Carg03654-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03654-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionF-box protein
LocationCarg_Chr14: 1443578 .. 1444672 (-)
Sequence length1095
RNA-Seq ExpressionCarg03654-RA
SyntenyCarg03654-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATTTTGTACCATTGGGATTTCAGGCCCGGAGCTATATCGGACCTCTTCCCCGTTGGCGTCGTCGGCGTTCTCCTCCGGTGACGGTGACGCCGCTACTACGTCTGTTACCGATATCCATCCAGATGTTCTCCGAACTCACATCTTGACGCGTCTCGATGGACCAGCACTTGCCGCGGTCGCCTCAACTTCGTCGCAACTTCACTGCCTCACTGCCGACACAAACCTCTGGGCGGAACTTTGCCGCTCCACGTGGCCGTCGACGGCGACACAGCGCCTCCACGCTCTGATTTCAGAATTTCCCAACGGTCCTAGGTCCTTCTTCTCTGATTCCTTCCCTCGTCTTTCTTATCACTCCGAAACTTCAACCTCTTCTTCTCCGTCCTCGGCAACCTACAGCCGCCGCAGCCGCCGACGCCACCACTTTACGCCGGAATTGATCTCCGCCGTCGACATCTACTACCGGGGAGAGATGGTTTTCAGCAAAGTCGTCGAGACAGAGACCCTCTCCGGATGGTTCCGATGCACGCCGTTTCGAATCGATATGCTTGATCCTAAGGACGTAGTTCAAACGCCGATTAAATTTCCAATCGGCGACGGAAATACATGTTGGGAGCTTACGGAGGATCTGACTCTGAGCTGGATCATGATCGACACAGCCGGACGGCGAGCGATGAATCTGTCGAGCTACCGGCCGGTCAGCGTGCAGCGACACTGGTTGAGCGGGGAGGTGCACGTAAGATTCGCAGCCGTGCTCGACGGTGAAAGAGGAACGGCGATGGAATGCGTGCAGTGCGGGATTGCAGTGACGTGCGGAGGATGGGAAGGAGGGAATTTACAGGTGAGGGAAGTGAATTTGCAGGTGGAGGATCTGGACGGAACATATTTGAATGGGAAGGATAGTTTAGTAATTTTGGAGAGGGCGTTGGAGGGTAAAACGGGCAGAAGGGGAAGGAAAAAGTTGGAGGAGTTTATGGCGAAGAAAAGGGAGAGGAAAGAAAGGACGTTGAGGAGAGAAGGGACGTTAGATATGGTGTGTTTGGCTTTTGGGTTTCTTGGGTTTGCTACTTTCCTTCTTTGTCTTATTATTTAA

mRNA sequence

ATGTCATTTTGTACCATTGGGATTTCAGGCCCGGAGCTATATCGGACCTCTTCCCCGTTGGCGTCGTCGGCGTTCTCCTCCGGTGACGGTGACGCCGCTACTACGTCTGTTACCGATATCCATCCAGATGTTCTCCGAACTCACATCTTGACGCGTCTCGATGGACCAGCACTTGCCGCGGTCGCCTCAACTTCGTCGCAACTTCACTGCCTCACTGCCGACACAAACCTCTGGGCGGAACTTTGCCGCTCCACGTGGCCGTCGACGGCGACACAGCGCCTCCACGCTCTGATTTCAGAATTTCCCAACGGTCCTAGGTCCTTCTTCTCTGATTCCTTCCCTCGTCTTTCTTATCACTCCGAAACTTCAACCTCTTCTTCTCCGTCCTCGGCAACCTACAGCCGCCGCAGCCGCCGACGCCACCACTTTACGCCGGAATTGATCTCCGCCGTCGACATCTACTACCGGGGAGAGATGGTTTTCAGCAAAGTCGTCGAGACAGAGACCCTCTCCGGATGGTTCCGATGCACGCCGTTTCGAATCGATATGCTTGATCCTAAGGACGTAGTTCAAACGCCGATTAAATTTCCAATCGGCGACGGAAATACATGTTGGGAGCTTACGGAGGATCTGACTCTGAGCTGGATCATGATCGACACAGCCGGACGGCGAGCGATGAATCTGTCGAGCTACCGGCCGGTCAGCGTGCAGCGACACTGGTTGAGCGGGGAGGTGCACGTAAGATTCGCAGCCGTGCTCGACGGTGAAAGAGGAACGGCGATGGAATGCGTGCAGTGCGGGATTGCAGTGACGTGCGGAGGATGGGAAGGAGGGAATTTACAGGTGAGGGAAGTGAATTTGCAGGTGGAGGATCTGGACGGAACATATTTGAATGGGAAGGATAGTTTAGTAATTTTGGAGAGGGCGTTGGAGGGTAAAACGGGCAGAAGGGGAAGGAAAAAGTTGGAGGAGTTTATGGCGAAGAAAAGGGAGAGGAAAGAAAGGACGTTGAGGAGAGAAGGGACGTTAGATATGGTGTGTTTGGCTTTTGGGTTTCTTGGGTTTGCTACTTTCCTTCTTTGTCTTATTATTTAA

Coding sequence (CDS)

ATGTCATTTTGTACCATTGGGATTTCAGGCCCGGAGCTATATCGGACCTCTTCCCCGTTGGCGTCGTCGGCGTTCTCCTCCGGTGACGGTGACGCCGCTACTACGTCTGTTACCGATATCCATCCAGATGTTCTCCGAACTCACATCTTGACGCGTCTCGATGGACCAGCACTTGCCGCGGTCGCCTCAACTTCGTCGCAACTTCACTGCCTCACTGCCGACACAAACCTCTGGGCGGAACTTTGCCGCTCCACGTGGCCGTCGACGGCGACACAGCGCCTCCACGCTCTGATTTCAGAATTTCCCAACGGTCCTAGGTCCTTCTTCTCTGATTCCTTCCCTCGTCTTTCTTATCACTCCGAAACTTCAACCTCTTCTTCTCCGTCCTCGGCAACCTACAGCCGCCGCAGCCGCCGACGCCACCACTTTACGCCGGAATTGATCTCCGCCGTCGACATCTACTACCGGGGAGAGATGGTTTTCAGCAAAGTCGTCGAGACAGAGACCCTCTCCGGATGGTTCCGATGCACGCCGTTTCGAATCGATATGCTTGATCCTAAGGACGTAGTTCAAACGCCGATTAAATTTCCAATCGGCGACGGAAATACATGTTGGGAGCTTACGGAGGATCTGACTCTGAGCTGGATCATGATCGACACAGCCGGACGGCGAGCGATGAATCTGTCGAGCTACCGGCCGGTCAGCGTGCAGCGACACTGGTTGAGCGGGGAGGTGCACGTAAGATTCGCAGCCGTGCTCGACGGTGAAAGAGGAACGGCGATGGAATGCGTGCAGTGCGGGATTGCAGTGACGTGCGGAGGATGGGAAGGAGGGAATTTACAGGTGAGGGAAGTGAATTTGCAGGTGGAGGATCTGGACGGAACATATTTGAATGGGAAGGATAGTTTAGTAATTTTGGAGAGGGCGTTGGAGGGTAAAACGGGCAGAAGGGGAAGGAAAAAGTTGGAGGAGTTTATGGCGAAGAAAAGGGAGAGGAAAGAAAGGACGTTGAGGAGAGAAGGGACGTTAGATATGGTGTGTTTGGCTTTTGGGTTTCTTGGGTTTGCTACTTTCCTTCTTTGTCTTATTATTTAA

Protein sequence

MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLLCLII
Homology
BLAST of Carg03654-RA vs. NCBI nr
Match: XP_022935408.1 (probable F-box protein At2g36090 [Cucurbita moschata] >KAG7017474.1 putative F-box protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 726.9 bits (1875), Expect = 8.8e-206
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60
           MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA
Sbjct: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60

Query: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120
           VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS
Sbjct: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120

Query: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180
           ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR
Sbjct: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180

Query: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240
           IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW
Sbjct: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240

Query: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300
           LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK
Sbjct: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300

Query: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360
           DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL
Sbjct: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360

Query: 361 CLII 365
           CLII
Sbjct: 361 CLII 364

BLAST of Carg03654-RA vs. NCBI nr
Match: KAG6580717.1 (putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 722.2 bits (1863), Expect = 2.2e-204
Identity = 362/364 (99.45%), Postives = 362/364 (99.45%), Query Frame = 0

Query: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60
           MSFCTIGISGPELYRTSSPLA SAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA
Sbjct: 1   MSFCTIGISGPELYRTSSPLALSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60

Query: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120
           VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS
Sbjct: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120

Query: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180
           ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR
Sbjct: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180

Query: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240
           IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW
Sbjct: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240

Query: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300
           LSGEVHVRFAAVLDGERGTA ECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK
Sbjct: 241 LSGEVHVRFAAVLDGERGTATECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300

Query: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360
           DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL
Sbjct: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360

Query: 361 CLII 365
           CLII
Sbjct: 361 CLII 364

BLAST of Carg03654-RA vs. NCBI nr
Match: XP_023528246.1 (probable F-box protein At2g36090 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701.0 bits (1808), Expect = 5.2e-198
Identity = 355/366 (96.99%), Postives = 356/366 (97.27%), Query Frame = 0

Query: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60
           MSFCTIGISGPELYRTSSP A SAFSS DGDAATTSVTDIHPDVLRTHILTRLDGPALAA
Sbjct: 1   MSFCTIGISGPELYRTSSPSALSAFSSSDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60

Query: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120
           VASTSSQLHCLTAD NLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS
Sbjct: 61  VASTSSQLHCLTADPNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120

Query: 121 ETSTSSSPSSATYSRR--SRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTP 180
           ETSTSSSPSSATYSRR    RRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTP
Sbjct: 121 ETSTSSSPSSATYSRRRHRHRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTP 180

Query: 181 FRIDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQR 240
           FRIDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQR
Sbjct: 181 FRIDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQR 240

Query: 241 HWLSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLN 300
           HWLSGEVHVRFAAVL+GERGTA ECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLN
Sbjct: 241 HWLSGEVHVRFAAVLEGERGTATECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLN 300

Query: 301 GKDSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATF 360
           GKDSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATF
Sbjct: 301 GKDSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATF 360

Query: 361 LLCLII 365
           LL LII
Sbjct: 361 LLSLII 366

BLAST of Carg03654-RA vs. NCBI nr
Match: XP_022983009.1 (probable F-box protein At2g36090 [Cucurbita maxima])

HSP 1 Score: 691.4 bits (1783), Expect = 4.1e-195
Identity = 349/364 (95.88%), Postives = 352/364 (96.70%), Query Frame = 0

Query: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60
           MSFCTIGIS PELY+TSSPLA SAFSSGDGDA TTSVTDIHPDVLRTHILTRLDGPALAA
Sbjct: 1   MSFCTIGISCPELYQTSSPLALSAFSSGDGDATTTSVTDIHPDVLRTHILTRLDGPALAA 60

Query: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120
           VA TSSQLHCLT D NLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSY+S
Sbjct: 61  VACTSSQLHCLTTDPNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYNS 120

Query: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180
           ETSTSSSPSSATY   SRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR
Sbjct: 121 ETSTSSSPSSATY---SRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180

Query: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240
           IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRA+NLSSYRPVSVQRHW
Sbjct: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAINLSSYRPVSVQRHW 240

Query: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300
           LSGEVHVRFAAVLDGERGTA ECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK
Sbjct: 241 LSGEVHVRFAAVLDGERGTATECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300

Query: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360
           DSLVILERALEGKTGRRGRKKLEEFM KKRERKERTLRREGTLD VCLAFGFLGFATFLL
Sbjct: 301 DSLVILERALEGKTGRRGRKKLEEFMEKKRERKERTLRREGTLDKVCLAFGFLGFATFLL 360

Query: 361 CLII 365
           CLII
Sbjct: 361 CLII 361

BLAST of Carg03654-RA vs. NCBI nr
Match: XP_038905521.1 (probable F-box protein At2g36090 [Benincasa hispida])

HSP 1 Score: 557.4 bits (1435), Expect = 9.3e-155
Identity = 286/363 (78.79%), Postives = 314/363 (86.50%), Query Frame = 0

Query: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60
           M   T+ IS P  YRT SP   SAFSS  GD ATTS++DIHPD+LRTHILTRLDGP LAA
Sbjct: 1   MQTYTVWISSPIPYRTFSP---SAFSSAAGDGATTSISDIHPDILRTHILTRLDGPTLAA 60

Query: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120
            A TSS+ H LT++ +LWA++C STWPSTATQRL ALISEFP+GPRSFF DSFPRLSYHS
Sbjct: 61  AACTSSEFHRLTSEPSLWADICHSTWPSTATQRLDALISEFPDGPRSFFIDSFPRLSYHS 120

Query: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180
           ETSTSS+PS ATY RR RRR H  PELISAVDIYYRGEM+FSKVVETET+SGWFRC+PFR
Sbjct: 121 ETSTSSAPSPATYIRR-RRRLHLPPELISAVDIYYRGEMIFSKVVETETVSGWFRCSPFR 180

Query: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240
           IDMLDPKDV+ TPIKFPIG GN C EL EDLTLSWIMID AGRRAMN+SSYR VSVQRHW
Sbjct: 181 IDMLDPKDVIPTPIKFPIGGGNACRELGEDLTLSWIMIDAAGRRAMNMSSYRAVSVQRHW 240

Query: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300
           LSGEV V+FA VL GERGTA E V+CGIAVTCGGWEGGNLQVREVNL+VED+DGT+L+GK
Sbjct: 241 LSGEVQVQFATVLGGERGTAAEWVKCGIAVTCGGWEGGNLQVREVNLEVEDMDGTFLDGK 300

Query: 301 DSLVILERALEGKTGRRGRK-KLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFL 360
           DSLVILERA+EGK GRRG+K KLEEF+ KK ERKER +RREGTLDM+CLAFGF GFAT  
Sbjct: 301 DSLVILERAMEGKMGRRGKKIKLEEFLEKKNERKERKVRREGTLDMMCLAFGFFGFAT-- 357

Query: 361 LCL 363
           LCL
Sbjct: 361 LCL 357

BLAST of Carg03654-RA vs. ExPASy Swiss-Prot
Match: Q9SIH5 (Probable F-box protein At2g36090 OS=Arabidopsis thaliana OX=3702 GN=At2g36090 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-71
Identity = 160/348 (45.98%), Postives = 219/348 (62.93%), Query Frame = 0

Query: 22  SSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTADTNLWAEL 81
           SS+FS       T  ++ +H D++ +HILTRLDG  LA+V+  SS LH L ++  LW+++
Sbjct: 4   SSSFS--PSTTVTDLISTVHDDIIESHILTRLDGATLASVSCASSHLHHLASNEILWSKI 63

Query: 82  CRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYSRRSRRRH 141
           CRSTWPS +             G RSFFSD++  +      S    P             
Sbjct: 64  CRSTWPSCS------------GGSRSFFSDAYSMVETAGTVSDLDRP------------- 123

Query: 142 HFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDMLDPKDVVQTPIKFPIGDG 201
              PELISAVD++YRG+++FS+VV+TET + WF+ +P RID++D KD V TPIK      
Sbjct: 124 --FPELISAVDLHYRGKLIFSRVVKTETTTAWFKSSPLRIDLVDTKDTVATPIKRRQRTE 183

Query: 202 NTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLDGERGTAM 261
           +TC +L +DLTLSWI+ID  G+RA N+SS+RPVSVQR+W+SGEV  +FA V+      A+
Sbjct: 184 DTCRDLEKDLTLSWIVIDPIGKRAANISSHRPVSVQRNWISGEVEAQFATVVG-----AV 243

Query: 262 ECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGK--TGRR-- 321
           ECV     VTCG  E   + VREV+L+VE ++GT+LNG+DSLVIL   +EGK   G R  
Sbjct: 244 ECVI--TVVTCGEEE---MHVREVSLKVEKMEGTHLNGRDSLVILRSVMEGKRVNGSRRE 303

Query: 322 --GRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL-CL 363
              +K+ EEFM KKRE KE+ +R E   D++ +AFG LGF   ++ CL
Sbjct: 304 VESKKRHEEFMEKKREMKEKKMRVESVFDILTVAFGILGFVLLVVFCL 312

BLAST of Carg03654-RA vs. ExPASy Swiss-Prot
Match: Q9XIN8 (F-box protein At2g27310 OS=Arabidopsis thaliana OX=3702 GN=At2g27310 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.2e-65
Identity = 143/337 (42.43%), Postives = 203/337 (60.24%), Query Frame = 0

Query: 36  SVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTADTNLWAELCRSTWPSTATQRLH 95
           S++ +H D+++T ILTRLDGP LA+ A+TSS L  L  +  LW EL   TWPS    R+ 
Sbjct: 11  SISTLHSDIIQTQILTRLDGPTLASTATTSSYLQTLCTEEKLWQELSIDTWPSINDPRVV 70

Query: 96  ALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYY 155
             IS FP+G RSFF+DS+P  + H+  S    P +                LISAVD+YY
Sbjct: 71  QAISSFPSGYRSFFADSYP-FTEHTWQSEKHDPPTG---------------LISAVDLYY 130

Query: 156 RGEMVFSKVVETET---LSGWFRCTPFRIDMLDPKDVVQTPIKFPIGDGNTCW--ELTED 215
           RGE+++SKV E ET    SGWF  +PFR+D+LDPK+ VQT I++P GD    W  ++ E 
Sbjct: 131 RGEIIYSKVQEMETEKGKSGWFLSSPFRVDILDPKESVQTRIRYPGGD-YEAWVKDMEES 190

Query: 216 LTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLDGERGTAMECVQCGIAV 275
           + L+WI+ID   +RA N+SS + VS +R+WL+G++ +RF+ V+  E       V CG A 
Sbjct: 191 MKLNWILIDPIKKRAANISSRKAVSARRNWLTGDLEIRFSTVVTAEAAEVAAVVSCGSA- 250

Query: 276 TCGGWE------GGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGKTG------RRG 335
               W+      GG + VR+V LQVED++G  + G+DSLVIL+  L+GK        RR 
Sbjct: 251 --EAWKEVDEEVGGEIHVRDVRLQVEDIEGKCMKGRDSLVILQGLLDGKRSCKDDEERRA 310

Query: 336 RKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGF 356
           +++ EE++  K + +E   RRE   D +C+ FGF  F
Sbjct: 311 KERYEEYVRMKIQWRENKERREKAQDTICMIFGFSMF 327

BLAST of Carg03654-RA vs. ExPASy Swiss-Prot
Match: Q2V3R1 (F-box protein At3g44326 OS=Arabidopsis thaliana OX=3702 GN=At3g44326 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.5e-54
Identity = 136/359 (37.88%), Postives = 206/359 (57.38%), Query Frame = 0

Query: 21  ASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTADTNLWAE 80
           +SS+         +  +  +  DVLR++ILTRLDG +LAA++ T S L+   +D +LW +
Sbjct: 5   SSSSTVEQPSRGGSPGINAVSSDVLRSNILTRLDGSSLAALSCTCSNLNSFCSDESLWRQ 64

Query: 81  LCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYSRRSRRR 140
            C +TWPST+  R+ ++IS FP+G R+FFSDSFP L +  +   +  PS           
Sbjct: 65  QCSATWPSTSDTRVQSIISTFPDGHRTFFSDSFPFLEH--DVGINLPPSVD--------- 124

Query: 141 HHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDMLDPKDVVQTPIKFPIGD 200
              T ELISAVDI+Y+ +++FS+V  TET+SGWF C+P R+D+++PK+++ T +      
Sbjct: 125 ---TSELISAVDIFYKDDVIFSRVHVTETVSGWFLCSPMRVDLVEPKELIPTRVLVTDQC 184

Query: 201 GNTCW--ELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLDGERG 260
            +  W  +L E+L+LSWI+ID   +RA ++S+ +PVSV RHWL+GEVHV+F+++      
Sbjct: 185 KDDTWKSDLEENLSLSWILIDPTCKRAADVSTRKPVSVHRHWLTGEVHVKFSSIFVVGNK 244

Query: 261 TAMECVQCGIAVTCGGW-----EGGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGK 320
              E V+  + V    +     E   +Q+REV+L  ED DG  L GK SL IL  A+  K
Sbjct: 245 KRSEQVEFTVTVVLAVFNRREEETAVMQIREVSLVAEDKDGRNLGGKVSLEILVAAMGMK 304

Query: 321 TGRR------GRKKLEEFMAKKRERKERTLRR--EGTLDMVCLAFGFLGFATFLLCLII 365
              R      G++K  E+M +K  + E   RR  E  ++        L    FLLC  +
Sbjct: 305 RRFRAGGEEEGKEKYIEYMERKTAKAEMKWRRGKETAMETAACWIAVL-LLGFLLCFYL 348

BLAST of Carg03654-RA vs. ExPASy TrEMBL
Match: A0A6J1FAG7 (probable F-box protein At2g36090 OS=Cucurbita moschata OX=3662 GN=LOC111442296 PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.3e-206
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60
           MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA
Sbjct: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60

Query: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120
           VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS
Sbjct: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120

Query: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180
           ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR
Sbjct: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180

Query: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240
           IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW
Sbjct: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240

Query: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300
           LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK
Sbjct: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300

Query: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360
           DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL
Sbjct: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360

Query: 361 CLII 365
           CLII
Sbjct: 361 CLII 364

BLAST of Carg03654-RA vs. ExPASy TrEMBL
Match: A0A6J1J640 (probable F-box protein At2g36090 OS=Cucurbita maxima OX=3661 GN=LOC111481680 PE=4 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.0e-195
Identity = 349/364 (95.88%), Postives = 352/364 (96.70%), Query Frame = 0

Query: 1   MSFCTIGISGPELYRTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAA 60
           MSFCTIGIS PELY+TSSPLA SAFSSGDGDA TTSVTDIHPDVLRTHILTRLDGPALAA
Sbjct: 1   MSFCTIGISCPELYQTSSPLALSAFSSGDGDATTTSVTDIHPDVLRTHILTRLDGPALAA 60

Query: 61  VASTSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHS 120
           VA TSSQLHCLT D NLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSY+S
Sbjct: 61  VACTSSQLHCLTTDPNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYNS 120

Query: 121 ETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180
           ETSTSSSPSSATY   SRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR
Sbjct: 121 ETSTSSSPSSATY---SRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFR 180

Query: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHW 240
           IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRA+NLSSYRPVSVQRHW
Sbjct: 181 IDMLDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAINLSSYRPVSVQRHW 240

Query: 241 LSGEVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300
           LSGEVHVRFAAVLDGERGTA ECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK
Sbjct: 241 LSGEVHVRFAAVLDGERGTATECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGK 300

Query: 301 DSLVILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL 360
           DSLVILERALEGKTGRRGRKKLEEFM KKRERKERTLRREGTLD VCLAFGFLGFATFLL
Sbjct: 301 DSLVILERALEGKTGRRGRKKLEEFMEKKRERKERTLRREGTLDKVCLAFGFLGFATFLL 360

Query: 361 CLII 365
           CLII
Sbjct: 361 CLII 361

BLAST of Carg03654-RA vs. ExPASy TrEMBL
Match: A0A0A0LAY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G777680 PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.0e-146
Identity = 267/349 (76.50%), Postives = 296/349 (84.81%), Query Frame = 0

Query: 15  RTSSPLASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTAD 74
           +T SPL   AF S D DAA+TS++DIHPD+LRTHILTRLDGPALA+ A TSS+LH LTA+
Sbjct: 4   QTFSPL---AFPSADVDAASTSISDIHPDILRTHILTRLDGPALASAACTSSELHRLTAE 63

Query: 75  TNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYS 134
             LW+ +C STWPST T RLHALISEFP+GPRSFFSDS PR S HSETSTSS  S ATY 
Sbjct: 64  PKLWSAICHSTWPSTTTPRLHALISEFPDGPRSFFSDSLPRGSNHSETSTSSPTSPATYC 123

Query: 135 RRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDMLDPKDVVQTPI 194
                R H  PELISAVDIYYRGEM+FSKVVETET+SGWFRC+PFRIDMLDPKDVV TPI
Sbjct: 124 ----HRRHLPPELISAVDIYYRGEMIFSKVVETETVSGWFRCSPFRIDMLDPKDVVPTPI 183

Query: 195 KFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLD 254
           KFPIG+GN C EL EDLTLSWI+ID AGRRAMNLSSYR V V++HWLSGEVHV F  VL 
Sbjct: 184 KFPIGEGNACRELGEDLTLSWIVIDGAGRRAMNLSSYRAVKVEQHWLSGEVHVEFGTVLG 243

Query: 255 GERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGKT 314
           GE+GTA E V+CGIAVTCGGWEGGNLQVREVNLQVED+DGT+L+GKDSLVILERA+EGK 
Sbjct: 244 GEKGTAAEWVKCGIAVTCGGWEGGNLQVREVNLQVEDMDGTFLDGKDSLVILERAMEGKK 303

Query: 315 GRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLLCLI 364
           GR+GRK+L EFM KKRERKE  +RREGTLDM+CLAFGF GFAT  LC +
Sbjct: 304 GRKGRKRLNEFMEKKRERKEMKVRREGTLDMMCLAFGFFGFATLCLCFL 345

BLAST of Carg03654-RA vs. ExPASy TrEMBL
Match: A0A5A7TK58 (Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G001880 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 7.0e-140
Identity = 264/360 (73.33%), Postives = 297/360 (82.50%), Query Frame = 0

Query: 5   TIGISGPELYRTSSPLASSAFS-SGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVAS 64
           T+GIS       S+P+ +  FS S   D A+TS++DIHPD+LRTHIL+RLDGP LAA A 
Sbjct: 5   TVGIS-------SAPMPNHTFSPSAVVDGASTSISDIHPDILRTHILSRLDGPDLAAAAC 64

Query: 65  TSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETS 124
           TSS+LH LTA+  LWA +C STWPST T RL ALISEFP+GPRSFF DSFPR S HSETS
Sbjct: 65  TSSELHRLTAEPKLWAAICHSTWPSTTTPRLDALISEFPDGPRSFFYDSFPRGSNHSETS 124

Query: 125 TSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDM 184
           T SSPS ATY     RR H   ELISAVDIYYRGEM+FS+VVETET+SGWFRC+PFRIDM
Sbjct: 125 T-SSPSPATYC----RRRHLPMELISAVDIYYRGEMIFSRVVETETVSGWFRCSPFRIDM 184

Query: 185 LDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSG 244
           LDPKDVV T IKFPIGDGNTC EL EDLTLSWI+ID AGRRA+NLSSYR V V+RHWLSG
Sbjct: 185 LDPKDVVPTTIKFPIGDGNTCRELAEDLTLSWIVIDGAGRRAVNLSSYRAVKVERHWLSG 244

Query: 245 EVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGKDSL 304
           EVHV F  VL GERGTA E V+CGIAVTCGGWEGGNLQVREVNLQVED+DGT+L+GK+SL
Sbjct: 245 EVHVEFGTVLGGERGTAAEWVKCGIAVTCGGWEGGNLQVREVNLQVEDMDGTFLDGKESL 304

Query: 305 VILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLLCLI 364
           VILERA+E   GR+GRK+L+EFM KK+ERKE  +RREGTLDM+CLAFGF GFAT  LC +
Sbjct: 305 VILERAME---GRKGRKRLDEFMEKKKERKEMKVRREGTLDMMCLAFGFFGFATLCLCFL 349

BLAST of Carg03654-RA vs. ExPASy TrEMBL
Match: A0A1S3B692 (probable F-box protein At1g60180 OS=Cucumis melo OX=3656 GN=LOC103486639 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 7.0e-140
Identity = 264/360 (73.33%), Postives = 297/360 (82.50%), Query Frame = 0

Query: 5   TIGISGPELYRTSSPLASSAFS-SGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVAS 64
           T+GIS       S+P+ +  FS S   D A+TS++DIHPD+LRTHIL+RLDGP LAA A 
Sbjct: 5   TVGIS-------SAPMPNHTFSPSAVVDGASTSISDIHPDILRTHILSRLDGPDLAAAAC 64

Query: 65  TSSQLHCLTADTNLWAELCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETS 124
           TSS+LH LTA+  LWA +C STWPST T RL ALISEFP+GPRSFF DSFPR S HSETS
Sbjct: 65  TSSELHRLTAEPKLWAAICHSTWPSTTTPRLDALISEFPDGPRSFFYDSFPRGSNHSETS 124

Query: 125 TSSSPSSATYSRRSRRRHHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDM 184
           T SSPS ATY     RR H   ELISAVDIYYRGEM+FS+VVETET+SGWFRC+PFRIDM
Sbjct: 125 T-SSPSPATYC----RRRHLPMELISAVDIYYRGEMIFSRVVETETVSGWFRCSPFRIDM 184

Query: 185 LDPKDVVQTPIKFPIGDGNTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSG 244
           LDPKDVV T IKFPIGDGNTC EL EDLTLSWI+ID AGRRA+NLSSYR V V+RHWLSG
Sbjct: 185 LDPKDVVPTTIKFPIGDGNTCRELAEDLTLSWIVIDGAGRRAVNLSSYRAVKVERHWLSG 244

Query: 245 EVHVRFAAVLDGERGTAMECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGKDSL 304
           EVHV F  VL GERGTA E V+CGIAVTCGGWEGGNLQVREVNLQVED+DGT+L+GK+SL
Sbjct: 245 EVHVEFGTVLGGERGTAAEWVKCGIAVTCGGWEGGNLQVREVNLQVEDMDGTFLDGKESL 304

Query: 305 VILERALEGKTGRRGRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLLCLI 364
           VILERA+E   GR+GRK+L+EFM KK+ERKE  +RREGTLDM+CLAFGF GFAT  LC +
Sbjct: 305 VILERAME---GRKGRKRLDEFMEKKKERKEMKVRREGTLDMMCLAFGFFGFATLCLCFL 349

BLAST of Carg03654-RA vs. TAIR 10
Match: AT2G36090.1 (F-box family protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-72
Identity = 160/348 (45.98%), Postives = 219/348 (62.93%), Query Frame = 0

Query: 22  SSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTADTNLWAEL 81
           SS+FS       T  ++ +H D++ +HILTRLDG  LA+V+  SS LH L ++  LW+++
Sbjct: 4   SSSFS--PSTTVTDLISTVHDDIIESHILTRLDGATLASVSCASSHLHHLASNEILWSKI 63

Query: 82  CRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYSRRSRRRH 141
           CRSTWPS +             G RSFFSD++  +      S    P             
Sbjct: 64  CRSTWPSCS------------GGSRSFFSDAYSMVETAGTVSDLDRP------------- 123

Query: 142 HFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDMLDPKDVVQTPIKFPIGDG 201
              PELISAVD++YRG+++FS+VV+TET + WF+ +P RID++D KD V TPIK      
Sbjct: 124 --FPELISAVDLHYRGKLIFSRVVKTETTTAWFKSSPLRIDLVDTKDTVATPIKRRQRTE 183

Query: 202 NTCWELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLDGERGTAM 261
           +TC +L +DLTLSWI+ID  G+RA N+SS+RPVSVQR+W+SGEV  +FA V+      A+
Sbjct: 184 DTCRDLEKDLTLSWIVIDPIGKRAANISSHRPVSVQRNWISGEVEAQFATVVG-----AV 243

Query: 262 ECVQCGIAVTCGGWEGGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGK--TGRR-- 321
           ECV     VTCG  E   + VREV+L+VE ++GT+LNG+DSLVIL   +EGK   G R  
Sbjct: 244 ECVI--TVVTCGEEE---MHVREVSLKVEKMEGTHLNGRDSLVILRSVMEGKRVNGSRRE 303

Query: 322 --GRKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGFATFLL-CL 363
              +K+ EEFM KKRE KE+ +R E   D++ +AFG LGF   ++ CL
Sbjct: 304 VESKKRHEEFMEKKREMKEKKMRVESVFDILTVAFGILGFVLLVVFCL 312

BLAST of Carg03654-RA vs. TAIR 10
Match: AT2G27310.1 (F-box family protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.0e-66
Identity = 143/337 (42.43%), Postives = 203/337 (60.24%), Query Frame = 0

Query: 36  SVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTADTNLWAELCRSTWPSTATQRLH 95
           S++ +H D+++T ILTRLDGP LA+ A+TSS L  L  +  LW EL   TWPS    R+ 
Sbjct: 11  SISTLHSDIIQTQILTRLDGPTLASTATTSSYLQTLCTEEKLWQELSIDTWPSINDPRVV 70

Query: 96  ALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYSRRSRRRHHFTPELISAVDIYY 155
             IS FP+G RSFF+DS+P  + H+  S    P +                LISAVD+YY
Sbjct: 71  QAISSFPSGYRSFFADSYP-FTEHTWQSEKHDPPTG---------------LISAVDLYY 130

Query: 156 RGEMVFSKVVETET---LSGWFRCTPFRIDMLDPKDVVQTPIKFPIGDGNTCW--ELTED 215
           RGE+++SKV E ET    SGWF  +PFR+D+LDPK+ VQT I++P GD    W  ++ E 
Sbjct: 131 RGEIIYSKVQEMETEKGKSGWFLSSPFRVDILDPKESVQTRIRYPGGD-YEAWVKDMEES 190

Query: 216 LTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLDGERGTAMECVQCGIAV 275
           + L+WI+ID   +RA N+SS + VS +R+WL+G++ +RF+ V+  E       V CG A 
Sbjct: 191 MKLNWILIDPIKKRAANISSRKAVSARRNWLTGDLEIRFSTVVTAEAAEVAAVVSCGSA- 250

Query: 276 TCGGWE------GGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGKTG------RRG 335
               W+      GG + VR+V LQVED++G  + G+DSLVIL+  L+GK        RR 
Sbjct: 251 --EAWKEVDEEVGGEIHVRDVRLQVEDIEGKCMKGRDSLVILQGLLDGKRSCKDDEERRA 310

Query: 336 RKKLEEFMAKKRERKERTLRREGTLDMVCLAFGFLGF 356
           +++ EE++  K + +E   RRE   D +C+ FGF  F
Sbjct: 311 KERYEEYVRMKIQWRENKERREKAQDTICMIFGFSMF 327

BLAST of Carg03654-RA vs. TAIR 10
Match: AT3G44326.1 (F-box family protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.1e-55
Identity = 136/359 (37.88%), Postives = 206/359 (57.38%), Query Frame = 0

Query: 21  ASSAFSSGDGDAATTSVTDIHPDVLRTHILTRLDGPALAAVASTSSQLHCLTADTNLWAE 80
           +SS+         +  +  +  DVLR++ILTRLDG +LAA++ T S L+   +D +LW +
Sbjct: 5   SSSSTVEQPSRGGSPGINAVSSDVLRSNILTRLDGSSLAALSCTCSNLNSFCSDESLWRQ 64

Query: 81  LCRSTWPSTATQRLHALISEFPNGPRSFFSDSFPRLSYHSETSTSSSPSSATYSRRSRRR 140
            C +TWPST+  R+ ++IS FP+G R+FFSDSFP L +  +   +  PS           
Sbjct: 65  QCSATWPSTSDTRVQSIISTFPDGHRTFFSDSFPFLEH--DVGINLPPSVD--------- 124

Query: 141 HHFTPELISAVDIYYRGEMVFSKVVETETLSGWFRCTPFRIDMLDPKDVVQTPIKFPIGD 200
              T ELISAVDI+Y+ +++FS+V  TET+SGWF C+P R+D+++PK+++ T +      
Sbjct: 125 ---TSELISAVDIFYKDDVIFSRVHVTETVSGWFLCSPMRVDLVEPKELIPTRVLVTDQC 184

Query: 201 GNTCW--ELTEDLTLSWIMIDTAGRRAMNLSSYRPVSVQRHWLSGEVHVRFAAVLDGERG 260
            +  W  +L E+L+LSWI+ID   +RA ++S+ +PVSV RHWL+GEVHV+F+++      
Sbjct: 185 KDDTWKSDLEENLSLSWILIDPTCKRAADVSTRKPVSVHRHWLTGEVHVKFSSIFVVGNK 244

Query: 261 TAMECVQCGIAVTCGGW-----EGGNLQVREVNLQVEDLDGTYLNGKDSLVILERALEGK 320
              E V+  + V    +     E   +Q+REV+L  ED DG  L GK SL IL  A+  K
Sbjct: 245 KRSEQVEFTVTVVLAVFNRREEETAVMQIREVSLVAEDKDGRNLGGKVSLEILVAAMGMK 304

Query: 321 TGRR------GRKKLEEFMAKKRERKERTLRR--EGTLDMVCLAFGFLGFATFLLCLII 365
              R      G++K  E+M +K  + E   RR  E  ++        L    FLLC  +
Sbjct: 305 RRFRAGGEEEGKEKYIEYMERKTAKAEMKWRRGKETAMETAACWIAVL-LLGFLLCFYL 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022935408.18.8e-206100.00probable F-box protein At2g36090 [Cucurbita moschata] >KAG7017474.1 putative F-b... [more]
KAG6580717.12.2e-20499.45putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023528246.15.2e-19896.99probable F-box protein At2g36090 [Cucurbita pepo subsp. pepo][more]
XP_022983009.14.1e-19595.88probable F-box protein At2g36090 [Cucurbita maxima][more]
XP_038905521.19.3e-15578.79probable F-box protein At2g36090 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SIH51.7e-7145.98Probable F-box protein At2g36090 OS=Arabidopsis thaliana OX=3702 GN=At2g36090 PE... [more]
Q9XIN84.2e-6542.43F-box protein At2g27310 OS=Arabidopsis thaliana OX=3702 GN=At2g27310 PE=2 SV=1[more]
Q2V3R11.5e-5437.88F-box protein At3g44326 OS=Arabidopsis thaliana OX=3702 GN=At3g44326 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FAG74.3e-206100.00probable F-box protein At2g36090 OS=Cucurbita moschata OX=3662 GN=LOC111442296 P... [more]
A0A6J1J6402.0e-19595.88probable F-box protein At2g36090 OS=Cucurbita maxima OX=3661 GN=LOC111481680 PE=... [more]
A0A0A0LAY81.0e-14676.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G777680 PE=4 SV=1[more]
A0A5A7TK587.0e-14073.33Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2... [more]
A0A1S3B6927.0e-14073.33probable F-box protein At1g60180 OS=Cucumis melo OX=3656 GN=LOC103486639 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT2G36090.11.2e-7245.98F-box family protein [more]
AT2G27310.13.0e-6642.43F-box family protein [more]
AT3G44326.11.1e-5537.88F-box family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1280.50coord: 31..144
e-value: 7.5E-6
score: 27.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..133
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..138
NoneNo IPR availablePANTHERPTHR33736:SF13OS12G0156500 PROTEINcoord: 29..358
NoneNo IPR availablePANTHERPTHR33736F-BOX PROTEIN-RELATEDcoord: 29..358
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 42..107

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg03654Carg03654gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03654-RA:1Carg03654-RA:1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03654-RA:cdsCarg03654-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg03654-RACarg03654-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding