Carg03155-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03155-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding EF-hand family protein
Location	Carg_Chr20: 2828114 .. 2828479 (+)
Sequence length	366
RNA-Seq Expression	Carg03155-RA
Synteny	Carg03155-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGAAAGGGTGGTGGTGGTGGTGGTGGTGGTGGTTTCCACGATCTGCTGCCGGTAATGGCGGAGAAATTGGGAGGAGAAGGGCTGATAAACGAGCTCTGTAATGGGTTTCAGATGCTGATGGACAAGGAGAAGGGCGTGATCACTTTAGAGAGCCTTAGGAGAAACTCTTCCCTTTTGGGTTTGTGGGATATGGGAGAAGAGGAGCTTGTGAGCATGATGAGGGAAGGGGACTTGGATGGCGATGGCGCTTTGAATCAAATGGAGTTTTGCGTTTTGATGTTCAGATTAAGCCCTGAATTGATGGAAGAGTCTCTGTATTGGCTTGATGACGCTTTGGAAAAGGAGTTTAAAGCATCTTGA

mRNA sequence

ATGGCGAGAAAGGGTGGTGGTGGTGGTGGTGGTGGTGGTTTCCACGATCTGCTGCCGGTAATGGCGGAGAAATTGGGAGGAGAAGGGCTGATAAACGAGCTCTGTAATGGGTTTCAGATGCTGATGGACAAGGAGAAGGGCGTGATCACTTTAGAGAGCCTTAGGAGAAACTCTTCCCTTTTGGGTTTGTGGGATATGGGAGAAGAGGAGCTTGTGAGCATGATGAGGGAAGGGGACTTGGATGGCGATGGCGCTTTGAATCAAATGGAGTTTTGCGTTTTGATGTTCAGATTAAGCCCTGAATTGATGGAAGAGTCTCTGTATTGGCTTGATGACGCTTTGGAAAAGGAGTTTAAAGCATCTTGA

Coding sequence (CDS)

ATGGCGAGAAAGGGTGGTGGTGGTGGTGGTGGTGGTGGTTTCCACGATCTGCTGCCGGTAATGGCGGAGAAATTGGGAGGAGAAGGGCTGATAAACGAGCTCTGTAATGGGTTTCAGATGCTGATGGACAAGGAGAAGGGCGTGATCACTTTAGAGAGCCTTAGGAGAAACTCTTCCCTTTTGGGTTTGTGGGATATGGGAGAAGAGGAGCTTGTGAGCATGATGAGGGAAGGGGACTTGGATGGCGATGGCGCTTTGAATCAAATGGAGTTTTGCGTTTTGATGTTCAGATTAAGCCCTGAATTGATGGAAGAGTCTCTGTATTGGCTTGATGACGCTTTGGAAAAGGAGTTTAAAGCATCTTGA

Protein sequence

MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKAS

Homology

[Close]

BLAST of Carg03155-RA vs. NCBI nr
Match: XP_022944617.1 (calcium-binding protein PBP1-like [Cucurbita moschata] >KAG7010710.1 Calcium-binding protein PBP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 242.7 bits (618), Expect = 1.7e-60
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60
           MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL
Sbjct: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60

Query: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 120
           LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA
Sbjct: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of Carg03155-RA vs. NCBI nr
Match: XP_022986403.1 (calcium-binding protein PBP1-like [Cucurbita maxima])

HSP 1 Score: 238.0 bits (606), Expect = 4.1e-59
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MARK-GGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS 60
           MARK GGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS
Sbjct: 1   MARKGGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS 60

Query: 61  LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFK 120
           LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFK
Sbjct: 61  LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFK 120

Query: 121 AS 122
           AS
Sbjct: 121 AS 122

[Close]

BLAST of Carg03155-RA vs. NCBI nr
Match: KAG6570861.1 (Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 233.0 bits (593), Expect = 1.3e-57
Identity = 118/121 (97.52%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60
           MARK   GGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL
Sbjct: 1   MARK---GGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60

Query: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 120
           LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA
Sbjct: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 118

Query: 121 S 122
           S
Sbjct: 121 S 118

[Close]

BLAST of Carg03155-RA vs. NCBI nr
Match: XP_023512862.1 (calcium-binding protein PBP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 230.7 bits (587), Expect = 6.6e-57
Identity = 117/121 (96.69%), Postives = 117/121 (96.69%), Query Frame = 0


Query: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60
           MARK   GGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLR NSSL
Sbjct: 1   MARK---GGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRTNSSL 60

Query: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 120
           LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA
Sbjct: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 118

Query: 121 S 122
           S
Sbjct: 121 S 118

[Close]

BLAST of Carg03155-RA vs. NCBI nr
Match: XP_004140055.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN46674.1 hypothetical protein Csa_020739 [Cucumis sativus])

HSP 1 Score: 184.5 bits (467), Expect = 5.4e-43
Identity = 92/109 (84.40%), Postives = 98/109 (89.91%), Query Frame = 0


Query: 10  GGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEE 69
           GG  F DLLPVMAEKLGGEGLI ELCNGF++LMDKEKGVITLESLRRNS LLG+ DM EE
Sbjct: 5   GGANFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGVITLESLRRNSWLLGVRDMAEE 64

Query: 70  ELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119
           EL+SMMREGDLDGDGALNQMEFCVLMFRLSP+LM+ SLYWL  ALE EF
Sbjct: 65  ELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLYWLQLALENEF 113

[Close]

BLAST of Carg03155-RA vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 8.2e-34
Identity = 70/106 (66.04%), Postives = 87/106 (82.08%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRN-SSLLGLWDMGEEELV 73
           FHD LP MA  LGGEGLI ELCNGF++LMD+EKGVIT ESLRRN +++LGL D+ +E++ 
Sbjct: 19  FHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDVR 78

Query: 74  SMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119
            M++EGD D DGALNQMEFCVLMFRLSP+LME S   + + +E+EF
Sbjct: 79  CMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of Carg03155-RA vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 4.5e-32
Identity = 68/106 (64.15%), Postives = 84/106 (79.25%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRN-SSLLGLWDMGEEELV 73
           F D  P MA KLGGEGLI E+C GF++LMDK+KGVIT ESLRRN S++LGL D+ ++++ 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 74  SMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119
            M+ EGD D DGALNQMEFCVLMFRLSPELME S   + + +E+EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of Carg03155-RA vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 7.2e-30
Identity = 62/104 (59.62%), Postives = 80/104 (76.92%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEELVS 73
           + D+LPVMAEK+  E  ++ELC GF +L D E+ +IT ESLRRNS +LG+  M +E+   
Sbjct: 22  YEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKEDAQG 81

Query: 74  MMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKE 118
           M+REGDLDGDGALNQ EFCVLM RLSPE+ME++  WL+ AL +E
Sbjct: 82  MVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of Carg03155-RA vs. ExPASy Swiss-Prot
Match: P41209 (Centrin-1 OS=Mus musculus OX=10090 GN=Cetn1 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-07
Identity = 30/82 (36.59%), Postives = 49/82 (59.76%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEELVS 73
           F+D L VM +K+  +    E+   F++  D E G I+ ++L+R ++ LG   + +EEL  
Sbjct: 86  FNDFLAVMTQKMAEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELG-ESLTDEELQE 145

Query: 74  MMREGDLDGDGALNQMEFCVLM 96
           M+ E D DGDG +N+ EF  +M
Sbjct: 146 MIDEADRDGDGEVNEEEFLKIM 166

[Close]

BLAST of Carg03155-RA vs. ExPASy Swiss-Prot
Match: Q2TBN3 (Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.9e-07
Identity = 33/92 (35.87%), Postives = 52/92 (56.52%), Query Frame = 0


Query: 8   GGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMG 67
           G G   F D L VM +K+  +    E+   F++  D E G I+ ++L+R +  LG  ++ 
Sbjct: 80  GTGKMNFSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLS 139

Query: 68  EEELVSMMREGDLDGDGALNQMEFCVLMFRLS 100
           +EEL  M+ E D DGDG +N+ EF  +M + S
Sbjct: 140 DEELQEMIDEADRDGDGEVNEQEFLRIMKKTS 170

[Close]

BLAST of Carg03155-RA vs. ExPASy TrEMBL
Match: A0A6J1FW37 (calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111449023 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 8.1e-61
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60
           MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL
Sbjct: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60

Query: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 120
           LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA
Sbjct: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of Carg03155-RA vs. ExPASy TrEMBL
Match: A0A6J1JGE3 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111484150 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.0e-59
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MARK-GGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS 60
           MARK GGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS
Sbjct: 1   MARKGGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS 60

Query: 61  LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFK 120
           LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFK
Sbjct: 61  LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFK 120

Query: 121 AS 122
           AS
Sbjct: 121 AS 122

[Close]

BLAST of Carg03155-RA vs. ExPASy TrEMBL
Match: A0A0A0KCV2 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G120930 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.6e-43
Identity = 92/109 (84.40%), Postives = 98/109 (89.91%), Query Frame = 0


Query: 10  GGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEE 69
           GG  F DLLPVMAEKLGGEGLI ELCNGF++LMDKEKGVITLESLRRNS LLG+ DM EE
Sbjct: 5   GGANFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGVITLESLRRNSWLLGVRDMAEE 64

Query: 70  ELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119
           EL+SMMREGDLDGDGALNQMEFCVLMFRLSP+LM+ SLYWL  ALE EF
Sbjct: 65  ELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLYWLQLALENEF 113

[Close]

BLAST of Carg03155-RA vs. ExPASy TrEMBL
Match: B9I6J5 (EF-hand domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_013G151000 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.1e-41
Identity = 83/107 (77.57%), Postives = 98/107 (91.59%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEELVS 73
           F DLLPVMA KLGGEGLINELCNGFQ+LMDK++GVIT+ESLRRNSS LGL D+ ++EL S
Sbjct: 10  FEDLLPVMANKLGGEGLINELCNGFQLLMDKDRGVITMESLRRNSSFLGLQDLSDDELAS 69

Query: 74  MMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 121
           M++EGDLDGDGALNQMEFCVLMFRLSPELM+ES +WL++A E+E K+
Sbjct: 70  MVKEGDLDGDGALNQMEFCVLMFRLSPELMQESRFWLEEAFEEELKS 116

[Close]

BLAST of Carg03155-RA vs. ExPASy TrEMBL
Match: A0A6M2ERA4 (EF-hand domain-containing protein OS=Populus davidiana OX=266767 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.1e-41
Identity = 83/107 (77.57%), Postives = 98/107 (91.59%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEELVS 73
           F DLLPV+A KLGGEGLINELCNGFQ+LMDK++GVIT+ESLRRNSS LGL D+ +EEL S
Sbjct: 11  FEDLLPVLANKLGGEGLINELCNGFQLLMDKDRGVITMESLRRNSSFLGLQDLSDEELAS 70

Query: 74  MMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEFKA 121
           M++EGDLDGDGALNQMEFCVLMFRLSPELM+ES +WL++A E+E K+
Sbjct: 71  MVKEGDLDGDGALNQMEFCVLMFRLSPELMQESRFWLEEAFEEELKS 117

[Close]

BLAST of Carg03155-RA vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 144.4 bits (363), Expect = 5.8e-35
Identity = 70/106 (66.04%), Postives = 87/106 (82.08%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRN-SSLLGLWDMGEEELV 73
           FHD LP MA  LGGEGLI ELCNGF++LMD+EKGVIT ESLRRN +++LGL D+ +E++ 
Sbjct: 19  FHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDVR 78

Query: 74  SMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119
            M++EGD D DGALNQMEFCVLMFRLSP+LME S   + + +E+EF
Sbjct: 79  CMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of Carg03155-RA vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 138.7 bits (348), Expect = 3.2e-33
Identity = 68/106 (64.15%), Postives = 84/106 (79.25%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRN-SSLLGLWDMGEEELV 73
           F D  P MA KLGGEGLI E+C GF++LMDK+KGVIT ESLRRN S++LGL D+ ++++ 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 74  SMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119
            M+ EGD D DGALNQMEFCVLMFRLSPELME S   + + +E+EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of Carg03155-RA vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 131.3 bits (329), Expect = 5.1e-31
Identity = 62/104 (59.62%), Postives = 80/104 (76.92%), Query Frame = 0


Query: 14  FHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEELVS 73
           + D+LPVMAEK+  E  ++ELC GF +L D E+ +IT ESLRRNS +LG+  M +E+   
Sbjct: 22  YEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKEDAQG 81

Query: 74  MMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKE 118
           M+REGDLDGDGALNQ EFCVLM RLSPE+ME++  WL+ AL +E
Sbjct: 82  MVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of Carg03155-RA vs. TAIR 10
Match: AT4G23650.1 (calcium-dependent protein kinase 6 )

HSP 1 Score: 48.1 bits (113), Expect = 5.7e-06
Identity = 31/91 (34.07%), Postives = 47/91 (51.65%), Query Frame = 0


Query: 18  LPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMGEEELVSMMRE 77
           L V+AE L  E +I  L   F+ L     G++TLE LR     LG   + E E+  +M  
Sbjct: 369 LKVIAENLSEEEIIG-LKEMFKSLDTDNNGIVTLEELRTGLPKLGS-KISEAEIRQLMEA 428

Query: 78  GDLDGDGALNQMEFCVLMFRLSPELMEESLY 109
            D+DGDG+++ +EF      ++    E+ LY
Sbjct: 429 ADMDGDGSIDYLEFISATMHMNRIEREDHLY 457

[Close]

BLAST of Carg03155-RA vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 45.1 bits (105), Expect = 4.8e-05
Identity = 30/88 (34.09%), Postives = 46/88 (52.27%), Query Frame = 0


Query: 8   GGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGLWDMG 67
           G G   F + L +MA+K+       EL   F++    + G I+   LR   + LG   + 
Sbjct: 60  GNGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG-EKLT 119

Query: 68  EEELVSMMREGDLDGDGALNQMEFCVLM 96
           +EE+  M+RE D+DGDG +N  EF  +M
Sbjct: 120 DEEVEEMIREADVDGDGQINYEEFVKIM 146

The following BLAST results are available for this feature:

BLAST of Carg03155-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944617.1	1.7e-60	100.00	calcium-binding protein PBP1-like [Cucurbita moschata] >KAG7010710.1 Calcium-bin...	[more]
XP_022986403.1	4.1e-59	99.18	calcium-binding protein PBP1-like [Cucurbita maxima]	[more]
KAG6570861.1	1.3e-57	97.52	Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023512862.1	6.6e-57	96.69	calcium-binding protein PBP1-like [Cucurbita pepo subsp. pepo]	[more]
XP_004140055.1	5.4e-43	84.40	calcium-binding protein KRP1 [Cucumis sativus] >KGN46674.1 hypothetical protein ...	[more]

BLAST of Carg03155-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81831	8.2e-34	66.04	Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1	[more]
Q9LSQ6	4.5e-32	64.15	Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1	[more]
Q9ZPX9	7.2e-30	59.62	Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2	[more]
P41209	1.3e-07	36.59	Centrin-1 OS=Mus musculus OX=10090 GN=Cetn1 PE=1 SV=1	[more]
Q2TBN3	2.9e-07	35.87	Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1	[more]

BLAST of Carg03155-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FW37	8.1e-61	100.00	calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111449023 ...	[more]
A0A6J1JGE3	2.0e-59	99.18	calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111484150 PE...	[more]
A0A0A0KCV2	2.6e-43	84.40	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G120930 PE=...	[more]
B9I6J5	1.1e-41	77.57	EF-hand domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_013G15...	[more]
A0A6M2ERA4	1.1e-41	77.57	EF-hand domain-containing protein OS=Populus davidiana OX=266767 PE=4 SV=1	[more]

BLAST of Carg03155-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27280.1	5.8e-35	66.04	Calcium-binding EF-hand family protein	[more]
AT5G54490.1	3.2e-33	64.15	pinoid-binding protein 1	[more]
AT2G46600.1	5.1e-31	59.62	Calcium-binding EF-hand family protein	[more]
AT4G23650.1	5.7e-06	34.07	calcium-dependent protein kinase 6	[more]
AT1G66410.1	4.8e-05	34.09	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 70..98 e-value: 0.0026 score: 27.0
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 45..97 e-value: 2.6E-18 score: 65.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 66..101 score: 10.273304
None	No IPR available	GENE3D	1.10.238.10		coord: 12..121 e-value: 2.7E-21 score: 77.6
None	No IPR available	PANTHER	PTHR47319:SF8	CALCIUM-BINDING PROTEIN PBP1-LIKE	coord: 13..119
IPR044205	Calcium-binding protein KIC/PBP1/KRP1	PANTHER	PTHR47319	CALCIUM-BINDING PROTEIN KIC	coord: 13..119
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 79..91
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 29..109

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg03155	Carg03155	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03155-RA:1	Carg03155-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03155-RA:cds	Carg03155-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg03155-RA	Carg03155-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding