Carg04548-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04548-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tetraspanin-6-like
Location	Carg_Chr04: 19823060 .. 19824119 (-)
Sequence length	874
RNA-Seq Expression	Carg04548-RA
Synteny	Carg04548-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAAACTAAGCAACTCAGTGATCGGACTCCTGAATTTCCTAACGCTCTTAGCCTCAATCCCCATCATCGGCGGCGCCCTATGGATGGCTCGGAACAGCACGACGTGCGAGGGGTTTCTGCAGACGCCGCTGCTGGTGGTCGGCTTTGTGGTGCTCGTCATTTCGCTGGCGGGGTTCGTGGGGGCCTGTTTCAATGTAGCTTGGGCGCTCTGGCTCTATTTGTTCGTCATGTTGCTGCTCATTGCCACGCTCTTGGGCCTCACAGTTTTTGGGCTTGCTGTGGCCAGTAAAGGCGGCGGCGGCGGCGGCGGCGAGCATGGGCTGGGGGATTACTCGACGTGGCTAAGGAAGAGAGTGGATGATCCTCGGAATTGGGTCACTATTAGAAGCTGTATTTTGGGGTCTAATACTTGCAATCAACTTCCTTCTTGGACTCCTCTTAATTACTTGCAAAGGGACATCACTCCAATTCAGGTACTCCCTTATTTATTTATTTATTTTTTTTTTTTAATTTGGAGTAAAGAAATGATTAGAAAACAAGCACGGTTTTCAAGTAAATTTTGGGAACGCAGTCGGGCTGCTGCAAGCCACCGGCGTCATGCAGCGAGAACGGTCAAGACCCCGATTGCTACCGGTGGAACGGAGCGCCCAACGTGCTGTGCTTCGAGTGCGACTCGTGCGAAGCAGGTGTGCTTGAGACTGCAAGGAAAGACTGGCATAAGCTCTCTATTCTCAACGTTGTGATACTCATTTTTCTCATTGTCGTTTACTCCATCGGTTGTTGTGCGTTCCGAAACGCCAAGCGAGCCGAAGAGAATTATGCATACGGCGAGAATCATATGACCAAAATCGGACCCAGACGAGATTACAAGATGTACGTAATTCATCACTCCTGTTCATTTCATTTTAGTCTCTGTACTTTTAAATATTCGACACTGTTTTATTTCATTTTGTTTCTGCAGGTGGAGATGGTTGGAGGACAGGATGGATCGTTACTAACGGTGGATATGTCATTTTATATTGCTATTTAGTATCGTAAAGTTAGAAAATTAAGCGG

mRNA sequence

ATGAACAAACTAAGCAACTCAGTGATCGGACTCCTGAATTTCCTAACGCTCTTAGCCTCAATCCCCATCATCGGCGGCGCCCTATGGATGGCTCGGAACAGCACGACGTGCGAGGGGTTTCTGCAGACGCCGCTGCTGGTGGTCGGCTTTGTGGTGCTCGTCATTTCGCTGGCGGGGTTCGTGGGGGCCTGTTTCAATGTAGCTTGGGCGCTCTGGCTCTATTTGTTCGTCATGTTGCTGCTCATTGCCACGCTCTTGGGCCTCACAGTTTTTGGGCTTGCTGTGGCCAGTAAAGGCGGCGGCGGCGGCGGCGGCGAGCATGGGCTGGGGGATTACTCGACGTGGCTAAGGAAGAGAGTGGATGATCCTCGGAATTGGGTCACTATTAGAAGCTGTATTTTGGGGTCTAATACTTGCAATCAACTTCCTTCTTGGACTCCTCTTAATTACTTGCAAAGGGACATCACTCCAATTCAGTCGGGCTGCTGCAAGCCACCGGCGTCATGCAGCGAGAACGGTCAAGACCCCGATTGCTACCGGTGGAACGGAGCGCCCAACGTGCTGTGCTTCGAGTGCGACTCGTGCGAAGCAGGTGTGCTTGAGACTGCAAGGAAAGACTGGCATAAGCTCTCTATTCTCAACGTTGTGATACTCATTTTTCTCATTGTCGTTTACTCCATCGGTTGTTGTGCGTTCCGAAACGCCAAGCGAGCCGAAGAGAATTATGCATACGGCGAGAATCATATGACCAAAATCGGACCCAGACGAGATTACAAGATGTGGAGATGGTTGGAGGACAGGATGGATCGTTACTAACGGTGGATATGTCATTTTATATTGCTATTTAGTATCGTAAAGTTAGAAAATTAAGCGG

Coding sequence (CDS)

ATGAACAAACTAAGCAACTCAGTGATCGGACTCCTGAATTTCCTAACGCTCTTAGCCTCAATCCCCATCATCGGCGGCGCCCTATGGATGGCTCGGAACAGCACGACGTGCGAGGGGTTTCTGCAGACGCCGCTGCTGGTGGTCGGCTTTGTGGTGCTCGTCATTTCGCTGGCGGGGTTCGTGGGGGCCTGTTTCAATGTAGCTTGGGCGCTCTGGCTCTATTTGTTCGTCATGTTGCTGCTCATTGCCACGCTCTTGGGCCTCACAGTTTTTGGGCTTGCTGTGGCCAGTAAAGGCGGCGGCGGCGGCGGCGGCGAGCATGGGCTGGGGGATTACTCGACGTGGCTAAGGAAGAGAGTGGATGATCCTCGGAATTGGGTCACTATTAGAAGCTGTATTTTGGGGTCTAATACTTGCAATCAACTTCCTTCTTGGACTCCTCTTAATTACTTGCAAAGGGACATCACTCCAATTCAGTCGGGCTGCTGCAAGCCACCGGCGTCATGCAGCGAGAACGGTCAAGACCCCGATTGCTACCGGTGGAACGGAGCGCCCAACGTGCTGTGCTTCGAGTGCGACTCGTGCGAAGCAGGTGTGCTTGAGACTGCAAGGAAAGACTGGCATAAGCTCTCTATTCTCAACGTTGTGATACTCATTTTTCTCATTGTCGTTTACTCCATCGGTTGTTGTGCGTTCCGAAACGCCAAGCGAGCCGAAGAGAATTATGCATACGGCGAGAATCATATGACCAAAATCGGACCCAGACGAGATTACAAGATGTGGAGATGGTTGGAGGACAGGATGGATCGTTACTAA

Protein sequence

MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGFVGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEENYAYGENHMTKIGPRRDYKMWRWLEDRMDRY

Homology

[Close]

BLAST of Carg04548-RA vs. NCBI nr
Match: KAG7033006.1 (Tetraspanin-6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 560.5 bits (1443), Expect = 8.2e-156
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR
Sbjct: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE
Sbjct: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY
Sbjct: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 271

[Close]

BLAST of Carg04548-RA vs. NCBI nr
Match: KAG6602323.1 (Tetraspanin-6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 547.7 bits (1410), Expect = 5.5e-152
Identity = 265/273 (97.07%), Postives = 268/273 (98.17%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASK--GGGGGGGEHGLGDYSTWLRK 120
           VGACFNVAWALWLYLFVMLLLIATLLGLTVFGL VASK  GGGGGGGEHGLGDYSTWLRK
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLVVASKGGGGGGGGGEHGLGDYSTWLRK 120

Query: 121 RVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDC 180
           RVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPP SCSENGQDPDC
Sbjct: 121 RVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPVSCSENGQDPDC 180

Query: 181 YRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRA 240
           YRWNGAPNVLCFECDSC+AGVLETARKDWHKLS+LNVVILIF IVVYSIGCCAFRNAKRA
Sbjct: 181 YRWNGAPNVLCFECDSCKAGVLETARKDWHKLSVLNVVILIFFIVVYSIGCCAFRNAKRA 240

Query: 241 EENYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           EENYAYGENHMTKIGPRRDYKMW+WLEDRMDRY
Sbjct: 241 EENYAYGENHMTKIGPRRDYKMWKWLEDRMDRY 273

[Close]

BLAST of Carg04548-RA vs. NCBI nr
Match: XP_023548631.1 (tetraspanin-6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.4 bits (1409), Expect = 7.2e-152
Identity = 266/271 (98.15%), Postives = 267/271 (98.52%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNVAWALWLYLFVMLLLIATLLGLTVFGL VASK GGGGGGEHGLGDYSTWLRKRV
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLVVASK-GGGGGGEHGLGDYSTWLRKRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           DDPRNWVTIRSCILGSNTCN LPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR
Sbjct: 121 DDPRNWVTIRSCILGSNTCNHLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLCFECDSC+AGVLETARKDWHKLSILNVVILIF IVVYSIGCCAFRNAKRAEE
Sbjct: 181 WNGAPNVLCFECDSCKAGVLETARKDWHKLSILNVVILIFFIVVYSIGCCAFRNAKRAEE 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY
Sbjct: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 270

[Close]

BLAST of Carg04548-RA vs. NCBI nr
Match: XP_022990440.1 (tetraspanin-6-like [Cucurbita maxima])

HSP 1 Score: 543.9 bits (1400), Expect = 7.9e-151
Identity = 263/271 (97.05%), Postives = 266/271 (98.15%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNVAWALWLYLFVMLLLIATLLGLTVFGL VASKGGGGGGGEHGLGDYSTWLRKRV
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLVVASKGGGGGGGEHGLGDYSTWLRKRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDI+PIQSGCCKPPASCSENGQDPDCYR
Sbjct: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDISPIQSGCCKPPASCSENGQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLC+ECDSC+AGVLETARKDWHKLSILNVVILIF IVVYSIGCCAFRNAKRAEE
Sbjct: 181 WNGAPNVLCYECDSCKAGVLETARKDWHKLSILNVVILIFFIVVYSIGCCAFRNAKRAEE 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           NYAYGEN MTKI PR DYKMWRWLEDRMDRY
Sbjct: 241 NYAYGENRMTKIRPRWDYKMWRWLEDRMDRY 271

[Close]

BLAST of Carg04548-RA vs. NCBI nr
Match: XP_022961606.1 (tetraspanin-6-like [Cucurbita moschata])

HSP 1 Score: 538.5 bits (1386), Expect = 3.3e-149
Identity = 263/271 (97.05%), Postives = 266/271 (98.15%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNVAWALWLYLFVMLLLIATLLGLTVFGL VASK  GGGGGEHGLGDYSTWLRKRV
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLVVASK--GGGGGEHGLGDYSTWLRKRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           DDPRNWVTIRSCILGSNTCNQLPS TPLNYLQRDITPIQSGCCKPPASC+ENGQDPDCYR
Sbjct: 121 DDPRNWVTIRSCILGSNTCNQLPSSTPLNYLQRDITPIQSGCCKPPASCNENGQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLCFECDSC+AGVLETARKDWHKLSILNVVILIF IVVYSIGCCAFRNAKRAEE
Sbjct: 181 WNGAPNVLCFECDSCKAGVLETARKDWHKLSILNVVILIFFIVVYSIGCCAFRNAKRAEE 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           NYAYGENHMTKIGPRRD+KMWRWLEDRMDRY
Sbjct: 241 NYAYGENHMTKIGPRRDHKMWRWLEDRMDRY 269

[Close]

BLAST of Carg04548-RA vs. ExPASy Swiss-Prot
Match: Q9C7C1 (Tetraspanin-6 OS=Arabidopsis thaliana OX=3702 GN=TET6 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.2e-90
Identity = 163/280 (58.21%), Postives = 209/280 (74.64%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           M + SN+VIG+LN LTLLASIPIIG AL+ AR+STTCE FLQTPLLV+GF++L++SLAGF
Sbjct: 1   MYRFSNTVIGVLNLLTLLASIPIIGTALYKARSSTTCENFLQTPLLVIGFIILIVSLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGG-----GGEHGLGDYSTW 120
           +GACFNVAWALW+YL VM+ LIATL+GLT+FGL V S+GGG         E+ LGDY  W
Sbjct: 61  IGACFNVAWALWVYLVVMIFLIATLMGLTLFGLVVTSQGGGVEVPGRIYKEYRLGDYHPW 120

Query: 121 LRKRVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCS-ENG- 180
           LR+RV DP  W +IRSCIL S TC ++ SWT L+Y QRD+T +QSGCCKPP +C+ E G 
Sbjct: 121 LRERVRDPEYWNSIRSCILSSKTCTKIESWTTLDYFQRDMTSVQSGCCKPPTACTYEAGV 180

Query: 181 --QDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCA 240
                DC+RWN    +LC+ECD+C+AGVLE  R DW KLS++N+++L+ LI VY+ GCCA
Sbjct: 181 VDGGGDCFRWNNGVEMLCYECDACKAGVLEEIRLDWRKLSVVNILVLVLLIAVYAAGCCA 240

Query: 241 FRNAKRAEENY-AYGENHMTKIGPRRDYKMWRWLEDRMDR 271
           F N + A   Y    +N MT++ PR DY  WRW  ++ ++
Sbjct: 241 FHNTRHAAHPYHPSDDNRMTRVRPRWDYYWWRWWHEKKEQ 280

[Close]

BLAST of Carg04548-RA vs. ExPASy Swiss-Prot
Match: Q84WF6 (Tetraspanin-5 OS=Arabidopsis thaliana OX=3702 GN=TET5 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.6e-85
Identity = 155/282 (54.96%), Postives = 201/282 (71.28%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MN++SN+VIG LN LTL++SI ++G ALWM R+ TTCE FLQ PLL++G  +L++S+AG 
Sbjct: 1   MNRMSNTVIGFLNILTLISSIVLLGSALWMGRSKTTCEHFLQKPLLILGLAILILSVAGL 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGG-----GGGGGEHGLGDYSTW 120
           VGAC +VAW LW+YLF M+ +I  L+GLT+FG  V S  G     G    E  L  Y  W
Sbjct: 61  VGACCDVAWVLWVYLFFMVFIIVALMGLTLFGFIVTSHSGGVVVDGRVYKEFKLEAYHPW 120

Query: 121 LRKRVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSEN--- 180
           L+ RV D   WVTI++C+LGS TC++L  WTPL+YLQ+D++P+QSGCCKPP SC  N   
Sbjct: 121 LKTRVVDTNYWVTIKTCLLGSVTCSKLALWTPLDYLQKDLSPLQSGCCKPPTSCVYNTDT 180

Query: 181 --GQDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCC 240
              QDPDCYRWN A  VLC++CD+C AGVLET R+DWHKLS++NV+++IFLI VY +GCC
Sbjct: 181 VIQQDPDCYRWNNAATVLCYDCDTCRAGVLETVRRDWHKLSLVNVIVVIFLIAVYCVGCC 240

Query: 241 AFRNAKRAEE-NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           AF+NAKR +   + YG   M+K  P  +    RW   R DRY
Sbjct: 241 AFKNAKRPQHYGFPYGRYGMSKSRPGWEQSWSRWWHGR-DRY 281

[Close]

BLAST of Carg04548-RA vs. ExPASy Swiss-Prot
Match: Q8S8Q6 (Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 5.1e-60
Identity = 116/267 (43.45%), Postives = 174/267 (65.17%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMA-RNSTTCEGFLQTPLLVVGFVVLVISLAG 60
           M + SN+++G+LNFL  L SIPI+ G +W++ + ST CE FL  P++ +G  ++V+++AG
Sbjct: 1   MARCSNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAG 60

Query: 61  FVGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGG-----GGGGGEHGLGDYST 120
            +G+C  V W LW+YLFVM LLI  +  +TVF   V +KG      G G  E+ LGDYST
Sbjct: 61  LIGSCCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYST 120

Query: 121 WLRKRVDDPRNWVTIRSCILGSNTCNQLPSW---TPLN-YLQRDITPIQSGCCKPPASC- 180
           WL+KRV++ +NW  IRSC++ S  C++L +     P+N + +  +T +QSGCCKP   C 
Sbjct: 121 WLQKRVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECG 180

Query: 181 -------------SENGQDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVV 240
                        +    +PDC  W+ A   LCF+C SC+AG+L+  +  W K++I+N+V
Sbjct: 181 FEYVNPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIV 240

Query: 241 ILIFLIVVYSIGCCAFRNAKRAEENYA 244
            L+FLI+VYS+GCCAFRN KR +++Y+
Sbjct: 241 FLVFLIIVYSVGCCAFRNNKR-DDSYS 266

[Close]

BLAST of Carg04548-RA vs. ExPASy Swiss-Prot
Match: Q9M1E7 (Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.4e-57
Identity = 121/280 (43.21%), Postives = 170/280 (60.71%), Query Frame = 0


Query: 3   KLSNSVIGLLNFLTLLASIPIIGGALWMA--RNSTTCEGFLQTPLLVVGFVVLVISLAGF 62
           + SN +IGL+NFLT L SIPI+GG +W++   NST C  FLQ PL+V+G  ++V+SLAGF
Sbjct: 2   RTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAGF 61

Query: 63  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGG-----GGGGEHGLGDYSTW 122
            GAC+   + +WLYL VMLL+IA L+G  +F  AV  KG G      G  ++ L DYS W
Sbjct: 62  AGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSGW 121

Query: 123 LRKRVDDPRNWVTIRSCILGSNTC-------NQLPSWTPLNYLQRDITPIQSGCCKPPAS 182
           L+ RV D   W  I SC+  S  C       N +P    + +L+R ++P++SGCCKPP  
Sbjct: 122 LKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRR-LSPVESGCCKPPTD 181

Query: 183 C-----SENGQD---------PDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILN 242
           C     +E G D          DC  W+   ++LC++C SC+AGVL + +K W K+S++N
Sbjct: 182 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 241

Query: 243 VVILIFLIVVYSIGCCAFRNAKRAEENYAYGENHMTKIGP 255
           +V+LI L++ Y I   A+RN KR + +   GE  MTK  P
Sbjct: 242 IVVLIILVIFYVIAYAAYRNVKRIDNDEPAGEARMTKSHP 280

[Close]

BLAST of Carg04548-RA vs. ExPASy Swiss-Prot
Match: Q9LSS4 (Tetraspanin-4 OS=Arabidopsis thaliana OX=3702 GN=TET4 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.1e-54
Identity = 111/275 (40.36%), Postives = 160/275 (58.18%), Query Frame = 0


Query: 6   NSVIGLLNFLTLLASIPIIGGALWMA--RNSTTCEGFLQTPLLVVGFVVLVISLAGFVGA 65
           +++IGL+NF T L SIPI+GG +W++   NST C  FLQ PL+++G  ++VISLAG  GA
Sbjct: 5   SNLIGLINFFTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIIIGISIMVISLAGIAGA 64

Query: 66  CFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGG-----GGGGEHGLGDYSTWLRK 125
           C+   + +WLYLF M  +IA L+G T+F   V  KG G         ++ L DYS WL+ 
Sbjct: 65  CYQNKFLMWLYLFTMFFVIAALIGFTIFAYVVTDKGSGRFVMNRRYLDYYLNDYSGWLKD 124

Query: 126 RVDDPRNWVTIRSCILGSNTC-------NQLPSWTPLNYLQRDITPIQSGCCKPPASCSE 185
           RV D   W  I SC+  S  C       N +P    + Y  R+++P++SGCCKPP  C  
Sbjct: 125 RVTDNGYWRDIGSCVRDSGVCKKIGRDLNGVPETAHMFYF-RNLSPVESGCCKPPTDCGY 184

Query: 186 N--------------GQDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVI 245
                          G +PDC  WN    +LC++C SC+AGVL + +K W K+S++N+V+
Sbjct: 185 TYVNETVWIPGGEMVGPNPDCMLWNNDQRLLCYQCSSCKAGVLGSLKKSWRKVSVINIVV 244

Query: 246 LIFLIVVYSIGCCAFRNAKRAEENYAYGENHMTKI 253
           +I L++ Y I C A++N KR   +   GE  MT +
Sbjct: 245 VIILVIFYVIACAAYQNVKRMYNDEPVGEARMTNL 278

[Close]

BLAST of Carg04548-RA vs. ExPASy TrEMBL
Match: A0A6J1JQ40 (tetraspanin-6-like OS=Cucurbita maxima OX=3661 GN=LOC111487296 PE=4 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 3.8e-151
Identity = 263/271 (97.05%), Postives = 266/271 (98.15%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNVAWALWLYLFVMLLLIATLLGLTVFGL VASKGGGGGGGEHGLGDYSTWLRKRV
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLVVASKGGGGGGGEHGLGDYSTWLRKRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDI+PIQSGCCKPPASCSENGQDPDCYR
Sbjct: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDISPIQSGCCKPPASCSENGQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLC+ECDSC+AGVLETARKDWHKLSILNVVILIF IVVYSIGCCAFRNAKRAEE
Sbjct: 181 WNGAPNVLCYECDSCKAGVLETARKDWHKLSILNVVILIFFIVVYSIGCCAFRNAKRAEE 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           NYAYGEN MTKI PR DYKMWRWLEDRMDRY
Sbjct: 241 NYAYGENRMTKIRPRWDYKMWRWLEDRMDRY 271

[Close]

BLAST of Carg04548-RA vs. ExPASy TrEMBL
Match: A0A6J1HAU2 (tetraspanin-6-like OS=Cucurbita moschata OX=3662 GN=LOC111462197 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.6e-149
Identity = 263/271 (97.05%), Postives = 266/271 (98.15%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNVAWALWLYLFVMLLLIATLLGLTVFGL VASK  GGGGGEHGLGDYSTWLRKRV
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLVVASK--GGGGGEHGLGDYSTWLRKRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           DDPRNWVTIRSCILGSNTCNQLPS TPLNYLQRDITPIQSGCCKPPASC+ENGQDPDCYR
Sbjct: 121 DDPRNWVTIRSCILGSNTCNQLPSSTPLNYLQRDITPIQSGCCKPPASCNENGQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLCFECDSC+AGVLETARKDWHKLSILNVVILIF IVVYSIGCCAFRNAKRAEE
Sbjct: 181 WNGAPNVLCFECDSCKAGVLETARKDWHKLSILNVVILIFFIVVYSIGCCAFRNAKRAEE 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           NYAYGENHMTKIGPRRD+KMWRWLEDRMDRY
Sbjct: 241 NYAYGENHMTKIGPRRDHKMWRWLEDRMDRY 269

[Close]

BLAST of Carg04548-RA vs. ExPASy TrEMBL
Match: A0A6J1BYK5 (tetraspanin-6-like OS=Momordica charantia OX=3673 GN=LOC111006441 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.7e-133
Identity = 233/270 (86.30%), Postives = 249/270 (92.22%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNK SN+VIGLLN LTL+ASIPIIGGALWMARNSTTCEGFLQTPLLV+GFVVL+ISL GF
Sbjct: 1   MNKFSNTVIGLLNLLTLIASIPIIGGALWMARNSTTCEGFLQTPLLVLGFVVLLISLTGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNVAWALWLYLFVMLLLIATLLGLT+FGL VASK GGG GGEHGLGDYS WLR RV
Sbjct: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTLFGLVVASK-GGGSGGEHGLGDYSEWLRHRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           DDPRNW+ IRSCILGSNTCN+L SWTPLNYLQRDITPIQSGCC+PPASCSEN QDPDCYR
Sbjct: 121 DDPRNWMAIRSCILGSNTCNKLSSWTPLNYLQRDITPIQSGCCRPPASCSENAQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLC+ECDSC+AGVLETAR DW KLS+LNVV+LIFLI VYSIGCCAFRN KRA  
Sbjct: 181 WNGAPNVLCYECDSCKAGVLETARGDWRKLSVLNVVMLIFLIGVYSIGCCAFRNTKRAGA 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDR 271
           +Y+YGEN MTKI PR DYKMWRWLEDR ++
Sbjct: 241 DYSYGENRMTKIQPRWDYKMWRWLEDRKEQ 269

[Close]

BLAST of Carg04548-RA vs. ExPASy TrEMBL
Match: A0A6J1FM74 (tetraspanin-6-like OS=Cucurbita moschata OX=3662 GN=LOC111445479 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.0e-127
Identity = 221/271 (81.55%), Postives = 244/271 (90.04%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNSVIGLLNFLTLL SIPIIGGALWMARNSTTCE FLQTPLLV+GF+VL+ISLAGF
Sbjct: 1   MNKLSNSVIGLLNFLTLLGSIPIIGGALWMARNSTTCEAFLQTPLLVLGFLVLLISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACFNV WALWLYLF MLLLIATLLG T+FGL VASKGGGG GGEH  GDYS WLR RV
Sbjct: 61  VGACFNVTWALWLYLFAMLLLIATLLGFTLFGLIVASKGGGGDGGEHRFGDYSVWLRNRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCYR 180
           ++P+ WVTI+SCILGSNTCNQ+ SWTPLNYLQR ITPIQ+GCCKPPASC+EN QDPDCYR
Sbjct: 121 NNPQYWVTIKSCILGSNTCNQVSSWTPLNYLQRQITPIQAGCCKPPASCTENVQDPDCYR 180

Query: 181 WNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAEE 240
           WNGAPNVLC++C SC+AGVLETAR+DWHKLS+LN+V+LIFLI VYSIGCCAFRN KRA+ 
Sbjct: 181 WNGAPNVLCYDCISCKAGVLETARRDWHKLSVLNIVMLIFLIAVYSIGCCAFRNTKRAQT 240

Query: 241 NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           +YA+GEN M KI P  D+KM RWLEDR + Y
Sbjct: 241 DYAHGENRMIKIQPTWDHKMRRWLEDRKELY 271

[Close]

BLAST of Carg04548-RA vs. ExPASy TrEMBL
Match: A0A0A0KNJ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G492320 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.8e-125
Identity = 221/272 (81.25%), Postives = 245/272 (90.07%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MNKLSNS+IGLLN LTLL SIPIIGGALWMARNSTTCE FLQ PLLVVGF+VL+ISLAGF
Sbjct: 1   MNKLSNSLIGLLNLLTLLGSIPIIGGALWMARNSTTCEAFLQRPLLVVGFLVLLISLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGGGGEHGLGDYSTWLRKRV 120
           VGACF+VAWALWLYLFVMLLLIATLLGLT+FG+ VAS    GGGGEH +GDYS WLR RV
Sbjct: 61  VGACFDVAWALWLYLFVMLLLIATLLGLTIFGIVVAS----GGGGEHRVGDYSAWLRNRV 120

Query: 121 DDPRNWVTIRSCILGSNTCNQLP-SWTPLNYLQRDITPIQSGCCKPPASCSENGQDPDCY 180
           ++P+ W+TIRSCILGSNTCNQ   S++PLNYLQRDITPIQSGCCKPP++CSEN QDPDCY
Sbjct: 121 NNPQYWITIRSCILGSNTCNQASNSFSPLNYLQRDITPIQSGCCKPPSTCSENVQDPDCY 180

Query: 181 RWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCAFRNAKRAE 240
           RWNGAPN+LC++CDSC+  VLETAR+DWHKLSILNVV+LIFLIV+YSIGCCAFRN KRA 
Sbjct: 181 RWNGAPNILCYDCDSCKVAVLETARRDWHKLSILNVVMLIFLIVIYSIGCCAFRNTKRAR 240

Query: 241 ENYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
            +YAYGEN MTKI PR DYKMWRWLEDR + Y
Sbjct: 241 ADYAYGENRMTKIQPRWDYKMWRWLEDRKEFY 268

[Close]

BLAST of Carg04548-RA vs. TAIR 10
Match: AT3G12090.1 (tetraspanin6 )

HSP 1 Score: 334.3 bits (856), Expect = 8.9e-92
Identity = 163/280 (58.21%), Postives = 209/280 (74.64%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           M + SN+VIG+LN LTLLASIPIIG AL+ AR+STTCE FLQTPLLV+GF++L++SLAGF
Sbjct: 1   MYRFSNTVIGVLNLLTLLASIPIIGTALYKARSSTTCENFLQTPLLVIGFIILIVSLAGF 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGGGG-----GGEHGLGDYSTW 120
           +GACFNVAWALW+YL VM+ LIATL+GLT+FGL V S+GGG         E+ LGDY  W
Sbjct: 61  IGACFNVAWALWVYLVVMIFLIATLMGLTLFGLVVTSQGGGVEVPGRIYKEYRLGDYHPW 120

Query: 121 LRKRVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCS-ENG- 180
           LR+RV DP  W +IRSCIL S TC ++ SWT L+Y QRD+T +QSGCCKPP +C+ E G 
Sbjct: 121 LRERVRDPEYWNSIRSCILSSKTCTKIESWTTLDYFQRDMTSVQSGCCKPPTACTYEAGV 180

Query: 181 --QDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCCA 240
                DC+RWN    +LC+ECD+C+AGVLE  R DW KLS++N+++L+ LI VY+ GCCA
Sbjct: 181 VDGGGDCFRWNNGVEMLCYECDACKAGVLEEIRLDWRKLSVVNILVLVLLIAVYAAGCCA 240

Query: 241 FRNAKRAEENY-AYGENHMTKIGPRRDYKMWRWLEDRMDR 271
           F N + A   Y    +N MT++ PR DY  WRW  ++ ++
Sbjct: 241 FHNTRHAAHPYHPSDDNRMTRVRPRWDYYWWRWWHEKKEQ 280

[Close]

BLAST of Carg04548-RA vs. TAIR 10
Match: AT4G23410.1 (tetraspanin5 )

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-86
Identity = 155/282 (54.96%), Postives = 201/282 (71.28%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMARNSTTCEGFLQTPLLVVGFVVLVISLAGF 60
           MN++SN+VIG LN LTL++SI ++G ALWM R+ TTCE FLQ PLL++G  +L++S+AG 
Sbjct: 1   MNRMSNTVIGFLNILTLISSIVLLGSALWMGRSKTTCEHFLQKPLLILGLAILILSVAGL 60

Query: 61  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGG-----GGGGGEHGLGDYSTW 120
           VGAC +VAW LW+YLF M+ +I  L+GLT+FG  V S  G     G    E  L  Y  W
Sbjct: 61  VGACCDVAWVLWVYLFFMVFIIVALMGLTLFGFIVTSHSGGVVVDGRVYKEFKLEAYHPW 120

Query: 121 LRKRVDDPRNWVTIRSCILGSNTCNQLPSWTPLNYLQRDITPIQSGCCKPPASCSEN--- 180
           L+ RV D   WVTI++C+LGS TC++L  WTPL+YLQ+D++P+QSGCCKPP SC  N   
Sbjct: 121 LKTRVVDTNYWVTIKTCLLGSVTCSKLALWTPLDYLQKDLSPLQSGCCKPPTSCVYNTDT 180

Query: 181 --GQDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVILIFLIVVYSIGCC 240
              QDPDCYRWN A  VLC++CD+C AGVLET R+DWHKLS++NV+++IFLI VY +GCC
Sbjct: 181 VIQQDPDCYRWNNAATVLCYDCDTCRAGVLETVRRDWHKLSLVNVIVVIFLIAVYCVGCC 240

Query: 241 AFRNAKRAEE-NYAYGENHMTKIGPRRDYKMWRWLEDRMDRY 272
           AF+NAKR +   + YG   M+K  P  +    RW   R DRY
Sbjct: 241 AFKNAKRPQHYGFPYGRYGMSKSRPGWEQSWSRWWHGR-DRY 281

[Close]

BLAST of Carg04548-RA vs. TAIR 10
Match: AT2G23810.1 (tetraspanin8 )

HSP 1 Score: 232.6 bits (592), Expect = 3.6e-61
Identity = 116/267 (43.45%), Postives = 174/267 (65.17%), Query Frame = 0


Query: 1   MNKLSNSVIGLLNFLTLLASIPIIGGALWMA-RNSTTCEGFLQTPLLVVGFVVLVISLAG 60
           M + SN+++G+LNFL  L SIPI+ G +W++ + ST CE FL  P++ +G  ++V+++AG
Sbjct: 1   MARCSNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAG 60

Query: 61  FVGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGG-----GGGGGEHGLGDYST 120
            +G+C  V W LW+YLFVM LLI  +  +TVF   V +KG      G G  E+ LGDYST
Sbjct: 61  LIGSCCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYST 120

Query: 121 WLRKRVDDPRNWVTIRSCILGSNTCNQLPSW---TPLN-YLQRDITPIQSGCCKPPASC- 180
           WL+KRV++ +NW  IRSC++ S  C++L +     P+N + +  +T +QSGCCKP   C 
Sbjct: 121 WLQKRVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECG 180

Query: 181 -------------SENGQDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVV 240
                        +    +PDC  W+ A   LCF+C SC+AG+L+  +  W K++I+N+V
Sbjct: 181 FEYVNPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIV 240

Query: 241 ILIFLIVVYSIGCCAFRNAKRAEENYA 244
            L+FLI+VYS+GCCAFRN KR +++Y+
Sbjct: 241 FLVFLIIVYSVGCCAFRNNKR-DDSYS 266

[Close]

BLAST of Carg04548-RA vs. TAIR 10
Match: AT3G45600.1 (tetraspanin3 )

HSP 1 Score: 224.6 bits (571), Expect = 9.9e-59
Identity = 121/280 (43.21%), Postives = 170/280 (60.71%), Query Frame = 0


Query: 3   KLSNSVIGLLNFLTLLASIPIIGGALWMA--RNSTTCEGFLQTPLLVVGFVVLVISLAGF 62
           + SN +IGL+NFLT L SIPI+GG +W++   NST C  FLQ PL+V+G  ++V+SLAGF
Sbjct: 2   RTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAGF 61

Query: 63  VGACFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGG-----GGGGEHGLGDYSTW 122
            GAC+   + +WLYL VMLL+IA L+G  +F  AV  KG G      G  ++ L DYS W
Sbjct: 62  AGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSGW 121

Query: 123 LRKRVDDPRNWVTIRSCILGSNTC-------NQLPSWTPLNYLQRDITPIQSGCCKPPAS 182
           L+ RV D   W  I SC+  S  C       N +P    + +L+R ++P++SGCCKPP  
Sbjct: 122 LKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRR-LSPVESGCCKPPTD 181

Query: 183 C-----SENGQD---------PDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILN 242
           C     +E G D          DC  W+   ++LC++C SC+AGVL + +K W K+S++N
Sbjct: 182 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 241

Query: 243 VVILIFLIVVYSIGCCAFRNAKRAEENYAYGENHMTKIGP 255
           +V+LI L++ Y I   A+RN KR + +   GE  MTK  P
Sbjct: 242 IVVLIILVIFYVIAYAAYRNVKRIDNDEPAGEARMTKSHP 280

[Close]

BLAST of Carg04548-RA vs. TAIR 10
Match: AT5G60220.1 (tetraspanin4 )

HSP 1 Score: 214.9 bits (546), Expect = 7.8e-56
Identity = 111/275 (40.36%), Postives = 160/275 (58.18%), Query Frame = 0


Query: 6   NSVIGLLNFLTLLASIPIIGGALWMA--RNSTTCEGFLQTPLLVVGFVVLVISLAGFVGA 65
           +++IGL+NF T L SIPI+GG +W++   NST C  FLQ PL+++G  ++VISLAG  GA
Sbjct: 5   SNLIGLINFFTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIIIGISIMVISLAGIAGA 64

Query: 66  CFNVAWALWLYLFVMLLLIATLLGLTVFGLAVASKGGG-----GGGGEHGLGDYSTWLRK 125
           C+   + +WLYLF M  +IA L+G T+F   V  KG G         ++ L DYS WL+ 
Sbjct: 65  CYQNKFLMWLYLFTMFFVIAALIGFTIFAYVVTDKGSGRFVMNRRYLDYYLNDYSGWLKD 124

Query: 126 RVDDPRNWVTIRSCILGSNTC-------NQLPSWTPLNYLQRDITPIQSGCCKPPASCSE 185
           RV D   W  I SC+  S  C       N +P    + Y  R+++P++SGCCKPP  C  
Sbjct: 125 RVTDNGYWRDIGSCVRDSGVCKKIGRDLNGVPETAHMFYF-RNLSPVESGCCKPPTDCGY 184

Query: 186 N--------------GQDPDCYRWNGAPNVLCFECDSCEAGVLETARKDWHKLSILNVVI 245
                          G +PDC  WN    +LC++C SC+AGVL + +K W K+S++N+V+
Sbjct: 185 TYVNETVWIPGGEMVGPNPDCMLWNNDQRLLCYQCSSCKAGVLGSLKKSWRKVSVINIVV 244

Query: 246 LIFLIVVYSIGCCAFRNAKRAEENYAYGENHMTKI 253
           +I L++ Y I C A++N KR   +   GE  MT +
Sbjct: 245 VIILVIFYVIACAAYQNVKRMYNDEPVGEARMTNL 278

The following BLAST results are available for this feature:

BLAST of Carg04548-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033006.1	8.2e-156	100.00	Tetraspanin-6, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6602323.1	5.5e-152	97.07	Tetraspanin-6, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023548631.1	7.2e-152	98.15	tetraspanin-6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022990440.1	7.9e-151	97.05	tetraspanin-6-like [Cucurbita maxima]	[more]
XP_022961606.1	3.3e-149	97.05	tetraspanin-6-like [Cucurbita moschata]	[more]

BLAST of Carg04548-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7C1	1.2e-90	58.21	Tetraspanin-6 OS=Arabidopsis thaliana OX=3702 GN=TET6 PE=2 SV=1	[more]
Q84WF6	1.6e-85	54.96	Tetraspanin-5 OS=Arabidopsis thaliana OX=3702 GN=TET5 PE=2 SV=1	[more]
Q8S8Q6	5.1e-60	43.45	Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1	[more]
Q9M1E7	1.4e-57	43.21	Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1	[more]
Q9LSS4	1.1e-54	40.36	Tetraspanin-4 OS=Arabidopsis thaliana OX=3702 GN=TET4 PE=3 SV=1	[more]

BLAST of Carg04548-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JQ40	3.8e-151	97.05	tetraspanin-6-like OS=Cucurbita maxima OX=3661 GN=LOC111487296 PE=4 SV=1	[more]
A0A6J1HAU2	1.6e-149	97.05	tetraspanin-6-like OS=Cucurbita moschata OX=3662 GN=LOC111462197 PE=4 SV=1	[more]
A0A6J1BYK5	4.7e-133	86.30	tetraspanin-6-like OS=Momordica charantia OX=3673 GN=LOC111006441 PE=4 SV=1	[more]
A0A6J1FM74	5.0e-127	81.55	tetraspanin-6-like OS=Cucurbita moschata OX=3662 GN=LOC111445479 PE=4 SV=1	[more]
A0A0A0KNJ2	2.8e-125	81.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G492320 PE=4 SV=1	[more]

BLAST of Carg04548-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12090.1	8.9e-92	58.21	tetraspanin6	[more]
AT4G23410.1	1.1e-86	54.96	tetraspanin5	[more]
AT2G23810.1	3.6e-61	43.45	tetraspanin8	[more]
AT3G45600.1	9.9e-59	43.21	tetraspanin3	[more]
AT5G60220.1	7.8e-56	40.36	tetraspanin4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018499	Tetraspanin/Peripherin	PFAM	PF00335	Tetraspanin	coord: 8..231 e-value: 1.5E-27 score: 96.8
IPR044991	Tetraspani, plant	PANTHER	PTHR32191	TETRASPANIN-8-RELATED	coord: 1..263
None	No IPR available	PANTHER	PTHR32191:SF40	TETRASPANIN/PERIPHERIN-RELATED	coord: 1..263

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg04548	Carg04548	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04548-RA:3	Carg04548-RA:3	exon
Carg04548-RA:2	Carg04548-RA:2	exon
Carg04548-RA:1	Carg04548-RA:1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04548-RA:three_prime_utr	Carg04548-RA:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04548-RA:cds	Carg04548-RA:cds_3	CDS
Carg04548-RA:cds	Carg04548-RA:cds_2	CDS
Carg04548-RA:cds	Carg04548-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg04548-RA	Carg04548-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009734	auxin-activated signaling pathway
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009506	plasmodesma