CSPI06G19210.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI06G19210.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	WRKY domain-containing protein
Location	Chr6: 17384281 .. 17385448 (+)
Sequence length	710
RNA-Seq Expression	CSPI06G19210.1
Synteny	CSPI06G19210.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTGAAGCTTCCTAAGTTTGGAGGAAGGTCAGTGGGAAACTGCCTGTCAACCTAAACTCACTTCATATTTTTGCTCCTCTCTGTTCTACCATAACATTTTTTTTTTTTAATTTCAATTTCAAAACCAAGAAGTGTTTACATGTCTTACAATAGTAACCAGCACCTCACTACGTCGGAGTGTGATCTTTCCGAGCAGCCCGGGTTCGAGTTTACGGATTGGATGTTTGATGGGTGGCTGAATGAAAACTCTTCGTCTCTGACTGACTCGGTGATGTACCCAGTTTATCAAGAGGGGGAGGTTGATGAGTTTGTTGGGAACACCATTCAGCAAGGAGAGCCTAGCAGCAGTAAGCACATAACTCTTATTACATTTTAGCATTTGTGAGTTTTTATATCTTTGGGTTCTGTATCAGTCAGGTGGAATCTTCTTGCTAGCCACCATTAAAGATTGTACTGCTATTTATATTTCTAGAAGCAGTGGTACTAATATCTCTTTTACCTCTCCCCTTTTTGCATTTCTCTCTCCTACTTGTCAAAAACTTGATGAGATCGCCAATGAGTAGCTAAATATATTATCTCTTTTGAGGTTGTGATCTCACCTCGTGTATGATCTTGAATGGTGTTTGTAGGAGACTATGGGAGAGAGAGGGAAATTAGAGAAAGATTTGCATTCAAGACAAAATCAGAAGTTGAGATTTTGGATGATGGTTTCAAGTGGAGGAAATATGGGAAGAAGATGGTGAAGAACAGCCCAAATCCAAGGTAAGAAAACAAAAGGACTAGTAATGTTCTTAATTTAGCATTAAATTTTGTCCTAAGATAAAATGTTACAATCCTAGAAACATTAAACCAAAGATGATCAAGAAACCCAATGAAAACAGGTCTGTTTGTGTTAATTGTTAATCTGAATTGGCTCAACCAACATCTGTTAAATGCAGGAACTACTACAAATGCTTAGTCGAAGGCTGCCCAGTGAAGAAGAGAGTCGAAAGAGATCGAGAGGATCCAAAATACGTGATAACGACGTATGAGGGTGTTCATACTCATGAAAGCTCTTGATTTGTTTATTTTTACATAAAAACTTTCAATGTTTAATAAGAAATTTTGGATTTAGTGTGGGGAAATTGAAACAGTAAGAAAATTAATAGGTTGATAATACCAAACAGC

mRNA sequence

CTTTGAAGCTTCCTAAGTTTGGAGGAAGGTCAGTGGGAAACTGCCTGTCAACCTAAACTCACTTCATATTTTTGCTCCTCTCTGTTCTACCATAACATTTTTTTTTTTTAATTTCAATTTCAAAACCAAGAAGTGTTTACATGTCTTACAATAGTAACCAGCACCTCACTACGTCGGAGTGTGATCTTTCCGAGCAGCCCGGGTTCGAGTTTACGGATTGGATGTTTGATGGGTGGCTGAATGAAAACTCTTCGTCTCTGACTGACTCGGTGATGTACCCAGTTTATCAAGAGGGGGAGGTTGATGAGTTTGTTGGGAACACCATTCAGCAAGGAGAGCCTAGCAGCAGAGACTATGGGAGAGAGAGGGAAATTAGAGAAAGATTTGCATTCAAGACAAAATCAGAAGTTGAGATTTTGGATGATGGTTTCAAGTGGAGGAAATATGGGAAGAAGATGGTGAAGAACAGCCCAAATCCAAGGAACTACTACAAATGCTTAGTCGAAGGCTGCCCAGTGAAGAAGAGAGTCGAAAGAGATCGAGAGGATCCAAAATACGTGATAACGACGTATGAGGGTGTTCATACTCATGAAAGCTCTTGATTTGTTTATTTTTACATAAAAACTTTCAATGTTTAATAAGAAATTTTGGATTTAGTGTGGGGAAATTGAAACAGTAAGAAAATTAATAGGTTGATAATACCAAACAGC

Coding sequence (CDS)

ATGTCTTACAATAGTAACCAGCACCTCACTACGTCGGAGTGTGATCTTTCCGAGCAGCCCGGGTTCGAGTTTACGGATTGGATGTTTGATGGGTGGCTGAATGAAAACTCTTCGTCTCTGACTGACTCGGTGATGTACCCAGTTTATCAAGAGGGGGAGGTTGATGAGTTTGTTGGGAACACCATTCAGCAAGGAGAGCCTAGCAGCAGAGACTATGGGAGAGAGAGGGAAATTAGAGAAAGATTTGCATTCAAGACAAAATCAGAAGTTGAGATTTTGGATGATGGTTTCAAGTGGAGGAAATATGGGAAGAAGATGGTGAAGAACAGCCCAAATCCAAGGAACTACTACAAATGCTTAGTCGAAGGCTGCCCAGTGAAGAAGAGAGTCGAAAGAGATCGAGAGGATCCAAAATACGTGATAACGACGTATGAGGGTGTTCATACTCATGAAAGCTCTTGA

Protein sequence

MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGNTIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDREDPKYVITTYEGVHTHESS*

Homology

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy Swiss-Prot
Match: Q8VWQ5 (Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.4e-32
Identity = 61/78 (78.21%), Postives = 71/78 (91.03%), Query Frame = 0


Query: 75  EREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDR 134
           +++I+ R AFKT+SEVE+LDDGFKWRKYGKKMVKNSP+PRNYYKC V+GCPVKKRVERDR
Sbjct: 94  KKKIKGRVAFKTRSEVEVLDDGFKWRKYGKKMVKNSPHPRNYYKCSVDGCPVKKRVERDR 153

Query: 135 EDPKYVITTYEGVHTHES 153
           +DP +VITTYEG H H S
Sbjct: 154 DDPSFVITTYEGSHNHSS 171

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy Swiss-Prot
Match: Q93WU9 (Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.9e-27
Identity = 65/141 (46.10%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 15  DLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGNTIQQGEPS---SRD 74
           D S    F+  +   +G + E  SS T  V    +           T+ + E +   S++
Sbjct: 28  DFSNLMFFDIDEGGNNGLIEEEISSPTSIVSSETFTGESGGSGSATTLSKKESTNRGSKE 87

Query: 75  YGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVE 134
             + +E   R AF+T+S+++++DDGFKWRKYGKK VKN+ N RNYYKC  EGC VKKRVE
Sbjct: 88  SDQTKETGHRVAFRTRSKIDVMDDGFKWRKYGKKSVKNNINKRNYYKCSSEGCSVKKRVE 147

Query: 135 RDREDPKYVITTYEGVHTHES 153
           RD +D  YVITTYEGVH HES
Sbjct: 148 RDGDDAAYVITTYEGVHNHES 168

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 7.5e-24
Identity = 57/117 (48.72%), Postives = 76/117 (64.96%), Query Frame = 0


Query: 34  NENSSSLTDSVMYPVYQEGEVDEFVGNTIQQGEPSSRDYGREREIRERFAFKTKSEVEIL 93
           N+N+S     V     +EG+  +    T  Q +   ++  + RE   RFAF TKS+++ L
Sbjct: 165 NDNNSG--KEVTVKDQEEGDQQQEQKGTKPQLKAKKKNQKKARE--ARFAFLTKSDIDNL 224

Query: 94  DDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDREDPKYVITTYEGVHTH 151
           DDG++WRKYG+K VKNSP PR+YY+C   GC VKKRVER  +DP  V+TTYEG HTH
Sbjct: 225 DDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTTYEGQHTH 277

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy Swiss-Prot
Match: Q9S763 (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.2e-23
Identity = 51/90 (56.67%), Postives = 69/90 (76.67%), Query Frame = 0


Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           T  + +P S+   +ERE   R+AF+T+S+V+ILDDG++WRKYG+K VKN+P PR+YYKC 
Sbjct: 34  TSSEEKPRSKKKKKERE--ARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCT 93

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTH 151
            EGC VKK+V+R   D   V+TTY+GVHTH
Sbjct: 94  EEGCRVKKQVQRQWGDEGVVVTTYQGVHTH 121

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-23
Identity = 56/122 (45.90%), Postives = 78/122 (63.93%), Query Frame = 0


Query: 30  DGWLNENSSSLTDSVMYPVYQEGEVD-EFVGNTIQQGEPSSRDYGREREIRERFAFKTKS 89
           +G   EN++  +D +     ++ E D   VG + +Q     +  G ++E   R AF TKS
Sbjct: 76  EGEPKENTNDKSDQM-----EDNEGDLHGVGESSKQLTKQGKKKGEKKEREVRVAFMTKS 135

Query: 90  EVEILDDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDREDPKYVITTYEGVH 149
           E++ L+DG++WRKYG+K VKNSP PR+YY+C  + C VKKRVER  +DP  VITTYEG H
Sbjct: 136 EIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYEGKH 192

Query: 150 TH 151
            H
Sbjct: 196 NH 192

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy TrEMBL
Match: A0A0A0KD40 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G363560 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.9e-83
Identity = 151/153 (98.69%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLTTSE DLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKC 
Sbjct: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCS 120

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy TrEMBL
Match: A0A5A7VG90 (Putative WRKY transcription factor 50 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00250 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.1e-81
Identity = 147/153 (96.08%), Postives = 150/153 (98.04%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLTTSE DLSEQPGFEFTDWMFDGWLNENSS+LTDSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSTLTDSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           TIQQ EPSSRD+GRERE+RERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKC 
Sbjct: 61  TIQQAEPSSRDHGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCS 120

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy TrEMBL
Match: A0A1S3BCG1 (probable WRKY transcription factor 50 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488510 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.1e-81
Identity = 147/153 (96.08%), Postives = 150/153 (98.04%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLTTSE DLSEQPGFEFTDWMFDGWLNENSS+LTDSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSTLTDSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           TIQQ EPSSRD+GRERE+RERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKC 
Sbjct: 61  TIQQAEPSSRDHGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCS 120

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy TrEMBL
Match: A0A5D3BLP6 (Putative WRKY transcription factor 50 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00310 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.7e-77
Identity = 146/174 (83.91%), Postives = 149/174 (85.63%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLTTSE DLSEQPGFEFTDWMFDGWLNENSS+LTDSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSTLTDSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSR---------------------DYGREREIRERFAFKTKSEVEILDDGFKW 120
           TIQQ EPSS                      D+GRERE+RERFAFKTKSEVEILDDGFKW
Sbjct: 61  TIQQAEPSSSGVFSPATIKDCTAFYISRSSGDHGREREVRERFAFKTKSEVEILDDGFKW 120

Query: 121 RKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           RKYGKKMVKNSPNPRNYYKC VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 RKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 174

[Close]

BLAST of CSPI06G19210.1 vs. ExPASy TrEMBL
Match: A0A6J1BQD9 (probable WRKY transcription factor 50 OS=Momordica charantia OX=3673 GN=LOC111004843 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 8.2e-74
Identity = 137/155 (88.39%), Postives = 144/155 (92.90%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEG-EVDEFVG 60
           MSYNSNQHL TSE DL+EQPGFEF DWMFDGWLNENSSSL +SVMYPVY+ G EVDE VG
Sbjct: 1   MSYNSNQHLATSESDLAEQPGFEFPDWMFDGWLNENSSSLAESVMYPVYKAGEEVDEIVG 60

Query: 61  -NTIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYK 120
            NTI QGEPSSRD GRERE+RERFAFKTKSE+EILDDGFKWRKYGKKMVKNSPNPRNYYK
Sbjct: 61  INTIHQGEPSSRDRGREREVRERFAFKTKSEIEILDDGFKWRKYGKKMVKNSPNPRNYYK 120

Query: 121 CLVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           C +EGCPVKKRVERDREDP+YVITTYEGVHTHESS
Sbjct: 121 CSIEGCPVKKRVERDREDPRYVITTYEGVHTHESS 155

[Close]

BLAST of CSPI06G19210.1 vs. NCBI nr
Match: XP_004144271.1 (probable WRKY transcription factor 50 [Cucumis sativus])

HSP 1 Score: 318.2 bits (814), Expect = 4.0e-83
Identity = 151/153 (98.69%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLTTSE DLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKC 
Sbjct: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCS 120

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of CSPI06G19210.1 vs. NCBI nr
Match: XP_008445519.1 (PREDICTED: probable WRKY transcription factor 50 isoform X1 [Cucumis melo] >KAA0064721.1 putative WRKY transcription factor 50 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 312.4 bits (799), Expect = 2.2e-81
Identity = 147/153 (96.08%), Postives = 150/153 (98.04%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLTTSE DLSEQPGFEFTDWMFDGWLNENSS+LTDSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSTLTDSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           TIQQ EPSSRD+GRERE+RERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKC 
Sbjct: 61  TIQQAEPSSRDHGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCS 120

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of CSPI06G19210.1 vs. NCBI nr
Match: TYK00741.1 (putative WRKY transcription factor 50 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 297.7 bits (761), Expect = 5.6e-77
Identity = 146/174 (83.91%), Postives = 149/174 (85.63%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLTTSE DLSEQPGFEFTDWMFDGWLNENSS+LTDSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSTLTDSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSR---------------------DYGREREIRERFAFKTKSEVEILDDGFKW 120
           TIQQ EPSS                      D+GRERE+RERFAFKTKSEVEILDDGFKW
Sbjct: 61  TIQQAEPSSSGVFSPATIKDCTAFYISRSSGDHGREREVRERFAFKTKSEVEILDDGFKW 120

Query: 121 RKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           RKYGKKMVKNSPNPRNYYKC VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 RKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 174

[Close]

BLAST of CSPI06G19210.1 vs. NCBI nr
Match: XP_038884623.1 (probable WRKY transcription factor 50 isoform X2 [Benincasa hispida])

HSP 1 Score: 293.9 bits (751), Expect = 8.1e-76
Identity = 139/153 (90.85%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHLT SE DL E+PGFEFT+WMFDGWLNE SSSL DSVMYPVYQEGEVDEFVGN
Sbjct: 1   MSYNSNQHLTMSESDLFEKPGFEFTNWMFDGWLNETSSSLADSVMYPVYQEGEVDEFVGN 60

Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           TI QGEPS+RD GRERE+ ERFAFKTKSEVEILDDGFKWRKYGKK VKNSPNPRNYYKC 
Sbjct: 61  TIHQGEPSNRDSGREREVGERFAFKTKSEVEILDDGFKWRKYGKKKVKNSPNPRNYYKCS 120

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of CSPI06G19210.1 vs. NCBI nr
Match: XP_023546261.1 (probable WRKY transcription factor 50 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288.9 bits (738), Expect = 2.6e-74
Identity = 135/153 (88.24%), Postives = 141/153 (92.16%), Query Frame = 0


Query: 1   MSYNSNQHLTTSECDLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGN 60
           MSYNSNQHL  SE DL EQPGFEFTDWMFD WL+ENS  L DS MYPVYQ GE++EFVG+
Sbjct: 1   MSYNSNQHLVMSENDLPEQPGFEFTDWMFDRWLDENSPPLVDSAMYPVYQSGEIEEFVGS 60

Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           TIQQGEPSSRD GRERE+RERFAFKTKSEVE+LDDGFKWRKYGKKMVKNSPNPRNYYKC 
Sbjct: 61  TIQQGEPSSRDSGREREVRERFAFKTKSEVEVLDDGFKWRKYGKKMVKNSPNPRNYYKCS 120

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 154
           VEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of CSPI06G19210.1 vs. TAIR 10
Match: AT5G26170.1 (WRKY DNA-binding protein 50 )

HSP 1 Score: 139.0 bits (349), Expect = 3.1e-33
Identity = 61/78 (78.21%), Postives = 71/78 (91.03%), Query Frame = 0


Query: 75  EREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDR 134
           +++I+ R AFKT+SEVE+LDDGFKWRKYGKKMVKNSP+PRNYYKC V+GCPVKKRVERDR
Sbjct: 94  KKKIKGRVAFKTRSEVEVLDDGFKWRKYGKKMVKNSPHPRNYYKCSVDGCPVKKRVERDR 153

Query: 135 EDPKYVITTYEGVHTHES 153
           +DP +VITTYEG H H S
Sbjct: 154 DDPSFVITTYEGSHNHSS 171

[Close]

BLAST of CSPI06G19210.1 vs. TAIR 10
Match: AT5G64810.1 (WRKY DNA-binding protein 51 )

HSP 1 Score: 123.6 bits (309), Expect = 1.4e-28
Identity = 65/141 (46.10%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 15  DLSEQPGFEFTDWMFDGWLNENSSSLTDSVMYPVYQEGEVDEFVGNTIQQGEPS---SRD 74
           D S    F+  +   +G + E  SS T  V    +           T+ + E +   S++
Sbjct: 28  DFSNLMFFDIDEGGNNGLIEEEISSPTSIVSSETFTGESGGSGSATTLSKKESTNRGSKE 87

Query: 75  YGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVE 134
             + +E   R AF+T+S+++++DDGFKWRKYGKK VKN+ N RNYYKC  EGC VKKRVE
Sbjct: 88  SDQTKETGHRVAFRTRSKIDVMDDGFKWRKYGKKSVKNNINKRNYYKCSSEGCSVKKRVE 147

Query: 135 RDREDPKYVITTYEGVHTHES 153
           RD +D  YVITTYEGVH HES
Sbjct: 148 RDGDDAAYVITTYEGVHNHES 168

[Close]

BLAST of CSPI06G19210.1 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 111.7 bits (278), Expect = 5.3e-25
Identity = 57/117 (48.72%), Postives = 76/117 (64.96%), Query Frame = 0


Query: 34  NENSSSLTDSVMYPVYQEGEVDEFVGNTIQQGEPSSRDYGREREIRERFAFKTKSEVEIL 93
           N+N+S     V     +EG+  +    T  Q +   ++  + RE   RFAF TKS+++ L
Sbjct: 165 NDNNSG--KEVTVKDQEEGDQQQEQKGTKPQLKAKKKNQKKARE--ARFAFLTKSDIDNL 224

Query: 94  DDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDREDPKYVITTYEGVHTH 151
           DDG++WRKYG+K VKNSP PR+YY+C   GC VKKRVER  +DP  V+TTYEG HTH
Sbjct: 225 DDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTTYEGQHTH 277

[Close]

BLAST of CSPI06G19210.1 vs. TAIR 10
Match: AT3G01970.1 (WRKY DNA-binding protein 45 )

HSP 1 Score: 110.2 bits (274), Expect = 1.5e-24
Identity = 51/90 (56.67%), Postives = 69/90 (76.67%), Query Frame = 0


Query: 61  TIQQGEPSSRDYGREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCL 120
           T  + +P S+   +ERE   R+AF+T+S+V+ILDDG++WRKYG+K VKN+P PR+YYKC 
Sbjct: 34  TSSEEKPRSKKKKKERE--ARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCT 93

Query: 121 VEGCPVKKRVERDREDPKYVITTYEGVHTH 151
            EGC VKK+V+R   D   V+TTY+GVHTH
Sbjct: 94  EEGCRVKKQVQRQWGDEGVVVTTYQGVHTH 121

[Close]

BLAST of CSPI06G19210.1 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-24
Identity = 56/122 (45.90%), Postives = 78/122 (63.93%), Query Frame = 0


Query: 30  DGWLNENSSSLTDSVMYPVYQEGEVD-EFVGNTIQQGEPSSRDYGREREIRERFAFKTKS 89
           +G   EN++  +D +     ++ E D   VG + +Q     +  G ++E   R AF TKS
Sbjct: 76  EGEPKENTNDKSDQM-----EDNEGDLHGVGESSKQLTKQGKKKGEKKEREVRVAFMTKS 135

Query: 90  EVEILDDGFKWRKYGKKMVKNSPNPRNYYKCLVEGCPVKKRVERDREDPKYVITTYEGVH 149
           E++ L+DG++WRKYG+K VKNSP PR+YY+C  + C VKKRVER  +DP  VITTYEG H
Sbjct: 136 EIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYEGKH 192

Query: 150 TH 151
            H
Sbjct: 196 NH 192

The following BLAST results are available for this feature:

BLAST of CSPI06G19210.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VWQ5	4.4e-32	78.21	Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 ...	[more]
Q93WU9	1.9e-27	46.10	Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 ...	[more]
Q9FGZ4	7.5e-24	48.72	Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ...	[more]
Q9S763	2.2e-23	56.67	Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 ...	[more]
Q93WV4	2.8e-23	45.90	WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1	[more]

BLAST of CSPI06G19210.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KD40	1.9e-83	98.69	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G363560 PE=4 S...	[more]
A0A5A7VG90	1.1e-81	96.08	Putative WRKY transcription factor 50 isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BCG1	1.1e-81	96.08	probable WRKY transcription factor 50 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5D3BLP6	2.7e-77	83.91	Putative WRKY transcription factor 50 isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A6J1BQD9	8.2e-74	88.39	probable WRKY transcription factor 50 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]

BLAST of CSPI06G19210.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144271.1	4.0e-83	98.69	probable WRKY transcription factor 50 [Cucumis sativus]	[more]
XP_008445519.1	2.2e-81	96.08	PREDICTED: probable WRKY transcription factor 50 isoform X1 [Cucumis melo] >KAA0...	[more]
TYK00741.1	5.6e-77	83.91	putative WRKY transcription factor 50 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_038884623.1	8.1e-76	90.85	probable WRKY transcription factor 50 isoform X2 [Benincasa hispida]	[more]
XP_023546261.1	2.6e-74	88.24	probable WRKY transcription factor 50 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI06G19210.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26170.1	3.1e-33	78.21	WRKY DNA-binding protein 50	[more]
AT5G64810.1	1.4e-28	46.10	WRKY DNA-binding protein 51	[more]
AT5G49520.1	5.3e-25	48.72	WRKY DNA-binding protein 48	[more]
AT3G01970.1	1.5e-24	56.67	WRKY DNA-binding protein 45	[more]
AT1G29860.1	2.0e-24	45.90	WRKY DNA-binding protein 71	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 93..152 e-value: 2.4E-35 score: 133.4
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 94..151 e-value: 8.7E-25 score: 86.7
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 88..153 score: 30.159225
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 80..146 e-value: 3.7E-32 score: 111.8
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 86..151
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 28..152
None	No IPR available	PANTHER	PTHR31221:SF112	WRKY TRANSCRIPTION FACTOR 59-RELATED	coord: 28..152

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI06G19210	CSPI06G19210	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G19210.1.utr5p1	CSPI06G19210.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G19210.1.cds1	CSPI06G19210.1.cds1	CDS
CSPI06G19210.1.cds2	CSPI06G19210.1.cds2	CDS
CSPI06G19210.1.cds3	CSPI06G19210.1.cds3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G19210.1.utr3p1	CSPI06G19210.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI06G19210.1	CSPI06G19210.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity