CSPI04G23120.1 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI04G23120.1
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING/U-box superfamily protein
LocationChr4: 21296222 .. 21300067 (-)
Sequence length1946
RNA-Seq ExpressionCSPI04G23120.1
SyntenyCSPI04G23120.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAAATGAAGTGATAAATTACAATTCTTTTTTTGGGCATAAGAAAAACAGTTGGCTGAGTCCAATCAGAAATCTAATTCTGGAAGAGAAAATGGGAAATGGAGAGGGACGTTTTGATGCTATTGATTGACTAAATTCACAAATACCCATCTACAACTTCTCAACAACACACTCTCTCTCGCCGATCATACCCCCCATTTACTGTGAGTTCCTTCTCCCATTTCGCTCTTTTTCTCTTGTTTTACCGATTCTATCTTTGCTTTTTCCCCTTTTGTTGCGCTTAGTTACGATTTTCGTTATCTTTGAGGTCAATTTATTGCATACCCTTTCTTTGATTTCTTGGGAACTGTTGGAGTTTGGTGCTGATTTCTTCCAAATTGATGAATATGCCAATCTTAGTGGCGTATGATTGTGAGCATTGGGTATTTTCCCTCGGTCTTGTTTTCTTTCCCTTGTTTCTTTATGGGTTTTAATTGATGGGGGCAAGGAGCAAGATTGTTTAACTGAAAGCCTTATTCGTGGGATTTATGAAAACGTTGATGGGTATGGATGGAATGTTGTTGGCTTTGCATTTTAGTATGCATTGTACATTTTTTAACTAAGATGTTGTTAATGTTAAGTTGTTTGTGAGTTTTGCGTGGACTTCGAGTAAAAGCTGTTTAATAAAATTCATTTTCTGTACCACCTGCCTACTCATATAAATTTAGAATTTGGTGAAATTTATAATAGCATTTTTATATTGATTGTGTGTATTCTCTCATAAAACCTCCCAATTGTATATATTTTTTTTCCTGTCCTTCAAAATTTCACCACCCAATATTTTCTGTATATTGATCCTAGGCATTGAAGATAAGGATAAGGGTAATTTTATTGGAACATCTTGAGACGAAAAGAATGGGGATTGTTGGCAGTTTTGTGGATAAATCTTAGGAATGATGTTGGATGTTGAAAAGTCAAAAACCTGCTGATTATCTCCCATGGATTTAGAAAATGGGTAAAGTTTTTGTGGTTGGAGTAATGAAAACACTCCCAAAGTAATGTAAATACACGATATCCTAGAGTACTGGTGGTTAATAGATGCATCAACGTTTTCTTTAGGTGCTTGCTCATGTTACCTTTTCCCCACTACTTTTCTTTACAATGACGTGCTGATCTTTACCTCCTTATTAGTGCTTGAGAAGCTTGTGCCAATGTGCAATTTTTCCATGATGTATAATTCATATTCCTTGGCATTAGTAAAATTTCTACTTGTTTAAGCATCTGTTATGCTTGCAAGGTTTTTATCTTTTCTCTCATATGGAAAGGTAAATTTGCTTATTTTCAGTACTTTTATTCTTGAGATGGTTCCCTTTGTCACATTCAATCTCCCTTCATTTTAATAAAATGAGTTCATCGAGTGAATTTTCTTATCTGACAAGTTTGATGTTGCTTGTTGATATCATTCAACAGAATACTAATGTTAAACATATTTTTGGTTTTGAGAGTAGGTATAACTGCACTTTCATGGACATAAGGGAAAGCACTCGTGTCCCTTGTCATACACCTCCAGCAATGGATATGGAATTAGATCAACCAAGTCTTCTTCCAGAGCTTTCTTTTTCTAGCCAGGGAGCCTTCTCACGGTGGAGTAGCCATTCGATGGTAACAGCTTCCAGAAACTCAATGAATCCTGATGTTCAACATCTACCTGAGCATGCTAATGGCGCCATACTCTATGGAGTGAGCCAACACAATGGTTCTAGGGTTCAGTGTGCTCAAGATTTACAGGTCACAACTGCTGCCAATCCCTGTTCTTATCTGACCTCACCATATGGTAATTACAATCAGCATTTCCCATCTCCTAGAAATGGATTGATTGGAAATCCTGCAGATACATATGCCAGGAATAGTCATTTTATTGAAGGTGGATTTCCGTACAAGAGAAGATTTTCTGAAGGGTTCCCTGTGAGTTTCCAGGGTCCCAATAGTTCTGCAAGCTTCGAGTCTTTAAATGCACCTTTTAATTCAATGCATGGACATGCTCGTAGCCATTTCATTAGAGGAGACGTAATGGTTCAGCACCAACAGCCAGCTAACAATGCTCTTTGGTTAGACCACCCTGTAAATTTGAACTTTGAAGATCGAAACACTTGGTCCTGGAACCTAGCTCCTGGTGGTTTCCCTATTCATGGTATATTTTTCTTCTTCATTCTATTAAAAAGGATCGATTGTTCATCTATTTATTGCATTTTTTGTGTTTTTCTTCAATTTTTGTTTATAACTATTTCTCAGGACGAAATTGGTTGCATAGTTACATGGAAGATTTTTCTTCAGATATGCTACCCTTTCTTCCATTTAGGTTGCTGGAACTTTAGAAGTTTTTCTTTTCAAACTACTTTCATGCTAGGAGATGCTGTGTTAAAGTATAATATTGAGTTCTACTGTCAATTATAGTTTAAACTACGTTGGTGTTTATTGCAATTCACGACTTGACAGGGGACAGTGGGATTAGAGGCTTTTCAGAAAGTTTTAATTCAGGAATTCATAGTTTTAGTGAAATGTCTGGGATGAGTTCTCCAAATTTTCAGCATCCTCCTTCCACAATAATCCAGCATTCGAGTCATCGAGTTCCACTACCACAGGCACACTTGCAAGGACAAAGAGGCCAAAATATTAGTTTACATCCCCAAGCAGCAGCTACTACACCAAGGGTTCCTTTGAGTTCTGCTTATGGGATCATGCACCAGTATTCAGAGCTAGAGCCTAGGCATACAAGAGATCTTGCATTTAGTGACCACATAACATATAATCTTCATCAAAGCAGTGTTGATCCAGAGACAATGTTTCGACGCCGTTCAACATATCAACTGAGAGTTCCAGAGGAGGATGTAACTATTTTTTCTATATTTTTGCCTCTGTTATACAAATTTATATTTCTGCATTATTTTATTAATTCATGCTGATATCATATTGATGAATTTGATGGGATGAGATGAGATGAAGTTGGTATATGCAGGAATTCTCTGTACGTGGGGCTCGGGTGTCAGCTTCAGCTTCAGACTCTAATGATATAGGAGGTTTTGCTGACACATACGGAGATATGCGCTTGGATATTGAAAACATGTCATATGAGGTGAAGTAATTCTCATTTTCAGGTTTGAAGAACTTTGATTTTACAATATAAAAATATGCTTAATACCAATGTATAGGAGCTTCTTGAATTAGAGGAACGGATTGGCTATGTCGGAACTGGGTTGTCTGAGAAAATAATTACGAGTCTACTGAAGACAAGGATTTCTGTACCATCTGCAAGGGATGTAAATTTAGAAGTCGGCGCAACCTCTATGAATGAGGAAACAAATTCATGCACCATATGCCTGGTAACTTCATCAATATACTTCCTGCTTGTGTTTAGAAAAGAAGTTATTTGGACTATATGTAACTGATAGCGATTATCCTTTAAAATGTGGCCTGAATGGATTTATAGGATGTCATCGACGACGGGACAAAGATTGGAATCCTTGATTGCAAACATTATTATCATGCAGATTGCTTAAAACAATGGCTGCTCATAAAAAACGTTTGCCCAGTCTGCAAATCGGAGGCATTGAAAAGATGAATGGATTGGGTTAATTTTGAACAGTTGAGTTACGGCTTAGCTGTTCATCGACGATCAAACAATGATTGGTTTAAACTATATCGCACGTTTGTAAATGTTTTGCACATATATACAACAATAAGACTCCAACTCGTTTTTATTTTGTTGGTTTCTTAAACTGTGAATGCATTTTTGTACATAGTTGTGTTTATATTGTGGAACTGTACATGTGAAATGTTAAGACTTTCTGTCTATTCAGTATTTTGTTA

mRNA sequence

GTTAAATGAAGTGATAAATTACAATTCTTTTTTTGGGCATAAGAAAAACAGTTGGCTGAGTCCAATCAGAAATCTAATTCTGGAAGAGAAAATGGGAAATGGAGAGGGACGTTTTGATGCTATTGATTGACTAAATTCACAAATACCCATCTACAACTTCTCAACAACACACTCTCTCTCGCCGATCATACCCCCCATTTACTGTATAACTGCACTTTCATGGACATAAGGGAAAGCACTCGTGTCCCTTGTCATACACCTCCAGCAATGGATATGGAATTAGATCAACCAAGTCTTCTTCCAGAGCTTTCTTTTTCTAGCCAGGGAGCCTTCTCACGGTGGAGTAGCCATTCGATGGTAACAGCTTCCAGAAACTCAATGAATCCTGATGTTCAACATCTACCTGAGCATGCTAATGGCGCCATACTCTATGGAGTGAGCCAACACAATGGTTCTAGGGTTCAGTGTGCTCAAGATTTACAGGTCACAACTGCTGCCAATCCCTGTTCTTATCTGACCTCACCATATGGTAATTACAATCAGCATTTCCCATCTCCTAGAAATGGATTGATTGGAAATCCTGCAGATACATATGCCAGGAATAGTCATTTTATTGAAGGTGGATTTCCGTACAAGAGAAGATTTTCTGAAGGGTTCCCTGTGAGTTTCCAGGGTCCCAATAGTTCTGCAAGCTTCGAGTCTTTAAATGCACCTTTTAATTCAATGCATGGACATGCTCGTAGCCATTTCATTAGAGGAGACGTAATGGTTCAGCACCAACAGCCAGCTAACAATGCTCTTTGGTTAGACCACCCTGTAAATTTGAACTTTGAAGATCGAAACACTTGGTCCTGGAACCTAGCTCCTGGTGGTTTCCCTATTCATGGGGACAGTGGGATTAGAGGCTTTTCAGAAAGTTTTAATTCAGGAATTCATAGTTTTAGTGAAATGTCTGGGATGAGTTCTCCAAATTTTCAGCATCCTCCTTCCACAATAATCCAGCATTCGAGTCATCGAGTTCCACTACCACAGGCACACTTGCAAGGACAAAGAGGCCAAAATATTAGTTTACATCCCCAAGCAGCAGCTACTACACCAAGGGTTCCTTTGAGTTCTGCTTATGGGATCATGCACCAGTATTCAGAGCTAGAGCCTAGGCATACAAGAGATCTTGCATTTAGTGACCACATAACATATAATCTTCATCAAAGCAGTGTTGATCCAGAGACAATGTTTCGACGCCGTTCAACATATCAACTGAGAGTTCCAGAGGAGGATGAATTCTCTGTACGTGGGGCTCGGGTGTCAGCTTCAGCTTCAGACTCTAATGATATAGGAGGTTTTGCTGACACATACGGAGATATGCGCTTGGATATTGAAAACATGTCATATGAGGAGCTTCTTGAATTAGAGGAACGGATTGGCTATGTCGGAACTGGGTTGTCTGAGAAAATAATTACGAGTCTACTGAAGACAAGGATTTCTGTACCATCTGCAAGGGATGTAAATTTAGAAGTCGGCGCAACCTCTATGAATGAGGAAACAAATTCATGCACCATATGCCTGGATGTCATCGACGACGGGACAAAGATTGGAATCCTTGATTGCAAACATTATTATCATGCAGATTGCTTAAAACAATGGCTGCTCATAAAAAACGTTTGCCCAGTCTGCAAATCGGAGGCATTGAAAAGATGAATGGATTGGGTTAATTTTGAACAGTTGAGTTACGGCTTAGCTGTTCATCGACGATCAAACAATGATTGGTTTAAACTATATCGCACGTTTGTAAATGTTTTGCACATATATACAACAATAAGACTCCAACTCGTTTTTATTTTGTTGGTTTCTTAAACTGTGAATGCATTTTTGTACATAGTTGTGTTTATATTGTGGAACTGTACATGTGAAATGTTAAGACTTTCTGTCTATTCAGTATTTTGTTA

Coding sequence (CDS)

ATGGACATAAGGGAAAGCACTCGTGTCCCTTGTCATACACCTCCAGCAATGGATATGGAATTAGATCAACCAAGTCTTCTTCCAGAGCTTTCTTTTTCTAGCCAGGGAGCCTTCTCACGGTGGAGTAGCCATTCGATGGTAACAGCTTCCAGAAACTCAATGAATCCTGATGTTCAACATCTACCTGAGCATGCTAATGGCGCCATACTCTATGGAGTGAGCCAACACAATGGTTCTAGGGTTCAGTGTGCTCAAGATTTACAGGTCACAACTGCTGCCAATCCCTGTTCTTATCTGACCTCACCATATGGTAATTACAATCAGCATTTCCCATCTCCTAGAAATGGATTGATTGGAAATCCTGCAGATACATATGCCAGGAATAGTCATTTTATTGAAGGTGGATTTCCGTACAAGAGAAGATTTTCTGAAGGGTTCCCTGTGAGTTTCCAGGGTCCCAATAGTTCTGCAAGCTTCGAGTCTTTAAATGCACCTTTTAATTCAATGCATGGACATGCTCGTAGCCATTTCATTAGAGGAGACGTAATGGTTCAGCACCAACAGCCAGCTAACAATGCTCTTTGGTTAGACCACCCTGTAAATTTGAACTTTGAAGATCGAAACACTTGGTCCTGGAACCTAGCTCCTGGTGGTTTCCCTATTCATGGGGACAGTGGGATTAGAGGCTTTTCAGAAAGTTTTAATTCAGGAATTCATAGTTTTAGTGAAATGTCTGGGATGAGTTCTCCAAATTTTCAGCATCCTCCTTCCACAATAATCCAGCATTCGAGTCATCGAGTTCCACTACCACAGGCACACTTGCAAGGACAAAGAGGCCAAAATATTAGTTTACATCCCCAAGCAGCAGCTACTACACCAAGGGTTCCTTTGAGTTCTGCTTATGGGATCATGCACCAGTATTCAGAGCTAGAGCCTAGGCATACAAGAGATCTTGCATTTAGTGACCACATAACATATAATCTTCATCAAAGCAGTGTTGATCCAGAGACAATGTTTCGACGCCGTTCAACATATCAACTGAGAGTTCCAGAGGAGGATGAATTCTCTGTACGTGGGGCTCGGGTGTCAGCTTCAGCTTCAGACTCTAATGATATAGGAGGTTTTGCTGACACATACGGAGATATGCGCTTGGATATTGAAAACATGTCATATGAGGAGCTTCTTGAATTAGAGGAACGGATTGGCTATGTCGGAACTGGGTTGTCTGAGAAAATAATTACGAGTCTACTGAAGACAAGGATTTCTGTACCATCTGCAAGGGATGTAAATTTAGAAGTCGGCGCAACCTCTATGAATGAGGAAACAAATTCATGCACCATATGCCTGGATGTCATCGACGACGGGACAAAGATTGGAATCCTTGATTGCAAACATTATTATCATGCAGATTGCTTAAAACAATGGCTGCTCATAAAAAACGTTTGCCCAGTCTGCAAATCGGAGGCATTGAAAAGATGA

Protein sequence

MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQHLPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGNPADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRGDVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHSFSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSAYGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGARVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEALKR*
Homology
BLAST of CSPI04G23120.1 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.4e-25
Identity = 56/120 (46.67%), Postives = 80/120 (66.67%), Query Frame = 0

Query: 371 DIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVN 430
           ++    D + DMRLDI++M+YEELL LEE+IG V TGL++  I   LKT + VP +  ++
Sbjct: 399 EVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSLFVPGSSCMS 458

Query: 431 LEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEAL 490
            +   +SM  E ++C IC +       IG LDC H YH DC+KQWL++KN+CP+CK+ AL
Sbjct: 459 NKSSESSM--ENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLCPICKTTAL 516

BLAST of CSPI04G23120.1 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 2.2e-24
Identity = 57/115 (49.57%), Postives = 71/115 (61.74%), Query Frame = 0

Query: 377 DTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGAT 436
           D + DMRLDI+NMSYEELL LEERIG V TGLSE+ +T LLK R                
Sbjct: 561 DRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQR---------KFSSWRL 620

Query: 437 SMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEALK 492
             + E   C IC +   DG  +G LDC H +H  C++QWL++KN CP+CK+ ALK
Sbjct: 621 EASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTALK 666

BLAST of CSPI04G23120.1 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.4e-23
Identity = 51/115 (44.35%), Postives = 74/115 (64.35%), Query Frame = 0

Query: 377 DTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGAT 436
           D + +MRLD++NMSYEELL L ERIG V TGLSE++I   +K        +  +    + 
Sbjct: 594 DRHREMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQH------KHTSSSPSSV 653

Query: 437 SMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEALK 492
            +++    C IC +   +G  +G L C H +H DC+KQW++IKN+CP+CK+EALK
Sbjct: 654 ELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTEALK 702

BLAST of CSPI04G23120.1 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 7.7e-22
Identity = 50/114 (43.86%), Postives = 71/114 (62.28%), Query Frame = 0

Query: 377 DTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGAT 436
           D + DMRLD++NMSYEELL L ERIG V TGLSE++I  ++K      S         A 
Sbjct: 560 DRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSS---------AA 619

Query: 437 SMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEAL 491
             +++   C +C +   +G  +G L C H +H  C+KQWL++KN+CP+CK+ AL
Sbjct: 620 GSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVAL 664

BLAST of CSPI04G23120.1 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.1e-20
Identity = 49/114 (42.98%), Postives = 71/114 (62.28%), Query Frame = 0

Query: 377 DTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGAT 436
           D Y DMRLD++NMSYEELL LEERIG V TG++E+ I++ LK R    +          T
Sbjct: 579 DRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNRLKQRKYKSN----------T 638

Query: 437 SMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEAL 491
              ++   C +C +   +G  +G L+C H +H+ C+K+WL  KN+CP+CK+  L
Sbjct: 639 KSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNLCPICKTTGL 682

BLAST of CSPI04G23120.1 vs. ExPASy TrEMBL
Match: A0A0A0L238 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G628910 PE=4 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 5.0e-290
Identity = 491/492 (99.80%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH
Sbjct: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN
Sbjct: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HGHARSHFIRG
Sbjct: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGHARSHFIRG 180

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS
Sbjct: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA
Sbjct: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA
Sbjct: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR
Sbjct: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN
Sbjct: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEALKR
Sbjct: 481 VCPVCKSEALKR 492

BLAST of CSPI04G23120.1 vs. ExPASy TrEMBL
Match: A0A5D3DWX7 (Putative E3 ubiquitin-protein ligase HIP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001260 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.0e-266
Identity = 453/492 (92.07%), Postives = 465/492 (94.51%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRE TRVPCHTPPAMDMELDQPSLLPELS SSQGAFSRWSSHSMVTASRNSM PD QH
Sbjct: 1   MDIRERTRVPCHTPPAMDMELDQPSLLPELSLSSQGAFSRWSSHSMVTASRNSMTPDAQH 60

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQ +GSR+QCAQDL VTTAAN CSYLTSPYGN+NQH PSPRNGLIGN
Sbjct: 61  LPEHANGAILYGVSQRSGSRIQCAQDLHVTTAANSCSYLTSPYGNHNQHLPSPRNGLIGN 120

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HG+ARSH IRG
Sbjct: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGYARSHLIRG 180

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           D+MVQHQQPANNALWLDHPVN NFEDR+TW+WN AP GFPIHGDSGIRGFSESFNSGI S
Sbjct: 181 DIMVQHQQPANNALWLDHPVNFNFEDRSTWTWNQAPAGFPIHGDSGIRGFSESFNSGIQS 240

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQR QNISLHPQAAATTPRV LSSA
Sbjct: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRSQNISLHPQAAATTPRVTLSSA 300

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDL F+DHITYN HQS VDPETMFRR STYQLRVPEEDEFSVRGA
Sbjct: 301 YGIMHQYSELEPRHTRDLPFNDHITYNFHQSCVDPETMFRRPSTYQLRVPEEDEFSVRGA 360

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           R SASASDSNDIGGF DTY DMRLDIENMSYEELLELEERIGYVGTGLSE+II S LKT 
Sbjct: 361 RASASASDSNDIGGFNDTYRDMRLDIENMSYEELLELEERIGYVGTGLSEEIIMSQLKTS 420

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           IS+ SARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDC+HYYHADCLKQWLLIKN
Sbjct: 421 ISISSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCEHYYHADCLKQWLLIKN 480

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEAL R
Sbjct: 481 VCPVCKSEALTR 492

BLAST of CSPI04G23120.1 vs. ExPASy TrEMBL
Match: E5GBF9 (Zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.0e-266
Identity = 453/492 (92.07%), Postives = 465/492 (94.51%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRE TRVPCHTPPAMDMELDQPSLLPELS SSQGAFSRWSSHSMVTASRNSM PD QH
Sbjct: 1   MDIRERTRVPCHTPPAMDMELDQPSLLPELSLSSQGAFSRWSSHSMVTASRNSMTPDAQH 60

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQ +GSR+QCAQDL VTTAAN CSYLTSPYGN+NQH PSPRNGLIGN
Sbjct: 61  LPEHANGAILYGVSQRSGSRIQCAQDLHVTTAANSCSYLTSPYGNHNQHLPSPRNGLIGN 120

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HG+ARSH IRG
Sbjct: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGYARSHLIRG 180

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           D+MVQHQQPANNALWLDHPVN NFEDR+TW+WN AP GFPIHGDSGIRGFSESFNSGI S
Sbjct: 181 DIMVQHQQPANNALWLDHPVNFNFEDRSTWTWNQAPAGFPIHGDSGIRGFSESFNSGIQS 240

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQR QNISLHPQAAATTPRV LSSA
Sbjct: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRSQNISLHPQAAATTPRVTLSSA 300

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDL F+DHITYN HQS VDPETMFRR STYQLRVPEEDEFSVRGA
Sbjct: 301 YGIMHQYSELEPRHTRDLPFNDHITYNFHQSCVDPETMFRRPSTYQLRVPEEDEFSVRGA 360

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           R SASASDSNDIGGF DTY DMRLDIENMSYEELLELEERIGYVGTGLSE+II S LKT 
Sbjct: 361 RASASASDSNDIGGFNDTYRDMRLDIENMSYEELLELEERIGYVGTGLSEEIIMSQLKTS 420

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           IS+ SARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDC+HYYHADCLKQWLLIKN
Sbjct: 421 ISISSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCEHYYHADCLKQWLLIKN 480

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEAL R
Sbjct: 481 VCPVCKSEALTR 492

BLAST of CSPI04G23120.1 vs. ExPASy TrEMBL
Match: A0A1S3CKD0 (uncharacterized protein LOC103501794 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501794 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.0e-266
Identity = 453/492 (92.07%), Postives = 465/492 (94.51%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRE TRVPCHTPPAMDMELDQPSLLPELS SSQGAFSRWSSHSMVTASRNSM PD QH
Sbjct: 12  MDIRERTRVPCHTPPAMDMELDQPSLLPELSLSSQGAFSRWSSHSMVTASRNSMTPDAQH 71

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQ +GSR+QCAQDL VTTAAN CSYLTSPYGN+NQH PSPRNGLIGN
Sbjct: 72  LPEHANGAILYGVSQRSGSRIQCAQDLHVTTAANSCSYLTSPYGNHNQHLPSPRNGLIGN 131

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HG+ARSH IRG
Sbjct: 132 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGYARSHLIRG 191

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           D+MVQHQQPANNALWLDHPVN NFEDR+TW+WN AP GFPIHGDSGIRGFSESFNSGI S
Sbjct: 192 DIMVQHQQPANNALWLDHPVNFNFEDRSTWTWNQAPAGFPIHGDSGIRGFSESFNSGIQS 251

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQR QNISLHPQAAATTPRV LSSA
Sbjct: 252 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRSQNISLHPQAAATTPRVTLSSA 311

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDL F+DHITYN HQS VDPETMFRR STYQLRVPEEDEFSVRGA
Sbjct: 312 YGIMHQYSELEPRHTRDLPFNDHITYNFHQSCVDPETMFRRPSTYQLRVPEEDEFSVRGA 371

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           R SASASDSNDIGGF DTY DMRLDIENMSYEELLELEERIGYVGTGLSE+II S LKT 
Sbjct: 372 RASASASDSNDIGGFNDTYRDMRLDIENMSYEELLELEERIGYVGTGLSEEIIMSQLKTS 431

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           IS+ SARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDC+HYYHADCLKQWLLIKN
Sbjct: 432 ISISSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCEHYYHADCLKQWLLIKN 491

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEAL R
Sbjct: 492 VCPVCKSEALTR 503

BLAST of CSPI04G23120.1 vs. ExPASy TrEMBL
Match: A0A1S3CLG5 (uncharacterized protein LOC103501794 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501794 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.0e-266
Identity = 453/492 (92.07%), Postives = 465/492 (94.51%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRE TRVPCHTPPAMDMELDQPSLLPELS SSQGAFSRWSSHSMVTASRNSM PD QH
Sbjct: 1   MDIRERTRVPCHTPPAMDMELDQPSLLPELSLSSQGAFSRWSSHSMVTASRNSMTPDAQH 60

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQ +GSR+QCAQDL VTTAAN CSYLTSPYGN+NQH PSPRNGLIGN
Sbjct: 61  LPEHANGAILYGVSQRSGSRIQCAQDLHVTTAANSCSYLTSPYGNHNQHLPSPRNGLIGN 120

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HG+ARSH IRG
Sbjct: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGYARSHLIRG 180

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           D+MVQHQQPANNALWLDHPVN NFEDR+TW+WN AP GFPIHGDSGIRGFSESFNSGI S
Sbjct: 181 DIMVQHQQPANNALWLDHPVNFNFEDRSTWTWNQAPAGFPIHGDSGIRGFSESFNSGIQS 240

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQR QNISLHPQAAATTPRV LSSA
Sbjct: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRSQNISLHPQAAATTPRVTLSSA 300

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDL F+DHITYN HQS VDPETMFRR STYQLRVPEEDEFSVRGA
Sbjct: 301 YGIMHQYSELEPRHTRDLPFNDHITYNFHQSCVDPETMFRRPSTYQLRVPEEDEFSVRGA 360

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           R SASASDSNDIGGF DTY DMRLDIENMSYEELLELEERIGYVGTGLSE+II S LKT 
Sbjct: 361 RASASASDSNDIGGFNDTYRDMRLDIENMSYEELLELEERIGYVGTGLSEEIIMSQLKTS 420

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           IS+ SARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDC+HYYHADCLKQWLLIKN
Sbjct: 421 ISISSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCEHYYHADCLKQWLLIKN 480

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEAL R
Sbjct: 481 VCPVCKSEALTR 492

BLAST of CSPI04G23120.1 vs. NCBI nr
Match: XP_004146039.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X3 [Cucumis sativus] >XP_031740110.1 probable E3 ubiquitin-protein ligase ZFP1 isoform X3 [Cucumis sativus] >XP_031740111.1 probable E3 ubiquitin-protein ligase ZFP1 isoform X3 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.0e-289
Identity = 491/492 (99.80%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH
Sbjct: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN
Sbjct: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HGHARSHFIRG
Sbjct: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGHARSHFIRG 180

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS
Sbjct: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA
Sbjct: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA
Sbjct: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR
Sbjct: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN
Sbjct: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEALKR
Sbjct: 481 VCPVCKSEALKR 492

BLAST of CSPI04G23120.1 vs. NCBI nr
Match: KAE8649831.1 (hypothetical protein Csa_012901 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.0e-289
Identity = 491/492 (99.80%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH
Sbjct: 289 MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 348

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN
Sbjct: 349 LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 408

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HGHARSHFIRG
Sbjct: 409 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGHARSHFIRG 468

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS
Sbjct: 469 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 528

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA
Sbjct: 529 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 588

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA
Sbjct: 589 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 648

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR
Sbjct: 649 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 708

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN
Sbjct: 709 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 768

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEALKR
Sbjct: 769 VCPVCKSEALKR 780

BLAST of CSPI04G23120.1 vs. NCBI nr
Match: XP_031740109.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X1 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.0e-289
Identity = 491/492 (99.80%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH
Sbjct: 12  MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 71

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN
Sbjct: 72  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 131

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HGHARSHFIRG
Sbjct: 132 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGHARSHFIRG 191

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS
Sbjct: 192 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 251

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA
Sbjct: 252 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 311

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA
Sbjct: 312 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 371

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR
Sbjct: 372 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 431

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN
Sbjct: 432 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 491

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEALKR
Sbjct: 492 VCPVCKSEALKR 503

BLAST of CSPI04G23120.1 vs. NCBI nr
Match: XP_011654055.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X2 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.0e-289
Identity = 491/492 (99.80%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH
Sbjct: 12  MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 71

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN
Sbjct: 72  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 131

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HGHARSHFIRG
Sbjct: 132 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGHARSHFIRG 191

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS
Sbjct: 192 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 251

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA
Sbjct: 252 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 311

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA
Sbjct: 312 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 371

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR
Sbjct: 372 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 431

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN
Sbjct: 432 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 491

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEALKR
Sbjct: 492 VCPVCKSEALKR 503

BLAST of CSPI04G23120.1 vs. NCBI nr
Match: XP_008463711.1 (PREDICTED: uncharacterized protein LOC103501794 isoform X2 [Cucumis melo] >ADN33801.1 zinc finger protein [Cucumis melo subsp. melo] >TYK27938.1 putative E3 ubiquitin-protein ligase HIP1 [Cucumis melo var. makuwa])

HSP 1 Score: 928.7 bits (2399), Expect = 2.1e-266
Identity = 453/492 (92.07%), Postives = 465/492 (94.51%), Query Frame = 0

Query: 1   MDIRESTRVPCHTPPAMDMELDQPSLLPELSFSSQGAFSRWSSHSMVTASRNSMNPDVQH 60
           MDIRE TRVPCHTPPAMDMELDQPSLLPELS SSQGAFSRWSSHSMVTASRNSM PD QH
Sbjct: 1   MDIRERTRVPCHTPPAMDMELDQPSLLPELSLSSQGAFSRWSSHSMVTASRNSMTPDAQH 60

Query: 61  LPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPSPRNGLIGN 120
           LPEHANGAILYGVSQ +GSR+QCAQDL VTTAAN CSYLTSPYGN+NQH PSPRNGLIGN
Sbjct: 61  LPEHANGAILYGVSQRSGSRIQCAQDLHVTTAANSCSYLTSPYGNHNQHLPSPRNGLIGN 120

Query: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSMHGHARSHFIRG 180
           PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNS+HG+ARSH IRG
Sbjct: 121 PADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLNAPFNSIHGYARSHLIRG 180

Query: 181 DVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHS 240
           D+MVQHQQPANNALWLDHPVN NFEDR+TW+WN AP GFPIHGDSGIRGFSESFNSGI S
Sbjct: 181 DIMVQHQQPANNALWLDHPVNFNFEDRSTWTWNQAPAGFPIHGDSGIRGFSESFNSGIQS 240

Query: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSA 300
           FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQR QNISLHPQAAATTPRV LSSA
Sbjct: 241 FSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHLQGQRSQNISLHPQAAATTPRVTLSSA 300

Query: 301 YGIMHQYSELEPRHTRDLAFSDHITYNLHQSSVDPETMFRRRSTYQLRVPEEDEFSVRGA 360
           YGIMHQYSELEPRHTRDL F+DHITYN HQS VDPETMFRR STYQLRVPEEDEFSVRGA
Sbjct: 301 YGIMHQYSELEPRHTRDLPFNDHITYNFHQSCVDPETMFRRPSTYQLRVPEEDEFSVRGA 360

Query: 361 RVSASASDSNDIGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTR 420
           R SASASDSNDIGGF DTY DMRLDIENMSYEELLELEERIGYVGTGLSE+II S LKT 
Sbjct: 361 RASASASDSNDIGGFNDTYRDMRLDIENMSYEELLELEERIGYVGTGLSEEIIMSQLKTS 420

Query: 421 ISVPSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKN 480
           IS+ SARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDC+HYYHADCLKQWLLIKN
Sbjct: 421 ISISSARDVNLEVGATSMNEETNSCTICLDVIDDGTKIGILDCEHYYHADCLKQWLLIKN 480

Query: 481 VCPVCKSEALKR 493
           VCPVCKSEAL R
Sbjct: 481 VCPVCKSEALTR 492

BLAST of CSPI04G23120.1 vs. TAIR 10
Match: AT1G53190.1 (RING/U-box superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.8e-40
Identity = 153/468 (32.69%), Postives = 220/468 (47.01%), Query Frame = 0

Query: 53  SMNPDVQHLPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPS 112
           ++ P     P + N A+ YG+ Q++    Q       T    P     +P G   Q   S
Sbjct: 50  NLEPHSLQDPTYDNSAMFYGLPQYHHHPHQRVP----TNFYVPYVAFQAPPG---QLPSS 109

Query: 113 PRNGLIG-NPADTYARNSHFIE-GGFPYKRRFSEGFP----------VSFQGPNSSASF- 172
             +G++G +P   Y RN+HF++     YKR+ +EG P            F  P + A F 
Sbjct: 110 SSHGVVGVSPDHEYERNAHFMDHTRGTYKRKNAEGIPGQPQYLSTLAAPFNTPETIAPFG 169

Query: 173 ESLNAP-----FNSMHGHARSHFIRGDVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNL 232
            + N P        +  HA ++FI+G+    H  P   ++W D         R+  S + 
Sbjct: 170 GARNRPGAVTVNTVLPSHAPNNFIQGNYAGHHPFPPPGSMWYD-----QHHGRSDGSPSF 229

Query: 233 APGGFPIHGDSGIRGFSESFNSGIHSFSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHL 292
            P  + +HG +   G  ES +    SF        P+  +P      H  H  P P   +
Sbjct: 230 WPTPY-MHGSNIFAGSIESSSRNPTSF------MYPSQLNPRDHYYSHHHHPAPPP---V 289

Query: 293 QGQRGQNISLHPQAAATTP-RVPLSS--AYGIMHQ---YSELEPRHTRDLAFSDHITYNL 352
           QG RGQN +L+P  A++   RVP  S      M+     SE+   H   +  +    Y  
Sbjct: 290 QGMRGQNATLYPHTASSASYRVPPGSFTPQNTMNSGPLGSEMGSSHMGPVQPTGFRIYQH 349

Query: 353 HQ--SSVDPETMFRRR-STYQLRVPEEDEFSVRGARVSASASDSNDIGGFADTYGDMRLD 412
           HQ   SV   T+ + R    +LRV  +DE ++         S +N I    D + DMRLD
Sbjct: 350 HQRDDSVPVATLRQHRGGVPRLRVMPDDEVALLEFGDFLGGSGNNHI----DHHRDMRLD 409

Query: 413 IENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGATS---MNEET 472
           IE MSYEELL L ERIG V TGL E+ + + LKTR    +   +N E  ++S    + ET
Sbjct: 410 IEEMSYEELLALSERIGTVNTGLPEEDVKNHLKTR----TCSGINFEKESSSPRTKDLET 469

Query: 473 NSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEAL 491
             CTIC +   +  KI  LDC H YHA+CL++WL++KNVCP+CKSEAL
Sbjct: 470 EPCTICQESFKNEEKIATLDCGHEYHAECLEKWLIVKNVCPICKSEAL 487

BLAST of CSPI04G23120.1 vs. TAIR 10
Match: AT1G53190.2 (RING/U-box superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.8e-40
Identity = 153/468 (32.69%), Postives = 220/468 (47.01%), Query Frame = 0

Query: 53  SMNPDVQHLPEHANGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFPS 112
           ++ P     P + N A+ YG+ Q++    Q       T    P     +P G   Q   S
Sbjct: 50  NLEPHSLQDPTYDNSAMFYGLPQYHHHPHQRVP----TNFYVPYVAFQAPPG---QLPSS 109

Query: 113 PRNGLIG-NPADTYARNSHFIE-GGFPYKRRFSEGFP----------VSFQGPNSSASF- 172
             +G++G +P   Y RN+HF++     YKR+ +EG P            F  P + A F 
Sbjct: 110 SSHGVVGVSPDHEYERNAHFMDHTRGTYKRKNAEGIPGQPQYLSTLAAPFNTPETIAPFG 169

Query: 173 ESLNAP-----FNSMHGHARSHFIRGDVMVQHQQPANNALWLDHPVNLNFEDRNTWSWNL 232
            + N P        +  HA ++FI+G+    H  P   ++W D         R+  S + 
Sbjct: 170 GARNRPGAVTVNTVLPSHAPNNFIQGNYAGHHPFPPPGSMWYD-----QHHGRSDGSPSF 229

Query: 233 APGGFPIHGDSGIRGFSESFNSGIHSFSEMSGMSSPNFQHPPSTIIQHSSHRVPLPQAHL 292
            P  + +HG +   G  ES +    SF        P+  +P      H  H  P P   +
Sbjct: 230 WPTPY-MHGSNIFAGSIESSSRNPTSF------MYPSQLNPRDHYYSHHHHPAPPP---V 289

Query: 293 QGQRGQNISLHPQAAATTP-RVPLSS--AYGIMHQ---YSELEPRHTRDLAFSDHITYNL 352
           QG RGQN +L+P  A++   RVP  S      M+     SE+   H   +  +    Y  
Sbjct: 290 QGMRGQNATLYPHTASSASYRVPPGSFTPQNTMNSGPLGSEMGSSHMGPVQPTGFRIYQH 349

Query: 353 HQ--SSVDPETMFRRR-STYQLRVPEEDEFSVRGARVSASASDSNDIGGFADTYGDMRLD 412
           HQ   SV   T+ + R    +LRV  +DE ++         S +N I    D + DMRLD
Sbjct: 350 HQRDDSVPVATLRQHRGGVPRLRVMPDDEVALLEFGDFLGGSGNNHI----DHHRDMRLD 409

Query: 413 IENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGATS---MNEET 472
           IE MSYEELL L ERIG V TGL E+ + + LKTR    +   +N E  ++S    + ET
Sbjct: 410 IEEMSYEELLALSERIGTVNTGLPEEDVKNHLKTR----TCSGINFEKESSSPRTKDLET 469

Query: 473 NSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEAL 491
             CTIC +   +  KI  LDC H YHA+CL++WL++KNVCP+CKSEAL
Sbjct: 470 EPCTICQESFKNEEKIATLDCGHEYHAECLEKWLIVKNVCPICKSEAL 487

BLAST of CSPI04G23120.1 vs. TAIR 10
Match: AT3G15070.1 (RING/U-box superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 5.4e-39
Identity = 151/479 (31.52%), Postives = 212/479 (44.26%), Query Frame = 0

Query: 58  VQHLPEHA------NGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFP 117
           V HL  H+      N ++ YG  Q++    Q A +L    +  P  Y+  PY NY    P
Sbjct: 42  VPHLEAHSLQEPYDNNSMFYGPPQYHH---QHASNLGSGMSTAPNFYV--PYVNYEAP-P 101

Query: 118 S------PRNGLIGNPADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLN-- 177
           S        + ++G  +  + RN+HF++ GF  KR+ SE  P + Q P +  SF  LN  
Sbjct: 102 SFLLSHGSNDAVVGVTSTEHERNAHFMDHGF--KRKSSEVIPGNSQYPVAPCSFPQLNTS 161

Query: 178 --APFNSMH--------------------------GHARSHFIRGDVMVQHQQPANNALW 237
             APF+  H                           H  ++F +G+    H  P   ++W
Sbjct: 162 ETAPFSFPHFGTYPQPLDQRSVRNRAGAATMDPLLSHGHNNFSQGN-YAAHPFPPLGSIW 221

Query: 238 LDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHSFSEMSGMSSPNFQHP 297
            D   N N  D ++  W+ AP    +HG+      + S  SG   F        P +   
Sbjct: 222 YDQHCNGNRSDGSSSLWSQAPAVPYMHGNIA----TGSIESGNVCF--------PRYH-- 281

Query: 298 PSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSAYGIMHQYSE-LEPRH 357
                + SS R P P  +   QR   IS HP      P  P+   +     Y+E L P  
Sbjct: 282 -----ETSSSRNPTPSVY---QRNHYISHHP-----VPPPPIVYPHMPSASYAETLHPAS 341

Query: 358 TRDLAFSDHITYNLHQ---SSVDPETMFRRRSTYQLRVPEEDEFSVRGARVSASASDSND 417
              +       + ++Q       P  + R R     R    +E             D  +
Sbjct: 342 YSHMGQVQSTGFRINQYPGEDFVPAAILRHRELPHFRAMPANE------NAFWEVGDFYN 401

Query: 418 IGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNL 477
              + D + DMRLDIE+MSYEELL L ++IG V TGLS + +  LLK R S      +NL
Sbjct: 402 AVNYVDHHQDMRLDIEDMSYEELLALSDQIGTVKTGLSSEDVKELLKRRTST----RINL 461

Query: 478 EVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEAL 491
           E G  S + ET+SCTIC +   +  KI  LDC H YHA+CLK+WL+IKNVCP+CKSEAL
Sbjct: 462 EEG-PSTDLETDSCTICQENYKNEDKIATLDCMHKYHAECLKKWLVIKNVCPICKSEAL 473

BLAST of CSPI04G23120.1 vs. TAIR 10
Match: AT3G15070.2 (RING/U-box superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 5.4e-39
Identity = 151/479 (31.52%), Postives = 212/479 (44.26%), Query Frame = 0

Query: 58  VQHLPEHA------NGAILYGVSQHNGSRVQCAQDLQVTTAANPCSYLTSPYGNYNQHFP 117
           V HL  H+      N ++ YG  Q++    Q A +L    +  P  Y+  PY NY    P
Sbjct: 42  VPHLEAHSLQEPYDNNSMFYGPPQYHH---QHASNLGSGMSTAPNFYV--PYVNYEAP-P 101

Query: 118 S------PRNGLIGNPADTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSSASFESLN-- 177
           S        + ++G  +  + RN+HF++ GF  KR+ SE  P + Q P +  SF  LN  
Sbjct: 102 SFLLSHGSNDAVVGVTSTEHERNAHFMDHGF--KRKSSEVIPGNSQYPVAPCSFPQLNTS 161

Query: 178 --APFNSMH--------------------------GHARSHFIRGDVMVQHQQPANNALW 237
             APF+  H                           H  ++F +G+    H  P   ++W
Sbjct: 162 ETAPFSFPHFGTYPQPLDQRSVRNRAGAATMDPLLSHGHNNFSQGN-YAAHPFPPLGSIW 221

Query: 238 LDHPVNLNFEDRNTWSWNLAPGGFPIHGDSGIRGFSESFNSGIHSFSEMSGMSSPNFQHP 297
            D   N N  D ++  W+ AP    +HG+      + S  SG   F        P +   
Sbjct: 222 YDQHCNGNRSDGSSSLWSQAPAVPYMHGNIA----TGSIESGNVCF--------PRYH-- 281

Query: 298 PSTIIQHSSHRVPLPQAHLQGQRGQNISLHPQAAATTPRVPLSSAYGIMHQYSE-LEPRH 357
                + SS R P P  +   QR   IS HP      P  P+   +     Y+E L P  
Sbjct: 282 -----ETSSSRNPTPSVY---QRNHYISHHP-----VPPPPIVYPHMPSASYAETLHPAS 341

Query: 358 TRDLAFSDHITYNLHQ---SSVDPETMFRRRSTYQLRVPEEDEFSVRGARVSASASDSND 417
              +       + ++Q       P  + R R     R    +E             D  +
Sbjct: 342 YSHMGQVQSTGFRINQYPGEDFVPAAILRHRELPHFRAMPANE------NAFWEVGDFYN 401

Query: 418 IGGFADTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNL 477
              + D + DMRLDIE+MSYEELL L ++IG V TGLS + +  LLK R S      +NL
Sbjct: 402 AVNYVDHHQDMRLDIEDMSYEELLALSDQIGTVKTGLSSEDVKELLKRRTST----RINL 461

Query: 478 EVGATSMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEAL 491
           E G  S + ET+SCTIC +   +  KI  LDC H YHA+CLK+WL+IKNVCP+CKSEAL
Sbjct: 462 EEG-PSTDLETDSCTICQENYKNEDKIATLDCMHKYHAECLKKWLVIKNVCPICKSEAL 473

BLAST of CSPI04G23120.1 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-24
Identity = 51/115 (44.35%), Postives = 74/115 (64.35%), Query Frame = 0

Query: 377 DTYGDMRLDIENMSYEELLELEERIGYVGTGLSEKIITSLLKTRISVPSARDVNLEVGAT 436
           D + +MRLD++NMSYEELL L ERIG V TGLSE++I   +K        +  +    + 
Sbjct: 594 DRHREMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQH------KHTSSSPSSV 653

Query: 437 SMNEETNSCTICLDVIDDGTKIGILDCKHYYHADCLKQWLLIKNVCPVCKSEALK 492
            +++    C IC +   +G  +G L C H +H DC+KQW++IKN+CP+CK+EALK
Sbjct: 654 ELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTEALK 702

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5QLR54.4e-2546.67Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q7XTV72.2e-2449.57Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9ZQF91.4e-2344.35E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
O495007.7e-2243.86E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q9FMM41.1e-2042.98Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Match NameE-valueIdentityDescription
A0A0A0L2385.0e-29099.80RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G628910 P... [more]
A0A5D3DWX71.0e-26692.07Putative E3 ubiquitin-protein ligase HIP1 OS=Cucumis melo var. makuwa OX=1194695... [more]
E5GBF91.0e-26692.07Zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3CKD01.0e-26692.07uncharacterized protein LOC103501794 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3CLG51.0e-26692.07uncharacterized protein LOC103501794 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
XP_004146039.11.0e-28999.80probable E3 ubiquitin-protein ligase ZFP1 isoform X3 [Cucumis sativus] >XP_03174... [more]
KAE8649831.11.0e-28999.80hypothetical protein Csa_012901 [Cucumis sativus][more]
XP_031740109.11.0e-28999.80probable E3 ubiquitin-protein ligase ZFP1 isoform X1 [Cucumis sativus][more]
XP_011654055.11.0e-28999.80probable E3 ubiquitin-protein ligase ZFP1 isoform X2 [Cucumis sativus][more]
XP_008463711.12.1e-26692.07PREDICTED: uncharacterized protein LOC103501794 isoform X2 [Cucumis melo] >ADN33... [more]
Match NameE-valueIdentityDescription
AT1G53190.13.8e-4032.69RING/U-box superfamily protein [more]
AT1G53190.23.8e-4032.69RING/U-box superfamily protein [more]
AT3G15070.15.4e-3931.52RING/U-box superfamily protein [more]
AT3G15070.25.4e-3931.52RING/U-box superfamily protein [more]
AT2G15530.11.0e-2444.35RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 445..485
e-value: 3.7E-6
score: 36.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 443..486
e-value: 1.1E-11
score: 44.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 445..486
score: 11.899158
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 426..490
e-value: 3.7E-17
score: 63.3
NoneNo IPR availablePANTHERPTHR22937:SF65E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 9..490
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 9..490
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 444..489

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI04G23120CSPI04G23120gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G23120.1.utr3p1CSPI04G23120.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G23120.1.cds6CSPI04G23120.1.cds6CDS
CSPI04G23120.1.cds5CSPI04G23120.1.cds5CDS
CSPI04G23120.1.cds4CSPI04G23120.1.cds4CDS
CSPI04G23120.1.cds3CSPI04G23120.1.cds3CDS
CSPI04G23120.1.cds2CSPI04G23120.1.cds2CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G23120.1.utr5p2CSPI04G23120.1.utr5p2five_prime_UTR
CSPI04G23120.1.utr5p1CSPI04G23120.1.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI04G23120.1CSPI04G23120.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding