Carg01118-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01118-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPyruvate decarboxylase
LocationCarg_Chr06: 1139058 .. 1142338 (-)
Sequence length2009
RNA-Seq ExpressionCarg01118-RA
SyntenyCarg01118-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTGGTTTTTCTCTTCCAAAACCGCTTTTGATTATCCAGCTGCCACTGCCACCCTTCCGTCTTGTATATATACAAACCTCTTCGTTTCCTTTCCATTCCATCGCTCAAAAACCACCGTTCTCTAATTCCCAAGCTCCCATTTTCCGATTCCGATTCCGATTCTCAGTCGATGGACACCAAAATCGGCTCCCTCGACGTTAGCAAACCCGCCAATGACGTGGTTTGTTGTCCATCCAATGGCTCTGTTTCCGCCATCCAAGACTCTGTCCCTACCGCCGCCGTCACCTCGTGGGATGCCACTCTCGGCGGCCACTTGGCACGACGGCTCGTCCAAATCGGTATCACCGATCTTTTCACTGTCCCCGGCGATTTCAACCTAACCCTTCTCGACCATTTAATCGACGAACCTAGACTCACCAATGTCGGGTGCTGTAACGAACTCAATGCTGGATACGCCGCCGATGGGTACGCTAGAGCCCGTGGTGTCGGCGCGTGTGTCGTCACGTTCTCCGTCGGCGGTCTTAGCGTCTTGAATGCCATTGCCGGAGCGTACAGTGAAAACTTGCCGGTGATTTGCATCGTCGGTGGCCCTAATTCGAACGATTATGGAACAAACAGAATCCTTCATCATACCATTGGGCTCCCTGACTTCAGCCAAGAACTCCGATGCTTTCAAACCGTCACTTGCTATCAGGTACTAAAGTAAACAAGATCTTGTTCCATAATTTTAATTTATTTTTTAGAGTTCTAAATTTTGTTGTTGTTTAGGCTGTGGTGAATCATCTTGAAGATGCTCATGAATTGATTGATACTGCCATTTCTACTGCCTTGAAAGAAAGCAAGCCTGTTTATATTAGTATCAGTTGTAATTTAGCTGGGCTTCCTCATCCCACCTTCAGCCGTGATCCAGTTCCATTTTACATCTCTCCCAAGTAAGTTCTGTAGCTCGAGTGGCTTTGCTCAGGACTTCTCCCGTGAGGGAGATGTATTATTTACAAACTCATGATCATTTTCTAAATTAGTCCGAGATGTATTATTTACTTATAAACTCATGATCATTTTCTAAATTAGTCCGTGCATGACTCCCTCCCAACCATACTTTCTGCCCCTGAGGCCTATGGGGGCCTATGAAGGCTATAGAGCTCTTGAATAGCCTCTCCCTAGAGTCCTCAAATGATCATTTTCTAAATTAGCCCATGCGGGACCCCTCCCTCCCAACCATCCTTTTCACCTCCCCGATGCCTATAGAGCCCTTGAATAGCCTCCCCTTAATCGAGGCTCGAACGCTCGACTCCTTCTCTAGGGTCTGGGACCTCCCTCTCAACCATCCTTTTCGCCTCCCATGAGGCCTATAGAACTCTTGAATAGCCTCCCCTGAGGCCTATAGAACTCTTGAATAGCCTCCCCTTAATCGAGGCTCGACTCCTTCTCTAGAGTCCTCGAATAAAGTACCCACTTTGTTCGACACATGATTCACTTTTGACTACACGTTCGAGGTTCACTTGAATAGTCTCCTCTTAATCGAGGCTTGAGTCTTTTTCTGGAGTCCTTGAATAAAATACACCCTTTGTTCGACGACATTTAAGGATTCTGATACCATTAGTAATTATGTACCTTCATAATGGTATGATATTATCCACCTTGAGCATAAGCTCTGATGGTTTTGCTTTGTGTTTCCCCAAAATGCTTCGTATCAATGGAGATGTATTTTTTTACTTATAAGCTGATAATCGTTCCCCGAGTAATGGGTTCTTTTCGTTTATGAAATTTAAGCTTAAAAATCATCCATTTATTCGTTATTAAACGGTTGCTAAGGACTGAATTGGTTTGAAATTATTGAATGGATTTGTGTGATTTGAACAGAATGAGCAATCCAAATGGGCTAGAGGCTGCAGTGGAGGCGGCAGCACAATTCTTGAACAAGGCAGTGAAGCCAGTGCTTGTTGGTGGGCCTAAATTGCGAGTTTCCAAGGCATGTGATGCCTTTGTGGAGCTTGCTGATGCTTCAAGCTACGCCATGGCCGTGATGCCATCGGCCAAGGGGCTTGTACCAGAGGACCATCCTCATTTCATTGGAACTTACTGGGGTGCTATCAGTACAGCCTTCTGTGCCGAGATCGTCGAGTCTGCCGATGCCTACTTGTTCGCTGGACCAATCTTCAATGACTACAGCTCTGTTGGCTACTCTCTGTTGCTCAAAAGGGAGAAGGCTGTGATCGTGGCGCCTGAACGTGTGACAATCGGCAATGGACCAACATTCGGTTGTGTTCTAATGAAAGATTTCCTTCGAGCTCTTGCGAAACGGATTAAGAGCAATATCAGCTCTTATGAAAACTACCATAGAATCTTTGTTCCTGATGGCGTTCCTGTGAGATTTGGGCCAAGGGAAGCCTTGAGAGTCAATGTTTTGTTCCAACATATTCAGAAGATGGTCAGTAGTCAATCGGCTGTGATTGCTGACACAGGAGATTCTTGGTTTAACTGCCAGAAACTTAAATTGCCAAAAGGCTGCGGGTAATGCATATTCAAATTCGTTCGTTCATTCGTTCGTTCGTTCATTCATTCAACTATTTCTGTGCAGATTTGAAATGGGATTTTCTCTTTTGGTTTGTAGATATGAGTTTCAAATGCAATATGGTTCTATTGGTTGGTCAGTTGGTGCCACTCTTGGATATGCTCAATCTGTACCAGAAAAAAGAGTGATTTCTTGCATTGGAGATGGAAGCTTCCAGGTAAGAATTGGTTCAATCGAGCTTTGACGCTCGACCCCATCGGAAAACGATCATCAGTTGATCATCGATACTATTTTACATTGCAGGTGACAGCACCAGACATATCGACAATGATGCGTCAGGGGCATAACACCATCATATTCCTGATAAACAATGGTGGCTACACCATAGAAGTTGAGATCCACGACGGGCCTTATAATGTGATCAAGAACTGGGACTACAAGGCATTGGTCGATGCGATCCACAACGGGGAAGGAAAGTGTTGGACGACGAAGGTGAGTTGGTATCGAATTTGGTTGGTCATTTGAGTTATGTTGAGGAGGAATTCTGTTTCGTGAACGAGTTTGTCGTTGCAGGTTCGATGCGAGGAAGAGCTTGTGGAGGCAATCGAGATCGCGACCGGAGACAAGAAGGACTGCTTGTGCTTCATTGAGGTGTTTGCTCACAAGGATGATACTAGCAAAGAGCTATTGGAATGGGGCTCAAGAGTCTCTGCTGCCAACAGCCGCCCTCCCAATCCTCAGTAAAACACAAAATAAAAGAGATCT

mRNA sequence

AAGTGGTTTTTCTCTTCCAAAACCGCTTTTGATTATCCAGCTGCCACTGCCACCCTTCCGTCTTGTATATATACAAACCTCTTCGTTTCCTTTCCATTCCATCGCTCAAAAACCACCGTTCTCTAATTCCCAAGCTCCCATTTTCCGATTCCGATTCCGATTCTCAGTCGATGGACACCAAAATCGGCTCCCTCGACGTTAGCAAACCCGCCAATGACGTGGTTTGTTGTCCATCCAATGGCTCTGTTTCCGCCATCCAAGACTCTGTCCCTACCGCCGCCGTCACCTCGTGGGATGCCACTCTCGGCGGCCACTTGGCACGACGGCTCGTCCAAATCGGTATCACCGATCTTTTCACTGTCCCCGGCGATTTCAACCTAACCCTTCTCGACCATTTAATCGACGAACCTAGACTCACCAATGTCGGGTGCTGTAACGAACTCAATGCTGGATACGCCGCCGATGGGTACGCTAGAGCCCGTGGTGTCGGCGCGTGTGTCGTCACGTTCTCCGTCGGCGGTCTTAGCGTCTTGAATGCCATTGCCGGAGCGTACAGTGAAAACTTGCCGGTGATTTGCATCGTCGGTGGCCCTAATTCGAACGATTATGGAACAAACAGAATCCTTCATCATACCATTGGGCTCCCTGACTTCAGCCAAGAACTCCGATGCTTTCAAACCGTCACTTGCTATCAGGCTGTGGTGAATCATCTTGAAGATGCTCATGAATTGATTGATACTGCCATTTCTACTGCCTTGAAAGAAAGCAAGCCTGTTTATATTAGTATCAGTTGTAATTTAGCTGGGCTTCCTCATCCCACCTTCAGCCGTGATCCAGTTCCATTTTACATCTCTCCCAAAATGAGCAATCCAAATGGGCTAGAGGCTGCAGTGGAGGCGGCAGCACAATTCTTGAACAAGGCAGTGAAGCCAGTGCTTGTTGGTGGGCCTAAATTGCGAGTTTCCAAGGCATGTGATGCCTTTGTGGAGCTTGCTGATGCTTCAAGCTACGCCATGGCCGTGATGCCATCGGCCAAGGGGCTTGTACCAGAGGACCATCCTCATTTCATTGGAACTTACTGGGGTGCTATCAGTACAGCCTTCTGTGCCGAGATCGTCGAGTCTGCCGATGCCTACTTGTTCGCTGGACCAATCTTCAATGACTACAGCTCTGTTGGCTACTCTCTGTTGCTCAAAAGGGAGAAGGCTGTGATCGTGGCGCCTGAACGTGTGACAATCGGCAATGGACCAACATTCGGTTGTGTTCTAATGAAAGATTTCCTTCGAGCTCTTGCGAAACGGATTAAGAGCAATATCAGCTCTTATGAAAACTACCATAGAATCTTTGTTCCTGATGGCGTTCCTGTGAGATTTGGGCCAAGGGAAGCCTTGAGAGTCAATGTTTTGTTCCAACATATTCAGAAGATGGTCAGTAGTCAATCGGCTGTGATTGCTGACACAGGAGATTCTTGGTTTAACTGCCAGAAACTTAAATTGCCAAAAGGCTGCGGATATGAGTTTCAAATGCAATATGGTTCTATTGGTTGGTCAGTTGGTGCCACTCTTGGATATGCTCAATCTGTACCAGAAAAAAGAGTGATTTCTTGCATTGGAGATGGAAGCTTCCAGGTGACAGCACCAGACATATCGACAATGATGCGTCAGGGGCATAACACCATCATATTCCTGATAAACAATGGTGGCTACACCATAGAAGTTGAGATCCACGACGGGCCTTATAATGTGATCAAGAACTGGGACTACAAGGCATTGGTCGATGCGATCCACAACGGGGAAGGAAAGTGTTGGACGACGAAGGTTCGATGCGAGGAAGAGCTTGTGGAGGCAATCGAGATCGCGACCGGAGACAAGAAGGACTGCTTGTGCTTCATTGAGGTGTTTGCTCACAAGGATGATACTAGCAAAGAGCTATTGGAATGGGGCTCAAGAGTCTCTGCTGCCAACAGCCGCCCTCCCAATCCTCAGTAAAACACAAAATAAAAGAGATCT

Coding sequence (CDS)

ATGGACACCAAAATCGGCTCCCTCGACGTTAGCAAACCCGCCAATGACGTGGTTTGTTGTCCATCCAATGGCTCTGTTTCCGCCATCCAAGACTCTGTCCCTACCGCCGCCGTCACCTCGTGGGATGCCACTCTCGGCGGCCACTTGGCACGACGGCTCGTCCAAATCGGTATCACCGATCTTTTCACTGTCCCCGGCGATTTCAACCTAACCCTTCTCGACCATTTAATCGACGAACCTAGACTCACCAATGTCGGGTGCTGTAACGAACTCAATGCTGGATACGCCGCCGATGGGTACGCTAGAGCCCGTGGTGTCGGCGCGTGTGTCGTCACGTTCTCCGTCGGCGGTCTTAGCGTCTTGAATGCCATTGCCGGAGCGTACAGTGAAAACTTGCCGGTGATTTGCATCGTCGGTGGCCCTAATTCGAACGATTATGGAACAAACAGAATCCTTCATCATACCATTGGGCTCCCTGACTTCAGCCAAGAACTCCGATGCTTTCAAACCGTCACTTGCTATCAGGCTGTGGTGAATCATCTTGAAGATGCTCATGAATTGATTGATACTGCCATTTCTACTGCCTTGAAAGAAAGCAAGCCTGTTTATATTAGTATCAGTTGTAATTTAGCTGGGCTTCCTCATCCCACCTTCAGCCGTGATCCAGTTCCATTTTACATCTCTCCCAAAATGAGCAATCCAAATGGGCTAGAGGCTGCAGTGGAGGCGGCAGCACAATTCTTGAACAAGGCAGTGAAGCCAGTGCTTGTTGGTGGGCCTAAATTGCGAGTTTCCAAGGCATGTGATGCCTTTGTGGAGCTTGCTGATGCTTCAAGCTACGCCATGGCCGTGATGCCATCGGCCAAGGGGCTTGTACCAGAGGACCATCCTCATTTCATTGGAACTTACTGGGGTGCTATCAGTACAGCCTTCTGTGCCGAGATCGTCGAGTCTGCCGATGCCTACTTGTTCGCTGGACCAATCTTCAATGACTACAGCTCTGTTGGCTACTCTCTGTTGCTCAAAAGGGAGAAGGCTGTGATCGTGGCGCCTGAACGTGTGACAATCGGCAATGGACCAACATTCGGTTGTGTTCTAATGAAAGATTTCCTTCGAGCTCTTGCGAAACGGATTAAGAGCAATATCAGCTCTTATGAAAACTACCATAGAATCTTTGTTCCTGATGGCGTTCCTGTGAGATTTGGGCCAAGGGAAGCCTTGAGAGTCAATGTTTTGTTCCAACATATTCAGAAGATGGTCAGTAGTCAATCGGCTGTGATTGCTGACACAGGAGATTCTTGGTTTAACTGCCAGAAACTTAAATTGCCAAAAGGCTGCGGATATGAGTTTCAAATGCAATATGGTTCTATTGGTTGGTCAGTTGGTGCCACTCTTGGATATGCTCAATCTGTACCAGAAAAAAGAGTGATTTCTTGCATTGGAGATGGAAGCTTCCAGGTGACAGCACCAGACATATCGACAATGATGCGTCAGGGGCATAACACCATCATATTCCTGATAAACAATGGTGGCTACACCATAGAAGTTGAGATCCACGACGGGCCTTATAATGTGATCAAGAACTGGGACTACAAGGCATTGGTCGATGCGATCCACAACGGGGAAGGAAAGTGTTGGACGACGAAGGTTCGATGCGAGGAAGAGCTTGTGGAGGCAATCGAGATCGCGACCGGAGACAAGAAGGACTGCTTGTGCTTCATTGAGGTGTTTGCTCACAAGGATGATACTAGCAAAGAGCTATTGGAATGGGGCTCAAGAGTCTCTGCTGCCAACAGCCGCCCTCCCAATCCTCAGTAA

Protein sequence

MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITDLFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAAVEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFIGTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGPTFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMVSSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCIGDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHNGEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSRPPNPQ
Homology
BLAST of Carg01118-RA vs. NCBI nr
Match: KAG7027885.1 (Pyruvate decarboxylase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1239.9 bits (3207), Expect = 0.0e+00
Identity = 605/605 (100.00%), Postives = 605/605 (100.00%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD
Sbjct: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV
Sbjct: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH
Sbjct: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA
Sbjct: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI
Sbjct: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP
Sbjct: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV
Sbjct: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI
Sbjct: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN
Sbjct: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. NCBI nr
Match: XP_022949483.1 (pyruvate decarboxylase 2-like [Cucurbita moschata])

HSP 1 Score: 1238.0 bits (3202), Expect = 0.0e+00
Identity = 604/605 (99.83%), Postives = 604/605 (99.83%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD
Sbjct: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV
Sbjct: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH
Sbjct: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYI PKMSNPNGLEAA
Sbjct: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYIPPKMSNPNGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI
Sbjct: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP
Sbjct: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV
Sbjct: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI
Sbjct: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN
Sbjct: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. NCBI nr
Match: KAG6596333.1 (Pyruvate decarboxylase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 602/605 (99.50%), Postives = 603/605 (99.67%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD
Sbjct: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV
Sbjct: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH
Sbjct: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYI PKMSNPNGLEAA
Sbjct: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYIPPKMSNPNGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYA+AVMPSAKGLVPEDHPHFI
Sbjct: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAVAVMPSAKGLVPEDHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIV PERVTIGNGP
Sbjct: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVEPERVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV
Sbjct: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI
Sbjct: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN
Sbjct: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. NCBI nr
Match: XP_023540069.1 (pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1226.5 bits (3172), Expect = 0.0e+00
Identity = 598/605 (98.84%), Postives = 601/605 (99.34%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDT IGSLDVSKPANDVVCCPSNGSVSAIQDSVPTA VTSWDATLGGHLARRLVQIGITD
Sbjct: 1   MDTIIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAPVTSWDATLGGHLARRLVQIGITD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV
Sbjct: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH
Sbjct: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           L DAHELIDTAISTALKESKPVYISISCNLAGLPHPTF+RDPVPFYISP+MSNPNGLEAA
Sbjct: 181 LGDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFNRDPVPFYISPRMSNPNGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYA+AVMPSAKGLVPEDHPHFI
Sbjct: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAVAVMPSAKGLVPEDHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIV PERVTIGNGP
Sbjct: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVEPERVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV
Sbjct: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI
Sbjct: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN
Sbjct: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. NCBI nr
Match: XP_022971428.1 (pyruvate decarboxylase 2-like [Cucurbita maxima])

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 591/605 (97.69%), Postives = 597/605 (98.68%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDT+IGSLDVSKPANDVVCCPSNGSVS IQDSVPTAAVTSWDATLGGHLARRLVQIGITD
Sbjct: 51  MDTRIGSLDVSKPANDVVCCPSNGSVSTIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 110

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV
Sbjct: 111 LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 170

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH
Sbjct: 171 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 230

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           L DAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNP GLEAA
Sbjct: 231 LGDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPTGLEAA 290

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYA+AVMPSAKGLVPEDHPHFI
Sbjct: 291 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAVAVMPSAKGLVPEDHPHFI 350

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIV PERVTIGNGP
Sbjct: 351 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVEPERVTIGNGP 410

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKM+
Sbjct: 411 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMI 470

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SSQ+AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI
Sbjct: 471 SSQTAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 530

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVT  DISTM+RQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDY ALVDAIHN
Sbjct: 531 GDGSFQVTVSDISTMIRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAIHN 590

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEELVEAI IATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR
Sbjct: 591 GEGKCWTTKVRCEEELVEAISIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 650

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 651 PPNPQ 655

BLAST of Carg01118-RA vs. ExPASy Swiss-Prot
Match: O82647 (Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 8.2e-300
Identity = 489/607 (80.56%), Postives = 546/607 (89.95%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVT--SWDATLGGHLARRLVQIGI 60
           MDTKIGS+D  KP N  VC P+NG+V+ I +SVP++A+T    DATLG HLARRLVQ G+
Sbjct: 1   MDTKIGSIDDCKPTNGDVCSPTNGTVATIHNSVPSSAITINYCDATLGRHLARRLVQAGV 60

Query: 61  TDLFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGL 120
           TD+F+VPGDFNLTLLDHL+ EP L  +GCCNELNAGYAADGYAR+RGVGACVVTF+VGGL
Sbjct: 61  TDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGL 120

Query: 121 SVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180
           SVLNAIAGAYSENLP+ICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV
Sbjct: 121 SVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180

Query: 181 NHLEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLE 240
           N+L+DAHE ID AISTALKESKPVYIS+SCNLA +PH TFSRDPVPF ++P++SN  GLE
Sbjct: 181 NNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDPVPFSLAPRLSNKMGLE 240

Query: 241 AAVEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPH 300
           AAVEA  +FLNKAVKPV+VGGPKLRV+KACDAFVELADAS YA+A+MPSAKG VPE HPH
Sbjct: 241 AAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYALAMMPSAKGFVPEHHPH 300

Query: 301 FIGTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGN 360
           FIGTYWGA+ST FC+EIVESADAY+FAGPIFNDYSSVGYSLLLK+EKA++V P+R+T+ N
Sbjct: 301 FIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIVVQPDRITVAN 360

Query: 361 GPTFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQK 420
           GPTFGC+LM DF R L+KR+K N ++YENYHRIFVP+G P++   RE LRVN +FQHIQK
Sbjct: 361 GPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQK 420

Query: 421 MVSSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIS 480
           M+SS++AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRV++
Sbjct: 421 MLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLA 480

Query: 481 CIGDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAI 540
            IGDGSFQVT  DISTM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y  LVDAI
Sbjct: 481 FIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAI 540

Query: 541 HNGEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAAN 600
           HNGEG CWT KVR EEELVEAI  AT +KKDCLCFIEV  HKDDTSKELLEWGSRVSAAN
Sbjct: 541 HNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKDDTSKELLEWGSRVSAAN 600

Query: 601 SRPPNPQ 606
           SRPPNPQ
Sbjct: 601 SRPPNPQ 607

BLAST of Carg01118-RA vs. ExPASy Swiss-Prot
Match: Q9FFT4 (Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 4.6e-295
Identity = 487/607 (80.23%), Postives = 542/607 (89.29%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVP--TAAVTSWDATLGGHLARRLVQIGI 60
           MDTKIGS+D   P N  +  P NG VS +Q++ P  +  V+  DATLG +LARRLV+IG+
Sbjct: 1   MDTKIGSIDACNPTNHDIGGPPNGGVSTVQNTSPLHSTTVSPCDATLGRYLARRLVEIGV 60

Query: 61  TDLFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGL 120
           TD+F+VPGDFNLTLLDHLI EP L  +GCCNELNAGYAADGYAR+RGVGACVVTF+VGGL
Sbjct: 61  TDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGL 120

Query: 121 SVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180
           SVLNAIAGAYSENLP+ICIVGGPNSNDYGTNRILHHTIGLPDF+QELRCFQ VTC+QAV+
Sbjct: 121 SVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQAVI 180

Query: 181 NHLEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLE 240
           N+LE+AHELIDTAISTALKESKPVYISISCNL  +P PTFSR PVPF +  K+SN  GL+
Sbjct: 181 NNLEEAHELIDTAISTALKESKPVYISISCNLPAIPLPTFSRHPVPFMLPMKVSNQIGLD 240

Query: 241 AAVEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPH 300
           AAVEAAA+FLNKAVKPVLVGGPK+RV+KA DAFVELADAS Y +AVMPSAKG VPE H H
Sbjct: 241 AAVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKH 300

Query: 301 FIGTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGN 360
           FIGTYWGA+STAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLK+EKA+IV P+RVTIGN
Sbjct: 301 FIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGN 360

Query: 361 GPTFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQK 420
           GP FGCVLMKDFL  LAKRIK N +SYENYHRI+VP+G P+R  P E+LRVNVLFQHIQ 
Sbjct: 361 GPAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQN 420

Query: 421 MVSSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIS 480
           M+SS+SAV+A+TGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGATLGYAQ++P +RVI+
Sbjct: 421 MLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIA 480

Query: 481 CIGDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAI 540
           CIGDGSFQVTA D+STM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y A V+AI
Sbjct: 481 CIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAI 540

Query: 541 HNGEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAAN 600
           HNGEGKCWT KVRCEEELV+AI  AT ++K+  CFIEV  HKDDTSKELLEWGSRVSAAN
Sbjct: 541 HNGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAAN 600

Query: 601 SRPPNPQ 606
           SRPPNPQ
Sbjct: 601 SRPPNPQ 607

BLAST of Carg01118-RA vs. ExPASy Swiss-Prot
Match: Q9M040 (Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 1.8e-294
Identity = 484/605 (80.00%), Postives = 538/605 (88.93%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIG++D  KP    +  P + +V+ IQDS P    T+ ++TLG HL+RRLVQ G+TD
Sbjct: 1   MDTKIGAIDTCKPTTGDIGSPPSNAVATIQDSAP--ITTTSESTLGRHLSRRLVQAGVTD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           +F+VPGDFNLTLLDHLI EP L N+GCCNELNAGYAADGYAR+RGVGACVVTF+VGGLSV
Sbjct: 61  VFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSND+GTNRILHHTIGLPDFSQELRCFQTVTCYQAVVN+
Sbjct: 121 LNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNN 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHE ID AI+TALKESKPVYISISCNLA  PHPTF+RDPVPF ++P+MSN  GLEAA
Sbjct: 181 LEDAHEQIDKAIATALKESKPVYISISCNLAATPHPTFARDPVPFDLTPRMSNTMGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEA  +FLNKAVKPV+VGGPKLRV+KA +AF+ELADAS Y +AVMPS KGLVPE+HPHFI
Sbjct: 241 VEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGA+ST FC+EIVESADAY+FAGPIFNDYSSVGYSLLLK+EKA+IV P+RV + NGP
Sbjct: 301 GTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVVVANGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLM DF R LAKR+K N ++YENY RIFVP+G P++  P E LRVN +FQHIQKM+
Sbjct: 361 TFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKPLKCKPGEPLRVNAMFQHIQKML 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SS++AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRV+S I
Sbjct: 421 SSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTA DISTM+R G   IIFLINNGGYTIEVEIHDGPYNVIKNW+Y  LVDAIHN
Sbjct: 481 GDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVR EEELVEAI+ AT +KKD LCFIEV  HKDDTSKELLEWGSRVSAAN R
Sbjct: 541 GEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 603

BLAST of Carg01118-RA vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 996.5 bits (2575), Expect = 1.3e-289
Identity = 479/607 (78.91%), Postives = 535/607 (88.14%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPA--NDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGI 60
           M+T IGS+D +  A  N  V CP++ +V     S   A V++ +A+LG HLARRLVQ+G+
Sbjct: 1   METHIGSVDGAAAAADNGAVGCPAS-AVGCPMTSARPAPVSAGEASLGRHLARRLVQVGV 60

Query: 61  TDLFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGL 120
           +D+F VPGDFNLTLLDHLI EP L  VGCCNELNAGYAADGYARARGVGAC VTF+VGGL
Sbjct: 61  SDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGYAADGYARARGVGACAVTFTVGGL 120

Query: 121 SVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180
           SVLNAIAGAYSENLPVICI GGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTC+QAVV
Sbjct: 121 SVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCHQAVV 180

Query: 181 NHLEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLE 240
            +LEDAHE IDTAI+TAL+ESKPVY+SISCNL GLPHPTFSRDPVPF+++P++SN  GLE
Sbjct: 181 TNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPHPTFSRDPVPFFLAPRLSNKMGLE 240

Query: 241 AAVEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPH 300
           AAVEA  +FLNKAVKPVLVGGPKLRV+KA  AFV+L DAS YA AVMPSAKGLVPE HPH
Sbjct: 241 AAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLVDASGYAYAVMPSAKGLVPETHPH 300

Query: 301 FIGTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGN 360
           FIGTYWGA+STAFCAEIVESADAYLFAGPIFNDYSSVGYS LLK++KA+IV PERV +GN
Sbjct: 301 FIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSFLLKKDKAIIVQPERVIVGN 360

Query: 361 GPTFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQK 420
           GP FGCV+MK+FL  LAKR+  N ++YENY RIFVP+G P+   P E LRVNVLF+H+QK
Sbjct: 361 GPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQK 420

Query: 421 MVSSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIS 480
           M++S SAVIA+TGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGA LGYAQ   +KRVI+
Sbjct: 421 MLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALLGYAQGAKDKRVIA 480

Query: 481 CIGDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAI 540
           CIGDGSFQVTA D+STM+R   N+IIFLINNGGYTIEVEIHDGPYNVIKNW+Y  LVDAI
Sbjct: 481 CIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAI 540

Query: 541 HNGEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAAN 600
           HNGEGKCWT+KV+CEEEL EAI +A G+KKDCLCFIEV AHKDDTSKELLEWGSRVSAAN
Sbjct: 541 HNGEGKCWTSKVKCEEELTEAIGMALGEKKDCLCFIEVIAHKDDTSKELLEWGSRVSAAN 600

Query: 601 SRPPNPQ 606
           SRPPNPQ
Sbjct: 601 SRPPNPQ 606

BLAST of Carg01118-RA vs. ExPASy Swiss-Prot
Match: Q9M039 (Pyruvate decarboxylase 3 OS=Arabidopsis thaliana OX=3702 GN=PDC3 PE=2 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 9.4e-288
Identity = 477/592 (80.57%), Postives = 528/592 (89.19%), Query Frame = 0

Query: 16  DVVCCPSNGSVSAIQDSVPTAA--VTSWDATLGGHLARRLVQIGITDLFTVPGDFNLTLL 75
           DV   PSNG V+ IQDS PTAA  + S  ATLG HL+RRLVQ G+TD+FTVPGDFNL+LL
Sbjct: 2   DVRSLPSNG-VATIQDSAPTAATILGSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLL 61

Query: 76  DHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSVLNAIAGAYSENLP 135
           D LI  P L N+GCCNELNAGYAADGYAR+RGVGACVVTF+VGGLSVLNAIAGAYSENLP
Sbjct: 62  DQLIANPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLP 121

Query: 136 VICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHELIDTAIS 195
           VICIVGGPNSND+GTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHE ID AI+
Sbjct: 122 VICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIA 181

Query: 196 TALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAAVEAAAQFLNKAVK 255
           TAL+ESKPVYISISCNLA +PHPTF+  PVPF ++P++SN + LEAAVEA  +FLNKAVK
Sbjct: 182 TALRESKPVYISISCNLAAIPHPTFASYPVPFDLTPRLSNKDCLEAAVEATLEFLNKAVK 241

Query: 256 PVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFIGTYWGAISTAFCA 315
           PV+VGGPKLRV+KA DAFVELADAS Y +AVMPSAKG VPE+HPHFIGTYWGA+ST FC+
Sbjct: 242 PVMVGGPKLRVAKARDAFVELADASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCS 301

Query: 316 EIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGPTFGCVLMKDFLRA 375
           EIVESADAY+FAGPIFNDYSSVGYSLLLK+EKA+IV P+ V + NGPTFGCV M +F R 
Sbjct: 302 EIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRE 361

Query: 376 LAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMVSSQSAVIADTGDS 435
           LAKR+K N ++YENYHRIFVP+G P++  PRE LR+N +FQHIQKM+S+++AVIA+TGDS
Sbjct: 362 LAKRVKPNKTAYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDS 421

Query: 436 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCIGDGSFQVTAPDIS 495
           WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRV+S IGDGSFQVTA D+S
Sbjct: 422 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVS 481

Query: 496 TMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHNGEGKCWTTKVRCE 555
           TM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y  LVDAIHNGEGKCWTTKVR E
Sbjct: 482 TMIRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYE 541

Query: 556 EELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSRPPNPQ 606
           EELVEAI  AT +KKD LCFIEV  HKDDTSKELLEWGSRVSAAN RPPNPQ
Sbjct: 542 EELVEAINTATLEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNPQ 592

BLAST of Carg01118-RA vs. ExPASy TrEMBL
Match: A0A6J1GC79 (Pyruvate decarboxylase OS=Cucurbita moschata OX=3662 GN=LOC111452811 PE=3 SV=1)

HSP 1 Score: 1238.0 bits (3202), Expect = 0.0e+00
Identity = 604/605 (99.83%), Postives = 604/605 (99.83%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD
Sbjct: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV
Sbjct: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH
Sbjct: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYI PKMSNPNGLEAA
Sbjct: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYIPPKMSNPNGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI
Sbjct: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP
Sbjct: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV
Sbjct: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI
Sbjct: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN
Sbjct: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. ExPASy TrEMBL
Match: A0A6J1I6U2 (Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111470156 PE=3 SV=1)

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 591/605 (97.69%), Postives = 597/605 (98.68%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDT+IGSLDVSKPANDVVCCPSNGSVS IQDSVPTAAVTSWDATLGGHLARRLVQIGITD
Sbjct: 51  MDTRIGSLDVSKPANDVVCCPSNGSVSTIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 110

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV
Sbjct: 111 LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 170

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH
Sbjct: 171 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 230

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           L DAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNP GLEAA
Sbjct: 231 LGDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPTGLEAA 290

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYA+AVMPSAKGLVPEDHPHFI
Sbjct: 291 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAVAVMPSAKGLVPEDHPHFI 350

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIV PERVTIGNGP
Sbjct: 351 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVEPERVTIGNGP 410

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKM+
Sbjct: 411 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMI 470

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SSQ+AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI
Sbjct: 471 SSQTAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 530

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVT  DISTM+RQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDY ALVDAIHN
Sbjct: 531 GDGSFQVTVSDISTMIRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAIHN 590

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEELVEAI IATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR
Sbjct: 591 GEGKCWTTKVRCEEELVEAISIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 650

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 651 PPNPQ 655

BLAST of Carg01118-RA vs. ExPASy TrEMBL
Match: A0A6J1F2E8 (Pyruvate decarboxylase OS=Cucurbita moschata OX=3662 GN=LOC111441524 PE=3 SV=1)

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 527/605 (87.11%), Postives = 569/605 (94.05%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIGSLD  KPAN+ VCCPSNGSV  IQ+SVP+  VTS +ATLG HLARRLVQIGITD
Sbjct: 1   MDTKIGSLDACKPANNAVCCPSNGSVCTIQNSVPSTVVTSSEATLGRHLARRLVQIGITD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           +F+VPGDFNLTLLDHLI EP L N+GCCNELNAGYAADGYAR+RGVGACVVTF+VGGLSV
Sbjct: 61  VFSVPGDFNLTLLDHLIAEPGLNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLP+ICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTC+QAVVN+
Sbjct: 121 LNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCFQAVVNN 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHELIDTAISTALKESKPVYISI CNL G+PHPTFSRDPVPF +SPK+SNP+GLEAA
Sbjct: 181 LEDAHELIDTAISTALKESKPVYISIGCNLPGIPHPTFSRDPVPFSLSPKVSNPHGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRV+K CDAFVELADA  YA++VMPSAKGLVPE HPHFI
Sbjct: 241 VEAAAQFLNKAVKPVLVGGPKLRVAKGCDAFVELADACGYALSVMPSAKGLVPEHHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGA+ST+FCAEIVESAD+YLFAGPIFNDYSSVGYSLLLKREKA+IV P+RVTIGNGP
Sbjct: 301 GTYWGAVSTSFCAEIVESADSYLFAGPIFNDYSSVGYSLLLKREKAIIVQPDRVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFL+AL+KR+  N ++YENYHRIFVP+G P++  P+EALRVN+LFQHIQKM+
Sbjct: 361 TFGCVLMKDFLQALSKRVNKNTTAYENYHRIFVPEGHPLKSEPKEALRVNILFQHIQKML 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           S Q+AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+VPEKRVI+CI
Sbjct: 421 SGQTAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIACI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTA DISTM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y ALVDAIHN
Sbjct: 481 GDGSFQVTAQDISTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEEL+EAI  ATGDKKD LCFIEV AHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVRCEEELIEAIGTATGDKKDSLCFIEVIAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. ExPASy TrEMBL
Match: A0A6J1J2I6 (Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111482096 PE=3 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 526/605 (86.94%), Postives = 569/605 (94.05%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIGSLD  KPAN+ VCCPSNGSV  IQ+SVP+  VTS +ATLG HLARRLVQIGITD
Sbjct: 1   MDTKIGSLDACKPANNAVCCPSNGSVCTIQNSVPSTVVTSSEATLGRHLARRLVQIGITD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           +F+VPGDFNLTLLDHLI EP L N+GCCNELNAGYAADGYAR+RGVGACVVTF+VGGLSV
Sbjct: 61  VFSVPGDFNLTLLDHLIAEPGLNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLP+ICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTC+QAVVN+
Sbjct: 121 LNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCFQAVVNN 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHELIDTAISTALKESKPVYISI CNL G+PHPTFSRDPVPF +SPK+SNP+GLEAA
Sbjct: 181 LEDAHELIDTAISTALKESKPVYISIGCNLPGIPHPTFSRDPVPFSLSPKVSNPHGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAAQFLNKAVKPVLVGGPKLRV+K CDAFVELADA  YA++VMPSAKGLVPE HPHFI
Sbjct: 241 VEAAAQFLNKAVKPVLVGGPKLRVAKGCDAFVELADACGYALSVMPSAKGLVPEHHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGA+ST+FCAEIVESAD+YLFAGPIFNDYSSVGYSLLLKREKA+IV P+RVTIGNGP
Sbjct: 301 GTYWGAVSTSFCAEIVESADSYLFAGPIFNDYSSVGYSLLLKREKAIIVQPDRVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFL+AL+KR+  N ++YENYHRIFVP+G P++  P+EALRVN+LFQHIQKM+
Sbjct: 361 TFGCVLMKDFLQALSKRVNKNTTAYENYHRIFVPEGHPLKSEPKEALRVNILFQHIQKML 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           S Q+AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+VPEKRVI+CI
Sbjct: 421 SGQTAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIACI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTA DISTM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y ALVDAIHN
Sbjct: 481 GDGSFQVTAQDISTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVRCEEEL+EAI  ATGDK+D LCFIEV AHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVRCEEELIEAIGTATGDKEDSLCFIEVIAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. ExPASy TrEMBL
Match: A0A0A0LF82 (Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_3G730980 PE=3 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 527/605 (87.11%), Postives = 566/605 (93.55%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIGSLD  KP N+VVCCPSNGSV  IQ+SVP+  V+S DATLG HLARRLVQIG+TD
Sbjct: 1   MDTKIGSLDTCKPQNNVVCCPSNGSVCTIQNSVPSTVVSSSDATLGRHLARRLVQIGVTD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           +FTVPGDFNLTLLDHLI EP L N+GCCNELNAGYAADGYAR RGVGACVVTF+VGGLSV
Sbjct: 61  VFTVPGDFNLTLLDHLIAEPALNNIGCCNELNAGYAADGYARCRGVGACVVTFTVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLP+ICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTC+QAVVN+
Sbjct: 121 LNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCFQAVVNN 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHELIDTAISTALKESKPVYISISCNL G+PHPTFSRDPVPF +SPK+SNP GLEAA
Sbjct: 181 LEDAHELIDTAISTALKESKPVYISISCNLPGIPHPTFSRDPVPFSLSPKVSNPKGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEAAA FLNKAVKPVLVGGPK+RV+KAC AFVELADA  YA+AVMPSAKGLVPE HPHFI
Sbjct: 241 VEAAAHFLNKAVKPVLVGGPKMRVAKACHAFVELADACGYALAVMPSAKGLVPEHHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGA+STAFCAEIVESADAYLF GPIFNDYSSVGYSLLLKREKA+IV P+RVTIGNGP
Sbjct: 301 GTYWGAVSTAFCAEIVESADAYLFTGPIFNDYSSVGYSLLLKREKAIIVQPDRVTIGNGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLMKDFL+AL+KR+ +N ++YENYHRIFVP+G P++  P+E LRVN+LFQHIQKM+
Sbjct: 361 TFGCVLMKDFLQALSKRVNNNTTAYENYHRIFVPEGCPLKAEPKEPLRVNILFQHIQKML 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           S Q+AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+VPEKRVI+CI
Sbjct: 421 SRQTAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIACI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTA DISTM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y ALVDAIHN
Sbjct: 481 GDGSFQVTAQDISTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTALVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKV+CEEELVEAIE AT  KKDCLCFIEV AHKDDTSKELLEWGSRVSAANSR
Sbjct: 541 GEGKCWTTKVQCEEELVEAIETATDAKKDCLCFIEVIAHKDDTSKELLEWGSRVSAANSR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 605

BLAST of Carg01118-RA vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 1030.4 bits (2663), Expect = 5.8e-301
Identity = 489/607 (80.56%), Postives = 546/607 (89.95%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVT--SWDATLGGHLARRLVQIGI 60
           MDTKIGS+D  KP N  VC P+NG+V+ I +SVP++A+T    DATLG HLARRLVQ G+
Sbjct: 1   MDTKIGSIDDCKPTNGDVCSPTNGTVATIHNSVPSSAITINYCDATLGRHLARRLVQAGV 60

Query: 61  TDLFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGL 120
           TD+F+VPGDFNLTLLDHL+ EP L  +GCCNELNAGYAADGYAR+RGVGACVVTF+VGGL
Sbjct: 61  TDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGL 120

Query: 121 SVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180
           SVLNAIAGAYSENLP+ICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV
Sbjct: 121 SVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180

Query: 181 NHLEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLE 240
           N+L+DAHE ID AISTALKESKPVYIS+SCNLA +PH TFSRDPVPF ++P++SN  GLE
Sbjct: 181 NNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDPVPFSLAPRLSNKMGLE 240

Query: 241 AAVEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPH 300
           AAVEA  +FLNKAVKPV+VGGPKLRV+KACDAFVELADAS YA+A+MPSAKG VPE HPH
Sbjct: 241 AAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYALAMMPSAKGFVPEHHPH 300

Query: 301 FIGTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGN 360
           FIGTYWGA+ST FC+EIVESADAY+FAGPIFNDYSSVGYSLLLK+EKA++V P+R+T+ N
Sbjct: 301 FIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIVVQPDRITVAN 360

Query: 361 GPTFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQK 420
           GPTFGC+LM DF R L+KR+K N ++YENYHRIFVP+G P++   RE LRVN +FQHIQK
Sbjct: 361 GPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKCESREPLRVNTMFQHIQK 420

Query: 421 MVSSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIS 480
           M+SS++AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRV++
Sbjct: 421 MLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLA 480

Query: 481 CIGDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAI 540
            IGDGSFQVT  DISTM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y  LVDAI
Sbjct: 481 FIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAI 540

Query: 541 HNGEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAAN 600
           HNGEG CWT KVR EEELVEAI  AT +KKDCLCFIEV  HKDDTSKELLEWGSRVSAAN
Sbjct: 541 HNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKDDTSKELLEWGSRVSAAN 600

Query: 601 SRPPNPQ 606
           SRPPNPQ
Sbjct: 601 SRPPNPQ 607

BLAST of Carg01118-RA vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 1014.6 bits (2622), Expect = 3.3e-296
Identity = 487/607 (80.23%), Postives = 542/607 (89.29%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVP--TAAVTSWDATLGGHLARRLVQIGI 60
           MDTKIGS+D   P N  +  P NG VS +Q++ P  +  V+  DATLG +LARRLV+IG+
Sbjct: 1   MDTKIGSIDACNPTNHDIGGPPNGGVSTVQNTSPLHSTTVSPCDATLGRYLARRLVEIGV 60

Query: 61  TDLFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGL 120
           TD+F+VPGDFNLTLLDHLI EP L  +GCCNELNAGYAADGYAR+RGVGACVVTF+VGGL
Sbjct: 61  TDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYARSRGVGACVVTFTVGGL 120

Query: 121 SVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180
           SVLNAIAGAYSENLP+ICIVGGPNSNDYGTNRILHHTIGLPDF+QELRCFQ VTC+QAV+
Sbjct: 121 SVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFTQELRCFQAVTCFQAVI 180

Query: 181 NHLEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLE 240
           N+LE+AHELIDTAISTALKESKPVYISISCNL  +P PTFSR PVPF +  K+SN  GL+
Sbjct: 181 NNLEEAHELIDTAISTALKESKPVYISISCNLPAIPLPTFSRHPVPFMLPMKVSNQIGLD 240

Query: 241 AAVEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPH 300
           AAVEAAA+FLNKAVKPVLVGGPK+RV+KA DAFVELADAS Y +AVMPSAKG VPE H H
Sbjct: 241 AAVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYGLAVMPSAKGQVPEHHKH 300

Query: 301 FIGTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGN 360
           FIGTYWGA+STAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLK+EKA+IV P+RVTIGN
Sbjct: 301 FIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVTIGN 360

Query: 361 GPTFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQK 420
           GP FGCVLMKDFL  LAKRIK N +SYENYHRI+VP+G P+R  P E+LRVNVLFQHIQ 
Sbjct: 361 GPAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRDNPNESLRVNVLFQHIQN 420

Query: 421 MVSSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIS 480
           M+SS+SAV+A+TGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGATLGYAQ++P +RVI+
Sbjct: 421 MLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAMPNRRVIA 480

Query: 481 CIGDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAI 540
           CIGDGSFQVTA D+STM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y A V+AI
Sbjct: 481 CIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTAFVEAI 540

Query: 541 HNGEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAAN 600
           HNGEGKCWT KVRCEEELV+AI  AT ++K+  CFIEV  HKDDTSKELLEWGSRVSAAN
Sbjct: 541 HNGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKDDTSKELLEWGSRVSAAN 600

Query: 601 SRPPNPQ 606
           SRPPNPQ
Sbjct: 601 SRPPNPQ 607

BLAST of Carg01118-RA vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 1012.7 bits (2617), Expect = 1.2e-295
Identity = 484/605 (80.00%), Postives = 538/605 (88.93%), Query Frame = 0

Query: 1   MDTKIGSLDVSKPANDVVCCPSNGSVSAIQDSVPTAAVTSWDATLGGHLARRLVQIGITD 60
           MDTKIG++D  KP    +  P + +V+ IQDS P    T+ ++TLG HL+RRLVQ G+TD
Sbjct: 1   MDTKIGAIDTCKPTTGDIGSPPSNAVATIQDSAP--ITTTSESTLGRHLSRRLVQAGVTD 60

Query: 61  LFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSV 120
           +F+VPGDFNLTLLDHLI EP L N+GCCNELNAGYAADGYAR+RGVGACVVTF+VGGLSV
Sbjct: 61  VFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSV 120

Query: 121 LNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNH 180
           LNAIAGAYSENLPVICIVGGPNSND+GTNRILHHTIGLPDFSQELRCFQTVTCYQAVVN+
Sbjct: 121 LNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNN 180

Query: 181 LEDAHELIDTAISTALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAA 240
           LEDAHE ID AI+TALKESKPVYISISCNLA  PHPTF+RDPVPF ++P+MSN  GLEAA
Sbjct: 181 LEDAHEQIDKAIATALKESKPVYISISCNLAATPHPTFARDPVPFDLTPRMSNTMGLEAA 240

Query: 241 VEAAAQFLNKAVKPVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFI 300
           VEA  +FLNKAVKPV+VGGPKLRV+KA +AF+ELADAS Y +AVMPS KGLVPE+HPHFI
Sbjct: 241 VEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHFI 300

Query: 301 GTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGP 360
           GTYWGA+ST FC+EIVESADAY+FAGPIFNDYSSVGYSLLLK+EKA+IV P+RV + NGP
Sbjct: 301 GTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVVVANGP 360

Query: 361 TFGCVLMKDFLRALAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMV 420
           TFGCVLM DF R LAKR+K N ++YENY RIFVP+G P++  P E LRVN +FQHIQKM+
Sbjct: 361 TFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKPLKCKPGEPLRVNAMFQHIQKML 420

Query: 421 SSQSAVIADTGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCI 480
           SS++AVIA+TGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRV+S I
Sbjct: 421 SSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFI 480

Query: 481 GDGSFQVTAPDISTMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHN 540
           GDGSFQVTA DISTM+R G   IIFLINNGGYTIEVEIHDGPYNVIKNW+Y  LVDAIHN
Sbjct: 481 GDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHN 540

Query: 541 GEGKCWTTKVRCEEELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSR 600
           GEGKCWTTKVR EEELVEAI+ AT +KKD LCFIEV  HKDDTSKELLEWGSRVSAAN R
Sbjct: 541 GEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGR 600

Query: 601 PPNPQ 606
           PPNPQ
Sbjct: 601 PPNPQ 603

BLAST of Carg01118-RA vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 990.3 bits (2559), Expect = 6.6e-289
Identity = 477/592 (80.57%), Postives = 528/592 (89.19%), Query Frame = 0

Query: 16  DVVCCPSNGSVSAIQDSVPTAA--VTSWDATLGGHLARRLVQIGITDLFTVPGDFNLTLL 75
           DV   PSNG V+ IQDS PTAA  + S  ATLG HL+RRLVQ G+TD+FTVPGDFNL+LL
Sbjct: 2   DVRSLPSNG-VATIQDSAPTAATILGSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLL 61

Query: 76  DHLIDEPRLTNVGCCNELNAGYAADGYARARGVGACVVTFSVGGLSVLNAIAGAYSENLP 135
           D LI  P L N+GCCNELNAGYAADGYAR+RGVGACVVTF+VGGLSVLNAIAGAYSENLP
Sbjct: 62  DQLIANPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLP 121

Query: 136 VICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHELIDTAIS 195
           VICIVGGPNSND+GTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHE ID AI+
Sbjct: 122 VICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIA 181

Query: 196 TALKESKPVYISISCNLAGLPHPTFSRDPVPFYISPKMSNPNGLEAAVEAAAQFLNKAVK 255
           TAL+ESKPVYISISCNLA +PHPTF+  PVPF ++P++SN + LEAAVEA  +FLNKAVK
Sbjct: 182 TALRESKPVYISISCNLAAIPHPTFASYPVPFDLTPRLSNKDCLEAAVEATLEFLNKAVK 241

Query: 256 PVLVGGPKLRVSKACDAFVELADASSYAMAVMPSAKGLVPEDHPHFIGTYWGAISTAFCA 315
           PV+VGGPKLRV+KA DAFVELADAS Y +AVMPSAKG VPE+HPHFIGTYWGA+ST FC+
Sbjct: 242 PVMVGGPKLRVAKARDAFVELADASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCS 301

Query: 316 EIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVAPERVTIGNGPTFGCVLMKDFLRA 375
           EIVESADAY+FAGPIFNDYSSVGYSLLLK+EKA+IV P+ V + NGPTFGCV M +F R 
Sbjct: 302 EIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRE 361

Query: 376 LAKRIKSNISSYENYHRIFVPDGVPVRFGPREALRVNVLFQHIQKMVSSQSAVIADTGDS 435
           LAKR+K N ++YENYHRIFVP+G P++  PRE LR+N +FQHIQKM+S+++AVIA+TGDS
Sbjct: 362 LAKRVKPNKTAYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDS 421

Query: 436 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVISCIGDGSFQVTAPDIS 495
           WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRV+S IGDGSFQVTA D+S
Sbjct: 422 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVS 481

Query: 496 TMMRQGHNTIIFLINNGGYTIEVEIHDGPYNVIKNWDYKALVDAIHNGEGKCWTTKVRCE 555
           TM+R G  TIIFLINNGGYTIEVEIHDGPYNVIKNW+Y  LVDAIHNGEGKCWTTKVR E
Sbjct: 482 TMIRNGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYE 541

Query: 556 EELVEAIEIATGDKKDCLCFIEVFAHKDDTSKELLEWGSRVSAANSRPPNPQ 606
           EELVEAI  AT +KKD LCFIEV  HKDDTSKELLEWGSRVSAAN RPPNPQ
Sbjct: 542 EELVEAINTATLEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNPQ 592

BLAST of Carg01118-RA vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 89.4 bits (220), Expect = 1.1e-17
Identity = 106/481 (22.04%), Postives = 198/481 (41.16%), Query Frame = 0

Query: 49  LARRLVQIGITDLFTVPGDFNLTLLDHLIDEPRLTNVGCCNELNAGYAADGYARARG-VG 108
           L   L + G+  +F  PG  ++ +   L     + NV   +E    +AA+GYAR+ G  G
Sbjct: 103 LVEALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPG 162

Query: 109 ACVVTFSVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRC 168
            C+ T   G  ++++ +A A  +++P++ I G       GT+      I        +  
Sbjct: 163 ICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPI--------VEV 222

Query: 169 FQTVTCYQAVVNHLEDAHELIDTAISTALK-ESKPVYISISCNL-AGLPHPTFSRD-PVP 228
            +++T +  +V  +ED   +I+ A   A      PV + +  ++   L  P + +   +P
Sbjct: 223 TRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLP 282

Query: 229 FYISPKMSNPNGLEAAVEAAAQFLNKAVKPVL-VGGPKLRVSKACDAFVELADASSYAMA 288
            Y+S     P   ++ +E   + ++++ KPVL VGG  L  S     FVEL   +   +A
Sbjct: 283 GYMSRMPKPPE--DSHLEQIVRLISESKKPVLYVGGGCLNSSDELGRFVEL---TGIPVA 342

Query: 289 VMPSAKGLVPEDHP---HFIGTYWGAISTAFCAEIVESADAYLFAGPIFNDYSSVGYSLL 348
                 G  P D     H +G +     T +    VE +D  L  G  F+D  +      
Sbjct: 343 STLMGLGSYPCDDELSLHMLGMH----GTVYANYAVEHSDLLLAFGVRFDDRVTGKLEAF 402

Query: 349 LKREKAVIVAPERVTIGNGPTFGCVLMKDFLRAL----------AKRIKSNISSYENYHR 408
             R K V +  +   IG   T    +  D   AL          A+ +K +   + N   
Sbjct: 403 ASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWRNELN 462

Query: 409 IFVPDGVPVRFGP-REALRVNVLFQHIQKMVSSQSAVIADTGD-SWFNCQKLKLPKGCGY 468
           +      P+ F    EA+      + + ++   ++ +    G    +  Q     K   +
Sbjct: 463 V-QKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAAQFYNYKKPRQW 522

Query: 469 EFQMQYGSIGWSVGATLGYAQSVPEKRVISCIGDGSFQVTAPDISTMMRQGHNTIIFLIN 510
                 G++G+ + A +G + + P+  V+   GDGSF +   +++T+  +     + L+N
Sbjct: 523 LSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVKVLLLN 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027885.10.0e+00100.00Pyruvate decarboxylase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022949483.10.0e+0099.83pyruvate decarboxylase 2-like [Cucurbita moschata][more]
KAG6596333.10.0e+0099.50Pyruvate decarboxylase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023540069.10.0e+0098.84pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022971428.10.0e+0097.69pyruvate decarboxylase 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O826478.2e-30080.56Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1[more]
Q9FFT44.6e-29580.23Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1[more]
Q9M0401.8e-29480.00Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1[more]
A2XFI31.3e-28978.91Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=... [more]
Q9M0399.4e-28880.57Pyruvate decarboxylase 3 OS=Arabidopsis thaliana OX=3702 GN=PDC3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GC790.0e+0099.83Pyruvate decarboxylase OS=Cucurbita moschata OX=3662 GN=LOC111452811 PE=3 SV=1[more]
A0A6J1I6U20.0e+0097.69Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111470156 PE=3 SV=1[more]
A0A6J1F2E80.0e+0087.11Pyruvate decarboxylase OS=Cucurbita moschata OX=3662 GN=LOC111441524 PE=3 SV=1[more]
A0A6J1J2I60.0e+0086.94Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111482096 PE=3 SV=1[more]
A0A0A0LF820.0e+0087.11Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_3G730980 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G33070.15.8e-30180.56Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G54960.13.3e-29680.23pyruvate decarboxylase-2 [more]
AT5G01320.11.2e-29580.00Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G01330.16.6e-28980.57pyruvate decarboxylase-3 [more]
AT3G48560.11.1e-1722.04chlorsulfuron/imidazolinone resistant 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePIRSFPIRSF036565Pyruvt_ip_decrbcoord: 39..600
e-value: 6.2E-206
score: 682.9
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 1..605
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 241..358
e-value: 5.3E-20
score: 71.6
NoneNo IPR availableGENE3D3.40.50.970coord: 403..587
e-value: 1.2E-52
score: 180.1
NoneNo IPR availableGENE3D3.40.50.970coord: 41..217
e-value: 1.7E-60
score: 205.7
NoneNo IPR availableGENE3D3.40.50.1220coord: 230..393
e-value: 1.2E-40
score: 140.8
NoneNo IPR availablePIRSRPIRSR036565-2PIRSR036565-2coord: 44..587
e-value: 8.1E-174
score: 576.6
NoneNo IPR availablePANTHERPTHR43452:SF24PYRUVATE DECARBOXYLASE-RELATEDcoord: 1..605
NoneNo IPR availableCDDcd02005TPP_PDC_IPDCcoord: 406..588
e-value: 2.8437E-80
score: 248.988
NoneNo IPR availableCDDcd07038TPP_PYR_PDC_IPDC_likecoord: 48..208
e-value: 4.52897E-91
score: 276.298
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 451..576
e-value: 1.5E-14
score: 54.1
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 44..214
e-value: 3.0E-34
score: 118.1
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 408..600
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 44..223
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 231..380

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg01118Carg01118gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg01118-RA:6Carg01118-RA:6exon
Carg01118-RA:5Carg01118-RA:5exon
Carg01118-RA:4Carg01118-RA:4exon
Carg01118-RA:3Carg01118-RA:3exon
Carg01118-RA:2Carg01118-RA:2exon
Carg01118-RA:1Carg01118-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg01118-RA:three_prime_utrCarg01118-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg01118-RA:cdsCarg01118-RA:cds_6CDS
Carg01118-RA:cdsCarg01118-RA:cds_5CDS
Carg01118-RA:cdsCarg01118-RA:cds_4CDS
Carg01118-RA:cdsCarg01118-RA:cds_3CDS
Carg01118-RA:cdsCarg01118-RA:cds_2CDS
Carg01118-RA:cdsCarg01118-RA:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg01118-RA:five_prime_utrCarg01118-RA:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg01118-RACarg01118-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001666 response to hypoxia
cellular_component GO:0005829 cytosol
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0004737 pyruvate decarboxylase activity