CSPI03G28780.1 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI03G28780.1
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionVacuolar cation/proton exchanger
LocationChr3: 26384200 .. 26389657 (-)
Sequence length2081
RNA-Seq ExpressionCSPI03G28780.1
SyntenyCSPI03G28780.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTGTCTGAACTTGAAGAAGTTTACGTCTAATTATTCTGTTTTTATTATTACTTTTTAAAAATACATACTTTATGTAATGTAATGCAATAAATAAACAAGTCAAAATTAAATGGGGAAGACGCAAGAGGACGATATTTCGCAGAAGCTTCCTAGCTGCTGTTGAGTTTCGCTATATTGCCTTCCCCTTCCCATCAGCCATCCAACTCGACTTCTTCTTCGCTTGATCTATAATTTTGGTGAAGTCGAAAATCTTATTTCGAAGAACTTTCCTTCTGGCTGGCCTTCGCCGGCTGTGTTGGCCAGAGATTTTATTTTTAAGCAACTGAAATTACCTATACGATCCAAATTAGTGGGACCCGATTGCTTCTTGTCTTATTTTCTCCCCTTGCGTTTGAGGGTTGGAATCTCAGCTCGATTTGGGGAGTTCTTGATCATTCTCCAGCTGGGATTCGACCTGGATATCCTCGTTGCGTCAGTTCAAGTTCATCTCCAATCTGGTAAATATCCTCTTGCTCTTTTTCTTGTTTGGGTAACGAAATCTTGTTTTAATCTCGACTCGATCTGATTTCTTCTTTCTGTTGAGTTAATATTGCTTTGGATTTGACTTGTGGAAGATGAAGTAGCTGATGAGTTGTATATGTAAATCAGCCCAGATTTGTATTTTTAGTGTAGTGGAAAGGTATAATTGATCGGGATAGCTGTGGTTGTAGATGGGATCAACAGAGGAGAAAACAAATTACTTATCAGATGAAGAAATTCCATTGAAGTCATCGCCAGTTGCGAAGAACGTATTGCATAGTTTTGATTGTGAGACGGCTGCTGTGCGTTCTTCCCCAGATACAAGATGGTGGAAGACTAGCAACATTCGTATTAGACCTCTTAGAAGTGCTTATATAGTGTTGGTTAGAGCAAAGATCAACGTGTTGCTACCTTTTGGTCCTTTGGCAATTCTGCTACATTACTTAACGGGAAAGCATGTAAGAACTACTCCTCTTCATAATTTTACTGGTTGATTTTCTGCAATAGTAAATGACATGGTTGGATTTGAGAAGAAAGAAATGGTGAAAGACTTGTATTGGGTAACTTTTTCATGGAACAGAAGCTCCTAGACAATCAAAGACAGTTTCTCATTATTTGATTTAATGAAGATATGGTCTATGACCATCTTCTATTGACCTGTTTATATTATTTTGGATATTAAAAAATTCAGCTTTTAAGATCGTTTTGCTGGTTAGTACTTTGGCTTGTATGATTTGTCTCATGGTTCATAATTCCTTGATAGTCAGTTTTATATGTCAGGGATGGGTCTTCTTTTTCACTTTACTTGGAATTACACCCTTGGCAGAACGTCTTGGTTATGCAACTGAGTAAGATTTGGTTTCACATATTCTATGTTTTAGATTTCATATTATTCCATTTGTGCTACACTTATTTTTCAAAAATATTTGTGACAGGCAGCTTGCTTTCTACACTGGACCAACAGGTTTGAGTGCTACCTGATCAGGCATTGCTTGTTTCGATCATTTAATGGTGGCTTTTTGGTTAGTTTCCTTAATAACTCAAATGCCAATGGTTTTTGCAGTTGGTGGTCTTTTGAATGCAACATTTGGAAATGCAACTGAAATGATCATATCAATATATGCGTTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCATTGCTGGGGTCAATCTTGTCAAATATGCTCCTTGTGCTTGGTTGCGCTTTTTTCACTGGTGGGATTATTCATCATACAAAGGTGCAGGTTTTCAATAAGGTACAGGGTTTCGTTCTCTTGGGCATTATGCTTTACCTCTATTTTAGAGATATTTTACTTGCACCTTATTAGTTTACATTAAGATATATGACTATTTTGCAACAGGGTGCTGCTGTTGTAAATTCAGGATTGTTGTTGATGGCTGTAATGGGAACGACTTTTCCGGCCGTGCTTCATTTCACCCACACAGAACTTCATTTGGGAGAGTCAGCATTATCTCTTTCAAGATTTAGCAGTTGTATAATGTTAATTGCATATGCAAGCTACCTTTTCTTTCAACTAAAAAGTCAGCATGATCTCTATGGTCCTCTTGACGAGGTGAGTCTCTTTGGATCTATGTTTGGATCTTGCTTTTAATTATTGTCTTTAGTTAACAAGCAATTGTTCTTCACTTGGCTTCTTTCATCTGTAACATACTATAATATTTTCAACTATTAGGGCGAATTAGGAATCAGGATTTGCAACATCTATAAGAGGCGAGCTTGAAACGATGGCTAGGAAACTGTTTATTTTATTTTTTTCAAGAAGAAATAAAAAAGAGAACTAGTACCATCAATAATAAATGCTCATGTGACGGTAACATAATTATCATACATAACCTCAATCACCTAGTTGTTTTACATATGATAACTTTAACTGGACTAGTGGGGAATGCATCCTTTAGGAATGGGAACTTAACAAATGAGGCACAAATCAATTGGTTTAGCTTTTCAAGTGTTTAATTTAAAAAATAATTCATTTGGGAGGCAACCACTCAAAATGACTTTTAGAGAACTTTCAAACTTTATTTTAAACAGGTTTTTATAAAAAAAAGTTCAAATGGAAATGAGTTTTGATAAACACTTTCTTCTCTAGTCAATCCAAATATGTCTTTAGGGGCGCTTTTTATTTGTTAAGTAGTCCCCACACTTAAAAAATGCAGAATCAAAGAAATGATCAACACTTTTTTTTGTTCACTCTAGAACTCCATTTTAAGCAGATATGCATTTAATTCTAATGGTTTTAGCCTTTTCTTATATTTCTTTAGGAAGTTGATGGAGAAGTTGAGGACGATTCTGAGATTTATGCTTGGGAAGCAATTGGATGGCTTGCCATCTTGACTGTATGGGTATCTGTTTTATCTGGATACCTTGTTGATGCTATCCAGGTAAATCTTATTGTGATTGCTTTTAAATTATTAACTATAATTATTTGCATTCTATTATTGTTTGCTTTGCTTGTCCATCCATGCGCTGACTCTGATTAAGTTCTCGGTAGCTCAATGCTTGTTTTTTTAAGTGAAATTTATGAACCTTTTTCCTTTTTCTAGAAGAATAGCAGTAAAGAAGATATGGAAGAACAAAAGATGTTTTTTCAAATATCAAATATTAGTTAATTCGATCCATATAAAGCAGTTATCATAAAACATATCCAAAGATTCATGTGACAATATCTTGATGTTTGGTGCTTAATATTGGTTTCTCGTATCCAGTAAATTTAAGTAGAAGTTTGTGGATCTTTTTCTCAGGGAGCATCTGAATCATTAAATTTGCCAGTGGCATTTATTAGTGTAATTTTGTTGCCAATTGTTGGAAATGCTGCTGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTGTGAGCACTTTCTGGATTTATTAAATTTTATTATTTTCTTCCAATCATCTCTTTTGTTTGGTTTGATTGACGTAAAAGTTATTGTAGGACATCACTCTTGGAGTTGCTATTGGATCATCTACACAAATATCCATGTTTGTGGTATGTGTTGCGGATTATGTTTTAGGGTCCATTTGATTGACACTCTCCTTCTTAGTTTTTCCTTTCTTGATTTTTTTTTTTTTACTTTAAAAAAATCAGAATCGGAAATGTTTTTATAGTATTGAATTATATAACATGTATTAAAACAAATATAAATATTTTAAAAAGACTTTATGGTATTGAATATAATTTCACCAAAGCAAAATAATAATAATAATTGAAACTTTCTTCAACTAAAGTTCAATTTTGATTTGACAGGTTATCCCACACTCTTATCTTAGATAATGTTGTCTTACCTTACTTGAGTTTATTCAATTAGAATAATTTATAAATTTTATTTTTATCTCTCCTTACCAAATGTTTTTGCTTTTCATGACTCAATTGTATACACGAATAAAATCTAAAAAAGAGAAAATTTACTTTTTTTTGTTTTACTATTTTCGAACATATCAAGATGAAAACAAATAGAATAAAATTTTAGATATATATCATTGATAGAATCTAAAATTTACTACATTTTATAAATATTTTCAATAGTTTTGCATTGAGGGAAAGTAATAGAGATTTGAGAAAATAAACATAGCTGAAGGCTGTTTAATGAAGAGAGAAAAAAGAATGATAAGGCTTAGAGAGAAGAAGAAAGAAAAGGAAAAGAAAGTTTTAAGAGAAAAAATGCATTGGAAGAAAGAAGAGATAAACGTGTGGTACATGTATTAAAACAGGAGAAAGGGAAAAAAAAAAAACTTAGTGTTTGTTATTTTTTCTCAAATTTGGCCAATTTTCTTTGGAAAACGTACCTTAAACTTAAAACAAGAAACAAAAATAATTATCATATAAGTTGCTCCTTAAGAAACAAGCAATGAAAACAAGAAACAATCTTATCAAATGTGTTCTAAATTGCGGTTGATTTTAACCCTAGATCTTAAAAACTGTTGTGTTGGCTTGGAAATTGATTTTGTACTGGTAACTTGACTGTGTGTTATTGATGTGTGTAGATCCCGTTTTGCGTAGTAGTTGGATGGTGCATGGGAAAACCAATGGACTTAAACTTCAAACTCTTTGAAACAGCAACGTTGTTCATCACTGTGCTAGTAGTGGCCTTTATGTTGCAGGTGTGATATCTTCAATCTATACTCCTGTAATTAAATTCATTTGCAATTTAATAGCTATCCTTAGTACTATTACTTTGAAATTTTTGGTGCATTTCCATTAATGAGTTTTCATTTATATGCAAAGCACCATTCATAGTAGTAGAACGATTGATATCTTAGTTTTGTATAACTCGGCATTGGTCAAATTTGCAGGAGGGGACATCAAATTATTTCAAAGGTCTGATGCTTATTCTATGTTATCTCATAGTGGCAGCCAGTTTTTTTGTACACGTTGATCCTAGTAATGGTGAGTAGTTACTTCTTGAATCCACATACAAGCTGGACTCGTCCATCTCGTTTTCTAAATTTACTGTGTTTTTGGTAACATCATCTTTCTTGTTTCTCGTTTATCAAACACATGCCCTATGATTGCTTCTGGTTTATTGGTCTGCTGTACAAAGTAAACATTTGTTTGTTAATTCTGAGCTCATTATTATTTATGCATTGCTAATCAGATGATTAACACTCGGAGAACGTTTGGTCATGGAAATTTACTGCAAGCTGAACTTTTGCATTTGCATTGGTTCTGAAACCCCACATTGCTTCAGTTCGTTGGAACCTGCAAACATTAGAGGACTCACACAAATGGTTTGTCTTTAAATGGTGTTTGGTTTCATTAGTTGAACTGTACTGTAATCTTTTTGTGGCTTGACAGTTGATCTTTTCTCTTCTTTTGTCATCTTCCTCTTTAACTTATTTATCATAAACTTGAGAGCTGTAATCTATATTCATTAATTGTAAGCTCTTTCTTTTATTGTTC

mRNA sequence

GTTTTGTCTGAACTTGAAGAAGTTTACGTCTAATTATTCTGTTTTTATTATTACTTTTTAAAAATACATACTTTATGTAATGTAATGCAATAAATAAACAAGTCAAAATTAAATGGGGAAGACGCAAGAGGACGATATTTCGCAGAAGCTTCCTAGCTGCTGTTGAGTTTCGCTATATTGCCTTCCCCTTCCCATCAGCCATCCAACTCGACTTCTTCTTCGCTTGATCTATAATTTTGGTGAAGTCGAAAATCTTATTTCGAAGAACTTTCCTTCTGGCTGGCCTTCGCCGGCTGTGTTGGCCAGAGATTTTATTTTTAAGCAACTGAAATTACCTATACGATCCAAATTAGTGGGACCCGATTGCTTCTTGTCTTATTTTCTCCCCTTGCGTTTGAGGGTTGGAATCTCAGCTCGATTTGGGGAGTTCTTGATCATTCTCCAGCTGGGATTCGACCTGGATATCCTCGTTGCGTCAGTTCAAGTTCATCTCCAATCTGATGGGATCAACAGAGGAGAAAACAAATTACTTATCAGATGAAGAAATTCCATTGAAGTCATCGCCAGTTGCGAAGAACGTATTGCATAGTTTTGATTGTGAGACGGCTGCTGTGCGTTCTTCCCCAGATACAAGATGGTGGAAGACTAGCAACATTCGTATTAGACCTCTTAGAAGTGCTTATATAGTGTTGGTTAGAGCAAAGATCAACGTGTTGCTACCTTTTGGTCCTTTGGCAATTCTGCTACATTACTTAACGGGAAAGCATGGATGGGTCTTCTTTTTCACTTTACTTGGAATTACACCCTTGGCAGAACGTCTTGGTTATGCAACTGAGCAGCTTGCTTTCTACACTGGACCAACAGTTGGTGGTCTTTTGAATGCAACATTTGGAAATGCAACTGAAATGATCATATCAATATATGCGTTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCATTGCTGGGGTCAATCTTGTCAAATATGCTCCTTGTGCTTGGTTGCGCTTTTTTCACTGGTGGGATTATTCATCATACAAAGGTGCAGGTTTTCAATAAGGGTGCTGCTGTTGTAAATTCAGGATTGTTGTTGATGGCTGTAATGGGAACGACTTTTCCGGCCGTGCTTCATTTCACCCACACAGAACTTCATTTGGGAGAGTCAGCATTATCTCTTTCAAGATTTAGCAGTTGTATAATGTTAATTGCATATGCAAGCTACCTTTTCTTTCAACTAAAAAGTCAGCATGATCTCTATGGTCCTCTTGACGAGGAAGTTGATGGAGAAGTTGAGGACGATTCTGAGATTTATGCTTGGGAAGCAATTGGATGGCTTGCCATCTTGACTGTATGGGTATCTGTTTTATCTGGATACCTTGTTGATGCTATCCAGGGAGCATCTGAATCATTAAATTTGCCAGTGGCATTTATTAGTGTAATTTTGTTGCCAATTGTTGGAAATGCTGCTGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTGACATCACTCTTGGAGTTGCTATTGGATCATCTACACAAATATCCATGTTTGTGATCCCGTTTTGCGTAGTAGTTGGATGGTGCATGGGAAAACCAATGGACTTAAACTTCAAACTCTTTGAAACAGCAACGTTGTTCATCACTGTGCTAGTAGTGGCCTTTATGTTGCAGGAGGGGACATCAAATTATTTCAAAGGTCTGATGCTTATTCTATGTTATCTCATAGTGGCAGCCAGTTTTTTTGTACACGTTGATCCTAGTAATGATGATTAACACTCGGAGAACGTTTGGTCATGGAAATTTACTGCAAGCTGAACTTTTGCATTTGCATTGGTTCTGAAACCCCACATTGCTTCAGTTCGTTGGAACCTGCAAACATTAGAGGACTCACACAAATGGTTTGTCTTTAAATGGTGTTTGGTTTCATTAGTTGAACTGTACTGTAATCTTTTTGTGGCTTGACAGTTGATCTTTTCTCTTCTTTTGTCATCTTCCTCTTTAACTTATTTATCATAAACTTGAGAGCTGTAATCTATATTCATTAATTGTAAGCTCTTTCTTTTATTGTTC

Coding sequence (CDS)

ATGGGATCAACAGAGGAGAAAACAAATTACTTATCAGATGAAGAAATTCCATTGAAGTCATCGCCAGTTGCGAAGAACGTATTGCATAGTTTTGATTGTGAGACGGCTGCTGTGCGTTCTTCCCCAGATACAAGATGGTGGAAGACTAGCAACATTCGTATTAGACCTCTTAGAAGTGCTTATATAGTGTTGGTTAGAGCAAAGATCAACGTGTTGCTACCTTTTGGTCCTTTGGCAATTCTGCTACATTACTTAACGGGAAAGCATGGATGGGTCTTCTTTTTCACTTTACTTGGAATTACACCCTTGGCAGAACGTCTTGGTTATGCAACTGAGCAGCTTGCTTTCTACACTGGACCAACAGTTGGTGGTCTTTTGAATGCAACATTTGGAAATGCAACTGAAATGATCATATCAATATATGCGTTAAAGAGTGGGATGATTAGGGTTGTTCAACAGTCATTGCTGGGGTCAATCTTGTCAAATATGCTCCTTGTGCTTGGTTGCGCTTTTTTCACTGGTGGGATTATTCATCATACAAAGGTGCAGGTTTTCAATAAGGGTGCTGCTGTTGTAAATTCAGGATTGTTGTTGATGGCTGTAATGGGAACGACTTTTCCGGCCGTGCTTCATTTCACCCACACAGAACTTCATTTGGGAGAGTCAGCATTATCTCTTTCAAGATTTAGCAGTTGTATAATGTTAATTGCATATGCAAGCTACCTTTTCTTTCAACTAAAAAGTCAGCATGATCTCTATGGTCCTCTTGACGAGGAAGTTGATGGAGAAGTTGAGGACGATTCTGAGATTTATGCTTGGGAAGCAATTGGATGGCTTGCCATCTTGACTGTATGGGTATCTGTTTTATCTGGATACCTTGTTGATGCTATCCAGGGAGCATCTGAATCATTAAATTTGCCAGTGGCATTTATTAGTGTAATTTTGTTGCCAATTGTTGGAAATGCTGCTGAACATGCAAGTGCAATCATGTTTGCCATGAAGGATAAGCTTGACATCACTCTTGGAGTTGCTATTGGATCATCTACACAAATATCCATGTTTGTGATCCCGTTTTGCGTAGTAGTTGGATGGTGCATGGGAAAACCAATGGACTTAAACTTCAAACTCTTTGAAACAGCAACGTTGTTCATCACTGTGCTAGTAGTGGCCTTTATGTTGCAGGAGGGGACATCAAATTATTTCAAAGGTCTGATGCTTATTCTATGTTATCTCATAGTGGCAGCCAGTTTTTTTGTACACGTTGATCCTAGTAATGATGATTAA

Protein sequence

MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYASYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPSNDD*
Homology
BLAST of CSPI03G28780.1 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 587.8 bits (1514), Expect = 9.9e-167
Identity = 302/377 (80.11%), Postives = 340/377 (90.19%), Query Frame = 0

Query: 55  RPLRSAYIVLVRAKINVLLPFGPLAILLHYLTGKH-GWVFFFTLLGITPLAERLGYATEQ 114
           R L S  +V+++AKINVLLPFGPLA++LHYL+  H GWVF F+L+GITPLAERLGYATEQ
Sbjct: 59  RALTSVRVVILQAKINVLLPFGPLAVMLHYLSANHQGWVFLFSLIGITPLAERLGYATEQ 118

Query: 115 LAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFT 174
           LA YTGPT+GGLLNATFGNATEMIIS+YALK+GMIRVVQQSLLGSILSNMLLVLGCAFF 
Sbjct: 119 LALYTGPTIGGLLNATFGNATEMIISLYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFA 178

Query: 175 GGIIHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCI 234
           GG++H ++ QVFNK +AVVNSGLLLMAV+G  FPAVLHFTH+E+  G+S +SLSRFSSCI
Sbjct: 179 GGLVHPSRDQVFNKASAVVNSGLLLMAVLGLMFPAVLHFTHSEVQYGKSEVSLSRFSSCI 238

Query: 235 MLIAYASYLFFQLKSQHDLYGPL---DEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLS 294
           ML+AYASYLFFQLKSQ  LY P+   +EEV  + E++ EI   EAI WL +LT+W+S+LS
Sbjct: 239 MLVAYASYLFFQLKSQRSLYSPIGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILS 298

Query: 295 GYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQ 354
           GYLVDAIQGASESLN+PVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQ
Sbjct: 299 GYLVDAIQGASESLNMPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQ 358

Query: 355 ISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCY 414
           ISMFVIPFCVV+GW MG+ MDLNF+LFETATLFITVLVVAFMLQEGTSNYFKGLMLILCY
Sbjct: 359 ISMFVIPFCVVIGWIMGQQMDLNFQLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCY 418

Query: 415 LIVAASFFVHVDPSNDD 428
           LIVAASFFVHVDP + +
Sbjct: 419 LIVAASFFVHVDPDSSN 435

BLAST of CSPI03G28780.1 vs. ExPASy Swiss-Prot
Match: Q6K1C4 (Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX3 PE=2 SV=2)

HSP 1 Score: 564.3 bits (1453), Expect = 1.2e-159
Identity = 291/386 (75.39%), Postives = 330/386 (85.49%), Query Frame = 0

Query: 47  WKTSNIRIRPLRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAER 106
           W         LRS  IV+  +K+N+LLPFGP +I+LHY T +HG VF F++LGITPLAER
Sbjct: 31  WNLQRFVESALRSIRIVIFTSKLNLLLPFGPASIILHYTTSRHGLVFLFSMLGITPLAER 90

Query: 107 LGYATEQLAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLV 166
           LGYATEQLA YTGPTVGGLLNATFGNATEMII+IYALK+GMIRVVQQSLLGSILSNMLLV
Sbjct: 91  LGYATEQLAIYTGPTVGGLLNATFGNATEMIIAIYALKNGMIRVVQQSLLGSILSNMLLV 150

Query: 167 LGCAFFTGGIIHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSL 226
           +GCAFF GGI+H  K QVF+K  AVVNSGLLLMAVMG  FPAVLHFTH+E+  G S +SL
Sbjct: 151 MGCAFFAGGIVHRNKDQVFSKATAVVNSGLLLMAVMGLMFPAVLHFTHSEVRQGASEVSL 210

Query: 227 SRFSSCIMLIAYASYLFFQLKSQHDLYGPLDEEV-----DGEVEDDSEIYAWEAIGWLAI 286
           SRFSSCIML+AYASYL+FQL  +++ Y P+  E        E +++SEI  WE+I WLA+
Sbjct: 211 SRFSSCIMLVAYASYLYFQLSGRNNAYSPIGSEEMPNEDAAEEDEESEIGMWESIAWLAM 270

Query: 287 LTVWVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITL 346
           LT+WVS+LS YLV+AI+GAS+SLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITL
Sbjct: 271 LTLWVSILSEYLVNAIEGASDSLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITL 330

Query: 347 GVAIGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYF 406
           GVAIGSSTQISMFVIPFCVV+GW MG+ MDLNF+LFETATLFITVLVVAFMLQ+G +NY 
Sbjct: 331 GVAIGSSTQISMFVIPFCVVIGWMMGQKMDLNFQLFETATLFITVLVVAFMLQDGVANYL 390

Query: 407 KGLMLILCYLIVAASFFVHVDPSNDD 428
           KGLMLILCYLIVAASFFVHVDP + D
Sbjct: 391 KGLMLILCYLIVAASFFVHVDPQSSD 416

BLAST of CSPI03G28780.1 vs. ExPASy Swiss-Prot
Match: Q8L783 (Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 3.9e-155
Identity = 288/385 (74.81%), Postives = 325/385 (84.42%), Query Frame = 0

Query: 48  KTSNIRIRPLRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERL 107
           KT N  +  L+S  IV++  K+N+LLPFGPLAILLHYLT   GW+F  +L+GITPLAERL
Sbjct: 58  KTPNNSV--LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERL 117

Query: 108 GYATEQLAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVL 167
           GYATEQLA YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVL
Sbjct: 118 GYATEQLACYTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVL 177

Query: 168 GCAFFTGGIIHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLS 227
           GCAFF GG++   K QVF+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LS
Sbjct: 178 GCAFFCGGLVFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALS 237

Query: 228 RFSSCIMLIAYASYLFFQLKSQHDLYGPLDEEVD-----GEVEDDSEIYAWEAIGWLAIL 287
           RFSSCIML+AYA+YLFFQLKSQ   Y PL EE +      + ++D EI  WEAI WL+IL
Sbjct: 238 RFSSCIMLVAYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSIL 297

Query: 288 TVWVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLG 347
           T WVS+LSGYLVDAI+GAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LG
Sbjct: 298 TAWVSLLSGYLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLG 357

Query: 348 VAIGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFK 407
           VAIGSS QISMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFK
Sbjct: 358 VAIGSSIQISMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFK 417

Query: 408 GLMLILCYLIVAASFFVHVDPSNDD 428
           GLMLILCYLIVAASFFVH DP  DD
Sbjct: 418 GLMLILCYLIVAASFFVHEDPHQDD 440

BLAST of CSPI03G28780.1 vs. ExPASy Swiss-Prot
Match: Q39254 (Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 SV=2)

HSP 1 Score: 547.4 bits (1409), Expect = 1.5e-154
Identity = 284/375 (75.73%), Postives = 319/375 (85.07%), Query Frame = 0

Query: 57  LRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 116
           L S  IV+   K+N+LLPFGPLAIL+HY+    GWVF  TL+GITPLAERLGYATEQLA 
Sbjct: 65  LNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATEQLAC 124

Query: 117 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 176
           YTGPTVGGLLNATFGN TE+IISI+ALK+GMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 177 IHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLI 236
           + + K QVF+KG A VNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LSRFSSCIMLI
Sbjct: 185 VFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSCIMLI 244

Query: 237 AYASYLFFQLKSQHDLYGPLDEEVDGEVE-----DDSEIYAWEAIGWLAILTVWVSVLSG 296
           AYA+YLFFQLKSQ + Y PLDEE +   E     +D EI  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 297 YLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 356
           YLVDAI+GAS S N+P+AFIS ILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 357 SMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 416
           SMF +PFCVV+GW MG+ MDLNF+LFETA LFITV+VVAF LQEG+SNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLILCYL 424

Query: 417 IVAASFFVHVDPSND 427
           IVAASFFVH DP  D
Sbjct: 425 IVAASFFVHEDPHQD 439

BLAST of CSPI03G28780.1 vs. ExPASy Swiss-Prot
Match: Q9LFZ8 (Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=CAX6 PE=3 SV=3)

HSP 1 Score: 497.3 bits (1279), Expect = 1.8e-139
Identity = 264/371 (71.16%), Postives = 305/371 (82.21%), Query Frame = 0

Query: 57  LRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 116
           ++S  IV++  K+N+LLPFGPLAIL+HYLT   GW F  +L+GITPLAERLGYATEQL+ 
Sbjct: 76  VQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATEQLSC 135

Query: 117 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 176
           YTG TVGGLLNATFGN  E+IISI ALK+GMIRVVQ +LLGSILSN+LLVLGCAFF GG+
Sbjct: 136 YTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFFCGGL 195

Query: 177 IHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLI 236
           +   K QVF+K  AVV+SG+LLMAVMG  FP  LH+TH+E+H G S L+LSRF SCIML+
Sbjct: 196 VFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISCIMLV 255

Query: 237 AYASYLFFQLKSQHDLYGP---LDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYL 296
           AYA+YLFFQLKSQ   Y      +EE   + ++D EI  WEAI WL+I T WVS+LSGYL
Sbjct: 256 AYAAYLFFQLKSQPSFYTEKTNQNEETSND-DEDPEISKWEAIIWLSIFTAWVSLLSGYL 315

Query: 297 VDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISM 356
           VDAI+G S S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QISM
Sbjct: 316 VDAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISM 375

Query: 357 FVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQ-EGTSNYFKGLMLILCYLI 416
           F +PFCVV+GW MG  MDLN +LFETATL ITV+VVAF LQ EGTSNYFK LMLILCYLI
Sbjct: 376 FAVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLILCYLI 435

Query: 417 VAASFFVHVDP 424
           VAASFFVH DP
Sbjct: 436 VAASFFVHEDP 445

BLAST of CSPI03G28780.1 vs. ExPASy TrEMBL
Match: C8CBQ5 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX2 PE=2 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.5e-237
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA 60
           MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA 60

Query: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120
           YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP
Sbjct: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120

Query: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT 180
           TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT
Sbjct: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT 180

Query: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS 240
           KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS
Sbjct: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS 240

Query: 241 YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA 300
           YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA
Sbjct: 241 YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA 300

Query: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360
           SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV
Sbjct: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360

Query: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420
           VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH
Sbjct: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420

Query: 421 VDPSNDD 428
           VDPSNDD
Sbjct: 421 VDPSNDD 427

BLAST of CSPI03G28780.1 vs. ExPASy TrEMBL
Match: A0A1S3CKH8 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103501973 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.2e-234
Identity = 421/427 (98.59%), Postives = 425/427 (99.53%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA 60
           MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKT+NIRIRPLRSA
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTNNIRIRPLRSA 60

Query: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120
           YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP
Sbjct: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120

Query: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT 180
           TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 
Sbjct: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHK 180

Query: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS 240
           KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLIAYAS
Sbjct: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYAS 240

Query: 241 YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA 300
           YLFFQLKSQH+LYGPLDEEVDGEVEDDSEI+AWEAIGWLAILTVWVS+LSGYLVDAIQGA
Sbjct: 241 YLFFQLKSQHNLYGPLDEEVDGEVEDDSEIFAWEAIGWLAILTVWVSILSGYLVDAIQGA 300

Query: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360
           SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV
Sbjct: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360

Query: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420
           VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH
Sbjct: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420

Query: 421 VDPSNDD 428
           VDPSNDD
Sbjct: 421 VDPSNDD 427

BLAST of CSPI03G28780.1 vs. ExPASy TrEMBL
Match: A0A6J1IRU5 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111479941 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.5e-223
Identity = 405/428 (94.63%), Postives = 415/428 (96.96%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 60
           MGSTEEKTNYLSDE  PLKSSPVAKNV HSFDCET  VRSSPD TRWWKT+NIRIRPLRS
Sbjct: 1   MGSTEEKTNYLSDEGTPLKSSPVAKNVSHSFDCETGPVRSSPDVTRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGI PLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240
            KVQVFNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLIAYA
Sbjct: 181 -KVQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQ DLYGPLDE  + EVEDDSEIYAWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQQDLYGPLDEVGEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKG+MLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGVMLILCYLIVAASFFV 420

Query: 421 HVDPSNDD 428
           H+DP+ND+
Sbjct: 421 HIDPTNDN 427

BLAST of CSPI03G28780.1 vs. ExPASy TrEMBL
Match: A0A6J1GWA3 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111457727 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.9e-223
Identity = 405/428 (94.63%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 60
           MGSTEEKTNYLSDE  PLKSSPVAKNV HSFDCET  V SSPD TRWWKT+NIRIRPLRS
Sbjct: 1   MGSTEEKTNYLSDEGTPLKSSPVAKNVSHSFDCETGPVHSSPDVTRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGI PLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240
            KVQVFNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLIAYA
Sbjct: 181 -KVQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQ DLYGPLDE  + EVEDDSEIYAWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQQDLYGPLDEVGEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNDD 428
           H+DP+ND+
Sbjct: 421 HIDPTNDN 427

BLAST of CSPI03G28780.1 vs. ExPASy TrEMBL
Match: A0A6J1DGR6 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111020296 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 6.5e-222
Identity = 403/428 (94.16%), Postives = 411/428 (96.03%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 60
           MGS EEKTNYLSDEEIP +SSPVAKNVLHSFD ETA VRSSPD + WW  +NIRIRPL+S
Sbjct: 1   MGSIEEKTNYLSDEEIPFRSSPVAKNVLHSFDFETAHVRSSPDASTWWNINNIRIRPLKS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180

Query: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240
            KVQ FNKG AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 KKVQTFNKGTAVVNSGLLLMAVMGTTFPAVLHFTHTELHFGESALSLSRFSSCIMLVAYA 240

Query: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQH+LYGPLDEEVD    D SE++AWEAIGWLAILTVWVSVLSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQHNLYGPLDEEVDDGDADASEMFAWEAIGWLAILTVWVSVLSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNDD 428
           HVDPSNDD
Sbjct: 421 HVDPSNDD 428

BLAST of CSPI03G28780.1 vs. NCBI nr
Match: NP_001267651.1 (vacuolar cation/proton exchanger 2-like [Cucumis sativus] >XP_011650230.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >XP_011650232.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >XP_011650233.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >XP_031737456.1 vacuolar cation/proton exchanger 2-like isoform X1 [Cucumis sativus] >ACU82368.1 cation exchanger 2 [Cucumis sativus] >KGN58485.1 hypothetical protein Csa_001856 [Cucumis sativus])

HSP 1 Score: 831.6 bits (2147), Expect = 3.0e-237
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA 60
           MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA 60

Query: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120
           YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP
Sbjct: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120

Query: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT 180
           TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT
Sbjct: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT 180

Query: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS 240
           KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS
Sbjct: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS 240

Query: 241 YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA 300
           YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA
Sbjct: 241 YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA 300

Query: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360
           SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV
Sbjct: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360

Query: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420
           VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH
Sbjct: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420

Query: 421 VDPSNDD 428
           VDPSNDD
Sbjct: 421 VDPSNDD 427

BLAST of CSPI03G28780.1 vs. NCBI nr
Match: XP_008463979.1 (PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463980.1 PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463981.1 PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo])

HSP 1 Score: 822.0 bits (2122), Expect = 2.4e-234
Identity = 421/427 (98.59%), Postives = 425/427 (99.53%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTSNIRIRPLRSA 60
           MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKT+NIRIRPLRSA
Sbjct: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPDTRWWKTNNIRIRPLRSA 60

Query: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120
           YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP
Sbjct: 61  YIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 120

Query: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHT 180
           TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 
Sbjct: 121 TVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHHK 180

Query: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYAS 240
           KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLIAYAS
Sbjct: 181 KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYAS 240

Query: 241 YLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQGA 300
           YLFFQLKSQH+LYGPLDEEVDGEVEDDSEI+AWEAIGWLAILTVWVS+LSGYLVDAIQGA
Sbjct: 241 YLFFQLKSQHNLYGPLDEEVDGEVEDDSEIFAWEAIGWLAILTVWVSILSGYLVDAIQGA 300

Query: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360
           SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV
Sbjct: 301 SESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCV 360

Query: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420
           VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH
Sbjct: 361 VVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVH 420

Query: 421 VDPSNDD 428
           VDPSNDD
Sbjct: 421 VDPSNDD 427

BLAST of CSPI03G28780.1 vs. NCBI nr
Match: XP_038902338.1 (vacuolar cation/proton exchanger 3-like [Benincasa hispida])

HSP 1 Score: 788.9 bits (2036), Expect = 2.2e-224
Identity = 411/430 (95.58%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSP-DTRWWKTSNIRIRPLRS 60
           MGSTEEKTNYLSDEEI LKSSPVAKNVLHSFDCETA VRSSP D RWWKT+NIRIRPLRS
Sbjct: 1   MGSTEEKTNYLSDEEILLKSSPVAKNVLHSFDCETAPVRSSPDDKRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHG VFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGLVFFFTLLGITPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240
            KVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIML+AYA
Sbjct: 181 KKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLVAYA 240

Query: 241 SYLFFQLKSQHDLYGPLD--EEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAI 300
           SYLFFQLKSQH+LYGPLD   EV+ E EDDSEIYAWEAIGWLAILTVWVS+LSGYLVDAI
Sbjct: 241 SYLFFQLKSQHNLYGPLDGEVEVEDEDEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAI 300

Query: 301 QGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIP 360
           QGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIP
Sbjct: 301 QGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIP 360

Query: 361 FCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASF 420
           FCVVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASF
Sbjct: 361 FCVVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASF 420

Query: 421 FVHVDPSNDD 428
           FVHVDPSNDD
Sbjct: 421 FVHVDPSNDD 430

BLAST of CSPI03G28780.1 vs. NCBI nr
Match: KAG6581803.1 (Vacuolar cation/proton exchanger 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 787.3 bits (2032), Expect = 6.5e-224
Identity = 407/428 (95.09%), Postives = 416/428 (97.20%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 60
           MGSTEEKTNYLSDE  PLKSSPVAKNVLHSFDCET  VRSSPD TRWWKT+NIRIRPLRS
Sbjct: 71  MGSTEEKTNYLSDEGTPLKSSPVAKNVLHSFDCETGPVRSSPDVTRWWKTNNIRIRPLRS 130

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGI PLAERLGYATEQLAFYTG
Sbjct: 131 AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 190

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 191 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 250

Query: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240
            KVQVFNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLIAYA
Sbjct: 251 -KVQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 310

Query: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQ DLYGPLDE  + EVEDDSEIYAWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 311 SYLFFQLKSQQDLYGPLDEVGEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 370

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 371 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 430

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 431 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 490

Query: 421 HVDPSNDD 428
           H+DP+ND+
Sbjct: 491 HIDPTNDN 497

BLAST of CSPI03G28780.1 vs. NCBI nr
Match: XP_023526825.1 (vacuolar cation/proton exchanger 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.8 bits (2028), Expect = 1.9e-223
Identity = 406/428 (94.86%), Postives = 415/428 (96.96%), Query Frame = 0

Query: 1   MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 60
           MGSTEEKTNYLSDE  PLKS PVAKNV HSFDCET  VRSSPD TRWWKT+NIRIRPLRS
Sbjct: 1   MGSTEEKTNYLSDEGTPLKSPPVAKNVSHSFDCETGPVRSSPDVTRWWKTNNIRIRPLRS 60

Query: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 120
           AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGI PLAERLGYATEQLAFYTG
Sbjct: 61  AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGIAPLAERLGYATEQLAFYTG 120

Query: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 180
           PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI+HH
Sbjct: 121 PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIVHH 180

Query: 181 TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 240
            KVQVFNKG+AVVNSGLLLMAVMGTTFPAVLHFTHTELH GESALSLSRFSSCIMLIAYA
Sbjct: 181 -KVQVFNKGSAVVNSGLLLMAVMGTTFPAVLHFTHTELHSGESALSLSRFSSCIMLIAYA 240

Query: 241 SYLFFQLKSQHDLYGPLDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLVDAIQG 300
           SYLFFQLKSQ DLYGPLDE V+ EVEDDSEIYAWEAIGWLAILTVWVS+LSGYLVDAIQG
Sbjct: 241 SYLFFQLKSQQDLYGPLDEVVEDEVEDDSEIYAWEAIGWLAILTVWVSILSGYLVDAIQG 300

Query: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360
           ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC
Sbjct: 301 ASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFC 360

Query: 361 VVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420
           VVVGWCMG+PMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV
Sbjct: 361 VVVGWCMGEPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFV 420

Query: 421 HVDPSNDD 428
           H+DP+ND+
Sbjct: 421 HIDPTNDN 427

BLAST of CSPI03G28780.1 vs. TAIR 10
Match: AT1G55730.1 (cation exchanger 5 )

HSP 1 Score: 549.3 bits (1414), Expect = 2.8e-156
Identity = 288/385 (74.81%), Postives = 325/385 (84.42%), Query Frame = 0

Query: 48  KTSNIRIRPLRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERL 107
           KT N  +  L+S  IV++  K+N+LLPFGPLAILLHYLT   GW+F  +L+GITPLAERL
Sbjct: 58  KTPNNSV--LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERL 117

Query: 108 GYATEQLAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVL 167
           GYATEQLA YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVL
Sbjct: 118 GYATEQLACYTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVL 177

Query: 168 GCAFFTGGIIHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLS 227
           GCAFF GG++   K QVF+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LS
Sbjct: 178 GCAFFCGGLVFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALS 237

Query: 228 RFSSCIMLIAYASYLFFQLKSQHDLYGPLDEEVD-----GEVEDDSEIYAWEAIGWLAIL 287
           RFSSCIML+AYA+YLFFQLKSQ   Y PL EE +      + ++D EI  WEAI WL+IL
Sbjct: 238 RFSSCIMLVAYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSIL 297

Query: 288 TVWVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLG 347
           T WVS+LSGYLVDAI+GAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LG
Sbjct: 298 TAWVSLLSGYLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLG 357

Query: 348 VAIGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFK 407
           VAIGSS QISMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFK
Sbjct: 358 VAIGSSIQISMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFK 417

Query: 408 GLMLILCYLIVAASFFVHVDPSNDD 428
           GLMLILCYLIVAASFFVH DP  DD
Sbjct: 418 GLMLILCYLIVAASFFVHEDPHQDD 440

BLAST of CSPI03G28780.1 vs. TAIR 10
Match: AT1G55730.2 (cation exchanger 5 )

HSP 1 Score: 549.3 bits (1414), Expect = 2.8e-156
Identity = 288/385 (74.81%), Postives = 325/385 (84.42%), Query Frame = 0

Query: 48  KTSNIRIRPLRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERL 107
           KT N  +  L+S  IV++  K+N+LLPFGPLAILLHYLT   GW+F  +L+GITPLAERL
Sbjct: 58  KTPNNSV--LQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERL 117

Query: 108 GYATEQLAFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVL 167
           GYATEQLA YTG TVGGLLNATFGN TE+IISI+ALKSGMIRVVQ +LLGSILSNMLLVL
Sbjct: 118 GYATEQLACYTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVL 177

Query: 168 GCAFFTGGIIHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLS 227
           GCAFF GG++   K QVF+KG AVVNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LS
Sbjct: 178 GCAFFCGGLVFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALS 237

Query: 228 RFSSCIMLIAYASYLFFQLKSQHDLYGPLDEEVD-----GEVEDDSEIYAWEAIGWLAIL 287
           RFSSCIML+AYA+YLFFQLKSQ   Y PL EE +      + ++D EI  WEAI WL+IL
Sbjct: 238 RFSSCIMLVAYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSIL 297

Query: 288 TVWVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLG 347
           T WVS+LSGYLVDAI+GAS S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LG
Sbjct: 298 TAWVSLLSGYLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLG 357

Query: 348 VAIGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFK 407
           VAIGSS QISMF +PFCVV+GW MG  MDLNF+LFETATLFITV+VVAF LQEGTSNYFK
Sbjct: 358 VAIGSSIQISMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFK 417

Query: 408 GLMLILCYLIVAASFFVHVDPSNDD 428
           GLMLILCYLIVAASFFVH DP  DD
Sbjct: 418 GLMLILCYLIVAASFFVHEDPHQDD 440

BLAST of CSPI03G28780.1 vs. TAIR 10
Match: AT3G13320.1 (cation exchanger 2 )

HSP 1 Score: 547.4 bits (1409), Expect = 1.1e-155
Identity = 284/375 (75.73%), Postives = 319/375 (85.07%), Query Frame = 0

Query: 57  LRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 116
           L S  IV+   K+N+LLPFGPLAIL+HY+    GWVF  TL+GITPLAERLGYATEQLA 
Sbjct: 65  LNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATEQLAC 124

Query: 117 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 176
           YTGPTVGGLLNATFGN TE+IISI+ALK+GMIRVVQ +LLGSILSNMLLVLGCAFF GG+
Sbjct: 125 YTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFFCGGL 184

Query: 177 IHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLI 236
           + + K QVF+KG A VNSGLLLMAVMG  FPAVLH+TH+E+H G S L+LSRFSSCIMLI
Sbjct: 185 VFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSCIMLI 244

Query: 237 AYASYLFFQLKSQHDLYGPLDEEVDGEVE-----DDSEIYAWEAIGWLAILTVWVSVLSG 296
           AYA+YLFFQLKSQ + Y PLDEE +   E     +D EI  WEAI WL+ILT WVS+LSG
Sbjct: 245 AYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVSLLSG 304

Query: 297 YLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 356
           YLVDAI+GAS S N+P+AFIS ILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QI
Sbjct: 305 YLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQI 364

Query: 357 SMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYL 416
           SMF +PFCVV+GW MG+ MDLNF+LFETA LFITV+VVAF LQEG+SNYFKGLMLILCYL
Sbjct: 365 SMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLILCYL 424

Query: 417 IVAASFFVHVDPSND 427
           IVAASFFVH DP  D
Sbjct: 425 IVAASFFVHEDPHQD 439

BLAST of CSPI03G28780.1 vs. TAIR 10
Match: AT1G55720.1 (cation exchanger 6 )

HSP 1 Score: 497.3 bits (1279), Expect = 1.2e-140
Identity = 264/371 (71.16%), Postives = 305/371 (82.21%), Query Frame = 0

Query: 57  LRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAF 116
           ++S  IV++  K+N+LLPFGPLAIL+HYLT   GW F  +L+GITPLAERLGYATEQL+ 
Sbjct: 76  VQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATEQLSC 135

Query: 117 YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 176
           YTG TVGGLLNATFGN  E+IISI ALK+GMIRVVQ +LLGSILSN+LLVLGCAFF GG+
Sbjct: 136 YTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFFCGGL 195

Query: 177 IHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLI 236
           +   K QVF+K  AVV+SG+LLMAVMG  FP  LH+TH+E+H G S L+LSRF SCIML+
Sbjct: 196 VFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISCIMLV 255

Query: 237 AYASYLFFQLKSQHDLYGP---LDEEVDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYL 296
           AYA+YLFFQLKSQ   Y      +EE   + ++D EI  WEAI WL+I T WVS+LSGYL
Sbjct: 256 AYAAYLFFQLKSQPSFYTEKTNQNEETSND-DEDPEISKWEAIIWLSIFTAWVSLLSGYL 315

Query: 297 VDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISM 356
           VDAI+G S S  +P++FISVILLPIVGNAAEHA AIMFAMKDKLD++LGVAIGSS QISM
Sbjct: 316 VDAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISM 375

Query: 357 FVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQ-EGTSNYFKGLMLILCYLI 416
           F +PFCVV+GW MG  MDLN +LFETATL ITV+VVAF LQ EGTSNYFK LMLILCYLI
Sbjct: 376 FAVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLILCYLI 435

Query: 417 VAASFFVHVDP 424
           VAASFFVH DP
Sbjct: 436 VAASFFVHEDP 445

BLAST of CSPI03G28780.1 vs. TAIR 10
Match: AT5G01490.1 (cation exchanger 4 )

HSP 1 Score: 345.5 bits (885), Expect = 6.1e-95
Identity = 192/382 (50.26%), Postives = 260/382 (68.06%), Query Frame = 0

Query: 55  RPLRSAYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQL 114
           R L +   VL+  K+ +L P  PLA++ H       WVF  +LLG+TPLAER+ + TEQ+
Sbjct: 62  RILTNLQEVLLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQI 121

Query: 115 AFYTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTG 174
           AF+TGPTVGGL+NAT GNATEMII+I A+    +R+V+ SLLGSILSN+L VLG + F G
Sbjct: 122 AFHTGPTVGGLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLG 181

Query: 175 GIIHHTKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFT-HTELHLGESALSLSRFSSCI 234
           GI +  K Q F+     +NS LL +A++  T P ++ FT   E + G   + LSR SS +
Sbjct: 182 GISNLRKHQSFDPRQGDMNSMLLYLALLCQTLPMIMRFTMEAEEYDGSDVVVLSRASSFV 241

Query: 235 MLIAYASYLFFQLKSQHDLYGP---------LDEEVDGEVEDDSEIYAWEAIGWLAILTV 294
           MLIAY ++L F L S H    P          D++V  + E+ + I  W AI WL I+T+
Sbjct: 242 MLIAYLAFLIFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMTL 301

Query: 295 WVSVLSGYLVDAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVA 354
            V++LS YLV  IQ A++S  L V FI +ILLPIVGNAAEHA A++FA ++KLDITLG+A
Sbjct: 302 LVALLSDYLVSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGIA 361

Query: 355 IGSSTQISMFVIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGL 414
           +GS+TQI++FV+P  V+V W MG  MDLNF L ETA   +++LV + +LQ+GTSNY KGL
Sbjct: 362 LGSATQIALFVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKGL 421

Query: 415 MLILCYLIVAASFFVHVDPSND 427
           +L+LCY+++AA FFV   PS +
Sbjct: 422 VLLLCYVVIAACFFVSNSPSTE 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5KQN09.9e-16780.11Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q6K1C41.2e-15975.39Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q8L7833.9e-15574.81Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 ... [more]
Q392541.5e-15475.73Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 ... [more]
Q9LFZ81.8e-13971.16Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
C8CBQ51.5e-237100.00Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX2 PE=2 SV=1[more]
A0A1S3CKH81.2e-23498.59Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103501973 PE=3 SV... [more]
A0A6J1IRU53.5e-22394.63Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111479941 PE=... [more]
A0A6J1GWA35.9e-22394.63Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111457727 P... [more]
A0A6J1DGR66.5e-22294.16Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111020296 ... [more]
Match NameE-valueIdentityDescription
NP_001267651.13.0e-237100.00vacuolar cation/proton exchanger 2-like [Cucumis sativus] >XP_011650230.1 vacuol... [more]
XP_008463979.12.4e-23498.59PREDICTED: vacuolar cation/proton exchanger 3-like [Cucumis melo] >XP_008463980.... [more]
XP_038902338.12.2e-22495.58vacuolar cation/proton exchanger 3-like [Benincasa hispida][more]
KAG6581803.16.5e-22495.09Vacuolar cation/proton exchanger 2, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023526825.11.9e-22394.86vacuolar cation/proton exchanger 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G55730.12.8e-15674.81cation exchanger 5 [more]
AT1G55730.22.8e-15674.81cation exchanger 5 [more]
AT3G13320.11.1e-15575.73cation exchanger 2 [more]
AT1G55720.11.2e-14071.16cation exchanger 6 [more]
AT5G01490.16.1e-9550.26cation exchanger 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTIGRFAMTIGR00846TIGR00846coord: 63..418
e-value: 4.0E-115
score: 382.5
NoneNo IPR availablePANTHERPTHR31503:SF54VACUOLAR CATION/PROTON EXCHANGERcoord: 1..426
IPR004798Calcium/proton exchanger CAX-likeTIGRFAMTIGR00378TIGR00378coord: 72..418
e-value: 5.6E-102
score: 339.5
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 91..246
e-value: 1.1E-19
score: 70.9
coord: 276..415
e-value: 2.9E-19
score: 69.5
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 120..419
e-value: 4.0E-30
score: 106.9
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 1..426

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI03G28780CSPI03G28780gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G28780.1.utr3p1CSPI03G28780.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G28780.1.cds12CSPI03G28780.1.cds12CDS
CSPI03G28780.1.cds11CSPI03G28780.1.cds11CDS
CSPI03G28780.1.cds10CSPI03G28780.1.cds10CDS
CSPI03G28780.1.cds9CSPI03G28780.1.cds9CDS
CSPI03G28780.1.cds8CSPI03G28780.1.cds8CDS
CSPI03G28780.1.cds7CSPI03G28780.1.cds7CDS
CSPI03G28780.1.cds6CSPI03G28780.1.cds6CDS
CSPI03G28780.1.cds5CSPI03G28780.1.cds5CDS
CSPI03G28780.1.cds4CSPI03G28780.1.cds4CDS
CSPI03G28780.1.cds3CSPI03G28780.1.cds3CDS
CSPI03G28780.1.cds2CSPI03G28780.1.cds2CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G28780.1.utr5p1CSPI03G28780.1.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI03G28780.1CSPI03G28780.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006816 calcium ion transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity