CSPI05G31250.1 (mRNA) Cucumber (PI 183967) v1

BLAST of CSPI05G31250.1 vs. ExPASy Swiss-Prot
Match: Q9LFE0 (SART-1 family protein DOT2 OS=Arabidopsis thaliana OX=3702 GN=DOT2 PE=1 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 3.4e-225
Identity = 491/877 (55.99%), Postives = 625/877 (71.27%), Query Frame = 0

Query: 311  DSERDRVRSREKR--------KEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERERDRK 370
            +  + R   RE+R        +E RD   KE+   SK K+KDYDR+  +DK++ R+   K
Sbjct: 4    EKSKSRHEIREERADYEGSPVREHRDGRRKEKDHRSKDKEKDYDREKIRDKDHRRD---K 63

Query: 371  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG 430
            ++ +DR+R R+ + EK+  RG DKER K+K RDR K++D++ K++++ KDR NE + EK 
Sbjct: 64   EKERDRKRSRDEDTEKEISRGRDKEREKDKSRDRVKEKDKE-KERNRHKDRENERDNEKE 123

Query: 431  REKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFGDE 490
            ++K R +          K+R  ++  EDD +T +  ++                      
Sbjct: 124  KDKDRARV---------KERASKKSHEDDDETHKAAERY--------------------- 183

Query: 491  GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK 550
                      E++ +R +N+G         +N D    G  AS+  L+ RI  M+E+R K
Sbjct: 184  ----------EHSDNRGLNEGG--------DNVDAASSGKEASALDLQNRILKMREERKK 243

Query: 551  KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH 610
            K  + S+ LSWV RSRK+EEK+ +EK++A QLS+IFEEQDN++QG +      ED  +  
Sbjct: 244  KAEDASDALSWVARSRKIEEKRNAEKQRAQQLSRIFEEQDNLNQGEN------EDGEDGE 303

Query: 611  DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA 670
             L+GVKVLHG++KV+EGGAV+LTLKDQS+L DG+VN E+D+LENVEIGEQK+R+ AY+AA
Sbjct: 304  HLSGVKVLHGLEKVVEGGAVILTLKDQSVLTDGDVNNEIDMLENVEIGEQKRRNEAYEAA 363

Query: 671  KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG 730
            KKK GIYDDKFND+   EKKMLPQYD+ A  DEG+ LD +G F  +AEKKLEELR+R+QG
Sbjct: 364  KKKKGIYDDKFNDDPGAEKKMLPQYDEAA-TDEGIFLDAKGRFTGEAEKKLEELRKRIQG 423

Query: 731  ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV 790
              +   FEDLN S KVS DY++Q+EMLKFKKP+KKK LRKK+KLD+  LEAEA+++GLG 
Sbjct: 424  -QTTHTFEDLNSSAKVSSDYFSQEEMLKFKKPKKKKQLRKKDKLDLSMLEAEAVASGLGA 483

Query: 791  GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA 850
             DLGSR D RRQA KEE+E+ E E R NAYQ A AKADEASR L+  Q    + ++++  
Sbjct: 484  EDLGSRKDGRRQAMKEEKERIEYEKRSNAYQEAIAKADEASRLLRREQVQPFKRDEDESM 543

Query: 851  LIADDDEDFYKSLERARKLAL-KKQDAASGPGAVALLATATTSSQATDDQSTKAGELQEN 910
            ++ADD ED YKSLE+AR+LAL KK++A SGP AVA L  A++++Q TDD +T   E QEN
Sbjct: 544  VLADDAEDLYKSLEKARRLALIKKEEAGSGPQAVAHL-VASSTNQTTDDNTTTGDETQEN 603

Query: 911  KVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAME 970
             VVFTEM +FVWGLQ + D  KPE +DVFM++D  PK    E  ++   G TEV DT M+
Sbjct: 604  TVVFTEMGDFVWGLQRENDVRKPESEDVFMEEDVAPKAPV-EVKEEHPDGLTEVNDTDMD 663

Query: 971  ES-TPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI 1030
             +    + + + PDE IHEV VGKGLS ALKLLKDRGTLKE +EWGGRNMDK+KSKLVGI
Sbjct: 664  AAEDSSDTKEITPDENIHEVAVGKGLSGALKLLKDRGTLKEKVEWGGRNMDKKKSKLVGI 723

Query: 1031 VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK 1090
            VD+D  KESK K+S+     D  K+I IERTDEFGR +TPKE+FR LSHKFHGKGPGKMK
Sbjct: 724  VDDDGGKESKDKESK-----DRFKDIRIERTDEFGRTLTPKEAFRLLSHKFHGKGPGKMK 783

Query: 1091 QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF 1150
            +EKRMKQYQEELKLKQMKN+DTPS SV+RMREAQAQLKTPYLVLSGHVKPGQTSDP+SGF
Sbjct: 784  EEKRMKQYQEELKLKQMKNSDTPSQSVQRMREAQAQLKTPYLVLSGHVKPGQTSDPQSGF 813

Query: 1151 ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGT 1178
            ATVEKD+PG LTPMLGDRKVEHFLGIKRK E  N+ T
Sbjct: 844  ATVEKDVPGSLTPMLGDRKVEHFLGIKRKSEPGNSDT 813

BLAST of CSPI05G31250.1 vs. ExPASy Swiss-Prot
Match: O43290 (U4/U6.U5 tri-snRNP-associated protein 1 OS=Homo sapiens OX=9606 GN=SART1 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 8.3e-22
Identity = 210/808 (25.99%), Postives = 377/808 (46.66%), Query Frame = 0

Query: 370  RERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKGREKHRDQ 429
            R RE +  K    G     G+ + R R++  +R   ++  + +  +      GRE+ + +
Sbjct: 31   RHREHKKHKHRSGGSGGSGGERRKRSRERGGERGSGRRGAEAEARSSTH---GRERSQAE 90

Query: 430  EDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFGDEGKDYMLE 489
              +   +   +D G E           +K    D   +  +   +   + G +  +    
Sbjct: 91   PSERRVKREKRDDGYE-------AAASSKTSSGDASSLSIEETNKLRAKLGLKPLEVNAI 150

Query: 490  SDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLKKQTEE-- 549
                  ++  V   +++  + + +  + L+    A+    E+R+ N K  ++K   E+  
Sbjct: 151  KKEAGTKEEPVT-ADVINPMALRQR-EELREKLAAAK---EKRLLNQKLGKIKTLGEDDP 210

Query: 550  --SEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVS---DDDIAP--EDTTN 609
               +  +W++RSR+L++    EK+ A + +K+ EE D  + GVS   +++     +D  +
Sbjct: 211  WLDDTAAWIERSRQLQK----EKDLAEKRAKLLEEMDQ-EFGVSTLVEEEFGQRRQDLYS 270

Query: 610  NHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYK 669
              DL G+ V H +D   EG  ++LTLKD+ +L      EE DVL NV + ++++ +   +
Sbjct: 271  ARDLQGLTVEHAIDSFREGETMILTLKDKGVL-----QEEEDVLVNVNLVDKERAEKNVE 330

Query: 670  AAKKKTGIYDDKFNDENYGE------KKMLPQYDDPADAD--EGLTLDGRGGFNNDAEKK 729
              KKK   Y     DE+  +      + +L +YD+  + +      L+  G  +   E++
Sbjct: 331  LRKKKPD-YLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERE 390

Query: 730  LEELRRRLQ-GASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKK-KSLRKKEK-LDID 789
            LEE+R +L+  A S+       V  +++ +Y T +EM+ FKK +++ K +RKKEK + + 
Sbjct: 391  LEEIRAKLRLQAQSLS-----TVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVR 450

Query: 790  ALEAEAISAGLGVGDLGS--RNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQ 849
            A +   +      GD GS  R   RR+  + E+EK      L +  +     D +     
Sbjct: 451  ADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDE--- 510

Query: 850  LVQNSSARLEDNDDALIADDDE-DFYKSLE---RARKLALKKQDAASGPGAVALLATATT 909
              +   A    +   L  D+ E +  K LE   R R+L   +Q   SG   V ++    +
Sbjct: 511  --EEGGAPPPGSPQVLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLES 570

Query: 910  SSQATDDQSTKAGELQENKVVFTEMEEF--------VWGLQLDEDAHKPEEDDVFMD--- 969
              +  ++        ++  +VF    EF         +GL     A   EE +  MD   
Sbjct: 571  RQRGWEEDEDPE---RKGAIVFNATSEFCRTLGEIPTYGL-----AGNREEQEELMDFER 630

Query: 970  DDEIPKEEYHEDVKDKDGGWTEVKDTAMEESTPEENEAVAPDETIHEVP-VGKGLSSALK 1029
            D+E       E   +++ GW+ V    ++E   +++ + +    + E P V +GL++AL 
Sbjct: 631  DEERSANGGSESDGEENIGWSTVN---LDEEKQQQDFSASSTTILDEEPIVNRGLAAALL 690

Query: 1030 LLKDRGTLKESIEWGGRNMDKRKS--KLVGIVDED---EPKESKSKDSR-----LSSLVD 1089
            L +++G L+ +++   R     KS    V  +++    + K S+ ++ R           
Sbjct: 691  LCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDG 750

Query: 1090 YKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNAD 1130
            YK ++ IE  DE GR +TPKE+FRQLSH+FHGKG GKMK E+RMK+  EE  LK+M ++D
Sbjct: 751  YKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSD 791

BLAST of CSPI05G31250.1 vs. ExPASy Swiss-Prot
Match: Q9Z315 (U4/U6.U5 tri-snRNP-associated protein 1 OS=Mus musculus OX=10090 GN=Sart1 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 8.3e-22
Identity = 212/807 (26.27%), Postives = 378/807 (46.84%), Query Frame = 0

Query: 370  RERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKGREKHRDQ 429
            R RE +  K    G     G+ + R R++  +R   ++  + +  +      GRE+ + +
Sbjct: 31   RHREHKKHKHRSSGGGSSGGERRKRSRERGSERGSGRRGAEAEARSGAH---GRERSQAE 90

Query: 430  EDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFGDEGKDYMLE 489
              +   +   +D G E           +K    D   +  +   +   + G +  +    
Sbjct: 91   PSERRVKREKRDDGYE-------AAASSKASSGDASSLSIEETNKLRAKLGLKPLEVNAV 150

Query: 490  SDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLKKQTEE-- 549
                  ++  V   +++  + + +  + L+    A+    E+R+ N K  ++K   E+  
Sbjct: 151  KKEAGTKEEPV-AADVINPMALRQR-EELREKLAAAK---EKRLLNQKLGKIKTLGEDDP 210

Query: 550  --SEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVS-----DDDIAPEDTTN 609
               +  +W++RSR+L++    EK+ A + +K+ EE D  + GVS     + +   +D  +
Sbjct: 211  WLDDTAAWIERSRQLQK----EKDLAEKRAKLLEEMDQ-EFGVSTLVEEEFEQRRQDLYS 270

Query: 610  NHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYK 669
              DL G+ V H +D   EG  VVLTLKD+ +L DG      DVL NV + ++++ D   +
Sbjct: 271  ARDLQGLTVEHAIDSFREGETVVLTLKDKGVLQDGE-----DVLVNVNMVDKERADKNVE 330

Query: 670  AAKKKTGIYDDKFNDENYGE------KKMLPQYDDPADAD--EGLTLDGRGGFNNDAEKK 729
              KKK   Y     DE+  +      + +L +YD+  + +      L+  G  +   E++
Sbjct: 331  LRKKKPD-YLPYAEDESVDDLAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERE 390

Query: 730  LEELRRRLQ-GASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKK-KSLRKKEK-LDID 789
            LEE+R +L+  A S+      +V  +++ +Y + +EM+ FKK +++ K +RKKEK + + 
Sbjct: 391  LEEIRTKLRLQAQSLS-----SVGPRLASEYLSPEEMVTFKKTKRRVKKIRKKEKEVIMR 450

Query: 790  ALEAEAISAGLGVGDLGS--RNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEA-SRSL 849
            A +   +      GD GS  R   RR+  + E+E  E E +    Q   +      +  +
Sbjct: 451  ADDLLPLGDQTQDGDFGSRLRGRGRRRVPEVEEEALEDEEKDPVAQPPPSDDTRVENMDI 510

Query: 850  QLVQNSSARLEDNDDALIADDDE-DFYKSLERARKL-ALKKQDAASGPGAVALLATATTS 909
               ++  A    + + L  D+ E +  K LE+ R+L  L++       G   L       
Sbjct: 511  SDEEDGGALPPGSPEGLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVLEIVKKLE 570

Query: 910  SQATDDQSTKAGELQENKVVFTEMEEF--------VWGLQLDEDAHKPEEDDVFMD---D 969
            S+    +  +  E ++  +VF    EF         +GL     A   EE +  MD   D
Sbjct: 571  SRQRGWEEEEDPE-RKGTIVFNATSEFCRTLGEIPTYGL-----AGNREEQEELMDFERD 630

Query: 970  DEIPKEEYHEDVKDKDGGWTEVKDTAMEESTPEENEAVAPDETIHEVP-VGKGLSSALKL 1029
            +E       E   +++ GW+ V    ++E    ++ + +    + E P V +GL++AL L
Sbjct: 631  EERSANGGSESDGEENIGWSTVN---LDEEKQHQDFSASSTTILDEEPIVNRGLAAALLL 690

Query: 1030 LKDRGTLKESIEWGGRNMDKRKS--KLVGIVDED---EPKESKSKDSR-----LSSLVDY 1089
             +++G L+ +++   R     KS    V  +++    + K S+ ++ R           Y
Sbjct: 691  CQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGY 750

Query: 1090 KKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADT 1130
            K ++ IE  DE GR +TPKE+FRQLSH+FHGKG GKMK E+RMK+  EE  LK+M ++DT
Sbjct: 751  KPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDT 797

BLAST of CSPI05G31250.1 vs. ExPASy Swiss-Prot
Match: Q5XIW8 (U4/U6.U5 tri-snRNP-associated protein 1 OS=Rattus norvegicus OX=10116 GN=Sart1 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 3.2e-21
Identity = 211/808 (26.11%), Postives = 372/808 (46.04%), Query Frame = 0

Query: 370  RERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKGREKHRDQ 429
            R RE +  K    G     G+ + R R++  +R   ++  + +  +      GRE+ + +
Sbjct: 31   RHREHKKHKHRSSGGGSSGGERRKRSRERGAERGSGRRGAEAEARSGAH---GRERSQAE 90

Query: 430  EDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFGDEGKDYMLE 489
              +   +   +D G E           +K    D   +  +   +   + G +  +    
Sbjct: 91   PSERRVKREKRDEGYE-------AAASSKASSGDASSLSIEETNKLRAKLGLKPLEVNAV 150

Query: 490  SDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLKKQTEE-- 549
                  ++  V   +++  + + +  + L+    A+    E+R+ N K  ++K   E+  
Sbjct: 151  KKEAGTKEEPV-AADVINPMALRQR-EELREKLAAAK---EKRLLNQKLGKIKTLGEDDP 210

Query: 550  --SEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVS-----DDDIAPEDTTN 609
               +  +W++RSR+L++    EK+ A + +K+ EE D  + GVS     + +   +D  +
Sbjct: 211  WLDDTAAWIERSRQLQK----EKDLAEKRAKLLEEMDQ-EFGVSTLVEEEFEQRRQDLYS 270

Query: 610  NHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYK 669
              DL G+ V H +D   EG  VVLTLKD+ +L +G      DVL NV + ++++ D   +
Sbjct: 271  ARDLQGLTVEHAIDSFREGETVVLTLKDKGVLQEGE-----DVLVNVNMVDKERADKNVE 330

Query: 670  AAKKKTGIYDDKFNDENYGE------KKMLPQYDDPADAD--EGLTLDGRGGFNNDAEKK 729
              KKK   Y     DE+  +      + +L +YD+  + +      L+  G  +   E++
Sbjct: 331  LRKKKPD-YLPYAEDESVDDLAQQKPRSILAKYDEELEGERPHSFRLEQGGMADGLRERE 390

Query: 730  LEELRRRLQGASSVKHFEDLN-VSTKVSHDYYTQDEMLKFKKPRKK-KSLRKKEKLDIDA 789
            LEE+R +L+        + LN V  +++ +Y + +EM+ FKK +++ K +RKKEK ++  
Sbjct: 391  LEEIRTKLR-----LQAQSLNTVGPRLASEYLSPEEMVTFKKTKRRVKKIRKKEK-EVIM 450

Query: 790  LEAEAISAG---LGVGDLGS--RNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRS 849
               + +  G      GD GS  R   RR+  + E+E  E E +    Q   +        
Sbjct: 451  RADDLLPLGEDQTQDGDFGSRLRGRGRRRVPEVEEEALEDEEKDPVAQPPPSDDTRVENM 510

Query: 850  LQLVQNSSARLEDNDDALIADDDE-DFYKSLERARKL-ALKKQDAASGPGAVALLATATT 909
                +     L      L  D+ E +  K LE+ R+L  L++       G   L      
Sbjct: 511  DISDEEDGGALPSGPPELEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVLEIVKKL 570

Query: 910  SSQATDDQSTKAGELQENKVVFTEMEEF--------VWGLQLDEDAHKPEEDDVFMD--- 969
             S+    +  +  E ++  +VF    EF         +GL     A   EE +  MD   
Sbjct: 571  ESRQRGWEEEEDPE-RKGTIVFNATSEFCRTLGEIPTYGL-----AGNREEQEELMDFER 630

Query: 970  DDEIPKEEYHEDVKDKDGGWTEVKDTAMEESTPEENEAVAPDETIHEVP-VGKGLSSALK 1029
            D+E       E   +++ GW+ V    ++E    ++ + +    + E P V +GL++AL 
Sbjct: 631  DEERSANGGSESDGEENIGWSTVN---LDEEKQHQDFSASSTTILDEEPIVNRGLAAALL 690

Query: 1030 LLKDRGTLKESIEWGGRNMDKRKS--KLVGIVDED---EPKESKSKDSR-----LSSLVD 1089
            L +++G L+ +++   R     KS    V  +++    + K S+ ++ R           
Sbjct: 691  LCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDG 750

Query: 1090 YKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNAD 1130
            YK ++ IE  DE GR +TPKE+FRQLSH+FHGKG GKMK E+RMK+  EE  LK+M ++D
Sbjct: 751  YKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSD 797

BLAST of CSPI05G31250.1 vs. ExPASy TrEMBL
Match: A0A0A0KXY6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650610 PE=3 SV=1)

HSP 1 Score: 1699.5 bits (4400), Expect = 0.0e+00
Identity = 936/940 (99.57%), Postives = 938/940 (99.79%), Query Frame = 0

Query: 243  MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 302
            MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS
Sbjct: 1    MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 60

Query: 303  KEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERERDRK 362
            KEKEKEAKDSERDR+RSREKRKEDRDEHEKERSRG KVKDKDYDRDIYKDKEYERERDRK
Sbjct: 61   KEKEKEAKDSERDRIRSREKRKEDRDEHEKERSRG-KVKDKDYDRDIYKDKEYERERDRK 120

Query: 363  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG 422
            DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG
Sbjct: 121  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG 180

Query: 423  REKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFGDE 482
            R+KHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGR GDE
Sbjct: 181  RDKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRIGDE 240

Query: 483  GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK 542
            GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK
Sbjct: 241  GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK 300

Query: 543  KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH 602
            KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH
Sbjct: 301  KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH 360

Query: 603  DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA 662
            DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA
Sbjct: 361  DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA 420

Query: 663  KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG 722
            KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG
Sbjct: 421  KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG 480

Query: 723  ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV 782
            ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV
Sbjct: 481  ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV 540

Query: 783  GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA 842
            GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA
Sbjct: 541  GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA 600

Query: 843  LIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQENK 902
            LIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQENK
Sbjct: 601  LIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQENK 660

Query: 903  VVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAMEE 962
            VVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAMEE
Sbjct: 661  VVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAMEE 720

Query: 963  STPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGIVD 1022
            STPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGIVD
Sbjct: 721  STPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGIVD 780

Query: 1023 EDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQE 1082
            EDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQE
Sbjct: 781  EDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQE 840

Query: 1083 KRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGFAT 1142
            KRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGFAT
Sbjct: 841  KRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGFAT 900

Query: 1143 VEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            VEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV
Sbjct: 901  VEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 939

BLAST of CSPI05G31250.1 vs. ExPASy TrEMBL
Match: A0A1S4E2I4 (SART-1 family protein DOT2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498705 PE=3 SV=1)

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 904/946 (95.56%), Postives = 922/946 (97.46%), Query Frame = 0

Query: 239  DSLNMDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSS 298
            +   MDWERSSAPDER+GDD GYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSS
Sbjct: 67   EHFQMDWERSSAPDERNGDDLGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSS 126

Query: 299  DDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERE 358
            DDASKEKEKE KDSERDRVRSREKRKEDRDEHEKER RGSKVKDKDYDR+IYKDKEYERE
Sbjct: 127  DDASKEKEKEVKDSERDRVRSREKRKEDRDEHEKERGRGSKVKDKDYDREIYKDKEYERE 186

Query: 359  RDRKDRGKDRERERERELEKDTVRGHDKERGKEKDRDRDK--DRDRDRKKKDKDKDRSNE 418
            RDRKDRGKDRERERERELEKD VRGHDKERGKEKDRDRDK  DRDRDRKKKDKDKDRSNE
Sbjct: 187  RDRKDRGKDRERERERELEKDNVRGHDKERGKEKDRDRDKDRDRDRDRKKKDKDKDRSNE 246

Query: 419  IEREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERI 478
            IEREKGREKHRDQEDKESYRN+DK+RGKERILEDDRKTDQ KQKLQDKEGIGSKNDEER 
Sbjct: 247  IEREKGREKHRDQEDKESYRNVDKERGKERILEDDRKTDQTKQKLQDKEGIGSKNDEERT 306

Query: 479  GRFGDEGKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNM 538
            G   DEGKDYMLESDGENNRDRDVNQGNMVQHLG EENFDGLKVGSH SSTMLEERIRNM
Sbjct: 307  GWIADEGKDYMLESDGENNRDRDVNQGNMVQHLGGEENFDGLKVGSHPSSTMLEERIRNM 366

Query: 539  KEDRLKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPE 598
            KEDRLKKQTEESEVL+WVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQ VSDDDIAPE
Sbjct: 367  KEDRLKKQTEESEVLAWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQDVSDDDIAPE 426

Query: 599  DTTNNHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRD 658
            +TTNNHDL GVKVLHGVDKVLEGGAVVLTLKDQSILADG+VNEELD+LENVEIGEQKQRD
Sbjct: 427  NTTNNHDLTGVKVLHGVDKVLEGGAVVLTLKDQSILADGDVNEELDMLENVEIGEQKQRD 486

Query: 659  IAYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEEL 718
            +AYKAAKKKTGIYDDKFNDEN GEKKMLPQYDDPA+ADEGLTLDGRGGFNNDAEKKLEEL
Sbjct: 487  MAYKAAKKKTGIYDDKFNDENDGEKKMLPQYDDPAEADEGLTLDGRGGFNNDAEKKLEEL 546

Query: 719  RRRLQGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAI 778
            RRRLQG SSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAI
Sbjct: 547  RRRLQGTSSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAI 606

Query: 779  SAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARL 838
            SAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQ SS RL
Sbjct: 607  SAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQTSSTRL 666

Query: 839  EDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAG 898
            EDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGA+ALLATATTSSQATDDQ+TKAG
Sbjct: 667  EDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAIALLATATTSSQATDDQNTKAG 726

Query: 899  ELQENKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVK 958
            ELQENKV+FTEMEEFVWGLQLDEDAHKPEE+DVFMDDDE+PKEEYHEDVKDKDGGWTEVK
Sbjct: 727  ELQENKVIFTEMEEFVWGLQLDEDAHKPEEEDVFMDDDEVPKEEYHEDVKDKDGGWTEVK 786

Query: 959  DTAMEESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSK 1018
            DTA EES P+EN+AVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSK
Sbjct: 787  DTAKEESIPDENKAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSK 846

Query: 1019 LVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGP 1078
            LVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGP
Sbjct: 847  LVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGP 906

Query: 1079 GKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDP 1138
            GKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDP
Sbjct: 907  GKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDP 966

Query: 1139 RSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            RSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV
Sbjct: 967  RSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1012

BLAST of CSPI05G31250.1 vs. ExPASy TrEMBL
Match: A0A1S3CAS5 (SART-1 family protein DOT2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498705 PE=3 SV=1)

HSP 1 Score: 1642.5 bits (4252), Expect = 0.0e+00
Identity = 904/942 (95.97%), Postives = 921/942 (97.77%), Query Frame = 0

Query: 243  MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 302
            MDWERSSAPDER+GDD GYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS
Sbjct: 1    MDWERSSAPDERNGDDLGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 60

Query: 303  KEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERERDRK 362
            KEKEKE KDSERDRVRSREKRKEDRDEHEKER RGSKVKDKDYDR+IYKDKEYERERDRK
Sbjct: 61   KEKEKEVKDSERDRVRSREKRKEDRDEHEKERGRGSKVKDKDYDREIYKDKEYERERDRK 120

Query: 363  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDK--DRDRDRKKKDKDKDRSNEIERE 422
            DRGKDRERERERELEKD VRGHDKERGKEKDRDRDK  DRDRDRKKKDKDKDRSNEIERE
Sbjct: 121  DRGKDRERERERELEKDNVRGHDKERGKEKDRDRDKDRDRDRDRKKKDKDKDRSNEIERE 180

Query: 423  KGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFG 482
            KGREKHRDQEDKESYRN+DK+RGKERILEDDRKTDQ KQKLQDKEGIGSKNDEER G   
Sbjct: 181  KGREKHRDQEDKESYRNVDKERGKERILEDDRKTDQTKQKLQDKEGIGSKNDEERTGWIA 240

Query: 483  DEGKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDR 542
            DEGKDYMLESDGENNRDRDVNQGNMVQHLG EENFDGLKVGSH SSTMLEERIRNMKEDR
Sbjct: 241  DEGKDYMLESDGENNRDRDVNQGNMVQHLGGEENFDGLKVGSHPSSTMLEERIRNMKEDR 300

Query: 543  LKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTN 602
            LKKQTEESEVL+WVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQ VSDDDIAPE+TTN
Sbjct: 301  LKKQTEESEVLAWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQDVSDDDIAPENTTN 360

Query: 603  NHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYK 662
            NHDL GVKVLHGVDKVLEGGAVVLTLKDQSILADG+VNEELD+LENVEIGEQKQRD+AYK
Sbjct: 361  NHDLTGVKVLHGVDKVLEGGAVVLTLKDQSILADGDVNEELDMLENVEIGEQKQRDMAYK 420

Query: 663  AAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRL 722
            AAKKKTGIYDDKFNDEN GEKKMLPQYDDPA+ADEGLTLDGRGGFNNDAEKKLEELRRRL
Sbjct: 421  AAKKKTGIYDDKFNDENDGEKKMLPQYDDPAEADEGLTLDGRGGFNNDAEKKLEELRRRL 480

Query: 723  QGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGL 782
            QG SSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGL
Sbjct: 481  QGTSSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGL 540

Query: 783  GVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDND 842
            GVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQ SS RLEDND
Sbjct: 541  GVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQTSSTRLEDND 600

Query: 843  DALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQE 902
            DALIADDDEDFYKSLERARKLALKKQDAASGPGA+ALLATATTSSQATDDQ+TKAGELQE
Sbjct: 601  DALIADDDEDFYKSLERARKLALKKQDAASGPGAIALLATATTSSQATDDQNTKAGELQE 660

Query: 903  NKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAM 962
            NKV+FTEMEEFVWGLQLDEDAHKPEE+DVFMDDDE+PKEEYHEDVKDKDGGWTEVKDTA 
Sbjct: 661  NKVIFTEMEEFVWGLQLDEDAHKPEEEDVFMDDDEVPKEEYHEDVKDKDGGWTEVKDTAK 720

Query: 963  EESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI 1022
            EES P+EN+AVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI
Sbjct: 721  EESIPDENKAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI 780

Query: 1023 VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK 1082
            VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK
Sbjct: 781  VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK 840

Query: 1083 QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF 1142
            QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF
Sbjct: 841  QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF 900

Query: 1143 ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV
Sbjct: 901  ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 942

BLAST of CSPI05G31250.1 vs. ExPASy TrEMBL
Match: A0A6J1FR42 (SART-1 family protein DOT2 OS=Cucurbita moschata OX=3662 GN=LOC111447438 PE=3 SV=1)

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 820/958 (85.59%), Postives = 872/958 (91.02%), Query Frame = 0

Query: 243  MDWERSSAP--DERSG----------DDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKD 302
            MD + SS P  DER+G          DDFG SGAEKSSKHRSEDHRKSSRGEEKDHRSKD
Sbjct: 1    MDADGSSGPEHDERNGHEARDRGEGQDDFGCSGAEKSSKHRSEDHRKSSRGEEKDHRSKD 60

Query: 303  RERSKRSSDDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIY 362
            R+RSKR SDDASKEKEKE KDSERDRV  RE+RKEDRDEH+KER+R  KVKDKDYDR++Y
Sbjct: 61   RDRSKRRSDDASKEKEKEVKDSERDRVHIRERRKEDRDEHDKERTREKKVKDKDYDREVY 120

Query: 363  KDKEYERERDRKDRGKDRERERERELEKDTVRGHDKERGKEKDRD------RDKDRDRDR 422
            K+KEYERERDRKDRGKD+ER RERELEKD VRG DKERGKEKDRD      R+++RDRDR
Sbjct: 121  KEKEYERERDRKDRGKDKERGRERELEKDNVRGQDKERGKEKDRDRERERERERERDRDR 180

Query: 423  KKKDKDKDRSNEIEREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDK 482
            KKK+KDKDRSNE EREKGREK RDQE+KESYRNIDKDRGKE+ L DD+K DQNK+KL+DK
Sbjct: 181  KKKEKDKDRSNENEREKGREKRRDQEEKESYRNIDKDRGKEKNLVDDKKGDQNKEKLRDK 240

Query: 483  EGIGSKNDEERIGRFGDEGKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHA 542
            EG G KN+EERI       KDYMLESDGE+NRDR V+QGN VQ LG EEN DGLKVG+ +
Sbjct: 241  EGTGGKNEEERIDWIAHGAKDYMLESDGEDNRDRGVDQGNAVQQLGGEENSDGLKVGAQS 300

Query: 543  SSTMLEERIRNMKEDRLKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNI 602
            SS MLEERIR MKEDRLKKQTEESEVL WVKRSRKLEEKKL+EKEKALQLSKIFEEQDNI
Sbjct: 301  SSAMLEERIRTMKEDRLKKQTEESEVLDWVKRSRKLEEKKLTEKEKALQLSKIFEEQDNI 360

Query: 603  DQGVSDDDIAPEDTTNNHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVL 662
            DQG SDDDIA ED T+N  LAGVKVLHG+DKVL GGAVVLTLKDQ+ILADG+VNE++DVL
Sbjct: 361  DQGASDDDIAAEDITSN--LAGVKVLHGIDKVLGGGAVVLTLKDQNILADGDVNEDMDVL 420

Query: 663  ENVEIGEQKQRDIAYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGG 722
            ENVEIGEQKQRD+AYKAAKKKTGIYDDKFNDEN GEKKMLPQYDDPA ADEGLTLDG G 
Sbjct: 421  ENVEIGEQKQRDMAYKAAKKKTGIYDDKFNDENDGEKKMLPQYDDPAAADEGLTLDGTGR 480

Query: 723  FNNDAEKKLEELRRRLQGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKE 782
            F+NDAEKKLEELR+RLQGASSVKHFEDLN S KVSHDYYTQDEML+FKKP+KKKSLRKKE
Sbjct: 481  FSNDAEKKLEELRKRLQGASSVKHFEDLNASVKVSHDYYTQDEMLRFKKPKKKKSLRKKE 540

Query: 783  KLDIDALEAEAISAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASR 842
            KLDIDALEAEAIS+GLGVGDLGSRNDS RQA+K EQE+SEAEMR NAYQSAYAKADEASR
Sbjct: 541  KLDIDALEAEAISSGLGVGDLGSRNDSSRQARKTEQERSEAEMRQNAYQSAYAKADEASR 600

Query: 843  SLQLVQNSSARLEDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTS 902
            SLQLVQ SS RL+DN+D LI DDDED YKSLERARKLALKKQ+AASGP AVALLAT TTS
Sbjct: 601  SLQLVQ-SSVRLDDNEDTLIEDDDEDLYKSLERARKLALKKQEAASGPEAVALLATTTTS 660

Query: 903  SQATDDQSTKAGELQENKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHED 962
             Q TDDQ+TKAGELQENKVVFTEMEEFVWGLQLDE++HKPEE+DVFMDDDE PKEEYHED
Sbjct: 661  GQTTDDQNTKAGELQENKVVFTEMEEFVWGLQLDEESHKPEEEDVFMDDDEAPKEEYHED 720

Query: 963  VKDKDGGWTEVKDTAMEESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIE 1022
             KDKDGGWTEVKDTA EE TPE+NE +APDETIHEVPVGKGLSS LKLLKDRGTLKESIE
Sbjct: 721  EKDKDGGWTEVKDTAKEEPTPEDNETIAPDETIHEVPVGKGLSSVLKLLKDRGTLKESIE 780

Query: 1023 WGGRNMDKRKSKLVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESF 1082
            WGGRNMDKRKSKLVGI+DEDEPKE+KSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESF
Sbjct: 781  WGGRNMDKRKSKLVGIIDEDEPKEAKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESF 840

Query: 1083 RQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVL 1142
            RQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVL
Sbjct: 841  RQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVL 900

Query: 1143 SGHVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            SGHVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKG+ SNTGTKK K+
Sbjct: 901  SGHVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGDPSNTGTKKPKI 955

BLAST of CSPI05G31250.1 vs. ExPASy TrEMBL
Match: A0A6J1IPE4 (SART-1 family protein DOT2 OS=Cucurbita maxima OX=3661 GN=LOC111478749 PE=3 SV=1)

HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 815/956 (85.25%), Postives = 870/956 (91.00%), Query Frame = 0

Query: 243  MDWERSSAP--DERSG----------DDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKD 302
            MD + SS P  DER+G          DDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKD
Sbjct: 1    MDADGSSVPEHDERNGHEARDRGEGQDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKD 60

Query: 303  RERSKRSSDDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIY 362
            R+RSKR SDDASKEKEKE KDSERDRV  RE+RKEDRDEH+KER+R  KVKDKDYDR++Y
Sbjct: 61   RDRSKRRSDDASKEKEKEVKDSERDRVHIRERRKEDRDEHDKERTREKKVKDKDYDREVY 120

Query: 363  KDKEYERERDRKDRGKDRERERERELEKDTVRGHDKERGKEKDRD--RDKDRDRDRKKKD 422
            K+KEY+RERDRKDRGKD+ER RERELEKD VRG DKERGKEKDRD  R+++RDRDRKKK+
Sbjct: 121  KEKEYDRERDRKDRGKDKERGRERELEKDNVRGQDKERGKEKDRDRERERERDRDRKKKE 180

Query: 423  KDKDRSNEIEREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIG 482
            KDKDRSNE EREKGREK RDQE+KESYRNIDKDRGKE+ L DD+K DQNK+KL+DKEGIG
Sbjct: 181  KDKDRSNENEREKGREKRRDQEEKESYRNIDKDRGKEKNLVDDKKGDQNKEKLRDKEGIG 240

Query: 483  SKNDEERIGRFGDEGKDYML--ESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASS 542
             KNDEERI        D++   ESDGE+NRDR V+QGN VQHLG E+N DGLKVG+ +SS
Sbjct: 241  GKNDEERI--------DWLAHGESDGEDNRDRGVDQGNAVQHLGGEDNSDGLKVGAQSSS 300

Query: 543  TMLEERIRNMKEDRLKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQ 602
             MLEERIR MKEDRLKKQTEESEVL WVKRSRKLEEKKL+EKEKALQLSKIFEEQDNIDQ
Sbjct: 301  AMLEERIRTMKEDRLKKQTEESEVLDWVKRSRKLEEKKLTEKEKALQLSKIFEEQDNIDQ 360

Query: 603  GVSDDDIAPEDTTNNHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLEN 662
            G SDDDIA ED T+N  LAGVKVLHG+DKVL GGAVVLTLKDQ+ILADG+VNE++DVLEN
Sbjct: 361  GASDDDIAAEDITSN--LAGVKVLHGIDKVLGGGAVVLTLKDQNILADGDVNEDMDVLEN 420

Query: 663  VEIGEQKQRDIAYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFN 722
            VEIGEQKQRD+AYKAAKKKTGIYDDKFNDEN GEKKMLPQYDDPA ADEGLTLDG G F+
Sbjct: 421  VEIGEQKQRDMAYKAAKKKTGIYDDKFNDENDGEKKMLPQYDDPAAADEGLTLDGTGRFS 480

Query: 723  NDAEKKLEELRRRLQGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKL 782
            NDAEKKLEELR+RLQGASSVKHFEDLN S KVSHDYYTQDEML+FKKP+KKKSLRKKEKL
Sbjct: 481  NDAEKKLEELRKRLQGASSVKHFEDLNASVKVSHDYYTQDEMLRFKKPKKKKSLRKKEKL 540

Query: 783  DIDALEAEAISAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSL 842
            DIDALEAEAIS+GLGVGDLGSRNDS RQA+K EQE+SEAEMR NAYQSAYAKADEASRSL
Sbjct: 541  DIDALEAEAISSGLGVGDLGSRNDSSRQARKTEQERSEAEMRQNAYQSAYAKADEASRSL 600

Query: 843  QLVQNSSARLEDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQ 902
            QLVQ SS RL+ N+D LI DDDED YKSLERARKLALKKQ+AASGP AVALLAT TTS Q
Sbjct: 601  QLVQ-SSVRLDGNEDTLIEDDDEDLYKSLERARKLALKKQEAASGPEAVALLATTTTSGQ 660

Query: 903  ATDDQSTKAGELQENKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVK 962
             TDDQ+TKAGELQENKVVFTEMEEFVWGLQLDE++HKPEE+DVFMDDDE PKEEYHED K
Sbjct: 661  TTDDQNTKAGELQENKVVFTEMEEFVWGLQLDEESHKPEEEDVFMDDDEAPKEEYHEDEK 720

Query: 963  DKDGGWTEVKDTAMEESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWG 1022
            DKDGGWTEVKDTA EE  PE+NE +APDETIHEVPVGKGLSS LKLLKDRGTLKESIEWG
Sbjct: 721  DKDGGWTEVKDTAKEEPAPEDNETIAPDETIHEVPVGKGLSSVLKLLKDRGTLKESIEWG 780

Query: 1023 GRNMDKRKSKLVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQ 1082
            GRNMDKRKSKLVGI+DEDEPKE+KSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQ
Sbjct: 781  GRNMDKRKSKLVGIIDEDEPKEAKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQ 840

Query: 1083 LSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSG 1142
            LSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSG
Sbjct: 841  LSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSG 900

Query: 1143 HVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            HVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKG+  NTGTKK K+
Sbjct: 901  HVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGDPLNTGTKKPKI 945

BLAST of CSPI05G31250.1 vs. NCBI nr
Match: XP_011656109.1 (SART-1 family protein DOT2 [Cucumis sativus] >XP_011656110.1 SART-1 family protein DOT2 [Cucumis sativus] >XP_031741040.1 SART-1 family protein DOT2 [Cucumis sativus] >XP_031741041.1 SART-1 family protein DOT2 [Cucumis sativus] >XP_031741042.1 SART-1 family protein DOT2 [Cucumis sativus] >XP_031741043.1 SART-1 family protein DOT2 [Cucumis sativus] >XP_031741044.1 SART-1 family protein DOT2 [Cucumis sativus] >KGN52696.1 hypothetical protein Csa_008281 [Cucumis sativus])

HSP 1 Score: 1699.5 bits (4400), Expect = 0.0e+00
Identity = 936/940 (99.57%), Postives = 938/940 (99.79%), Query Frame = 0

Query: 243  MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 302
            MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS
Sbjct: 1    MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 60

Query: 303  KEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERERDRK 362
            KEKEKEAKDSERDR+RSREKRKEDRDEHEKERSRG KVKDKDYDRDIYKDKEYERERDRK
Sbjct: 61   KEKEKEAKDSERDRIRSREKRKEDRDEHEKERSRG-KVKDKDYDRDIYKDKEYERERDRK 120

Query: 363  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG 422
            DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG
Sbjct: 121  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG 180

Query: 423  REKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFGDE 482
            R+KHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGR GDE
Sbjct: 181  RDKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRIGDE 240

Query: 483  GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK 542
            GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK
Sbjct: 241  GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK 300

Query: 543  KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH 602
            KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH
Sbjct: 301  KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH 360

Query: 603  DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA 662
            DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA
Sbjct: 361  DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA 420

Query: 663  KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG 722
            KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG
Sbjct: 421  KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG 480

Query: 723  ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV 782
            ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV
Sbjct: 481  ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV 540

Query: 783  GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA 842
            GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA
Sbjct: 541  GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA 600

Query: 843  LIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQENK 902
            LIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQENK
Sbjct: 601  LIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQENK 660

Query: 903  VVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAMEE 962
            VVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAMEE
Sbjct: 661  VVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAMEE 720

Query: 963  STPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGIVD 1022
            STPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGIVD
Sbjct: 721  STPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGIVD 780

Query: 1023 EDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQE 1082
            EDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQE
Sbjct: 781  EDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQE 840

Query: 1083 KRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGFAT 1142
            KRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGFAT
Sbjct: 841  KRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGFAT 900

Query: 1143 VEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            VEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV
Sbjct: 901  VEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 939

BLAST of CSPI05G31250.1 vs. NCBI nr
Match: XP_016902433.1 (PREDICTED: SART-1 family protein DOT2 isoform X1 [Cucumis melo])

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 904/946 (95.56%), Postives = 922/946 (97.46%), Query Frame = 0

Query: 239  DSLNMDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSS 298
            +   MDWERSSAPDER+GDD GYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSS
Sbjct: 67   EHFQMDWERSSAPDERNGDDLGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSS 126

Query: 299  DDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERE 358
            DDASKEKEKE KDSERDRVRSREKRKEDRDEHEKER RGSKVKDKDYDR+IYKDKEYERE
Sbjct: 127  DDASKEKEKEVKDSERDRVRSREKRKEDRDEHEKERGRGSKVKDKDYDREIYKDKEYERE 186

Query: 359  RDRKDRGKDRERERERELEKDTVRGHDKERGKEKDRDRDK--DRDRDRKKKDKDKDRSNE 418
            RDRKDRGKDRERERERELEKD VRGHDKERGKEKDRDRDK  DRDRDRKKKDKDKDRSNE
Sbjct: 187  RDRKDRGKDRERERERELEKDNVRGHDKERGKEKDRDRDKDRDRDRDRKKKDKDKDRSNE 246

Query: 419  IEREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERI 478
            IEREKGREKHRDQEDKESYRN+DK+RGKERILEDDRKTDQ KQKLQDKEGIGSKNDEER 
Sbjct: 247  IEREKGREKHRDQEDKESYRNVDKERGKERILEDDRKTDQTKQKLQDKEGIGSKNDEERT 306

Query: 479  GRFGDEGKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNM 538
            G   DEGKDYMLESDGENNRDRDVNQGNMVQHLG EENFDGLKVGSH SSTMLEERIRNM
Sbjct: 307  GWIADEGKDYMLESDGENNRDRDVNQGNMVQHLGGEENFDGLKVGSHPSSTMLEERIRNM 366

Query: 539  KEDRLKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPE 598
            KEDRLKKQTEESEVL+WVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQ VSDDDIAPE
Sbjct: 367  KEDRLKKQTEESEVLAWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQDVSDDDIAPE 426

Query: 599  DTTNNHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRD 658
            +TTNNHDL GVKVLHGVDKVLEGGAVVLTLKDQSILADG+VNEELD+LENVEIGEQKQRD
Sbjct: 427  NTTNNHDLTGVKVLHGVDKVLEGGAVVLTLKDQSILADGDVNEELDMLENVEIGEQKQRD 486

Query: 659  IAYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEEL 718
            +AYKAAKKKTGIYDDKFNDEN GEKKMLPQYDDPA+ADEGLTLDGRGGFNNDAEKKLEEL
Sbjct: 487  MAYKAAKKKTGIYDDKFNDENDGEKKMLPQYDDPAEADEGLTLDGRGGFNNDAEKKLEEL 546

Query: 719  RRRLQGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAI 778
            RRRLQG SSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAI
Sbjct: 547  RRRLQGTSSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAI 606

Query: 779  SAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARL 838
            SAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQ SS RL
Sbjct: 607  SAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQTSSTRL 666

Query: 839  EDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAG 898
            EDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGA+ALLATATTSSQATDDQ+TKAG
Sbjct: 667  EDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAIALLATATTSSQATDDQNTKAG 726

Query: 899  ELQENKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVK 958
            ELQENKV+FTEMEEFVWGLQLDEDAHKPEE+DVFMDDDE+PKEEYHEDVKDKDGGWTEVK
Sbjct: 727  ELQENKVIFTEMEEFVWGLQLDEDAHKPEEEDVFMDDDEVPKEEYHEDVKDKDGGWTEVK 786

Query: 959  DTAMEESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSK 1018
            DTA EES P+EN+AVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSK
Sbjct: 787  DTAKEESIPDENKAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSK 846

Query: 1019 LVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGP 1078
            LVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGP
Sbjct: 847  LVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGP 906

Query: 1079 GKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDP 1138
            GKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDP
Sbjct: 907  GKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDP 966

Query: 1139 RSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            RSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV
Sbjct: 967  RSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1012

BLAST of CSPI05G31250.1 vs. NCBI nr
Match: XP_008459644.1 (PREDICTED: SART-1 family protein DOT2 isoform X2 [Cucumis melo] >XP_008459646.1 PREDICTED: SART-1 family protein DOT2 isoform X2 [Cucumis melo] >XP_008459647.1 PREDICTED: SART-1 family protein DOT2 isoform X2 [Cucumis melo] >XP_008459648.1 PREDICTED: SART-1 family protein DOT2 isoform X2 [Cucumis melo])

HSP 1 Score: 1642.5 bits (4252), Expect = 0.0e+00
Identity = 904/942 (95.97%), Postives = 921/942 (97.77%), Query Frame = 0

Query: 243  MDWERSSAPDERSGDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 302
            MDWERSSAPDER+GDD GYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS
Sbjct: 1    MDWERSSAPDERNGDDLGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKDRERSKRSSDDAS 60

Query: 303  KEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERERDRK 362
            KEKEKE KDSERDRVRSREKRKEDRDEHEKER RGSKVKDKDYDR+IYKDKEYERERDRK
Sbjct: 61   KEKEKEVKDSERDRVRSREKRKEDRDEHEKERGRGSKVKDKDYDREIYKDKEYERERDRK 120

Query: 363  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDK--DRDRDRKKKDKDKDRSNEIERE 422
            DRGKDRERERERELEKD VRGHDKERGKEKDRDRDK  DRDRDRKKKDKDKDRSNEIERE
Sbjct: 121  DRGKDRERERERELEKDNVRGHDKERGKEKDRDRDKDRDRDRDRKKKDKDKDRSNEIERE 180

Query: 423  KGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFG 482
            KGREKHRDQEDKESYRN+DK+RGKERILEDDRKTDQ KQKLQDKEGIGSKNDEER G   
Sbjct: 181  KGREKHRDQEDKESYRNVDKERGKERILEDDRKTDQTKQKLQDKEGIGSKNDEERTGWIA 240

Query: 483  DEGKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDR 542
            DEGKDYMLESDGENNRDRDVNQGNMVQHLG EENFDGLKVGSH SSTMLEERIRNMKEDR
Sbjct: 241  DEGKDYMLESDGENNRDRDVNQGNMVQHLGGEENFDGLKVGSHPSSTMLEERIRNMKEDR 300

Query: 543  LKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTN 602
            LKKQTEESEVL+WVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQ VSDDDIAPE+TTN
Sbjct: 301  LKKQTEESEVLAWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQDVSDDDIAPENTTN 360

Query: 603  NHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYK 662
            NHDL GVKVLHGVDKVLEGGAVVLTLKDQSILADG+VNEELD+LENVEIGEQKQRD+AYK
Sbjct: 361  NHDLTGVKVLHGVDKVLEGGAVVLTLKDQSILADGDVNEELDMLENVEIGEQKQRDMAYK 420

Query: 663  AAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRL 722
            AAKKKTGIYDDKFNDEN GEKKMLPQYDDPA+ADEGLTLDGRGGFNNDAEKKLEELRRRL
Sbjct: 421  AAKKKTGIYDDKFNDENDGEKKMLPQYDDPAEADEGLTLDGRGGFNNDAEKKLEELRRRL 480

Query: 723  QGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGL 782
            QG SSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGL
Sbjct: 481  QGTSSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGL 540

Query: 783  GVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDND 842
            GVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQ SS RLEDND
Sbjct: 541  GVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQTSSTRLEDND 600

Query: 843  DALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDDQSTKAGELQE 902
            DALIADDDEDFYKSLERARKLALKKQDAASGPGA+ALLATATTSSQATDDQ+TKAGELQE
Sbjct: 601  DALIADDDEDFYKSLERARKLALKKQDAASGPGAIALLATATTSSQATDDQNTKAGELQE 660

Query: 903  NKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAM 962
            NKV+FTEMEEFVWGLQLDEDAHKPEE+DVFMDDDE+PKEEYHEDVKDKDGGWTEVKDTA 
Sbjct: 661  NKVIFTEMEEFVWGLQLDEDAHKPEEEDVFMDDDEVPKEEYHEDVKDKDGGWTEVKDTAK 720

Query: 963  EESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI 1022
            EES P+EN+AVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI
Sbjct: 721  EESIPDENKAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI 780

Query: 1023 VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK 1082
            VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK
Sbjct: 781  VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK 840

Query: 1083 QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF 1142
            QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF
Sbjct: 841  QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF 900

Query: 1143 ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV
Sbjct: 901  ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 942

BLAST of CSPI05G31250.1 vs. NCBI nr
Match: XP_038890025.1 (SART-1 family protein DOT2 [Benincasa hispida] >XP_038890026.1 SART-1 family protein DOT2 [Benincasa hispida])

HSP 1 Score: 1590.1 bits (4116), Expect = 0.0e+00
Identity = 878/958 (91.65%), Postives = 918/958 (95.82%), Query Frame = 0

Query: 243  MDWERSSAPDERSG--------------DDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRS 302
            MD ERSSAPDER+G              DDFGYSG EKSSKHRSEDHRKSSRGEEKDHRS
Sbjct: 1    MDGERSSAPDERNGPDIAWARERGEGGHDDFGYSGGEKSSKHRSEDHRKSSRGEEKDHRS 60

Query: 303  KDRERSKRSSDDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRD 362
            KDRERSKRSSDDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDR+
Sbjct: 61   KDRERSKRSSDDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRE 120

Query: 363  IYKDKEYERERDRKDRGKDRERERERELEKDTVRGHDKERGKE----KDRDRDKDRDRDR 422
            IYK+KEYERERDRKDRGKDRERERERELEKD VRG DKE+GKE    +DRDRD+DRDRDR
Sbjct: 121  IYKEKEYERERDRKDRGKDRERERERELEKDNVRGQDKEKGKERDRDRDRDRDRDRDRDR 180

Query: 423  KKKDKDKDRSNEIEREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDK 482
            KKKDKDKDRSNE EREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQ+K+K QDK
Sbjct: 181  KKKDKDKDRSNENEREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQSKEKSQDK 240

Query: 483  EGIGSKNDEERIGRFGDEGKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHA 542
            EGIGSK DEERIG   DEGKDYM+ESDG+NNR+RDV+QGNMVQ LG EENFDGLKVG+HA
Sbjct: 241  EGIGSKIDEERIGWIADEGKDYMVESDGDNNRNRDVDQGNMVQDLGGEENFDGLKVGAHA 300

Query: 543  SSTMLEERIRNMKEDRLKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNI 602
            SSTMLEERIRNMKEDRLKKQTEESEVL+WVKRSRKLEEKKLSEKEKALQLSKIFEEQDNI
Sbjct: 301  SSTMLEERIRNMKEDRLKKQTEESEVLAWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNI 360

Query: 603  DQGVSDDDIAPEDTTNNHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVL 662
            DQGVSDDDIAPEDTTNNH+LAGVKVLHG+DKVLEGGAVVLTLKDQ+ILADG++NE++DVL
Sbjct: 361  DQGVSDDDIAPEDTTNNHNLAGVKVLHGIDKVLEGGAVVLTLKDQNILADGDINEDVDVL 420

Query: 663  ENVEIGEQKQRDIAYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGG 722
            ENVEIGEQKQRD+AYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPA ADEGLTLDGRGG
Sbjct: 421  ENVEIGEQKQRDMAYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPAAADEGLTLDGRGG 480

Query: 723  FNNDAEKKLEELRRRLQGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKE 782
            FNNDAEKKLEELRRRLQG++SV HFEDLN STKVSHDYYTQDEMLKFKKPRKKKSLRKKE
Sbjct: 481  FNNDAEKKLEELRRRLQGSNSVMHFEDLNASTKVSHDYYTQDEMLKFKKPRKKKSLRKKE 540

Query: 783  KLDIDALEAEAISAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASR 842
            KLD+DALEAEAISAGLGVGDLGSRN SRRQA+KEEQEKSEAEMR NAYQSAYAKADEASR
Sbjct: 541  KLDLDALEAEAISAGLGVGDLGSRNGSRRQAQKEEQEKSEAEMRHNAYQSAYAKADEASR 600

Query: 843  SLQLVQNSSARLEDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTS 902
            SL+LVQ SS RLEDNDD LIADDDEDFYKSL+RARKLALK+Q+AASGPGA+ALLATATTS
Sbjct: 601  SLKLVQTSSVRLEDNDDTLIADDDEDFYKSLDRARKLALKRQEAASGPGAIALLATATTS 660

Query: 903  SQATDDQSTKAGELQENKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHED 962
            SQ TDDQ+TKAGELQENKVVFTEMEEFVWGLQLDEDAHKPEE+DVFMDDDE+PKEEYHE+
Sbjct: 661  SQTTDDQNTKAGELQENKVVFTEMEEFVWGLQLDEDAHKPEEEDVFMDDDEVPKEEYHEE 720

Query: 963  VKDKDGGWTEVKDTAMEESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIE 1022
            +KDKDGGWTEVKDTA EE+TPEENEA+APDETIHEVPVGKGLSSALKLLK+RGTLKESIE
Sbjct: 721  IKDKDGGWTEVKDTAKEETTPEENEAIAPDETIHEVPVGKGLSSALKLLKERGTLKESIE 780

Query: 1023 WGGRNMDKRKSKLVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESF 1082
            WGGRNMDKRKSKLVGIVDEDEPKESKSK+SRLSSLVDYKKEIHIERTDEFGRIMTPKESF
Sbjct: 781  WGGRNMDKRKSKLVGIVDEDEPKESKSKESRLSSLVDYKKEIHIERTDEFGRIMTPKESF 840

Query: 1083 RQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVL 1142
            RQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVL
Sbjct: 841  RQLSHKFHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVL 900

Query: 1143 SGHVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            SGHVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV
Sbjct: 901  SGHVKPGQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 958

BLAST of CSPI05G31250.1 vs. NCBI nr
Match: KAG7030981.1 (SART-1 family protein DOT2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 823/952 (86.45%), Postives = 875/952 (91.91%), Query Frame = 0

Query: 243  MDWERSSAP--DERSG----------DDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKD 302
            MD + SS P  DER+G          DDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKD
Sbjct: 1    MDADGSSVPEHDERNGHEARDRGEGQDDFGYSGAEKSSKHRSEDHRKSSRGEEKDHRSKD 60

Query: 303  RERSKRSSDDASKEKEKEAKDSERDRVRSREKRKEDRDEHEKERSRGSKVKDKDYDRDIY 362
            R+RSKR SDDASKEKEKE KDSERDRV  RE+RKEDRDEH+KER+R  KVKDKDYDR++Y
Sbjct: 61   RDRSKRRSDDASKEKEKEVKDSERDRVHIRERRKEDRDEHDKERTREKKVKDKDYDREVY 120

Query: 363  KDKEYERERDRKDRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKD 422
            K+KEYERERDRKDRGKD+ER RERELEKD VRG DKERGKEKDRDR+++RDRDRKKK+KD
Sbjct: 121  KEKEYERERDRKDRGKDKERGRERELEKDNVRGQDKERGKEKDRDRERERDRDRKKKEKD 180

Query: 423  KDRSNEIEREKGREKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSK 482
            KDRSNE EREKGREK RDQE+KESYRNIDK+RGKE+ L DD+K DQNK+KL+DKEGIG K
Sbjct: 181  KDRSNENEREKGREKRRDQEEKESYRNIDKERGKEKNLVDDKKGDQNKEKLRDKEGIGGK 240

Query: 483  NDEERIGRFGDEGKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLE 542
            NDEERI       KDYMLESDGE+NRDR V+QGN VQHLG EEN DGLKVG+ +SS MLE
Sbjct: 241  NDEERIDWIAHGAKDYMLESDGEDNRDRGVDQGNAVQHLGGEENSDGLKVGAQSSSAMLE 300

Query: 543  ERIRNMKEDRLKKQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSD 602
            ERIR MKEDRLKKQTEESEVL WVKRSRKLEEKKL+EKEKALQLSKIFEEQDNIDQG SD
Sbjct: 301  ERIRTMKEDRLKKQTEESEVLDWVKRSRKLEEKKLTEKEKALQLSKIFEEQDNIDQGASD 360

Query: 603  DDIAPEDTTNNHDLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIG 662
            DDIA ED T+N  LAGVKVLHG+DKVL GGAVVLTLKDQ+ILADG+VNE++DVLENVEIG
Sbjct: 361  DDIAAEDITSN--LAGVKVLHGIDKVLGGGAVVLTLKDQNILADGDVNEDMDVLENVEIG 420

Query: 663  EQKQRDIAYKAAKKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAE 722
            EQKQRD+AYKAAKKKTGIYDDKFNDEN GEKKMLPQYDDPA ADEGLTLDG G F+NDAE
Sbjct: 421  EQKQRDMAYKAAKKKTGIYDDKFNDENDGEKKMLPQYDDPAAADEGLTLDGTGRFSNDAE 480

Query: 723  KKLEELRRRLQGASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDA 782
            KKLEELR+RLQGASSVKHFEDLN S KVSHDYYTQDEML+FKKP+KKKSLRKKEKLDIDA
Sbjct: 481  KKLEELRKRLQGASSVKHFEDLNASVKVSHDYYTQDEMLRFKKPKKKKSLRKKEKLDIDA 540

Query: 783  LEAEAISAGLGVGDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQ 842
            LEAEAIS+GLGVGDLGSRNDS RQA+K EQE+SEAEMR NAYQSAYAKADEASRSLQLVQ
Sbjct: 541  LEAEAISSGLGVGDLGSRNDSSRQARKTEQERSEAEMRQNAYQSAYAKADEASRSLQLVQ 600

Query: 843  NSSARLEDNDDALIADDDEDFYKSLERARKLALKKQDAASGPGAVALLATATTSSQATDD 902
             SS RL+DN+D LI DDDED YKSLERARKLALKKQ+AASGP AVALLAT TTS Q TDD
Sbjct: 601  -SSVRLDDNEDTLIEDDDEDLYKSLERARKLALKKQEAASGPEAVALLATTTTSGQTTDD 660

Query: 903  QSTKAGELQENKVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDG 962
            Q+TKAGELQENKVVFTEMEEFVWGLQLDE++HKPEE+DVFMDDDE PKEEYHED KDKDG
Sbjct: 661  QNTKAGELQENKVVFTEMEEFVWGLQLDEESHKPEEEDVFMDDDEAPKEEYHEDEKDKDG 720

Query: 963  GWTEVKDTAMEESTPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNM 1022
            GWTEVKDTA EE TPE+NE +APDETIHEVPVGKGLSS LKLLKDRGTLKESIEWGGRNM
Sbjct: 721  GWTEVKDTAKEEPTPEDNETIAPDETIHEVPVGKGLSSVLKLLKDRGTLKESIEWGGRNM 780

Query: 1023 DKRKSKLVGIVDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHK 1082
            DKRKSKLVGI+DEDEPKE+KSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHK
Sbjct: 781  DKRKSKLVGIIDEDEPKEAKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHK 840

Query: 1083 FHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKP 1142
            FHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKP
Sbjct: 841  FHGKGPGKMKQEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKP 900

Query: 1143 GQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGTKKAKV 1183
            GQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKG+ SNTGTKK K+
Sbjct: 901  GQTSDPRSGFATVEKDLPGGLTPMLGDRKVEHFLGIKRKGDPSNTGTKKPKI 949

BLAST of CSPI05G31250.1 vs. TAIR 10
Match: AT5G16780.1 (SART-1 family )

HSP 1 Score: 783.5 bits (2022), Expect = 2.4e-226
Identity = 491/877 (55.99%), Postives = 625/877 (71.27%), Query Frame = 0

Query: 311  DSERDRVRSREKR--------KEDRDEHEKERSRGSKVKDKDYDRDIYKDKEYERERDRK 370
            +  + R   RE+R        +E RD   KE+   SK K+KDYDR+  +DK++ R+   K
Sbjct: 4    EKSKSRHEIREERADYEGSPVREHRDGRRKEKDHRSKDKEKDYDREKIRDKDHRRD---K 63

Query: 371  DRGKDRERERERELEKDTVRGHDKERGKEKDRDRDKDRDRDRKKKDKDKDRSNEIEREKG 430
            ++ +DR+R R+ + EK+  RG DKER K+K RDR K++D++ K++++ KDR NE + EK 
Sbjct: 64   EKERDRKRSRDEDTEKEISRGRDKEREKDKSRDRVKEKDKE-KERNRHKDRENERDNEKE 123

Query: 431  REKHRDQEDKESYRNIDKDRGKERILEDDRKTDQNKQKLQDKEGIGSKNDEERIGRFGDE 490
            ++K R +          K+R  ++  EDD +T +  ++                      
Sbjct: 124  KDKDRARV---------KERASKKSHEDDDETHKAAERY--------------------- 183

Query: 491  GKDYMLESDGENNRDRDVNQGNMVQHLGVEENFDGLKVGSHASSTMLEERIRNMKEDRLK 550
                      E++ +R +N+G         +N D    G  AS+  L+ RI  M+E+R K
Sbjct: 184  ----------EHSDNRGLNEGG--------DNVDAASSGKEASALDLQNRILKMREERKK 243

Query: 551  KQTEESEVLSWVKRSRKLEEKKLSEKEKALQLSKIFEEQDNIDQGVSDDDIAPEDTTNNH 610
            K  + S+ LSWV RSRK+EEK+ +EK++A QLS+IFEEQDN++QG +      ED  +  
Sbjct: 244  KAEDASDALSWVARSRKIEEKRNAEKQRAQQLSRIFEEQDNLNQGEN------EDGEDGE 303

Query: 611  DLAGVKVLHGVDKVLEGGAVVLTLKDQSILADGNVNEELDVLENVEIGEQKQRDIAYKAA 670
             L+GVKVLHG++KV+EGGAV+LTLKDQS+L DG+VN E+D+LENVEIGEQK+R+ AY+AA
Sbjct: 304  HLSGVKVLHGLEKVVEGGAVILTLKDQSVLTDGDVNNEIDMLENVEIGEQKRRNEAYEAA 363

Query: 671  KKKTGIYDDKFNDENYGEKKMLPQYDDPADADEGLTLDGRGGFNNDAEKKLEELRRRLQG 730
            KKK GIYDDKFND+   EKKMLPQYD+ A  DEG+ LD +G F  +AEKKLEELR+R+QG
Sbjct: 364  KKKKGIYDDKFNDDPGAEKKMLPQYDEAA-TDEGIFLDAKGRFTGEAEKKLEELRKRIQG 423

Query: 731  ASSVKHFEDLNVSTKVSHDYYTQDEMLKFKKPRKKKSLRKKEKLDIDALEAEAISAGLGV 790
              +   FEDLN S KVS DY++Q+EMLKFKKP+KKK LRKK+KLD+  LEAEA+++GLG 
Sbjct: 424  -QTTHTFEDLNSSAKVSSDYFSQEEMLKFKKPKKKKQLRKKDKLDLSMLEAEAVASGLGA 483

Query: 791  GDLGSRNDSRRQAKKEEQEKSEAEMRLNAYQSAYAKADEASRSLQLVQNSSARLEDNDDA 850
             DLGSR D RRQA KEE+E+ E E R NAYQ A AKADEASR L+  Q    + ++++  
Sbjct: 484  EDLGSRKDGRRQAMKEEKERIEYEKRSNAYQEAIAKADEASRLLRREQVQPFKRDEDESM 543

Query: 851  LIADDDEDFYKSLERARKLAL-KKQDAASGPGAVALLATATTSSQATDDQSTKAGELQEN 910
            ++ADD ED YKSLE+AR+LAL KK++A SGP AVA L  A++++Q TDD +T   E QEN
Sbjct: 544  VLADDAEDLYKSLEKARRLALIKKEEAGSGPQAVAHL-VASSTNQTTDDNTTTGDETQEN 603

Query: 911  KVVFTEMEEFVWGLQLDEDAHKPEEDDVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAME 970
             VVFTEM +FVWGLQ + D  KPE +DVFM++D  PK    E  ++   G TEV DT M+
Sbjct: 604  TVVFTEMGDFVWGLQRENDVRKPESEDVFMEEDVAPKAPV-EVKEEHPDGLTEVNDTDMD 663

Query: 971  ES-TPEENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGI 1030
             +    + + + PDE IHEV VGKGLS ALKLLKDRGTLKE +EWGGRNMDK+KSKLVGI
Sbjct: 664  AAEDSSDTKEITPDENIHEVAVGKGLSGALKLLKDRGTLKEKVEWGGRNMDKKKSKLVGI 723

Query: 1031 VDEDEPKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMK 1090
            VD+D  KESK K+S+     D  K+I IERTDEFGR +TPKE+FR LSHKFHGKGPGKMK
Sbjct: 724  VDDDGGKESKDKESK-----DRFKDIRIERTDEFGRTLTPKEAFRLLSHKFHGKGPGKMK 783

Query: 1091 QEKRMKQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVLSGHVKPGQTSDPRSGF 1150
            +EKRMKQYQEELKLKQMKN+DTPS SV+RMREAQAQLKTPYLVLSGHVKPGQTSDP+SGF
Sbjct: 784  EEKRMKQYQEELKLKQMKNSDTPSQSVQRMREAQAQLKTPYLVLSGHVKPGQTSDPQSGF 813

Query: 1151 ATVEKDLPGGLTPMLGDRKVEHFLGIKRKGEASNTGT 1178
            ATVEKD+PG LTPMLGDRKVEHFLGIKRK E  N+ T
Sbjct: 844  ATVEKDVPGSLTPMLGDRKVEHFLGIKRKSEPGNSDT 813

BLAST of CSPI05G31250.1 vs. TAIR 10
Match: AT3G14700.1 (SART-1 family )

HSP 1 Score: 89.4 bits (220), Expect = 2.2e-17
Identity = 79/219 (36.07%), Postives = 122/219 (55.71%), Query Frame = 0

Query: 909  EEFVWGLQLDED--AHKPEED-DVFMDDDEIPKEEYHEDVKDKDGGWTEVKDTAMEESTP 968
            ++ V  + LD++  A + E D D+    DE  + E  E  ++      ++ D A+     
Sbjct: 17   KKLVINIALDKEVLASRVEADHDLGSVRDEKERLESSERRREVCSKAEDIVDKAI----- 76

Query: 969  EENEAVAPDETIHEVPVGKGLSSALKLLKDRGTLKESIEWGGRNMDKRKSKLVGIVDEDE 1028
            + +  V  D  + E  VG GLS AL  L+++GT KE            + K+VG+     
Sbjct: 77   DNHSRVRGDGIMREADVGTGLSGALNRLREQGTFKE------------EGKVVGV----- 136

Query: 1029 PKESKSKDSRLSSLVDYKKEIHIERTDEFGRIMTPKESFRQLSHKFHGKGPGKMKQEKRM 1088
             K++  +D R     D  K+I I+R +++GRIMT KE++R L H FHGKGPGK KQEK+ 
Sbjct: 137  -KDNNHEDDRFK---DRFKDIQIQRVNKWGRIMTEKEAYRSLCHGFHGKGPGKKKQEKQR 196

Query: 1089 KQYQEELKLKQMKNADTPSLSVERMREAQAQLKTPYLVL 1125
            K++++  K KQM++++    SVER+RE  A  KTPY+VL
Sbjct: 197  KKHED--KSKQMESSER---SVERIREIHAISKTPYIVL 204