Carg02227-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg02227-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	BAG family molecular chaperone regulator 8, chloroplastic-like
Location	Carg_Chr15: 1359443 .. 1360864 (+)
Sequence length	1335
RNA-Seq Expression	Carg02227-RA
Synteny	Carg02227-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCTCATCACTATCCTCATCAAACCCATCCTTCCCCCGCCTGTTGCTGCGGCGGCGATTTCACCACTCCCTGCTTCCAAACCCACCACCACTCTCCTCCGCTGCCACCGTCTCACTCCGACCCCCTTCTTCAAGCTCTTGCTTCTCAAATTCTTCAATCCGCCCAGTTTCAGCAGTTTTCTCAGTACCCAAGTCTTCGTACCCAGAAATTTCAATCGCATTACCAATTTCAACCTCAAAATCAGAAGCGCTTTCGTGAGAAAGAACTTCCCCAAGTCGTCCACCATCCTACCATCTCCTCTCTTCTCTCCCGCATCGAAGCCCTTGAATCCTCTCTCCATCGAGTTTCTGGTTCTCATGTTTCTTTGCAATCTCTCCGGCAAGTTGCCGCGAGTACAATCCAGACACATTTTCGAGCGTTCCTTGTCCGCAGATCGCGAGCACTTAGAGAGCTCAAAGATCTCGCCATCATCAAGTCTAGATTTGAATCTCTCCAATCGTCGCTGTCCACTGAACTCTACTTTGATCGTAACGCCATTTCTCTCGAAATAATGGATTTGCTTCTCCATCTGGACTCCATTCAGGTAATTGTCGTTTGGTGGATGTATTTCATTTCTTCTACTTTCATTATAATTGATTCTAATTGATCTTCTCCATGATCTTCTTCTCAGGGCAGTGATCCAATGGTCAAAGATAGCAAGAAGTGCATTATCAGAGATTTGGTTCAGTTTTGGGAACGGATTGATTCCTTTGCTGTGAAGAGGAACAGACTTCCACTTAAGGCAATGAGAAATGCGAGGTTAGTTCAAAATGTCAATAAATCTATTGTTTCTAATCCTAAGCTTAAAAGCTATGAGAATCATAAAGAGACTATTGAGGAATTAAGGAACAGAGTTGAAAAGATTTGTGAGCAGATTTTTGAGAATGATGGGAAAGAACTGAATCTTAAAAGGTTTCACCATGTTGGTGACGTTGATGAGGGGAAGGAGGAGGAGTCCCCTAGAACAATTATCAAAGATAACAATCAAACTCAAAATGGGAAGCTTGCAAAAGGGCAACATATTGGAACTAGAGTGAAGAAAAGTGTTGGATTTGCTGAGAATGGAAATATCTGTAGGGTTTTAGGCAGCAACAGTCCACATGAAGAAGATTATGGTGTTTTGGATGAAGAAAGGGATTCAAGTGATGAGCTTGTGGAGATTAAGGAGTTTTCTGAGGTAGCTGAGGAAGATGGTGATAATGAAGAAGCACATGGAGGAGATGGTGGATCTCCAGAGGTCAGTGATGGAGAAAGAAACCGTCGAAGGACGATGACCAGTGGCCATGGAGGTGTTGCATTCTCTGCTCCCTTGCCATTGAAGATGGAGAATAAAGCGGAGGTTATGAAGAACAGAAAGTGTGTGAAAATTCTGGGATGA

mRNA sequence

ATGGCCTCTCATCACTATCCTCATCAAACCCATCCTTCCCCCGCCTGTTGCTGCGGCGGCGATTTCACCACTCCCTGCTTCCAAACCCACCACCACTCTCCTCCGCTGCCACCGTCTCACTCCGACCCCCTTCTTCAAGCTCTTGCTTCTCAAATTCTTCAATCCGCCCAGTTTCAGCAGTTTTCTCAGTACCCAAGTCTTCGTACCCAGAAATTTCAATCGCATTACCAATTTCAACCTCAAAATCAGAAGCGCTTTCGTGAGAAAGAACTTCCCCAAGTCGTCCACCATCCTACCATCTCCTCTCTTCTCTCCCGCATCGAAGCCCTTGAATCCTCTCTCCATCGAGTTTCTGGTTCTCATGTTTCTTTGCAATCTCTCCGGCAAGTTGCCGCGAGTACAATCCAGACACATTTTCGAGCGTTCCTTGTCCGCAGATCGCGAGCACTTAGAGAGCTCAAAGATCTCGCCATCATCAAGTCTAGATTTGAATCTCTCCAATCGTCGCTGTCCACTGAACTCTACTTTGATCGTAACGCCATTTCTCTCGAAATAATGGATTTGCTTCTCCATCTGGACTCCATTCAGGGCAGTGATCCAATGGTCAAAGATAGCAAGAAGTGCATTATCAGAGATTTGGTTCAGTTTTGGGAACGGATTGATTCCTTTGCTGTGAAGAGGAACAGACTTCCACTTAAGGCAATGAGAAATGCGAGGTTAGTTCAAAATGTCAATAAATCTATTGTTTCTAATCCTAAGCTTAAAAGCTATGAGAATCATAAAGAGACTATTGAGGAATTAAGGAACAGAGTTGAAAAGATTTGTGAGCAGATTTTTGAGAATGATGGGAAAGAACTGAATCTTAAAAGGTTTCACCATGTTGGTGACGTTGATGAGGGGAAGGAGGAGGAGTCCCCTAGAACAATTATCAAAGATAACAATCAAACTCAAAATGGGAAGCTTGCAAAAGGGCAACATATTGGAACTAGAGTGAAGAAAAGTGTTGGATTTGCTGAGAATGGAAATATCTGTAGGGTTTTAGGCAGCAACAGTCCACATGAAGAAGATTATGGTGTTTTGGATGAAGAAAGGGATTCAAGTGATGAGCTTGTGGAGATTAAGGAGTTTTCTGAGGTAGCTGAGGAAGATGGTGATAATGAAGAAGCACATGGAGGAGATGGTGGATCTCCAGAGGTCAGTGATGGAGAAAGAAACCGTCGAAGGACGATGACCAGTGGCCATGGAGGTGTTGCATTCTCTGCTCCCTTGCCATTGAAGATGGAGAATAAAGCGGAGGTTATGAAGAACAGAAAGTGTGTGAAAATTCTGGGATGA

Coding sequence (CDS)

ATGGCCTCTCATCACTATCCTCATCAAACCCATCCTTCCCCCGCCTGTTGCTGCGGCGGCGATTTCACCACTCCCTGCTTCCAAACCCACCACCACTCTCCTCCGCTGCCACCGTCTCACTCCGACCCCCTTCTTCAAGCTCTTGCTTCTCAAATTCTTCAATCCGCCCAGTTTCAGCAGTTTTCTCAGTACCCAAGTCTTCGTACCCAGAAATTTCAATCGCATTACCAATTTCAACCTCAAAATCAGAAGCGCTTTCGTGAGAAAGAACTTCCCCAAGTCGTCCACCATCCTACCATCTCCTCTCTTCTCTCCCGCATCGAAGCCCTTGAATCCTCTCTCCATCGAGTTTCTGGTTCTCATGTTTCTTTGCAATCTCTCCGGCAAGTTGCCGCGAGTACAATCCAGACACATTTTCGAGCGTTCCTTGTCCGCAGATCGCGAGCACTTAGAGAGCTCAAAGATCTCGCCATCATCAAGTCTAGATTTGAATCTCTCCAATCGTCGCTGTCCACTGAACTCTACTTTGATCGTAACGCCATTTCTCTCGAAATAATGGATTTGCTTCTCCATCTGGACTCCATTCAGGGCAGTGATCCAATGGTCAAAGATAGCAAGAAGTGCATTATCAGAGATTTGGTTCAGTTTTGGGAACGGATTGATTCCTTTGCTGTGAAGAGGAACAGACTTCCACTTAAGGCAATGAGAAATGCGAGGTTAGTTCAAAATGTCAATAAATCTATTGTTTCTAATCCTAAGCTTAAAAGCTATGAGAATCATAAAGAGACTATTGAGGAATTAAGGAACAGAGTTGAAAAGATTTGTGAGCAGATTTTTGAGAATGATGGGAAAGAACTGAATCTTAAAAGGTTTCACCATGTTGGTGACGTTGATGAGGGGAAGGAGGAGGAGTCCCCTAGAACAATTATCAAAGATAACAATCAAACTCAAAATGGGAAGCTTGCAAAAGGGCAACATATTGGAACTAGAGTGAAGAAAAGTGTTGGATTTGCTGAGAATGGAAATATCTGTAGGGTTTTAGGCAGCAACAGTCCACATGAAGAAGATTATGGTGTTTTGGATGAAGAAAGGGATTCAAGTGATGAGCTTGTGGAGATTAAGGAGTTTTCTGAGGTAGCTGAGGAAGATGGTGATAATGAAGAAGCACATGGAGGAGATGGTGGATCTCCAGAGGTCAGTGATGGAGAAAGAAACCGTCGAAGGACGATGACCAGTGGCCATGGAGGTGTTGCATTCTCTGCTCCCTTGCCATTGAAGATGGAGAATAAAGCGGAGGTTATGAAGAACAGAAAGTGTGTGAAAATTCTGGGATGA

Protein sequence

MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQFSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGSHVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNAISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARLVQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEGKEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYGVLDEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFSAPLPLKMENKAEVMKNRKCVKILG

Homology

[Close]

BLAST of Carg02227-RA vs. NCBI nr
Match: KAG7016115.1 (BAG family molecular chaperone regulator 8, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 861.7 bits (2225), Expect = 2.8e-246
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60
           MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ
Sbjct: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60

Query: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120
           FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS
Sbjct: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120

Query: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180
           HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA
Sbjct: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180

Query: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240
           ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL
Sbjct: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240

Query: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300
           VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG
Sbjct: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300

Query: 301 KEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360
           KEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYGVL
Sbjct: 301 KEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360

Query: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420
           DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS
Sbjct: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420

Query: 421 APLPLKMENKAEVMKNRKCVKILG 445
           APLPLKMENKAEVMKNRKCVKILG
Sbjct: 421 APLPLKMENKAEVMKNRKCVKILG 444

[Close]

BLAST of Carg02227-RA vs. NCBI nr
Match: KAG6578555.1 (BAG family molecular chaperone regulator 8, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 850.9 bits (2197), Expect = 5.0e-243
Identity = 439/444 (98.87%), Postives = 442/444 (99.55%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60
           MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPS SDPLLQALASQILQSAQFQQ
Sbjct: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSPSDPLLQALASQILQSAQFQQ 60

Query: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120
           FSQYPSLRTQKFQSHYQFQPQNQKRFRE+ELPQVVHHPTISSLLSRIEALESSLHRVSGS
Sbjct: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREQELPQVVHHPTISSLLSRIEALESSLHRVSGS 120

Query: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180
           HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA
Sbjct: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180

Query: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240
           ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIR+LVQFWERIDSFAVKRNRLPLKAMRNARL
Sbjct: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRNLVQFWERIDSFAVKRNRLPLKAMRNARL 240

Query: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300
           VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG
Sbjct: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300

Query: 301 KEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360
           +EEESPRTIIKDNNQTQNGKLAKGQHIG RVKKSVGFAENGNICRVLGSNSPHEEDYGVL
Sbjct: 301 EEEESPRTIIKDNNQTQNGKLAKGQHIGARVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360

Query: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420
           DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS
Sbjct: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420

Query: 421 APLPLKMENKAEVMKNRKCVKILG 445
           APLPLKMENKAEVMKNRKCVKILG
Sbjct: 421 APLPLKMENKAEVMKNRKCVKILG 444

[Close]

BLAST of Carg02227-RA vs. NCBI nr
Match: XP_022938985.1 (BAG family molecular chaperone regulator 8, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 848.2 bits (2190), Expect = 3.2e-242
Identity = 439/444 (98.87%), Postives = 440/444 (99.10%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60
           MASHHYPHQTH SPACCCGGDFTTPCFQTHHHSPPLPPS SDPLLQALASQILQSAQFQQ
Sbjct: 1   MASHHYPHQTHLSPACCCGGDFTTPCFQTHHHSPPLPPSPSDPLLQALASQILQSAQFQQ 60

Query: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120
           FSQYPSLRTQKFQSHYQFQPQNQKRFRE+ELPQVVHHPTISSLLSRIEALESSLHRVSGS
Sbjct: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREQELPQVVHHPTISSLLSRIEALESSLHRVSGS 120

Query: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180
           HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA
Sbjct: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180

Query: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240
           ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQ WERIDSFAVKRNRLPLKAMRNARL
Sbjct: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQLWERIDSFAVKRNRLPLKAMRNARL 240

Query: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300
           VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG
Sbjct: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300

Query: 301 KEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360
           KEEESPRTIIKDNNQTQNGKLAKGQHIG RVKKSVGFAENGNICRVLGSNSPHEEDYGVL
Sbjct: 301 KEEESPRTIIKDNNQTQNGKLAKGQHIGARVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360

Query: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420
           DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS
Sbjct: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420

Query: 421 APLPLKMENKAEVMKNRKCVKILG 445
           APLPLKMENKAEVMKNRKCVKILG
Sbjct: 421 APLPLKMENKAEVMKNRKCVKILG 444

[Close]

BLAST of Carg02227-RA vs. NCBI nr
Match: XP_023551187.1 (BAG family molecular chaperone regulator 8, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 797.7 bits (2059), Expect = 5.0e-227
Identity = 421/447 (94.18%), Postives = 427/447 (95.53%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60
           MASHHYPHQTH   ACCCGGDFTTPCFQ+ HHSPPLPPS SDPLLQALASQILQSAQFQ 
Sbjct: 1   MASHHYPHQTHLPHACCCGGDFTTPCFQSPHHSPPLPPSPSDPLLQALASQILQSAQFQH 60

Query: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120
           +SQYPS RTQKFQSHYQFQPQNQKRFRE+ELPQVVHHPTISSLLSRIEALESSLHRVSGS
Sbjct: 61  YSQYPSHRTQKFQSHYQFQPQNQKRFREQELPQVVHHPTISSLLSRIEALESSLHRVSGS 120

Query: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180
           HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLS E YFDRN 
Sbjct: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSNEFYFDRNT 180

Query: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240
           ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL
Sbjct: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240

Query: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICE--QIFENDGKELNLKRFHHVGDVD 300
           VQNVNKSIVSNPKLKSYEN KETIEEL +RVEKICE  +IFENDGKELNLKRF HVGDVD
Sbjct: 241 VQNVNKSIVSNPKLKSYENQKETIEELWSRVEKICERSRIFENDGKELNLKRFQHVGDVD 300

Query: 301 EGK-EEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDY 360
           EGK EEESPRTIIKDNNQT+NGKLAKGQHIG RVKKSVG AENGNICRVLGSNSPHEEDY
Sbjct: 301 EGKEEEESPRTIIKDNNQTRNGKLAKGQHIGVRVKKSVGLAENGNICRVLGSNSPHEEDY 360

Query: 361 GVLDEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGV 420
           GVLDEERDSSDELVEIKEFSEVAEEDGDNEEAHGGD G PEVSDGERNRRRTMTSGHGGV
Sbjct: 361 GVLDEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDDGFPEVSDGERNRRRTMTSGHGGV 420

Query: 421 AFSAPLPLKMENKAEVMKNRKCVKILG 445
           AFSAPLPLKMENKAEVMKNRKCVKILG
Sbjct: 421 AFSAPLPLKMENKAEVMKNRKCVKILG 447

[Close]

BLAST of Carg02227-RA vs. NCBI nr
Match: XP_022993612.1 (LOW QUALITY PROTEIN: BAG family molecular chaperone regulator 8, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 770.8 bits (1989), Expect = 6.5e-219
Identity = 406/439 (92.48%), Postives = 414/439 (94.31%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60
           MASHHYPHQTH  PACCCGGDFTTPCFQTHHHSPPLPPS SDPLLQALASQILQSAQFQ 
Sbjct: 1   MASHHYPHQTHLPPACCCGGDFTTPCFQTHHHSPPLPPSPSDPLLQALASQILQSAQFQH 60

Query: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120
           +SQY S RTQKFQSHYQFQPQNQKRFRE+ELPQVVHHPTISSLLSRIEALESSLHRVSGS
Sbjct: 61  YSQYSSFRTQKFQSHYQFQPQNQKRFREQELPQVVHHPTISSLLSRIEALESSLHRVSGS 120

Query: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180
           HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTEL FDRNA
Sbjct: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELNFDRNA 180

Query: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240
           I LEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL
Sbjct: 181 IYLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240

Query: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICE--QIFENDGKELNLKRFHHVGDVD 300
           VQNVNKSIVSNPKLKSYEN KETIEEL +RVEKICE  +IFENDGKELNLKRF H GDVD
Sbjct: 241 VQNVNKSIVSNPKLKSYENQKETIEELWSRVEKICERSRIFENDGKELNLKRFQHAGDVD 300

Query: 301 EGKEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYG 360
           EGK EES RT I DN+QT+NGKLAKGQHIG RVKKSVGF+ENGNIC+VLGSNS HEEDYG
Sbjct: 301 EGKGEESSRTFIXDNDQTRNGKLAKGQHIGARVKKSVGFSENGNICKVLGSNSQHEEDYG 360

Query: 361 VLDEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVA 420
           V DEERDSSDELVEIKEFSEVAEED DNEEAHG D GSPEVSDGERNRRR MTSGHGGVA
Sbjct: 361 VFDEERDSSDELVEIKEFSEVAEEDDDNEEAHGEDDGSPEVSDGERNRRRMMTSGHGGVA 420

Query: 421 FSAPLPLKMENKAEVMKNR 438
           FSAPLPLKMENKAEVMKNR
Sbjct: 421 FSAPLPLKMENKAEVMKNR 439

[Close]

BLAST of Carg02227-RA vs. ExPASy Swiss-Prot
Match: Q9LIB3 (BAG family molecular chaperone regulator 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAG1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.8e-23
Identity = 134/469 (28.57%), Postives = 209/469 (44.56%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDF--TTPCFQT-----HHHSPPLPPSHSDPLLQALASQIL 60
           MASHH+ +  H    C    +    TP F T     +  + P PP   D LL  +A+ + 
Sbjct: 1   MASHHHHNHNH---VCSRHQNHHNNTPQFATSPNCCNKSNHPSPPPAEDNLLHLVATYLQ 60

Query: 61  QSAQFQQFSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESS 120
              Q  Q S   S      Q+    + QN+   + K +P+      +S LL +I+ LESS
Sbjct: 61  NHQQETQCSCETSC-----QNFNVIRSQNRVLRQHKNVPREYDQVVLSCLLRKIDDLESS 120

Query: 121 LHRVSGSHVSLQ----SLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSS 180
           L++ S  +   +    +LR  AA  IQTHFR++LV RS + R+LK+LA+IK+ F SL+SS
Sbjct: 121 LNKFSSFYDKRRDRHSTLRDSAARVIQTHFRSYLVHRSISFRQLKELAMIKASFLSLKSS 180

Query: 181 LSTELYFDRNAISLEIMDLLLHLDSIQGS-DPMVKDSKKCIIRDLVQFWERIDSFAVKRN 240
           +S +L F    +S +  DLLL LDSIQG  DPM++ SK+ + RDLV+F + +D   VKR 
Sbjct: 181 VSGKLIFPFKVVSRKATDLLLQLDSIQGRIDPMIRSSKRSLSRDLVRFVQYVDDCVVKRY 240

Query: 241 RLPLKA----MRNARLVQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQ------I 300
              +K+      N +  Q    S           +  E +        K+         +
Sbjct: 241 GFVVKSGSGIKLNGKKPQGFGTSSEDEDNNADMSDDSEEVPVSSIDKRKVASSKSRTGVV 300

Query: 301 FENDGKELNLKRF----------------HHV---GDVDEGKEEESPRTIIKDNNQTQNG 360
            E D  +  + +F                H +    + D    +E    + +DN + Q+ 
Sbjct: 301 IEGDVVKPPVMKFVVLDKNRNVCQVYGNRHDLTSSAEDDSVDGDEETLVMSRDNGRKQSL 360

Query: 361 KLAKGQHI------GTRVKKSVGFAENGNICRVLGSNSPHEEDYGVLDEERDSSDELVEI 420
           K   G  +       TRV K+V F ENGN+C+V G       D   L    +  D +   
Sbjct: 361 KARNGVSVKGGGGKTTRVVKTVSFDENGNVCKVYG-------DTHDLTSSAEDDDSVDVG 420

Query: 421 KEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNR-RRTMTSGHGGVAFSA 422
           +E   +  ++G    +  G     + S G+ NR  +T++    G  + A
Sbjct: 421 EETLVMCRDEGKRRSSKTGSRVLVKGSGGKTNRVVKTVSFDENGNVYKA 454

[Close]

BLAST of Carg02227-RA vs. ExPASy Swiss-Prot
Match: Q9LVA0 (BAG family molecular chaperone regulator 7 OS=Arabidopsis thaliana OX=3702 GN=BAG7 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 9.7e-08
Identity = 36/101 (35.64%), Postives = 62/101 (61.39%), Query Frame = 0


Query: 129 QVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELY--------FDRNA 188
           + AA  IQ  F+A+L+RRS++LR L+DLAI K++ + L++S     Y         +R  
Sbjct: 304 EYAAVMIQRAFKAYLIRRSKSLRALRDLAIAKTKLKELRASFHNFSYRRLIARDGEERQK 363

Query: 189 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERID 222
            S +I+ LLL +D+I+G D MV+ +K+ ++ +L    + +D
Sbjct: 364 FSEKIIVLLLTVDAIEGVDVMVRGAKRSMVDELEAMLDVVD 404

[Close]

BLAST of Carg02227-RA vs. ExPASy Swiss-Prot
Match: O82345 (BAG family molecular chaperone regulator 6 OS=Arabidopsis thaliana OX=3702 GN=BAG6 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-04
Identity = 29/100 (29.00%), Postives = 58/100 (58.00%), Query Frame = 0


Query: 131 AASTIQTHFRAFLVRRSRALRELKDLAI-------IKSRFESLQSSLSTELYFDRNAISL 190
           AA  IQ+ +R + VRR   +++LK++A        +K R E+L++S    +      ++ 
Sbjct: 571 AARIIQSMYRGYDVRRWEPIKKLKEIATVREQMGDVKKRIEALEASTDQHIEEKEIVVNG 630

Query: 191 E-IMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDS 223
           E +M+LLL LD+++G  P +++ +K +  +L    +++DS
Sbjct: 631 ELVMNLLLKLDAVEGLHPSIREFRKALATELSSIQDKLDS 670

[Close]

BLAST of Carg02227-RA vs. ExPASy TrEMBL
Match: A0A6J1FLD2 (BAG family molecular chaperone regulator 8, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445030 PE=4 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.6e-242
Identity = 439/444 (98.87%), Postives = 440/444 (99.10%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60
           MASHHYPHQTH SPACCCGGDFTTPCFQTHHHSPPLPPS SDPLLQALASQILQSAQFQQ
Sbjct: 1   MASHHYPHQTHLSPACCCGGDFTTPCFQTHHHSPPLPPSPSDPLLQALASQILQSAQFQQ 60

Query: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120
           FSQYPSLRTQKFQSHYQFQPQNQKRFRE+ELPQVVHHPTISSLLSRIEALESSLHRVSGS
Sbjct: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREQELPQVVHHPTISSLLSRIEALESSLHRVSGS 120

Query: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180
           HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA
Sbjct: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180

Query: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240
           ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQ WERIDSFAVKRNRLPLKAMRNARL
Sbjct: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQLWERIDSFAVKRNRLPLKAMRNARL 240

Query: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300
           VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG
Sbjct: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQIFENDGKELNLKRFHHVGDVDEG 300

Query: 301 KEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360
           KEEESPRTIIKDNNQTQNGKLAKGQHIG RVKKSVGFAENGNICRVLGSNSPHEEDYGVL
Sbjct: 301 KEEESPRTIIKDNNQTQNGKLAKGQHIGARVKKSVGFAENGNICRVLGSNSPHEEDYGVL 360

Query: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420
           DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS
Sbjct: 361 DEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVAFS 420

Query: 421 APLPLKMENKAEVMKNRKCVKILG 445
           APLPLKMENKAEVMKNRKCVKILG
Sbjct: 421 APLPLKMENKAEVMKNRKCVKILG 444

[Close]

BLAST of Carg02227-RA vs. ExPASy TrEMBL
Match: A0A6J1JTB4 (LOW QUALITY PROTEIN: BAG family molecular chaperone regulator 8, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489552 PE=4 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.2e-219
Identity = 406/439 (92.48%), Postives = 414/439 (94.31%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDFTTPCFQTHHHSPPLPPSHSDPLLQALASQILQSAQFQQ 60
           MASHHYPHQTH  PACCCGGDFTTPCFQTHHHSPPLPPS SDPLLQALASQILQSAQFQ 
Sbjct: 1   MASHHYPHQTHLPPACCCGGDFTTPCFQTHHHSPPLPPSPSDPLLQALASQILQSAQFQH 60

Query: 61  FSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESSLHRVSGS 120
           +SQY S RTQKFQSHYQFQPQNQKRFRE+ELPQVVHHPTISSLLSRIEALESSLHRVSGS
Sbjct: 61  YSQYSSFRTQKFQSHYQFQPQNQKRFREQELPQVVHHPTISSLLSRIEALESSLHRVSGS 120

Query: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELYFDRNA 180
           HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTEL FDRNA
Sbjct: 121 HVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELNFDRNA 180

Query: 181 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240
           I LEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL
Sbjct: 181 IYLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKRNRLPLKAMRNARL 240

Query: 241 VQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICE--QIFENDGKELNLKRFHHVGDVD 300
           VQNVNKSIVSNPKLKSYEN KETIEEL +RVEKICE  +IFENDGKELNLKRF H GDVD
Sbjct: 241 VQNVNKSIVSNPKLKSYENQKETIEELWSRVEKICERSRIFENDGKELNLKRFQHAGDVD 300

Query: 301 EGKEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGNICRVLGSNSPHEEDYG 360
           EGK EES RT I DN+QT+NGKLAKGQHIG RVKKSVGF+ENGNIC+VLGSNS HEEDYG
Sbjct: 301 EGKGEESSRTFIXDNDQTRNGKLAKGQHIGARVKKSVGFSENGNICKVLGSNSQHEEDYG 360

Query: 361 VLDEERDSSDELVEIKEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNRRRTMTSGHGGVA 420
           V DEERDSSDELVEIKEFSEVAEED DNEEAHG D GSPEVSDGERNRRR MTSGHGGVA
Sbjct: 361 VFDEERDSSDELVEIKEFSEVAEEDDDNEEAHGEDDGSPEVSDGERNRRRMMTSGHGGVA 420

Query: 421 FSAPLPLKMENKAEVMKNR 438
           FSAPLPLKMENKAEVMKNR
Sbjct: 421 FSAPLPLKMENKAEVMKNR 439

[Close]

BLAST of Carg02227-RA vs. ExPASy TrEMBL
Match: A0A6J1JUX2 (BAG family molecular chaperone regulator 8, chloroplastic-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111487725 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.9e-155
Identity = 335/473 (70.82%), Postives = 374/473 (79.07%), Query Frame = 0


Query: 1   MASHHYPHQ-------THPSPACCC---GGDFTTPCFQTHHHSPPLPPSHSDPLLQALAS 60
           MAS HYPHQ       TH   ACCC   GGDF  PCFQT H SPP PPS SDPLLQALAS
Sbjct: 1   MASRHYPHQPHLHLHRTHLPSACCCGCEGGDFANPCFQTLHPSPP-PPSPSDPLLQALAS 60

Query: 61  QILQSAQFQQFSQYPSLRTQKFQSHYQFQP---QNQKRFREKELPQVVHHPTISSLLSRI 120
           QILQSAQFQ  S YP+LRT+KFQSHYQFQP   QN KR RE+ELPQV+HH TISSL+SRI
Sbjct: 61  QILQSAQFQHLSHYPTLRTKKFQSHYQFQPLGSQNPKRVREQELPQVIHHSTISSLVSRI 120

Query: 121 EALESSLHRVSGSHVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQ 180
           EALESSLHRVSGSHV  QSLRQVAASTIQT+FRAFLVRRSRALRELKDLAIIKSRFESLQ
Sbjct: 121 EALESSLHRVSGSHVPPQSLRQVAASTIQTYFRAFLVRRSRALRELKDLAIIKSRFESLQ 180

Query: 181 SSLSTELYFDRNAISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKR 240
           SSL  E YFDRNAISLEIMDLLLHLDSIQG+DPMVK SKK + RDLVQF +RID+ +VKR
Sbjct: 181 SSLCNEFYFDRNAISLEIMDLLLHLDSIQGNDPMVKGSKKSLSRDLVQFLDRIDNLSVKR 240

Query: 241 N-RLPLKAMRNARLVQNVNKSIVSNPK-LKSYENHKETIEELRNRVEKICE--QIFENDG 300
           N  LPLK M+N  L QNV+KS VS PK LK+ EN K TIE+L++R+EKICE  + FENDG
Sbjct: 241 NGGLPLKTMKNTGLGQNVSKSRVSTPKQLKNSENQKGTIEKLKSRIEKICERSKFFENDG 300

Query: 301 KELNLKRFHHVG-DVDEGKEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGN 360
           ++L+LKRFHHVG D DE +E+ESPR   K+ +QT++  L  G  I  RVKKSV FAENG+
Sbjct: 301 RDLDLKRFHHVGDDDDEEEEDESPRIHDKNRDQTRDQNLGNGHQIQPRVKKSVRFAENGS 360

Query: 361 ICRVLGSNSPHEEDYGVLDE--ERDSSDEL--------VEIKEFSEVAEEDGDNEEAHGG 420
           + RVLGSNSPH EDYGVLDE  ERDS DEL        VEIKEFSEVAE+  D EEAHG 
Sbjct: 361 LFRVLGSNSPH-EDYGVLDERDERDSGDELVEDIFNEAVEIKEFSEVAED--DEEEAHG- 420

Query: 421 DGGSPEVSDGERNRRRTMTSGHGGVAFSAPLPLKMENKAE-VMKNRKCVKILG 445
                E+SDGERN  R M+SG+GG +FSAPLP+KMENKA+ +MKN K +KILG
Sbjct: 421 -----EMSDGERNPGRKMSSGNGGSSFSAPLPMKMENKADLIMKNSKSLKILG 463

[Close]

BLAST of Carg02227-RA vs. ExPASy TrEMBL
Match: A0A6J1JPI3 (BAG family molecular chaperone regulator 8, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487725 PE=4 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 1.4e-153
Identity = 331/471 (70.28%), Postives = 371/471 (78.77%), Query Frame = 0


Query: 1   MASHHYPHQ-------THPSPACCC---GGDFTTPCFQTHHHSPPLPPSHSDPLLQALAS 60
           MAS HYPHQ       TH   ACCC   GGDF  PCFQT H SPP PPS SDPLLQALAS
Sbjct: 1   MASRHYPHQPHLHLHRTHLPSACCCGCEGGDFANPCFQTLHPSPP-PPSPSDPLLQALAS 60

Query: 61  QILQSAQFQQFSQYPSLRTQKFQSHYQFQP---QNQKRFREKELPQVVHHPTISSLLSRI 120
           QILQSAQFQ  S YP+LRT+KFQSHYQFQP   QN KR RE+ELPQV+HH TISSL+SRI
Sbjct: 61  QILQSAQFQHLSHYPTLRTKKFQSHYQFQPLGSQNPKRVREQELPQVIHHSTISSLVSRI 120

Query: 121 EALESSLHRVSGSHVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQ 180
           EALESSLHRVSGSHV  QSLRQVAASTIQT+FRAFLVRRSRALRELKDLAIIKSRFESLQ
Sbjct: 121 EALESSLHRVSGSHVPPQSLRQVAASTIQTYFRAFLVRRSRALRELKDLAIIKSRFESLQ 180

Query: 181 SSLSTELYFDRNAISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKR 240
           SSL  E YFDRNAISLEIMDLLLHLDSIQG+DPMVK SKK + RDLVQF +RID+ +VKR
Sbjct: 181 SSLCNEFYFDRNAISLEIMDLLLHLDSIQGNDPMVKGSKKSLSRDLVQFLDRIDNLSVKR 240

Query: 241 N-RLPLKAMRNARLVQNVNKSIVSNPK-LKSYENHKETIEELRNRVEKICE--QIFENDG 300
           N  LPLK M+N  L QNV+KS VS PK LK+ EN K TIE+L++R+EKICE  + FENDG
Sbjct: 241 NGGLPLKTMKNTGLGQNVSKSRVSTPKQLKNSENQKGTIEKLKSRIEKICERSKFFENDG 300

Query: 301 KELNLKRFHHVG-DVDEGKEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGN 360
           ++L+LKRFHHVG D DE +E+ESPR   K+ +QT++  L  G  I  RVKKSV FAENG+
Sbjct: 301 RDLDLKRFHHVGDDDDEEEEDESPRIHDKNRDQTRDQNLGNGHQIQPRVKKSVRFAENGS 360

Query: 361 ICRVLGSNSPHEEDYGVLDE--ERDSSDEL--------VEIKEFSEVAEEDGDNEEAHGG 420
           + RVLGSNSPH EDYGVLDE  ERDS DEL        VEIKEFSEVAE+  D EEAHG 
Sbjct: 361 LFRVLGSNSPH-EDYGVLDERDERDSGDELVEDIFNEAVEIKEFSEVAED--DEEEAHG- 420

Query: 421 DGGSPEVSDGERNRRRTMTSGHGGVAFSAPLPLKMENKAE-VMKNRKCVKI 443
                E+SDGERN  R M+SG+GG +FSAPLP+KMENKA+ +MKNR  + +
Sbjct: 421 -----EMSDGERNPGRKMSSGNGGSSFSAPLPMKMENKADLIMKNRDVLNV 461

[Close]

BLAST of Carg02227-RA vs. ExPASy TrEMBL
Match: A0A6J1JKG7 (BAG family molecular chaperone regulator 8, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487725 PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 8.8e-153
Identity = 330/465 (70.97%), Postives = 368/465 (79.14%), Query Frame = 0


Query: 1   MASHHYPHQ-------THPSPACCC---GGDFTTPCFQTHHHSPPLPPSHSDPLLQALAS 60
           MAS HYPHQ       TH   ACCC   GGDF  PCFQT H SPP PPS SDPLLQALAS
Sbjct: 1   MASRHYPHQPHLHLHRTHLPSACCCGCEGGDFANPCFQTLHPSPP-PPSPSDPLLQALAS 60

Query: 61  QILQSAQFQQFSQYPSLRTQKFQSHYQFQP---QNQKRFREKELPQVVHHPTISSLLSRI 120
           QILQSAQFQ  S YP+LRT+KFQSHYQFQP   QN KR RE+ELPQV+HH TISSL+SRI
Sbjct: 61  QILQSAQFQHLSHYPTLRTKKFQSHYQFQPLGSQNPKRVREQELPQVIHHSTISSLVSRI 120

Query: 121 EALESSLHRVSGSHVSLQSLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQ 180
           EALESSLHRVSGSHV  QSLRQVAASTIQT+FRAFLVRRSRALRELKDLAIIKSRFESLQ
Sbjct: 121 EALESSLHRVSGSHVPPQSLRQVAASTIQTYFRAFLVRRSRALRELKDLAIIKSRFESLQ 180

Query: 181 SSLSTELYFDRNAISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDSFAVKR 240
           SSL  E YFDRNAISLEIMDLLLHLDSIQG+DPMVK SKK + RDLVQF +RID+ +VKR
Sbjct: 181 SSLCNEFYFDRNAISLEIMDLLLHLDSIQGNDPMVKGSKKSLSRDLVQFLDRIDNLSVKR 240

Query: 241 N-RLPLKAMRNARLVQNVNKSIVSNPK-LKSYENHKETIEELRNRVEKICE--QIFENDG 300
           N  LPLK M+N  L QNV+KS VS PK LK+ EN K TIE+L++R+EKICE  + FENDG
Sbjct: 241 NGGLPLKTMKNTGLGQNVSKSRVSTPKQLKNSENQKGTIEKLKSRIEKICERSKFFENDG 300

Query: 301 KELNLKRFHHVG-DVDEGKEEESPRTIIKDNNQTQNGKLAKGQHIGTRVKKSVGFAENGN 360
           ++L+LKRFHHVG D DE +E+ESPR   K+ +QT++  L  G  I  RVKKSV FAENG+
Sbjct: 301 RDLDLKRFHHVGDDDDEEEEDESPRIHDKNRDQTRDQNLGNGHQIQPRVKKSVRFAENGS 360

Query: 361 ICRVLGSNSPHEEDYGVLDE--ERDSSDEL--------VEIKEFSEVAEEDGDNEEAHGG 420
           + RVLGSNSPH EDYGVLDE  ERDS DEL        VEIKEFSEVAE+  D EEAHG 
Sbjct: 361 LFRVLGSNSPH-EDYGVLDERDERDSGDELVEDIFNEAVEIKEFSEVAED--DEEEAHG- 420

Query: 421 DGGSPEVSDGERNRRRTMTSGHGGVAFSAPLPLKMENKAE-VMKN 437
                E+SDGERN  R M+SG+GG +FSAPLP+KMENKA+ +MKN
Sbjct: 421 -----EMSDGERNPGRKMSSGNGGSSFSAPLPMKMENKADLIMKN 455

[Close]

BLAST of Carg02227-RA vs. TAIR 10
Match: AT3G29310.1 (calmodulin-binding protein-related )

HSP 1 Score: 111.3 bits (277), Expect = 2.0e-24
Identity = 134/469 (28.57%), Postives = 209/469 (44.56%), Query Frame = 0


Query: 1   MASHHYPHQTHPSPACCCGGDF--TTPCFQT-----HHHSPPLPPSHSDPLLQALASQIL 60
           MASHH+ +  H    C    +    TP F T     +  + P PP   D LL  +A+ + 
Sbjct: 1   MASHHHHNHNH---VCSRHQNHHNNTPQFATSPNCCNKSNHPSPPPAEDNLLHLVATYLQ 60

Query: 61  QSAQFQQFSQYPSLRTQKFQSHYQFQPQNQKRFREKELPQVVHHPTISSLLSRIEALESS 120
              Q  Q S   S      Q+    + QN+   + K +P+      +S LL +I+ LESS
Sbjct: 61  NHQQETQCSCETSC-----QNFNVIRSQNRVLRQHKNVPREYDQVVLSCLLRKIDDLESS 120

Query: 121 LHRVSGSHVSLQ----SLRQVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSS 180
           L++ S  +   +    +LR  AA  IQTHFR++LV RS + R+LK+LA+IK+ F SL+SS
Sbjct: 121 LNKFSSFYDKRRDRHSTLRDSAARVIQTHFRSYLVHRSISFRQLKELAMIKASFLSLKSS 180

Query: 181 LSTELYFDRNAISLEIMDLLLHLDSIQGS-DPMVKDSKKCIIRDLVQFWERIDSFAVKRN 240
           +S +L F    +S +  DLLL LDSIQG  DPM++ SK+ + RDLV+F + +D   VKR 
Sbjct: 181 VSGKLIFPFKVVSRKATDLLLQLDSIQGRIDPMIRSSKRSLSRDLVRFVQYVDDCVVKRY 240

Query: 241 RLPLKA----MRNARLVQNVNKSIVSNPKLKSYENHKETIEELRNRVEKICEQ------I 300
              +K+      N +  Q    S           +  E +        K+         +
Sbjct: 241 GFVVKSGSGIKLNGKKPQGFGTSSEDEDNNADMSDDSEEVPVSSIDKRKVASSKSRTGVV 300

Query: 301 FENDGKELNLKRF----------------HHV---GDVDEGKEEESPRTIIKDNNQTQNG 360
            E D  +  + +F                H +    + D    +E    + +DN + Q+ 
Sbjct: 301 IEGDVVKPPVMKFVVLDKNRNVCQVYGNRHDLTSSAEDDSVDGDEETLVMSRDNGRKQSL 360

Query: 361 KLAKGQHI------GTRVKKSVGFAENGNICRVLGSNSPHEEDYGVLDEERDSSDELVEI 420
           K   G  +       TRV K+V F ENGN+C+V G       D   L    +  D +   
Sbjct: 361 KARNGVSVKGGGGKTTRVVKTVSFDENGNVCKVYG-------DTHDLTSSAEDDDSVDVG 420

Query: 421 KEFSEVAEEDGDNEEAHGGDGGSPEVSDGERNR-RRTMTSGHGGVAFSA 422
           +E   +  ++G    +  G     + S G+ NR  +T++    G  + A
Sbjct: 421 EETLVMCRDEGKRRSSKTGSRVLVKGSGGKTNRVVKTVSFDENGNVYKA 454

[Close]

BLAST of Carg02227-RA vs. TAIR 10
Match: AT5G62390.1 (BCL-2-associated athanogene 7 )

HSP 1 Score: 59.7 bits (143), Expect = 6.9e-09
Identity = 36/101 (35.64%), Postives = 62/101 (61.39%), Query Frame = 0


Query: 129 QVAASTIQTHFRAFLVRRSRALRELKDLAIIKSRFESLQSSLSTELY--------FDRNA 188
           + AA  IQ  F+A+L+RRS++LR L+DLAI K++ + L++S     Y         +R  
Sbjct: 304 EYAAVMIQRAFKAYLIRRSKSLRALRDLAIAKTKLKELRASFHNFSYRRLIARDGEERQK 363

Query: 189 ISLEIMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERID 222
            S +I+ LLL +D+I+G D MV+ +K+ ++ +L    + +D
Sbjct: 364 FSEKIIVLLLTVDAIEGVDVMVRGAKRSMVDELEAMLDVVD 404

[Close]

BLAST of Carg02227-RA vs. TAIR 10
Match: AT2G46240.1 (BCL-2-associated athanogene 6 )

HSP 1 Score: 49.3 bits (116), Expect = 9.3e-06
Identity = 29/100 (29.00%), Postives = 58/100 (58.00%), Query Frame = 0


Query: 131 AASTIQTHFRAFLVRRSRALRELKDLAI-------IKSRFESLQSSLSTELYFDRNAISL 190
           AA  IQ+ +R + VRR   +++LK++A        +K R E+L++S    +      ++ 
Sbjct: 571 AARIIQSMYRGYDVRRWEPIKKLKEIATVREQMGDVKKRIEALEASTDQHIEEKEIVVNG 630

Query: 191 E-IMDLLLHLDSIQGSDPMVKDSKKCIIRDLVQFWERIDS 223
           E +M+LLL LD+++G  P +++ +K +  +L    +++DS
Sbjct: 631 ELVMNLLLKLDAVEGLHPSIREFRKALATELSSIQDKLDS 670

The following BLAST results are available for this feature:

BLAST of Carg02227-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016115.1	2.8e-246	100.00	BAG family molecular chaperone regulator 8, chloroplastic, partial [Cucurbita ar...	[more]
KAG6578555.1	5.0e-243	98.87	BAG family molecular chaperone regulator 8, chloroplastic, partial [Cucurbita ar...	[more]
XP_022938985.1	3.2e-242	98.87	BAG family molecular chaperone regulator 8, chloroplastic-like [Cucurbita moscha...	[more]
XP_023551187.1	5.0e-227	94.18	BAG family molecular chaperone regulator 8, chloroplastic-like [Cucurbita pepo s...	[more]
XP_022993612.1	6.5e-219	92.48	LOW QUALITY PROTEIN: BAG family molecular chaperone regulator 8, chloroplastic-l...	[more]

BLAST of Carg02227-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIB3	2.8e-23	28.57	BAG family molecular chaperone regulator 8, chloroplastic OS=Arabidopsis thalian...	[more]
Q9LVA0	9.7e-08	35.64	BAG family molecular chaperone regulator 7 OS=Arabidopsis thaliana OX=3702 GN=BA...	[more]
O82345	1.3e-04	29.00	BAG family molecular chaperone regulator 6 OS=Arabidopsis thaliana OX=3702 GN=BA...	[more]

BLAST of Carg02227-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FLD2	1.6e-242	98.87	BAG family molecular chaperone regulator 8, chloroplastic-like OS=Cucurbita mosc...	[more]
A0A6J1JTB4	3.2e-219	92.48	LOW QUALITY PROTEIN: BAG family molecular chaperone regulator 8, chloroplastic-l...	[more]
A0A6J1JUX2	1.9e-155	70.82	BAG family molecular chaperone regulator 8, chloroplastic-like isoform X3 OS=Cuc...	[more]
A0A6J1JPI3	1.4e-153	70.28	BAG family molecular chaperone regulator 8, chloroplastic-like isoform X1 OS=Cuc...	[more]
A0A6J1JKG7	8.8e-153	70.97	BAG family molecular chaperone regulator 8, chloroplastic-like isoform X2 OS=Cuc...	[more]

BLAST of Carg02227-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29310.1	2.0e-24	28.57	calmodulin-binding protein-related	[more]
AT5G62390.1	6.9e-09	35.64	BCL-2-associated athanogene 7	[more]
AT2G46240.1	9.3e-06	29.00	BCL-2-associated athanogene 6	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 254..277
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 389..410
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 347..419
None	No IPR available	PANTHER	PTHR33322:SF18	BAG FAMILY MOLECULAR CHAPERONE REGULATOR 8, CHLOROPLASTIC	coord: 1..434
IPR040400	BAG family molecular chaperone regulator 5/6/7/8	PANTHER	PTHR33322	BAG DOMAIN CONTAINING PROTEIN, EXPRESSED	coord: 1..434
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 128..157 score: 6.5953

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg02227	Carg02227	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg02227-RA:1	Carg02227-RA:1	exon
Carg02227-RA:2	Carg02227-RA:2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg02227-RA:cds	Carg02227-RA:cds	CDS
Carg02227-RA:cds	Carg02227-RA:cds_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg02227-RA	Carg02227-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0051087	chaperone binding