Carg02191-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02191-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProline dehydrogenase
LocationCarg_Chr15: 1166047 .. 1168952 (-)
Sequence length1386
RNA-Seq ExpressionCarg02191-RA
SyntenyCarg02191-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAGAAGTATCCTTTCAACCCAATCAAAGATCCCCAAGAACGTATTTTTTTACGCTTTCAATCGGTTGTTGAACACCGCCGCATCCTCCTCCGCCGCTGCCTTCACTTCCGCCGCTTCTACGCCGCCGCCGCATCTCAATCTCGCTGTCTCTCCGGCGGCACCCACCGTCGATTTTTCTGACACGAAGGCCTTGTTTGGGTCGTTATCAACGTTGGAGGTCCTACACGCCGCCGCCAGCCTCCATGTCGCCGCTGTCGGCCCTGTCGTAGACGTCGGAATGTGGGTTATGAACTCTAAATTGATGGATGTTGAGTTGGCTCGTGATGTAATTTTTGGTACCGTTAGACATAGTTTTTACCGACATTTTTGCGCCGGCGAGGACGACACGGCTGTCGGGAAAACCGTTCGCCGGTTGCATAATGTCGGGCTGAGAAGCATGCTCGATTACGCTATTGAATACGCCAACGATGAGGAGTCCTGTGATCGGAATCTTCATGGGTTTCTCCGAACCATTGAGGCCACTAGAGCGCTCCCACGTGGCTCTGTAAGTTCGTAAATTTTTTTTTGTTTAATACGGGAATAATATTGTCCACTTTGAGTATATGTTCATGATTTTGTTTTGGGTCTCTCCAAAATGTCTCAGACCACTTGTAAACGGCGATTCATTCTCTAAATTAGTCTACCTAGGACTCCCTCCCAACAATCTTCCGAGCCTTCCTTTGATGTCTATGTAGCCCTCGAACAGTTTCTCCTTAATCGAGGTTTAACTCTTGTCTCTGGAACCCTAGAACAAAATACACCCTTTGTTGACACAAGTTACTTTTGACTACACCTTCGATGCTAGTAATATCTTTGTTCTCATTCTCTAAATTAGTCCATATGGGACTCCCTCCCAACAATCTTCCGAGCCTTCCTTTAATGTCTATGTAGCCCTCGAACAGCTTCTCCTTAATCGAGGCTCAACTCTTGTCTCTAGAACCCGGGAACAAAGTACCCTTTTATTGACCCGAGTCACTTTTGCCTACACTTTTAAGGCTAGCAATTTCTTTATTCTTAAGGGCATGACTTTGACACCATGTTAGGAATCATACTCTCCACACGAGTATGATATTGTCTATTTTGAGTATAAGCTTCATGGCTTTGATTTGGGATTTTGGAAAGGGTCTCGTACCCGATAGAGATGTATTCCTTACTTATAAACACATGATCATTCTCTAAATTAACGTTCGATTTGTTTCGTGCGATTGATAGGCAAGCTTTGTGGTGGCTAAAGTGAGCGCAATTTGCCCATTGAGATTGCTTGAGAGAGTGAGTGACTTGTTAAGATGGCAACAAAAGGACCCATGTTTGAATCTTCCATGGAAGCTCAAAACATTCCCAATTTTCTCTGAATCAAGCCCTCTTTACCACACTCTTCAACAGCCCAATCCCTTAACAAACGAAGAACAAAAAAGCCTCCAAATAAGCCATCAAAGGCTAATGAAAATCTGCCAAAGTTGCGTGGATGCCAACGTTCCTTTAGCCATCGACGCCGAGCACACCAAAATTCAACCCGCCATTGATTACTTCACATACTCCGCCGCCATCATCCACAACAAAGACCATAACCCAATAGTGTATGGAACCCTCCAAGCCTATCTAAAAGACGCAAAGGATCGGCTTTTGTTGGTCACCAAAGAGGCCAACGAAATGAGAGTTCCTCTTGGGATTAAGCTCGTGAGAGGTGCTTATATGTCTAGTGAATCCAAGCTCGCTTCTTCTCTTGGATTCGAATCTCCCATTCACAATAGCATTCATGACACACATGCATGCTATAATAGTTGCGCGTCGTTCTTGCTCGACAACATCGCCACAGGCTCGAGCGGAGCCATTCTCGCTACCCACAACGTCGAATCAGGTATCCGAAATCGTATCTCATTTAATAACATAGGTACCTAGAACCATGTCTCGTTTCATAACATGGGTATCGAGAATCGTGTCTCGTTTCATAACATGGGTATCCTGTCTCGTTATGGGAACCCAAAATCGTGTCTCATTAAGAGTACTCAGAATCGTGTCTTGTTTAATAACATGGGTACCTAGAACCGTGTCTCGTTTCATAACATGGGTATCCAGAATCGTGTCTCGTTTAATAACATGGGAATCCAGAATCGTGTCTCTTTGAATAACATGAGTACCCAGAATCGTGTCTCATTTAATAACATGGGTACCCCCCAGAATCGTGTCTCGTTTTAATGAGTACCCAGAATCGTGTCTCTGTTTAATAACATGGGTACCCAAAATCATGTTTCGTGTAATAACCATGTTAGAACGTTGTTCATAATCTAGATCTCGTTCGATAACCATTTTATACCCACAATGTCGAATCCTGTAACACCCACATTAGAACGTTATTCATAACATGGGTCTCATTTGATAACCGTTCTACAGGAAAATTAGCTGCAAAAAAAGCGTACGAGATAGGGATCGAGAAGTTGAAGAAAAAGCTCGAGTTTGCTCAATTGTATGGAATGTCGGAAGCGTTGTCGTATGGGTTAAGAAACGCAGGGTTTCAAGTAAGCAAATACATGCCATTTGGAGCAGTGGATATGGTAATGCCATACCTTCTAAGAAGAGCAGAGGAGAATAGAGGCCTCTTATCGGCCTCAAATTTGGACAGACAGCTTATGAGGTATAGAAATATAAATATTTTGTAGGGAGGGACTTGATAGCAAATTTGACATCTTTTTTAATTTCTTGATATTTGCAGAAAGGAGCTCGGGAGGAGGGTGAAAGAGTACTTGAGGGGTGAAAGAAGGGCCTTTTAGAAAATTCTCCTTTCTACAAAATTTGCAATTTAGTCCCTTTCAGTCATTTTTGCCATTTAGCTTACCGCTCGCTATCGGTCTCCATCATTTATAA

mRNA sequence

ATGGCCAGAAGTATCCTTTCAACCCAATCAAAGATCCCCAAGAACGTATTTTTTTACGCTTTCAATCGGTTGTTGAACACCGCCGCATCCTCCTCCGCCGCTGCCTTCACTTCCGCCGCTTCTACGCCGCCGCCGCATCTCAATCTCGCTGTCTCTCCGGCGGCACCCACCGTCGATTTTTCTGACACGAAGGCCTTGTTTGGGTCGTTATCAACGTTGGAGGTCCTACACGCCGCCGCCAGCCTCCATGTCGCCGCTGTCGGCCCTGTCGTAGACGTCGGAATGTGGGTTATGAACTCTAAATTGATGGATGTTGAGTTGGCTCGTGATGTAATTTTTGGTACCGTTAGACATAGTTTTTACCGACATTTTTGCGCCGGCGAGGACGACACGGCTGTCGGGAAAACCGTTCGCCGGTTGCATAATGTCGGGCTGAGAAGCATGCTCGATTACGCTATTGAATACGCCAACGATGAGGAGTCCTGTGATCGGAATCTTCATGGATGGCAACAAAAGGACCCATGTTTGAATCTTCCATGGAAGCTCAAAACATTCCCAATTTTCTCTGAATCAAGCCCTCTTTACCACACTCTTCAACAGCCCAATCCCTTAACAAACGAAGAACAAAAAAGCCTCCAAATAAGCCATCAAAGGCTAATGAAAATCTGCCAAAGTTGCGTGGATGCCAACGTTCCTTTAGCCATCGACGCCGAGCACACCAAAATTCAACCCGCCATTGATTACTTCACATACTCCGCCGCCATCATCCACAACAAAGACCATAACCCAATAGTGTATGGAACCCTCCAAGCCTATCTAAAAGACGCAAAGGATCGGCTTTTGTTGGTCACCAAAGAGGCCAACGAAATGAGAGTTCCTCTTGGGATTAAGCTCGTGAGAGGTGCTTATATGTCTAGTGAATCCAAGCTCGCTTCTTCTCTTGGATTCGAATCTCCCATTCACAATAGCATTCATGACACACATGCATGCTATAATAGTTGCGCGTCGTTCTTGCTCGACAACATCGCCACAGGCTCGAGCGGAGCCATTCTCGCTACCCACAACGTCGAATCAGGAAAATTAGCTGCAAAAAAAGCGTACGAGATAGGGATCGAGAAGTTGAAGAAAAAGCTCGAGTTTGCTCAATTGTATGGAATGTCGGAAGCGTTGTCGTATGGGTTAAGAAACGCAGGGTTTCAAGTAAGCAAATACATGCCATTTGGAGCAGTGGATATGGTAATGCCATACCTTCTAAGAAGAGCAGAGGAGAATAGAGGCCTCTTATCGGCCTCAAATTTGGACAGACAGCTTATGAGAAAGGAGCTCGGGAGGAGGTCATTTTTGCCATTTAGCTTACCGCTCGCTATCGGTCTCCATCATTTATAA

Coding sequence (CDS)

ATGGCCAGAAGTATCCTTTCAACCCAATCAAAGATCCCCAAGAACGTATTTTTTTACGCTTTCAATCGGTTGTTGAACACCGCCGCATCCTCCTCCGCCGCTGCCTTCACTTCCGCCGCTTCTACGCCGCCGCCGCATCTCAATCTCGCTGTCTCTCCGGCGGCACCCACCGTCGATTTTTCTGACACGAAGGCCTTGTTTGGGTCGTTATCAACGTTGGAGGTCCTACACGCCGCCGCCAGCCTCCATGTCGCCGCTGTCGGCCCTGTCGTAGACGTCGGAATGTGGGTTATGAACTCTAAATTGATGGATGTTGAGTTGGCTCGTGATGTAATTTTTGGTACCGTTAGACATAGTTTTTACCGACATTTTTGCGCCGGCGAGGACGACACGGCTGTCGGGAAAACCGTTCGCCGGTTGCATAATGTCGGGCTGAGAAGCATGCTCGATTACGCTATTGAATACGCCAACGATGAGGAGTCCTGTGATCGGAATCTTCATGGATGGCAACAAAAGGACCCATGTTTGAATCTTCCATGGAAGCTCAAAACATTCCCAATTTTCTCTGAATCAAGCCCTCTTTACCACACTCTTCAACAGCCCAATCCCTTAACAAACGAAGAACAAAAAAGCCTCCAAATAAGCCATCAAAGGCTAATGAAAATCTGCCAAAGTTGCGTGGATGCCAACGTTCCTTTAGCCATCGACGCCGAGCACACCAAAATTCAACCCGCCATTGATTACTTCACATACTCCGCCGCCATCATCCACAACAAAGACCATAACCCAATAGTGTATGGAACCCTCCAAGCCTATCTAAAAGACGCAAAGGATCGGCTTTTGTTGGTCACCAAAGAGGCCAACGAAATGAGAGTTCCTCTTGGGATTAAGCTCGTGAGAGGTGCTTATATGTCTAGTGAATCCAAGCTCGCTTCTTCTCTTGGATTCGAATCTCCCATTCACAATAGCATTCATGACACACATGCATGCTATAATAGTTGCGCGTCGTTCTTGCTCGACAACATCGCCACAGGCTCGAGCGGAGCCATTCTCGCTACCCACAACGTCGAATCAGGAAAATTAGCTGCAAAAAAAGCGTACGAGATAGGGATCGAGAAGTTGAAGAAAAAGCTCGAGTTTGCTCAATTGTATGGAATGTCGGAAGCGTTGTCGTATGGGTTAAGAAACGCAGGGTTTCAAGTAAGCAAATACATGCCATTTGGAGCAGTGGATATGGTAATGCCATACCTTCTAAGAAGAGCAGAGGAGAATAGAGGCCTCTTATCGGCCTCAAATTTGGACAGACAGCTTATGAGAAAGGAGCTCGGGAGGAGGTCATTTTTGCCATTTAGCTTACCGCTCGCTATCGGTCTCCATCATTTATAA

Protein sequence

MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELGRRSFLPFSLPLAIGLHHL
Homology
BLAST of Carg02191-RA vs. NCBI nr
Match: KAG7016079.1 (Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 907.9 bits (2345), Expect = 3.5e-260
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGWQQKDPCLNLPW 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGWQQKDPCLNLPW
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGWQQKDPCLNLPW 180

Query: 181 KLKTFPIFSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHT 240
           KLKTFPIFSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHT
Sbjct: 181 KLKTFPIFSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHT 240

Query: 241 KIQPAIDYFTYSAAIIHNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVR 300
           KIQPAIDYFTYSAAIIHNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVR
Sbjct: 241 KIQPAIDYFTYSAAIIHNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVR 300

Query: 301 GAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGK 360
           GAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGK
Sbjct: 301 GAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGK 360

Query: 361 LAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRR 420
           LAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRR
Sbjct: 361 LAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRR 420

Query: 421 AEENRGLLSASNLDRQLMRKELGRRSFLPFSLPLAIGLHHL 462
           AEENRGLLSASNLDRQLMRKELGRRSFLPFSLPLAIGLHHL
Sbjct: 421 AEENRGLLSASNLDRQLMRKELGRRSFLPFSLPLAIGLHHL 461

BLAST of Carg02191-RA vs. NCBI nr
Match: XP_022939392.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 855.9 bits (2210), Expect = 1.6e-244
Identity = 445/485 (91.75%), Postives = 445/485 (91.75%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG            
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRVLP 180

Query: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240
                                       WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 446
           AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

BLAST of Carg02191-RA vs. NCBI nr
Match: KAG6578518.1 (Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 853.6 bits (2204), Expect = 7.9e-244
Identity = 444/485 (91.55%), Postives = 444/485 (91.55%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG            
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRALP 180

Query: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240
                                       WQQKDPCLNLPWKLKTFPI SESSPLYHTLQQ
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPILSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 446
           AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

BLAST of Carg02191-RA vs. NCBI nr
Match: XP_023551146.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 836.3 bits (2159), Expect = 1.3e-238
Identity = 438/486 (90.12%), Postives = 442/486 (90.95%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAAST-PPPHLNLAVSPAAPTVD 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAAST PPP LNLAVSPAAPTVD
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPPPLNLAVSPAAPTVD 60

Query: 61  FSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHS 120
           FSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHS
Sbjct: 61  FSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHS 120

Query: 121 FYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG----------- 180
           FYRHFCAGEDDTAVGKTVRRLH+VGLRSMLDYAIEYANDEESCDRNLHG           
Sbjct: 121 FYRHFCAGEDDTAVGKTVRRLHDVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRAL 180

Query: 181 -----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQ 240
                                        WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQ
Sbjct: 181 PPGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQ 240

Query: 241 QPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK 300
           QPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK
Sbjct: 241 QPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK 300

Query: 301 DHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DHNPIVYGTLQAYLKDAKDRLLLVTKEAN+MRVPLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DHNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLE 420
           IHNSIHDTHACYNSCASFLLDNIA GSSGAILATHNV+SGKLAAKKAYEIGIEKLK+KLE
Sbjct: 361 IHNSIHDTHACYNSCASFLLDNIAVGSSGAILATHNVDSGKLAAKKAYEIGIEKLKEKLE 420

Query: 421 FAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR 446
           FAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR
Sbjct: 421 FAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480

BLAST of Carg02191-RA vs. NCBI nr
Match: XP_022993279.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 820.5 bits (2118), Expect = 7.4e-234
Identity = 431/485 (88.87%), Postives = 434/485 (89.48%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYA NRLLNT     AAAFTSAASTPPP LNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYASNRLLNT-----AAAFTSAASTPPP-LNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARD+IFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDIIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNL G            
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLRGFLRTIEATRALP 180

Query: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240
                                       WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ
Sbjct: 181 PGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEAN+MRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIA GSSGAILATHNVESGKL AKKAYEIGIEKLK+KLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 446
           AQLYGMSEALSYGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 479

BLAST of Carg02191-RA vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 429.9 bits (1104), Expect = 3.7e-119
Identity = 225/463 (48.60%), Postives = 299/463 (64.58%), Query Frame = 0

Query: 26  NTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDFSDTKALFGSLSTLEVLHAAASLHVA 85
           +TA      +F   A  PP H       +   +D SD   LF S+ T ++L + A LH A
Sbjct: 31  STAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRSTAVLHAA 90

Query: 86  AVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFYRHFCAGEDDTAVGKTVRRLHN-VG 145
           A+GP+VD+G WVM+SKLMD  + R ++ G V+ +FY HFCAGED  A  + VR ++   G
Sbjct: 91  AIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRSVYEATG 150

Query: 146 LRSMLDYAIEYANDEESCDRNLH------------------------------------- 205
           L+ ML Y +E+A+D  SCD N+                                      
Sbjct: 151 LKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPISLLKRVS 210

Query: 206 ---GWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQ 265
               W+ K P   L WKLK+FP+FSESSPLYHT  +P PLT EE++ L+ +H R+ +IC+
Sbjct: 211 DLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGRIQEICR 270

Query: 266 SCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHN-PIVYGTLQAYLKDAKDRLLLV 325
            C ++NVPL IDAE T +QPAIDY  YS+AI+ N D + PIVY T+QAYL+DA +RL L 
Sbjct: 271 KCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGERLHLA 330

Query: 326 TKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIA 385
            + A +  VP+G KLVRGAYMSSE+ LA SLG +SP+H++I DTH+CYN C +FL++  +
Sbjct: 331 VQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTFLMEKAS 390

Query: 386 TGSS-GAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVS 445
            GS  G +LATHN +SG+LA++KA ++GI+K   K+EFAQLYGMS+ALS+GL+ AGF VS
Sbjct: 391 NGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKRAGFNVS 450

BLAST of Carg02191-RA vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 9.1e-118
Identity = 217/431 (50.35%), Postives = 285/431 (66.13%), Query Frame = 0

Query: 58  VDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVR 117
           +D SD   LF S+    +L + A LH  ++GP+VD+G W+M+SKLMD  + RD++   V+
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 118 HSFYRHFCAGEDDTAVGKTVRRLH-NVGLRSMLDYAIEYANDEESCDRNLH--------- 177
            +FY HFCAGED  A  + V  ++ + GL+ ML Y +E+A D  +CD N+          
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 178 -------------------------------GWQQKDPCLNLPWKLKTFPIFSESSPLYH 237
                                           WQ K+P   LPWKL +FP+FS  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 238 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 297
           T  +P PLT EE++ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI+
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 298 HNKDHN-PIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLG 357
            N D + PIVY T+QAYLKDA +RL L  +E+ +M VP+G KLVRGAYMSSE+KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 358 FESPIHNSIHDTHACYNSCASFLLDNIATGSS-GAILATHNVESGKLAAKKAYEIGIEKL 417
           ++SP+H++I +TH CYN C SFL++  + GS    ILATHN +SGKL A+KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 418 KKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLD 446
             K+EFAQLYGMS+ALS+GL+ AGF VSKYMP+G VD  +PYL+RRA ENRG++S   LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

BLAST of Carg02191-RA vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 5.1e-44
Identity = 137/432 (31.71%), Postives = 219/432 (50.69%), Query Frame = 0

Query: 62  DTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFY 121
           DT  L+ S ST E+      L V ++  + D     +N     + L++ + F  +++SF+
Sbjct: 145 DTSKLYVSKSTGELFFTFMILKVCSINFISDNSQKFLN-LFEKLGLSKPLNF-FIKYSFF 204

Query: 122 RHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAI-EYANDEESCDRNLHGWQQ------KDP 181
           + FCAGE          +L+ +G+ ++LDYAI E A   E  D       +      K+P
Sbjct: 205 KQFCAGETIRETEIFTEKLNKLGIGTILDYAIEELAGSSEGFDSVAENICETIRVAAKNP 264

Query: 182 -----CL-------------------NLPWKLKTFPIFSESSPLYHTLQQPNP------- 241
                C+                   N+   +   PI S +SPL   L Q +        
Sbjct: 265 TNSFSCVKFTGLVTPSVLEKMNTLVSNVTTNVSELPIESFNSPLDFYLNQSSSLMKQGSE 324

Query: 242 --LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDH 301
             LT+++ K ++    R+ KI Q C    VP+ +DAE +  Q AI + T S +I +NK+ 
Sbjct: 325 PLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTMSYSIKYNKE- 384

Query: 302 NPIVYGTLQAYLKDAKDRLL--LVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESP 361
            PI+Y T Q YL +  + L        + +    LG K+VRGAYM +ES+ +  L  E+P
Sbjct: 385 KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESERSQRLSTENP 444

Query: 362 IHNSIHDTHACYNSCASFLLDNIAT--GSSGAILATHNVESGKLAAKKAYEIGIEKLKKK 421
           +  +I DTH  YN+   FLL+ I +   S G ++A+HN +S  L  K   +  I+     
Sbjct: 445 VLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIKQYKIDPTNPN 504

Query: 422 LEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQL 448
           ++F QL+GM++ LS+ L +   ++ KY+PFG V+ V+PYL+RR  EN+G +  SN D++L
Sbjct: 505 IQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKGFI-GSNSDKEL 564

BLAST of Carg02191-RA vs. ExPASy Swiss-Prot
Match: Q9WU79 (Proline dehydrogenase 1, mitochondrial OS=Mus musculus OX=10090 GN=Prodh PE=1 SV=2)

HSP 1 Score: 148.7 bits (374), Expect = 1.7e-34
Identity = 88/242 (36.36%), Postives = 136/242 (56.20%), Query Frame = 0

Query: 205 TNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNPI 264
           T EE++ ++   QR+  + +   +A V L IDAE +  QPAI   T       N D  P 
Sbjct: 348 TEEEEQQMKRMLQRMDVLAKKAKEAGVRLMIDAEQSYFQPAISRLTLEMQRRFNVD-KPF 407

Query: 265 VYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSI 324
           ++ T Q YLKDA D + L  + A       G KLVRGAYM+ E   A+ +G+E PI+ + 
Sbjct: 408 IFNTFQCYLKDAYDNVTLDMELARREGWCFGAKLVRGAYMAQERVRAAEIGYEDPINPTY 467

Query: 325 HDTHACYNSCASFLLDNIATGSSGAIL-ATHNVESGKLAAKKAYEIGIEKLKKKLEFAQL 384
             T+A Y+ C +++L+ +   +   ++ A+HN ++      +  EIG+     ++ F QL
Sbjct: 468 EATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVHFTLCRMKEIGLHPADGQVCFGQL 527

Query: 385 YGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELG 444
            GM + +S+ L  AGF V KY+P+G V  V+PYL RRA EN  ++  +  +RQL+ +EL 
Sbjct: 528 LGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALENSSIMKGAQRERQLLWQELR 587

Query: 445 RR 446
           RR
Sbjct: 588 RR 588

BLAST of Carg02191-RA vs. ExPASy Swiss-Prot
Match: A6QQ74 (Hydroxyproline dehydrogenase OS=Bos taurus OX=9913 GN=PRODH2 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.8e-34
Identity = 123/422 (29.15%), Postives = 195/422 (46.21%), Query Frame = 0

Query: 74  EVLHAAASLHVAAVGPVVDVGMWVM--NSKLMDVELARDVIFGTVRHSFYRHFCAGEDDT 133
           E+  A   L + A  P+V  G+ +   + +L+   L+  ++    R S Y  F AGE   
Sbjct: 36  ELTRALLVLRLCAWPPLVTHGLALQAWSQRLLGSRLSGALL----RASIYGQFVAGETAE 95

Query: 134 AVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGW-------------------QQK 193
            V   V +L N+GLR +L  A+    + +S  +    W                   +  
Sbjct: 96  EVRSCVLQLQNLGLRPLL--AVPTEEEPDSAVKTGEAWYEGNLSAMLRCVDLSRGLLETP 155

Query: 194 DPCLNLPWKLKTFPIFS------------------ESSP--LYHTLQ-----QPNPLTNE 253
           DP  N   +LK   + S                  E SP  L   +      Q + L  E
Sbjct: 156 DPTGNALMQLKMTALMSTRLCKQLTSWVRRPGDSLELSPERLAEAMDSGQDLQVSCLNTE 215

Query: 254 EQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNK--DHNPIV 313
           + + LQ S  RL ++ Q      V L +DAE+T + PA+     + A   N   +  P V
Sbjct: 216 QTRHLQASLSRLHRVVQHARAQRVRLLVDAEYTSLNPALSLLVAALATRWNSSGEGGPWV 275

Query: 314 YGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIH 373
           + T QAYLKD  +RL    + A+   +  G+KLVRGAY+  E + A   G E P      
Sbjct: 276 WNTYQAYLKDTYERLRWDAEAADRAGLAFGVKLVRGAYLDKERETARLQGTEDPTQPDYE 335

Query: 374 DTHACYNSCASFLLDNIATGS--SGAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQL 433
            T   Y+ C   +L  ++        ++A+HN +S + A K+ +E+GI      + F QL
Sbjct: 336 ATSQSYSRCLELMLTQVSHRGPMCHLMVASHNEDSVRQATKRMWELGIPP-DGPVCFGQL 395

Query: 434 YGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRKELG 446
            GM + +S  L  AG+ V K +P+G+++ V+PYL+RRA+ENR +L  +  +++L+ +EL 
Sbjct: 396 LGMCDHVSLALGQAGYAVYKSIPYGSLEEVIPYLIRRAQENRSVLRGARREQELLSQELR 450

BLAST of Carg02191-RA vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 7.7e-245
Identity = 445/485 (91.75%), Postives = 445/485 (91.75%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG            
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRVLP 180

Query: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240
                                       WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 446
           AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

BLAST of Carg02191-RA vs. ExPASy TrEMBL
Match: A0A6J1JZR8 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.6e-234
Identity = 431/485 (88.87%), Postives = 434/485 (89.48%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MARSILSTQSKIPKNVFFYA NRLLNT     AAAFTSAASTPPP LNLAVSPAAPTVDF
Sbjct: 1   MARSILSTQSKIPKNVFFYASNRLLNT-----AAAFTSAASTPPP-LNLAVSPAAPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARD+IFGTVRHSF
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDIIFGTVRHSF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180
           YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNL G            
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLRGFLRTIEATRALP 180

Query: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240
                                       WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ
Sbjct: 181 PGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
           HNPIVYGTLQAYLKDAKDRLLLVTKEAN+MRVPLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HNSIHDTHACYNSCASFLLDNIA GSSGAILATHNVESGKL AKKAYEIGIEKLK+KLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 446
           AQLYGMSEALSYGLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 479

BLAST of Carg02191-RA vs. ExPASy TrEMBL
Match: A0A0A0KXN4 (Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 371/489 (75.87%), Postives = 412/489 (84.25%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSA-ASTPPP---HLNLAVSPAAP 60
           MA++I+STQSKI K VFFY FNR LNTAASS AAAFT+A A+T PP   HLN    P+ P
Sbjct: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLN---PPSTP 60

Query: 61  TVDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTV 120
           TVDF+D++ALFGS+ T ++LHA A+LH AAVGPVVDVGMWVMNSKLMDVEL RDV+ GTV
Sbjct: 61  TVDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTV 120

Query: 121 RHSFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG-------- 180
           +HSFYRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL G        
Sbjct: 121 KHSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEAT 180

Query: 181 --------------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYH 240
                                           WQQK+P  NLPWKL+T PIFSESSPLYH
Sbjct: 181 KSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYH 240

Query: 241 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 300
           TL+QP PLT EE+KSLQ+SH+RLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAII
Sbjct: 241 TLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 300

Query: 301 HNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGF 360
           HNKD NPIVYGT+QAYLKDAKDRLLL  KEA++++VPLGIKLVRGAYMSSESKLASSLGF
Sbjct: 301 HNKDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGF 360

Query: 361 ESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKK 420
           ESPIH++I DTH+CYN+CASFLLD+IA GS+GAILATHNVESGKLAA +AYEIGI KLK+
Sbjct: 361 ESPIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQ 420

Query: 421 KLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQ 446
           KLEFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDR+
Sbjct: 421 KLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRE 480

BLAST of Carg02191-RA vs. ExPASy TrEMBL
Match: A0A5D3BNA9 (Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002050 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.6e-202
Identity = 367/485 (75.67%), Postives = 406/485 (83.71%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MAR+++STQSKI K VF Y FNR LNTAASS AAAFT  A  PP HLN    P+ PTVDF
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFT--APPPPLHLN---PPSTPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           +D++ALFGS+ T ++LHA A+LH +A+GPVVDVGMWVMNSKLMDVEL RDV+ GTV+H+F
Sbjct: 61  TDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180
           YRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL G            
Sbjct: 121 YRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLP 180

Query: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240
                                       WQQK+P  NLPWKL+T PIFSESSPLYHTL+Q
Sbjct: 181 SGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           P PLT EE+KSLQISH+RLMKICQSCVD NVPLAIDAEHTK+QPAIDYFTYSAAIIHNKD
Sbjct: 241 PEPLTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLLL  KEA+++++PLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 RNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HN+I DTH+CYN+CASFLLD+IA GSSGAILATHNVESGKLAA +AYEIGI KLK+KLEF
Sbjct: 361 HNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 446
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

BLAST of Carg02191-RA vs. ExPASy TrEMBL
Match: A0A1S3CBE9 (Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.8e-201
Identity = 365/485 (75.26%), Postives = 406/485 (83.71%), Query Frame = 0

Query: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60
           MAR+++STQSKI K VF Y FNR LNTAASS AAAFT  A  PP HLN    P+ PTVDF
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFT--APPPPLHLN---PPSTPTVDF 60

Query: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120
           +D++ALFGS+ T ++LHA A+LH +A+GPVVDVGMWVMNSKLMDVEL RDV+ GTV+H+F
Sbjct: 61  TDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTF 120

Query: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHG------------ 180
           YRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL G            
Sbjct: 121 YRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLP 180

Query: 181 ----------------------------WQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240
                                       WQQK+P  NLPWKL+T PIFSESSPLYHTL+Q
Sbjct: 181 SGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQ 240

Query: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300
           P PLT EE+KSLQISH+RLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHNKD
Sbjct: 241 PEPLTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360
            NPIVYGT+QAYLKDAKDRLLL  KEA+++++PLGIKLVRGAYMSSESKLASSLGFESPI
Sbjct: 301 RNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420
           HN+I DTH+CYN+CASFLLD+IA GSSGAILATHNVESGKLAA +AYEIGI KLK+KLEF
Sbjct: 361 HNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEF 420

Query: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 446
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDRQLMRK
Sbjct: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMRK 480

BLAST of Carg02191-RA vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 429.9 bits (1104), Expect = 2.6e-120
Identity = 225/463 (48.60%), Postives = 299/463 (64.58%), Query Frame = 0

Query: 26  NTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDFSDTKALFGSLSTLEVLHAAASLHVA 85
           +TA      +F   A  PP H       +   +D SD   LF S+ T ++L + A LH A
Sbjct: 31  STAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRSTAVLHAA 90

Query: 86  AVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSFYRHFCAGEDDTAVGKTVRRLHN-VG 145
           A+GP+VD+G WVM+SKLMD  + R ++ G V+ +FY HFCAGED  A  + VR ++   G
Sbjct: 91  AIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRSVYEATG 150

Query: 146 LRSMLDYAIEYANDEESCDRNLH------------------------------------- 205
           L+ ML Y +E+A+D  SCD N+                                      
Sbjct: 151 LKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPISLLKRVS 210

Query: 206 ---GWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNPLTNEEQKSLQISHQRLMKICQ 265
               W+ K P   L WKLK+FP+FSESSPLYHT  +P PLT EE++ L+ +H R+ +IC+
Sbjct: 211 DLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGRIQEICR 270

Query: 266 SCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHN-PIVYGTLQAYLKDAKDRLLLV 325
            C ++NVPL IDAE T +QPAIDY  YS+AI+ N D + PIVY T+QAYL+DA +RL L 
Sbjct: 271 KCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAGERLHLA 330

Query: 326 TKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNSIHDTHACYNSCASFLLDNIA 385
            + A +  VP+G KLVRGAYMSSE+ LA SLG +SP+H++I DTH+CYN C +FL++  +
Sbjct: 331 VQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTFLMEKAS 390

Query: 386 TGSS-GAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQLYGMSEALSYGLRNAGFQVS 445
            GS  G +LATHN +SG+LA++KA ++GI+K   K+EFAQLYGMS+ALS+GL+ AGF VS
Sbjct: 391 NGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKRAGFNVS 450

BLAST of Carg02191-RA vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 425.2 bits (1092), Expect = 6.5e-119
Identity = 217/431 (50.35%), Postives = 285/431 (66.13%), Query Frame = 0

Query: 58  VDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVR 117
           +D SD   LF S+    +L + A LH  ++GP+VD+G W+M+SKLMD  + RD++   V+
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 118 HSFYRHFCAGEDDTAVGKTVRRLH-NVGLRSMLDYAIEYANDEESCDRNLH--------- 177
            +FY HFCAGED  A  + V  ++ + GL+ ML Y +E+A D  +CD N+          
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 178 -------------------------------GWQQKDPCLNLPWKLKTFPIFSESSPLYH 237
                                           WQ K+P   LPWKL +FP+FS  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 238 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 297
           T  +P PLT EE++ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI+
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 298 HNKDHN-PIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLG 357
            N D + PIVY T+QAYLKDA +RL L  +E+ +M VP+G KLVRGAYMSSE+KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 358 FESPIHNSIHDTHACYNSCASFLLDNIATGSS-GAILATHNVESGKLAAKKAYEIGIEKL 417
           ++SP+H++I +TH CYN C SFL++  + GS    ILATHN +SGKL A+KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 418 KKKLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLD 446
             K+EFAQLYGMS+ALS+GL+ AGF VSKYMP+G VD  +PYL+RRA ENRG++S   LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016079.13.5e-260100.00Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022939392.11.6e-24491.75proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata][more]
KAG6578518.17.9e-24491.55Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023551146.11.3e-23890.12proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_022993279.17.4e-23488.87proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P929833.7e-11948.60Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 P... [more]
Q6NKX19.1e-11850.35Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 P... [more]
Q86H285.1e-4431.71Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=p... [more]
Q9WU791.7e-3436.36Proline dehydrogenase 1, mitochondrial OS=Mus musculus OX=10090 GN=Prodh PE=1 SV... [more]
A6QQ742.8e-3429.15Hydroxyproline dehydrogenase OS=Bos taurus OX=9913 GN=PRODH2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FGN77.7e-24591.75Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1[more]
A0A6J1JZR83.6e-23488.87Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1[more]
A0A0A0KXN41.3e-20275.87Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1[more]
A0A5D3BNA93.6e-20275.67Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16... [more]
A0A1S3CBE91.8e-20175.26Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G30775.12.6e-12048.60Methylenetetrahydrofolate reductase family protein [more]
AT5G38710.16.5e-11950.35Methylenetetrahydrofolate reductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002872Proline dehydrogenase domainPFAMPF01619Pro_dhcoord: 135..429
e-value: 2.4E-72
score: 243.8
NoneNo IPR availableGENE3D3.20.20.220coord: 100..446
e-value: 9.0E-87
score: 293.6
NoneNo IPR availablePIRSRPIRSR000196-2PIRSR000196-2coord: 119..425
e-value: 3.6E-14
score: 50.5
NoneNo IPR availablePANTHERPTHR13914:SF25PROLINE DEHYDROGENASEcoord: 10..169
NoneNo IPR availablePANTHERPTHR13914:SF25PROLINE DEHYDROGENASEcoord: 169..445
IPR015659Proline oxidase familyPANTHERPTHR13914PROLINE OXIDASEcoord: 10..169
coord: 169..445
IPR029041FAD-linked oxidoreductase-likeSUPERFAMILY51730FAD-linked oxidoreductasecoord: 127..446

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg02191Carg02191gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg02191-RA:1Carg02191-RA:1exon
Carg02191-RA:2Carg02191-RA:2exon
Carg02191-RA:3Carg02191-RA:3exon
Carg02191-RA:4Carg02191-RA:4exon
Carg02191-RA:5Carg02191-RA:5exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg02191-RA:cdsCarg02191-RA:cds_5CDS
Carg02191-RA:cdsCarg02191-RA:cds_4CDS
Carg02191-RA:cdsCarg02191-RA:cds_3CDS
Carg02191-RA:cdsCarg02191-RA:cds_2CDS
Carg02191-RA:cdsCarg02191-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg02191-RACarg02191-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006562 proline catabolic process
biological_process GO:0010133 proline catabolic process to glutamate
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004657 proline dehydrogenase activity
molecular_function GO:0071949 FAD binding