CSPI05G27260.2 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI05G27260.2
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionUbiquitin-conjugating enzyme E2 C, putative
LocationChr5: 25839252 .. 25841695 (-)
Sequence length1280
RNA-Seq ExpressionCSPI05G27260.2
SyntenyCSPI05G27260.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAGAGTAGAAAAGGAAAGGGAAATTATATAAAAAAAGTATAACATTTCACGAAATATATATATTTTAGTTTTGGTTTTTGTATTATTTAATTGGCTTCGTTTCGCCGAAATAAACAAAACTAAACATCGCTGCGTATAAAGCCACCGTCCGTCTTCATCCCCACCTTCTCCACTAGCCCAACAAATTTTCTCCACAAAGAAGAAAGTGAAATATCACTGATTAAACTCATCATTGAATTCAAATCTTTTTGAATTTAAGAACGACCCTTTTTTGCATTTCATCCTCCCTTTGTTTACTTCGTAGTTGGATCTCATTGTGGGCTTGGAGGATTTTTCTTTCTGGGGACTGTTATTGATCCTTGTGTTGTGGATATGGAAGTCAGACCCAATCCGACACCCGAAAACTCGTCCATGTCGCAGCATCATCATCTAATCAGTTCTTCATTAAAGCAGCCTGCCGCTTCTTCCAATCCTATCGATTCCAACGCTGTTGCACAGAGGTGATTCTTCTTTTTCATCATTCTTTTGGTTTATTTATTGTTTCGTTCCCGGGATTTATGGAGAATGTAAAAAATGGACAAGAAAGGATTGAAAACGATAGCAAGAATTCAGGGACGTACGATTTGTTGTTACTTTCTAGTTTTTTTTTTTTTTTTTTTACACTATTCTGTTATGTTAATTATTGCTTGCTTTTCCATGTTTTGATTTGATTATCAAATCTGTAGCAGGCTTCAGATTTACGGATTCTTATTAATTGCGGATTATAGTTGTGGGGTTTTGACATTTTTTTCTTTATTTGGCTGCAGGCTGCAAAAGGAGTTGATGTCACTGATGGTATGTTTCTTTCTGCTTTTACATTTCTTCTTGAATTTTTGGTACTATTGGGTCATCTTGAGTCGGTATCCATGTCATTTAAGTTGGTTGAAACATTGAGATTTGAGAATAAGCAATTTTGGATTTCTCCCTGCAGAGTGGGGACTGATTTAATTTGGTGGTCAATATGACAAGATTTGGAAAATATGTAATTTTACTGCTCTTCCCTTTTGTTGTGGCATCATGGTCCCATCCACAATCCCTGATTTCTTTTTAGAGTAAGTGTAGTTTGTACCTGACGTCGGTTCTTCGAACTTTCACATTCTGTTTTGGCAGATACCATGCTTCTTGATATCTTGTATCCAAGATCATTAGGCAAATAATGGCATCTTGGATTTCAACTTTGCATTTCTTTGTATTTGATAATGCTGAATATTTGTTTCTACAATGTCTTGTTACGCCATAATATTGTGTTTTACTAGGTTTGTTGTTTCGTAGAAAGATCATTCCTGGTTGTTGAATGGGGTTTTGCAGCCATGAGATGGTTATAAATTGCCTGTCTTCTGGATAGTTCATTAACCATATTTATTTGGTGATGCTCTCTACTATAGATGAGTGGAGGTGATCTAGGTGTATCAGCTTTCCCAGATGGTGAGAGCATTTTCACATGGATAGGCACCATTGAGGGTGGGAAAGGAACTTCATACGAGGGTTTGTCCTACAAACTCTCCTTACATTTTCCAATGGAGTACCCTTTCAAGCCACCGCTTGTTAAGTTCGAGACGATGTGCTTCCATCCAAATGTTGATCAATATGGCAACATTTGTCTCGATATTCTTCAGGTTTCAGTTAAATATACAGTGGTGTTTTTGCTTCCCTATGTTTTGGATGGTTATTGTACAATCCATATCCTTTGTTGTTGTTTACTATTGAAGTTTGTTAGGGTGTATAACACACCACAGACAGTTTAGTTGGTTTTTCGAACCTCATCCTTATATTTGATGTAATTTCCTCTTTTTTGATAGGACAAGTGGTCTTCCGCTTATGATTGCAGAACCATCCTTTTGTCTATTCAAAGCCTATTAGGAGGTTTGCTCGTTCCTGCCAACAAATTTATCAATTTTCTATTTTTGATCCTCACCCTGATGAAGTGTTGTGTTTCCTTGCAGAGCCGAACCCAGAGAGTCCTCTCAACAGCTATGCTGCTGCTCTGTGGAATAACAAGGAAGGTTGGACTCAAGTCCCTCCCCTTGTGTCCCATCATTTTGTTTTAATGGCGATTCTTTTCTGATCACTTATTTTCTTGTTGTCCAGATTACAGAAAGATGGTCCACAAACAGTATTTTTCTGGTGCAGTCTTAGACGGCTGATTTGGAAAAGCCTAAGTAGAAAACAAATGATTGACACCATATCGTCAGTTTGGCATACATTAAAATTACTGTAAAGCGTCGAGATAAAAAGGTCTCAAACTTCATAACATAGAGAGAAAGAGAAAGAGAGATTATAGTTTACATATGAACTGGATATTTGTACCATTTGCCCATGATTGTTATATTGGAAGTATGAACTTTTACGTATTTTAACCTCCATAGCTAATTCTATTATTCCGTATTTAATTGCTGGGCGCT

mRNA sequence

AGAAAGAGTAGAAAAGGAAAGGGAAATTATATAAAAAAAGTATAACATTTCACGAAATATATATATTTTAGTTTTGGTTTTTGTATTATTTAATTGGCTTCGTTTCGCCGAAATAAACAAAACTAAACATCGCTGCGTATAAAGCCACCGTCCGTCTTCATCCCCACCTTCTCCACTAGCCCAACAAATTTTCTCCACAAAGAAGAAAGTGAAATATCACTGATTAAACTCATCATTGAATTCAAATCTTTTTGAATTTAAGAACGACCCTTTTTTGCATTTCATCCTCCCTTTGTTTACTTCGTAGTTGGATCTCATTGTGGGCTTGGAGGATTTTTCTTTCTGGGGACTGTTATTGATCCTTGTGTTGTGGATATGGAAGTCAGACCCAATCCGACACCCGAAAACTCGTCCATGTCGCAGCATCATCATCTAATCAGTTCTTCATTAAAGCAGCCTGCCGCTTCTTCCAATCCTATCGATTCCAACGCTGTTGCACAGAGGCTGCAAAAGGAGTTGATGTCACTGATGATGAGTGGAGGTGATCTAGGTGTATCAGCTTTCCCAGATGGTGAGAGCATTTTCACATGGATAGGCACCATTGAGGGTGGGAAAGGAACTTCATACGAGGGTTTGTCCTACAAACTCTCCTTACATTTTCCAATGGAGTACCCTTTCAAGCCACCGCTTGTTAAGTTCGAGACGATGTGCTTCCATCCAAATGTTGATCAATATGGCAACATTTGTCTCGATATTCTTCAGGACAAGTGGTCTTCCGCTTATGATTGCAGAACCATCCTTTTGTCTATTCAAAGCCTATTAGGAGGTTTGCTCGTTCCTGCCAACAAATTTATCAATTTTCTATTTTTGATCCTCACCCTGATGAAGTGTTGTGTTTCCTTGCAGAGCCGAACCCAGAGAGTCCTCTCAACAGCTATGCTGCTGCTCTGTGGAATAACAAGGAAGATTACAGAAAGATGGTCCACAAACAGTATTTTTCTGGTGCAGTCTTAGACGGCTGATTTGGAAAAGCCTAAGTAGAAAACAAATGATTGACACCATATCGTCAGTTTGGCATACATTAAAATTACTGTAAAGCGTCGAGATAAAAAGGTCTCAAACTTCATAACATAGAGAGAAAGAGAAAGAGAGATTATAGTTTACATATGAACTGGATATTTGTACCATTTGCCCATGATTGTTATATTGGAAGTATGAACTTTTACGTATTTTAACCTCCATAGCTAATTCTATTATTCCGTATTTAATTGCTGGGCGCT

Coding sequence (CDS)

ATGGAAGTCAGACCCAATCCGACACCCGAAAACTCGTCCATGTCGCAGCATCATCATCTAATCAGTTCTTCATTAAAGCAGCCTGCCGCTTCTTCCAATCCTATCGATTCCAACGCTGTTGCACAGAGGCTGCAAAAGGAGTTGATGTCACTGATGATGAGTGGAGGTGATCTAGGTGTATCAGCTTTCCCAGATGGTGAGAGCATTTTCACATGGATAGGCACCATTGAGGGTGGGAAAGGAACTTCATACGAGGGTTTGTCCTACAAACTCTCCTTACATTTTCCAATGGAGTACCCTTTCAAGCCACCGCTTGTTAAGTTCGAGACGATGTGCTTCCATCCAAATGTTGATCAATATGGCAACATTTGTCTCGATATTCTTCAGGACAAGTGGTCTTCCGCTTATGATTGCAGAACCATCCTTTTGTCTATTCAAAGCCTATTAGGAGGTTTGCTCGTTCCTGCCAACAAATTTATCAATTTTCTATTTTTGATCCTCACCCTGATGAAGTGTTGTGTTTCCTTGCAGAGCCGAACCCAGAGAGTCCTCTCAACAGCTATGCTGCTGCTCTGTGGAATAACAAGGAAGATTACAGAAAGATGGTCCACAAACAGTATTTTTCTGGTGCAGTCTTAG

Protein sequence

MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGGLLVPANKFINFLFLILTLMKCCVSLQSRTQRVLSTAMLLLCGITRKITERWSTNSIFLVQS*
Homology
[Close]
BLAST of CSPI05G27260.2 vs. ExPASy Swiss-Prot
Match: Q8L7T3 (Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.8e-44
Identity = 89/129 (68.99%), Postives = 101/129 (78.29%), Query Frame = 0 

Query: 22  SSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKG 81
           S+  KQPAA +  +DS +V +RLQ ELM LMM GG  G+SAFP+ ++IF W GTI G K 
Sbjct: 18  SNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGP-GISAFPEEDNIFCWKGTITGSKD 77

Query: 82  TSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTI 141
           T +EG  Y+LSL F  +YPFKPP VKFET CFHPNVD YGNICLDILQDKWSSAYD RTI
Sbjct: 78  TVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICLDILQDKWSSAYDVRTI 137

Query: 142 LLSIQSLLG 151
           LLSIQSLLG
Sbjct: 138 LLSIQSLLG 145

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy Swiss-Prot
Match: Q9LJZ5 (Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-41
Identity = 85/125 (68.00%), Postives = 97/125 (77.60%), Query Frame = 0 

Query: 26  KQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYE 85
           KQ A  +  +DS++V +RLQ ELM LMM G D G+SAFP+ ++IF W GTI G K T +E
Sbjct: 23  KQSAPPTKTVDSHSVLKRLQSELMGLMM-GADPGISAFPEEDNIFCWKGTITGSKDTVFE 82

Query: 86  GLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSI 145
           G  Y+LSL F  +YPFK P VKFET CFHPNVD YGNICLDILQDKWSSAYD RTILLSI
Sbjct: 83  GTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSI 142

Query: 146 QSLLG 151
           QSLLG
Sbjct: 143 QSLLG 146

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy Swiss-Prot
Match: Q54XS6 (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 GN=ube2c PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 6.7e-39
Identity = 74/113 (65.49%), Postives = 93/113 (82.30%), Query Frame = 0 

Query: 38  NAVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPM 97
           ++V++RLQ ELM+LMM G   G+SAFPDG++IF WIGTI+G K T YE + +K+SL FP 
Sbjct: 5   HSVSKRLQSELMNLMM-GPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKFPT 64

Query: 98  EYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           +YP+KPP VKFET CFHPNVD  GNICLDIL+DKWS  Y+ R++L+SIQSLLG
Sbjct: 65  DYPYKPPTVKFETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLG 116

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy Swiss-Prot
Match: P56616 (Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevis OX=8355 GN=ube2c PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.7e-39
Identity = 76/112 (67.86%), Postives = 91/112 (81.25%), Query Frame = 0 

Query: 39  AVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPME 98
           +V +RLQ+ELM+LMMS GD G+SAFP+ +++F WIGTI+G  GT YE L YKLSL FP  
Sbjct: 30  SVGKRLQQELMTLMMS-GDKGISAFPESDNLFKWIGTIDGAVGTVYEDLRYKLSLEFPSG 89

Query: 99  YPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           YP+  P VKF T CFHPNVD +GNICLDIL+DKWS+ YD RTILLS+QSLLG
Sbjct: 90  YPYNAPTVKFVTPCFHPNVDSHGNICLDILKDKWSALYDVRTILLSLQSLLG 140

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy Swiss-Prot
Match: Q95044 (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 5.6e-38
Identity = 71/125 (56.80%), Postives = 101/125 (80.80%), Query Frame = 0 

Query: 26  KQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYE 85
           ++P   +   + ++V++RLQ+EL +L+MS GD G++AFPDG+++F W+ T++G K T YE
Sbjct: 17  ERPRDMTTSKERHSVSKRLQQELRTLLMS-GDPGITAFPDGDNLFKWVATLDGPKDTVYE 76

Query: 86  GLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSI 145
            L YKL+L FP +YP+KPP+VKF T C+HPNVDQ GNICLDIL++ W+++YD RTILLS+
Sbjct: 77  SLKYKLTLEFPSDYPYKPPVVKFTTPCWHPNVDQSGNICLDILKENWTASYDVRTILLSL 136

Query: 146 QSLLG 151
           QSLLG
Sbjct: 137 QSLLG 140

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy TrEMBL
Match: A0A6J1HPL4 (probable ubiquitin-conjugating enzyme E2 C isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466553 PE=3 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.4e-86
Identity = 173/212 (81.60%), Postives = 181/212 (85.38%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ  HL SSSLKQP+ASSNPIDSNAVAQRLQKELMSLMMSG DLGV
Sbjct: 1   MEVRPNPTGDNSSISQ-QHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTIEGGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGGLLVPANKFINFLFLILTLMKCCVSLQSRT 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLG +                      ++QS T
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGVVF---------------------TMQSPT 180

Query: 181 QRVLSTAMLLLCGITRKITERWSTNSIFLVQS 213
           QRVLSTAMLLL GITRKITERWSTNSIF VQS
Sbjct: 181 QRVLSTAMLLLFGITRKITERWSTNSIFWVQS 190

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy TrEMBL
Match: A0A6J1G1M1 (probable ubiquitin-conjugating enzyme E2 C isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449838 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.7e-85
Identity = 171/212 (80.66%), Postives = 181/212 (85.38%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ  HL SSSLKQP+ASSNPIDSNAVAQRLQKELMSLMMSG DLGV
Sbjct: 1   MEVRPNPTGDNSSISQ-QHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTIEGGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFET CFHPNVDQY
Sbjct: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETTCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGGLLVPANKFINFLFLILTLMKCCVSLQSRT 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLG +                      ++QS T
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGVVF---------------------TMQSPT 180

Query: 181 QRVLSTAMLLLCGITRKITERWSTNSIFLVQS 213
           QRVLSTAMLLL GIT+KITE+WSTNSIFLVQS
Sbjct: 181 QRVLSTAMLLLFGITKKITEKWSTNSIFLVQS 190

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy TrEMBL
Match: A0A0A0KV09 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623460 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.6e-80
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           GNICLDILQDKWSSAYDCRTILLSIQSLLG
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 150

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy TrEMBL
Match: A0A5D3CDL5 (Putative ubiquitin-conjugating enzyme E2 C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002790 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 5.2e-79
Identity = 147/150 (98.00%), Postives = 150/150 (100.00%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPTPENSS+SQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSVSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFPDGESIFTWIGTI+GGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIQGGKGTTYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           GNICLDILQDKWSSAYDCRTILLSIQSLLG
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 150

[Close]
BLAST of CSPI05G27260.2 vs. ExPASy TrEMBL
Match: A0A1S3BH18 (probable ubiquitin-conjugating enzyme E2 C OS=Cucumis melo OX=3656 GN=LOC103489475 PE=3 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 5.2e-79
Identity = 147/150 (98.00%), Postives = 150/150 (100.00%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPTPENSS+SQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSVSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFPDGESIFTWIGTI+GGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIQGGKGTTYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           GNICLDILQDKWSSAYDCRTILLSIQSLLG
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 150

[Close]
BLAST of CSPI05G27260.2 vs. NCBI nr
Match: XP_022967027.1 (probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucurbita maxima])

HSP 1 Score: 327.8 bits (839), Expect = 7.0e-86
Identity = 173/212 (81.60%), Postives = 181/212 (85.38%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ  HL SSSLKQP+ASSNPIDSNAVAQRLQKELMSLMMSG DLGV
Sbjct: 1   MEVRPNPTGDNSSISQ-QHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTIEGGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGGLLVPANKFINFLFLILTLMKCCVSLQSRT 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLG +                      ++QS T
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGVVF---------------------TMQSPT 180

Query: 181 QRVLSTAMLLLCGITRKITERWSTNSIFLVQS 213
           QRVLSTAMLLL GITRKITERWSTNSIF VQS
Sbjct: 181 QRVLSTAMLLLFGITRKITERWSTNSIFWVQS 190

[Close]
BLAST of CSPI05G27260.2 vs. NCBI nr
Match: XP_022945674.1 (probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucurbita moschata])

HSP 1 Score: 325.5 bits (833), Expect = 3.5e-85
Identity = 171/212 (80.66%), Postives = 181/212 (85.38%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ  HL SSSLKQP+ASSNPIDSNAVAQRLQKELMSLMMSG DLGV
Sbjct: 1   MEVRPNPTGDNSSISQ-QHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTIEGGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFET CFHPNVDQY
Sbjct: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETTCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGGLLVPANKFINFLFLILTLMKCCVSLQSRT 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLG +                      ++QS T
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGVVF---------------------TMQSPT 180

Query: 181 QRVLSTAMLLLCGITRKITERWSTNSIFLVQS 213
           QRVLSTAMLLL GIT+KITE+WSTNSIFLVQS
Sbjct: 181 QRVLSTAMLLLFGITKKITEKWSTNSIFLVQS 190

[Close]
BLAST of CSPI05G27260.2 vs. NCBI nr
Match: XP_004142430.1 (probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucumis sativus] >KGN52282.1 hypothetical protein Csa_008141 [Cucumis sativus])

HSP 1 Score: 307.8 bits (787), Expect = 7.5e-80
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           GNICLDILQDKWSSAYDCRTILLSIQSLLG
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 150

[Close]
BLAST of CSPI05G27260.2 vs. NCBI nr
Match: XP_008446898.1 (PREDICTED: probable ubiquitin-conjugating enzyme E2 C [Cucumis melo] >KAA0034731.1 putative ubiquitin-conjugating enzyme E2 C [Cucumis melo var. makuwa] >TYK09284.1 putative ubiquitin-conjugating enzyme E2 C [Cucumis melo var. makuwa])

HSP 1 Score: 303.9 bits (777), Expect = 1.1e-78
Identity = 147/150 (98.00%), Postives = 150/150 (100.00%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPTPENSS+SQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSVSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFPDGESIFTWIGTI+GGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIQGGKGTTYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           GNICLDILQDKWSSAYDCRTILLSIQSLLG
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLG 150

[Close]
BLAST of CSPI05G27260.2 vs. NCBI nr
Match: XP_023542479.1 (probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 294.3 bits (752), Expect = 8.6e-76
Identity = 156/197 (79.19%), Postives = 168/197 (85.28%), Query Frame = 0 

Query: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ  HL SSSLKQP+ASSNPIDSNAVAQRLQKELMSLMMSG DLGV
Sbjct: 1   MEVRPNPTGDNSSISQ-QHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGV 60

Query: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTIEGGKGT+YEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGGLLVPANKFINFLFLILTL---MKCCVSLQ 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLGGLL+    F N + L++ L   +K C  LQ
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGGLLISPCIFGNIMILMIILSSHVKLC--LQ 180

Query: 181 SRTQRVLSTAMLLLCGI 195
            R Q   S+  L  C +
Sbjct: 181 CRVQPRESSQQLCCCSL 194

[Close]
BLAST of CSPI05G27260.2 vs. TAIR 10
Match: AT1G50490.1 (ubiquitin-conjugating enzyme 20 )

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-45
Identity = 89/129 (68.99%), Postives = 101/129 (78.29%), Query Frame = 0 

Query: 22  SSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKG 81
           S+  KQPAA +  +DS +V +RLQ ELM LMM GG  G+SAFP+ ++IF W GTI G K 
Sbjct: 18  SNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGP-GISAFPEEDNIFCWKGTITGSKD 77

Query: 82  TSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTI 141
           T +EG  Y+LSL F  +YPFKPP VKFET CFHPNVD YGNICLDILQDKWSSAYD RTI
Sbjct: 78  TVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICLDILQDKWSSAYDVRTI 137

Query: 142 LLSIQSLLG 151
           LLSIQSLLG
Sbjct: 138 LLSIQSLLG 145

[Close]
BLAST of CSPI05G27260.2 vs. TAIR 10
Match: AT3G20060.1 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 171.4 bits (433), Expect = 7.8e-43
Identity = 85/125 (68.00%), Postives = 97/125 (77.60%), Query Frame = 0 

Query: 26  KQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYE 85
           KQ A  +  +DS++V +RLQ ELM LMM G D G+SAFP+ ++IF W GTI G K T +E
Sbjct: 23  KQSAPPTKTVDSHSVLKRLQSELMGLMM-GADPGISAFPEEDNIFCWKGTITGSKDTVFE 82

Query: 86  GLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSI 145
           G  Y+LSL F  +YPFK P VKFET CFHPNVD YGNICLDILQDKWSSAYD RTILLSI
Sbjct: 83  GTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSI 142

Query: 146 QSLLG 151
           QSLLG
Sbjct: 143 QSLLG 146

[Close]
BLAST of CSPI05G27260.2 vs. TAIR 10
Match: AT3G20060.2 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 152.1 bits (383), Expect = 4.9e-37
Identity = 74/102 (72.55%), Postives = 81/102 (79.41%), Query Frame = 0 

Query: 49  MSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKF 108
           M LMM G D G+SAFP+ ++IF W GTI G K T +EG  Y+LSL F  +YPFK P VKF
Sbjct: 1   MGLMM-GADPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKF 60

Query: 109 ETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLG 151
           ET CFHPNVD YGNICLDILQDKWSSAYD RTILLSIQSLLG
Sbjct: 61  ETCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSIQSLLG 101

[Close]
BLAST of CSPI05G27260.2 vs. TAIR 10
Match: AT2G16740.1 (ubiquitin-conjugating enzyme 29 )

HSP 1 Score: 102.4 bits (254), Expect = 4.5e-22
Identity = 52/108 (48.15%), Postives = 67/108 (62.04%), Query Frame = 0 

Query: 42  QRLQKELMSLMMSGGDLGVSAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPF 101
           +R+ KEL  L      +  SA P GE +F W  TI G   + Y G  + +++HFP +YPF
Sbjct: 4   RRILKELKELQRD-PPVSCSAGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDYPF 63

Query: 102 KPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLL 150
           KPP V F T  FHPN++  GNICLDIL+D+WS A     +LLSI SLL
Sbjct: 64  KPPKVVFRTKVFHPNINSNGNICLDILKDQWSPALTISKVLLSICSLL 110

[Close]
BLAST of CSPI05G27260.2 vs. TAIR 10
Match: AT5G62540.1 (ubiquitin-conjugating enzyme 3 )

HSP 1 Score: 97.1 bits (240), Expect = 1.9e-20
Identity = 44/92 (47.83%), Postives = 62/92 (67.39%), Query Frame = 0 

Query: 58  LGVSAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNV 117
           +G+S  P   +I  W   I G + T ++G ++KL+LHF  +YP KPP+V+F +  FHPN+
Sbjct: 22  VGISGAPQDNNIMHWNALIFGPEDTPWDGGTFKLTLHFTEDYPNKPPIVRFVSRMFHPNI 81

Query: 118 DQYGNICLDILQDKWSSAYDCRTILLSIQSLL 150
              G+ICLDILQ++WS  YD   +L SIQSLL
Sbjct: 82  YADGSICLDILQNQWSPIYDVAAVLTSIQSLL 113

The following BLAST results are available for this feature:
BLAST of CSPI05G27260.2 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8L7T31.8e-4468.99Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2... [more]
Q9LJZ51.1e-4168.00Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1... [more]
Q54XS66.7e-3965.49Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 ... [more]
P566168.7e-3967.86Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevis OX=8355 GN=ube2c PE=1 SV=1[more]
Q950445.6e-3856.80Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 S... [more]
BLAST of CSPI05G27260.2 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HPL43.4e-8681.60probable ubiquitin-conjugating enzyme E2 C isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1G1M11.7e-8580.66probable ubiquitin-conjugating enzyme E2 C isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A0A0KV093.6e-80100.00UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623460 PE... [more]
A0A5D3CDL55.2e-7998.00Putative ubiquitin-conjugating enzyme E2 C OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BH185.2e-7998.00probable ubiquitin-conjugating enzyme E2 C OS=Cucumis melo OX=3656 GN=LOC1034894... [more]
BLAST of CSPI05G27260.2 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022967027.17.0e-8681.60probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucurbita maxima][more]
XP_022945674.13.5e-8580.66probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucurbita moschata][more]
XP_004142430.17.5e-80100.00probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucumis sativus] >KGN5228... [more]
XP_008446898.11.1e-7898.00PREDICTED: probable ubiquitin-conjugating enzyme E2 C [Cucumis melo] >KAA0034731... [more]
XP_023542479.18.6e-7679.19probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucurbita pepo subsp. pep... [more]
BLAST of CSPI05G27260.2 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50490.11.3e-4568.99ubiquitin-conjugating enzyme 20 [more]
AT3G20060.17.8e-4368.00ubiquitin-conjugating enzyme19 [more]
AT3G20060.24.9e-3772.55ubiquitin-conjugating enzyme19 [more]
AT2G16740.14.5e-2248.15ubiquitin-conjugating enzyme 29 [more]
AT5G62540.11.9e-2047.83ubiquitin-conjugating enzyme 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 42..198
e-value: 2.5E-33
score: 126.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..35
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 22..151
NoneNo IPR availablePANTHERPTHR24068:SF320UBIQUITIN-CONJUGATING ENZYME E2 C-RELATEDcoord: 22..151
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 43..149
e-value: 1.7E-33
score: 115.2
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 39..189
score: 33.50111
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 42..149
e-value: 7.06331E-48
score: 151.971
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 33..160
e-value: 4.7E-42
score: 145.2
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 36..150
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 113..128

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI05G27260CSPI05G27260gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G27260.2.utr3p1CSPI05G27260.2.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G27260.2.cds5CSPI05G27260.2.cds5CDS
CSPI05G27260.2.cds4CSPI05G27260.2.cds4CDS
CSPI05G27260.2.cds3CSPI05G27260.2.cds3CDS
CSPI05G27260.2.cds2CSPI05G27260.2.cds2CDS
CSPI05G27260.2.cds1CSPI05G27260.2.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G27260.2.utr5p1CSPI05G27260.2.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI05G27260.2CSPI05G27260.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0006952 defense response
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
biological_process GO:0009607 response to biotic stimulus
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity