Carg03132-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03132-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNEDD8-activating enzyme E1 regulatory subunit
LocationCarg_Chr20: 2941785 .. 2950258 (+)
Sequence length2158
RNA-Seq ExpressionCarg03132-RA
SyntenyCarg03132-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCAGTCTTCACTGCGTCGTTCCCACGCAAATTCAGAAAGCTCATTGAGACTTCAGACAAGAGGAACAAGACTTGAACAAGAGAGGGAGAACAGAGCGCGCGAAGCAAAGAAAATGGCGGAGCCCAAAGTAAAATACGATCGCCAACTCAGGTCCTCCTCATTCCCTCTATCACACCTTCCTCATTCCTCAATCTTCAACTCTTCTTCATATTTCGATTCTTTGCCTCTAAATTCTTGCTCCGTTTTTTGCCCTAACTCTGTACCAGTAGCATACTCCACCTTCAATTTGTCTGTACTTCTTCTCTCGCTAATTTATCTTTAGGTTTATCTGTTCTTTCGTCCAGTGAAAGAAAAGAAGCGCAATGTTTATGAACTAATCCGCATTTGGCGCAAACAAAGTTCGCGCACTTCTTAGTTTCTTGGTGTCGTAGTTACCCAGCAGCTGTTCCCGCTATGATTAACCATTGACATTTTTTTTCTTTATAGGAATTGCTGGAATTTTCGTGATTGCATATATATATATATTTTTTCTTTTATTGTAACTATATATTGCTCGACGATATATAGTTTGTCGGTAAGTTGACAAGTAATGAATTTACATCGGAGCTTAGGTTTTACTCACATCATTGCGGCAACTTAGTTCTTGTAGTATTATCTCTATGGAGAAATGGGTTACCTAGAGTGTTTCTTTCTTTCTTTTTTGGGTCAAATTTATCATTTAGCAAACTTGTTCGACTAAATTTAATCATCTTTATTACCTTTATGTTCTGAAAGCTTTTATCCCGTGATATCGTAGGATCTGGGGTGAACAAGGGCAGGCAGCCCTTGAAAAAGCGAGTATATGCCTTCTCAACTGCGGGCCTACTGGTTCCGAGACATTGAAAAATCTAGTACTTGGAGGGATTGGAAGCATCACTATAGTCGACGGTACCAAGATAGAAGAGGGCGACCTTGGCAACAACTTCATGGGTAAGTAAGTTATATGTCACTGTTGCTTTTGGTAATGGAGCCCGATTTGTAATTGGCAATTGCCTATGGACCTACGAATGATATTTTCTCATAATTTAACTTGAAATTTGAAGTTAAATTGATTATTTGGGAGGACTATCAATTAGAATTCTGGAATTGCCTTTCCATAATAATCTTTTGAAAAATCATTCTGTCAACAATAAAATGAATCTTCTTTCCAATCTGCTGTCCGTATGTACTTGAATAATCTACTTCAATACTAATCAATACTTTTGAGCTATAACAACTTCTGTAAGGAAAGAAAAACATTGTTTATCAACTAAACCTTATCTTGTTTTGTGTGATTATTTTTCTAGTGGACGAATCTAGTATTGGACAGTCAAAGGCAAAGTGTGTATGTGCATTTCTTCAAGAACTAAATGATGCCGTGAAAGCTAAGTTTATAGAAGAGTGTCCGGAGGCATTAATTGAAACAAGGCCATCATTCTTTTCACAGTTTACATTAGTAGTTGCCACACAGGTACTCTGATATACATGCTTAGTTCAATTGATGTTGCCCCACTTTGCTGAAATTCTTGGTTTTTCTCTTGCCTGTCATCTCTTATATCATGTCCTTTGTGCAATTGTTTTCGCATCACTTGGATTTTTAGTTGCATTGGTAAAATTATTATTATTTATAAGAAACTAAAAAGTTTCAAGGAGTGAATAATTTTTAAAGTTTTGGAAGAGGCACACTGAAAAACTGCATGAACCTTGATTAGCTCAAAGCTTTCTTCCCAGGGGTAAGTTTTTCCATAAGAGATGCTTTTCTTACTCTCATACGAAATTCCTCGAGTCATAGATTTAACAATGTTACTCAAAGGGTTCTTGTTGGGAGATATTTTTATCCCTGAAAATTAGTAACCTAAGGAACATATAACAAATTCAAGCCTGTACGGGGGATGCTTTTTGGTTAAATCTCTTATTAGCAAATTGATGGTTGGTAAAAAGGTTAAAAGTGTTTTTTAGTGTAGTTGGCATGCTTAGAAAGCCTCAACAATAGCGGAAAGTGCACAAACTCCAATTTTCTGTTCTTGGTTTTGTTAAATAGTGAGTTTTTTCTTTGGTTACTTGTTTTCTGTAATTTGTCATGGTATGGAATTTTTTTTTTCTCTAAGGTTTTGTTGGAAAGAAAGTCGTATTTGGACACTTTGAATTGCCCTTTTAATGGACACTTCGAATTGCCCTTTTAATGGACACTTCGAATTGCCCTTTTAATGGACACTTTGAATTGCCCTTTTAATCGACACTTTGAATTTATTCAGTTGGTTGAAGAATGGATTGTGAAGCTAGATAAAATCTGTAGGAACGCAAATGTCATATTGGTCGTTGCACGCTCCTACGGACTCACTGGGCTTGTCAGAATCAGTTTAAAGGTAAAGTATTGCTAATGCTACTTCAATTACCCAATTCAAAGAAATAATCATGTGGTATGCATAGAGGGGTTCAAAGTATTTATATTACCATTTCTGCGGAAAAAATGCCTGACACATGATATTTGAATAATCTGGATCCTTTACTAGCCCTTATTAAACTTGTACTTTTCAATTATCATGTATATTTACCTGAATTCCGGCTTAATATCAAATAACTAGTTGTGATGCTTAGCTTGAACTTGGAGAAATAGAGGCTCGTAATTAAAAGCGTTTGCAGGTTGAAATGGTAAATTGCTGACGTAAAACAGCTTAACTGTCACTTCTTTGAGTCCCATATATAAGCTACTTAATGTCTTATGGGGTCTTGTCTGTCACAAATATTTCAATATGGTTCACTTGCACAAACAGTTTTAATGTTTTAAATAAGTTTTTACTTGATGGTTTCAAAAGATAAAATATTGTATATAGTCAAGGGCGCTAAAATAAGATATTTTATCCATGTATATCAGGAGCATACTGTCATTGAGTCAAAGCCCGATCATTTTCTGGATGATCTTCGGTTAAATAACCCATGGCCAGAGCTGAGGAGGTAGAGTTTTTAGTCACATCCTTTCCATCTTTTTAATAGGATGGATTTCATATGTGAATATTGTCTGTTTGCATTAGTTTTTGGTCTATCTAAGCTGTGAAGTTTACTATTAAAGCACAATCATTGATTGGAGAACATAGACGTTTCATTCTTTCAAATTTTGATATACCATGTACTCACCTCTTGACAGGTTTTCTGAAACTATTGATCTCAATATTCCTGACCCTGTTGCACACAAACACACTCCGTATGTTGTTATTCTTGTCAAGATGGCTGAAGAGTGGGCCAAAAGCCACGGTGGCAGTTTACCATCAAGCAGAGAAGAGAAGAAAGAATTCAAGGTTGCCTTTTATTTGCCTTTTTGCAATCAGTAACATGTGATAGTCACTAAAATTGTGGGCTCTTGGACAATGCAGGATCTCCTCAAAGCCAAGATGATTGCAATGGATGAAGATAACTACAGAGAAGCTATTGAAGCTTCGTTCAAAGTCTTTGCTCCGCATGGAATCAGTAAGTGGGCATTTTTTAAAAACATTTAGACTAATTGATCTAGGAACACAAACCTGCTGCTACTTGTTTCGTAGTCTTCCCTTTTGGAGTTGTCTCACATCCTGGGTCTTTTGTTGAAATTTCATCTATTTTCTTCAAAGCAATGCCTAGAAGGGTCCTCTTCCCTGAAGAAGAAATTTGGTTGGAGCTAATTTTTTAGGACCGAATAAGCTCTAGGTCTAGATGGGTTATTTGCTGAATTGTTTGAAAGATGTTGGCGTACTTGCAAGCATGATATTATGGAGAATGGAGATGGTCCATGATTGTTTGAGAGCGGTGGCATTCTTGACATTCTCAATGGAATTATCAATTGTGCCCATACCACAGTAGGTTGGTTCTGAGGTCCTCGACTTCAATCCCTTAACCTCACTAGCTTGGAGATTGAAGGAAAAACAACCTAAAAAAATTATCACATCACAGGACTGGAGATTGAAAAAAAAGACGACCTAAAAGAACAAAAAGAAAACAGATTAACTAAACCCAGCCAGACACAACCTATACTCCAAACACAAGTTTCAGAAGCCCAACCAACCCTGCATCCCAAACGCGGGTTTCAGAAACCTTAGGATTTTTTATTCCCGCCAATCAACCCATTGGTTTCAGGACAATATCTAGAAGGATCCTCACGTGGTTCTGTCTCTAGATTGATCTACCAGGTGCAAAAAAGTGTCGCACACCGTTTCTCCTTTTTCCTGTTTGCTTGCAAAATCTGGGGGAAAAACCTTTCATTCCTTTCATTGGTTCACATCTATTCCAACTAATATCAACACCATGTTCTCGGATTCTGTTCTTTTAAAAAAGGAAAGGAAGTTCTTGTGCATTATGAGAGACTCTTTTAAGCTTCTTACAGTAGAATATTCAAAATATTTAAACATCTTTTCAGGATATTGATTACTGCCCTTACTCGAGTTCCATCATCTGGTTTGTATTTTTAACTAAACTCTTGTGTCAATTGAAAGTTGGAAGTTAAAAAAAAAGAAGAAAGAAAAAGATCTTTTTTGAAAGTTGGAGCTGCCAGTTTAATTTCTCTAGCTCTTCTGAAAGTTAGTTTTCACTTAACGTGGTCCAACTATTTCTGAATGTCATGGAAAGGATTGTGGAGTATCACCTCTTGTTGTACATAGTAGTTGAACGTTTAACATCTTGAGACTGCCTACAATTTTAATGAAATTAGATTTGTCAATTGGAGTGTCTGATGGCGTATTTTAAGGTTTTTGTTCAAATAAAATTTTTTAGTTCCTATAAGGTGACAGATCTACTTAGTGTCTAGAAATATGAGCACCATTATTTATCATTTTCTTCATATCAGGTCCAGATCTGAAACAGATAGTTGATGATTGCTGTGCTGAAGTTGATTCTAATTCATCAGATTTTTGGATATTAGTGGCTGCTTTGAAGGTTACTGAAACTTTTTCACTACTACTTTAGCTCTGTATCTCCAACAATTGATGATTTCTTTTTAGGTGTTATTCGAGTTATGACAATCAGACTTATATAGTACTTGAGGTTCTTAGAAATGACCAGTCACCACCATCTTGCTTAACTATCACATTCTTCTAATTCATATGATGGCTGGATATTTAATTGATTTGTTGCATGTATATTGCTTGATCTATTCTTAACAATCCTTAGATTGGAGGATCTGCTTGTGGATTTACTTGTTTGTTCTCATTATTCCTTTTGAGGGATCAGGAAATGCAAGTGTGATTCACTTGAATTAACACATACTGTTAAATTACCGTAGTAAATTTAATTTCGCTTCTGATTCATTGTTTTATTTTATAATAGCTAAATATCTACGGATTCATGTGGTTTAAATCAATCAATTCTTAAGTAATTCATGCATTACATATGATTCTGTTTAATCATCCAAAATTTCAAAATATATTGTTAAAGAAGTAATTTTGCGATTGACATTATTAATTTATAGTAAATTTATATATTATTATTATTATACTAAAGTAAATACTTAATATTTATTGATTTTAGTAATATCGCCAATAATAACTAATACCTTAATAGTTATTTTTTTTATTGATAAAAAAATTGTAAGGAACAATGTGTTAAAATATATTGTATTGCTGTTACTGCTATTCTTCTCTTTGGTATAGAATGAAATCATTTTGGACTGTAAGTTGAAAAGTCCTCGAGTATCTTTTAAAATTTGGCTGTTTAACCATTATTATTTTGACCCTTGACCTTCGTCTCGCCCTCTCTCCTCTATTTGTCATCTATCTGTTCATTAACGCAAGTATTGATTTGGAAATTCTAACAATATCGTATGCATTACTTCCAAAGTTGTAGCCTGAACTTGATGTAGTTGTACTCCATTTTCCTTTAAAATGTTTCTTTAATTTCTCTGCAGGAGTTCATAGCGAATGAAGGAGGTGGTGAGGCACCCATGGAGGGATCAATACCAGATATGACATCTTCAACTGAGTGAGTGTCTCAATATTCTAGAAAATACCATGAAATTTAACTAACTTATTTATTGACTGAAGGGCTGGATTCTGCCTTTTGTAGGCACTATGTTAATTTGCAAAACATCTATCAAGCTAAGGCCGAGGCTGACTTCATGCTAATAGAGGAAAGAGCAAGGAACATTTTGAAAAAAATTGGCAGAGATCCAAATAGCATCTCTAAAACGACAATCAAAAGCTTCTGTAAAAATGCAAGAAAGCTGAAAGTATGCTCTATTATGATCATTTTTATAATACTTATTGTTCTTTTTGTACAATCTGCTGGTATCCCAAGTTGATAGGACAGGGGCCTTCCAATTTAATCCTGTCATTCTCCATGATATTCTAAGAATCTTCCTTTAAAGAGCCTAATTACGATTTAGCCATCATGACGTATGTGGTCAGAGCTTTTTGAGTTTTTCCTAGGAGTTCTGGATGTATCACAGGACAGATTTCCTCTCTGTCTAAATGGTTCTCTATCTTGTTTAATAATTTTTTAGTTTCTTGATAAATTAAAGAACTACCTATCAGGAATCCACCAATGAATTTTGAATTCACCTTCGCATGTACAAAGCTCCCTTTGCTCAGATAAATGTAGTGTGGTATGATTGTGTAGTGCATAATGGCTGAATATGTTGCTCTCAAAAACTTTATTCTACTTCAACTGAGGCATTTGTGTGGATGCATGCATGCCTACAAATTCGTGCATATACAGTTTTTTTTTTTCTAATGGATTATGGGAAATTTTTGTAGGTTTGCAGATATCGTTCACTTGAGGATGAGTTCAATTCTCCTATCTTCGCTGAGCTACAGAAGTATCTAACTGACGAGGATTTCAGGTTACACTAAATTAGTCAACGTGCTTCTTACTTTGTTTTCTGATGTTACATTTTTACTTTATTCAGTGCAATGATGAATGGACGATATCATATAATTTCATTTGTATTAAAATACGATGTTCTTAGAAATCCTTTCTTTGGTATTTTTAATAGCAATAAATAAATAAATAGATTTAATTACTTATTGGCGTAAAAACTATTTATTATCAATCTTAAATTTGGACAATAATGTTACATCTCTGTTTCTTTATACAAGTATTTGCCCTTTCAACATCTTTGTGCCGATTTTGGTATGTTTCTTTTCAGTAGCTAAATTTCTTTCAGGCAACCAGTTTTACCGGGGAGAGTTGTTTGCTTACTAATTTATTATCTATTTATGCATCCAATATTATTGCAATCAATTTATTATTATTATTTTTTTTGGGTAAGTGCGCTTTCATTGAGAAAACCAATTTTTGTTGCTACATGATAGTTGGATTCGTTTCTCTGCATTTGTTTACCGAAGATCTTGGAACTTCTCTTCAACTGAATAAGTATATATATTAAAAAATAAGTAATCTCTACTCTATTGACATTTATTTCTCTTCTTCCAGCGTGGCAGTTGGATTTTATATTCTGCTTCGAGCTGTTGATCGTTTTGCTGCCAATTACAACAGTTTTCCCGGGCAATTTGATGGGTAGTGTGTCTGATGCTGTTTTGTATGGGATATGTGTTTCTTTAATAATGATGTAGACTTTTAACTAATTGATGCTCTTGCAGTGGGATTGACGAGGACATATCTCGATTGAAAACTACTGCCGTTGGCCTACTCAGTGACTTGGGTTGCAATGGCTTGACTTTATCAGAGGACCTCATTAATGAGATGTGCCGATTCGGTGCGGCAGAGCTCCATGCAGTCGCTGCTTTCATTGGTGGAATTGCATCACAAGAAGTAATCAAGGTAAAGTTATTACTTTCTGGGCCTTTTTTTTTTTTTTTTTTTTTTTTCCATTTCATCTATCATTTGATATTTTACCCCCTGCCTAAAGTGACATTTGCATGAAAGCTTCGGTCTCCTAGAATGACGTATTAAAAAAGGTCCTCTTTTGTCTTCAGCTAATCACCCGACAGTTTGTACCCATGTCTGGAACTTTCGTATTCAATGGCATCGATCACAAGTCGCAGTTATTGTCGCTCTAGTGACTTTTAGACCCATACCTTAAGTTTCCCAGATTCGCCTCGCTTCGATTCTTATTGGCAGACCAGGCATTTTCCCTGGAGGAGAAAGCGCTTACTTCTTTGTTGGAGCTATGGTATGGTACACATTGCACGATTCTATACTTCGGTGTGCAATTACCTTATTAGATCGAATTTTTGGCATAATTTTTCAGAATATCAGAATATCATCGGTAGAACAAAAAAGGGTCACAGGAAAGTTTCCAATGCCTATAACCTTCAATCCTACGAGAGTAGTATGCCCCTTGAAATTGCCTCCTCTCCTGAGTCTCTTGAATCAATTTGGGTCTCAAAAATATTATTATTATGATCATTATGGGGAATTACTGACTAAGGAAAATGGATTATTGTTCTTTTGTTTTTGCCTTTTAATCTTTCTTCCCTTGTTACACTTGCACCTACCGGTGTGCATTGGGTATTAAATATAGCCACTTTT

mRNA sequence

ATTCAGTCTTCACTGCGTCGTTCCCACGCAAATTCAGAAAGCTCATTGAGACTTCAGACAAGAGGAACAAGACTTGAACAAGAGAGGGAGAACAGAGCGCGCGAAGCAAAGAAAATGGCGGAGCCCAAAGTAAAATACGATCGCCAACTCAGGATCTGGGGTGAACAAGGGCAGGCAGCCCTTGAAAAAGCGAGTATATGCCTTCTCAACTGCGGGCCTACTGGTTCCGAGACATTGAAAAATCTAGTACTTGGAGGGATTGGAAGCATCACTATAGTCGACGGTACCAAGATAGAAGAGGGCGACCTTGGCAACAACTTCATGGTGGACGAATCTAGTATTGGACAGTCAAAGGCAAAGTGTGTATGTGCATTTCTTCAAGAACTAAATGATGCCGTGAAAGCTAAGTTTATAGAAGAGTGTCCGGAGGCATTAATTGAAACAAGGCCATCATTCTTTTCACAGTTTACATTAGTAGTTGCCACACAGTTGGTTGAAGAATGGATTGTGAAGCTAGATAAAATCTGTAGGAACGCAAATGTCATATTGGTCGTTGCACGCTCCTACGGACTCACTGGGCTTGTCAGAATCAGTTTAAAGGAGCATACTGTCATTGAGTCAAAGCCCGATCATTTTCTGGATGATCTTCGGTTAAATAACCCATGGCCAGAGCTGAGGAGGTTTTCTGAAACTATTGATCTCAATATTCCTGACCCTGTTGCACACAAACACACTCCGTATGTTGTTATTCTTGTCAAGATGGCTGAAGAGTGGGCCAAAAGCCACGGTGGCAGTTTACCATCAAGCAGAGAAGAGAAGAAAGAATTCAAGGATCTCCTCAAAGCCAAGATGATTGCAATGGATGAAGATAACTACAGAGAAGCTATTGAAGCTTCGTTCAAAGTCTTTGCTCCGCATGGAATCAGTCCAGATCTGAAACAGATAGTTGATGATTGCTGTGCTGAAGTTGATTCTAATTCATCAGATTTTTGGATATTAGTGGCTGCTTTGAAGGAGTTCATAGCGAATGAAGGAGGTGGTGAGGCACCCATGGAGGGATCAATACCAGATATGACATCTTCAACTGAGCACTATGTTAATTTGCAAAACATCTATCAAGCTAAGGCCGAGGCTGACTTCATGCTAATAGAGGAAAGAGCAAGGAACATTTTGAAAAAAATTGGCAGAGATCCAAATAGCATCTCTAAAACGACAATCAAAAGCTTCTGTAAAAATGCAAGAAAGCTGAAAGTTTGCAGATATCGTTCACTTGAGGATGAGTTCAATTCTCCTATCTTCGCTGAGCTACAGAAGTATCTAACTGACGAGGATTTCAGCGTGGCAGTTGGATTTTATATTCTGCTTCGAGCTGTTGATCGTTTTGCTGCCAATTACAACAGTTTTCCCGGGCAATTTGATGGTGGGATTGACGAGGACATATCTCGATTGAAAACTACTGCCGTTGGCCTACTCAGTGACTTGGGTTGCAATGGCTTGACTTTATCAGAGGACCTCATTAATGAGATGTGCCGATTCGGTGCGGCAGAGCTCCATGCAGTCGCTGCTTTCATTGGTGGAATTGCATCACAAGAAGTAATCAAGCTAATCACCCGACAGTTTGTACCCATGTCTGGAACTTTCGTATTCAATGGCATCGATCACAAGTCGCAGTTATTGTCGCTCTAGTGACTTTTAGACCCATACCTTAAGTTTCCCAGATTCGCCTCGCTTCGATTCTTATTGGCAGACCAGGCATTTTCCCTGGAGGAGAAAGCGCTTACTTCTTTGTTGGAGCTATGGTATGGTACACATTGCACGATTCTATACTTCGGTGTGCAATTACCTTATTAGATCGAATTTTTGGCATAATTTTTCAGAATATCAGAATATCATCGGTAGAACAAAAAAGGGTCACAGGAAAGTTTCCAATGCCTATAACCTTCAATCCTACGAGAGTAGTATGCCCCTTGAAATTGCCTCCTCTCCTGAGTCTCTTGAATCAATTTGGGTCTCAAAAATATTATTATTATGATCATTATGGGGAATTACTGACTAAGGAAAATGGATTATTGTTCTTTTGTTTTTGCCTTTTAATCTTTCTTCCCTTGTTACACTTGCACCTACCGGTGTGCATTGGGTATTAAATATAGCCACTTTT

Coding sequence (CDS)

ATGGCGGAGCCCAAAGTAAAATACGATCGCCAACTCAGGATCTGGGGTGAACAAGGGCAGGCAGCCCTTGAAAAAGCGAGTATATGCCTTCTCAACTGCGGGCCTACTGGTTCCGAGACATTGAAAAATCTAGTACTTGGAGGGATTGGAAGCATCACTATAGTCGACGGTACCAAGATAGAAGAGGGCGACCTTGGCAACAACTTCATGGTGGACGAATCTAGTATTGGACAGTCAAAGGCAAAGTGTGTATGTGCATTTCTTCAAGAACTAAATGATGCCGTGAAAGCTAAGTTTATAGAAGAGTGTCCGGAGGCATTAATTGAAACAAGGCCATCATTCTTTTCACAGTTTACATTAGTAGTTGCCACACAGTTGGTTGAAGAATGGATTGTGAAGCTAGATAAAATCTGTAGGAACGCAAATGTCATATTGGTCGTTGCACGCTCCTACGGACTCACTGGGCTTGTCAGAATCAGTTTAAAGGAGCATACTGTCATTGAGTCAAAGCCCGATCATTTTCTGGATGATCTTCGGTTAAATAACCCATGGCCAGAGCTGAGGAGGTTTTCTGAAACTATTGATCTCAATATTCCTGACCCTGTTGCACACAAACACACTCCGTATGTTGTTATTCTTGTCAAGATGGCTGAAGAGTGGGCCAAAAGCCACGGTGGCAGTTTACCATCAAGCAGAGAAGAGAAGAAAGAATTCAAGGATCTCCTCAAAGCCAAGATGATTGCAATGGATGAAGATAACTACAGAGAAGCTATTGAAGCTTCGTTCAAAGTCTTTGCTCCGCATGGAATCAGTCCAGATCTGAAACAGATAGTTGATGATTGCTGTGCTGAAGTTGATTCTAATTCATCAGATTTTTGGATATTAGTGGCTGCTTTGAAGGAGTTCATAGCGAATGAAGGAGGTGGTGAGGCACCCATGGAGGGATCAATACCAGATATGACATCTTCAACTGAGCACTATGTTAATTTGCAAAACATCTATCAAGCTAAGGCCGAGGCTGACTTCATGCTAATAGAGGAAAGAGCAAGGAACATTTTGAAAAAAATTGGCAGAGATCCAAATAGCATCTCTAAAACGACAATCAAAAGCTTCTGTAAAAATGCAAGAAAGCTGAAAGTTTGCAGATATCGTTCACTTGAGGATGAGTTCAATTCTCCTATCTTCGCTGAGCTACAGAAGTATCTAACTGACGAGGATTTCAGCGTGGCAGTTGGATTTTATATTCTGCTTCGAGCTGTTGATCGTTTTGCTGCCAATTACAACAGTTTTCCCGGGCAATTTGATGGTGGGATTGACGAGGACATATCTCGATTGAAAACTACTGCCGTTGGCCTACTCAGTGACTTGGGTTGCAATGGCTTGACTTTATCAGAGGACCTCATTAATGAGATGTGCCGATTCGGTGCGGCAGAGCTCCATGCAGTCGCTGCTTTCATTGGTGGAATTGCATCACAAGAAGTAATCAAGCTAATCACCCGACAGTTTGTACCCATGTCTGGAACTTTCGTATTCAATGGCATCGATCACAAGTCGCAGTTATTGTCGCTCTAG

Protein sequence

MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALKEFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Homology
BLAST of Carg03132-RA vs. NCBI nr
Match: XP_022944430.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita moschata] >KAG7010733.1 NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1042.3 bits (2694), Expect = 1.4e-300
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD
Sbjct: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP
Sbjct: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV
Sbjct: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Sbjct: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. NCBI nr
Match: XP_023512938.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.4e-299
Identity = 521/523 (99.62%), Postives = 522/523 (99.81%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRFSETID+NIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD
Sbjct: 181 NNPWPELRRFSETIDVNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDD CAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP
Sbjct: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV
Sbjct: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Sbjct: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. NCBI nr
Match: XP_022986290.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita maxima])

HSP 1 Score: 1036.2 bits (2678), Expect = 9.8e-299
Identity = 520/523 (99.43%), Postives = 521/523 (99.62%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSIT+VDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITLVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD
Sbjct: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDD CAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP
Sbjct: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIK FCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV
Sbjct: 361 NSISKTTIKGFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Sbjct: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. NCBI nr
Match: KAG6570885.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1032.7 bits (2669), Expect = 1.1e-297
Identity = 517/517 (100.00%), Postives = 517/517 (100.00%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD
Sbjct: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP
Sbjct: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV
Sbjct: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHK 518
           AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHK
Sbjct: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHK 517

BLAST of Carg03132-RA vs. NCBI nr
Match: XP_038901338.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Benincasa hispida])

HSP 1 Score: 1007.3 bits (2603), Expect = 4.9e-290
Identity = 503/523 (96.18%), Postives = 512/523 (97.90%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           +EGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTL
Sbjct: 61  DEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEEYPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRF+ETIDLN PDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKK FKD
Sbjct: 181 NNPWPELRRFAETIDLNAPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKAFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIA DEDNY+EAIEASFKVFAPHGISPDLKQIVDD CAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIATDEDNYKEAIEASFKVFAPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADF +IEERARNILKK+GRDP
Sbjct: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFKIIEERARNILKKVGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIKSFCKNARKLKVCRYRSLEDEF+SP   ELQKYLTDEDFSVAVGFY+LLRAV
Sbjct: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFSSPFLPELQKYLTDEDFSVAVGFYLLLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLL+DLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLNDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
            VAAF GGIASQEVIKLITRQFVPMSGTF+FNGIDHKSQLLSL
Sbjct: 481 VVAAFTGGIASQEVIKLITRQFVPMSGTFIFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. ExPASy Swiss-Prot
Match: P42744 (NEDD8-activating enzyme E1 regulatory subunit AXR1 OS=Arabidopsis thaliana OX=3702 GN=AXR1 PE=1 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 1.9e-244
Identity = 404/524 (77.10%), Postives = 471/524 (89.89%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           M EPK KYDRQLRIWGE GQAALE+ASICLLNCGPTGSE LKNLVLGG+GSIT+VDG+K+
Sbjct: 17  MVEPKTKYDRQLRIWGEVGQAALEEASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKV 76

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           + GDLGNNFMVD  S+GQSKAK VCAFLQELND+V AKFIEE P+ LI T PSFFSQFTL
Sbjct: 77  QFGDLGNNFMVDAKSVGQSKAKSVCAFLQELNDSVNAKFIEENPDTLITTNPSFFSQFTL 136

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           V+ATQLVE+ ++KLD+ICR+ANV LV+ RSYGL G VRIS+KEH +I+SKPDHFLDDLRL
Sbjct: 137 VIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRL 196

Query: 181 NNPWPELRRFSETIDLNIPDP-VAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFK 240
           NNPWPEL+ F ETIDLN+ +P  AHKH PYVVILVKMAEEWA+SH G+LPS+REEKKEFK
Sbjct: 197 NNPWPELKSFVETIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHSGNLPSTREEKKEFK 256

Query: 241 DLLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAAL 300
           DL+K+KM++ DEDNY+EAIEA+FKVFAP GIS +++++++D CAEV+SNSS FW++VAAL
Sbjct: 257 DLVKSKMVSTDEDNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEVNSNSSAFWVMVAAL 316

Query: 301 KEFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRD 360
           KEF+ NEGGGEAP+EGSIPDMTSSTEHY+NLQ IY AKAEADF++IEER +NILKKIGRD
Sbjct: 317 KEFVLNEGGGEAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVIEERVKNILKKIGRD 376

Query: 361 PNSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRA 420
           P+SI K TIKSFCKNARKLK+CRYR +EDEF +P   E+QKYL DED+S A+GFYILLRA
Sbjct: 377 PSSIPKPTIKSFCKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADEDYSGAMGFYILLRA 436

Query: 421 VDRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAEL 480
            DRFAANYN FPGQFDGG+DEDISRLKTTA+ LL+DLGCNG  L +DLI+EMCRFGA+E+
Sbjct: 437 ADRFAANYNKFPGQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPDDLIHEMCRFGASEI 496

Query: 481 HAVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           H V+AF+GGIASQEVIKL+T+QFVPM GT++FNGIDHKSQLL L
Sbjct: 497 HVVSAFVGGIASQEVIKLVTKQFVPMLGTYIFNGIDHKSQLLKL 540

BLAST of Carg03132-RA vs. ExPASy Swiss-Prot
Match: Q9ZV69 (NEDD8-activating enzyme E1 regulatory subunit AXL OS=Arabidopsis thaliana OX=3702 GN=AXL1 PE=1 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 8.2e-232
Identity = 385/523 (73.61%), Postives = 453/523 (86.62%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPK KYDRQLRIWGE GQ+ALE ASICLLNCGPTGSE LKNLV+GGIGSITIVDG+K+
Sbjct: 1   MAEPKTKYDRQLRIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKV 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           E GDLGNNFMVD  S+GQS+AK VC FLQELND+VKA F+EE P+ LI T PSFFSQFTL
Sbjct: 61  EIGDLGNNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           V+ATQLVE+ +VKLD+ICR ANV+LV+ARSYGLTG VRIS+KEHT IE+KPDH LDDLRL
Sbjct: 121 VIATQLVEDSMVKLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           N+PWPEL+ + E+IDLN+ +P AHKH PYVVILVK+AEEWA+ H G+LPS+REEK EFKD
Sbjct: 181 NSPWPELKSYVESIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           L+K+KM++ DE+NY+EA+ A+FKVFAP GIS +++ I  D CAEV SNSSDFW++VAALK
Sbjct: 241 LVKSKMVSADEENYKEALLAAFKVFAPTGISQEIQDINHDSCAEVGSNSSDFWVMVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFI+NEGGGE P+EGS+PDM SSTEHY+NLQ IY +KAEADF+ +E+R ++IL K+G+DP
Sbjct: 301 EFISNEGGGEVPLEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVGQDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           +SISK TIKSFCKNARKLKVCRYR++EDEF SP   EL KYL DE++S A+GFYILLRAV
Sbjct: 361 SSISKPTIKSFCKNARKLKVCRYRTIEDEFKSPSTTELHKYLADENYSGAIGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFA  Y  FPGQFDG  DED S+LKT A+ LLS++GC+G  L E+L NEMCRFGAAE+H
Sbjct: 421 DRFAGTYKKFPGQFDGSTDEDASQLKTIALSLLSEMGCDGYELQEELYNEMCRFGAAEIH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
            VAA IGGI SQEVIKLIT+QFVP  GTF+FNGIDHKSQ L+L
Sbjct: 481 VVAALIGGITSQEVIKLITKQFVPKRGTFIFNGIDHKSQSLTL 523

BLAST of Carg03132-RA vs. ExPASy Swiss-Prot
Match: Q8VBW6 (NEDD8-activating enzyme E1 regulatory subunit OS=Mus musculus OX=10090 GN=Nae1 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 3.1e-122
Identity = 222/535 (41.50%), Postives = 336/535 (62.80%), Query Frame = 0

Query: 1   MAEP-----KVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIV 60
           MA+P     + KYDRQLR+WG+ GQ ALE A +CL+N   TG+E LKNLVL GIGS TI+
Sbjct: 1   MAQPGKILKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTII 60

Query: 61  DGTKIEEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFF 120
           DG  +   D GNNF + +SSIG+++A+    FLQELN  V   F+EE PE L++  PSFF
Sbjct: 61  DGNLVSGEDAGNNFFLQKSSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFF 120

Query: 121 SQFTLVVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFL 180
            +FT+VVATQL+E  +++L  +  N+ + L++ R+YGL G +RI +KEH VIES PD+ L
Sbjct: 121 CRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNAL 180

Query: 181 DDLRLNNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEK 240
           +DLRL+ P+PELR   ++ DL+  +   H HTP++VI+ K   +W     G +P S +EK
Sbjct: 181 EDLRLDKPFPELREHLQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYNETNGRIPKSYKEK 240

Query: 241 KEFKDLLKAKMI------AMDEDNYREAIEASFKVFAPHGISPDLKQIV-DDCCAEVDSN 300
           ++F+DL++  ++        DE+N+ EAI+          I   ++ I  DD C  +   
Sbjct: 241 EDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQ 300

Query: 301 SSDFWILVAALKEFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEER 360
           +  FWIL  ALKEF+A EG G  P+ G+IPDM + +  Y+ LQN+Y+ KA+ D   +   
Sbjct: 301 TPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAVGNH 360

Query: 361 ARNILKKIGRDPNSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFS 420
              +L+ +G+ P SIS+  +K  C N+  L+V R RSL +E+      + +   + ++  
Sbjct: 361 VAKLLQSVGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTVNKDEIISSMDNPD 420

Query: 421 VAVGFYILLRAVDRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLI 480
             +  Y++LRAVDRF   +  +PG  +  ++EDI +LK+   G L + G + + + +D +
Sbjct: 421 NEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLS-VMVKDDYV 480

Query: 481 NEMCRFGAAELHAVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           +E CR+GAAE H +AAF+GG A+QEVIK+IT+QFV  + T++++G+   S    L
Sbjct: 481 HEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL 534

BLAST of Carg03132-RA vs. ExPASy Swiss-Prot
Match: Q6NTW6 (NEDD8-activating enzyme E1 regulatory subunit OS=Xenopus laevis OX=8355 GN=nae1 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 3.1e-122
Identity = 222/524 (42.37%), Postives = 335/524 (63.93%), Query Frame = 0

Query: 7   KYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLG 66
           KYDRQLR+WG+ GQ ALE A +CL+N   TG+E LKNLVL GIG  TIVDG  +   D+G
Sbjct: 11  KYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVG 70

Query: 67  NNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQL 126
           NNF + + SIG+++A+     LQELND V   F+ E PE L++  PSFF +FT+V++TQL
Sbjct: 71  NNFFLQKESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQL 130

Query: 127 VEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPE 186
            E  +++L +   ++N+ L+V R+YG  G +RI +KEHTVIES PD+ L+DLRL+ P+PE
Sbjct: 131 PESTLLRLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPE 190

Query: 187 LRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKM 246
           LR   ++ DL+  +   H HTP+++++ K  ++W   +GG +P S +EK+ F+DL++  +
Sbjct: 191 LREHLQSYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQGI 250

Query: 247 ------IAMDEDNYREAIEASFKVFAPHGISPDLKQIV-DDCCAEVDSNSSDFWILVAAL 306
                 +  DE+N+ EAI+          +S  +++I+ DD C  +   S+ FWIL  A+
Sbjct: 251 LKNENGVPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAV 310

Query: 307 KEFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRD 366
           KEF+A EG G  P+ G+IPDM + ++ ++ LQNIY+ KA+ D   +E     +L+ +GR 
Sbjct: 311 KEFMAMEGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRP 370

Query: 367 PNSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRA 426
           P SIS+  I+ FC+N   L+V R RSLE+E+      +       E+    +  Y++LRA
Sbjct: 371 PESISERDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPDNEIVLYLMLRA 430

Query: 427 VDRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAEL 486
           VDRF      +PG ++  I+ DI +LK+   GLL + G + LT+ ++ I E CR+GAAE 
Sbjct: 431 VDRFQKQLGRYPGIYNYQIESDIGKLKSCLNGLLQEYGLS-LTVKDEYIQEFCRYGAAEP 490

Query: 487 HAVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           H +A+F+GG A+QE IK+IT+QFV  + TF++N +   S    L
Sbjct: 491 HTIASFLGGAAAQEAIKIITKQFVIFNNTFIYNAMLQTSATFQL 533

BLAST of Carg03132-RA vs. ExPASy Swiss-Prot
Match: Q54JM3 (NEDD8-activating enzyme E1 regulatory subunit OS=Dictyostelium discoideum OX=44689 GN=nae1 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 4.1e-122
Identity = 224/509 (44.01%), Postives = 316/509 (62.08%), Query Frame = 0

Query: 7   KYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLG 66
           KYDRQLR+WGE GQ+ LE++ I LLN   TG+ETLKNLVL GIGS T+VD  K+ E DLG
Sbjct: 10  KYDRQLRLWGEDGQSKLERSHILLLNGSATGTETLKNLVLPGIGSFTVVDNKKVTESDLG 69

Query: 67  NNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQL 126
           NNF V+ SS+G+ +A  VC  L+ELND VK   +EECP  LI    SFF  F+LVVA +L
Sbjct: 70  NNFFVERSSLGKPRATVVCELLRELNDRVKGFSVEECPIHLINNNISFFKDFSLVVANRL 129

Query: 127 VEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPE 186
            EE ++ L +     N+ L++  SYG  G +RIS  EH +IESKPD  +DDLR+ NP+ +
Sbjct: 130 SEEALLTLSQYLTEQNIPLLITNSYGYIGYLRISTPEHQIIESKPDDPIDDLRIYNPFKQ 189

Query: 187 LRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKM 246
           L   ++ ++L+  +   H H PYV++L+K  +EW  +H   +P +R EK EFK    +  
Sbjct: 190 LVDMADALELDKLNTQQHSHVPYVLLLIKFLKEWRSTHNDKMPETRAEKDEFKKFFNSHS 249

Query: 247 IAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALKEFIANE 306
            + DE N+ E I+   K   P  +  D++ ++ D    +  NS DFW+LVAALKEF+ N 
Sbjct: 250 WSADEMNFVEGIQNLLKYIQPPRVPGDVQNLLKDPKTNITENSDDFWVLVAALKEFMTN- 309

Query: 307 GGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKT 366
                P+ G++PDMTS T +++ LQ  YQ KA AD          IL K+G+  +SIS  
Sbjct: 310 NDNTLPLHGNVPDMTSETHNFIQLQKGYQEKALADLSEFSGYVDQILTKVGK--SSISSD 369

Query: 367 TIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAAN 426
            +K FCKN R L + RYR++ +E+N P    ++  L   D    + FYILLR +D+F   
Sbjct: 370 LVKKFCKNTRFLNIIRYRTISEEYNQPKTNLIKSELEQAD--TVMVFYILLRGIDKFYKT 429

Query: 427 YNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFI 486
           Y+ +PG  D   + DI  LKT     L+++  +   + +D I E  RFG +ELH +A+ +
Sbjct: 430 YHKYPGSSD-DFESDIPLLKTVITQYLAEINISNDLVKDDYIAEFVRFGGSELHNIASLM 489

Query: 487 GGIASQEVIKLITRQFVPMSGTFVFNGID 516
           GG+ SQE+IKLIT Q+ P++ TF+FNGI+
Sbjct: 490 GGVTSQEIIKLITHQYTPLNNTFIFNGIN 512

BLAST of Carg03132-RA vs. ExPASy TrEMBL
Match: A0A6J1FYN4 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita moschata OX=3662 GN=LOC111448883 PE=3 SV=1)

HSP 1 Score: 1042.3 bits (2694), Expect = 6.6e-301
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD
Sbjct: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP
Sbjct: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV
Sbjct: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Sbjct: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. ExPASy TrEMBL
Match: A0A6J1JDN6 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita maxima OX=3661 GN=LOC111484081 PE=3 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 4.7e-299
Identity = 520/523 (99.43%), Postives = 521/523 (99.62%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSIT+VDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITLVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD
Sbjct: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDD CAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP
Sbjct: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIK FCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV
Sbjct: 361 NSISKTTIKGFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Sbjct: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. ExPASy TrEMBL
Match: A0A0A0LMV7 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis sativus OX=3659 GN=Csa_2G418860 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.4e-289
Identity = 503/523 (96.18%), Postives = 511/523 (97.71%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWG+QGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGDQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEEYPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRF+ETIDLN PDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKK FKD
Sbjct: 181 NNPWPELRRFAETIDLNAPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKAFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNY+EAIEASFKVFAP GIS DLKQIV+D CAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYKEAIEASFKVFAPRGISSDLKQIVNDSCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFI NEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADF +IEERARNILKKIGRDP
Sbjct: 301 EFIVNEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFKIIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTT+KSFCKNARKL+VCRYRSLEDEFNSPI  ELQKYLTDEDFSVAVGFY+LLRAV
Sbjct: 361 NSISKTTVKSFCKNARKLRVCRYRSLEDEFNSPIVPELQKYLTDEDFSVAVGFYLLLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
            VAAF GGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Sbjct: 481 VVAAFTGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. ExPASy TrEMBL
Match: A0A6J1CH54 (NEDD8-activating enzyme E1 regulatory subunit OS=Momordica charantia OX=3673 GN=LOC111011149 PE=3 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 5.8e-289
Identity = 497/523 (95.03%), Postives = 512/523 (97.90%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTK+
Sbjct: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKV 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQL EEWIVKLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLGEEWIVKLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRF+E+ID+N+PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKK FKD
Sbjct: 181 NNPWPELRRFAESIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNYREAIEASFKVF PHGISPDLKQIVDD CAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFI NEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFM+I++RARNILKKIGRDP
Sbjct: 301 EFIVNEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTT KSFCKNARKLKVCRYRSLEDEFNSP+F ELQKYLTDEDFSVAVGFYILLRAV
Sbjct: 361 NSISKTTTKSFCKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
            VAAFIGGIASQEVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 481 TVAAFIGGIASQEVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. ExPASy TrEMBL
Match: A0A1S3C1I6 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis melo OX=3656 GN=LOC103495961 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.0e-285
Identity = 499/523 (95.41%), Postives = 505/523 (96.56%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPKVKYDRQLRIWG+QGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI
Sbjct: 1   MAEPKVKYDRQLRIWGDQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRP FFSQFTL
Sbjct: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEEYPEALIETRPWFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           VVATQLVEEWIVKLD ICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL
Sbjct: 121 VVATQLVEEWIVKLDNICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           NNPWPELRRF+ETIDLN  DPVAHKHTPYVVILVKMAEEWAKSHGGSLPS REEKK FKD
Sbjct: 181 NNPWPELRRFAETIDLNASDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSGREEKKAFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           LLKAKMIAMDEDNY+EAIEASFKVFAP GIS DLKQIVDD CAEVDSNSSDFWILVAALK
Sbjct: 241 LLKAKMIAMDEDNYKEAIEASFKVFAPRGISSDLKQIVDDSCAEVDSNSSDFWILVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFI NEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADF +IEERARNILKKIGRDP
Sbjct: 301 EFIVNEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFKIIEERARNILKKIGRDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           NSISKTTIKSFCKNARKL+VCRYRSL DEFNSPI  ELQK LTDEDFSVAVGFY+LLRAV
Sbjct: 361 NSISKTTIKSFCKNARKLRVCRYRSLADEFNSPIVPELQKNLTDEDFSVAVGFYLLLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFAANYNSFPGQFDGGIDEDISRLKTTAV LLSDLGCNGLTLSEDLINEMCRFGAAELH
Sbjct: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVSLLSDLGCNGLTLSEDLINEMCRFGAAELH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
            VAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL
Sbjct: 481 VVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of Carg03132-RA vs. TAIR 10
Match: AT1G05180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 846.3 bits (2185), Expect = 1.3e-245
Identity = 404/524 (77.10%), Postives = 471/524 (89.89%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           M EPK KYDRQLRIWGE GQAALE+ASICLLNCGPTGSE LKNLVLGG+GSIT+VDG+K+
Sbjct: 17  MVEPKTKYDRQLRIWGEVGQAALEEASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKV 76

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           + GDLGNNFMVD  S+GQSKAK VCAFLQELND+V AKFIEE P+ LI T PSFFSQFTL
Sbjct: 77  QFGDLGNNFMVDAKSVGQSKAKSVCAFLQELNDSVNAKFIEENPDTLITTNPSFFSQFTL 136

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           V+ATQLVE+ ++KLD+ICR+ANV LV+ RSYGL G VRIS+KEH +I+SKPDHFLDDLRL
Sbjct: 137 VIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRL 196

Query: 181 NNPWPELRRFSETIDLNIPDP-VAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFK 240
           NNPWPEL+ F ETIDLN+ +P  AHKH PYVVILVKMAEEWA+SH G+LPS+REEKKEFK
Sbjct: 197 NNPWPELKSFVETIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHSGNLPSTREEKKEFK 256

Query: 241 DLLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAAL 300
           DL+K+KM++ DEDNY+EAIEA+FKVFAP GIS +++++++D CAEV+SNSS FW++VAAL
Sbjct: 257 DLVKSKMVSTDEDNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEVNSNSSAFWVMVAAL 316

Query: 301 KEFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRD 360
           KEF+ NEGGGEAP+EGSIPDMTSSTEHY+NLQ IY AKAEADF++IEER +NILKKIGRD
Sbjct: 317 KEFVLNEGGGEAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVIEERVKNILKKIGRD 376

Query: 361 PNSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRA 420
           P+SI K TIKSFCKNARKLK+CRYR +EDEF +P   E+QKYL DED+S A+GFYILLRA
Sbjct: 377 PSSIPKPTIKSFCKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADEDYSGAMGFYILLRA 436

Query: 421 VDRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAEL 480
            DRFAANYN FPGQFDGG+DEDISRLKTTA+ LL+DLGCNG  L +DLI+EMCRFGA+E+
Sbjct: 437 ADRFAANYNKFPGQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPDDLIHEMCRFGASEI 496

Query: 481 HAVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           H V+AF+GGIASQEVIKL+T+QFVPM GT++FNGIDHKSQLL L
Sbjct: 497 HVVSAFVGGIASQEVIKLVTKQFVPMLGTYIFNGIDHKSQLLKL 540

BLAST of Carg03132-RA vs. TAIR 10
Match: AT2G32410.1 (AXR1-like )

HSP 1 Score: 804.3 bits (2076), Expect = 5.8e-233
Identity = 385/523 (73.61%), Postives = 453/523 (86.62%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPK KYDRQLRIWGE GQ+ALE ASICLLNCGPTGSE LKNLV+GGIGSITIVDG+K+
Sbjct: 1   MAEPKTKYDRQLRIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKV 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           E GDLGNNFMVD  S+GQS+AK VC FLQELND+VKA F+EE P+ LI T PSFFSQFTL
Sbjct: 61  EIGDLGNNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           V+ATQLVE+ +VKLD+ICR ANV+LV+ARSYGLTG VRIS+KEHT IE+KPDH LDDLRL
Sbjct: 121 VIATQLVEDSMVKLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           N+PWPEL+ + E+IDLN+ +P AHKH PYVVILVK+AEEWA+ H G+LPS+REEK EFKD
Sbjct: 181 NSPWPELKSYVESIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           L+K+KM++ DE+NY+EA+ A+FKVFAP GIS +++ I  D CAEV SNSSDFW++VAALK
Sbjct: 241 LVKSKMVSADEENYKEALLAAFKVFAPTGISQEIQDINHDSCAEVGSNSSDFWVMVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFI+NEGGGE P+EGS+PDM SSTEHY+NLQ IY +KAEADF+ +E+R ++IL K+G+DP
Sbjct: 301 EFISNEGGGEVPLEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVGQDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAV 420
           +SISK TIKSFCKNARKLKVCRYR++EDEF SP   EL KYL DE++S A+GFYILLRAV
Sbjct: 361 SSISKPTIKSFCKNARKLKVCRYRTIEDEFKSPSTTELHKYLADENYSGAIGFYILLRAV 420

Query: 421 DRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH 480
           DRFA  Y  FPGQFDG  DED S+LKT A+ LLS++GC+G  L E+L NEMCRFGAAE+H
Sbjct: 421 DRFAGTYKKFPGQFDGSTDEDASQLKTIALSLLSEMGCDGYELQEELYNEMCRFGAAEIH 480

Query: 481 AVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
            VAA IGGI SQEVIKLIT+QFVP  GTF+FNGIDHKSQ L+L
Sbjct: 481 VVAALIGGITSQEVIKLITKQFVPKRGTFIFNGIDHKSQSLTL 523

BLAST of Carg03132-RA vs. TAIR 10
Match: AT1G05180.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 654.8 bits (1688), Expect = 5.7e-188
Identity = 312/418 (74.64%), Postives = 372/418 (89.00%), Query Frame = 0

Query: 107 LIETRPSFFSQFTLVVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTV 166
           L   +P+ F  FTLV+ATQLVE+ ++KLD+ICR+ANV LV+ RSYGL G VRIS+KEH +
Sbjct: 21  LANQKPNLF--FTLVIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPI 80

Query: 167 IESKPDHFLDDLRLNNPWPELRRFSETIDLNIPDP-VAHKHTPYVVILVKMAEEWAKSHG 226
           I+SKPDHFLDDLRLNNPWPEL+ F ETIDLN+ +P  AHKH PYVVILVKMAEEWA+SH 
Sbjct: 81  IDSKPDHFLDDLRLNNPWPELKSFVETIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHS 140

Query: 227 GSLPSSREEKKEFKDLLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEV 286
           G+LPS+REEKKEFKDL+K+KM++ DEDNY+EAIEA+FKVFAP GIS +++++++D CAEV
Sbjct: 141 GNLPSTREEKKEFKDLVKSKMVSTDEDNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEV 200

Query: 287 DSNSSDFWILVAALKEFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLI 346
           +SNSS FW++VAALKEF+ NEGGGEAP+EGSIPDMTSSTEHY+NLQ IY AKAEADF++I
Sbjct: 201 NSNSSAFWVMVAALKEFVLNEGGGEAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVI 260

Query: 347 EERARNILKKIGRDPNSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDE 406
           EER +NILKKIGRDP+SI K TIKSFCKNARKLK+CRYR +EDEF +P   E+QKYL DE
Sbjct: 261 EERVKNILKKIGRDPSSIPKPTIKSFCKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADE 320

Query: 407 DFSVAVGFYILLRAVDRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSE 466
           D+S A+GFYILLRA DRFAANYN FPGQFDGG+DEDISRLKTTA+ LL+DLGCNG  L +
Sbjct: 321 DYSGAMGFYILLRAADRFAANYNKFPGQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPD 380

Query: 467 DLINEMCRFGAAELHAVAAFIGGIASQEVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 524
           DLI+EMCRFGA+E+H V+AF+GGIASQEVIKL+T+QFVPM GT++FNGIDHKSQLL L
Sbjct: 381 DLIHEMCRFGASEIHVVSAFVGGIASQEVIKLVTKQFVPMLGTYIFNGIDHKSQLLKL 436

BLAST of Carg03132-RA vs. TAIR 10
Match: AT2G32410.2 (AXR1-like )

HSP 1 Score: 633.3 bits (1632), Expect = 1.8e-181
Identity = 301/411 (73.24%), Postives = 360/411 (87.59%), Query Frame = 0

Query: 1   MAEPKVKYDRQLRIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKI 60
           MAEPK KYDRQLRIWGE GQ+ALE ASICLLNCGPTGSE LKNLV+GGIGSITIVDG+K+
Sbjct: 1   MAEPKTKYDRQLRIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKV 60

Query: 61  EEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTL 120
           E GDLGNNFMVD  S+GQS+AK VC FLQELND+VKA F+EE P+ LI T PSFFSQFTL
Sbjct: 61  EIGDLGNNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTL 120

Query: 121 VVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRL 180
           V+ATQLVE+ +VKLD+ICR ANV+LV+ARSYGLTG VRIS+KEHT IE+KPDH LDDLRL
Sbjct: 121 VIATQLVEDSMVKLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRL 180

Query: 181 NNPWPELRRFSETIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKD 240
           N+PWPEL+ + E+IDLN+ +P AHKH PYVVILVK+AEEWA+ H G+LPS+REEK EFKD
Sbjct: 181 NSPWPELKSYVESIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKD 240

Query: 241 LLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALK 300
           L+K+KM++ DE+NY+EA+ A+FKVFAP GIS +++ I  D CAEV SNSSDFW++VAALK
Sbjct: 241 LVKSKMVSADEENYKEALLAAFKVFAPTGISQEIQDINHDSCAEVGSNSSDFWVMVAALK 300

Query: 301 EFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDP 360
           EFI+NEGGGE P+EGS+PDM SSTEHY+NLQ IY +KAEADF+ +E+R ++IL K+G+DP
Sbjct: 301 EFISNEGGGEVPLEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVGQDP 360

Query: 361 NSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAV 412
           +SISK TIKSFCKNARKLKVCRYR++EDEF SP   EL KYL DE++S ++
Sbjct: 361 SSISKPTIKSFCKNARKLKVCRYRTIEDEFKSPSTTELHKYLADENYSCSI 411

BLAST of Carg03132-RA vs. TAIR 10
Match: AT1G05180.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 610.9 bits (1574), Expect = 9.5e-175
Identity = 295/407 (72.48%), Postives = 356/407 (87.47%), Query Frame = 0

Query: 107 LIETRPSFFSQFTLVVATQLVEEWIVKLDKICRNANVILVVARSYGLTGLVRISLKEHTV 166
           L   +P+ F  FTLV+ATQLVE+ ++KLD+ICR+ANV LV+ RSYGL G VRIS+KEH +
Sbjct: 21  LANQKPNLF--FTLVIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPI 80

Query: 167 IESKPDHFLDDLRLNNPWPELRRFSETIDLNIPDP-VAHKHTPYVVILVKMAEEWAKSHG 226
           I+SKPDHFLDDLRLNNPWPEL+ F ETIDLN+ +P  AHKH PYVVILVKMAEEWA+SH 
Sbjct: 81  IDSKPDHFLDDLRLNNPWPELKSFVETIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHS 140

Query: 227 GSLPSSREEKKEFKDLLKAKMIAMDEDNYREAIEASFKVFAPHGISPDLKQIVDDCCAEV 286
           G+LPS+REEKKEFKDL+K+KM++ DEDNY+EAIEA+FKVFAP GIS +++++++D CAEV
Sbjct: 141 GNLPSTREEKKEFKDLVKSKMVSTDEDNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEV 200

Query: 287 DSNSSDFWILVAALKEFIANEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLI 346
           +SNSS FW++VAALKEF+ NEGGGEAP+EGSIPDMTSSTEHY+NLQ IY AKAEADF++I
Sbjct: 201 NSNSSAFWVMVAALKEFVLNEGGGEAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVI 260

Query: 347 EERARNILKKIGRDPNSISKTTIKSFCKNARKLKVCRYRSLEDEFNSPIFAELQKYLTDE 406
           EER +NILKKIGRDP+SI K TIKSFCKNARKLK+CRYR +EDEF +P   E+QKYL DE
Sbjct: 261 EERVKNILKKIGRDPSSIPKPTIKSFCKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADE 320

Query: 407 DFSVAVGFYILLRAVDRFAANYNSFPGQFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSE 466
           D+S A+GFYILLRA DRFAANYN FPGQFDGG+DEDISRLKTTA+ LL+DLGCNG  L +
Sbjct: 321 DYSGAMGFYILLRAADRFAANYNKFPGQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPD 380

Query: 467 DLINEMCRFGAAELHAVAAFIGGIASQEVIKLITRQFVPMSGTFVFN 513
           DLI+EMCRFGA+E+H V+AF+GGIASQEVIK+   +F+ +S  F F+
Sbjct: 381 DLIHEMCRFGASEIHVVSAFVGGIASQEVIKV---RFIFLSHCFKFS 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022944430.11.4e-300100.00NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita moschata] >KAG7010... [more]
XP_023512938.14.4e-29999.62NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita pepo subsp. pepo][more]
XP_022986290.19.8e-29999.43NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita maxima][more]
KAG6570885.11.1e-297100.00NEDD8-activating enzyme E1 regulatory subunit AXR1, partial [Cucurbita argyrospe... [more]
XP_038901338.14.9e-29096.18NEDD8-activating enzyme E1 regulatory subunit AXR1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P427441.9e-24477.10NEDD8-activating enzyme E1 regulatory subunit AXR1 OS=Arabidopsis thaliana OX=37... [more]
Q9ZV698.2e-23273.61NEDD8-activating enzyme E1 regulatory subunit AXL OS=Arabidopsis thaliana OX=370... [more]
Q8VBW63.1e-12241.50NEDD8-activating enzyme E1 regulatory subunit OS=Mus musculus OX=10090 GN=Nae1 P... [more]
Q6NTW63.1e-12242.37NEDD8-activating enzyme E1 regulatory subunit OS=Xenopus laevis OX=8355 GN=nae1 ... [more]
Q54JM34.1e-12244.01NEDD8-activating enzyme E1 regulatory subunit OS=Dictyostelium discoideum OX=446... [more]
Match NameE-valueIdentityDescription
A0A6J1FYN46.6e-301100.00NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JDN64.7e-29999.43NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0LMV73.4e-28996.18NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A6J1CH545.8e-28995.03NEDD8-activating enzyme E1 regulatory subunit OS=Momordica charantia OX=3673 GN=... [more]
A0A1S3C1I63.0e-28595.41NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT1G05180.11.3e-24577.10NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G32410.15.8e-23373.61AXR1-like [more]
AT1G05180.25.7e-18874.64NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G32410.21.8e-18173.24AXR1-like [more]
AT1G05180.39.5e-17572.48NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 8..501
e-value: 8.7E-23
score: 80.9
NoneNo IPR availableGENE3D3.40.50.720coord: 2..288
e-value: 7.4E-64
score: 217.9
coord: 360..523
e-value: 9.4E-37
score: 128.9
NoneNo IPR availablePANTHERPTHR10953:SF217NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT AXR1coord: 2..523
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 2..523
NoneNo IPR availableCDDcd01493APPBP1_RUBcoord: 7..521
e-value: 0.0
score: 621.632
IPR030667NEDD8-activating enzyme E1 regulatory subunit APP-BP1PIRSFPIRSF039099APP-BP1coord: 1..523
e-value: 4.9E-175
score: 581.3
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 7..519

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg03132Carg03132gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03132-RA:1Carg03132-RA:1exon
Carg03132-RA:2Carg03132-RA:2exon
Carg03132-RA:3Carg03132-RA:3exon
Carg03132-RA:4Carg03132-RA:4exon
Carg03132-RA:5Carg03132-RA:5exon
Carg03132-RA:6Carg03132-RA:6exon
Carg03132-RA:7Carg03132-RA:7exon
Carg03132-RA:8Carg03132-RA:8exon
Carg03132-RA:9Carg03132-RA:9exon
Carg03132-RA:10Carg03132-RA:10exon
Carg03132-RA:11Carg03132-RA:11exon
Carg03132-RA:12Carg03132-RA:12exon
Carg03132-RA:13Carg03132-RA:13exon
Carg03132-RA:14Carg03132-RA:14exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03132-RA:five_prime_utrCarg03132-RA:five_prime_utrfive_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03132-RA:cdsCarg03132-RA:cdsCDS
Carg03132-RA:cdsCarg03132-RA:cds_2CDS
Carg03132-RA:cdsCarg03132-RA:cds_3CDS
Carg03132-RA:cdsCarg03132-RA:cds_4CDS
Carg03132-RA:cdsCarg03132-RA:cds_5CDS
Carg03132-RA:cdsCarg03132-RA:cds_6CDS
Carg03132-RA:cdsCarg03132-RA:cds_7CDS
Carg03132-RA:cdsCarg03132-RA:cds_8CDS
Carg03132-RA:cdsCarg03132-RA:cds_9CDS
Carg03132-RA:cdsCarg03132-RA:cds_10CDS
Carg03132-RA:cdsCarg03132-RA:cds_11CDS
Carg03132-RA:cdsCarg03132-RA:cds_12CDS
Carg03132-RA:cdsCarg03132-RA:cds_13CDS
Carg03132-RA:cdsCarg03132-RA:cds_14CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03132-RA:three_prime_utrCarg03132-RA:three_prime_utrthree_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg03132-RACarg03132-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045116 protein neddylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0019781 NEDD8 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity