CSPI03G39940.4 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI03G39940.4
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCarbonic anhydrase
LocationChr3: 34226346 .. 34228882 (-)
Sequence length1657
RNA-Seq ExpressionCSPI03G39940.4
SyntenyCSPI03G39940.4
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGCTTTTTGCTCCACATACCAAAAATATGTTTTCTTCTAGTCAATATCATTATATATGAATATGTATATGTATATGTATGTGTATATATATACTTCTTGTTTGTTGCTAAGTTTTTTATTGGGGAAATTGAATCTTGTAGACAGAAGATATGGCGAATGGTTCATATGAGGAAGCCATTGAAGCTTTGGAGAAGCTCCTCAGGTACCTTGAATTCAATATATTTTTTTTATTTAAGTACTTTTAATTATACCTTATTTATTTTATTTTGTGTATTTATTGGTGGGTGTAGAGAAAAGGGAGATTTGAAGGCAACAGCAACATCAAAAGTGGCACAAATAACAGCAGAACTAAAAACTGCAGATGGAGAAAAGCCAGCCTTTGATCCTGTTGAGAGAATCAAAACTGGTTTCATTCACTTCAAGAAGGAGAAATATGAGTAAATCCTTCAATTTCCCTTTTAGTAGACATGTTTATTTAATTACTTACTTCTATATATGTGTTCTCCCAAACAAATCGATTTTTACTCGTCATTTGACTGTAGTTTTCAAAACCGAATCATACTTCGGTCTCATTTGGTGACTTCTCTCTTTGTTTTCCAGCAAGAACCCAGAGTTGTATGGTGAACTTGCAAAAGGCCAAAGCCCCAAGGTATTTATCACTCTTTTTTTTTATTGGGACATTTTTCATTTGGTATGGTATGAGTTAATTGAAGAAGGGTGATCATTTAGAACTGAATTTAATTTTATAATGTCATGGAAAATGCAGTTTATGGTGTTTGCTTGCTCTGATTCAAGAGTGTGCCCATCCCATGTTCTTGATTTCCAACCTGGGGAGGCTTTTGTGGTCAGAAATGTTGCCAATTTGGTTCCTCCTTATGATCAGGTTTTGTTTTAGTTATACTTTAAATTAATTAATAATTTTTTCTTCCATGTTGTATGTGTTTAAATATGAATATTGGGCTGTTTCTTTTCGTTTAAAACAGGACAAATATTCAGGAACTGGATCTGCCATTGAATATGCTGTTCTTCATCTCAAGGTATTATTATAAACAACTTCAAATATGCTATTTTTTAATTCATAATATAGATTTAGTGATGAATGATTTGGTTTTGGTGTTGGTGTTGTAGGTGCAATACATTGTTGTGATTGGTCACAGTGCCTGTGGTGGTATTAAGGGGCTTATGACTTTCCCATATGATGGAAAGTACTCAACGTAAGCCTTCTTCTTCTCCATATTCTCAAAACCTATAATTTAGGATGAATAGTGACTTTATTCACAAAACAGTGCTATATTTGAAAATATACTGAACAAGAACAACCAAAAGAAACTTTATAAAACATAAAAGTAGCTTATCCAAACATAACGTTGCTCTAAAATTGGTAATAAACTGATTAGAACTCACATCTGTGATTGAAAACAGTGATTTCATTGAAGAATGGGTGAAAGTTGGTTTGCCTGCTAAGGCCAAGGTCAATTCAGCCCATGGTGGTGCAGACCTTGGGGAACTTTGTACACACTGTGAAAAGGTAAATAAAATAAAAGCAATTTTGGCACTTCATATTCAAATAGAGAATAAGAAAGTGAACAAATGGGATTTCTGTTATTAACATTTGTTTCTTTCATTTATTGGAAAATGCAGGAAGCAGTTGCTGTATCACTTGGGAATCTGCTAACCTATCCATTTGTTAGGGATGGTTTGGTGAATGGAACTCTTGGCCTCAAGGGTGGTCACTACGACTTCATTAACGGAACTTTCGAGCTCTGGGGACTCGAATTCAACGTTACTAAGCCTTTGTCTGTATGAACTCCATCTATTACCGGCGGATCGATCTGGTTTTTCTCACTGATTTTCTTCATGTGATGTACTTTCTTTGTGTAATCGCCTTTTCTAGTATATAAATTGACAATAAATATGAGATGGAGTTCATTAGAAACTCCAGTTCTAAAAGGTTACCTTTTCTTCTCCCTTTTCTGTCATTTCTTTCAATGAGAATGATGCAGAATTTTAAAACTTCTTTTTCTTTTTCTTTCTTTTGCTTTTTACTTTTCACCTTTTTACTCTCTACCAAAAATGCTACTCTTTCTTTGTTAGGTAAAGGATGTGGCCACCATTCTGCACTGGAAGCTCTAGGGACATGTTAAGAAGTCAATCCTGTAAAGATGAGACTTCTTTTTAATAACACACTCTTTGGTTATGTTGAAATAAGGCTGTTTCTTTTCTTCCTGAGGATTGAATCTACCCTTCAATTTGATTGAAACCCGTTTCAGATTCACCCGTATTCTAGGAGTTCCATTTCCTCTCTTTCCTTTCTATTGCTTCGAATTGCATTCAAAATGTTAGATTTCGTTTAAGAGCATATAAGAGTAATACTTGGGTGTAGCCCCTGCTGCACCCTTCTTCACTTCATGCATCTCCTTCAAGGATAAAGTAAATATATAATCTATATTTAAAGAAAACTTTGTTCTTAGCTTGTGATTGAGCATTTGGGTGAGCTGCACAAATACAAGTGCAGCTCCTGATGCAACCAGAGC

mRNA sequence

TTTTGCTTTTTGCTCCACATACCAAAAATATGTTTTCTTCTAGTCAATATCATTATATATGAATATGTATATGTATATGTATGTGTATATATATACTTCTTGTTTGTTGCTAAGTTTTTTATTGGGGAAATTGAATCTTGTAGACAGAAGATATGGCGAATGGTTCATATGAGGAAGCCATTGAAGCTTTGGAGAAGCTCCTCAGAGAAAAGGGAGATTTGAAGGCAACAGCAACATCAAAAGTGGCACAAATAACAGCAGAACTAAAAACTGCAGATGGAGAAAAGCCAGCCTTTGATCCTGTTGAGAGAATCAAAACTGGTTTCATTCACTTCAAGAAGGAGAAATATGACAAGAACCCAGAGTTGTATGGTGAACTTGCAAAAGGCCAAAGCCCCAAGTTTATGGTGTTTGCTTGCTCTGATTCAAGAGTGTGCCCATCCCATGTTCTTGATTTCCAACCTGGGGAGGCTTTTGTGGTCAGAAATGTTGCCAATTTGGTTCCTCCTTATGATCAGGACAAATATTCAGGAACTGGATCTGCCATTGAATATGCTGTTCTTCATCTCAAGGTGCAATACATTGTTGTGATTGGTCACAGTGCCTGTGGTGGTATTAAGGGGCTTATGACTTTCCCATATGATGGAAAGTACTCAACTGATTTCATTGAAGAATGGGTGAAAGTTGGTTTGCCTGCTAAGGCCAAGGTCAATTCAGCCCATGGTGGTGCAGACCTTGGGGAACTTTGTACACACTGTGAAAAGGAAGCAGTTGCTGTATCACTTGGGAATCTGCTAACCTATCCATTTGTTAGGGATGGTTTGGTGAATGGAACTCTTGGCCTCAAGGGTGGTCACTACGACTTCATTAACGGAACTTTCGAGCTCTGGGGACTCGAATTCAACGTTACTAAGCCTTTGTCTGTATGAACTCCATCTATTACCGGCGGATCGATCTGGTTTTTCTCACTGATTTTCTTCATGTGATGTACTTTCTTTGTGTAATCGCCTTTTCTAGTATATAAATTGACAATAAATATGAGATGGAGTTCATTAGAAACTCCAGTTCTAAAAGGTTACCTTTTCTTCTCCCTTTTCTGTCATTTCTTTCAATGAGAATGATGCAGAATTTTAAAACTTCTTTTTCTTTTTCTTTCTTTTGCTTTTTACTTTTCACCTTTTTACTCTCTACCAAAAATGCTACTCTTTCTTTGTTAGGTAAAGGATGTGGCCACCATTCTGCACTGGAAGCTCTAGGGACATGTTAAGAAGTCAATCCTGTAAAGATGAGACTTCTTTTTAATAACACACTCTTTGGTTATGTTGAAATAAGGCTGTTTCTTTTCTTCCTGAGGATTGAATCTACCCTTCAATTTGATTGAAACCCGTTTCAGATTCACCCGTATTCTAGGAGTTCCATTTCCTCTCTTTCCTTTCTATTGCTTCGAATTGCATTCAAAATGTTAGATTTCGTTTAAGAGCATATAAGAGTAATACTTGGGTGTAGCCCCTGCTGCACCCTTCTTCACTTCATGCATCTCCTTCAAGGATAAAGTAAATATATAATCTATATTTAAAGAAAACTTTGTTCTTAGCTTGTGATTGAGCATTTGGGTGAGCTGCACAAATACAAGTGCAGCTCCTGATGCAACCAGAGC

Coding sequence (CDS)

ATGGCGAATGGTTCATATGAGGAAGCCATTGAAGCTTTGGAGAAGCTCCTCAGAGAAAAGGGAGATTTGAAGGCAACAGCAACATCAAAAGTGGCACAAATAACAGCAGAACTAAAAACTGCAGATGGAGAAAAGCCAGCCTTTGATCCTGTTGAGAGAATCAAAACTGGTTTCATTCACTTCAAGAAGGAGAAATATGACAAGAACCCAGAGTTGTATGGTGAACTTGCAAAAGGCCAAAGCCCCAAGTTTATGGTGTTTGCTTGCTCTGATTCAAGAGTGTGCCCATCCCATGTTCTTGATTTCCAACCTGGGGAGGCTTTTGTGGTCAGAAATGTTGCCAATTTGGTTCCTCCTTATGATCAGGACAAATATTCAGGAACTGGATCTGCCATTGAATATGCTGTTCTTCATCTCAAGGTGCAATACATTGTTGTGATTGGTCACAGTGCCTGTGGTGGTATTAAGGGGCTTATGACTTTCCCATATGATGGAAAGTACTCAACTGATTTCATTGAAGAATGGGTGAAAGTTGGTTTGCCTGCTAAGGCCAAGGTCAATTCAGCCCATGGTGGTGCAGACCTTGGGGAACTTTGTACACACTGTGAAAAGGAAGCAGTTGCTGTATCACTTGGGAATCTGCTAACCTATCCATTTGTTAGGGATGGTTTGGTGAATGGAACTCTTGGCCTCAAGGGTGGTCACTACGACTTCATTAACGGAACTTTCGAGCTCTGGGGACTCGAATTCAACGTTACTAAGCCTTTGTCTGTATGA

Protein sequence

MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIHFKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGLPAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFINGTFELWGLEFNVTKPLSV*
Homology
BLAST of CSPI03G39940.4 vs. ExPASy Swiss-Prot
Match: P17067 (Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 3.4e-114
Identity = 198/255 (77.65%), Postives = 217/255 (85.10%), Query Frame = 0

Query: 6   YEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEK--PAFDPVERIKTGFIHFKK 65
           Y+EAIE L+KLLREK +LKATA  KV QITA+L T       P  +  ERIKTGF+HFKK
Sbjct: 74  YDEAIEELQKLLREKTELKATAAEKVEQITAQLGTTSSSDGIPKSEASERIKTGFLHFKK 133

Query: 66  EKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQD 125
           EKYDKNP LYGELAKGQSP FMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQ 
Sbjct: 134 EKYDKNPALYGELAKGQSPPFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQA 193

Query: 126 KYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGLPAK 185
           KY+GTG+AIEYAVLHLKV  IVVIGHSACGGIKGL++FP+DG YSTDFIEEWVK+GLPAK
Sbjct: 194 KYAGTGAAIEYAVLHLKVSNIVVIGHSACGGIKGLLSFPFDGTYSTDFIEEWVKIGLPAK 253

Query: 186 AKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFINGTF 245
           AKV + HG A   ELCTHCEKEAV  SLGNLLTYPFVR+GLVN TL LKGG+YDF+ G+F
Sbjct: 254 AKVKAQHGDAPFAELCTHCEKEAVNASLGNLLTYPFVREGLVNKTLALKGGYYDFVKGSF 313

Query: 246 ELWGLEFNVTKPLSV 259
           ELWGLEF ++   SV
Sbjct: 314 ELWGLEFGLSSTFSV 328

BLAST of CSPI03G39940.4 vs. ExPASy Swiss-Prot
Match: P27141 (Carbonic anhydrase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.7e-108
Identity = 190/258 (73.64%), Postives = 215/258 (83.33%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           MA  SYE+AI ALEKLL EKG+L   A ++V QITAEL+++DG KP FDPVE +K GFIH
Sbjct: 65  MAKESYEQAIAALEKLLSEKGELGPIAAARVDQITAELQSSDGSKP-FDPVEHMKAGFIH 124

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FK EKY+KNP LYGEL+KGQSPKFMVFACSDSRVCPSHVL+FQPGEAFVVRN+AN+VP Y
Sbjct: 125 FKTEKYEKNPALYGELSKGQSPKFMVFACSDSRVCPSHVLNFQPGEAFVVRNIANMVPAY 184

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           D+ +YSG G+AIEYAVLHLKV+ IVVIGHSACGGIKGLM+ P DG  ST FIE+WVK+GL
Sbjct: 185 DKTRYSGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSLPADGSESTAFIEDWVKIGL 244

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKV   H      + CT CEKEAV VSLGNLLTYPFVR+GLV  TL LKGGHYDF+N
Sbjct: 245 PAKAKVQGEHVDKCFADQCTACEKEAVNVSLGNLLTYPFVREGLVKKTLALKGGHYDFVN 304

Query: 241 GTFELWGLEFNVTKPLSV 259
           G FELWGLEF ++  LSV
Sbjct: 305 GGFELWGLEFGLSPSLSV 321

BLAST of CSPI03G39940.4 vs. ExPASy Swiss-Prot
Match: P46512 (Carbonic anhydrase 1 OS=Flaveria linearis OX=4225 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 3.5e-106
Identity = 184/256 (71.88%), Postives = 212/256 (82.81%), Query Frame = 0

Query: 3   NGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIHFK 62
           N SYEEAI+AL+K+L EKG+L+  A +++ QITA+    D + P FDPVERIK+GF+ FK
Sbjct: 76  NESYEEAIDALKKMLIEKGELEPVAAARIDQITAQAAAPDTKAP-FDPVERIKSGFVKFK 135

Query: 63  KEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQ 122
            EK+  NP LY ELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVAN+VPP+D+
Sbjct: 136 TEKFVTNPALYDELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANMVPPFDK 195

Query: 123 DKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGLPA 182
            KYSG G+A+EYAVLHLKVQ I VIGHS CGGIKGLMTFP +G +STDFIE+WVKV LPA
Sbjct: 196 TKYSGVGAAVEYAVLHLKVQEIFVIGHSRCGGIKGLMTFPDEGPHSTDFIEDWVKVCLPA 255

Query: 183 KAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFINGT 242
           K+KV + H G  L + C  CEKEAV VSLGNLLTYPFVRDGL N TL LKGGHYDF+NGT
Sbjct: 256 KSKVVAEHNGTHLDDQCVQCEKEAVNVSLGNLLTYPFVRDGLRNNTLALKGGHYDFVNGT 315

Query: 243 FELWGLEFNVTKPLSV 259
           FELW L+F ++ P SV
Sbjct: 316 FELWALDFGLSSPTSV 330

BLAST of CSPI03G39940.4 vs. ExPASy Swiss-Prot
Match: P46511 (Carbonic anhydrase OS=Flaveria brownii OX=33111 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 7.7e-106
Identity = 184/256 (71.88%), Postives = 212/256 (82.81%), Query Frame = 0

Query: 3   NGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIHFK 62
           N SYEEAI+AL+K+L EKG+L+  A +++ QITA+    D + P FDPVERIK+GF+ FK
Sbjct: 76  NESYEEAIDALKKMLIEKGELEPVAAARIDQITAQAAAPDTKAP-FDPVERIKSGFVKFK 135

Query: 63  KEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQ 122
            EK+  NP LY ELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVAN+VPP+D+
Sbjct: 136 TEKFVTNPALYDELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANMVPPFDK 195

Query: 123 DKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGLPA 182
            KYSG G+A+EYAVLHLKVQ I VIGHS CGGIKGLMTFP +G +STDFIE+WVKV LPA
Sbjct: 196 TKYSGVGAAVEYAVLHLKVQEIFVIGHSRCGGIKGLMTFPDEGPHSTDFIEDWVKVCLPA 255

Query: 183 KAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFINGT 242
           K+KV + H G  L + C  CEKEAV VSLGNLLTYPFVRDGL N TL LKGGHYDF+NGT
Sbjct: 256 KSKVVAEHNGTHLDDQCVLCEKEAVNVSLGNLLTYPFVRDGLRNNTLALKGGHYDFVNGT 315

Query: 243 FELWGLEFNVTKPLSV 259
           FELW L+F ++ P SV
Sbjct: 316 FELWALDFGLSSPTSV 330

BLAST of CSPI03G39940.4 vs. ExPASy Swiss-Prot
Match: P46281 (Carbonic anhydrase OS=Flaveria pringlei OX=4226 PE=2 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.0e-105
Identity = 184/256 (71.88%), Postives = 212/256 (82.81%), Query Frame = 0

Query: 3   NGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIHFK 62
           N SYEEAI+AL+K+L EKG+L+  A +++ QITA+    D + P FDPVERIK+GF+ FK
Sbjct: 75  NESYEEAIDALKKMLIEKGELEPVAAARIDQITAQAAAPDTKAP-FDPVERIKSGFVKFK 134

Query: 63  KEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQ 122
            EK+  NP LY ELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVAN+VPP+D+
Sbjct: 135 TEKFVTNPVLYDELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANMVPPFDK 194

Query: 123 DKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGLPA 182
            KYSG G+A+EYAVLHLKVQ I VIGHS CGGIKGLMTFP +G +STDFIE+WVKV LPA
Sbjct: 195 TKYSGVGAAVEYAVLHLKVQEIFVIGHSRCGGIKGLMTFPDEGPHSTDFIEDWVKVCLPA 254

Query: 183 KAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFINGT 242
           K+KV + H G  L + C  CEKEAV VSLGNLLTYPFVRDGL N TL LKGGHYDF+NGT
Sbjct: 255 KSKVVAEHNGTHLDDQCVLCEKEAVNVSLGNLLTYPFVRDGLRNNTLALKGGHYDFVNGT 314

Query: 243 FELWGLEFNVTKPLSV 259
           FELW L+F ++ P SV
Sbjct: 315 FELWALDFGLSSPTSV 329

BLAST of CSPI03G39940.4 vs. ExPASy TrEMBL
Match: A0A0A0LD02 (Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G836520 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.2e-148
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH
Sbjct: 73  MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 132

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 133 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 192

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 193 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 252

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 253 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 312

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 313 GTFELWGLEFNVTKPLSV 330

BLAST of CSPI03G39940.4 vs. ExPASy TrEMBL
Match: A0A1S3B881 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103487089 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 7.1e-147
Identity = 255/258 (98.84%), Postives = 256/258 (99.22%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           M NGSYEEAIEALEKLLREKGDLKATATSKV QITAELKTADGEKPAFDPVERIKTGFIH
Sbjct: 75  MGNGSYEEAIEALEKLLREKGDLKATATSKVEQITAELKTADGEKPAFDPVERIKTGFIH 134

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 135 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 194

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 195 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 254

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGA+LGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 255 PAKAKVNSAHGGAELGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 314

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 315 GTFELWGLEFNVTKPLSV 332

BLAST of CSPI03G39940.4 vs. ExPASy TrEMBL
Match: A0A1S3B7R9 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103487089 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 7.1e-147
Identity = 255/258 (98.84%), Postives = 256/258 (99.22%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           M NGSYEEAIEALEKLLREKGDLKATATSKV QITAELKTADGEKPAFDPVERIKTGFIH
Sbjct: 75  MGNGSYEEAIEALEKLLREKGDLKATATSKVEQITAELKTADGEKPAFDPVERIKTGFIH 134

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 135 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 194

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 195 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 254

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGA+LGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 255 PAKAKVNSAHGGAELGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 314

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 315 GTFELWGLEFNVTKPLSV 332

BLAST of CSPI03G39940.4 vs. ExPASy TrEMBL
Match: A0A5A7UJK4 (Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00430 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.1e-146
Identity = 254/258 (98.45%), Postives = 255/258 (98.84%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           M NGSYEEAIEALEKLLREKGDLKATATSKV QITAELKT DGEKPAFDPVERIKTGFIH
Sbjct: 1   MGNGSYEEAIEALEKLLREKGDLKATATSKVEQITAELKTVDGEKPAFDPVERIKTGFIH 60

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGA+LGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 181 PAKAKVNSAHGGAELGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 241 GTFELWGLEFNVTKPLSV 258

BLAST of CSPI03G39940.4 vs. ExPASy TrEMBL
Match: A0A6J1F8Y2 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111441908 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.3e-136
Identity = 235/258 (91.09%), Postives = 244/258 (94.57%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           M NGSYEEAI+ALEKLLREKGDLKATATSKV QITAELKT DG KP FDPVERIK+GFIH
Sbjct: 68  MGNGSYEEAIQALEKLLREKGDLKATATSKVEQITAELKTPDGGKPPFDPVERIKSGFIH 127

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 128 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 187

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQ K+SGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFP+DG YSTDFIEEWVK+GL
Sbjct: 188 DQSKFSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPFDGNYSTDFIEEWVKIGL 247

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKV S HGGADLGE+C HCEKEAV VSLGNLLTYPFVRDGLVNGTLGLKGGHYDF+N
Sbjct: 248 PAKAKVKSQHGGADLGEMCGHCEKEAVGVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFVN 307

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGL+F+ T  LSV
Sbjct: 308 GTFELWGLDFSFTPSLSV 325

BLAST of CSPI03G39940.4 vs. NCBI nr
Match: XP_004141091.2 (carbonic anhydrase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 534.6 bits (1376), Expect = 4.6e-148
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH
Sbjct: 73  MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 132

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 133 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 192

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 193 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 252

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 253 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 312

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 313 GTFELWGLEFNVTKPLSV 330

BLAST of CSPI03G39940.4 vs. NCBI nr
Match: XP_011652279.1 (carbonic anhydrase 2 isoform X1 [Cucumis sativus] >KAE8651253.1 hypothetical protein Csa_001060 [Cucumis sativus])

HSP 1 Score: 534.6 bits (1376), Expect = 4.6e-148
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH
Sbjct: 73  MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 132

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 133 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 192

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 193 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 252

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 253 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 312

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 313 GTFELWGLEFNVTKPLSV 330

BLAST of CSPI03G39940.4 vs. NCBI nr
Match: XP_008443520.1 (PREDICTED: carbonic anhydrase 2-like isoform X2 [Cucumis melo])

HSP 1 Score: 529.6 bits (1363), Expect = 1.5e-146
Identity = 255/258 (98.84%), Postives = 256/258 (99.22%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           M NGSYEEAIEALEKLLREKGDLKATATSKV QITAELKTADGEKPAFDPVERIKTGFIH
Sbjct: 75  MGNGSYEEAIEALEKLLREKGDLKATATSKVEQITAELKTADGEKPAFDPVERIKTGFIH 134

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 135 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 194

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 195 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 254

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGA+LGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 255 PAKAKVNSAHGGAELGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 314

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 315 GTFELWGLEFNVTKPLSV 332

BLAST of CSPI03G39940.4 vs. NCBI nr
Match: XP_008443519.1 (PREDICTED: carbonic anhydrase 2-like isoform X1 [Cucumis melo])

HSP 1 Score: 529.6 bits (1363), Expect = 1.5e-146
Identity = 255/258 (98.84%), Postives = 256/258 (99.22%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           M NGSYEEAIEALEKLLREKGDLKATATSKV QITAELKTADGEKPAFDPVERIKTGFIH
Sbjct: 75  MGNGSYEEAIEALEKLLREKGDLKATATSKVEQITAELKTADGEKPAFDPVERIKTGFIH 134

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 135 FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 194

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 195 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 254

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGA+LGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 255 PAKAKVNSAHGGAELGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 314

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 315 GTFELWGLEFNVTKPLSV 332

BLAST of CSPI03G39940.4 vs. NCBI nr
Match: KAA0053671.1 (carbonic anhydrase 2-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 528.1 bits (1359), Expect = 4.3e-146
Identity = 254/258 (98.45%), Postives = 255/258 (98.84%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTADGEKPAFDPVERIKTGFIH 60
           M NGSYEEAIEALEKLLREKGDLKATATSKV QITAELKT DGEKPAFDPVERIKTGFIH
Sbjct: 1   MGNGSYEEAIEALEKLLREKGDLKATATSKVEQITAELKTVDGEKPAFDPVERIKTGFIH 60

Query: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120
           FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY
Sbjct: 61  FKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPY 120

Query: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180
           DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL
Sbjct: 121 DQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVGL 180

Query: 181 PAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240
           PAKAKVNSAHGGA+LGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN
Sbjct: 181 PAKAKVNSAHGGAELGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFIN 240

Query: 241 GTFELWGLEFNVTKPLSV 259
           GTFELWGLEFNVTKPLSV
Sbjct: 241 GTFELWGLEFNVTKPLSV 258

BLAST of CSPI03G39940.4 vs. TAIR 10
Match: AT3G01500.2 (carbonic anhydrase 1 )

HSP 1 Score: 377.9 bits (969), Expect = 6.7e-105
Identity = 183/259 (70.66%), Postives = 212/259 (81.85%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKT-ADGEKPAFDPVERIKTGFI 60
           M   +Y+EAIEAL+KLL EK +LK  A +KV QITA L+T    +K AFDPVE IK GFI
Sbjct: 78  MGTEAYDEAIEALKKLLIEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFI 137

Query: 61  HFKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPP 120
            FKKEKY+ NP LYGELAKGQSPK+MVFACSDSRVCPSHVLDFQPG+AFVVRN+AN+VPP
Sbjct: 138 KFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPP 197

Query: 121 YDQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVG 180
           +D+ KY G G+AIEYAVLHLKV+ IVVIGHSACGGIKGLM+FP DG  STDFIE+WVK+ 
Sbjct: 198 FDKVKYGGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKIC 257

Query: 181 LPAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFI 240
           LPAK+KV S  G +   + C  CE+EAV VSL NLLTYPFVR+GLV GTL LKGG+YDF+
Sbjct: 258 LPAKSKVISELGDSAFEDQCGRCEREAVNVSLANLLTYPFVREGLVKGTLALKGGYYDFV 317

Query: 241 NGTFELWGLEFNVTKPLSV 259
            G FELWGLEF +++  SV
Sbjct: 318 KGAFELWGLEFGLSETSSV 336

BLAST of CSPI03G39940.4 vs. TAIR 10
Match: AT3G01500.1 (carbonic anhydrase 1 )

HSP 1 Score: 377.9 bits (969), Expect = 6.7e-105
Identity = 183/259 (70.66%), Postives = 212/259 (81.85%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKT-ADGEKPAFDPVERIKTGFI 60
           M   +Y+EAIEAL+KLL EK +LK  A +KV QITA L+T    +K AFDPVE IK GFI
Sbjct: 1   MGTEAYDEAIEALKKLLIEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFI 60

Query: 61  HFKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPP 120
            FKKEKY+ NP LYGELAKGQSPK+MVFACSDSRVCPSHVLDFQPG+AFVVRN+AN+VPP
Sbjct: 61  KFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPP 120

Query: 121 YDQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVG 180
           +D+ KY G G+AIEYAVLHLKV+ IVVIGHSACGGIKGLM+FP DG  STDFIE+WVK+ 
Sbjct: 121 FDKVKYGGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKIC 180

Query: 181 LPAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFI 240
           LPAK+KV S  G +   + C  CE+EAV VSL NLLTYPFVR+GLV GTL LKGG+YDF+
Sbjct: 181 LPAKSKVISELGDSAFEDQCGRCEREAVNVSLANLLTYPFVREGLVKGTLALKGGYYDFV 240

Query: 241 NGTFELWGLEFNVTKPLSV 259
            G FELWGLEF +++  SV
Sbjct: 241 KGAFELWGLEFGLSETSSV 259

BLAST of CSPI03G39940.4 vs. TAIR 10
Match: AT3G01500.3 (carbonic anhydrase 1 )

HSP 1 Score: 377.9 bits (969), Expect = 6.7e-105
Identity = 183/259 (70.66%), Postives = 212/259 (81.85%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKT-ADGEKPAFDPVERIKTGFI 60
           M   +Y+EAIEAL+KLL EK +LK  A +KV QITA L+T    +K AFDPVE IK GFI
Sbjct: 78  MGTEAYDEAIEALKKLLIEKEELKTVAAAKVEQITAALQTGTSSDKKAFDPVETIKQGFI 137

Query: 61  HFKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPP 120
            FKKEKY+ NP LYGELAKGQSPK+MVFACSDSRVCPSHVLDFQPG+AFVVRN+AN+VPP
Sbjct: 138 KFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFQPGDAFVVRNIANMVPP 197

Query: 121 YDQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVG 180
           +D+ KY G G+AIEYAVLHLKV+ IVVIGHSACGGIKGLM+FP DG  STDFIE+WVK+ 
Sbjct: 198 FDKVKYGGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKIC 257

Query: 181 LPAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFI 240
           LPAK+KV S  G +   + C  CE+EAV VSL NLLTYPFVR+GLV GTL LKGG+YDF+
Sbjct: 258 LPAKSKVISELGDSAFEDQCGRCEREAVNVSLANLLTYPFVREGLVKGTLALKGGYYDFV 317

Query: 241 NGTFELWGLEFNVTKPLSV 259
            G FELWGLEF +++  SV
Sbjct: 318 KGAFELWGLEFGLSETSSV 336

BLAST of CSPI03G39940.4 vs. TAIR 10
Match: AT5G14740.1 (carbonic anhydrase 2 )

HSP 1 Score: 372.9 bits (956), Expect = 2.1e-103
Identity = 178/259 (68.73%), Postives = 213/259 (82.24%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTA-DGEKPAFDPVERIKTGFI 60
           M N SYE+AIEAL+KLL EK DLK  A +KV +ITAEL+ A   +  +FDPVERIK GF+
Sbjct: 73  MGNESYEDAIEALKKLLIEKDDLKDVAAAKVKKITAELQAASSSDSKSFDPVERIKEGFV 132

Query: 61  HFKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPP 120
            FKKEKY+ NP LYGELAKGQSPK+MVFACSDSRVCPSHVLDF PG+AFVVRN+AN+VPP
Sbjct: 133 TFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPP 192

Query: 121 YDQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVG 180
           +D+ KY+G G+AIEYAVLHLKV+ IVVIGHSACGGIKGLM+FP DG  STDFIE+WVK+ 
Sbjct: 193 FDKVKYAGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKIC 252

Query: 181 LPAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFI 240
           LPAK+KV +    +   + C  CE+EAV VSL NLLTYPFVR+G+V GTL LKGG+YDF+
Sbjct: 253 LPAKSKVLAESESSAFEDQCGRCEREAVNVSLANLLTYPFVREGVVKGTLALKGGYYDFV 312

Query: 241 NGTFELWGLEFNVTKPLSV 259
           NG+FELW L+F ++   S+
Sbjct: 313 NGSFELWELQFGISPVHSI 331

BLAST of CSPI03G39940.4 vs. TAIR 10
Match: AT5G14740.2 (carbonic anhydrase 2 )

HSP 1 Score: 372.9 bits (956), Expect = 2.1e-103
Identity = 178/259 (68.73%), Postives = 213/259 (82.24%), Query Frame = 0

Query: 1   MANGSYEEAIEALEKLLREKGDLKATATSKVAQITAELKTA-DGEKPAFDPVERIKTGFI 60
           M N SYE+AIEAL+KLL EK DLK  A +KV +ITAEL+ A   +  +FDPVERIK GF+
Sbjct: 1   MGNESYEDAIEALKKLLIEKDDLKDVAAAKVKKITAELQAASSSDSKSFDPVERIKEGFV 60

Query: 61  HFKKEKYDKNPELYGELAKGQSPKFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPP 120
            FKKEKY+ NP LYGELAKGQSPK+MVFACSDSRVCPSHVLDF PG+AFVVRN+AN+VPP
Sbjct: 61  TFKKEKYETNPALYGELAKGQSPKYMVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPP 120

Query: 121 YDQDKYSGTGSAIEYAVLHLKVQYIVVIGHSACGGIKGLMTFPYDGKYSTDFIEEWVKVG 180
           +D+ KY+G G+AIEYAVLHLKV+ IVVIGHSACGGIKGLM+FP DG  STDFIE+WVK+ 
Sbjct: 121 FDKVKYAGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSFPLDGNNSTDFIEDWVKIC 180

Query: 181 LPAKAKVNSAHGGADLGELCTHCEKEAVAVSLGNLLTYPFVRDGLVNGTLGLKGGHYDFI 240
           LPAK+KV +    +   + C  CE+EAV VSL NLLTYPFVR+G+V GTL LKGG+YDF+
Sbjct: 181 LPAKSKVLAESESSAFEDQCGRCEREAVNVSLANLLTYPFVREGVVKGTLALKGGYYDFV 240

Query: 241 NGTFELWGLEFNVTKPLSV 259
           NG+FELW L+F ++   S+
Sbjct: 241 NGSFELWELQFGISPVHSI 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P170673.4e-11477.65Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1[more]
P271411.7e-10873.64Carbonic anhydrase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P465123.5e-10671.88Carbonic anhydrase 1 OS=Flaveria linearis OX=4225 PE=2 SV=1[more]
P465117.7e-10671.88Carbonic anhydrase OS=Flaveria brownii OX=33111 PE=2 SV=1[more]
P462811.0e-10571.88Carbonic anhydrase OS=Flaveria pringlei OX=4226 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LD022.2e-148100.00Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_3G836520 PE=3 SV=1[more]
A0A1S3B8817.1e-14798.84Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103487089 PE=3 SV=1[more]
A0A1S3B7R97.1e-14798.84Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103487089 PE=3 SV=1[more]
A0A5A7UJK42.1e-14698.45Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G0... [more]
A0A6J1F8Y23.3e-13691.09Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111441908 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141091.24.6e-148100.00carbonic anhydrase 2 isoform X2 [Cucumis sativus][more]
XP_011652279.14.6e-148100.00carbonic anhydrase 2 isoform X1 [Cucumis sativus] >KAE8651253.1 hypothetical pro... [more]
XP_008443520.11.5e-14698.84PREDICTED: carbonic anhydrase 2-like isoform X2 [Cucumis melo][more]
XP_008443519.11.5e-14698.84PREDICTED: carbonic anhydrase 2-like isoform X1 [Cucumis melo][more]
KAA0053671.14.3e-14698.45carbonic anhydrase 2-like isoform X2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G01500.26.7e-10570.66carbonic anhydrase 1 [more]
AT3G01500.16.7e-10570.66carbonic anhydrase 1 [more]
AT3G01500.36.7e-10570.66carbonic anhydrase 1 [more]
AT5G14740.12.1e-10368.73carbonic anhydrase 2 [more]
AT5G14740.22.1e-10368.73carbonic anhydrase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 6..26
NoneNo IPR availablePANTHERPTHR11002:SF56BETA CARBONIC ANHYDRASE 2, CHLOROPLASTICcoord: 1..258
NoneNo IPR availablePANTHERPTHR11002CARBONIC ANHYDRASEcoord: 1..258
IPR001765Carbonic anhydraseSMARTSM00947Pro_CA_2coord: 77..247
e-value: 9.3E-52
score: 187.9
IPR001765Carbonic anhydrasePFAMPF00484Pro_CAcoord: 85..242
e-value: 3.2E-40
score: 138.0
IPR036874Carbonic anhydrase superfamilyGENE3D3.40.1050.10Carbonic anhydrasecoord: 39..258
e-value: 5.5E-73
score: 246.9
IPR036874Carbonic anhydrase superfamilySUPERFAMILY53056beta-carbonic anhydrase, cabcoord: 50..256
IPR015892Carbonic anhydrase, prokaryotic-like, conserved sitePROSITEPS00705PROK_CO2_ANHYDRASE_2coord: 133..153
IPR015892Carbonic anhydrase, prokaryotic-like, conserved sitePROSITEPS00704PROK_CO2_ANHYDRASE_1coord: 89..96
IPR045066Beta carbonic anhydrases, cladeBCDDcd00884beta_CA_cladeBcoord: 57..246
e-value: 3.71392E-100
score: 288.286

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI03G39940CSPI03G39940gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G39940.4.utr3p1CSPI03G39940.4.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G39940.4.cds8CSPI03G39940.4.cds8CDS
CSPI03G39940.4.cds7CSPI03G39940.4.cds7CDS
CSPI03G39940.4.cds6CSPI03G39940.4.cds6CDS
CSPI03G39940.4.cds5CSPI03G39940.4.cds5CDS
CSPI03G39940.4.cds4CSPI03G39940.4.cds4CDS
CSPI03G39940.4.cds3CSPI03G39940.4.cds3CDS
CSPI03G39940.4.cds2CSPI03G39940.4.cds2CDS
CSPI03G39940.4.cds1CSPI03G39940.4.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G39940.4.utr5p1CSPI03G39940.4.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI03G39940.4CSPI03G39940.4-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015976 carbon utilization
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding