CaUC03G053780.1 (mRNA) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G053780.1
TypemRNA
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionCitrulline--aspartate ligase
LocationCiama_Chr03: 4288454 .. 4292170 (-)
Sequence length1836
RNA-Seq ExpressionCaUC03G053780.1
SyntenyCaUC03G053780.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCCGACGGCCGTCGCTAGTTTTAAACCCTAGCGTCTTAGCCATTTTCAGAAAGGTGATTGCCAAAACTTTGTTCATCTTTGAAAATTTGTGAAGATCACGACAATCGCCGCCGACGCCATGGCTCAGATAATGGGAGGCTCTCTGTTCTCATCTGCTCCCGTTGGGTTTCACCAACCCAAAACAGGTATTTCGTTTGAATTCTTATTATTATTTTTCTGTTATTCAAAGAGTAAGATTGGAAATGGAAGTGAAAATGGGAATGGGAATGGGAACGGGAATGGACTGTTTACGGTAACTTAATTCGGGTTTCTGGCTACTCTGTGATTTTTCTTTCGGGAATACGAATGGATTAGTGTAATGTCGACGGTAGAGAATATGGTTCTATTGAGTTTTGTGGTTTTTAAAATGCATTATCTGAGGTTGCAGTTTTGTTTTTATTTTCAGAGGCAGTGGCGAGTTATTGCAGAATTTCTGGCCTGACGAAGTTGCATTCATTTCAAGAGGCATTTGCATCAATAATCCTCTCCTTCTTATCCTTAAATGATTTTTATCCATATCAGCCTGTTTTTATCTCATTCTTGGACTTATTCAATTGTATTCGTGGCTGCAGCTGGGTGCAAAAACGTGTGAAATCCATGGCAATGGCATAGCTAGTAACAGGGTTGGTGTTCTGCGGTCGAATAAGAAAAGCGGTGAGGTTCCCATTGTTGCTTGGTCTGCGCTTAATTATCTCCTCCTCCCTGGGTAGTTTTGGAGAAGCAGAGGGAGTTTTATCAGATTGAGCTTTTAATCATGGAGTCACTCTATTAGTAGTTTTGTATAGATAGTATAGTTGTGTTTATATTTACTCTACAAGAGCAGCCAAGTGGTATAAAGTGTATTATTAATACTAATTTTGTTCTACTGTTTTAGCTCTGTGATCGAGATGCTTTGTAAGATCTGTTTTTGAGGTTTTCCCTTTATATCGAGTTGATAAAAATTTTATCCTTTCTTTTTGAATGGATTCAGTTATTCGGGCAATATTGCAGAGTGAGAGAGAGATAGAAACTACCAATGGTATAGGAACTAGAGGTTTGCGTGGGCAGTTGAACAAGGTTGTTCTTGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGTAGGCCCCTTGGCTGATGTTTAATCTTGTTCATTGATCTAGCCCTGAACTTATAAATTATCAAATACTCATTCAGTAAGTAGTAATAGTTGCATGATTTGTGGACATTGAAGAATTCAGAGATAGCTGATACTGGCTCTTCAGTACATCGACTTCTTATGAGTTATAACCCTTCCCTTTGCTTGTACTGCGTGGATTTTGCTTTGAGCTTTCAGTTGTTCTTTCTATGTCATCTCCTTCTGAATTATTTTCTTCCTATGGCCATTAACTATTGCATGTATTGCAGGGAGAATTATGGTTGCGAAGTTGTATGTTTCACGGCTGATGTTGGTCAAGTATGCAATTAGCATTGGTGAATTAGTTTTTTTTTCTTTTTTTTTTTAATATTGTTTTTAAATAAAAGTTCTATATATATATATAGGATAGCCACTTGACTGAAAACCCTGTGAAATTGATCTTTTTGCATCTCTTTGTGGGTCTTAAACCTGTAGGGCGTAAAGGAATTGGAAGGTTTGGAAAAGAAGGCCAGAGCAAGTGGGGCTTGTCAATTGGTAGTGAAGGACTTGAAGGAAGAGTTTGTAAGCGACTTCATATTTCCTTGCTTACGTGCTGGTGCAATTTATGAAAGGAAATACCTGCTTGGGACATCAATGGCCCGTCCAGTTATTGCGAAGGTAATTTCTAACTTTAGTATCATAGTTACACTGATAGAGTATCATGGGCAAAAAAAAAGTTCTAACTGTAGTATTGCTACGGGTAGTAGTCCTTACTTATAAGTTATATTAGCAATGTGGATTTCCCAACGATAAATAAATTTAAGGTTCCAAGTGACTTGGAGCCTCCATTCACATTTATCATGTTCATGGTTTTTACTTGTTTGTTTGGTATTTGGTATTTGGTAATTCCTTTGCACTTCTTTTGATATTGAAAAAATAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCTGTTTCTCATGGATGCACTGGGAAAGGAAATGATCAGGTATGTATGCCCTTACACCGTTATCTGGCTATGAGCACTCTTTTCCCTGATGAATACAATTGGTATCTTATGATAAATCTAATCTAGGTACGCTTTGAGTTGGCCTTCTTTGCTCTAAATCCCAATTTAAATGTGGTGGCACCGTGGAGGGAATGGGATATTACTGGAAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATATACCTGTTCCAGTGACAAAGAAATCAATATACAGTCGAGACAGGAACTTATGGCATCTTAGTCATGAGGTAATGGTGATTAGTTTTGATTGATTCATCATTCGTCTAAGTTGTGGATTGTTTTTGTTTTAGTTTGTCTATCAAGTTTAAATCAACCTAAGTGACGATTATATGCTAGGATGTATCAAGAAACTGGGTGGATTTTGACCTCTTTATTAATTTTAGCTCTTTCTGATGTTCTCTTGTGCATTGTTACTGAAGGGAGACGTTTTGGAGGATCCAGCAAATGAACCTAAAAAGGACATGTACATGATGTCTACGGATCCAGAAGATGCACCAAACGAACCAGAGTAAAATTCCTTTCTTTAACTCCAAATCTTTGCAATAATGTTTCTATTATTGAATCTGTCTTCAACCATTCAAACTTCTATCAACGACAGTTGGTTGTGTAAAAGGACTAATCGGTCAATCTAGAATCAAGTCAATTCTATTTGCTACACACAGCTGGAAAATTAAGAGTTCTGACTCATTTTATTTGCTTAATTTGTGGGCCATACCTTAAGTCTCTTTGGTGGGTGTTTGCCATTTCTGTTCAACGAGCTCAACATGCCATCATCTTTTGCAGATATGTGGAAATAGGAATTGTGGGAGGTCTCCCTGTATCCGTCAATGGCGAGATACTTTCACCAGCTTCTCTTCTAGCCAAAGTAAACGACATTGGTGGAAAGCATGGAATTGGGAGAATTGACATGGTTGAAAACCGTCTGGTGGGTATGAAAAGTAGAGGTGTCTATGAAACCCCAGGAGGAACCATCCTTTTCACAGCCACCCGTGAGCTCGAGTCTCTAACACTTGACCGAGAGACGATTCAAGTGAAAGACTCATTGGCTCTCAAATATGCAGAGCTGGTTTATGCAGGAAGATGGTTCGACCCTCTTCGTGAATCCATGGATGCTTTCATGGAGAAGATCACAGAGACCACCACTGGCTCAGTCACTCTCAAGCTCTACAAGGGTTCCGTCCATGTGGCCAGCCGCCGGAGTCTGTACAGTCTTTATAGGGAAGATATCTCCTCCTTTGAAAATGGGCAGATTTACAATCAGGCTGATGCTGCTGGTTTCATTCGCCTTTATGGCCTTCCCACTAGAGTCCGGGCAATGCTCCATAAGGACTTTTGATCCCTCACTCTGATAATGGATGAGCCTTTGGCACAAGAATCAATAAGGTAATTTAATTAGTCGATATGTCTGTCTTTATTTGGTGGGCTGTTACAATTTGTACATGTATTGTTTTTGTAATGTAACCAAGGTTAGTTTTTTTTTTATTAATGGGATTAGGGTTTGCATTTAG

mRNA sequence

GGCCGACGGCCGTCGCTAGTTTTAAACCCTAGCGTCTTAGCCATTTTCAGAAAGGTGATTGCCAAAACTTTGTTCATCTTTGAAAATTTGTGAAGATCACGACAATCGCCGCCGACGCCATGGCTCAGATAATGGGAGGCTCTCTGTTCTCATCTGCTCCCGTTGGGTTTCACCAACCCAAAACAGAGGCAGTGGCGAGTTATTGCAGAATTTCTGGCCTGACGAAGTTGCATTCATTTCAAGAGGCATTTGCATCAATAATCCTCTCCTTCTTATCCTTAAATGATTTTTATCCATATCAGCCTCTGGGTGCAAAAACGTGTGAAATCCATGGCAATGGCATAGCTAGTAACAGGGTTGGTGTTCTGCGGTCGAATAAGAAAAGCGTTATTCGGGCAATATTGCAGAGTGAGAGAGAGATAGAAACTACCAATGGTATAGGAACTAGAGGTTTGCGTGGGCAGTTGAACAAGGTTGTTCTTGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGGAGAATTATGGTTGCGAAGTTGTATGTTTCACGGCTGATGTTGGTCAAGGCGTAAAGGAATTGGAAGGTTTGGAAAAGAAGGCCAGAGCAAGTGGGGCTTGTCAATTGGTAGTGAAGGACTTGAAGGAAGAGTTTGTAAGCGACTTCATATTTCCTTGCTTACGTGCTGGTGCAATTTATGAAAGGAAATACCTGCTTGGGACATCAATGGCCCGTCCAGTTATTGCGAAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCTGTTTCTCATGGATGCACTGGGAAAGGAAATGATCAGGTACGCTTTGAGTTGGCCTTCTTTGCTCTAAATCCCAATTTAAATGTGGTGGCACCGTGGAGGGAATGGGATATTACTGGAAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATATACCTGTTCCAGTGACAAAGAAATCAATATACAGTCGAGACAGGAACTTATGGCATCTTAGTCATGAGGGAGACGTTTTGGAGGATCCAGCAAATGAACCTAAAAAGGACATGTACATGATGTCTACGGATCCAGAAGATGCACCAAACGAACCAGAATATGTGGAAATAGGAATTGTGGGAGGTCTCCCTGTATCCGTCAATGGCGAGATACTTTCACCAGCTTCTCTTCTAGCCAAAGTAAACGACATTGGTGGAAAGCATGGAATTGGGAGAATTGACATGGTTGAAAACCGTCTGGTGGGTATGAAAAGTAGAGGTGTCTATGAAACCCCAGGAGGAACCATCCTTTTCACAGCCACCCGTGAGCTCGAGTCTCTAACACTTGACCGAGAGACGATTCAAGTGAAAGACTCATTGGCTCTCAAATATGCAGAGCTGGTTTATGCAGGAAGATGGTTCGACCCTCTTCGTGAATCCATGGATGCTTTCATGGAGAAGATCACAGAGACCACCACTGGCTCAGTCACTCTCAAGCTCTACAAGGGTTCCGTCCATGTGGCCAGCCGCCGGAGTCTGTACAGTCTTTATAGGGAAGATATCTCCTCCTTTGAAAATGGGCAGATTTACAATCAGGCTGATGCTGCTGGTTTCATTCGCCTTTATGGCCTTCCCACTAGAGTCCGGGCAATGCTCCATAAGGACTTTTGATCCCTCACTCTGATAATGGATGAGCCTTTGGCACAAGAATCAATAAGGTAATTTAATTAGTCGATATGTCTGTCTTTATTTGGTGGGCTGTTACAATTTGTACATGTATTGTTTTTGTAATGTAACCAAGGTTAGTTTTTTTTTTATTAATGGGATTAGGGTTTGCATTTAG

Coding sequence (CDS)

ATGGCTCAGATAATGGGAGGCTCTCTGTTCTCATCTGCTCCCGTTGGGTTTCACCAACCCAAAACAGAGGCAGTGGCGAGTTATTGCAGAATTTCTGGCCTGACGAAGTTGCATTCATTTCAAGAGGCATTTGCATCAATAATCCTCTCCTTCTTATCCTTAAATGATTTTTATCCATATCAGCCTCTGGGTGCAAAAACGTGTGAAATCCATGGCAATGGCATAGCTAGTAACAGGGTTGGTGTTCTGCGGTCGAATAAGAAAAGCGTTATTCGGGCAATATTGCAGAGTGAGAGAGAGATAGAAACTACCAATGGTATAGGAACTAGAGGTTTGCGTGGGCAGTTGAACAAGGTTGTTCTTGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGGAGAATTATGGTTGCGAAGTTGTATGTTTCACGGCTGATGTTGGTCAAGGCGTAAAGGAATTGGAAGGTTTGGAAAAGAAGGCCAGAGCAAGTGGGGCTTGTCAATTGGTAGTGAAGGACTTGAAGGAAGAGTTTGTAAGCGACTTCATATTTCCTTGCTTACGTGCTGGTGCAATTTATGAAAGGAAATACCTGCTTGGGACATCAATGGCCCGTCCAGTTATTGCGAAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCTGTTTCTCATGGATGCACTGGGAAAGGAAATGATCAGGTACGCTTTGAGTTGGCCTTCTTTGCTCTAAATCCCAATTTAAATGTGGTGGCACCGTGGAGGGAATGGGATATTACTGGAAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATATACCTGTTCCAGTGACAAAGAAATCAATATACAGTCGAGACAGGAACTTATGGCATCTTAGTCATGAGGGAGACGTTTTGGAGGATCCAGCAAATGAACCTAAAAAGGACATGTACATGATGTCTACGGATCCAGAAGATGCACCAAACGAACCAGAATATGTGGAAATAGGAATTGTGGGAGGTCTCCCTGTATCCGTCAATGGCGAGATACTTTCACCAGCTTCTCTTCTAGCCAAAGTAAACGACATTGGTGGAAAGCATGGAATTGGGAGAATTGACATGGTTGAAAACCGTCTGGTGGGTATGAAAAGTAGAGGTGTCTATGAAACCCCAGGAGGAACCATCCTTTTCACAGCCACCCGTGAGCTCGAGTCTCTAACACTTGACCGAGAGACGATTCAAGTGAAAGACTCATTGGCTCTCAAATATGCAGAGCTGGTTTATGCAGGAAGATGGTTCGACCCTCTTCGTGAATCCATGGATGCTTTCATGGAGAAGATCACAGAGACCACCACTGGCTCAGTCACTCTCAAGCTCTACAAGGGTTCCGTCCATGTGGCCAGCCGCCGGAGTCTGTACAGTCTTTATAGGGAAGATATCTCCTCCTTTGAAAATGGGCAGATTTACAATCAGGCTGATGCTGCTGGTTTCATTCGCCTTTATGGCCTTCCCACTAGAGTCCGGGCAATGCTCCATAAGGACTTTTGA

Protein sequence

MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPYQPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVNDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDF
Homology
BLAST of CaUC03G053780.1 vs. NCBI nr
Match: XP_038895641.1 (argininosuccinate synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 948.0 bits (2449), Expect = 3.4e-272
Identity = 476/513 (92.79%), Postives = 482/513 (93.96%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFS APVGFH  KTEAVASYCR+SGLTKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSYAPVGFHPSKTEAVASYCRVSGLTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAKTCEIHGN IASNR GVL+ NKKSVI+AILQSEREIETTNGIGTRGLRGQLNKVV
Sbjct: 61  --LGAKTCEIHGNSIASNRAGVLQLNKKSVIQAILQSEREIETTNGIGTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQG KELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGAKELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIV GLPVSVNGE+LSPASLLAKVN
Sbjct: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVRGLPVSVNGEVLSPASLLAKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGS TLKLYKGSVHVASRRSLYSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSATLKLYKGSVHVASRRSLYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 514
           SFENGQIYNQADA GFIRLYGLPTRVRAMLHKD
Sbjct: 481 SFENGQIYNQADADGFIRLYGLPTRVRAMLHKD 493

BLAST of CaUC03G053780.1 vs. NCBI nr
Match: XP_004152522.1 (argininosuccinate synthase, chloroplastic [Cucumis sativus] >KGN64401.1 hypothetical protein Csa_013317 [Cucumis sativus])

HSP 1 Score: 946.4 bits (2445), Expect = 1.0e-271
Identity = 474/514 (92.22%), Postives = 483/514 (93.97%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFSSAPVGFH  KTEAVASYCRIS LTKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVASYCRISSLTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAKTCEIHGNG++SN  GVLRSNKK VI+AILQSEREIETTNGIGT GLRGQLNKVV
Sbjct: 61  --LGAKTCEIHGNGLSSNSAGVLRSNKKRVIQAILQSEREIETTNGIGTGGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQG+KELEGLE KARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGMKELEGLENKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYMMS DPEDAPN+PEYVEIGIVGGLPVSVNGE+LSPASLLAKVN
Sbjct: 301 EGDVLEDPANEPKKDMYMMSADPEDAPNKPEYVEIGIVGGLPVSVNGEVLSPASLLAKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF A RELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAAARELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDF 515
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHK+F
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKEF 494

BLAST of CaUC03G053780.1 vs. NCBI nr
Match: XP_008439546.1 (PREDICTED: argininosuccinate synthase, chloroplastic isoform X1 [Cucumis melo] >KAA0055325.1 argininosuccinate synthase [Cucumis melo var. makuwa] >TYJ99250.1 argininosuccinate synthase [Cucumis melo var. makuwa])

HSP 1 Score: 943.7 bits (2438), Expect = 6.5e-271
Identity = 475/514 (92.41%), Postives = 482/514 (93.77%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFSSAPVGFH  KTEAVA YCRIS LTKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVAGYCRISCLTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAKTCEIHGNG++SN  GV RSNKK VI+AILQSEREIETTNGI TRGLRGQLNKVV
Sbjct: 61  --LGAKTCEIHGNGLSSNSSGVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYMMS DPEDAPNEPEYVEIGIVGGLPVSVNGE+LSPASLLAKVN
Sbjct: 301 EGDVLEDPANEPKKDMYMMSADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRS YSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDF 515
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHK+F
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKEF 494

BLAST of CaUC03G053780.1 vs. NCBI nr
Match: XP_023519539.1 (argininosuccinate synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 925.6 bits (2391), Expect = 1.8e-265
Identity = 463/513 (90.25%), Postives = 475/513 (92.59%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFS+AP GFH  K E+VASYCRISG TKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSTAPGGFHPSKAESVASYCRISGPTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAK  EIHGN IA NR  VLRSNKKSVI+AILQSEREIETTNGI TRGLRGQLNKVV
Sbjct: 61  --LGAKASEIHGNSIACNRASVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFV D+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYM+STDPEDAPNEPEYVEIGIVGGLPVS+NGE+LSPASLL KVN
Sbjct: 301 EGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           ++GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EMGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHV SRRS YSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 514
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 493

BLAST of CaUC03G053780.1 vs. NCBI nr
Match: XP_022923631.1 (argininosuccinate synthase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 924.5 bits (2388), Expect = 4.1e-265
Identity = 463/513 (90.25%), Postives = 474/513 (92.40%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGG LFS+AP GFH  K E+VASYCRISG TKLHSFQE                  
Sbjct: 1   MAQLMGGFLFSTAPGGFHPSKAESVASYCRISGPTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAK  EIHGN IA NR  VLRSNKKSVI+AILQSEREIETTNGI TRGLRGQLNKVV
Sbjct: 61  --LGAKASEIHGNSIACNRASVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFV D+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGD+LEDPANEPKKDMYM+STDPEDAPNEPEYVEIGIVGGLPVS+NGE+LSPASLL KVN
Sbjct: 301 EGDILEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHV SRRS YSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 514
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 493

BLAST of CaUC03G053780.1 vs. ExPASy Swiss-Prot
Match: Q9SZX3 (Argininosuccinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g24830 PE=1 SV=3)

HSP 1 Score: 740.0 bits (1909), Expect = 1.9e-212
Identity = 364/453 (80.35%), Postives = 400/453 (88.30%), Query Frame = 0

Query: 60  YQPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKV 119
           +Q L  K  ++ GN + +  V   RS K   IRA+L  +    TT+     GLRG+L KV
Sbjct: 41  FQELSLKRSQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKV 100

Query: 120 VLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLK 179
           VLAYSGGLDTSVIVPWL+ENYGCEVVCFTADVGQG+KELEGLE+KA+ASGA QLVVKDL 
Sbjct: 101 VLAYSGGLDTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLT 160

Query: 180 EEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQ 239
           EEFV DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQ
Sbjct: 161 EEFVKDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQ 220

Query: 240 VRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLS 299
           VRFEL FF+LNP L VVAPWREW+I GREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLS
Sbjct: 221 VRFELTFFSLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLS 280

Query: 300 HEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKV 359
           HEGD+LEDPANEPKKDMYMMS DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LLA++
Sbjct: 281 HEGDLLEDPANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAEL 340

Query: 360 NDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLA 419
           N IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF A +ELESLTLDRE+IQVKD+LA
Sbjct: 341 NTIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAAVQELESLTLDRESIQVKDTLA 400

Query: 420 LKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDI 479
           LKYAE+VYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V  R+S  SLYR+DI
Sbjct: 401 LKYAEMVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDI 460

Query: 480 SSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 513
           SSFE  +IYNQADAAGFIRLYGLP ++RAML K
Sbjct: 461 SSFEGSEIYNQADAAGFIRLYGLPMKIRAMLKK 492

BLAST of CaUC03G053780.1 vs. ExPASy Swiss-Prot
Match: Q3A9W5 (Argininosuccinate synthase OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=argG PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 8.3e-152
Identity = 257/397 (64.74%), Postives = 319/397 (80.35%), Query Frame = 0

Query: 118 KVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKD 177
           KVVLAYSGGLDTS+I+PWL+ENYG EV+   ADVGQG +ELE L +KA  SGA ++ ++D
Sbjct: 4   KVVLAYSGGLDTSIIIPWLKENYGYEVIAMVADVGQG-EELEPLREKAIKSGAAKIYIED 63

Query: 178 LKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGN 237
           +KEEFV DF+FP L+AGA+YE KYLLGTS+ARP+IAK +V++A++ GA AV+HG TGKGN
Sbjct: 64  VKEEFVRDFVFPMLKAGAVYENKYLLGTSVARPLIAKKLVEIAEKEGAVAVAHGATGKGN 123

Query: 238 DQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWH 297
           DQVRFEL   ALNP+L ++APWREW+I  REDAI+YA+K  IPVPVTKK  YS DRNLWH
Sbjct: 124 DQVRFELTVKALNPDLKIIAPWREWEIKSREDAIDYAEKRGIPVPVTKKQPYSMDRNLWH 183

Query: 298 LSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLA 357
           LSHEG +LEDPA EP +D+ +++  PE AP++PEYVEI  V G PV+VNGE LSP  L+ 
Sbjct: 184 LSHEGGILEDPAVEPPEDVLLLTNPPEKAPDQPEYVEIEFVKGEPVAVNGEKLSPVELIF 243

Query: 358 KVNDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDS 417
           K+N++GGKHGIG  DMVENRLVGMKSRGVYETPGGTIL  A RELESLTLDR+T+  K  
Sbjct: 244 KLNELGGKHGIGIADMVENRLVGMKSRGVYETPGGTILTFAHRELESLTLDRQTMHFKQM 303

Query: 418 LALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYRE 477
           +ALKYAEL+Y G WF PLRE+++AF++K  ET TG V +KLYKG+++ A   S YSLY +
Sbjct: 304 VALKYAELIYDGLWFTPLREALEAFVDKTQETVTGKVRVKLYKGNIYPAGITSPYSLYVK 363

Query: 478 DISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDF 515
           D+++F    +YNQ DA GFI L+GLP +VRAM  K +
Sbjct: 364 DLATFGEDNLYNQKDAEGFINLFGLPLKVRAMTQKPY 399

BLAST of CaUC03G053780.1 vs. ExPASy Swiss-Prot
Match: B8FWX2 (Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain DSM 10664 / DCB-2) OX=272564 GN=argG PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.6e-147
Identity = 249/397 (62.72%), Postives = 313/397 (78.84%), Query Frame = 0

Query: 116 LNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVV 175
           + KVVLAYSGGLDTS+I+PWL+ENYG EV+   AD+GQG +ELE L +KA  SGA +L +
Sbjct: 1   MKKVVLAYSGGLDTSIIIPWLKENYGYEVIAMAADLGQG-EELEPLHEKAIKSGASKLYI 60

Query: 176 KDLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGK 235
           +DL+EEFV+DFI+P L+AGA+YE KYLLGTS ARP+IA+ +V++A + GA A++HG TGK
Sbjct: 61  EDLQEEFVTDFIYPTLKAGAVYEGKYLLGTSFARPLIAQRLVEIAAKEGAVAIAHGATGK 120

Query: 236 GNDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNL 295
           GNDQVRFELA  ALNP+L ++APWR WDI  REDAI+YA +  IPVPVTK   YS DRN+
Sbjct: 121 GNDQVRFELAVKALNPDLEIIAPWRIWDIKSREDAIDYAVERGIPVPVTKDRPYSMDRNV 180

Query: 296 WHLSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASL 355
           WHLSHEG  LEDP NEPKKD+Y++   PEDAP+E EY+E+    G+PVS+NGE L P  L
Sbjct: 181 WHLSHEGGDLEDPWNEPKKDLYLLGVSPEDAPDEAEYLELDFEQGIPVSLNGEKLGPVQL 240

Query: 356 LAKVNDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVK 415
           L  +N++GGKHGIG +DMVENRLVGMKSRGVYETPGGTIL+TA + LE LTLDR T+  K
Sbjct: 241 LETLNEVGGKHGIGIVDMVENRLVGMKSRGVYETPGGTILYTAHQALEHLTLDRLTLHYK 300

Query: 416 DSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLY 475
           + +ALKYAELVY G W  PLRE++DAF++   +  TG+V LKLYKG+  +A  +S YSLY
Sbjct: 301 EQIALKYAELVYDGVWHSPLREALDAFVDVTQKNVTGTVRLKLYKGNCSLAGVKSPYSLY 360

Query: 476 REDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 513
            E+ ++F    +YNQ DA GFI L+GLP +VRA++ K
Sbjct: 361 SEEFATFGRDGVYNQKDAEGFINLFGLPLKVRALMEK 396

BLAST of CaUC03G053780.1 vs. ExPASy Swiss-Prot
Match: Q24ZG8 (Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=argG PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.6e-147
Identity = 249/397 (62.72%), Postives = 313/397 (78.84%), Query Frame = 0

Query: 116 LNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVV 175
           + KVVLAYSGGLDTS+I+PWL+ENYG EV+   AD+GQG +ELE L +KA  SGA +L +
Sbjct: 9   MKKVVLAYSGGLDTSIIIPWLKENYGYEVIAMAADLGQG-EELEPLHEKAIKSGASKLYI 68

Query: 176 KDLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGK 235
           +DL+EEFV+DFI+P L+AGA+YE KYLLGTS ARP+IA+ +V++A + GA A++HG TGK
Sbjct: 69  EDLQEEFVTDFIYPTLKAGAVYEGKYLLGTSFARPLIAQRLVEIAAKEGAVAIAHGATGK 128

Query: 236 GNDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNL 295
           GNDQVRFELA  ALNP+L ++APWR WDI  REDAI+YA +  IPVPVTK   YS DRN+
Sbjct: 129 GNDQVRFELAVKALNPDLEIIAPWRIWDIKSREDAIDYAVERGIPVPVTKDRPYSMDRNV 188

Query: 296 WHLSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASL 355
           WHLSHEG  LEDP NEPKKD+Y++   PEDAP+E EY+E+    G+PVS+NGE L P  L
Sbjct: 189 WHLSHEGGDLEDPWNEPKKDLYLLGVSPEDAPDEAEYLELDFEQGIPVSLNGEKLGPVQL 248

Query: 356 LAKVNDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVK 415
           L  +N++GGKHGIG +DMVENRLVGMKSRGVYETPGGTIL+TA + LE LTLDR T+  K
Sbjct: 249 LETLNEVGGKHGIGIVDMVENRLVGMKSRGVYETPGGTILYTAHQALEHLTLDRLTLHYK 308

Query: 416 DSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLY 475
           + +ALKYAELVY G W  PLRE++DAF++   +  TG+V LKLYKG+  +A  +S YSLY
Sbjct: 309 EQIALKYAELVYDGVWHSPLREALDAFVDVTQKNVTGTVRLKLYKGNCSLAGVKSPYSLY 368

Query: 476 REDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 513
            E+ ++F    +YNQ DA GFI L+GLP +VRA++ K
Sbjct: 369 SEEFATFGRDGVYNQKDAEGFINLFGLPLKVRALMEK 404

BLAST of CaUC03G053780.1 vs. ExPASy Swiss-Prot
Match: A4J173 (Argininosuccinate synthase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=argG PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 8.0e-147
Identity = 254/395 (64.30%), Postives = 313/395 (79.24%), Query Frame = 0

Query: 118 KVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKD 177
           KVVLAYSGGLDTSVI+ WL+ENYG EV+  TAD+GQG +EL  LE+KA  SGA ++ ++D
Sbjct: 3   KVVLAYSGGLDTSVIIAWLKENYGYEVIAVTADLGQG-EELAPLEEKALQSGASKIYIED 62

Query: 178 LKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGN 237
           L++EFV D+I+P L+AGAIYE KYLLGTS ARP+IAK +V++A++ GA AV+HG TGKGN
Sbjct: 63  LRKEFVEDYIWPTLKAGAIYEGKYLLGTSFARPLIAKKLVEIAEKEGAVAVAHGATGKGN 122

Query: 238 DQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWH 297
           DQVRFEL   AL P+L V+APWREWDI  REDAI+YA+   IPVPVTKKSIYSRDRN+WH
Sbjct: 123 DQVRFELGVKALAPHLKVIAPWREWDIRSREDAIDYAEARGIPVPVTKKSIYSRDRNIWH 182

Query: 298 LSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLA 357
           +SHEG  LE PAN    DM M++  PE AP++P YVEIG   G+PVS+NGE++    LL 
Sbjct: 183 ISHEGGELESPANAASYDMLMLTVPPEKAPDQPTYVEIGFEKGIPVSINGELMDSIGLLE 242

Query: 358 KVNDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDS 417
           K+N IGG++GIG +DMVENRLVGMKSRGVYETPGG IL  A RELE LTLDR T+  K+ 
Sbjct: 243 KLNVIGGENGIGIVDMVENRLVGMKSRGVYETPGGAILVYAHRELELLTLDRATLHYKEQ 302

Query: 418 LALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYRE 477
           +AL+YAELVY G WF PLRE++DAF++    T TG+V LKLYKG++  A   S YSLY E
Sbjct: 303 IALRYAELVYDGVWFAPLREALDAFVDNTQRTVTGTVKLKLYKGNMMPAGVTSPYSLYDE 362

Query: 478 DISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 513
           ++S+F   ++YNQADAAGFI L+GLP +VRAM+ K
Sbjct: 363 ELSTFGRDEVYNQADAAGFINLFGLPLKVRAMMEK 396

BLAST of CaUC03G053780.1 vs. ExPASy TrEMBL
Match: A0A0A0LWM9 (Citrulline--aspartate ligase OS=Cucumis sativus OX=3659 GN=Csa_1G050400 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 4.9e-272
Identity = 474/514 (92.22%), Postives = 483/514 (93.97%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFSSAPVGFH  KTEAVASYCRIS LTKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVASYCRISSLTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAKTCEIHGNG++SN  GVLRSNKK VI+AILQSEREIETTNGIGT GLRGQLNKVV
Sbjct: 61  --LGAKTCEIHGNGLSSNSAGVLRSNKKRVIQAILQSEREIETTNGIGTGGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQG+KELEGLE KARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGMKELEGLENKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYMMS DPEDAPN+PEYVEIGIVGGLPVSVNGE+LSPASLLAKVN
Sbjct: 301 EGDVLEDPANEPKKDMYMMSADPEDAPNKPEYVEIGIVGGLPVSVNGEVLSPASLLAKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF A RELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAAARELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDF 515
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHK+F
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKEF 494

BLAST of CaUC03G053780.1 vs. ExPASy TrEMBL
Match: A0A5D3BHJ5 (Citrulline--aspartate ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004610 PE=3 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 3.1e-271
Identity = 475/514 (92.41%), Postives = 482/514 (93.77%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFSSAPVGFH  KTEAVA YCRIS LTKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVAGYCRISCLTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAKTCEIHGNG++SN  GV RSNKK VI+AILQSEREIETTNGI TRGLRGQLNKVV
Sbjct: 61  --LGAKTCEIHGNGLSSNSSGVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYMMS DPEDAPNEPEYVEIGIVGGLPVSVNGE+LSPASLLAKVN
Sbjct: 301 EGDVLEDPANEPKKDMYMMSADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRS YSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDF 515
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHK+F
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKEF 494

BLAST of CaUC03G053780.1 vs. ExPASy TrEMBL
Match: A0A1S3AZP1 (Citrulline--aspartate ligase OS=Cucumis melo OX=3656 GN=LOC103484309 PE=3 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 3.1e-271
Identity = 475/514 (92.41%), Postives = 482/514 (93.77%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFSSAPVGFH  KTEAVA YCRIS LTKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVAGYCRISCLTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAKTCEIHGNG++SN  GV RSNKK VI+AILQSEREIETTNGI TRGLRGQLNKVV
Sbjct: 61  --LGAKTCEIHGNGLSSNSSGVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYMMS DPEDAPNEPEYVEIGIVGGLPVSVNGE+LSPASLLAKVN
Sbjct: 301 EGDVLEDPANEPKKDMYMMSADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRS YSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDF 515
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHK+F
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKEF 494

BLAST of CaUC03G053780.1 vs. ExPASy TrEMBL
Match: A0A6J1E7A9 (Citrulline--aspartate ligase OS=Cucurbita moschata OX=3662 GN=LOC111431281 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.0e-265
Identity = 463/513 (90.25%), Postives = 474/513 (92.40%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGG LFS+AP GFH  K E+VASYCRISG TKLHSFQE                  
Sbjct: 1   MAQLMGGFLFSTAPGGFHPSKAESVASYCRISGPTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAK  EIHGN IA NR  VLRSNKKSVI+AILQSEREIETTNGI TRGLRGQLNKVV
Sbjct: 61  --LGAKASEIHGNSIACNRASVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFV D+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGD+LEDPANEPKKDMYM+STDPEDAPNEPEYVEIGIVGGLPVS+NGE+LSPASLL KVN
Sbjct: 301 EGDILEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           +IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHV SRRS YSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 514
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 493

BLAST of CaUC03G053780.1 vs. ExPASy TrEMBL
Match: A0A6J1KF74 (Citrulline--aspartate ligase OS=Cucurbita maxima OX=3661 GN=LOC111495262 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 8.3e-264
Identity = 459/513 (89.47%), Postives = 473/513 (92.20%), Query Frame = 0

Query: 1   MAQIMGGSLFSSAPVGFHQPKTEAVASYCRISGLTKLHSFQEAFASIILSFLSLNDFYPY 60
           MAQ+MGGSLFS+AP GFH  K E+VASYCRISG TKLHSFQE                  
Sbjct: 1   MAQLMGGSLFSTAPGGFHPSKAESVASYCRISGSTKLHSFQE------------------ 60

Query: 61  QPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKVV 120
             LGAK  EIHGN IA N   VLRSNKKSVI+AIL SERE+ETTNGI TRGLRGQLNKVV
Sbjct: 61  --LGAKASEIHGNSIACNMASVLRSNKKSVIQAILPSERELETTNGITTRGLRGQLNKVV 120

Query: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKE 180
           LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKE
Sbjct: 121 LAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKE 180

Query: 181 EFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240
           EFV D+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV
Sbjct: 181 EFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQV 240

Query: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSH 300
           RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSH
Sbjct: 241 RFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSH 300

Query: 301 EGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKVN 360
           EGDVLEDPANEPKKDMYM+STDPEDAPNEPEYVEIGIVGGLPVS+NGE+LSPASLL KVN
Sbjct: 301 EGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVN 360

Query: 361 DIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLAL 420
           ++GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF ATRELESLTLDRETIQVKDSLAL
Sbjct: 361 EMGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLAL 420

Query: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDIS 480
           KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHV SR+S YSLYREDIS
Sbjct: 421 KYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRQSQYSLYREDIS 480

Query: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 514
           SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD
Sbjct: 481 SFENGQIYNQADAAGFIRLYGLPTRVRAMLHKD 493

BLAST of CaUC03G053780.1 vs. TAIR 10
Match: AT4G24830.1 (arginosuccinate synthase family )

HSP 1 Score: 740.0 bits (1909), Expect = 1.3e-213
Identity = 364/453 (80.35%), Postives = 400/453 (88.30%), Query Frame = 0

Query: 60  YQPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKV 119
           +Q L  K  ++ GN + +  V   RS K   IRA+L  +    TT+     GLRG+L KV
Sbjct: 41  FQELSLKRSQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKV 100

Query: 120 VLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLK 179
           VLAYSGGLDTSVIVPWL+ENYGCEVVCFTADVGQG+KELEGLE+KA+ASGA QLVVKDL 
Sbjct: 101 VLAYSGGLDTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLT 160

Query: 180 EEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQ 239
           EEFV DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQ
Sbjct: 161 EEFVKDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQ 220

Query: 240 VRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLS 299
           VRFEL FF+LNP L VVAPWREW+I GREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLS
Sbjct: 221 VRFELTFFSLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLS 280

Query: 300 HEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKV 359
           HEGD+LEDPANEPKKDMYMMS DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LLA++
Sbjct: 281 HEGDLLEDPANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAEL 340

Query: 360 NDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLA 419
           N IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILF A +ELESLTLDRE+IQVKD+LA
Sbjct: 341 NTIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAAVQELESLTLDRESIQVKDTLA 400

Query: 420 LKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDI 479
           LKYAE+VYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V  R+S  SLYR+DI
Sbjct: 401 LKYAEMVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDI 460

Query: 480 SSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 513
           SSFE  +IYNQADAAGFIRLYGLP ++RAML K
Sbjct: 461 SSFEGSEIYNQADAAGFIRLYGLPMKIRAMLKK 492

BLAST of CaUC03G053780.1 vs. TAIR 10
Match: AT4G24830.2 (arginosuccinate synthase family )

HSP 1 Score: 644.0 bits (1660), Expect = 9.9e-185
Identity = 326/453 (71.96%), Postives = 358/453 (79.03%), Query Frame = 0

Query: 60  YQPLGAKTCEIHGNGIASNRVGVLRSNKKSVIRAILQSEREIETTNGIGTRGLRGQLNKV 119
           +Q L  K  ++ GN + +  V   RS K   IRA+L  +    TT+     GLRG+L KV
Sbjct: 41  FQELSLKRSQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKV 100

Query: 120 VLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLK 179
           VLAYSGGLDTSVIVPWL+ENYGCEVVCFTADVGQG+KELEGLE+KA+ASGA QLVVKDL 
Sbjct: 101 VLAYSGGLDTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLT 160

Query: 180 EEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQ 239
           EEFV DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQ
Sbjct: 161 EEFVKDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQ 220

Query: 240 VRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLS 299
           VRFEL FF+LNP L VVAPWREW+I GREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLS
Sbjct: 221 VRFELTFFSLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLS 280

Query: 300 HEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEILSPASLLAKV 359
           HEGD+LEDPANEPKKDMYMMS DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LLA++
Sbjct: 281 HEGDLLEDPANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAEL 340

Query: 360 NDIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFTATRELESLTLDRETIQVKDSLA 419
           N IGGKHGIGRIDMVENRLVGMKSR                                   
Sbjct: 341 NTIGGKHGIGRIDMVENRLVGMKSR----------------------------------- 400

Query: 420 LKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDI 479
                    GRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V  R+S  SLYR+DI
Sbjct: 401 ---------GRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDI 448

Query: 480 SSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 513
           SSFE  +IYNQADAAGFIRLYGLP ++RAML K
Sbjct: 461 SSFEGSEIYNQADAAGFIRLYGLPMKIRAMLKK 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895641.13.4e-27292.79argininosuccinate synthase, chloroplastic-like [Benincasa hispida][more]
XP_004152522.11.0e-27192.22argininosuccinate synthase, chloroplastic [Cucumis sativus] >KGN64401.1 hypothet... [more]
XP_008439546.16.5e-27192.41PREDICTED: argininosuccinate synthase, chloroplastic isoform X1 [Cucumis melo] >... [more]
XP_023519539.11.8e-26590.25argininosuccinate synthase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022923631.14.1e-26590.25argininosuccinate synthase, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SZX31.9e-21280.35Argininosuccinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
Q3A9W58.3e-15264.74Argininosuccinate synthase OS=Carboxydothermus hydrogenoformans (strain ATCC BAA... [more]
B8FWX23.6e-14762.72Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain DSM 10664 / D... [more]
Q24ZG83.6e-14762.72Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain Y51) OX=13811... [more]
A4J1738.0e-14764.30Argininosuccinate synthase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LWM94.9e-27292.22Citrulline--aspartate ligase OS=Cucumis sativus OX=3659 GN=Csa_1G050400 PE=3 SV=... [more]
A0A5D3BHJ53.1e-27192.41Citrulline--aspartate ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AZP13.1e-27192.41Citrulline--aspartate ligase OS=Cucumis melo OX=3656 GN=LOC103484309 PE=3 SV=1[more]
A0A6J1E7A92.0e-26590.25Citrulline--aspartate ligase OS=Cucurbita moschata OX=3662 GN=LOC111431281 PE=3 ... [more]
A0A6J1KF748.3e-26489.47Citrulline--aspartate ligase OS=Cucurbita maxima OX=3661 GN=LOC111495262 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G24830.11.3e-21380.35arginosuccinate synthase family [more]
AT4G24830.29.9e-18571.96arginosuccinate synthase family [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001518Argininosuccinate synthasePFAMPF00764Arginosuc_synthcoord: 120..506
e-value: 2.6E-158
score: 527.4
IPR001518Argininosuccinate synthaseTIGRFAMTIGR00032TIGR00032coord: 118..508
e-value: 7.6E-150
score: 497.5
IPR001518Argininosuccinate synthasePANTHERPTHR11587ARGININOSUCCINATE SYNTHASEcoord: 115..512
IPR024074Argininosuccinate synthetase, catalytic/multimerisation domain bodyGENE3D3.90.1260.10Argininosuccinate synthetase, chain A, domain 2coord: 178..468
e-value: 6.6E-164
score: 547.0
IPR024074Argininosuccinate synthetase, catalytic/multimerisation domain bodySUPERFAMILY69864Argininosuccinate synthetase, C-terminal domaincoord: 286..513
NoneNo IPR availableGENE3D1.20.5.470Single helix bincoord: 488..513
e-value: 1.1E-8
score: 36.3
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 117..281
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 118..287
e-value: 6.6E-164
score: 547.0
IPR018223Argininosuccinate synthase, conserved sitePROSITEPS00564ARGININOSUCCIN_SYN_1coord: 122..130
IPR018223Argininosuccinate synthase, conserved sitePROSITEPS00565ARGININOSUCCIN_SYN_2coord: 231..242
IPR023434Argininosuccinate synthase, type 1 subfamilyHAMAPMF_00005Arg_succ_synth_type1coord: 116..512
score: 53.722038
IPR023434Argininosuccinate synthase, type 1 subfamilyCDDcd01999Argininosuccinate_Synthasecoord: 119..503
e-value: 0.0
score: 665.388

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CaUC03G053780CaUC03G053780gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4288454..4289178exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4289454..4289542exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4289735..4289920exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4290008..4290082exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4290345..4290527exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4290684..4290732exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4291019..4291160exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4291477..4291558exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4291606..4291724exon
CaUC03G053780.1-exonCaUC03G053780.1-exon-Ciama_Chr03:4291985..4292170exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CaUC03G053780.1-three_prime_utrCaUC03G053780.1-three_prime_utr-Ciama_Chr03:4288454..4288625three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4288626..4289178CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4289454..4289542CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4289735..4289920CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4290008..4290082CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4290345..4290527CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4290684..4290732CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4291019..4291160CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4291477..4291558CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4291606..4291724CDS
CaUC03G053780.1-cdsCaUC03G053780.1-cds-Ciama_Chr03:4291985..4292051CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CaUC03G053780.1-five_prime_utrCaUC03G053780.1-five_prime_utr-Ciama_Chr03:4292052..4292170five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CaUC03G053780.1CaUC03G053780.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006526 arginine biosynthetic process
biological_process GO:0000053 argininosuccinate metabolic process
biological_process GO:0000050 urea cycle
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004055 argininosuccinate synthase activity
molecular_function GO:0005524 ATP binding