CSPI04G24590.2 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G24590.2
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cytochrome P450
Location	Chr4: 22286853 .. 22289524 (+)
Sequence length	1795
RNA-Seq Expression	CSPI04G24590.2
Synteny	CSPI04G24590.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTTCGTGACTTGAGGTTTTATGGGTTTTTGGTTTTCACCCAACCAAGAAAGCTAATGTTTCTTCTTCGGATGGAAGATAGTTCATGGATGGTAACGTTTTTCTTTTTTAATTCATTTAACATTTTAGTTTTGAAATTTTCAGTATTGATACTGTTTTTGAGATTTTGTTGTAGAGGACATTCAGTTTACAATGAATAATGTAAATATTATCTTCTTTGTTCATATATCTAAGTTTGTCTTCCAGGATTCTCTTTCACTAAAGATTCATTTTTTTCATTAATTATTTAGTTTATATGAAATCTGAATCAATTACAAGGAATCACAGAAGCGATTACTCACGAGGTCAGTTTATCTAATTTTAATTTACGGAGATCCATATAACTTTTTTTTTCTTTCAATTATGGATTTCAATTTTGTTCATTTTCTTTCTACATTTTTTTTTTGTCAATAATTTACTCTTTTACAACTTGTATATAATTGGCCGTATATTTCACCATACAAATTATATACAATTATATATCCATTATTTCTTTCTTCTTGTTTTAAAATAATATAACTTTCTATTAAATTTTTTATAGCGTAAACAAAATGTTAGATCACATAAATTTAGTTTTTTTAATTTCCATCAATGCCATTTTTTAGTTTAAACTTAGAGGTAAAAACAAATTTAATAGAATGTATAACACTCACCTATATTTAAGTATTATCTAAGTTTTTGAGTGATGATAAATTTAATATAACATCCAGTGTAACTAAATTGCCGAATTGACTTGCAGGTAAGAAAGCAACTTGAAAAATTTAAACGGAACTCAATGATACTTCGACCAACCCAACTAGCATGGTTATAATTTACAAATGATTCTCGCAGGTAAATTTCATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTAATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGAGTTAACTTGATTTCTCTCTTAGTTATTCAAAAGACGTAACCAAATAGATTTTGAAACACTATTTTAAACACATATGAAAATATATGTAATCCGACTCTATTTTTCCTTAAATCAACCAAATCGTCCCAAATGAAATTGGATTGTTGTAGGTGTTGCTATTAGCCGGAACGGAAGGTTCCATCAATACAATGGAGTGGCTTCTATCACTATTACTCAACCACCCACACTCTCTTCAACGAGCCCAAATGGAAATCGACGACGTAGTTGGAAGAACTAATCGGCTCCTGGAGGAATCAGATCTAACCCACCTTCCTTACCTCCGTAGCCTGATCCACGAAACTCTTCGAATGTACCCGCCAGGCCCATTACTGATTCCACACGAATCGTCGGAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGAGGAAATTTAACCCAGATAGATTCAGAGGGGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGGAGGAGGTGTCCCGGCGAGGGCTTGGGGCTTCGCGTCGTTGGATTGGTTGTAGGGTCGCTGATTCAATGCTTTGAATGGGAGTCAATGGACGGAGAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCGAAAGCTCTCCCCCTTCGCACCCTTTGCCGGCCACGTTCAAATGCCACACATTTGCTTTCCCAAATCTAATTCAAAATGCATGGTCTTGTTTATGTTGTTCAACAACAATTAATTCTGTACATAAATTTTACATATTTTAGTAGTAGTTGAAAACACCTACGTTATTTCCAATTTGTTGACGTCC

mRNA sequence

CTTCTTCGTGACTTGAGGTTTTATGGGTTTTTGGTTTTCACCCAACCAAGAAAGCTAATGTTTCTTCTTCGGATGGAAGATAGTTCATGGATGTTTATATGAAATCTGAATCAATTACAAGGAATCACAGAAGCGATTACTCACGAGGTAAATTTCATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTAATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGTTGCTATTAGCCGGAACGGAAGGTTCCATCAATACAATGGAGTGGCTTCTATCACTATTACTCAACCACCCACACTCTCTTCAACGAGCCCAAATGGAAATCGACGACGTAGTTGGAAGAACTAATCGGCTCCTGGAGGAATCAGATCTAACCCACCTTCCTTACCTCCGTAGCCTGATCCACGAAACTCTTCGAATGTACCCGCCAGGCCCATTACTGATTCCACACGAATCGTCGGAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGAGGAAATTTAACCCAGATAGATTCAGAGGGGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGGAGGAGGTGTCCCGGCGAGGGCTTGGGGCTTCGCGTCGTTGGATTGGTTGTAGGGTCGCTGATTCAATGCTTTGAATGGGAGTCAATGGACGGAGAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCGAAAGCTCTCCCCCTTCGCACCCTTTGCCGGCCACGTTCAAATGCCACACATTTGCTTTCCCAAATCTAATTCAAAATGCATGGTCTTGTTTATGTTGTTCAACAACAATTAATTCTGTACATAAATTTTACATATTTTAGTAGTAGTTGAAAACACCTACGTTATTTCCAATTTGTTGACGTCC

Coding sequence (CDS)

ATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTAATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGTTGCTATTAGCCGGAACGGAAGGTTCCATCAATACAATGGAGTGGCTTCTATCACTATTACTCAACCACCCACACTCTCTTCAACGAGCCCAAATGGAAATCGACGACGTAGTTGGAAGAACTAATCGGCTCCTGGAGGAATCAGATCTAACCCACCTTCCTTACCTCCGTAGCCTGATCCACGAAACTCTTCGAATGTACCCGCCAGGCCCATTACTGATTCCACACGAATCGTCGGAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGAGGAAATTTAACCCAGATAGATTCAGAGGGGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGGAGGAGGTGTCCCGGCGAGGGCTTGGGGCTTCGCGTCGTTGGATTGGTTGTAGGGTCGCTGATTCAATGCTTTGAATGGGAGTCAATGGACGGAGAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCGAAAGCTCTCCCCCTTCGCACCCTTTGCCGGCCACGTTCAAATGCCACACATTTGCTTTCCCAAATCTAA

Protein sequence

MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI*

Homology

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.3e-133
Identity = 248/511 (48.53%), Postives = 350/511 (68.49%), Query Frame = 0


Query: 1   MVIPFLHLLILFL-LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRR 60
           ++  FL +++L + L++F      R RNLPPSP L+LP+IG L+L+   LHR+L ++S++
Sbjct: 6   LLYTFLAVVLLSISLKLFP--VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKK 65

Query: 61  HGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGE 120
           +G +   Q G R VLVVSSP+AAE+C +KND++FANRP  + GKY+GYNYT+++ +PYGE
Sbjct: 66  YGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGE 125

Query: 121 HWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFN 180
           HWRNLRR++++EI S+  L    SIR DEV+ L+  LY +       V+M+++  E+ FN
Sbjct: 126 HWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFN 185

Query: 181 ITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIEN 240
           +TMRM+AGKRY+G+DV   S+EA  FR +  E F  +G +N GDF+  ++W+   K  E 
Sbjct: 186 VTMRMVAGKRYFGQDVD--SDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KNYEK 245

Query: 241 RLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDE 300
           ++ ++  + D ++Q LI E R   NN              TMI+ LLSLQ+ +P+YY D+
Sbjct: 246 KVSKISQEMDAFLQRLIHESRINKNNV-------------TMIDHLLSLQESQPEYYTDQ 305

Query: 301 YIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTH 360
            I+G+++VLLLAGT+ S  T+EW +SLLLNHP +L++A+ EI+  VG +NRL+EE DL  
Sbjct: 306 IIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVG-SNRLIEEQDLPK 365

Query: 361 LPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP 420
           L YL ++I ET R+ P  P+L+PHESS+DC V G+ VP GT+L VN WAI  DP  W EP
Sbjct: 366 LTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEP 425

Query: 421 RKFNPDRFRG-DGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECID 480
             F P+R  G + E  K MPFG GRR CPG GL  RVVGL +G+LIQCFEW+ +    ID
Sbjct: 426 TLFKPERHGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKID 485

Query: 481 MSEGGGLTLPKALPLRTLCRPRSNATHLLSQ 507
           M+EG GLT+PKA PL  LC+PR+    ++S+
Sbjct: 486 MAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 441.4 bits (1134), Expect = 1.4e-122
Identity = 223/495 (45.05%), Postives = 329/495 (66.46%), Query Frame = 0


Query: 7   HLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 66
           +L + F+  I       + +NLPP P  SLPIIG L+ LK PLHRT   +S ++G +   
Sbjct: 13  YLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 72

Query: 67  QFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRR 126
            FG R V+VVSS S  + C +KNDV+ ANRPR +SGKY+ YNYT+L    YGEHWRNLRR
Sbjct: 73  WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 132

Query: 127 ISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFNITMRMIA 186
           I++L++LS+HR+ + S IR DE + LI RL +  +     +++ +  +++ FN  MRMI+
Sbjct: 133 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMIS 192

Query: 187 GKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQ 246
           GKRYYGED   +  +EA +FR++  E  +LSG  N  DF+ +++++   + +E RL+++ 
Sbjct: 193 GKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLKDIS 252

Query: 247 IKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLM 306
            K D +++ LIEEHR K   A             TMI+ LL+LQ  +P+YY D+ I+GL 
Sbjct: 253 GKTDAFLRGLIEEHRTKKERA------------NTMIDHLLNLQDSQPEYYTDQIIKGLA 312

Query: 307 LVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRS 366
           L +LLAGT+ S  T+EW +S LLNHP  L++ + E+D  VG+ +RL++ESDL  L YL++
Sbjct: 313 LAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQ-DRLVDESDLPKLTYLKN 372

Query: 367 LIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPD 426
           +I+ETLR+Y P PLL+PH +S++C++GG+ VP  T++ +N WAI  DP +W E   F P+
Sbjct: 373 VINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPE 432

Query: 427 RFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEGGGL 486
           RF   GE  K + FG GRR CPGEGL +R + + +  LIQCF+W+ ++G+ ID++E  G 
Sbjct: 433 RFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGF 490

Query: 487 TLPKALPLRTLCRPR 498
           TL K +PL+ +C+ R
Sbjct: 493 TLTKLVPLKAMCKSR 490

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 5.7e-121
Identity = 232/502 (46.22%), Postives = 326/502 (64.94%), Query Frame = 0


Query: 5   FLHLLI-LFLLQIFTNHFLH---RIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 64
           +L L+I LF L I    F +   + +NLPP P   LPIIG L+ LK PLH T   +S+++
Sbjct: 5   YLSLIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKY 64

Query: 65  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 124
           G I    FG R V+VVSS + A++C +KND++ ANRP  ++GKY+GYN T++  +PYG+H
Sbjct: 65  GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 124

Query: 125 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRL----YNAENDVVDMRTEFFEVMFN 184
           WRNLRRI S+EILSSHRL +   IR DE+  LI++L    YN   + V++R  F E+ FN
Sbjct: 125 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMTFN 184

Query: 185 ITMRMIAGKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 244
             MRM++GKRYYG D   +  EEA  FR I +E   L G  N+GDFL  ++W     G+E
Sbjct: 185 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GLE 244

Query: 245 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 304
            RL+++  + D ++Q LI+EHR    N+             TMI+ LL+ QQ +P+YY D
Sbjct: 245 KRLKKISKRTDAFLQGLIDEHRFGKRNS------------NTMIDHLLTQQQSQPEYYTD 304

Query: 305 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLT 364
           + I+GLM+V+LLAGT+ S  T+EW +S LLNHP  +++A+ E+D  +G  +R ++E D++
Sbjct: 305 QIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGH-DRQVDEHDIS 364

Query: 365 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 424
            LPYL+S+++ETLR++   PLL+PH SSED  +GG+++P  T+L VN W I  DP +W +
Sbjct: 365 KLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSD 424

Query: 425 PRKFNPDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECID 484
           P  F P+RF  +GE  K + FG GRR CPGE L  R  GL +G LIQCFEW+ +  E ID
Sbjct: 425 PTCFKPERFEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKID 484

Query: 485 MSEGGGLTLPKALPLRTLCRPR 498
           M E  G+T  K   L  +C+ R
Sbjct: 485 MVEAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 2.8e-120
Identity = 231/500 (46.20%), Postives = 323/500 (64.60%), Query Frame = 0


Query: 6   LHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVF 65
           L L  +  ++I       R++NLPP P  ++PIIG L+ LK PLHRT + +S+ +G I  
Sbjct: 8   LSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 67

Query: 66  FQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 125
             FG R V+VVSSPS A +C +KND+I ANRPR ++GKY+ YNYT+L  A YG+HWRNLR
Sbjct: 68  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 127

Query: 126 RISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDV---------VDMRTEFFEVMFN 185
           RI+++++LS++RL +   +R DE   LI++L     DV         V++R    E+ FN
Sbjct: 128 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLL---KDVVSEGFGFTKVELRPRLTEMTFN 187

Query: 186 ITMRMIAGKRYYGED-VAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 245
             MRMI+GKRYYG+D   +  EEA +FREI  E   L G  N GDFL +++ V L   +E
Sbjct: 188 AMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD-NLE 247

Query: 246 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 305
            R + +  + + +++ LIEEHR+   N +S           TMI+ LL L + +P+YY D
Sbjct: 248 KRCKRIAKRSNAFLEGLIEEHRR--GNIHSDGG--------TMIDHLLKLSESQPEYYSD 307

Query: 306 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLT 365
             I+GL+  +LLAGT+ S  T+EW++S LLNHP  L++A+ E+D  +G+ N+L++E DL+
Sbjct: 308 HLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGK-NKLVDEQDLS 367

Query: 366 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 425
            LPYL+++I ETLR++PP PLL+PH SSEDC +G F+VP  T++  NVW I  DP  W +
Sbjct: 368 KLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWND 427

Query: 426 PRKFNPDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECID 485
              F P+RF  + E  K M FG GRR CPG  L  R VG  VG LIQCFEWE    E +D
Sbjct: 428 ALSFKPERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLD 487

Query: 486 MSEGGGLTLPKALPLRTLCR 496
           M EG G+T+P  +PLR +C+
Sbjct: 488 MMEGKGITMPMKIPLRAMCK 491

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.1e-119
Identity = 229/499 (45.89%), Postives = 320/499 (64.13%), Query Frame = 0


Query: 9   LILFLL--QIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 68
           L LFLL  ++F      R  NLPPSP  SLPI+G   LLK P+HR    +S+ HGPI   
Sbjct: 10  LALFLLAYKLFFTSKTKRF-NLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPIFSL 69

Query: 69  QFGFRSVLVVSSPSAAEDCLS-KNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 128
           QFG R  +V+SS S A  C + +ND+I +NRP  ++ KY+ YNYT++  APYG+HWRNLR
Sbjct: 70  QFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRNLR 129

Query: 129 RISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGK 188
           RI SLEILSS+RL     IR DE+  ++ RL    N  +++     ++ FN  +RM+ GK
Sbjct: 130 RICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKEIELEPLLSDLTFNNIVRMVTGK 189

Query: 189 RYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKR 248
           RYYG++V    EEA  F+++  +    SG  + GD+L  +K  G S   E +++ L    
Sbjct: 190 RYYGDEV-HNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS--FEKKVKALAEAM 249

Query: 249 DDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVL 308
           D+ +Q L+EE ++  +               TM+  LLSLQQ EP+YY D  I+GLML +
Sbjct: 250 DEILQRLLEECKRDKDG-------------NTMVNHLLSLQQNEPEYYTDVTIKGLMLGM 309

Query: 309 LLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIH 368
           ++AGT+ S  T+EW +S LLNHP +L++A++EID+ +G+  RL++E D+ +LPYL++++ 
Sbjct: 310 MIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQ-ERLIDEPDIANLPYLQNIVS 369

Query: 369 ETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRFR 428
           ET R+YP  PLL+P   +ED  VGG+ VP GTM+ VN WAI  DP +W EP KF P+RF 
Sbjct: 370 ETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKFKPERFN 429

Query: 429 GDGEG-------FKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSE 488
           G GEG        K MPFG GRR CPG GLG ++V L +GSLIQCF+W+ ++GE IDM+E
Sbjct: 430 G-GEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEAIDMTE 489

Query: 489 GGGLTLPKALPLRTLCRPR 498
             G+ + K +PL  LC+ R
Sbjct: 490 TPGMAMRKKIPLSALCQSR 489

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy TrEMBL
Match: A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 4.5e-294
Identity = 505/507 (99.61%), Postives = 506/507 (99.80%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420

Query: 421 PDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEGG 480
           PDRF GDGEGFKWMPFGAGRRRCPGEGLGLRV+GLVVGSLIQCFEWESMDGECIDMSEGG
Sbjct: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 508
           GLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy TrEMBL
Match: A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 3.9e-274
Identity = 474/508 (93.31%), Postives = 489/508 (96.26%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAY-SSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+ SSSSSIQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390

Query: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450

Query: 421 NPDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEG 480
           NPDRF+ DGEGFKWMPFGAGRRRCPGE LGLRVVGLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510

Query: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 508
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 538

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy TrEMBL
Match: A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.2e-245
Identity = 429/509 (84.28%), Postives = 452/509 (88.80%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+           + DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS----------KKDAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFRGDGEG--FKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSE 480
           P RF  +GEG  FKWMPFGAGRRRCPGEGLGLRVVGL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 508
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy TrEMBL
Match: A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.9e-244
Identity = 427/509 (83.89%), Postives = 451/509 (88.61%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLILF+L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTL +ISRRH
Sbjct: 1   MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDM+TEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVA  SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGEDVAE-SEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+           + DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS----------KKDAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFRGDGEG--FKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSE 480
           P RF  +GEG  FKWMPFGAGRRRCPGEGLGLRVVGL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 508
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of CSPI04G24590.2 vs. ExPASy TrEMBL
Match: A0A7J0EHR3 (Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g0007630 PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 4.6e-182
Identity = 323/510 (63.33%), Postives = 402/510 (78.82%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M   ++++ ++    I   HFLH+IRNLPPSPF +LPI G LYLLKTP+HRTLS IS  H
Sbjct: 1   MATLYVYIPLILAFYIIAKHFLHKIRNLPPSPFPALPIFGHLYLLKTPIHRTLSNISAHH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+  +FGFR VL+VSSPSAAE+CLSKND++FANRPRL++GK+LG NY +L WAPYG  
Sbjct: 61  GPILLLRFGFRPVLLVSSPSAAEECLSKNDIVFANRPRLLAGKHLGQNYNTLSWAPYGAR 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRI+++EILSSHRLQTLSSIR DEVR+LIRRL    ++ VDM+  FFE+M N+TMR
Sbjct: 121 WRNLRRIAAVEILSSHRLQTLSSIRADEVRSLIRRLARG-SEGVDMKAAFFELMLNVTMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGK YYGEDVA  +E A RFREI  ETF L+G +N GDFL V+KW+G S G+E RL  
Sbjct: 181 MIAGKTYYGEDVAE-AEAARRFREIVTETFVLAGASNFGDFLPVMKWIGAS-GLEKRLIA 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           L+ KRD++MQ +IEE R+++    S       + KKTM+EVLLSLQ+ EP++Y D+ IRG
Sbjct: 241 LREKRDEFMQEMIEEQRRRIGGGGSE------EKKKTMVEVLLSLQETEPEHYTDQMIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           +M VLL AGT+ S+ TMEW LSLLLN P++LQ+AQ EID  VG+ +RL++ESD+  LPYL
Sbjct: 301 MMSVLLAAGTDTSVGTMEWALSLLLNQPYALQKAQTEIDKQVGQ-HRLIDESDIADLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           R +I+ET+RMYP GPLL+PHESSEDC VGGFH+P GTML VN+WAIQNDP +WVEP KF 
Sbjct: 361 RCIINETMRMYPAGPLLVPHESSEDCMVGGFHIPRGTMLLVNMWAIQNDPKIWVEPNKFK 420

Query: 421 PDRFRG-DG--EGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMS 480
           P+RF G +G  +GFK++PFG+GRR CPGEGL LR+VGL +GSLIQCF+WE +  E +DM+
Sbjct: 421 PERFEGLEGARDGFKFVPFGSGRRVCPGEGLALRMVGLALGSLIQCFDWERVGKEMVDMT 480

Query: 481 EGGGLTLPKALPLRTLCRPRSNATHLLSQI 508
           EG GLTLPKA PL   CRPR    ++LSQI
Sbjct: 481 EGTGLTLPKARPLMAKCRPRLTMVNILSQI 500

[Close]

BLAST of CSPI04G24590.2 vs. NCBI nr
Match: XP_004141396.1 (cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [Cucumis sativus] >XP_031739532.1 cytochrome P450 81Q32 [Cucumis sativus] >KGN55248.1 hypothetical protein Csa_012192 [Cucumis sativus])

HSP 1 Score: 1019.6 bits (2635), Expect = 9.2e-294
Identity = 505/507 (99.61%), Postives = 506/507 (99.80%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420

Query: 421 PDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEGG 480
           PDRF GDGEGFKWMPFGAGRRRCPGEGLGLRV+GLVVGSLIQCFEWESMDGECIDMSEGG
Sbjct: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 508
           GLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507

[Close]

BLAST of CSPI04G24590.2 vs. NCBI nr
Match: XP_008452618.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 953.4 bits (2463), Expect = 8.1e-274
Identity = 474/508 (93.31%), Postives = 489/508 (96.26%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAY-SSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+ SSSSSIQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390

Query: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450

Query: 421 NPDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEG 480
           NPDRF+ DGEGFKWMPFGAGRRRCPGE LGLRVVGLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510

Query: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 508
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 538

[Close]

BLAST of CSPI04G24590.2 vs. NCBI nr
Match: XP_038899302.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 914.8 bits (2363), Expect = 3.2e-262
Identity = 448/507 (88.36%), Postives = 475/507 (93.69%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M IPFL+LL+LF+LQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI+ RH
Sbjct: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF+FGFR VLVVSSPSAAEDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAPYGEH
Sbjct: 65  GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILS HRLQTLSSIRVDEVRTLIRRLYNA+NDVVDMRTEFFE+MFNI MR
Sbjct: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAA +EE GRFREIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN+LRE
Sbjct: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ+KRD WMQSLIEEHRK+MNN   +S+S+Q DAKKTMIEVLLSLQQ EP YYKDE+IRG
Sbjct: 245 LQMKRDVWMQSLIEEHRKQMNN---TSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRG 304

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHP  LQRAQMEIDDVVGR+NR+LEESDL HLPYL
Sbjct: 305 LMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYL 364

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGTMLFVNVWAIQNDPTVWVEP  FN
Sbjct: 365 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFN 424

Query: 421 PDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEGG 480
           P+RF  +GEGFKWMPFGAGRRRCPGEGLGLRVVGL VGSLIQCF+WES+ GEC+DM EGG
Sbjct: 425 PERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGG 484

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 508
           GLTLPKALPL  LCRPRSN  HLL QI
Sbjct: 485 GLTLPKALPLHALCRPRSNVAHLLPQI 508

[Close]

BLAST of CSPI04G24590.2 vs. NCBI nr
Match: KAG7024285.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 861.3 bits (2224), Expect = 4.2e-246
Identity = 431/509 (84.68%), Postives = 452/509 (88.80%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+ N           DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSNK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWVEP KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFN 420

Query: 421 PDRFRGDGEG--FKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSE 480
           P RF  +GEG  FKWMPFGAGRRRCPGEGLGLRVVGL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 508
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of CSPI04G24590.2 vs. NCBI nr
Match: XP_023535659.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.0 bits (2218), Expect = 2.1e-245
Identity = 428/509 (84.09%), Postives = 453/509 (89.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAITFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRR+SSLEI S+HRLQTLSSIRVDEVRTLIRRLYN ENDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRVSSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTENDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+           + DAKKTMIEVLLSLQQKEP+YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS----------KKDAKKTMIEVLLSLQQKEPRYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ+EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQLEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFRGDGEG--FKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSE 480
           P RF  +GEG  FKWMPFGAGRRRCPGEGLGLRVVGL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 508
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of CSPI04G24590.2 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 444.1 bits (1141), Expect = 1.5e-124
Identity = 230/491 (46.84%), Postives = 320/491 (65.17%), Query Frame = 0


Query: 22  LHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHG--PIVFFQFGFRSVLVVSSP 81
           + R  NLPPSP  +LP+IG L LLK PLHR    +S   G  PI+  + G R V VVSS 
Sbjct: 24  IKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSH 83

Query: 82  SAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQ 141
           S AE+C +KNDV+ ANR   ++ K++ Y  T+++ A YG+HWRNLRRI ++EI S+HRL 
Sbjct: 84  SLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLN 143

Query: 142 TLSSIRVDEVRTLI---RRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAYS 201
           + SSIR DE+  LI    R  + E   V+M++ F  + FN  +RM+AGK YYG D A   
Sbjct: 144 SFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYG-DGAEDD 203

Query: 202 EEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEEH 261
            EA R RE+  E     G  N  D+L ++ W+    G E R++++  + D+++Q L++E 
Sbjct: 204 PEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIKKIASRLDEFLQGLVDER 263

Query: 262 RKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVLLLAGTEGSINT 321
           R+            +   + TM++ LL LQ+ +P+YY D  I+G+ML L+LAGT+ S  T
Sbjct: 264 REG-----------KEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAVT 323

Query: 322 MEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIHETLRMYPPGPL 381
           +EW LS LLNHP  L +A+ EID+ VG  NRL+EESDL+HLPYL++++ E+LR+YP  PL
Sbjct: 324 LEWTLSALLNHPQILSKARDEIDNKVG-LNRLVEESDLSHLPYLQNIVSESLRLYPASPL 383

Query: 382 LIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRFRGDGEGFKWMPF 441
           L+PH +SEDC VGG+H+P GTML  N WAI  DP +W +P  F P+RF  +GE  K + F
Sbjct: 384 LVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEGEAQKLLGF 443

Query: 442 GAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSE-GGGLTLPKALPLRTLCR 501
           G GRR CPG GL  R+  L +GSLIQCFEWE +  E +DM+E GGG+ +PKA+PL  +C+
Sbjct: 444 GLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMCK 498

Query: 502 PRSNATHLLSQ 507
            R     +L++
Sbjct: 504 ARPVVGKILNE 498

[Close]

BLAST of CSPI04G24590.2 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 440.3 bits (1131), Expect = 2.2e-123
Identity = 234/507 (46.15%), Postives = 325/507 (64.10%), Query Frame = 0


Query: 3   IPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHG- 62
           I  L  L LF L IF      R  NLPPSP +SLP+IG L+LLK PLHRT   +S+  G 
Sbjct: 6   ILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGN 65

Query: 63  -PIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 122
            P+   + G R V V+SS S AE+C +KNDV+ ANRP+    K+LGYN T LL A YG+H
Sbjct: 66  APVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDH 125

Query: 123 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFNI 182
           WRNLRRI+++EI S+HRL +   IR DE+R LI  L          V+M+T    +  N 
Sbjct: 126 WRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNT 185

Query: 183 TMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENR 242
           T+RM+AGKRY+GED    +++A   + +  E    +G  N  D+L +++WV      E R
Sbjct: 186 TIRMLAGKRYFGED----NDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYEKR 245

Query: 243 LRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEY 302
           ++ L  + D ++Q L++E R             + +  +TMI+ LL+LQ  +P YY D  
Sbjct: 246 IKNLGNRFDTFLQKLVDEKR------------AEKEKGETMIDHLLALQDIQPDYYTDVI 305

Query: 303 IRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHL 362
           I+G++L L++AGT+ S  T+EW +S LLNHP  L++A+MEID+ VG  +RL++ESD+ +L
Sbjct: 306 IKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVG-LDRLVDESDIVNL 365

Query: 363 PYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPR 422
            YL+S++ ETLRMYP  PLL+PH SSEDC VGG+ +P+GTM+  N WA+  DP VW +P 
Sbjct: 366 SYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPE 425

Query: 423 KFNPDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMS 482
            F P+RF  +GE  K + FG GRR CPG GL  R++   +GSL+QCFEWE +  + +DM+
Sbjct: 426 IFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMT 485

Query: 483 EGGGLTLPKALPLRTLCRPRSNATHLL 505
           E  G TLPKA+PLR +C+ RS    L+
Sbjct: 486 EDKGATLPKAIPLRAMCKARSIVDKLI 492

[Close]

BLAST of CSPI04G24590.2 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 439.5 bits (1129), Expect = 3.7e-123
Identity = 230/504 (45.63%), Postives = 327/504 (64.88%), Query Frame = 0


Query: 8   LLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISR--RHGPIVF 67
           +L + L  I+    L R  NLPPSP  SLP+IG L LLK P+HRT   +S+   + PI  
Sbjct: 10  ILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFS 69

Query: 68  FQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 127
            + G R V V SS S AE+C +KNDV+ ANRP  +  K++ Y+YT+++ A YG+HWRNLR
Sbjct: 70  LRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLR 129

Query: 128 RISSLEILSSHRLQTLSSIRVDEVRTLIRRL---YNAENDVVDMRTEFFEVMFNITMRMI 187
           RI S+EI S+HRL +  SIR DE+R L+ RL   ++ E   VDM++   ++ FN  +RM+
Sbjct: 130 RIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMV 189

Query: 188 AGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQ 247
           AGKRYYG+ V     EA R R++  +    +G  N  D+L V++   L    E R+++L 
Sbjct: 190 AGKRYYGDGVED-DPEAKRVRQLIADVVACAGAGNAVDYLPVLR---LVSDYETRVKKLA 249

Query: 248 IKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLM 307
            + D+++Q L++E R+                  TMI+ LL+LQ+ +P Y+ D  I+G M
Sbjct: 250 GRLDEFLQGLVDEKREAKEKG------------NTMIDHLLTLQESQPDYFTDRIIKGNM 309

Query: 308 LVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRS 367
           L L+LAGT+ S  T+EW LS +LNHP  L +A+ EID  +G  +RL++ESD+++LPYL++
Sbjct: 310 LALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIG-LDRLMDESDISNLPYLQN 369

Query: 368 LIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPD 427
           ++ ETLR+YP  P+L+PH +SEDC V G+ +P GT+L  NVWAI  DP +W +P  F P+
Sbjct: 370 IVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPE 429

Query: 428 RFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEGGGL 487
           RF  +GE  K MPFG GRR CPG GL  R++ L +GSLIQC EWE + GE +DMSEG G+
Sbjct: 430 RFEKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEVDMSEGKGV 489

Query: 488 TLPKALPLRTLCRPRSNATHLLSQ 507
           T+PKA PL  +CR R +   + ++
Sbjct: 490 TMPKAKPLEAMCRARPSVVKIFNE 495

[Close]

BLAST of CSPI04G24590.2 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 436.4 bits (1121), Expect = 3.1e-122
Identity = 222/490 (45.31%), Postives = 319/490 (65.10%), Query Frame = 0


Query: 26  RNLPPSPFLSLPIIGQLYLLKTP-LHRTLSEISRRHGPIVFFQFGFRSVLVVSSPSAAED 85
           +NLPPSP +  PIIG L+LLK P LHRTLS +S   GP+   + G R  +++SSP+AAE+
Sbjct: 28  KNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPVFSLRLGSRLAVIISSPTAAEE 87

Query: 86  C-LSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSS 145
           C L+KND++ ANRPR + GKY+ Y+YTS++ APYG+HWRNLRRI++LE+ S++RL   + 
Sbjct: 88  CFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRNLRRITALEVFSTNRLNASAE 147

Query: 146 IRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAYSEEAG 205
           IR DEV+ L+++L++   +    V++R     +  N+ MRM+ GKR++ ED    +  + 
Sbjct: 148 IRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIMRMMTGKRFFEEDDGGKAGISL 207

Query: 206 RFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEEHRKKM 265
            FRE+  E   LS   N  DFL  ++W    KG+  R + +  + D  +Q  ++EHR   
Sbjct: 208 EFRELVAEILELSAADNPADFLPALRWFDY-KGLVKRAKRIGERMDSLLQGFLDEHR--- 267

Query: 266 NNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWL 325
                 ++  + + K TMI  LL  Q+KEP  Y D+ I+GL+L++++ GT+ S  T+EW 
Sbjct: 268 ------ANKDRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLILMMVVGGTDTSALTVEWA 327

Query: 326 LSLLLNHPHSLQRAQMEIDDVV--GRTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLI 385
           +S LLNHP  L+  +  ID  +    + RLL+E DL ++ YL++++ ETLR+YP  PL++
Sbjct: 328 MSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKNVVSETLRLYPVAPLMV 387

Query: 386 PHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRFRGDGE----GFKWM 445
           PH  S DC +GGF+VP  T++ VN+WAI  DP+VW +P  F P+RF G  +      K M
Sbjct: 388 PHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFEGSDQFGHYNGKMM 447

Query: 446 PFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECIDMSEGGGLTLPKALPLRTLC 505
           PFG GRR CPG  L  RVVGL++GS+IQCFEWES  G  +DM+EG GL+LPKA PL   C
Sbjct: 448 PFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDMTEGPGLSLPKAEPLVVTC 507

[Close]

BLAST of CSPI04G24590.2 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 434.1 bits (1115), Expect = 1.5e-121
Identity = 229/503 (45.53%), Postives = 326/503 (64.81%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           +++ FL L I   L +  +   +R  NLPPSP   LP+IG L+LLK P+HRT   IS+  
Sbjct: 7   LLLSFLFLTISIKLLLTKS---NRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSL 66

Query: 61  G--PIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYG 120
           G  PI   + G R V V+SS S AE+C +KNDV+ ANRP ++  K++GYN+T+++ A YG
Sbjct: 67  GNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYG 126

Query: 121 EHWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMF 180
           +HWRNLRRI+++EI SSHR+ T SSIR DE+R LI  L          V++++    + F
Sbjct: 127 DHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAF 186

Query: 181 NITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 240
           N  + M+AGKRYYG      ++EA   RE+  E    +G  NL D+L  + WV      E
Sbjct: 187 NNIIMMVAGKRYYGTGTED-NDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFE 246

Query: 241 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 300
           N+ + L  + D  +Q L++E R             + +  +T+I+ LLS Q+ EP+YY D
Sbjct: 247 NQTKILGNRLDRVLQKLVDEKR------------AEKEKGQTLIDHLLSFQETEPEYYTD 306

Query: 301 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLT 360
             I+G++L L+LAGT+ S  T+EW +S LLNHP  L++A+ EIDD +G ++RL+EESD+ 
Sbjct: 307 VIIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIG-SDRLVEESDIV 366

Query: 361 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 420
           +L YL++++ ETLR+YP  PLL+PH SS++C V G+ +P  T+L  NVWA+  DP +W E
Sbjct: 367 NLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEE 426

Query: 421 PRKFNPDRFRGDGEGFKWMPFGAGRRRCPGEGLGLRVVGLVVGSLIQCFEWESMDGECID 480
           P +F P+RF  +GE  K MPFG GRR CPG  LG R+V L +G LIQ FEWE +  E +D
Sbjct: 427 PERFKPERFEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVD 486

Query: 481 MSEGGGLTLPKALPLRTLCRPRS 499
           M+EG G+T+PKA PLR +C+ R+
Sbjct: 487 MTEGEGITMPKATPLRAMCKARA 489

The following BLAST results are available for this feature:

BLAST of CSPI04G24590.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	1.3e-133	48.53	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	1.4e-122	45.05	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ8	5.7e-121	46.22	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	2.8e-120	46.20	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q0WTF4	3.1e-119	45.89	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]

BLAST of CSPI04G24590.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0F1	4.5e-294	99.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1	[more]
A0A1S3BVF3	3.9e-274	93.31	cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1	[more]
A0A6J1FED9	2.2e-245	84.28	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=...	[more]
A0A6J1INS3	1.9e-244	83.89	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 ...	[more]
A0A7J0EHR3	4.6e-182	63.33	Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g000763...	[more]

BLAST of CSPI04G24590.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141396.1	9.2e-294	99.61	cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [C...	[more]
XP_008452618.1	8.1e-274	93.31	PREDICTED: cytochrome P450 81E8-like [Cucumis melo]	[more]
XP_038899302.1	3.2e-262	88.36	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
KAG7024285.1	4.2e-246	84.68	Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023535659.1	2.1e-245	84.09	isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI04G24590.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37340.1	1.5e-124	46.84	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37330.1	2.2e-123	46.15	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37370.1	3.7e-123	45.63	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37310.1	3.1e-122	45.31	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT4G37320.1	1.5e-121	45.53	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 81..102 score: 25.45 coord: 361..379 score: 34.78 coord: 297..314 score: 26.01 coord: 317..343 score: 36.21 coord: 402..426 score: 35.4 coord: 443..466 score: 34.66 coord: 57..76 score: 28.43 coord: 433..443 score: 57.48 coord: 173..191 score: 24.18
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 434..443 score: 60.07 coord: 362..373 score: 40.54 coord: 308..325 score: 31.08 coord: 443..454 score: 31.81
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 30..485 e-value: 9.2E-88 score: 295.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..505 e-value: 2.7E-118 score: 397.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..497
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 12..472 e-value: 1.3E-15 score: 55.1
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 13..506
None	No IPR available	PANTHER	PTHR47947:SF24	CYTOCHROME P450 81E8	coord: 13..506
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 436..445

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G24590	CSPI04G24590	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G24590.2.utr5p1	CSPI04G24590.2.utr5p1	five_prime_UTR
CSPI04G24590.2.utr5p2	CSPI04G24590.2.utr5p2	five_prime_UTR
CSPI04G24590.2.utr5p3	CSPI04G24590.2.utr5p3	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G24590.2.cds3	CSPI04G24590.2.cds3	CDS
CSPI04G24590.2.cds4	CSPI04G24590.2.cds4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G24590.2.utr3p1	CSPI04G24590.2.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G24590.2	CSPI04G24590.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen