST of Carg04634-RA vs. ExPASy Swiss-Prot
Match: Q9SLC4 (RING-H2 finger protein ATL40 OS=Arabidopsis thaliana OX=3702 GN=ATL40 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 4.3e-33
Identity = 91/230 (39.57%), Postives = 127/230 (55.22%), Query Frame = 0


Query: 7   DAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAVL 66
           D+ D  FW     N   Y+ +++I L   +S S+++++V   ++YA++ L R +  Q   
Sbjct: 9   DSDDRSFW----QNSTSYDASSKIFLVTTVSFSIIIIIVFVYYLYAKFVLHRRSAFQD-- 68

Query: 67  RRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCAVEDGEE 126
                   +  S + +PP+  GLD LV+ASLP  V   +  + A  ECAVCL  +E+ + 
Sbjct: 69  --------LSFSVVSQPPKR-GLDSLVIASLPTFVVGIK-NDVAGTECAVCLSLLEEKDN 128

Query: 127 ARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAA---PMIRPEPREAPVAE--------- 186
           AR+LPNCKH FH+ C+D W    STCP CRTEA    P + PEPRE PV +         
Sbjct: 129 ARMLPNCKHVFHVSCVDTWLTTQSTCPVCRTEAEPSHPRLEPEPREGPVGDFAPPLDFAG 188

Query: 187 ----SGGGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQ---DLERQ 218
               +GG S SRL SFRRIL+RERSS R     R++   D     D+ERQ
Sbjct: 189 VDNKTGGSSVSRLDSFRRILTRERSSNR-----RDHSRVDQDRELDIERQ 217

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BLAST of Carg04634-RA vs. ExPASy Swiss-Prot
Match: Q8L9T5 (RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 7.4e-25
Identity = 64/140 (45.71%), Postives = 88/140 (62.86%), Query Frame = 0


Query: 24  YNLNNRIMLTAIISLSLVVLLVIALHIYARY----ALRRHARRQAVLRRLGILALV--DS 83
           Y ++ +IML+AI+ L  VV+L++ LH+YAR+    A RRH RR++  RR  ++      S
Sbjct: 24  YAMSGKIMLSAIVILFFVVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVFFTADPS 83

Query: 84  SDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCAVEDGEEARVLPNCKHTF 143
           +         GLDP V+ SLPV  F  +  +   +ECAVCL   E+ E  RVLPNC+HTF
Sbjct: 84  TAATSVVASRGLDPNVIKSLPVFTFSDE-THKDPIECAVCLSEFEESETGRVLPNCQHTF 143

Query: 144 HLECIDKWFGLHSTCPNCRT 158
           H++CID WF  HSTCP CR+
Sbjct: 144 HVDCIDMWFHSHSTCPLCRS 162

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BLAST of Carg04634-RA vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 71/190 (37.37%), Postives = 102/190 (53.68%), Query Frame = 0


Query: 6   FDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAV 65
           FD+  +  W +     + Y+LN +IML ++I L + V+L++  H YAR+  RR  RR  +
Sbjct: 5   FDSSKT-LWGNMNHGSSRYSLNGKIMLASVIILFVAVILILCFHSYARWLFRRQNRR--I 64

Query: 66  LRRLGILALVDSSDLPRPPRHSG-----LDPLVMASLPVVVFKHQLENAAAVECAVCLCA 125
            RR  I A + S    R P  S      LDP V+  +P+ V+  +   +   EC+VCL  
Sbjct: 65  RRR--ISAHLRSLAAARDPTQSSSSLSPLDPTVLEKIPIFVYSVKTHESPLEECSVCLSE 124

Query: 126 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRT---EAAPMIRPEPREA------P 182
            E+ +E RVLP C H FH++CID WF   S+CP CR     A P+  PEP  A      P
Sbjct: 125 FEEDDEGRVLPKCGHVFHVDCIDTWFRSRSSCPLCRAPVQPAQPVTEPEPVAAVFPSVKP 184

[Close]

BLAST of Carg04634-RA vs. ExPASy Swiss-Prot
Match: O22255 (RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.6e-22
Identity = 64/177 (36.16%), Postives = 92/177 (51.98%), Query Frame = 0


Query: 12  PFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAVLRRLGI 71
           P W       + Y LN +IML+++I L + V++++  H YAR+  RRH RR  + RR+  
Sbjct: 10  PIWGSVSHTSSGYALNGKIMLSSVIVLFVAVIMILCFHSYARWLFRRHNRR--IRRRI-- 69

Query: 72  LALVDSSDLPRPPRHSGLDPLVMASLPVVVF--KHQLENAAAVECAVCLCAVEDGEEARV 131
                   L   PR   LD  V+  +P+ V+  K+        EC+VCL   E+ +E R+
Sbjct: 70  --RSHLRTLSASPRDQALDQAVLDKIPIFVYSSKNPPPPEEKEECSVCLSEFEEEDEGRL 129

Query: 132 LPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSSSRLSSF 187
           LP C H+FH++CID WF   STCP CR    P  +       V E+G  SSS   +F
Sbjct: 130 LPKCGHSFHVDCIDTWFRSRSTCPLCRAPVQPPFQ-------VIETGSSSSSSPLTF 173

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BLAST of Carg04634-RA vs. ExPASy TrEMBL
Match: A0A6J1ESR2 (RING-H2 finger protein ATL40-like OS=Cucurbita moschata OX=3662 GN=LOC111437227 PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 3.2e-119
Identity = 220/221 (99.55%), Postives = 220/221 (99.55%), Query Frame = 0


Query: 1   MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 60
           MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA
Sbjct: 1   MSASSFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHA 60

Query: 61  RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCA 120
           RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLEN AAVECAVCLCA
Sbjct: 61  RRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENPAAVECAVCLCA 120

Query: 121 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS 180
           VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS
Sbjct: 121 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSS 180

Query: 181 SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222
           SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM
Sbjct: 181 SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 221

[Close]

BLAST of Carg04634-RA vs. ExPASy TrEMBL
Match: A0A6J1JP92 (E3 ubiquitin-protein ligase ATL41-like OS=Cucurbita maxima OX=3661 GN=LOC111486268 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.9e-111
Identity = 212/222 (95.50%), Postives = 214/222 (96.40%), Query Frame = 0


Query: 1   MSASSFDAGDSPFWVDHRPNRN-PYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRH 60
           MSASSFDAGDSPFWVDHR NRN PYNLNNRIML+AIISLSLVVLLVIALHIYARYALRRH
Sbjct: 1   MSASSFDAGDSPFWVDHRSNRNHPYNLNNRIMLSAIISLSLVVLLVIALHIYARYALRRH 60

Query: 61  ARRQAVLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLC 120
           ARRQAVLRR GILALV SSDLP PPRHSGLDPLVMASLPVVVFKHQLEN AAVECAVCLC
Sbjct: 61  ARRQAVLRRRGILALVVSSDLPPPPRHSGLDPLVMASLPVVVFKHQLENPAAVECAVCLC 120

Query: 121 AVEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGS 180
           AVE+GEEARVLPNCKHTFHLECIDKWFGLHSTCP CRTEAAPMIRPEPREAPVAESGGGS
Sbjct: 121 AVENGEEARVLPNCKHTFHLECIDKWFGLHSTCPICRTEAAPMIRPEPREAPVAESGGGS 180

Query: 181 SSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222
           SSRLSSF RILSRERSSRRIQPCVRENGNDDVQDLERQQMSM
Sbjct: 181 SSRLSSFGRILSRERSSRRIQPCVRENGNDDVQDLERQQMSM 222

[Close]

BLAST of Carg04634-RA vs. ExPASy TrEMBL
Match: A0A5A7V7D1 (RING-H2 finger protein ATL40-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00200 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.3e-83
Identity = 173/220 (78.64%), Postives = 183/220 (83.18%), Query Frame = 0


Query: 5   SFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQA 64
           SFD GD  F   H P+RNPY+LNN+IMLTAIISLSLVVLLVIALHIYARYALRRHARRQA
Sbjct: 8   SFDDGDIYFGPSH-PHRNPYDLNNKIMLTAIISLSLVVLLVIALHIYARYALRRHARRQA 67

Query: 65  VLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLEN----AAAVECAVCLCA 124
           VLRRLGILALVDSSD   PP  SGLDPLVMAS+PVVVFKHQ EN      AVECAVCL A
Sbjct: 68  VLRRLGILALVDSSDPHLPPSRSGLDPLVMASMPVVVFKHQSENPEPSGGAVECAVCLSA 127

Query: 125 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAP---VAESGG 184
           + DGE AR+LPNCKH FH+ECIDKWFG HSTCP CRTEAAPM+ PEPRE P   V ESGG
Sbjct: 128 IVDGETARILPNCKHVFHVECIDKWFGSHSTCPICRTEAAPMMLPEPREGPAAEVGESGG 187

Query: 185 GSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQ 218
           GSSSRLSSFRRILSRERSS RIQPCV ++ ND V DLE Q
Sbjct: 188 GSSSRLSSFRRILSRERSSLRIQPCVFQHRNDAVPDLESQ 226

[Close]

BLAST of Carg04634-RA vs. ExPASy TrEMBL
Match: A0A1S4DXK5 (RING-H2 finger protein ATL40-like OS=Cucumis melo OX=3656 GN=LOC103491347 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.3e-83
Identity = 173/220 (78.64%), Postives = 183/220 (83.18%), Query Frame = 0


Query: 5   SFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQA 64
           SFD GD  F   H P+RNPY+LNN+IMLTAIISLSLVVLLVIALHIYARYALRRHARRQA
Sbjct: 8   SFDDGDIYFGPSH-PHRNPYDLNNKIMLTAIISLSLVVLLVIALHIYARYALRRHARRQA 67

Query: 65  VLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLEN----AAAVECAVCLCA 124
           VLRRLGILALVDSSD   PP  SGLDPLVMAS+PVVVFKHQ EN      AVECAVCL A
Sbjct: 68  VLRRLGILALVDSSDPHLPPSRSGLDPLVMASMPVVVFKHQSENPEPSGGAVECAVCLSA 127

Query: 125 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAP---VAESGG 184
           + DGE AR+LPNCKH FH+ECIDKWFG HSTCP CRTEAAPM+ PEPRE P   V ESGG
Sbjct: 128 IVDGETARILPNCKHVFHVECIDKWFGSHSTCPICRTEAAPMMLPEPREGPAAEVGESGG 187

Query: 185 GSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQ 218
           GSSSRLSSFRRILSRERSS RIQPCV ++ ND V DLE Q
Sbjct: 188 GSSSRLSSFRRILSRERSSLRIQPCVFQHRNDAVPDLESQ 226

[Close]

BLAST of Carg04634-RA vs. ExPASy TrEMBL
Match: A0A0A0KJL6 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G420520 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.4e-79
Identity = 163/223 (73.09%), Postives = 178/223 (79.82%), Query Frame = 0


Query: 5   SFDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQA 64
           SFD  D  F   H P+RNPY+LNN+IMLTAI+SLSLVV+LVIALHIYARYALRRHARRQA
Sbjct: 8   SFDGDDIYFGPAH-PHRNPYDLNNKIMLTAIVSLSLVVILVIALHIYARYALRRHARRQA 67

Query: 65  VLRRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLE----NAAAVECAVCLCA 124
           VLRRLGILA +DS D   PP  SGLDPLVMAS+PVVVFKHQ E    +   VECAVCL A
Sbjct: 68  VLRRLGILAFIDSGDHHPPPSRSGLDPLVMASMPVVVFKHQSESPDPSGGGVECAVCLSA 127

Query: 125 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAE------ 184
           + DGE AR+LPNCKH FH+ECIDKWFG HSTCP CRTEAAPM+ PEPRE P AE      
Sbjct: 128 IVDGETARILPNCKHVFHVECIDKWFGSHSTCPICRTEAAPMMLPEPREGPAAEVGEGGG 187

Query: 185 SGGGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLERQ 218
            GGGS+SRLSSFRRILSRERSS RIQPCV ++ ND V DLE Q
Sbjct: 188 GGGGSTSRLSSFRRILSRERSSLRIQPCVLQHRNDAVPDLESQ 229

[Close]

BLAST of Carg04634-RA vs. TAIR 10
Match: AT2G42360.1 (RING/U-box superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 2.1e-35
Identity = 103/239 (43.10%), Postives = 134/239 (56.07%), Query Frame = 0


Query: 6   FDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAV 65
           F++    FW    PN + Y+LN++IML A+ SLS V+L+V ALH+YAR+ LRR  RR+A 
Sbjct: 11  FNSDHHSFW----PNPSTYDLNSKIMLAAVASLSGVILIVFALHLYARFVLRR--RREAF 70

Query: 66  LRRLGILALVDSSDLPRPPRHSGLDPLVMASLPV-VVFKHQLENAAAVECAVCLCAVEDG 125
                 L ++       P R  GL+P V+ASLP   V       A+A ECAVCL  +++ 
Sbjct: 71  RG----LPVIFRHPFEMPKR--GLNPTVIASLPTFTVGATDGVAASATECAVCLSVLKEQ 130

Query: 126 EEARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEA--APMIRPEPREAPV---------- 185
           ++AR LPNCKH FH++C+D W    STCP CRTE    P + PEPRE PV          
Sbjct: 131 DKARELPNCKHIFHVDCVDTWLTTCSTCPVCRTEVEPRPRLEPEPREGPVGTAPQLLVET 190

Query: 186 -----AESGGGSS----------SRLSSFRRILSRERSSRRIQPCVRENGNDDVQDLER 217
                 E+   SS          SRL+SFR+IL+RERSS RI     +   D V DLER
Sbjct: 191 RLNLTVEAASSSSSDNKTVVSPASRLNSFRKILTRERSSNRINHSCPD--QDRVADLER 235

[Close]

BLAST of Carg04634-RA vs. TAIR 10
Match: AT2G42350.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 3.1e-34
Identity = 91/230 (39.57%), Postives = 127/230 (55.22%), Query Frame = 0


Query: 7   DAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAVL 66
           D+ D  FW     N   Y+ +++I L   +S S+++++V   ++YA++ L R +  Q   
Sbjct: 9   DSDDRSFW----QNSTSYDASSKIFLVTTVSFSIIIIIVFVYYLYAKFVLHRRSAFQD-- 68

Query: 67  RRLGILALVDSSDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCAVEDGEE 126
                   +  S + +PP+  GLD LV+ASLP  V   +  + A  ECAVCL  +E+ + 
Sbjct: 69  --------LSFSVVSQPPKR-GLDSLVIASLPTFVVGIK-NDVAGTECAVCLSLLEEKDN 128

Query: 127 ARVLPNCKHTFHLECIDKWFGLHSTCPNCRTEAA---PMIRPEPREAPVAE--------- 186
           AR+LPNCKH FH+ C+D W    STCP CRTEA    P + PEPRE PV +         
Sbjct: 129 ARMLPNCKHVFHVSCVDTWLTTQSTCPVCRTEAEPSHPRLEPEPREGPVGDFAPPLDFAG 188

Query: 187 ----SGGGSSSRLSSFRRILSRERSSRRIQPCVRENGNDDVQ---DLERQ 218
               +GG S SRL SFRRIL+RERSS R     R++   D     D+ERQ
Sbjct: 189 VDNKTGGSSVSRLDSFRRILTRERSSNR-----RDHSRVDQDRELDIERQ 217

[Close]

BLAST of Carg04634-RA vs. TAIR 10
Match: AT3G16720.1 (TOXICOS EN LEVADURA 2 )

HSP 1 Score: 115.5 bits (288), Expect = 5.3e-26
Identity = 64/140 (45.71%), Postives = 88/140 (62.86%), Query Frame = 0


Query: 24  YNLNNRIMLTAIISLSLVVLLVIALHIYARY----ALRRHARRQAVLRRLGILALV--DS 83
           Y ++ +IML+AI+ L  VV+L++ LH+YAR+    A RRH RR++  RR  ++      S
Sbjct: 24  YAMSGKIMLSAIVILFFVVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVFFTADPS 83

Query: 84  SDLPRPPRHSGLDPLVMASLPVVVFKHQLENAAAVECAVCLCAVEDGEEARVLPNCKHTF 143
           +         GLDP V+ SLPV  F  +  +   +ECAVCL   E+ E  RVLPNC+HTF
Sbjct: 84  TAATSVVASRGLDPNVIKSLPVFTFSDE-THKDPIECAVCLSEFEESETGRVLPNCQHTF 143

Query: 144 HLECIDKWFGLHSTCPNCRT 158
           H++CID WF  HSTCP CR+
Sbjct: 144 HVDCIDMWFHSHSTCPLCRS 162

[Close]

BLAST of Carg04634-RA vs. TAIR 10
Match: AT3G62690.1 (AtL5 )

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-25
Identity = 71/190 (37.37%), Postives = 102/190 (53.68%), Query Frame = 0


Query: 6   FDAGDSPFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAV 65
           FD+  +  W +     + Y+LN +IML ++I L + V+L++  H YAR+  RR  RR  +
Sbjct: 5   FDSSKT-LWGNMNHGSSRYSLNGKIMLASVIILFVAVILILCFHSYARWLFRRQNRR--I 64

Query: 66  LRRLGILALVDSSDLPRPPRHSG-----LDPLVMASLPVVVFKHQLENAAAVECAVCLCA 125
            RR  I A + S    R P  S      LDP V+  +P+ V+  +   +   EC+VCL  
Sbjct: 65  RRR--ISAHLRSLAAARDPTQSSSSLSPLDPTVLEKIPIFVYSVKTHESPLEECSVCLSE 124

Query: 126 VEDGEEARVLPNCKHTFHLECIDKWFGLHSTCPNCRT---EAAPMIRPEPREA------P 182
            E+ +E RVLP C H FH++CID WF   S+CP CR     A P+  PEP  A      P
Sbjct: 125 FEEDDEGRVLPKCGHVFHVDCIDTWFRSRSSCPLCRAPVQPAQPVTEPEPVAAVFPSVKP 184

[Close]

BLAST of Carg04634-RA vs. TAIR 10
Match: AT2G47560.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-23
Identity = 64/177 (36.16%), Postives = 92/177 (51.98%), Query Frame = 0


Query: 12  PFWVDHRPNRNPYNLNNRIMLTAIISLSLVVLLVIALHIYARYALRRHARRQAVLRRLGI 71
           P W       + Y LN +IML+++I L + V++++  H YAR+  RRH RR  + RR+  
Sbjct: 10  PIWGSVSHTSSGYALNGKIMLSSVIVLFVAVIMILCFHSYARWLFRRHNRR--IRRRI-- 69

Query: 72  LALVDSSDLPRPPRHSGLDPLVMASLPVVVF--KHQLENAAAVECAVCLCAVEDGEEARV 131
                   L   PR   LD  V+  +P+ V+  K+        EC+VCL   E+ +E R+
Sbjct: 70  --RSHLRTLSASPRDQALDQAVLDKIPIFVYSSKNPPPPEEKEECSVCLSEFEEEDEGRL 129

Query: 132 LPNCKHTFHLECIDKWFGLHSTCPNCRTEAAPMIRPEPREAPVAESGGGSSSRLSSF 187
           LP C H+FH++CID WF   STCP CR    P  +       V E+G  SSS   +F
Sbjct: 130 LPKCGHSFHVDCIDTWFRSRSTCPLCRAPVQPPFQ-------VIETGSSSSSSPLTF 173

The following BLAST results are available for this feature:

BLAST of Carg04634-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6589265.1	1.7e-119	100.00	E3 ubiquitin-protein ligase ATL41, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022930874.1	6.5e-119	99.55	RING-H2 finger protein ATL40-like [Cucurbita moschata]	[more]
XP_023529863.1	9.7e-115	95.59	RING-H2 finger protein ATL40-like [Cucurbita pepo subsp. pepo]	[more]
XP_022989093.1	3.8e-111	95.50	E3 ubiquitin-protein ligase ATL41-like [Cucurbita maxima]	[more]
XP_038877602.1	2.3e-87	80.54	RING-H2 finger protein ATL40-like [Benincasa hispida]	[more]

BLAST of Carg04634-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SLC3	3.0e-34	43.10	E3 ubiquitin-protein ligase ATL41 OS=Arabidopsis thaliana OX=3702 GN=ATL41 PE=1 ...	[more]
Q9SLC4	4.3e-33	39.57	RING-H2 finger protein ATL40 OS=Arabidopsis thaliana OX=3702 GN=ATL40 PE=2 SV=1	[more]
Q8L9T5	7.4e-25	45.71	RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2	[more]
Q9LZJ6	1.7e-24	37.37	RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1	[more]
O22255	2.6e-22	36.16	RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1	[more]

BLAST of Carg04634-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ESR2	3.2e-119	99.55	RING-H2 finger protein ATL40-like OS=Cucurbita moschata OX=3662 GN=LOC111437227 ...	[more]
A0A6J1JP92	1.9e-111	95.50	E3 ubiquitin-protein ligase ATL41-like OS=Cucurbita maxima OX=3661 GN=LOC1114862...	[more]
A0A5A7V7D1	1.3e-83	78.64	RING-H2 finger protein ATL40-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S4DXK5	1.3e-83	78.64	RING-H2 finger protein ATL40-like OS=Cucumis melo OX=3656 GN=LOC103491347 PE=4 S...	[more]
A0A0A0KJL6	2.4e-79	73.09	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G420520 P...	[more]

BLAST of Carg04634-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42360.1	2.1e-35	43.10	RING/U-box superfamily protein	[more]
AT2G42350.1	3.1e-34	39.57	RING/U-box superfamily protein	[more]
AT3G16720.1	5.3e-26	45.71	TOXICOS EN LEVADURA 2	[more]
AT3G62690.1	1.2e-25	37.37	AtL5	[more]
AT2G47560.1	1.9e-23	36.16	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 114..155 e-value: 3.6E-6 score: 36.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 113..156 e-value: 2.2E-12 score: 47.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 114..156 score: 12.1057
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 92..162 e-value: 1.1E-20 score: 74.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 201..221
None	No IPR available	PANTHER	PTHR14155:SF558	RING-H2 FINGER PROTEIN ATL40-LIKE	coord: 22..171
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 22..171
None	No IPR available	CDD	cd16461	RING-H2_EL5_like	coord: 113..156 e-value: 7.291E-16 score: 66.9499
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 105..162

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg04634	Carg04634	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04634-RA:1	Carg04634-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04634-RA:cds	Carg04634-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg04634-RA	Carg04634-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane