CSPI04G17360.2 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G17360.2
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	CAP-Gly domain-containing protein
Location	Chr4: 14882291 .. 14887084 (-)
Sequence length	1249
RNA-Seq Expression	CSPI04G17360.2
Synteny	CSPI04G17360.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTGCTTTAAGATTGCCCTTCTTTTTCTGTCTGTATCAGCAGTAAGGATTGTGCTTAGAGTTATCAAACGACTCTCAAATGAGTGTCTTAAGTTAAAAGAGTTAAAAGACGCTGCAAAAGAACACAACTATGGCGTCTCGATTGCAGCAGATCGAAAAGGATGAATCTGTGCTCTTGCGTGTCACCCATGCCAATCTCAAGTCCTTCACATCTGATGTTCGTTTTTCCCTTCAGGTTTGTATCATTTCTAAAGACCAGGCATCTCTTTTTAGCCTTTCCCGATTGCCCATTATGGGTTTTTATCTAAATTTGATGCTGGGGTTCTTCCATTGTAGATGAGCGTGGAGTCTGTCAAGGAAAAATTGTGGAGAAAATGTGGTACTTCTGTGAACTCTATGTGCCTCGAACTCTACGATGATTCCGGTTCTAAAATTTCCGATTTGACGGATAATTGCATTCCCTTGGGCTTCTATTCTCCCTTAGATGGGTACTTGTTCTTCTTGTTCTTCCATTTTCTTTCAATCATTTCTTTACTTGGTTTCGTTTTCCACTCTGTTAACTGCAATTCATATTGTTTCATTGATTAGAAACTCTGCTAATTTAATGGTTTTTATAAGCAGAATATGCTTTTTATGAGAATATGTGCTTTCTCTTGGAAAGGTACCGGCTGCATATTATTGATCTTGACCCCTCATCAGTTACTTCTGGTGGTTGGCTTGAAGATACCTCACTAGTGGAGAAGTTCCAGATCTCTGAAGAAGCCTATGATAAACGTGATGGTTAGTTTCTTGCCAAGCTTCCTGCATCATTAATGAAGTTTGCATGTAATTCTCTCAATTGCTTATCAAATAGGCTACGCTGAAGGCCTCCCCTCACACCCAATGACAATTGTTGGTCAGCCACAAGAATCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAGAAAGAAAGAAAAGAAGAAAAGGCTTAGGCACTGGGACCTAGGGATCTGCAGTAGACAACTACCTTCCAACTGTCTTGAATTTGCATTTCGTGGTTCTATTTAAAAAGCTTTTGGGATAGTAAAGCTGTGCTTACATATTTAAATAATGGAAGGAAAAAAGTGTAGACGATTGATCACTATATGATGCAATTGTTTTTTCTTATTGCCTTAGATTCAATCACTTGAATGCTTCATTAACTTTCCCTTCATTAGCTCTCCCTTCCTCTATAAGAACCAATTGGATACATATATTCTGTATCTCTTGTGGTTAGCAGCCTGTTGTGTGTTTCATTATTTAAATGAAATAATCCTCAAGGCATTGTGCATGGTTTACCTTCTTCTTATGGTTTCTTTTTTCAGACACATTTAGAAAATTTAAAGAAAAATTGGCATCCCAGAATCCTTCAGCATTTGAGAGTAAAGTAAGCCTCATGTTTTCTACTTTTGCTAACAATATGAATGTCCAGAGAAATTTTATTCATTTGCGCCCTGCCAGAACTATAAATTTTGATGTAGACACTACCTATTACTTTATTTAGTTCATGGCTTTTAAAGTAGAGATCTGTGCCGGGCATCTCAGTTTTTTTTTTAATTCTTTTTTATTTTATTACTTTGGTGTGTGTGTGTTCTGAATGATGATGCTGAGGCTGCATGGTATTTCTGCTCATGGTAGAATTGGAAACGTGTATCCTTCTTCTTGTCTTAGTATCCTTGAGCTCTGGTTTTTGTTCTGCGTCTCTAAGAAATTTAGGGACATGTCCATCTCTATTCTAGTCATGCTTGGTCTATTCTTACTGAGTAGCAAAATATTTGCAGATATCTGATAACTACATGGAAGAGCTCTGTGCAAATATTAAGGTTAGTTACCTTCCTTGCTTTAGAAGTGTATAATGGATGTATTCAACTATAAACTTCTTATATTAAACTTTAAAGAGTTGAATTGTCAACTGTCATGATGAAAGAAATGAATTATGTGGATCAAAATTATGTGAACGAGATTGAAAGTTAATTGACTTCCATTTTCCCCAGAGGGGTTTAGAAAATTTTTAGAATGGTGCAGTATTTGTTACTTGGTCAGGTGATTCCTAAGCGTAATTTTCCAGTCATATATTTATGTTGTCTTTTTTCTCTTTTGGAATGAGAACAACTTCTCATTAATATATGAAAAAAGGAAGAATGATCTTCAAGGATACAAGCTCCAATGGGAACAAAAATGTACAATACAAACAGAAAGGATCCTAAAAATACATGAGGATAAAACAATGAATAACACCTAGCGAAGCAATAAAAATCACAGCCAATAATCTGCATAATAGAGCACCCTCTTCAGCTTTTATGTCAGAAACTTTGAAGGAGGACAGTATAACCAAGGCACAATTGCAGCTTTTGACCCAAAATAATGTAGCCAAGAAACAATTTCTTCTGAAACACTTCTTGCATTTGATAGAGGTAGTGCCAACAGACCAAGATCTTTGGGACTGGAAAATAAAATTTCCTCTAAAACATTCTGAGTCAGCTTAAGAAGAAACCCGATATCAAAATTCATTCTAAGGAGAACACTACCATATTCGTTTGTTAAGTACCAAATTGCTTATTCTGTAAAGCAAATTGTATCAAAGTACCTAGAGTTGATTGCTTTGAAGTTTCCCAAGATCTCTAATATGCTGTGAATAATATTCGTTGAGGGAATAGTAAAAGTATTAATTAACCTTTTATCTGATGTGAATGAGGTTGAATTTCAAGCATCTGAAAACTGAAAATCTCAATAAGAGATGAAATTTTGTTAGGATTTGAGGGAGTGGACTCTTTAAGTTCATTGACTGCTGAGAGATCTCTTTCATTATTTAAAGCCAAACCACTTCATGTAAAGCTGATTCAATAACACTGGGAGAAATTACACCTTTGAAGGTGGCTACTGATCATGTCCATTTCAAATATGTTATCATTCTTTGATTAACTTTAACCTGCAAATTCCTCTCCAAGTTTCATGAAGATGAACAACTTTTGCTTCTAAGACTAGGGCAGCTGACTGAACTGCGGGAATCACAAGTCCCAGGCAATCTTCTTAGATAGTATGTGTACTAGCCTCCGAGACTTCAGCAATGTTTTTGAGAAGACACAAAGTAGACATGCTAATAAGTAATAAATGCTCCCTTTTCTTATTACCTCGTAAATCACACAGTGTAGTCAGTGTTGACTTCTGAGTTCTGATATGTAGCATTCTTTTGAGTCTTATGTCCGGAAACTATATATTATAGGTAATGTATTTACACTCTGCTCCTTGAAATCCTGTTGTCCGGTTGTACTAAACTTGACTTTTACATGATGTAGGTTGGTGATAGATGTCAAGTTGAACCCGGGGAGAAACGGGGTGTTGTGAAATTTGTTGGCCGGGCTGAATCGCTTGCTCCTGGTTTTTGGGTTGGAGTTCAATATGATGAACCATTGGGAAAAAATGATGGCACGTAAGATGACCCTTGTGATTAATTTGTTTTTCATTGTCTCTGAAGGGACGATTGACTTGTTTTTCCCAGTTCCTACTATCTGCTTCTATTCTTCCTTATAAGAAACTTTAGTTCTTGAATGTTCACTAAAATTTTTTACCCTTCCTTTTAAAGGGAGAAAAAACATTACTACGAAATATGAACATTGGAGCACATAACCTATCAATGCCCCCCTAATTTGGAAAAATTGTTTTTATTTTAGATATAGCTGTTCTGCCAGAACCGTTTTTACTCTGATCATTCTCAAATTCTTTAGGATTTTGTGCATTATATATATGGATACTTTGTATGCTACTTCTCGTGTTATAATTTGATAAAATTATCATCATTCATTTGTGTTGATTAAAAAGTTCTTATTGGTCTTAGAATCCACCTTTTACGCAATAAAGTAATCTTCCTGGTTTCAAGTTTTTCCTCCCCACTTTAGAGATTTTGATCACATATTCTGTGGGCTAAATGTGTAAAACTCCGCAGGGTAAAAGGGATCCACTATTTTGACTGCTCTCCTTTTCATGGTGCGATGGTTAGGCCTGACAAAGTGAAGGTTCGATTTTCTGCTCCATTAATCTGTTTTCCAACTTTAAAGCTTCAAAGTATGTTACATGAAATAGTTTTTTTTCCTGAACTTGGTATAATATCACATGCTTTAAATTCTTAAAAAGTAATTTATTGCTTTAGGAAAGTCAGCTTGTGGATTTGTAAATATACTACAGATGTATCTTATATTATTGGATTTCCTCAACATATTACAATATAGATGTTGATGATATATAATTAAATTTGCCTTCAACTACAGGCTTAAGCTTTTGGGTGAATTGGTGGTTTAATATGGTATCAGAGCAGGTGGTCCAAGGAGGTCCTAGTGTTCAACCCCTTGCATTGTCGTTTCCTCCCCAAGTAAAATCGATTTCCACTTGTTGGCCTTTCAAATATTTCAAGCCCACAAGTGAGGGGGAATGTTGGTGATATATAAAGAAATTTGTCTTCCAACACAAGCTTAAGCTTTTGGGTGAATTGGTGGTTTAATAATAAAAATACAATTTCTCACAAAATTCTTGATCACATATGATCAATCAAACTTATAGCAACAGAAAACACAAACAACTCTAAACAGAAATGCAAGAAAGAAAAAATCACAAAGAACTAAGATCCAACATTAGGGACCTGGATCCTTCTTCCTCTCTTGGTGCGCAAGGAGGAATGCAGCCTCTGCTTCTCCAGAAAATATCTTCAATGCTCTCCCCCTTCTCATTCCTTTTCTTTTATTCTAA

mRNA sequence

CTTTTGCTTTAAGATTGCCCTTCTTTTTCTGTCTGTATCAGCAGTAAGGATTGTGCTTAGAGTTATCAAACGACTCTCAAATGAGTGTCTTAAGTTAAAAGAGTTAAAAGACGCTGCAAAAGAACACAACTATGGCGTCTCGATTGCAGCAGATCGAAAAGGATGAATCTGTGCTCTTGCGTGTCACCCATGCCAATCTCAAGTCCTTCACATCTGATGTTCGTTTTTCCCTTCAGATGAGCGTGGAGTCTGTCAAGGAAAAATTGTGGAGAAAATGTGGTACTTCTGTGAACTCTATGTGCCTCGAACTCTACGATGATTCCGGTTCTAAAATTTCCGATTTGACGGATAATTGCATTCCCTTGGGCTTCTATTCTCCCTTAGATGGGTACTTGTTCTTCTTGTTCTTCCATTTTCTTTCAATCATTTCTTTACTTGGTTTCGTTTTCCACTCTGTTAACTGCAATTCATATTGTTTCATTGATTAGAAACTCTGCTAATTTAATGGTTTTTATAAGCAGAATATGCTTTTTATGAGAATATGTGCTTTCTCTTGGAAAGGTACCGGCTGCATATTATTGATCTTGACCCCTCATCAGTTACTTCTGGTGGTTGGCTTGAAGATACCTCACTAGTGGAGAAGTTCCAGATCTCTGAAGAAGCCTATGATAAACGTGATGACACATTTAGAAAATTTAAAGAAAAATTGGCATCCCAGAATCCTTCAGCATTTGAGAGTAAAATATCTGATAACTACATGGAAGAGCTCTGTGCAAATATTAAGGTTGGTGATAGATGTCAAGTTGAACCCGGGGAGAAACGGGGTGTTGTGAAATTTGTTGGCCGGGCTGAATCGCTTGCTCCTGGTTTTTGGGTTGGAGTTCAATATGATGAACCATTGGGAAAAAATGATGGCACGGTAAAAGGGATCCACTATTTTGACTGCTCTCCTTTTCATGGTGCGATGGTTAGGCCTGACAAAGTGAAGGTTCGATTTTCTGCTCCATTAATCTGTTTTCCAACTTTAAAGCTTCAAAGCTTAAGCTTTTGGGTGAATTGGTGGTTTAATATGGTATCAGAGCAGGTGGTCCAAGGAGGTCCTAGTGTTCAACCCCTTGCATTGTCGTTTCCTCCCCAAGGACCTGGATCCTTCTTCCTCTCTTGGTGCGCAAGGAGGAATGCAGCCTCTGCTTCTCCAGAAAATATCTTCAATGCTCTCCCCCTTCTCATTCCTTTTCTTTTATTCTAA

Coding sequence (CDS)

ATGTGCTTTCTCTTGGAAAGGTACCGGCTGCATATTATTGATCTTGACCCCTCATCAGTTACTTCTGGTGGTTGGCTTGAAGATACCTCACTAGTGGAGAAGTTCCAGATCTCTGAAGAAGCCTATGATAAACGTGATGACACATTTAGAAAATTTAAAGAAAAATTGGCATCCCAGAATCCTTCAGCATTTGAGAGTAAAATATCTGATAACTACATGGAAGAGCTCTGTGCAAATATTAAGGTTGGTGATAGATGTCAAGTTGAACCCGGGGAGAAACGGGGTGTTGTGAAATTTGTTGGCCGGGCTGAATCGCTTGCTCCTGGTTTTTGGGTTGGAGTTCAATATGATGAACCATTGGGAAAAAATGATGGCACGGTAAAAGGGATCCACTATTTTGACTGCTCTCCTTTTCATGGTGCGATGGTTAGGCCTGACAAAGTGAAGGTTCGATTTTCTGCTCCATTAATCTGTTTTCCAACTTTAAAGCTTCAAAGCTTAAGCTTTTGGGTGAATTGGTGGTTTAATATGGTATCAGAGCAGGTGGTCCAAGGAGGTCCTAGTGTTCAACCCCTTGCATTGTCGTTTCCTCCCCAAGGACCTGGATCCTTCTTCCTCTCTTGGTGCGCAAGGAGGAATGCAGCCTCTGCTTCTCCAGAAAATATCTTCAATGCTCTCCCCCTTCTCATTCCTTTTCTTTTATTCTAA

Protein sequence

MCFLLERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKNDGTVKGIHYFDCSPFHGAMVRPDKVKVRFSAPLICFPTLKLQSLSFWVNWWFNMVSEQVVQGGPSVQPLALSFPPQGPGSFFLSWCARRNAASASPENIFNALPLLIPFLLF*

Homology

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BLAST of CSPI04G17360.2 vs. ExPASy Swiss-Prot
Match: Q67Z52 (Tubulin-folding cofactor B OS=Arabidopsis thaliana OX=3702 GN=TFCB PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 8.1e-62
Identity = 110/143 (76.92%), Postives = 124/143 (86.71%), Query Frame = 0


Query: 8   YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAFESK 67
           +RLHIIDLDPSSVT+GGWLEDTSLVEK+ ISEE Y KR D+FRKFKEK  SQNP A E+K
Sbjct: 87  FRLHIIDLDPSSVTTGGWLEDTSLVEKYNISEEDYAKRTDSFRKFKEKRVSQNPVAAEAK 146

Query: 68  ISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKNDGTV 127
             +NYME+LCANIKVGDRCQVEPGEKRG+VK+VGRAESL PG+WVG+QYDEPLGK+DG V
Sbjct: 147 TKENYMEDLCANIKVGDRCQVEPGEKRGMVKYVGRAESLGPGYWVGIQYDEPLGKHDGMV 206

Query: 128 KGIHYFDCSPFHGAMVRPDKVKV 151
           KG  +F+C    G MVRPDKVKV
Sbjct: 207 KGTRFFECPRLQGGMVRPDKVKV 229

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BLAST of CSPI04G17360.2 vs. ExPASy Swiss-Prot
Match: Q20728 (Tubulin-specific chaperone B OS=Caenorhabditis elegans OX=6239 GN=tbcb-1 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.9e-27
Identity = 68/148 (45.95%), Postives = 91/148 (61.49%), Query Frame = 0


Query: 8   YRLHIIDLDPSSVTSGGW-LEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAFE- 67
           YR+H +D     VT G    +D S+VEK+++S++ Y KR D+ R +K+K+  +  SA   
Sbjct: 77  YRIHAVD-----VTGGNEDFKDESMVEKYEMSDDTYGKRTDSVRAWKKKMQEEQGSAAPM 136

Query: 68  SKISDNYMEELCANIKVGDRCQVEPG---EKRGVVKFVGRAESLAPGFWVGVQYDEPLGK 127
              SD   EE   NI VG+RC+V  G    +RG V +VG A     G WVGV+YDEP+GK
Sbjct: 137 ENESDKLNEEAAKNIMVGNRCEVTVGAQMARRGEVAYVG-ATKFKEGVWVGVKYDEPVGK 196

Query: 128 NDGTVKGIHYFDCSPFHGAMVRPDKVKV 151
           NDG+V G+ YFDC P +G  VRP  VKV
Sbjct: 197 NDGSVAGVRYFDCDPKYGGFVRPVDVKV 218

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy Swiss-Prot
Match: Q5E951 (Tubulin-folding cofactor B OS=Bos taurus OX=9913 GN=TBCB PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 6.7e-24
Identity = 63/152 (41.45%), Postives = 91/152 (59.87%), Query Frame = 0


Query: 9   RLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKF--KEKLASQNPSAFES 68
           R+H+ID   + +   G  ED S VEK++IS+EAY++R D+ R F  + KL   N      
Sbjct: 84  RIHVIDHSGARL---GEYEDISKVEKYEISQEAYEQRQDSIRSFLKRNKLGRFNEEERAQ 143

Query: 69  KISDNYM-----EELCANIKVGDRCQVE-PGE--KRGVVKFVGRAESLAPGFWVGVQYDE 128
           + ++N       E   + I VG RC+V  PG+  +RG V +VG  +   PG+W+G++YDE
Sbjct: 144 QEAENSQRLIEEEAQASTIPVGSRCEVRTPGQPPRRGTVMYVGLTD-FKPGYWIGIRYDE 203

Query: 129 PLGKNDGTVKGIHYFDCSPFHGAMVRPDKVKV 151
           PLGKNDG+V G  YF+C   +GA V+P  V V
Sbjct: 204 PLGKNDGSVNGKRYFECQAKYGAFVKPSVVTV 231

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy Swiss-Prot
Match: Q99426 (Tubulin-folding cofactor B OS=Homo sapiens OX=9606 GN=TBCB PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 65/152 (42.76%), Postives = 90/152 (59.21%), Query Frame = 0


Query: 9   RLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKF--KEKLASQNP---SA 68
           R+H+ID   + +   G  ED S VEK+ IS+EAYD+R DT R F  + KL   N    + 
Sbjct: 84  RIHVIDHSGARL---GEYEDVSRVEKYTISQEAYDQRQDTVRSFLKRSKLGRYNEEERAQ 143

Query: 69  FESKISDNYMEE--LCANIKVGDRCQVE---PGEKRGVVKFVGRAESLAPGFWVGVQYDE 128
            E++ +    EE    ++I VG RC+V       +RG V +VG  +   PG+W+GV+YDE
Sbjct: 144 QEAEAAQRLAEEKAQASSIPVGSRCEVRAAGQSPRRGTVMYVGLTD-FKPGYWIGVRYDE 203

Query: 129 PLGKNDGTVKGIHYFDCSPFHGAMVRPDKVKV 151
           PLGKNDG+V G  YF+C   +GA V+P  V V
Sbjct: 204 PLGKNDGSVNGKRYFECQAKYGAFVKPAVVTV 231

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy Swiss-Prot
Match: Q9D1E6 (Tubulin-folding cofactor B OS=Mus musculus OX=10090 GN=Tbcb PE=1 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 2.0e-23
Identity = 64/154 (41.56%), Postives = 91/154 (59.09%), Query Frame = 0


Query: 9   RLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKF---------KEKLASQ 68
           R+H+I  D S V  G + ED S VEK++IS EAY++R +T R F          E+L +Q
Sbjct: 84  RIHVI--DHSGVRLGEY-EDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQ 143

Query: 69  NPSAFESKISDNYMEELCANIKVGDRCQVEPGE---KRGVVKFVGRAESLAPGFWVGVQY 128
             +    ++S+   +   + I VG RC+V   +   +RG V +VG  +   PG+WVGV+Y
Sbjct: 144 QEAEAAQRLSEEKAQ--ASAISVGSRCEVRAPDHSLRRGTVMYVGLTD-FKPGYWVGVRY 203

Query: 129 DEPLGKNDGTVKGIHYFDCSPFHGAMVRPDKVKV 151
           DEPLGKNDG+V G  YF+C   +GA V+P  V V
Sbjct: 204 DEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTV 231

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy TrEMBL
Match: A0A0A0L346 (CAP-Gly domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G354120 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 2.1e-89
Identity = 162/174 (93.10%), Postives = 168/174 (96.55%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKVRFSAPLICFPTLKLQSLSFWVNWWFNMV 179
           GTVKGIHYFDCSPFHGAMVRPDKVKVRFSAPLICFPTLKLQS+   + ++ N+V
Sbjct: 204 GTVKGIHYFDCSPFHGAMVRPDKVKVRFSAPLICFPTLKLQSMLHEIVFFLNLV 257

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy TrEMBL
Match: A0A1S3C118 (tubulin-folding cofactor B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495804 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 9.9e-87
Identity = 157/162 (96.91%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKVRFSAPLICFPTLKLQS 167
           GTVKGIHYFDCSP HGAMVRPDKVKV+FSAPLICFPTLKL+S
Sbjct: 204 GTVKGIHYFDCSPLHGAMVRPDKVKVQFSAPLICFPTLKLRS 245

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy TrEMBL
Match: A0A1S3C1L1 (tubulin-folding cofactor B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495804 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 6.4e-78
Identity = 143/146 (97.95%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKV 151
           GTVKGIHYFDCSP HGAMVRPDKVKV
Sbjct: 204 GTVKGIHYFDCSPLHGAMVRPDKVKV 229

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy TrEMBL
Match: A0A6J1DKD2 (tubulin-folding cofactor B OS=Momordica charantia OX=3673 GN=LOC111021286 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.5e-74
Identity = 137/146 (93.84%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEK+QISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISD YMEELCANIKVGDRC+VEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDTYMEELCANIKVGDRCEVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKV 151
           G VKGI YFDC P HGAMVRPDKVKV
Sbjct: 204 GMVKGIRYFDCPPLHGAMVRPDKVKV 229

[Close]

BLAST of CSPI04G17360.2 vs. ExPASy TrEMBL
Match: A0A6J1HTU0 (tubulin-folding cofactor B OS=Cucurbita maxima OX=3661 GN=LOC111466096 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.5e-71
Identity = 133/146 (91.10%), Postives = 138/146 (94.52%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEK+QISEEAY+KRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEAYEKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEELCANIKVGDRC+VEPG+ RG VKFVG AESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEELCANIKVGDRCEVEPGKIRGSVKFVGWAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKV 151
           G VKGI YFDC P HGAMVRPDKVKV
Sbjct: 204 GMVKGIRYFDCPPLHGAMVRPDKVKV 229

[Close]

BLAST of CSPI04G17360.2 vs. NCBI nr
Match: XP_008455690.1 (PREDICTED: tubulin-folding cofactor B isoform X1 [Cucumis melo])

HSP 1 Score: 329.7 bits (844), Expect = 2.0e-86
Identity = 157/162 (96.91%), Postives = 160/162 (98.77%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKVRFSAPLICFPTLKLQS 167
           GTVKGIHYFDCSP HGAMVRPDKVKV+FSAPLICFPTLKL+S
Sbjct: 204 GTVKGIHYFDCSPLHGAMVRPDKVKVQFSAPLICFPTLKLRS 245

[Close]

BLAST of CSPI04G17360.2 vs. NCBI nr
Match: XP_004142648.1 (tubulin-folding cofactor B [Cucumis sativus] >KAE8649611.1 hypothetical protein Csa_012598 [Cucumis sativus])

HSP 1 Score: 302.8 bits (774), Expect = 2.7e-78
Identity = 144/146 (98.63%), Postives = 145/146 (99.32%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKV 151
           GTVKGIHYFDCSPFHGAMVRPDKVKV
Sbjct: 204 GTVKGIHYFDCSPFHGAMVRPDKVKV 229

[Close]

BLAST of CSPI04G17360.2 vs. NCBI nr
Match: XP_008455691.1 (PREDICTED: tubulin-folding cofactor B isoform X2 [Cucumis melo])

HSP 1 Score: 300.4 bits (768), Expect = 1.3e-77
Identity = 143/146 (97.95%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKV 151
           GTVKGIHYFDCSP HGAMVRPDKVKV
Sbjct: 204 GTVKGIHYFDCSPLHGAMVRPDKVKV 229

[Close]

BLAST of CSPI04G17360.2 vs. NCBI nr
Match: XP_022153874.1 (tubulin-folding cofactor B [Momordica charantia])

HSP 1 Score: 288.5 bits (737), Expect = 5.2e-74
Identity = 137/146 (93.84%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEK+QISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISD YMEELCANIKVGDRC+VEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDTYMEELCANIKVGDRCEVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKV 151
           G VKGI YFDC P HGAMVRPDKVKV
Sbjct: 204 GMVKGIRYFDCPPLHGAMVRPDKVKV 229

[Close]

BLAST of CSPI04G17360.2 vs. NCBI nr
Match: XP_038889058.1 (tubulin-folding cofactor B [Benincasa hispida])

HSP 1 Score: 285.8 bits (730), Expect = 3.4e-73
Identity = 135/146 (92.47%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 5   LERYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 64
           L+ YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF
Sbjct: 84  LDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAF 143

Query: 65  ESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 124
           ESKISDNYMEE+CAN+KVGDRC+VEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND
Sbjct: 144 ESKISDNYMEEICANMKVGDRCEVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKND 203

Query: 125 GTVKGIHYFDCSPFHGAMVRPDKVKV 151
           G VKGI YFDC   HGAM+RPDKVKV
Sbjct: 204 GMVKGIRYFDCPLLHGAMIRPDKVKV 229

[Close]

BLAST of CSPI04G17360.2 vs. TAIR 10
Match: AT3G10220.1 (tubulin folding cofactor B )

HSP 1 Score: 238.4 bits (607), Expect = 5.8e-63
Identity = 110/143 (76.92%), Postives = 124/143 (86.71%), Query Frame = 0


Query: 8   YRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEAYDKRDDTFRKFKEKLASQNPSAFESK 67
           +RLHIIDLDPSSVT+GGWLEDTSLVEK+ ISEE Y KR D+FRKFKEK  SQNP A E+K
Sbjct: 87  FRLHIIDLDPSSVTTGGWLEDTSLVEKYNISEEDYAKRTDSFRKFKEKRVSQNPVAAEAK 146

Query: 68  ISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKNDGTV 127
             +NYME+LCANIKVGDRCQVEPGEKRG+VK+VGRAESL PG+WVG+QYDEPLGK+DG V
Sbjct: 147 TKENYMEDLCANIKVGDRCQVEPGEKRGMVKYVGRAESLGPGYWVGIQYDEPLGKHDGMV 206

Query: 128 KGIHYFDCSPFHGAMVRPDKVKV 151
           KG  +F+C    G MVRPDKVKV
Sbjct: 207 KGTRFFECPRLQGGMVRPDKVKV 229

The following BLAST results are available for this feature:

BLAST of CSPI04G17360.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67Z52	8.1e-62	76.92	Tubulin-folding cofactor B OS=Arabidopsis thaliana OX=3702 GN=TFCB PE=1 SV=1	[more]
Q20728	2.9e-27	45.95	Tubulin-specific chaperone B OS=Caenorhabditis elegans OX=6239 GN=tbcb-1 PE=1 SV...	[more]
Q5E951	6.7e-24	41.45	Tubulin-folding cofactor B OS=Bos taurus OX=9913 GN=TBCB PE=2 SV=1	[more]
Q99426	1.1e-23	42.76	Tubulin-folding cofactor B OS=Homo sapiens OX=9606 GN=TBCB PE=1 SV=2	[more]
Q9D1E6	2.0e-23	41.56	Tubulin-folding cofactor B OS=Mus musculus OX=10090 GN=Tbcb PE=1 SV=2	[more]

BLAST of CSPI04G17360.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L346	2.1e-89	93.10	CAP-Gly domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G354120 PE=...	[more]
A0A1S3C118	9.9e-87	96.91	tubulin-folding cofactor B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495804 PE...	[more]
A0A1S3C1L1	6.4e-78	97.95	tubulin-folding cofactor B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495804 PE...	[more]
A0A6J1DKD2	2.5e-74	93.84	tubulin-folding cofactor B OS=Momordica charantia OX=3673 GN=LOC111021286 PE=4 S...	[more]
A0A6J1HTU0	1.5e-71	91.10	tubulin-folding cofactor B OS=Cucurbita maxima OX=3661 GN=LOC111466096 PE=4 SV=1	[more]

BLAST of CSPI04G17360.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455690.1	2.0e-86	96.91	PREDICTED: tubulin-folding cofactor B isoform X1 [Cucumis melo]	[more]
XP_004142648.1	2.7e-78	98.63	tubulin-folding cofactor B [Cucumis sativus] >KAE8649611.1 hypothetical protein ...	[more]
XP_008455691.1	1.3e-77	97.95	PREDICTED: tubulin-folding cofactor B isoform X2 [Cucumis melo]	[more]
XP_022153874.1	5.2e-74	93.84	tubulin-folding cofactor B [Momordica charantia]	[more]
XP_038889058.1	3.4e-73	92.47	tubulin-folding cofactor B [Benincasa hispida]	[more]

BLAST of CSPI04G17360.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10220.1	5.8e-63	76.92	tubulin folding cofactor B	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000938	CAP Gly-rich domain	SMART	SM01052	CAP_GLY_2	coord: 82..149 e-value: 2.0E-29 score: 113.7
IPR000938	CAP Gly-rich domain	PFAM	PF01302	CAP_GLY	coord: 82..148 e-value: 1.1E-22 score: 79.7
IPR000938	CAP Gly-rich domain	PROSITE	PS00845	CAP_GLY_1	coord: 101..133
IPR000938	CAP Gly-rich domain	PROSITE	PS50245	CAP_GLY_2	coord: 102..144 score: 17.04879
IPR036859	CAP Gly-rich domain superfamily	GENE3D	2.30.30.190		coord: 76..155 e-value: 7.4E-28 score: 98.1
IPR036859	CAP Gly-rich domain superfamily	SUPERFAMILY	74924	Cap-Gly domain	coord: 24..150
None	No IPR available	PANTHER	PTHR18916	DYNACTIN 1-RELATED MICROTUBULE-BINDING	coord: 8..150
None	No IPR available	PANTHER	PTHR18916:SF76	TUBULIN FOLDING COFACTOR B	coord: 8..150

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G17360	CSPI04G17360	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G17360.2.cds8	CSPI04G17360.2.cds8	CDS
CSPI04G17360.2.cds7	CSPI04G17360.2.cds7	CDS
CSPI04G17360.2.cds6	CSPI04G17360.2.cds6	CDS
CSPI04G17360.2.cds5	CSPI04G17360.2.cds5	CDS
CSPI04G17360.2.cds4	CSPI04G17360.2.cds4	CDS
CSPI04G17360.2.cds3	CSPI04G17360.2.cds3	CDS
CSPI04G17360.2.cds2	CSPI04G17360.2.cds2	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G17360.2.utr5p2	CSPI04G17360.2.utr5p2	five_prime_UTR
CSPI04G17360.2.utr5p1	CSPI04G17360.2.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G17360.2	CSPI04G17360.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0009793	embryo development ending in seed dormancy
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005515	protein binding