CSPI07G17790.1 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI07G17790.1
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectinesterase
LocationChr7: 16016696 .. 16019408 (-)
Sequence length2090
RNA-Seq ExpressionCSPI07G17790.1
SyntenyCSPI07G17790.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAAATTAAAAACTACTAATTTCTCAATAATAATAATAACAATAAAACGAAGTAGGATGGATCTTCATCCACTCACCTCTTCAACACCCATATCCAACTCTCCCCCTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCTGTTTCAGACATGTACGGCCTGAGAAACAGAGCTCAAATCTCTTCAAACCCCACCTCCATTTCCACCATTTCCCCTTTCTTCTTCATCTTCTTCCTCTTCTCCTTCAAACCCATTTCTTCAAATCCCCAAGTCCACCAAACTGCTCGATCCCTTTGCAATGGCACTCTCTTCCCAGACCTCTGTTTCTCTAAACTCTCTTCTTTTCCCCAATTGGCTTCCCTCTCCCCGGAAAAGCTCGCCGGCAGTGCCCTCAACTTCACTTCCCGTGAGGTCCTCCTTGCCTACACCAATTGCACCAACCTCAAGACTCACCTTTACAGTGGCCTAAATCCCACCGACCGCCACGCCCTCGACGACTGTCTTGAGCTTCTCGACGGCAGCATTGCAGAGCTCAAAGCTTCGATCTTTGATCTGGCGCCGTCGCAATCCCCTGCTCTTCACTCCCACGATTTGTTGACATTGGTCAGCGCCGCCATGACCAACCACCGAACTTGTGTTGATGGGTTCTATAATAGTTCGGGAACTGTGAGGAGCAGGGTGGAACTTTATTTGGGGAAAATCGGACAACATTTAAGTATTGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTACGGGGGTAGATCAGGAGGTGTTGCCGGAGTATGGGGCAGTCAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCAGTGAATGAGACGAAGTTCAATATGGTAGTGGCGAAGGATGGGTCCGGGAATTTCACGACTGTGAGCGAGGCCGTGGCCGCCGCTCCGAATGCGAGCTCCACCAGGTTTTCTTGAACTGAAGTAATTAAATTCATAATCATAAGCTTTGAAAAATGATAATTAAGAGAAAAAGTGGATTTGTTATGGGACAGGTTTGTAATATACATAAAAGCAGGGGCGTATTTTGAGAACGTGGAAATAGGAAGGGCAAAGTCAAACTTGATGTTCGTCGGCGACGGAATCGGAAAGACGCTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACTTTCCGATCAGCCACCGTCGGTCAGTTCTTTTTTTAACATTCTTATTGAATTTATGAAACCACCTGAGTATGATCAGATCATGGCTCTCAGATGATTTTATTTAAGTTGGTAGTTTAATTAGTTTTTAATAATACAATCTTCAAAAGATTCTAAAAATTTTGGATCTGTTTTAATTTTTTTTAGATTTCAAAAACATTTCTTTTTAAGTTTTAAAATGTAGGTTTAGGATGTCAACGTGAGTTGTTGGGCTCATGTGACAAGTGTATTTAGTAAGGACAAAAGGACTTGGATTCATTTTCCTCCATCCAAGTTTTTACGCTACCTTTTTACGAAAAGGTTAAGTTCCTATTTTAAATAATTATATGACATGATCTCTTTTTCTCTTAATTTTTTAAAAATTTTAAATATCATATAACTATTTCAAAAGTTAAAAAATAAATACTTTGACAATCTTTTAAACACTCTTTTTAACTTAGGAATGAAAAAGATAATTTTTCATTGTTCTTCAAAATTTATTCACTATTAGCAATAACACAGGTTTTGTTTATTATGTAGCGGTGGTTGGCCCTGGGTTCATAGCAAAAGGCATAACATTTGAGAATTATGCGGGTCCAAGCAAGCACCAAGCTGTAGCCTTACGAAGCAACTCTGACTTCTCAGCATTTTACCAATGTAGCTTCATTGGCTATCAAGACACTCTCTACGTTCACTCCCTTCGTCAATTCTACCGCGAATGTGATGTCTATGGTACCATTGATTTCATTTTTGGTAACGCTGCCGTTGTTTTCCAAAATTGCAACCTTTATGCTCGGAAACCCAATTCAAATCAACGCAACATATTCACTGCTCAAGGTCGCGAAGACCCCAATCAAAACACTGGAATCTCAATCCTCAACTGTAAGGTTGAAGCTGCCTCTGACCTAATCCCAGTTCTTTCCTCGTTTAGAACTTACTTGGGTCGTCCTTGGAAGTTGTACTCGAGGACAGTTTTTCTCCGATCGTTTATCGGACAATTGATTGAACCGGTTGGATGGCTTGAGTGGAATGGGACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTAAATAGGGGGCCTGGTTCAAATACAACCATGCGAGTAACTTGGCCGGGTTATCGAGTTATTACTAATGCAACAGAAGCGAGTCAGTTTACGGTTGAGGGATTTATACAAGGTAGCTCGTGGTTGAACTCGACCGAGATTCCTTTCTTCTCCGGTTTGACTCCACCTTAGATTGAAGGGAAGGATCAAATTTTAGGGTTATCTTTGGGTTTTTATTATTATTATTTTTTTGTAAGGACTAAGATGCTTTTGATGTTGAAAGAGGGAGAGAGTAATGGCTGATCCATGGTCCACAAAAGGACTTCACATGTGATCATATGATATCATTGGTAATTCCCGTTTTAATCAAATATTATATACTAAATTTGAAGTAGAGCATCTAATCTAATTAATATGTTATTTTAGATT

mRNA sequence

TTAAAATTAAAAACTACTAATTTCTCAATAATAATAATAACAATAAAACGAAGTAGGATGGATCTTCATCCACTCACCTCTTCAACACCCATATCCAACTCTCCCCCTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCTGTTTCAGACATGTACGGCCTGAGAAACAGAGCTCAAATCTCTTCAAACCCCACCTCCATTTCCACCATTTCCCCTTTCTTCTTCATCTTCTTCCTCTTCTCCTTCAAACCCATTTCTTCAAATCCCCAAGTCCACCAAACTGCTCGATCCCTTTGCAATGGCACTCTCTTCCCAGACCTCTGTTTCTCTAAACTCTCTTCTTTTCCCCAATTGGCTTCCCTCTCCCCGGAAAAGCTCGCCGGCAGTGCCCTCAACTTCACTTCCCGTGAGGTCCTCCTTGCCTACACCAATTGCACCAACCTCAAGACTCACCTTTACAGTGGCCTAAATCCCACCGACCGCCACGCCCTCGACGACTGTCTTGAGCTTCTCGACGGCAGCATTGCAGAGCTCAAAGCTTCGATCTTTGATCTGGCGCCGTCGCAATCCCCTGCTCTTCACTCCCACGATTTGTTGACATTGGTCAGCGCCGCCATGACCAACCACCGAACTTGTGTTGATGGGTTCTATAATAGTTCGGGAACTGTGAGGAGCAGGGTGGAACTTTATTTGGGGAAAATCGGACAACATTTAAGTATTGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTACGGGGGTAGATCAGGAGGTGTTGCCGGAGTATGGGGCAGTCAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCAGTGAATGAGACGAAGTTCAATATGGTAGTGGCGAAGGATGGGTCCGGGAATTTCACGACTGTGAGCGAGGCCGTGGCCGCCGCTCCGAATGCGAGCTCCACCAGGTTTGTAATATACATAAAAGCAGGGGCGTATTTTGAGAACGTGGAAATAGGAAGGGCAAAGTCAAACTTGATGTTCGTCGGCGACGGAATCGGAAAGACGCTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACTTTCCGATCAGCCACCGTCGCGGTGGTTGGCCCTGGGTTCATAGCAAAAGGCATAACATTTGAGAATTATGCGGGTCCAAGCAAGCACCAAGCTGTAGCCTTACGAAGCAACTCTGACTTCTCAGCATTTTACCAATGTAGCTTCATTGGCTATCAAGACACTCTCTACGTTCACTCCCTTCGTCAATTCTACCGCGAATGTGATGTCTATGGTACCATTGATTTCATTTTTGGTAACGCTGCCGTTGTTTTCCAAAATTGCAACCTTTATGCTCGGAAACCCAATTCAAATCAACGCAACATATTCACTGCTCAAGGTCGCGAAGACCCCAATCAAAACACTGGAATCTCAATCCTCAACTGTAAGGTTGAAGCTGCCTCTGACCTAATCCCAGTTCTTTCCTCGTTTAGAACTTACTTGGGTCGTCCTTGGAAGTTGTACTCGAGGACAGTTTTTCTCCGATCGTTTATCGGACAATTGATTGAACCGGTTGGATGGCTTGAGTGGAATGGGACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTAAATAGGGGGCCTGGTTCAAATACAACCATGCGAGTAACTTGGCCGGGTTATCGAGTTATTACTAATGCAACAGAAGCGAGTCAGTTTACGGTTGAGGGATTTATACAAGGTAGCTCGTGGTTGAACTCGACCGAGATTCCTTTCTTCTCCGGTTTGACTCCACCTTAGATTGAAGGGAAGGATCAAATTTTAGGGTTATCTTTGGGTTTTTATTATTATTATTTTTTTGTAAGGACTAAGATGCTTTTGATGTTGAAAGAGGGAGAGAGTAATGGCTGATCCATGGTCCACAAAAGGACTTCACATGTGATCATATGATATCATTGGTAATTCCCGTTTTAATCAAATATTATATACTAAATTTGAAGTAGAGCATCTAATCTAATTAATATGTTATTTTAGATT

Coding sequence (CDS)

ATGTACGGCCTGAGAAACAGAGCTCAAATCTCTTCAAACCCCACCTCCATTTCCACCATTTCCCCTTTCTTCTTCATCTTCTTCCTCTTCTCCTTCAAACCCATTTCTTCAAATCCCCAAGTCCACCAAACTGCTCGATCCCTTTGCAATGGCACTCTCTTCCCAGACCTCTGTTTCTCTAAACTCTCTTCTTTTCCCCAATTGGCTTCCCTCTCCCCGGAAAAGCTCGCCGGCAGTGCCCTCAACTTCACTTCCCGTGAGGTCCTCCTTGCCTACACCAATTGCACCAACCTCAAGACTCACCTTTACAGTGGCCTAAATCCCACCGACCGCCACGCCCTCGACGACTGTCTTGAGCTTCTCGACGGCAGCATTGCAGAGCTCAAAGCTTCGATCTTTGATCTGGCGCCGTCGCAATCCCCTGCTCTTCACTCCCACGATTTGTTGACATTGGTCAGCGCCGCCATGACCAACCACCGAACTTGTGTTGATGGGTTCTATAATAGTTCGGGAACTGTGAGGAGCAGGGTGGAACTTTATTTGGGGAAAATCGGACAACATTTAAGTATTGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTACGGGGGTAGATCAGGAGGTGTTGCCGGAGTATGGGGCAGTCAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCAGTGAATGAGACGAAGTTCAATATGGTAGTGGCGAAGGATGGGTCCGGGAATTTCACGACTGTGAGCGAGGCCGTGGCCGCCGCTCCGAATGCGAGCTCCACCAGGTTTGTAATATACATAAAAGCAGGGGCGTATTTTGAGAACGTGGAAATAGGAAGGGCAAAGTCAAACTTGATGTTCGTCGGCGACGGAATCGGAAAGACGCTGATAAAGGCCGACCGGAATGTCGTCGACGGCTGGACTACTTTCCGATCAGCCACCGTCGCGGTGGTTGGCCCTGGGTTCATAGCAAAAGGCATAACATTTGAGAATTATGCGGGTCCAAGCAAGCACCAAGCTGTAGCCTTACGAAGCAACTCTGACTTCTCAGCATTTTACCAATGTAGCTTCATTGGCTATCAAGACACTCTCTACGTTCACTCCCTTCGTCAATTCTACCGCGAATGTGATGTCTATGGTACCATTGATTTCATTTTTGGTAACGCTGCCGTTGTTTTCCAAAATTGCAACCTTTATGCTCGGAAACCCAATTCAAATCAACGCAACATATTCACTGCTCAAGGTCGCGAAGACCCCAATCAAAACACTGGAATCTCAATCCTCAACTGTAAGGTTGAAGCTGCCTCTGACCTAATCCCAGTTCTTTCCTCGTTTAGAACTTACTTGGGTCGTCCTTGGAAGTTGTACTCGAGGACAGTTTTTCTCCGATCGTTTATCGGACAATTGATTGAACCGGTTGGATGGCTTGAGTGGAATGGGACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTAAATAGGGGGCCTGGTTCAAATACAACCATGCGAGTAACTTGGCCGGGTTATCGAGTTATTACTAATGCAACAGAAGCGAGTCAGTTTACGGTTGAGGGATTTATACAAGGTAGCTCGTGGTTGAACTCGACCGAGATTCCTTTCTTCTCCGGTTTGACTCCACCTTAG

Protein sequence

MYGLRNRAQISSNPTSISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTEIPFFSGLTPP*
Homology
BLAST of CSPI07G17790.1 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 6.9e-152
Identity = 276/489 (56.44%), Postives = 353/489 (72.19%), Query Frame = 0

Query: 75  KLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFD 134
           +L  +ALN T   V L+ +N ++L   L S L+  D  A DDCLELLD ++ +L  +I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 135 LAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGT------------VRSRVELYLG 194
           L  S SP L  H++  L+SAAMTN RTC+DGF +S               V   ++  L 
Sbjct: 99  LR-SHSPEL--HNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 195 KIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETK 254
            I  H+S  LAML+ IPG +    V ++V         GFP WVS  DR LLQ  V+ETK
Sbjct: 159 NISSHVSDSLAMLENIPG-HIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETK 218

Query: 255 FNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGI 314
            N+VVA++G+GN+TT+ EA++AAPN+S TRFVIYIK G YFEN+EI R K+ +MF+GDGI
Sbjct: 219 VNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGI 278

Query: 315 GKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAF 374
           G+T+IKA+R+  DGWT F SATV V G GFIAK ++F NYAGP KHQAVALRS+SD SA+
Sbjct: 279 GRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAY 338

Query: 375 YQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFT 434
           Y+CSF  YQDT+YVHS +QFYRECD+YGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+T
Sbjct: 339 YRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYT 398

Query: 435 AQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEP 494
           AQGRE+  + TGISI++ ++ AA DLIPV ++F+ YLGRPW+LYSRTV ++SFI  L++P
Sbjct: 399 AQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDP 458

Query: 495 VGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSS 552
            GWL+W   FAL+TLYYGEY+N GPGSN T RV WPG++ I    EASQF+V  FI G+ 
Sbjct: 459 AGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNK 514

BLAST of CSPI07G17790.1 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 2.0e-143
Identity = 263/482 (54.56%), Postives = 337/482 (69.92%), Query Frame = 0

Query: 81  LNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQS 140
           LN T  +V LA +N ++L+T L+  L   +R A +DCL LLD +I++L+ ++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 141 PALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSIDL 200
            +L  +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 201 AMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGS 260
            ML+ I          ++  P+   V   +P W+S  D+RLL+A V ET +N+ VA DG+
Sbjct: 181 HMLQVIS--------RKKPSPKSSEVDVEYPSWLSENDQRLLEAPVQETNYNLSVAIDGT 240

Query: 261 GNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRN 320
           GNFTT+++AV AAPN S TRF+IYIK G YFENVE+ + K+ +MF+GDGIGKT+IKA+R+
Sbjct: 241 GNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRS 300

Query: 321 VVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQD 380
            +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQD
Sbjct: 301 RIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQD 360

Query: 381 TLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQN 440
           TLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q 
Sbjct: 361 TLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQP 420

Query: 441 TGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTF 500
           TGISILNC++ AA DLIPV  +F+ YLGRPW+ YSRTV ++SFI  LI P GWLE    F
Sbjct: 421 TGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDF 480

Query: 501 ALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTEIPFF 555
           AL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI GS+WLNST IPF 
Sbjct: 481 ALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFS 531

BLAST of CSPI07G17790.1 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.2e-142
Identity = 246/320 (76.88%), Postives = 272/320 (85.00%), Query Frame = 0

Query: 237 AVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLM 296
           AV +   ++VVAKDGSGNFTTV  AVAAA ++S+ RFVIYIK GAYFE V++ + K+NLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 297 FVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSN 356
           F+GDGIGKT IK +R+VVDGWTTFRS+TVAVVG GFIA+GI+FENYAGPSKHQAVALRS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 357 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSN 416
           +DFSAFYQCSF+GYQDTLYVHSLRQFY ECDVYGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 417 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFI 476
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DLIPVLSSF+TYLGRPWK YSRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 477 GQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEG 536
             LI+P GWLEW+G FAL TLYY EY N GPGSNTT RVTWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 537 FIQGSSWLNSTEIPFFSGLT 557
           FIQGS WL S  IP +  LT
Sbjct: 302 FIQGSQWLTSYNIPVYLNLT 321

BLAST of CSPI07G17790.1 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 492.7 bits (1267), Expect = 5.7e-138
Identity = 269/528 (50.95%), Postives = 344/528 (65.15%), Query Frame = 0

Query: 42  HQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTH 101
           H   +S+C+ TL+P+LCFS +++       S +++  ++LN T++ V   Y     L   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 118

Query: 102 LYSGLNPTDRHALDDCLELLDGSIAELKASIFDL---APSQSPALHSHDLLTLVSAAMTN 161
              GL P +  AL DCLE +D ++ EL  ++ DL      +S   H+ DL TL+S+A+TN
Sbjct: 119 -RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 162 HRTCVDGFYNSSGTVRSRVELYLGKIG-QHL-SIDLAMLKKIPGVNRA-----------T 221
             TC+DGF       + R  L  G++  +H+ S  LAM+K +   + A           T
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 222 GVDQEVLPEY--GAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVA 281
             +   L E        G+PKW+SV DRRLLQ +    K +  VA DGSG+FTTV+ AVA
Sbjct: 239 NNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAVA 298

Query: 282 AAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSA 341
           AAP  S+ RFVI+IKAG Y ENVE+ + K+N+MF+GDG GKT+I   RNVVDG TTF SA
Sbjct: 299 AAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSA 358

Query: 342 TVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFY 401
           TVA VG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQF+
Sbjct: 359 TVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFF 418

Query: 402 RECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVE 461
            +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++ 
Sbjct: 419 VKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIG 478

Query: 462 AASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYL 521
             SDL+ V  +F TYLGRPWK YSRTV ++S I  +I P GW EW+G+FALDTL Y EYL
Sbjct: 479 GTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYL 538

Query: 522 NRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTEIPF 552
           NRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL ST  PF
Sbjct: 539 NRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of CSPI07G17790.1 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.8e-137
Identity = 265/530 (50.00%), Postives = 346/530 (65.28%), Query Frame = 0

Query: 36  SSNPQVHQTARSLCNGTLFPDLCFSKLSSFPQLAS--LSPEKLAGSALNFTSREVLLAYT 95
           + N   H   +S C+ T +PDLCFS +++ P+ +    S + +   +LN T+  V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 96  NCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAP---SQSPALHSHDLLT 155
               L     + L   ++ AL DCLE +D ++ EL  ++ DL      +S + H+ DL T
Sbjct: 120 GIQKLLKR--TNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 156 LVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--IGQHLSIDLAMLKKIPGVN---RAT 215
           L+SAAMTN  TC+DGF +       R  L  G+  + +  S  LAM+K +   +     T
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 216 GVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAA 275
             +++++ E   V  G+P W+S  DRRLLQ+  +    N+VVA DGSGNF TV+ +VAAA
Sbjct: 240 SNNRKLIEETSTV-DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 299

Query: 276 PNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATV 335
           P   + R++I IKAG Y ENVE+ +   N+MF+GDG  +T+I   RNVVDG TTF+SATV
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 359

Query: 336 AVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRE 395
           AVVG GF+A+ ITF+N AGPSKHQAVALR  +D SAFY C  + YQDTLYVHS RQF+  
Sbjct: 360 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 419

Query: 396 CDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAA 455
           C + GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A 
Sbjct: 420 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGAT 479

Query: 456 SDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNR 515
           SDL PV  SF TYLGRPWK YSRTV ++S I  +I P GW EW+G FAL+TL+YGE+ N 
Sbjct: 480 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNA 539

Query: 516 GPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTEIPFFSGL 556
           G G+ T+ RV W G+RVIT+ATEA  FT   FI GSSWL ST  PF  GL
Sbjct: 540 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CSPI07G17790.1 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 4.6e-308
Identity = 530/558 (94.98%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPTSISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCFS 60
           MYGLRNRAQISSNPTSISTIS FFFIFFLFS KPISSNPQ+HQTARSLCNGTLFP+LCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFT+REVLLAY NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL PSQS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240
           LGKIGQHLSIDLAMLKKIPGVNRAT V +EVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKV-EEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300
           TKFN+VVAKDGSGNFTTV+EAVAAAPN+S+TRFVIYIKAGAYFENVEIGRAKSNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLI 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL+
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQG 540
           EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SSWLNSTEIPFFSGLTPP 559
           SSWLNST IPFFSGLT P
Sbjct: 541 SSWLNSTGIPFFSGLTSP 557

BLAST of CSPI07G17790.1 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 6.0e-308
Identity = 529/558 (94.80%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPTSISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCFS 60
           MYGLRNRAQISSNPTSISTIS FFFIFFLFS KPISSNPQ+HQTARSLCNGTLFP+LCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFT+REVLLAY NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL PSQS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240
           LGKIGQHLSIDLAMLKKIPGVNRAT V +EVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKV-EEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300
           TKFN+VVAKDGSGNFTTV+EAVAAAPN+S+TRFVIYIKAGAYFENVEIGRAKSNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVAV+G GFIAKGITFENYAGPSKHQAVALRSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLI 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL+
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQG 540
           EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SSWLNSTEIPFFSGLTPP 559
           SSWLNST IPFFSGLT P
Sbjct: 541 SSWLNSTGIPFFSGLTSP 557

BLAST of CSPI07G17790.1 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.0e-299
Identity = 512/517 (99.03%), Postives = 515/517 (99.61%), Query Frame = 0

Query: 42  HQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTH 101
           HQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTH
Sbjct: 98  HQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTH 157

Query: 102 LYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRT 161
           LYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRT
Sbjct: 158 LYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRT 217

Query: 162 CVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGG 221
           CVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQE+LPEYGAVKGG
Sbjct: 218 CVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGG 277

Query: 222 FPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGA 281
           FPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNAS+TRFVIYIKAGA
Sbjct: 278 FPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGA 337

Query: 282 YFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFEN 341
           YFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVG GFIAKGITFEN
Sbjct: 338 YFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFEN 397

Query: 342 YAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV 401
           YAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAV
Sbjct: 398 YAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAV 457

Query: 402 VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR 461
           VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR
Sbjct: 458 VFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGR 517

Query: 462 PWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYR 521
           PWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYR
Sbjct: 518 PWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYR 577

Query: 522 VITNATEASQFTVEGFIQGSSWLNSTEIPFFSGLTPP 559
           VITNATEASQFTVE FIQGSSWLNSTEIPFFSGLTPP
Sbjct: 578 VITNATEASQFTVERFIQGSSWLNSTEIPFFSGLTPP 614

BLAST of CSPI07G17790.1 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.3e-288
Identity = 498/556 (89.57%), Postives = 518/556 (93.17%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPT-SISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCF 60
           MYGLR+RAQ SSNPT SIS I   F + FLFSFKPISSNPQ HQTARSLCNGTLFP+LCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLDGSIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVN 240
           YLGKI QHLSIDLAMLKKIPGV RA    +EVLPEYGAV+GGFP WVSVKDRRLLQAAVN
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRA-ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVN 240

Query: 241 ETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVG 300
           ETKFN+VVAKDGSGNFTTV EAVAAAPN+S+TRFVIYIKAGAYFENVEI R K+NLMFVG
Sbjct: 241 ETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVG 300

Query: 301 DGIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDF 360
           DGIGKTLIKADRNVVDGWTTFRSATVA VG GFIAKGITFEN+AGPSKHQAVA RSNSDF
Sbjct: 301 DGIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDF 360

Query: 361 SAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRN 420
           SAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRN
Sbjct: 361 SAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRN 420

Query: 421 IFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQL 480
           IFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRT+FL+SFIGQL
Sbjct: 421 IFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQL 480

Query: 481 IEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQ 540
           +EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTVE FIQ
Sbjct: 481 VEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQ 540

Query: 541 GSSWLNSTEIPFFSGL 556
           GS WLNSTEIPFFSGL
Sbjct: 541 GSEWLNSTEIPFFSGL 555

BLAST of CSPI07G17790.1 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 7.1e-285
Identity = 492/556 (88.49%), Postives = 515/556 (92.63%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPT-SISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCF 60
           MYGLR+RAQ SSNPT SIS I  FF + FL SFKPISSNPQ HQTARSLCNGTLFP+LCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT+ EV  AY NC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLDGSIAEL+ SI+DL+PSQS A HSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVN 240
           YLGKI QHLSIDLAMLKKIPGV RA    +EVLPEYGAV+GGFP WVSVKDRRLLQA+VN
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRA-ATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVN 240

Query: 241 ETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVG 300
           ETKFN+VVAKDGSGNFTTV  AVAAAPN+S+TRFVIYIKAGAYFENVEI R K+NLMFVG
Sbjct: 241 ETKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVG 300

Query: 301 DGIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDF 360
           DGIGKTLIKADRNVVDGWTTFRSATV  VG GFIAKGITFEN+AGPSKHQAVA RSNSDF
Sbjct: 301 DGIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDF 360

Query: 361 SAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRN 420
           SAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAV+FQNCNLYARKPNSNQRN
Sbjct: 361 SAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRN 420

Query: 421 IFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQL 480
           IFTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKLYSRTVFL+SFIGQL
Sbjct: 421 IFTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQL 480

Query: 481 IEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQ 540
           +EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTVE FIQ
Sbjct: 481 VEPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQ 540

Query: 541 GSSWLNSTEIPFFSGL 556
           GS WLNSTEIPFFSGL
Sbjct: 541 GSEWLNSTEIPFFSGL 555

BLAST of CSPI07G17790.1 vs. NCBI nr
Match: XP_004136014.1 (pectinesterase isoform X2 [Cucumis sativus] >KGN45518.2 hypothetical protein Csa_015578 [Cucumis sativus])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 553/561 (98.57%), Postives = 556/561 (99.11%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPTS---ISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60
           MYGLRNRAQISSNPTS   ISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL
Sbjct: 1   MYGLRNRAQISSNPTSISTISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120
           CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180
           LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240
           ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQE+LPEYGAVKGGFPKWVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMF 300
           VNETKFNMVVAKDGSGNFTTVSEAVAAAPNAS+TRFVIYIKAGAYFENVEIGRAKSNLMF
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNS 360
           VGDGIGKTLIKADRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNS
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420
           DFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480
           RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480

Query: 481 QLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGF 540
           QLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVE F
Sbjct: 481 QLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVERF 540

Query: 541 IQGSSWLNSTEIPFFSGLTPP 559
           IQGSSWLNSTEIPFFSGLTPP
Sbjct: 541 IQGSSWLNSTEIPFFSGLTPP 561

BLAST of CSPI07G17790.1 vs. NCBI nr
Match: XP_031744539.1 (pectinesterase isoform X1 [Cucumis sativus])

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 553/567 (97.53%), Postives = 556/567 (98.06%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPTS---ISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60
           MYGLRNRAQISSNPTS   ISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL
Sbjct: 1   MYGLRNRAQISSNPTSISTISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120
           CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180
           LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240
           ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQE+LPEYGAVKGGFPKWVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMF 300
           VNETKFNMVVAKDGSGNFTTVSEAVAAAPNAS+TRFVIYIKAGAYFENVEIGRAKSNLMF
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKADRNVVDGWTTFRSAT------VAVVGPGFIAKGITFENYAGPSKHQAV 360
           VGDGIGKTLIKADRNVVDGWTTFRSAT      VAVVG GFIAKGITFENYAGPSKHQAV
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVGFVYYVAVVGTGFIAKGITFENYAGPSKHQAV 360

Query: 361 ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYAR 420
           ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYR+CDVYGTIDFIFGNAAVVFQNCNLYAR
Sbjct: 361 ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYAR 420

Query: 421 KPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVF 480
           KPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVF
Sbjct: 421 KPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVF 480

Query: 481 LRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQ 540
           LRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQ
Sbjct: 481 LRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQ 540

Query: 541 FTVEGFIQGSSWLNSTEIPFFSGLTPP 559
           FTVE FIQGSSWLNSTEIPFFSGLTPP
Sbjct: 541 FTVERFIQGSSWLNSTEIPFFSGLTPP 567

BLAST of CSPI07G17790.1 vs. NCBI nr
Match: XP_008451545.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1066.2 bits (2756), Expect = 9.4e-308
Identity = 530/558 (94.98%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPTSISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCFS 60
           MYGLRNRAQISSNPTSISTIS FFFIFFLFS KPISSNPQ+HQTARSLCNGTLFP+LCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFT+REVLLAY NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL PSQS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240
           LGKIGQHLSIDLAMLKKIPGVNRAT V +EVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKV-EEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300
           TKFN+VVAKDGSGNFTTV+EAVAAAPN+S+TRFVIYIKAGAYFENVEIGRAKSNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLI 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL+
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQG 540
           EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SSWLNSTEIPFFSGLTPP 559
           SSWLNST IPFFSGLT P
Sbjct: 541 SSWLNSTGIPFFSGLTSP 557

BLAST of CSPI07G17790.1 vs. NCBI nr
Match: KAA0046658.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.2e-307
Identity = 529/558 (94.80%), Postives = 541/558 (96.95%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPTSISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCFS 60
           MYGLRNRAQISSNPTSISTIS FFFIFFLFS KPISSNPQ+HQTARSLCNGTLFP+LCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFT+REVLLAY NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAELKASI DL PSQS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240
           LGKIGQHLSIDLAMLKKIPGVNRAT V +EVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKV-EEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240

Query: 241 TKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300
           TKFN+VVAKDGSGNFTTV+EAVAAAPN+S+TRFVIYIKAGAYFENVEIGRAKSNLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIK DRNVVDGWTTFRSATVAV+G GFIAKGITFENYAGPSKHQAVALRSNSDFS
Sbjct: 301 GIGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLI 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQL+
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQG 540
           EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQG 540

Query: 541 SSWLNSTEIPFFSGLTPP 559
           SSWLNST IPFFSGLT P
Sbjct: 541 SSWLNSTGIPFFSGLTSP 557

BLAST of CSPI07G17790.1 vs. NCBI nr
Match: XP_038896904.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.4e-297
Identity = 510/555 (91.89%), Postives = 527/555 (94.95%), Query Frame = 0

Query: 1   MYGLRNRAQISSNPTSISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDLCFS 60
           MYGL+NRAQI SNPTSISTIS FFFIFF FSFKPISSNPQ+HQTARSLCNGTLFP LCFS
Sbjct: 1   MYGLKNRAQIPSNPTSISTISTFFFIFFFFSFKPISSNPQIHQTARSLCNGTLFPHLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSA+NFT+REVL AY NCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLEL 120

Query: 121 LDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAEL+ASI DLAP QS A HSHDLLTL+SAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240
           LGKIGQHLSIDLAMLKKIPGVNRA     EVLPEYGAVKGGFP WVSVKDRRLLQAAVNE
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRAPAAG-EVLPEYGAVKGGFPNWVSVKDRRLLQAAVNE 240

Query: 241 TKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGD 300
           TKFN+VVAKDGSGNFTTV EAVAAAPN+S+TRFVIYIKAGAYFENVEI R K+NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGD 300

Query: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKADRNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSF+GYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLI 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQL+
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLV 480

Query: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQG 540
           +P GWLEWNGTFALDTLYYGEY+NRGPGSNTT RVTWPGYRVITNA+EASQFTVEGFIQG
Sbjct: 481 DPAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQG 540

Query: 541 SSWLNSTEIPFFSGL 556
           SSWLNST IPFFSGL
Sbjct: 541 SSWLNSTGIPFFSGL 554

BLAST of CSPI07G17790.1 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 538.9 bits (1387), Expect = 4.9e-153
Identity = 276/489 (56.44%), Postives = 353/489 (72.19%), Query Frame = 0

Query: 75  KLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFD 134
           +L  +ALN T   V L+ +N ++L   L S L+  D  A DDCLELLD ++ +L  +I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 135 LAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGT------------VRSRVELYLG 194
           L  S SP L  H++  L+SAAMTN RTC+DGF +S               V   ++  L 
Sbjct: 99  LR-SHSPEL--HNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 195 KIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETK 254
            I  H+S  LAML+ IPG +    V ++V         GFP WVS  DR LLQ  V+ETK
Sbjct: 159 NISSHVSDSLAMLENIPG-HIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETK 218

Query: 255 FNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGI 314
            N+VVA++G+GN+TT+ EA++AAPN+S TRFVIYIK G YFEN+EI R K+ +MF+GDGI
Sbjct: 219 VNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGI 278

Query: 315 GKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAF 374
           G+T+IKA+R+  DGWT F SATV V G GFIAK ++F NYAGP KHQAVALRS+SD SA+
Sbjct: 279 GRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAY 338

Query: 375 YQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFT 434
           Y+CSF  YQDT+YVHS +QFYRECD+YGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+T
Sbjct: 339 YRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYT 398

Query: 435 AQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEP 494
           AQGRE+  + TGISI++ ++ AA DLIPV ++F+ YLGRPW+LYSRTV ++SFI  L++P
Sbjct: 399 AQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDP 458

Query: 495 VGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSS 552
            GWL+W   FAL+TLYYGEY+N GPGSN T RV WPG++ I    EASQF+V  FI G+ 
Sbjct: 459 AGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNK 514

BLAST of CSPI07G17790.1 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 510.8 bits (1314), Expect = 1.4e-144
Identity = 263/482 (54.56%), Postives = 337/482 (69.92%), Query Frame = 0

Query: 81  LNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQS 140
           LN T  +V LA +N ++L+T L+  L   +R A +DCL LLD +I++L+ ++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 141 PALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSIDL 200
            +L  +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 201 AMLKKIPGVNRATGVDQEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGS 260
            ML+ I          ++  P+   V   +P W+S  D+RLL+A V ET +N+ VA DG+
Sbjct: 181 HMLQVIS--------RKKPSPKSSEVDVEYPSWLSENDQRLLEAPVQETNYNLSVAIDGT 240

Query: 261 GNFTTVSEAVAAAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRN 320
           GNFTT+++AV AAPN S TRF+IYIK G YFENVE+ + K+ +MF+GDGIGKT+IKA+R+
Sbjct: 241 GNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRS 300

Query: 321 VVDGWTTFRSATVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQD 380
            +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQD
Sbjct: 301 RIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQD 360

Query: 381 TLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQN 440
           TLYVHS +QFYRECD+YGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q 
Sbjct: 361 TLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQP 420

Query: 441 TGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTF 500
           TGISILNC++ AA DLIPV  +F+ YLGRPW+ YSRTV ++SFI  LI P GWLE    F
Sbjct: 421 TGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDF 480

Query: 501 ALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTEIPFF 555
           AL+TLYYGEY+N GPG+N   RVTWPG+R I N TEA+QFTV  FI GS+WLNST IPF 
Sbjct: 481 ALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFS 531

BLAST of CSPI07G17790.1 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 492.7 bits (1267), Expect = 4.0e-139
Identity = 269/528 (50.95%), Postives = 344/528 (65.15%), Query Frame = 0

Query: 42  HQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTH 101
           H   +S+C+ TL+P+LCFS +++       S +++  ++LN T++ V   Y     L   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 118

Query: 102 LYSGLNPTDRHALDDCLELLDGSIAELKASIFDL---APSQSPALHSHDLLTLVSAAMTN 161
              GL P +  AL DCLE +D ++ EL  ++ DL      +S   H+ DL TL+S+A+TN
Sbjct: 119 -RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 162 HRTCVDGFYNSSGTVRSRVELYLGKIG-QHL-SIDLAMLKKIPGVNRA-----------T 221
             TC+DGF       + R  L  G++  +H+ S  LAM+K +   + A           T
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 222 GVDQEVLPEY--GAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVA 281
             +   L E        G+PKW+SV DRRLLQ +    K +  VA DGSG+FTTV+ AVA
Sbjct: 239 NNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAAVA 298

Query: 282 AAPNASSTRFVIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSA 341
           AAP  S+ RFVI+IKAG Y ENVE+ + K+N+MF+GDG GKT+I   RNVVDG TTF SA
Sbjct: 299 AAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSA 358

Query: 342 TVAVVGPGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFY 401
           TVA VG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQF+
Sbjct: 359 TVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFF 418

Query: 402 RECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVE 461
            +C + GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC++ 
Sbjct: 419 VKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIG 478

Query: 462 AASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYL 521
             SDL+ V  +F TYLGRPWK YSRTV ++S I  +I P GW EW+G+FALDTL Y EYL
Sbjct: 479 GTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYL 538

Query: 522 NRGPGSNTTMRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTEIPF 552
           NRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL ST  PF
Sbjct: 539 NRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of CSPI07G17790.1 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 479.6 bits (1233), Expect = 3.5e-135
Identity = 255/526 (48.48%), Postives = 334/526 (63.50%), Query Frame = 0

Query: 43  QTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLA-YTNCTNLKTH 102
           Q     C+ +L+P+LC   L  FP   +    +L   + N T ++   A YT+     T 
Sbjct: 73  QAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTS----STI 132

Query: 103 LYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRT 162
            Y+ + P  R A D CLELLD S+  L  ++  +        HS D++T +S+AMTNH T
Sbjct: 133 TYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDT 192

Query: 163 CVDGF---YNSSGTVRSRVELYLGKIGQHLSIDLAM-------LKKIPGVNRATGVDQEV 222
           C DGF       G V+ +V   +  + + +S  LA+       L  +P VN    +  E 
Sbjct: 193 CTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEE 252

Query: 223 LPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASST 282
             E        P W+  +DR LL    +  + ++ V+KDGSG F T++EA+  AP  SS 
Sbjct: 253 TEE-------LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSR 312

Query: 283 RFVIYIKAGAY-FENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGP 342
           RFVIY+KAG Y  EN+++GR K+NLMF+GDG GKT+I   +++ D  TTF +AT A  G 
Sbjct: 313 RFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGA 372

Query: 343 GFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYG 402
           GFI + +TFENYAGP+KHQAVALR   D +  Y+C+ IGYQD LYVHS RQF+REC++YG
Sbjct: 373 GFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYG 432

Query: 403 TIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIP 462
           T+DFIFGNAAV+ Q+CN+YARKP + Q+   TAQ R+DPNQNTGISI  CK+ A  DL  
Sbjct: 433 TVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEA 492

Query: 463 VLSSFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSN 522
              S+ TYLGRPWKLYSR V++ S +G  I+P GWLEWNG FALD+LYYGEY+N+G GS 
Sbjct: 493 SKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSG 552

Query: 523 TTMRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTEIPFFSGLT 557
              RV WPGY VIT+  EAS+FTV  FI GSSWL ST + FFSGL+
Sbjct: 553 IGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CSPI07G17790.1 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 475.7 bits (1223), Expect = 5.1e-134
Identity = 252/519 (48.55%), Postives = 334/519 (64.35%), Query Frame = 0

Query: 43  QTARSLCNGTLFPDLCFSKLSSFP-QLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTH 102
           Q     C  T FP+LC   L  FP  LA+ S + L    +N T      A  +  +L   
Sbjct: 85  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLS-- 144

Query: 103 LYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRT 162
            +  + P  R A D C+ELLD S+  L  ++  +  S +      D+ T +SAA+TNH T
Sbjct: 145 -FVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSA---KPQDVTTWLSAALTNHDT 204

Query: 163 CVDGFYN-SSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEVLPEYGAV-- 222
           C +GF     G V+  +   L  + + +S  LA+       +   GV  +     G    
Sbjct: 205 CTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEER 264

Query: 223 KGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASSTRFVIYIK 282
           +  FP+W+  K+R +L+  V++ + +++V+KDG+G   T+SEA+  AP  S+ R +IY+K
Sbjct: 265 EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVK 324

Query: 283 AGAYFE-NVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGPGFIAKGI 342
           AG Y E N+++GR K NLMFVGDG GKT+I   +++ D  TTF +A+ A  G GFIA+ I
Sbjct: 325 AGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDI 384

Query: 343 TFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFG 402
           TFEN+AGP+KHQAVALR  +D +  Y+C+ IGYQDTLYVHS RQF+RECD+YGT+DFIFG
Sbjct: 385 TFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFG 444

Query: 403 NAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRT 462
           NAAVV QNC++YARKP   Q+N  TAQ R+DPNQNTGISI   +V AASDL     S +T
Sbjct: 445 NAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQT 504

Query: 463 YLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTW 522
           YLGRPWKL+SRTV++ S+IG  +   GWLEWN TFALDTLYYGEYLN GPGS    RV+W
Sbjct: 505 YLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSW 564

Query: 523 PGYRVITNATEASQFTVEGFIQGSSWLNSTEIPFFSGLT 557
           PGYRVI +  EA++FTV  FI GSSWL ST + F +GL+
Sbjct: 565 PGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O813016.9e-15256.44Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
O814152.0e-14354.56Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
P850762.2e-14276.88Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q425345.7e-13850.95Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
P839482.8e-13750.00Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BSU34.6e-30894.98Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1[more]
A0A5D3D1W66.0e-30894.80Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130... [more]
A0A0A0K5K83.0e-29999.03Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1[more]
A0A6J1JU835.3e-28889.57Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1[more]
A0A6J1GNU47.1e-28588.49Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004136014.10.0e+0098.57pectinesterase isoform X2 [Cucumis sativus] >KGN45518.2 hypothetical protein Csa... [more]
XP_031744539.10.0e+0097.53pectinesterase isoform X1 [Cucumis sativus][more]
XP_008451545.29.4e-30894.98PREDICTED: pectinesterase-like [Cucumis melo][more]
KAA0046658.11.2e-30794.80pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [... [more]
XP_038896904.14.4e-29791.89pectinesterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G02320.14.9e-15356.44Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.11.4e-14454.56Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.14.0e-13950.95pectin methylesterase 2 [more]
AT5G53370.13.5e-13548.48pectin methylesterase PCR fragment F [more]
AT3G49220.15.1e-13448.55Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..194
e-value: 2.3E-26
score: 103.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 46..177
e-value: 1.2E-21
score: 77.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 23..197
e-value: 7.8E-22
score: 75.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 238..555
e-value: 6.1E-123
score: 412.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 244..540
e-value: 6.2E-145
score: 481.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 44..200
e-value: 4.5E-26
score: 93.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 46..199
NoneNo IPR availablePANTHERPTHR31707:SF257PECTINESTERASEcoord: 42..552
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 42..552
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 47..200
e-value: 6.12469E-35
score: 126.784
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 242..554

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI07G17790CSPI07G17790gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI07G17790.1.utr3p1CSPI07G17790.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI07G17790.1.cds3CSPI07G17790.1.cds3CDS
CSPI07G17790.1.cds2CSPI07G17790.1.cds2CDS
CSPI07G17790.1.cds1CSPI07G17790.1.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI07G17790.1.utr5p1CSPI07G17790.1.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI07G17790.1CSPI07G17790.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity