CSPI05G28360.2 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI05G28360.2
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionNuclear/nucleolar GTPase 2
LocationChr5: 26814137 .. 26819963 (-)
Sequence length1870
RNA-Seq ExpressionCSPI05G28360.2
SyntenyCSPI05G28360.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAACCATTTCTTACTCTTTTAGGTTTAGAACTTTAGATTACGCGTCACTCGTCCGCCTCTGCCTCTCCCTCTCCCTCTCCCTCGCGCAACCTCCGGCGTCTGGCGAAACCCATCTGTCTTCTCCTCCTCCGTCTCAGACCCGAGACACATCTCATATCTCAATCCCTTCGATCTCTCTTACTCTCACTTCGTCTATCTTCAACCGCCATCAACTGCCTCTCGAATTTCTTTGATTTCGCACGGTTTAAGCCAAGGTAATTTCTTATGATTTTCTTGCATATTGAACAAACCACGAGAGAAAGAAAAATGTTTATTAAACTTGTTCTGCAGTAATTTTGTGATGTGCAGGATCATATAGTTTTACCAATTATCTTTCGAATAATAAATTCACAATGATACACCTTTGCAATAGCTCTTTTTTAATAGTTTGTAGCATGTTGAGTTTGTTGAGTTGGTTTGGAAACTAAATTTGGAAACTAAATTTGGGTTGCCAACTCAAATCAGGTTTCTATTTTTTATTTTGTTTTTGTTGATTTATTCACTTAGCAAAAGGAGTAGAAGCGGATTTTTCTCTAACAAATGTTCAATTATTCTATCCAAATGCAAACATTAACTAAGGGGGAAAAACAATAAATAAAAAGAAACAAGAAAAAAACACATGTTTACTTTCCAAACAAAGGTTAGTATTTTGATGCTTAAACCTGAAGTTACCTGCCCATCCATCCAAATTCATTGTTATTCGTGTTGATATATATTTTTTTTGGATTTTATTTCATATCAATGAGCTAAAATAAATTTAAATTCAAGATGGCGAAGAAGAAGGAAAAGAAGGTAAATGTGTCTGGGAAACCAAAGCATTCTCTGGATGTTAACCGAAGCAATGAGAAAAACAAGAATGGGCGTACTGCTGCCACTGTGCGACGACTTAAGATGTATAATACGAGGCCAAAACGTGATCGGAAAGGGAAGGTGTTGAAGAATGACCTTCAGTCTAATGAGTTACCTGACACACGAATTCAACCTGATCGCCGCTGGTTTGGTATGTTGTTATTGATTTGTGTTTTATATGCTTGCAATATTTCATTTCCTACTTTTCTATTTCAATTGCTTGGTTCTTTTCTTGTTCACTATTTAATGAACCTGTATTCGAATGCTATCTTATTGTATTGTGATAAGATTTTGGAGAGGAAGTATAATGATCTGTATTTACTTGAATTGATAGGGAATACCCGAGTTGTAAACCAGAAAGAGCTTGAAATTTTCCGTGAAGAGCTTGAAAAACGAATGTCAAGTAGCTATAATGTGATTTTGAAGGAAAGGAAGCTGCCCCTTTCCCTGTTGAACGACCATCAGAAGGTAGAAGGCCTTCTCTAAAATTTGTTGATTTGAAGTCTTTTTAGGTTAACTTTCTTTTTTAGATAAGGTTTATTTATACACATATTTTAACACACCGCTGTTGCCAATGTATATTAGCTTTTGTATTGCATATATTTTTTTCAAGACTTGACAAACGGAAGGTTGTCGTATTGAGTGTGTTAGTGTTGTTATCTCTCACCTTTCCACCTGATCTTACTCACTGATTCATATAGTAGAATGAAGCTTTGAATGCTCTATGACATCATTACGTATGCTAAGACGATCATCCATTATGATATCTTGTTATAAGAGAATTATGTAGTATCTTAGGAAGACAAAAGCTTTCAATTTGGATGTGCTAATCTGGTACTTTTATTTCTGAAGCAATCCAGAGTCCATCTTCTCGACACAGAACCTTTTCAGGATGCATTTGGGCCTAAGGGGAAGAGAAAACGACCAAAGCTTTTGGCTGCGGACTATGAGTCGTTACTTCAGAAGGCTGACAAGTCCCATGGTATGAAATATTTGACTCAAATCTCTTGCATTTGCAGAAAAATTTGGGTAAACAAATGTTTTCTAGGGAGTAGTGTGATCAATTTTTGTGGTATTCCTTCAACTATTTGTTTCCTTTGAGCATTTGTTATTTCTTTTCCTTATTAAATTGAAGTTTGTACCTCAAATCTTGTAAAAAAAGTAGAAAATAAAATTGATTATTCTTTTTATGTGATTCTATTCTTTTTATTTAACGAGATATTTCAGATGATGCTGTTTTGAATTTATGTATTTCTTTGCTCCATTTTTAGTGTAAACATTTGCAATTTTGCACCAAAAAAAAAAAAAAAATCTTAAACTTGAAGATCATAGTCTCAATTATCTTGTTTGAAAATTGGAACCATGTACTTCCTCTGATAGTGTTCTCTTGTATGTCCTTGGATTATTTCATTCATCAATGAGATTGTTTATTATAAAAAAATTAAATAATTCTTTTTTAATTCAATTTTTGTTGTTGTCATCAACAATGTTAAAAATGCTGTTTTTCATATTGCCCATTCTTTATTCTTTAGATGACTTTGAGGAAAAGTATGCTGAAAATGCTACTGTAGAGGGAAGTGAAGAAGATGGTTTTAGAGACCTAGTTCGACATACTATGTTTGAGAAGGGTCAAAGTAAACGTATATGGGGTGAGCTCTACAAAGTAATCGATTCTTCAGATGTTGTTGTCCAGGTTCGTTGTAATTATTGTAACTATTATTCCTAAATGTTGTTTACCTTATTTCTAGTTTAGAAAGTAAGGTTTTCAAATAGTTTGCACAACTGCATATTATTACATTTTACATGTTAAACTTCTTATCGTTTTCCTTTCCGTGTCAGAATTGTGTTCTGTTACCCTGCAGCTTAATACAAAGCCCCCTATCATTTTTTGCATTTCCATTTGACTTCTCTTCTGATTATTTGTCATTGTTCCAGGTTCTAGATGCAAGAGATCCACAAGGAACAAGGTGTTACCATTTAGAGAGACATTTGAAAGAGCATTGCAAGCATAAACACGTGGTTCTTCTGTTAAATAAGGTTTTGCCTTATCTTTGATTTGGATTTTAAAGTTATCTACTTGGTTCTGAATTCTTTGATAACTTCTTATGGCTGACTGAGACATTTTAATAAATGGCATCTTCAGTGTGATTTGATTCCTGCTTGGGCAACAAAGGGATGGCTTAGAGTGTTATCTAAAGAATATCCAACTCTAGCGTTTCATGCAAGCATCAATAAATCCTTTGGAAAGGTATTTTTTTAATTTAATTAAGGACCGCATAATATTACCAATTTCGTCAAGATTTATTTCTTGCTAAGAGTCTAAAATGGTTATTTATGGGAAAATGTGACTCATTCTTAATCAACTTTGAAGAGATTCAGTTGCCTTTTTCTTCAACCTCTTTCAGCCCATTTTCTAACAGGGTCGTTAAATTATGATTATATGATACGGTTTCTTCTACTTTAAATTTAACATCCCTAGACTGTACATTTTTGTCCTTAGACTATAGATCGGGATATTTTAGTTGATTTTATTCAGTTTTTTTCTCCTATAAAACAACATAGTATCTGAAGCTCTCTTATGCTCCCTCTATTTGCAATGCCTTTTTTTTTTTTTAATGTTTAGAGCATATGGGTTGTGAGAAAATCCACAAGAGAATCATTTGATGTAACGTCTTTGGCAATATAGAAGAAGCATGATCTTTAACTTTGTTTTTAAAACCCTTTTTTGTTGCTCCTAGGGTTCTCTTCTATCCGTGCTGAGGCAATTTGCCCGCTTAAAAAGTGACAAGCAAGCTATCTCTGTGGGATTTGTTGGGTATCCCAATGTTGGGAAGTCATCTGTAATTAACACTCTACGGACTAAGACTGTAAGTTTTCATTCTACTTTGAAATGTTTTGTGTTAATGTGAAAAAAATTTTAGTCTATTTTTTTAGAACATTCATTTGGTGGTATGTCTAAATTGAATTTTGAATGAAAAACTTGCACTAAAAAATTAGCGTACAAGAGATTCTATCTTGTCTATCTTGTTAAGCTTCCGAAACAATTATGAAAAAATTTGGATGTTCTAAATTTCATTACCCGGATCTTCTGTAACTGTGAGGACTTTTGTTAGGTGTAACATGTTTTCTAGGGAATATTCTTATTCATTTGAATGTTTATGAGCATGTGGCTTGCTTATTGAATATTATCTTTTGATATATTCCCGTACCAATGAATCACAAGTGTGATAAGCCACCGATTTGATATGACGCTACGAGAAATGCAGAATCTACCAGTAGAGTTGGAGGGAAAATTCTAGGAAATAATAGTTGGAAGGGGGTGGAGGTAACAAGTCTGACACACAGTTTGTTTGTTAAGCCTAGTTTCGAGTAGCAGGGTAGCTCACTGTTGCCTGATCTAAAAAAAATTGGAAATGTTAGAGGTGAAGGTAACAGGGCTGGCCTAGTTTTTTTGTTAAGCTTAGCGTCAAGTAGCAGGGTAGCCAATTGTTGACAGGGCTAAAAATAATTGGCAAACCCAGAGGAGAAGGTAAAAAGTCCGATACCCAGTTTGTTGAAGTCTAGTTTCAACTATCAGGGCAGCCAATTGTTGACTGGTCTAAAAATAATTGGAAAACCTAGAGGCATGCGAATAATAGATTGAAGATTGAAAGGTGGCTGAATCTTTAAATTTTTTCTTGGACTTTTCTTTCGTATCGATGGAAATGCTTGGAACAATCTGGAGTTCAGATGTGCTCATGTGCAAGAAAGTGTTCTTGATCTGTGTGTAAAATATATATTTTTAAAGGTTTTCTAAAAGCTGGAAACTTTTGGTTTTTAAGATAAAAGAGATTACGAGCATATGGTACATTCTGGTATTCAAAAAAAAAAAAAGAAAAAACACTTTAATCTGAATATATTGCATGAAGCTTGTAAGTTGAACGTCCAACAAAGCACTCCCATTAATTTTTTCACTGCTAAGAAAGTATGTCATAGAAGCCATGGTAACTTTTTAGGGGTCTGTTAACTATCACTTAAAAGATTGTTTCAACCTCTCGATCTCTGTTGCTTGTTGAAGTGATACTATAGTGAATCCCTTATACATGCATACATGATACATGCATGTTATACATACATATACATGAAATGTAACTTTGCCTTTCTTTTATTAGGTATGCAAAGTTGCACCTATTCCAGGAGAAACTAAAGTTTGGCAGTATATAACTCTTACAAAGAGGATTTTCTTGATTGACTGCCCGGGAGTCGTTTATCAAAATAGTGACACTGAAACTGATATTGTCCTTAAGGGCGTGGTATGTTTTTTCTCATTTTCATTTGCATTGCCTTTCTGGTTTCTACAGTAGCCATACAGATATTTTCAATTAATTGGTATGGCAATTATTAACCTTTTTGCCAACTTGTTAATATTTTATATTACTGTCTGTCTGTATAATTTGGTATTTGGGACTTTGTACTAGGTACGAGTTACAAATTTGGAGGATGCATCGGAACATATTGGAGAAGTATTGAAGCGTGTGAAGAAGGAACACCTTGAAAGAGCGTACAAGATAAAAAACTGGTGCATGACTATCCTTTTTGCATGATTTTGCAACCGTTATTATTTGGTTCAAATTTAATTTTCAATCATTTTTTAATTTCCAAAATGCATTTTTAGTCACTTTTAAAGAAGTAAAATTCCATTTTGGTTCTTACTGTTATTATCTAATACCCACCTCAACTACATGTCCACGTTAAGTGAGGATCAAAACCATTATTTTCATCAAGTGTAGGGACTGAATAGAAAATAGCAAATTTGACAATTTAGAGGGAAAGAAGAAATAAGCCTAAATGTTTAGGGACTAAAAGAGTGTAAAGCTTAAAAGGTTGTGGGAGGAAAATAAAATTTAAAAGTATCATCATCTTGGTTGATAGAAAACTTGATGGAGTTGCAGCAGCC

mRNA sequence

AAAAAACCATTTCTTACTCTTTTAGGTTTAGAACTTTAGATTACGCGTCACTCGTCCGCCTCTGCCTCTCCCTCTCCCTCTCCCTCGCGCAACCTCCGGCGTCTGGCGAAACCCATCTGTCTTCTCCTCCTCCGTCTCAGACCCGAGACACATCTCATATCTCAATCCCTTCGATCTCTCTTACTCTCACTTCGTCTATCTTCAACCGCCATCAACTGCCTCTCGAATTTCTTTGATTTCGCACGGTTTAAGCCAAGATGGCGAAGAAGAAGGAAAAGAAGGTAAATGTGTCTGGGAAACCAAAGCATTCTCTGGATGTTAACCGAAGCAATGAGAAAAACAAGAATGGGCGTACTGCTGCCACTGTGCGACGACTTAAGATGTATAATACGAGGCCAAAACGTGATCGGAAAGGGAAGGTGTTGAAGAATGACCTTCAGTCTAATGAGTTACCTGACACACGAATTCAACCTGATCGCCGCTGGTTTGGGAATACCCGAGTTGTAAACCAGAAAGAGCTTGAAATTTTCCGTGAAGAGCTTGAAAAACGAATGTCAAGTAGCTATAATGTGATTTTGAAGGAAAGGAAGCTGCCCCTTTCCCTGTTGAACGACCATCAGAAGCAATCCAGAGTCCATCTTCTCGACACAGAACCTTTTCAGGATGCATTTGGGCCTAAGGGGAAGAGAAAACGACCAAAGCTTTTGGCTGCGGACTATGAGTCGTTACTTCAGAAGGCTGACAAGTCCCATGATGACTTTGAGGAAAAGTATGCTGAAAATGCTACTGTAGAGGGAAGTGAAGAAGATGGTTTTAGAGACCTAGTTCGACATACTATGTTTGAGAAGGGTCAAAGTAAACGTATATGGGGTGAGCTCTACAAAGTAATCGATTCTTCAGATGTTGTTGTCCAGGTTCTAGATGCAAGAGATCCACAAGGAACAAGGTGTTACCATTTAGAGAGACATTTGAAAGAGCATTGCAAGCATAAACACGTGGTTCTTCTGTTAAATAAGTGTGATTTGATTCCTGCTTGGGCAACAAAGGGATGGCTTAGAGTGTTATCTAAAGAATATCCAACTCTAGCGTTTCATGCAAGCATCAATAAATCCTTTGGAAAGGGTTCTCTTCTATCCGTGCTGAGGCAATTTGCCCGCTTAAAAAGTGACAAGCAAGCTATCTCTGTGGGATTTGTTGGGTATCCCAATGTTGGGAAGTCATCTGTAATTAACACTCTACGGACTAAGACTGTATGCAAAGTTGCACCTATTCCAGGAGAAACTAAAGTTTGGCAGTATATAACTCTTACAAAGAGGATTTTCTTGATTGACTGCCCGGGAGTCGTTTATCAAAATAGTGACACTGAAACTGATATTGTCCTTAAGGGCGTGGTACGAGTTACAAATTTGGAGGATGCATCGGAACATATTGGAGAAGTATTGAAGCGTGTGAAGAAGGAACACCTTGAAAGAGCGTACAAGATAAAAAACTGGTGCATGACTATCCTTTTTGCATGATTTTGCAACCGTTATTATTTGGTTCAAATTTAATTTTCAATCATTTTTTAATTTCCAAAATGCATTTTTAGTCACTTTTAAAGAAGTAAAATTCCATTTTGGTTCTTACTGTTATTATCTAATACCCACCTCAACTACATGTCCACGTTAAGTGAGGATCAAAACCATTATTTTCATCAAGTGTAGGGACTGAATAGAAAATAGCAAATTTGACAATTTAGAGGGAAAGAAGAAATAAGCCTAAATGTTTAGGGACTAAAAGAGTGTAAAGCTTAAAAGGTTGTGGGAGGAAAATAAAATTTAAAAGTATCATCATCTTGGTTGATAGAAAACTTGATGGAGTTGCAGCAGCC

Coding sequence (CDS)

ATGGCGAAGAAGAAGGAAAAGAAGGTAAATGTGTCTGGGAAACCAAAGCATTCTCTGGATGTTAACCGAAGCAATGAGAAAAACAAGAATGGGCGTACTGCTGCCACTGTGCGACGACTTAAGATGTATAATACGAGGCCAAAACGTGATCGGAAAGGGAAGGTGTTGAAGAATGACCTTCAGTCTAATGAGTTACCTGACACACGAATTCAACCTGATCGCCGCTGGTTTGGGAATACCCGAGTTGTAAACCAGAAAGAGCTTGAAATTTTCCGTGAAGAGCTTGAAAAACGAATGTCAAGTAGCTATAATGTGATTTTGAAGGAAAGGAAGCTGCCCCTTTCCCTGTTGAACGACCATCAGAAGCAATCCAGAGTCCATCTTCTCGACACAGAACCTTTTCAGGATGCATTTGGGCCTAAGGGGAAGAGAAAACGACCAAAGCTTTTGGCTGCGGACTATGAGTCGTTACTTCAGAAGGCTGACAAGTCCCATGATGACTTTGAGGAAAAGTATGCTGAAAATGCTACTGTAGAGGGAAGTGAAGAAGATGGTTTTAGAGACCTAGTTCGACATACTATGTTTGAGAAGGGTCAAAGTAAACGTATATGGGGTGAGCTCTACAAAGTAATCGATTCTTCAGATGTTGTTGTCCAGGTTCTAGATGCAAGAGATCCACAAGGAACAAGGTGTTACCATTTAGAGAGACATTTGAAAGAGCATTGCAAGCATAAACACGTGGTTCTTCTGTTAAATAAGTGTGATTTGATTCCTGCTTGGGCAACAAAGGGATGGCTTAGAGTGTTATCTAAAGAATATCCAACTCTAGCGTTTCATGCAAGCATCAATAAATCCTTTGGAAAGGGTTCTCTTCTATCCGTGCTGAGGCAATTTGCCCGCTTAAAAAGTGACAAGCAAGCTATCTCTGTGGGATTTGTTGGGTATCCCAATGTTGGGAAGTCATCTGTAATTAACACTCTACGGACTAAGACTGTATGCAAAGTTGCACCTATTCCAGGAGAAACTAAAGTTTGGCAGTATATAACTCTTACAAAGAGGATTTTCTTGATTGACTGCCCGGGAGTCGTTTATCAAAATAGTGACACTGAAACTGATATTGTCCTTAAGGGCGTGGTACGAGTTACAAATTTGGAGGATGCATCGGAACATATTGGAGAAGTATTGAAGCGTGTGAAGAAGGAACACCTTGAAAGAGCGTACAAGATAAAAAACTGGTGCATGACTATCCTTTTTGCATGA

Protein sequence

MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDLQSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDHQKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEGSEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNWCMTILFA*
Homology
BLAST of CSPI05G28360.2 vs. ExPASy Swiss-Prot
Match: A2XGQ1 (Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. indica OX=39946 GN=NUG2 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 3.7e-198
Identity = 346/421 (82.19%), Postives = 380/421 (90.26%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEK---------NKNGRTAATVRRLKMYNTRPKRDR 60
           MAKKKE+ VNVSGKP+HSLDVNR+N+K            GR+AATVRRLKMY  RP RDR
Sbjct: 1   MAKKKERAVNVSGKPRHSLDVNRANDKKGAGGGAGGGGGGRSAATVRRLKMYKMRPLRDR 60

Query: 61  KGKVLKNDLQSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERK 120
            GK+LK+DLQS ELP+TRI+PDRRWFGNTRVVNQKELE FREEL+ R+S++YNVILKERK
Sbjct: 61  GGKILKHDLQSKELPNTRIEPDRRWFGNTRVVNQKELEFFREELQSRLSNNYNVILKERK 120

Query: 121 LPLSLLNDHQKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEK 180
           LPLSLL DHQKQ+R HLLDTEPF+ AFGPKGKRKRPKL+A DYESLL+KAD S   FE+K
Sbjct: 121 LPLSLLQDHQKQARAHLLDTEPFEHAFGPKGKRKRPKLMALDYESLLKKADDSQGAFEDK 180

Query: 181 YAENATVEGSEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRC 240
           +A    ++  EEDG RDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDP GTRC
Sbjct: 181 HATAKLLKEEEEDGLRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRC 240

Query: 241 YHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSL 300
           YHLE+HLKE+ KHKH+V LLNKCDL+PAWATKGWLR LSK+YPTLAFHASIN SFGKGSL
Sbjct: 241 YHLEKHLKENAKHKHLVFLLNKCDLVPAWATKGWLRTLSKDYPTLAFHASINSSFGKGSL 300

Query: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLT 360
           LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLR+K+VCKVAPIPGETKVWQYITLT
Sbjct: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRSKSVCKVAPIPGETKVWQYITLT 360

Query: 361 KRIFLIDCPGVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKN 413
           KRIFLIDCPGVVYQN+D+ETDIVLKGVVRVTNL DASEHIGEVL+RVKKEHL+RAYKI++
Sbjct: 361 KRIFLIDCPGVVYQNNDSETDIVLKGVVRVTNLADASEHIGEVLRRVKKEHLKRAYKIED 420

BLAST of CSPI05G28360.2 vs. ExPASy Swiss-Prot
Match: Q10LF7 (Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NUG2 PE=1 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 3.7e-198
Identity = 346/421 (82.19%), Postives = 380/421 (90.26%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEK---------NKNGRTAATVRRLKMYNTRPKRDR 60
           MAKKKE+ VNVSGKP+HSLDVNR+N+K            GR+AATVRRLKMY  RP RDR
Sbjct: 1   MAKKKERAVNVSGKPRHSLDVNRANDKKGAGGGAGGGGGGRSAATVRRLKMYKMRPLRDR 60

Query: 61  KGKVLKNDLQSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERK 120
            GK+LK+DLQS ELP+TRI+PDRRWFGNTRVVNQKELE FREEL+ R+S++YNVILKERK
Sbjct: 61  GGKILKHDLQSKELPNTRIEPDRRWFGNTRVVNQKELEFFREELQSRLSNNYNVILKERK 120

Query: 121 LPLSLLNDHQKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEK 180
           LPLSLL DHQKQ+R HLLDTEPF+ AFGPKGKRKRPKL+A DYESLL+KAD S   FE+K
Sbjct: 121 LPLSLLQDHQKQARAHLLDTEPFEHAFGPKGKRKRPKLMALDYESLLKKADDSQGAFEDK 180

Query: 181 YAENATVEGSEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRC 240
           +A    ++  EEDG RDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDP GTRC
Sbjct: 181 HATAKLLKEEEEDGLRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRC 240

Query: 241 YHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSL 300
           YHLE+HLKE+ KHKH+V LLNKCDL+PAWATKGWLR LSK+YPTLAFHASIN SFGKGSL
Sbjct: 241 YHLEKHLKENAKHKHLVFLLNKCDLVPAWATKGWLRTLSKDYPTLAFHASINSSFGKGSL 300

Query: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLT 360
           LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLR+K+VCKVAPIPGETKVWQYITLT
Sbjct: 301 LSVLRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRSKSVCKVAPIPGETKVWQYITLT 360

Query: 361 KRIFLIDCPGVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKN 413
           KRIFLIDCPGVVYQN+D+ETDIVLKGVVRVTNL DASEHIGEVL+RVKKEHL+RAYKI++
Sbjct: 361 KRIFLIDCPGVVYQNNDSETDIVLKGVVRVTNLADASEHIGEVLRRVKKEHLKRAYKIED 420

BLAST of CSPI05G28360.2 vs. ExPASy Swiss-Prot
Match: Q9C923 (Nuclear/nucleolar GTPase 2 OS=Arabidopsis thaliana OX=3702 GN=NUG2 PE=1 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 4.6e-193
Identity = 340/412 (82.52%), Postives = 374/412 (90.78%), Query Frame = 0

Query: 4   KKEKKVNVSGKPKHSLDVNRSNEKNK--NGRTAATVRRLKMYNTRPKRDRKGKVLKNDLQ 63
           KKEKK NVSGKPKHSLD NR++ K K    R+ +TV RLKMY TRPKR+  GK+L N+ Q
Sbjct: 3   KKEKKANVSGKPKHSLDANRADGKKKTTETRSKSTVNRLKMYKTRPKRNAGGKILSNEYQ 62

Query: 64  SNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDHQ 123
           S ELP++RI PDRRWFGNTRVVNQKELE FREEL+ +MSS+YNVILKERKLP+SLL D++
Sbjct: 63  SKELPNSRIAPDRRWFGNTRVVNQKELEYFREELQTKMSSNYNVILKERKLPMSLLTDNK 122

Query: 124 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVE-G 183
           KQSRVHLLD EPFQDAFG K KRKRPKL+A+DYE+L++KA +S D FEEK     + E G
Sbjct: 123 KQSRVHLLDMEPFQDAFGRKTKRKRPKLVASDYEALVKKAAESQDAFEEKNGAGPSGEGG 182

Query: 184 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 243
            EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDV+VQV+DARDPQGTRC+HLE+ LKE
Sbjct: 183 EEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVIVQVIDARDPQGTRCHHLEKTLKE 242

Query: 244 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 303
           H KHKH++LLLNKCDL+PAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 243 HHKHKHMILLLNKCDLVPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 302

Query: 304 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 363
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 303 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 362

Query: 364 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQ+ DTETDIVLKGVVRVTNLEDASEHIGEVL+RVKKEHL+RAYKIK+W
Sbjct: 363 GVVYQSRDTETDIVLKGVVRVTNLEDASEHIGEVLRRVKKEHLQRAYKIKDW 414

BLAST of CSPI05G28360.2 vs. ExPASy Swiss-Prot
Match: Q99LH1 (Nucleolar GTP-binding protein 2 OS=Mus musculus OX=10090 GN=Gnl2 PE=1 SV=2)

HSP 1 Score: 434.1 bits (1115), Expect = 1.8e-120
Identity = 222/418 (53.11%), Postives = 300/418 (71.77%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNR-SNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKN- 60
           M K K K  +   +   S + +R      +N R   T+RRL MY  + +R+ +GKV+K  
Sbjct: 1   MVKPKYKGRSTINRSAASTNPDRVQGAGGQNMRDRGTIRRLNMYRQKERRNSRGKVIKPL 60

Query: 61  DLQSNELPDT--RIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSL 120
             QS     T  R++P+ +WFGNTRV+ Q  L+ F+EE++K M   Y V++K+ KLP+SL
Sbjct: 61  QYQSTVASGTVARVEPNIKWFGNTRVIKQASLQKFQEEMDKVMKDPYKVVMKQSKLPMSL 120

Query: 121 LND--HQKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAE 180
           L+D      ++VH+LDTE F+  FGPK +RKRP L A+D +SLL+ A+ S + +++    
Sbjct: 121 LHDRIQPHNAKVHILDTESFESTFGPKSQRKRPNLFASDMQSLLENAEMSTESYDQGKDR 180

Query: 181 NATVEGSEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHL 240
           +  +   E+ G R+  +  +++KGQSKRIWGELYKVIDSSDVVVQVLDARDP GTR  H+
Sbjct: 181 DLVM---EDTGVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSPHI 240

Query: 241 ERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSV 300
           E +LK+    KH++ +LNKCDL+P WATK W+ VLS++YPTLAFHAS+   FGKG+ + +
Sbjct: 241 EAYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQL 300

Query: 301 LRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRI 360
           LRQF +L +DK+ ISVGF+GYPNVGKSSVINTLR+K VC VAPI GETKVWQYITL +RI
Sbjct: 301 LRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRI 360

Query: 361 FLIDCPGVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           FLIDCPGVVY + D+ETDIVLKGVV+V  ++   +HIG VL+R K E++ + YKI++W
Sbjct: 361 FLIDCPGVVYPSEDSETDIVLKGVVQVEKIKAPQDHIGAVLERAKPEYISKTYKIESW 415

BLAST of CSPI05G28360.2 vs. ExPASy Swiss-Prot
Match: Q13823 (Nucleolar GTP-binding protein 2 OS=Homo sapiens OX=9606 GN=GNL2 PE=1 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.8e-120
Identity = 221/418 (52.87%), Postives = 299/418 (71.53%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNR-SNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKN- 60
           M K K K  +     K S + +R      +N R  AT+RRL MY  + +R+ +GK++K  
Sbjct: 1   MVKPKYKGRSTINPSKASTNPDRVQGAGGQNMRDRATIRRLNMYRQKERRNSRGKIIKPL 60

Query: 61  DLQSNELPDT--RIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSL 120
             QS     T  R++P+ +WFGNTRV+ Q  L+ F+EE++  M   Y V++K+ KLP+SL
Sbjct: 61  QYQSTVASGTVARVEPNIKWFGNTRVIKQSSLQKFQEEMDTVMKDPYKVVMKQSKLPMSL 120

Query: 121 LNDHQK--QSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAE 180
           L+D  +    +VH+LDTE F+  FGPK +RKRP L A+D +SL++ A+ S + +++    
Sbjct: 121 LHDRIRPHNLKVHILDTESFETTFGPKSQRKRPNLFASDMQSLIENAEMSTESYDQGKDR 180

Query: 181 NATVEGSEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHL 240
           +     +E+ G R+  +  +++KGQSKRIWGELYKVIDSSDVVVQVLDARDP GTR  H+
Sbjct: 181 DLV---TEDTGVRNEAQEEIYKKGQSKRIWGELYKVIDSSDVVVQVLDARDPMGTRSPHI 240

Query: 241 ERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSV 300
           E +LK+    KH++ +LNKCDL+P WATK W+ VLS++YPTLAFHAS+   FGKG+ + +
Sbjct: 241 ETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLAFHASLTNPFGKGAFIQL 300

Query: 301 LRQFARLKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRI 360
           LRQF +L +DK+ ISVGF+GYPNVGKSSVINTLR+K VC VAPI GETKVWQYITL +RI
Sbjct: 301 LRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQYITLMRRI 360

Query: 361 FLIDCPGVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           FLIDCPGVVY + D+ETDIVLKGVV+V  ++   +HIG VL+R K E++ + YKI +W
Sbjct: 361 FLIDCPGVVYPSEDSETDIVLKGVVQVEKIKSPEDHIGAVLERAKPEYISKTYKIDSW 415

BLAST of CSPI05G28360.2 vs. ExPASy TrEMBL
Match: A0A0A0KTZ3 (Nuclear/nucleolar GTPase 2 OS=Cucumis sativus OX=3659 GN=Csa_5G631500 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 7.3e-234
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 412

BLAST of CSPI05G28360.2 vs. ExPASy TrEMBL
Match: A0A5D3BS64 (Nuclear/nucleolar GTPase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00410 PE=3 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 8.1e-233
Identity = 410/412 (99.51%), Postives = 411/412 (99.76%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGR+AATVRRLKMYNTRPKRDRKGKVLKNDL
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRSAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 412

BLAST of CSPI05G28360.2 vs. ExPASy TrEMBL
Match: A0A6J1K2N0 (Nuclear/nucleolar GTPase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489529 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.2e-221
Identity = 390/412 (94.66%), Postives = 401/412 (97.33%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           M KK E+KV+VSGKPKHSLD NRS + NKN R+AATVRRLKMYNTRPKRDRKGKVLK++L
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRS-DGNKNARSAATVRRLKMYNTRPKRDRKGKVLKHEL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMSSSYNVILKE+KLPLSLLNDH
Sbjct: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLL+KADKSHDDF+EKYAENAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETD+VLKGVVRVTNLED SEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLERAYKIKNW 411

BLAST of CSPI05G28360.2 vs. ExPASy TrEMBL
Match: A0A6J1FGU6 (Nuclear/nucleolar GTPase 2 OS=Cucurbita moschata OX=3662 GN=LOC111445557 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.1e-220
Identity = 388/412 (94.17%), Postives = 400/412 (97.09%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           M KK E+KV+VSGKPKHSLD NRS + NKN R+AATVRRLKMYNTRP+RDRKGKVLK++L
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRS-DGNKNARSAATVRRLKMYNTRPRRDRKGKVLKHEL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMSSSYNVILKE+KLPLSLLNDH
Sbjct: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLL+KADKSHDDF+EKYAENAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
            EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 GEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETD+VLKGVVRVTNLED SEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLERAYKIKNW 411

BLAST of CSPI05G28360.2 vs. ExPASy TrEMBL
Match: A0A6J1C0B4 (Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 3.9e-219
Identity = 386/412 (93.69%), Postives = 399/412 (96.84%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           M KKKEKKVNVSGKPKHSLDVNRS + NKN R+AATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MTKKKEKKVNVSGKPKHSLDVNRS-DANKNSRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMS SYNVILKE+KLPLSLLNDH
Sbjct: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSCSYNVILKEKKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPF+DAFGPKGKRKRPKLLAADYESLL+KADKSHD FEE +A+NAT EG
Sbjct: 121 QKQSRVHLLDTEPFEDAFGPKGKRKRPKLLAADYESLLKKADKSHDAFEENHADNATAEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SE DGFRDLVRH MFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEGDGFRDLVRHNMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSD+ETDIVLKGVVRVTNLEDASEHIGEVLKRVK+EHLERAYKIK+W
Sbjct: 361 GVVYQNSDSETDIVLKGVVRVTNLEDASEHIGEVLKRVKREHLERAYKIKDW 411

BLAST of CSPI05G28360.2 vs. NCBI nr
Match: XP_004142347.1 (nuclear/nucleolar GTPase 2 [Cucumis sativus] >KGN52399.1 hypothetical protein Csa_008867 [Cucumis sativus])

HSP 1 Score: 819.3 bits (2115), Expect = 1.5e-233
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 412

BLAST of CSPI05G28360.2 vs. NCBI nr
Match: TYK01950.1 (nuclear/nucleolar GTPase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 815.8 bits (2106), Expect = 1.7e-232
Identity = 410/412 (99.51%), Postives = 411/412 (99.76%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGR+AATVRRLKMYNTRPKRDRKGKVLKNDL
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRSAATVRRLKMYNTRPKRDRKGKVLKNDL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH
Sbjct: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 412

BLAST of CSPI05G28360.2 vs. NCBI nr
Match: XP_038890331.1 (nuclear/nucleolar GTPase 2 [Benincasa hispida] >XP_038890332.1 nuclear/nucleolar GTPase 2 [Benincasa hispida])

HSP 1 Score: 803.1 bits (2073), Expect = 1.1e-228
Identity = 405/412 (98.30%), Postives = 408/412 (99.03%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           MAKKKEKKVNVSGKPKHSLDVNRSNE NKNGR+AATVRRLKMYNTRPKRDRKGKVLKN+L
Sbjct: 1   MAKKKEKKVNVSGKPKHSLDVNRSNE-NKNGRSAATVRRLKMYNTRPKRDRKGKVLKNEL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH
Sbjct: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENAT+EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATIEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIK W
Sbjct: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKEW 411

BLAST of CSPI05G28360.2 vs. NCBI nr
Match: XP_023550727.1 (nuclear/nucleolar GTPase 2 [Cucurbita pepo subsp. pepo] >XP_023550729.1 nuclear/nucleolar GTPase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 777.3 bits (2006), Expect = 6.6e-221
Identity = 390/412 (94.66%), Postives = 401/412 (97.33%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           M KK E+KV+VSGKPKHSLD NRS + NKN R+AATVRRLKMYNTRPKRDRKGKVLK++L
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRS-DGNKNARSAATVRRLKMYNTRPKRDRKGKVLKHEL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMSSSYNVILKE+KLPLSLLNDH
Sbjct: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLL+KADKSHDDF+EKYAENAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETD+VLKGVVRVTNLED SEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLERAYKIKNW 411

BLAST of CSPI05G28360.2 vs. NCBI nr
Match: XP_022993572.1 (nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993573.1 nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993574.1 nuclear/nucleolar GTPase 2 [Cucurbita maxima])

HSP 1 Score: 777.3 bits (2006), Expect = 6.6e-221
Identity = 390/412 (94.66%), Postives = 401/412 (97.33%), Query Frame = 0

Query: 1   MAKKKEKKVNVSGKPKHSLDVNRSNEKNKNGRTAATVRRLKMYNTRPKRDRKGKVLKNDL 60
           M KK E+KV+VSGKPKHSLD NRS + NKN R+AATVRRLKMYNTRPKRDRKGKVLK++L
Sbjct: 1   MTKKTERKVSVSGKPKHSLDANRS-DGNKNARSAATVRRLKMYNTRPKRDRKGKVLKHEL 60

Query: 61  QSNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDH 120
           QS ELPDTRIQPDRRWFGNTRVVNQKELE FREEL+KRMSSSYNVILKE+KLPLSLLNDH
Sbjct: 61  QSKELPDTRIQPDRRWFGNTRVVNQKELEFFREELQKRMSSSYNVILKEKKLPLSLLNDH 120

Query: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVEG 180
           QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLL+KADKSHDDF+EKYAENAT EG
Sbjct: 121 QKQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLKKADKSHDDFDEKYAENATNEG 180

Query: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240
           SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE
Sbjct: 181 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 240

Query: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300
           HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR
Sbjct: 241 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 300

Query: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 360
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 301 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 360

Query: 361 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQNSDTETD+VLKGVVRVTNLED SEHIGEVLKRVKKEHLERAYKIKNW
Sbjct: 361 GVVYQNSDTETDVVLKGVVRVTNLEDPSEHIGEVLKRVKKEHLERAYKIKNW 411

BLAST of CSPI05G28360.2 vs. TAIR 10
Match: AT1G52980.1 (GTP-binding family protein )

HSP 1 Score: 675.2 bits (1741), Expect = 3.3e-194
Identity = 340/412 (82.52%), Postives = 374/412 (90.78%), Query Frame = 0

Query: 4   KKEKKVNVSGKPKHSLDVNRSNEKNK--NGRTAATVRRLKMYNTRPKRDRKGKVLKNDLQ 63
           KKEKK NVSGKPKHSLD NR++ K K    R+ +TV RLKMY TRPKR+  GK+L N+ Q
Sbjct: 3   KKEKKANVSGKPKHSLDANRADGKKKTTETRSKSTVNRLKMYKTRPKRNAGGKILSNEYQ 62

Query: 64  SNELPDTRIQPDRRWFGNTRVVNQKELEIFREELEKRMSSSYNVILKERKLPLSLLNDHQ 123
           S ELP++RI PDRRWFGNTRVVNQKELE FREEL+ +MSS+YNVILKERKLP+SLL D++
Sbjct: 63  SKELPNSRIAPDRRWFGNTRVVNQKELEYFREELQTKMSSNYNVILKERKLPMSLLTDNK 122

Query: 124 KQSRVHLLDTEPFQDAFGPKGKRKRPKLLAADYESLLQKADKSHDDFEEKYAENATVE-G 183
           KQSRVHLLD EPFQDAFG K KRKRPKL+A+DYE+L++KA +S D FEEK     + E G
Sbjct: 123 KQSRVHLLDMEPFQDAFGRKTKRKRPKLVASDYEALVKKAAESQDAFEEKNGAGPSGEGG 182

Query: 184 SEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKE 243
            EEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDV+VQV+DARDPQGTRC+HLE+ LKE
Sbjct: 183 EEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVIVQVIDARDPQGTRCHHLEKTLKE 242

Query: 244 HCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFAR 303
           H KHKH++LLLNKCDL+PAWATKGWLRVLSKEYPTLAFHAS+NKSFGKGSLLSVLRQFAR
Sbjct: 243 HHKHKHMILLLNKCDLVPAWATKGWLRVLSKEYPTLAFHASVNKSFGKGSLLSVLRQFAR 302

Query: 304 LKSDKQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCP 363
           LKSDKQAISVGFVGYPNVGKSSVINTLRTK VCKVAPIPGETKVWQYITLTKRIFLIDCP
Sbjct: 303 LKSDKQAISVGFVGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCP 362

Query: 364 GVVYQNSDTETDIVLKGVVRVTNLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
           GVVYQ+ DTETDIVLKGVVRVTNLEDASEHIGEVL+RVKKEHL+RAYKIK+W
Sbjct: 363 GVVYQSRDTETDIVLKGVVRVTNLEDASEHIGEVLRRVKKEHLQRAYKIKDW 414

BLAST of CSPI05G28360.2 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 162.5 bits (410), Expect = 7.2e-40
Identity = 96/270 (35.56%), Postives = 144/270 (53.33%), Query Frame = 0

Query: 167 DFEEKYAENATVEGSEEDGFRDLVRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDP 226
           D E+   E  T+E        DL +         +  + EL KVI+ SDV+++VLDARDP
Sbjct: 98  DDEDTKTEGETIE--------DLPKVVNVRDNSERAFYKELVKVIELSDVILEVLDARDP 157

Query: 227 QGTRCYHLERHLKEHCKHKHVVLLLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKS- 286
            GTRC  +ER + +   +KH+VLLLNK DL+P  A + WL  L +E+P +AF  S  +  
Sbjct: 158 LGTRCTDMERMVMQAGPNKHLVLLLNKIDLVPREAAEKWLMYLREEFPAVAFKCSTQEQR 217

Query: 287 ----------------------FGKGSLLSVLRQFARLKSDKQAISVGFVGYPNVGKSSV 346
                                  G  +L+ +L+ ++R    K++I+VG +G PNVGKSS+
Sbjct: 218 SNLGWKSSKASKPSNMLQTSDCLGADTLIKLLKNYSRSHELKKSITVGIIGLPNVGKSSL 277

Query: 347 INTLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCPGVV-YQNSDTETDIVLKGVVRVT 406
           IN+L+   V  V   PG T+  Q + L K + L+DCPGVV  ++S  +  I L+   R+ 
Sbjct: 278 INSLKRAHVVNVGATPGLTRSLQEVHLDKNVKLLDCPGVVMLKSSGNDASIALRNCKRIE 337

Query: 407 NLEDASEHIGEVLKRVKKEHLERAYKIKNW 413
            L+D    + E+LK   K+ L   YKI ++
Sbjct: 338 KLDDPVSPVKEILKLCPKDMLVTLYKIPSF 359

BLAST of CSPI05G28360.2 vs. TAIR 10
Match: AT2G27200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 4.1e-27
Identity = 78/261 (29.89%), Postives = 129/261 (49.43%), Query Frame = 0

Query: 193 TMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLN 252
           T FEK     IW +L++V++ SD++V V+DARDP   RC  LE + +E  +HK  +LL+N
Sbjct: 149 TPFEKNLD--IWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKTMLLVN 208

Query: 253 KCDLIPAWATKGWLRVLSKEYPTLAFHAS----------------------------INK 312
           K DL+P++  + W    S+      F ++                              K
Sbjct: 209 KADLLPSYVREKWAEYFSRNNILFVFWSAKAATATLEGKPLKEQWRAPDTTQKTDNPAVK 268

Query: 313 SFGKGSLLSVLR----QFARLKSDK-----------QAISVGFVGYPNVGKSSVINTLRT 372
            +G+  LL  L+    +  +++  +           + + VGFVGYPNVGKSS IN L  
Sbjct: 269 VYGRDDLLDRLKLEALEIVKMRKSRGVSATSTESHCEQVVVGFVGYPNVGKSSTINALVG 328

Query: 373 KTVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQN-SDTETDIVLKGVVRVTNLEDAS 410
           +    V   PG+TK +Q + +++ + L DCPG+V+ + S +  ++V  GV+ +  + +  
Sbjct: 329 QKRTGVTSTPGKTKHFQTLIISEDLMLCDCPGLVFPSFSSSRYEMVASGVLPIDRMTEHL 388

BLAST of CSPI05G28360.2 vs. TAIR 10
Match: AT1G08410.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 7.7e-26
Identity = 78/265 (29.43%), Postives = 127/265 (47.92%), Query Frame = 0

Query: 193 TMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVLLLN 252
           T FEK     IW +L++V++ SD++V V+DARDP   RC  LE + +E  +HK ++LL+N
Sbjct: 149 TPFEKNLD--IWRQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKIMLLVN 208

Query: 253 KCDLIPAWATKGWLRVLSKEYPTLAFHASINKS--------------------------- 312
           K DL+P    + W            F ++I  +                           
Sbjct: 209 KADLLPTDVREKWAEYFRLNNILFVFWSAIAATATLEGKVLKEQWRQPDNLQKTDDPDIM 268

Query: 313 -FGKGSLLSVL----RQFARLKSDKQA---------------ISVGFVGYPNVGKSSVIN 372
            +G+  LLS L    ++  ++++ + A                 VGFVGYPNVGKSS IN
Sbjct: 269 IYGRDELLSRLQFEAQEIVKVRNSRAASVSSQSWTGEYQRDQAVVGFVGYPNVGKSSTIN 328

Query: 373 TLRTKTVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQN-SDTETDIVLKGVVRVTNL 410
            L  +    V   PG+TK +Q + ++  + L DCPG+V+ + S +  +++  GV+ +  +
Sbjct: 329 ALVGQKRTGVTSTPGKTKHFQTLIISDELMLCDCPGLVFPSFSSSRYEMIASGVLPIDRM 388

BLAST of CSPI05G28360.2 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.8e-14
Identity = 55/192 (28.65%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 190 VRHTMFEKGQSKRIWGELYKVIDSSDVVVQVLDARDPQGTRCYHLERHLKEHCKHKHVVL 249
           +R   +  G   +   EL + +   DVV++V DAR P  T    ++  L     ++  +L
Sbjct: 93  LRPVQWYPGHIMKTEKELREQLKLMDVVIEVRDARIPLSTTHPKMDAWL----GNRKRIL 152

Query: 250 LLNKCDLIPAWATKGWLRVLSKEYPTLAFHASINKSFGKGSLLSVLRQFARLKSD----- 309
           +LN+ D+I       W R  +K+   + F    N   G G+ + + R    L  D     
Sbjct: 153 VLNREDMISNDDRNDWARYFAKQGIKVIF---TNGKLGMGA-MKLGRLAKSLAGDVNGKR 212

Query: 310 ------KQAISVGFVGYPNVGKSSVINTLRTKTVCKVAPIPGETKVWQYITLTKRIFLID 369
                  +++  G +GYPNVGKSS+IN L  + +C  AP PG T+  +++ L K + L+D
Sbjct: 213 REKGLLPRSVRAGIIGYPNVGKSSLINRLLKRKICAAAPRPGVTREMKWVKLGKDLDLLD 272

Query: 370 CPGVVYQNSDTE 371
            PG++    D +
Sbjct: 273 SPGMLPMRIDDQ 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2XGQ13.7e-19882.19Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. indica OX=39946 GN=NUG2 PE=3 S... [more]
Q10LF73.7e-19882.19Nuclear/nucleolar GTPase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NUG2 PE=1... [more]
Q9C9234.6e-19382.52Nuclear/nucleolar GTPase 2 OS=Arabidopsis thaliana OX=3702 GN=NUG2 PE=1 SV=1[more]
Q99LH11.8e-12053.11Nucleolar GTP-binding protein 2 OS=Mus musculus OX=10090 GN=Gnl2 PE=1 SV=2[more]
Q138236.8e-12052.87Nucleolar GTP-binding protein 2 OS=Homo sapiens OX=9606 GN=GNL2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KTZ37.3e-234100.00Nuclear/nucleolar GTPase 2 OS=Cucumis sativus OX=3659 GN=Csa_5G631500 PE=3 SV=1[more]
A0A5D3BS648.1e-23399.51Nuclear/nucleolar GTPase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1K2N03.2e-22194.66Nuclear/nucleolar GTPase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489529 PE=3 SV=1[more]
A0A6J1FGU62.1e-22094.17Nuclear/nucleolar GTPase 2 OS=Cucurbita moschata OX=3662 GN=LOC111445557 PE=3 SV... [more]
A0A6J1C0B43.9e-21993.69Nuclear/nucleolar GTPase 2 OS=Momordica charantia OX=3673 GN=LOC111006286 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_004142347.11.5e-233100.00nuclear/nucleolar GTPase 2 [Cucumis sativus] >KGN52399.1 hypothetical protein Cs... [more]
TYK01950.11.7e-23299.51nuclear/nucleolar GTPase 2 [Cucumis melo var. makuwa][more]
XP_038890331.11.1e-22898.30nuclear/nucleolar GTPase 2 [Benincasa hispida] >XP_038890332.1 nuclear/nucleolar... [more]
XP_023550727.16.6e-22194.66nuclear/nucleolar GTPase 2 [Cucurbita pepo subsp. pepo] >XP_023550729.1 nuclear/... [more]
XP_022993572.16.6e-22194.66nuclear/nucleolar GTPase 2 [Cucurbita maxima] >XP_022993573.1 nuclear/nucleolar ... [more]
Match NameE-valueIdentityDescription
AT1G52980.13.3e-19482.52GTP-binding family protein [more]
AT3G07050.17.2e-4035.56GTP-binding family protein [more]
AT2G27200.14.1e-2729.89P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G08410.17.7e-2629.43P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G02790.11.8e-1428.65GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..171
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR11089GTP-BINDING PROTEIN-RELATEDcoord: 2..413
NoneNo IPR availableCDDcd01858NGP_1coord: 206..362
e-value: 1.33829E-117
score: 337.734
IPR006073GTP binding domainPRINTSPR00326GTP1OBGcoord: 310..330
score: 46.14
coord: 356..371
score: 26.08
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 310..371
e-value: 1.7E-14
score: 53.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 194..364
e-value: 1.4E-44
score: 153.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 200..370
IPR012971Nucleolar GTP-binding protein 2, N-terminal domainPFAMPF08153NGP1NTcoord: 42..167
e-value: 3.9E-47
score: 159.6
IPR024929Nucleolar GTP-binding protein 2PANTHERPTHR11089:SF9NUCLEOLAR GTP-BINDING PROTEIN 2coord: 2..413
IPR030378Circularly permuted (CP)-type guanine nucleotide-binding (G) domainPROSITEPS51721G_CPcoord: 204..365
score: 36.55735

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI05G28360CSPI05G28360gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G28360.2.utr3p1CSPI05G28360.2.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G28360.2.cds10CSPI05G28360.2.cds10CDS
CSPI05G28360.2.cds9CSPI05G28360.2.cds9CDS
CSPI05G28360.2.cds8CSPI05G28360.2.cds8CDS
CSPI05G28360.2.cds7CSPI05G28360.2.cds7CDS
CSPI05G28360.2.cds6CSPI05G28360.2.cds6CDS
CSPI05G28360.2.cds5CSPI05G28360.2.cds5CDS
CSPI05G28360.2.cds4CSPI05G28360.2.cds4CDS
CSPI05G28360.2.cds3CSPI05G28360.2.cds3CDS
CSPI05G28360.2.cds2CSPI05G28360.2.cds2CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G28360.2.utr5p1CSPI05G28360.2.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI05G28360.2CSPI05G28360.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042254 ribosome biogenesis
cellular_component GO:0005730 nucleolus
molecular_function GO:0005525 GTP binding
molecular_function GO:0003924 GTPase activity
molecular_function GO:0003729 mRNA binding