CSPI03G27520.1 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI03G27520.1
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type domain-containing protein
LocationChr3: 25103033 .. 25104848 (+)
Sequence length1816
RNA-Seq ExpressionCSPI03G27520.1
SyntenyCSPI03G27520.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGTAATTGATCCAATAAAACAGGGGAATTCATCTGAAATACCTGAATATCTTCTTCTTCTTGCCGACCTCCAAAGTTCTTGAAGAGCCCATTTCCTGCCAAATTTACATTATATTGGATCCATTGTTCGCTTACTATCCTAAGGTGGGTTTGTTTTATTTTTTGCAATTTCCAAATTGTTTGATTCCACAAGATAAAGAGAAAATAGATGATTAATGCACGAACCTCCTGAACCTTAAATCTATTTTTTTTTTGTTTTTTTTTATTAAGACGATCATCTATTTGTATCAGTAATTGATCGAAAAACCATGGGGATTCATCTGAAACACTTAAATTTCTTCTTCTTTTTCTTCTTCTTCCTGCTAAGATCAAACTTCTTACAGAAACCATTCCTGTTACACTGTCAATTGGATCTCTCAGTTAACAATATCCCAAAATGAATTAATGTCAGAAAATAATGCATGAATTCGTGAAGCTTAATTTGATTGTGTTTTTTATGACCATCAATTTACATCAATAATTAGGTTTTTACTGGAAAAGCAATGATCTATTGATTTCTACTAACCTGCATTCAAATTCGATCACCTTTTGACATTTCTTCTTCTTTATGTGATCTGCACGATTCGTGAACACGGAAGGTTGTTTCGATCCTCAAATTTCCGATCAAATGTCGATGGTTGAGGATGGCCGTAGCCTGGCAGGCTATACACTGGATGATGTTTTGGCTAACAATAGATTAGGGCCACCGGCCACTGCAAGGGCCACCGAAATCCGCGGCCGAACCCTACTTGACATAATCCGTGACGAGGAGCCGAGCACCGGCAAGGGGCTTTTCGGAAAGGATAAAAGGACTTGGAAGTCTTTTAGGGATAAGCTCCGGCTGAAGCGCGCCGGCTCTGCATGGGTCTCAACGGTTCCAATTCCGACCTCTGATATTCCTGTTCACAACAAGCGCTCTCTAATGGGAAGACGAAATCAAGTCCGTTTTAACACCGGTTCGTCCAAAAACTCCCCCGATAATCAATCTCGTCATCCGTCCGAAGAAACTTCCAAATCCACTCGCCGTCAAATGTGTCGACAAACTTCCACAATGAACAGCTCAAGCGATTCCTCTGCGTTTCATTATGATGAATCCTCCGAGTACTCATCGTCTCCAGATGCAGCGCCAGTTCGAATCATGAGACCGCAAATGTCACGCCATAACTCAGTCCGTGTCTCAAATCCAATGGAGAATTACACGGATCCAACAGAACCAGATCGCGATAACCGAATCCGACACTTGAATTTATCAGAACACAGAATGATGTCCGCAAGAGAAGCCGTCGCAGCACAAGAAGCAGCAGATGCAGCTTCCGCCGCTGCCGCAGCAGCAGCAGCAGCGGAAGCCGCAGAAGAAGAAAAAAAACAAAACGAAAACAACAACGAGAGCGGAAATGAAAACGAAGAGGAAGAATCAACAACGGAATCAGAGGAGGAGGAGGAGGAGTCTTCGCCAGCGTCGGAACCGGTGAGGATGTCGTTGATGGATCTATTGCATGAGACGGACAGGGAAATGGGTTTTGAAAGATCGAGTTACGGAATGGGATTTGAAGGAAATTTCTTAGAGGAAGATGAAGATGATGAGTACGAAGAAGATGAAGATGATGGTAATGGTGAAGAATTCAGCTGTTGCGTTTGTATGGTGAAACATAAGAACGGGCCGTTGGCGTCTTGTGGGCATACTTTTTGCCGGCTGTGTTCGAAGGAGTTGATGGTAAGCCGCGGGAACTGTCCTACTTGCAGTAATTTCATCTTGGAAATCCTTGATGCCTTTTGA

mRNA sequence

CAGTAATTGATCCAATAAAACAGGGGAATTCATCTGAAATACCTGAATATCTTCTTCTTCTTGCCGACCTCCAAAGTTCTTGAAGAGCCCATTTCCTGCCAAATTTACATTATATTGGATCCATTGTTCGCTTACTATCCTAAGGTGGGTTTGTTTTATTTTTTGCAATTTCCAAATTGTTTGATTCCACAAGATAAAGAGAAAATAGATGATTAATGCACGAACCTCCTGAACCTTAAATCTATTTTTTTTTTGTTTTTTTTTATTAAGACGATCATCTATTTGTATCAGTAATTGATCGAAAAACCATGGGGATTCATCTGAAACACTTAAATTTCTTCTTCTTTTTCTTCTTCTTCCTGCTAAGATCAAACTTCTTACAGAAACCATTCCTGTTACACTGTCAATTGGATCTCTCAGTTAACAATATCCCAAAATGAATTAATGTCAGAAAATAATGCATGAATTCGTGAAGCTTAATTTGATTGTGTTTTTTATGACCATCAATTTACATCAATAATTAGGTTTTTACTGGAAAAGCAATGATCTATTGATTTCTACTAACCTGCATTCAAATTCGATCACCTTTTGACATTTCTTCTTCTTTATGTGATCTGCACGATTCGTGAACACGGAAGGTTGTTTCGATCCTCAAATTTCCGATCAAATGTCGATGGTTGAGGATGGCCGTAGCCTGGCAGGCTATACACTGGATGATGTTTTGGCTAACAATAGATTAGGGCCACCGGCCACTGCAAGGGCCACCGAAATCCGCGGCCGAACCCTACTTGACATAATCCGTGACGAGGAGCCGAGCACCGGCAAGGGGCTTTTCGGAAAGGATAAAAGGACTTGGAAGTCTTTTAGGGATAAGCTCCGGCTGAAGCGCGCCGGCTCTGCATGGGTCTCAACGGTTCCAATTCCGACCTCTGATATTCCTGTTCACAACAAGCGCTCTCTAATGGGAAGACGAAATCAAGTCCGTTTTAACACCGGTTCGTCCAAAAACTCCCCCGATAATCAATCTCGTCATCCGTCCGAAGAAACTTCCAAATCCACTCGCCGTCAAATGTGTCGACAAACTTCCACAATGAACAGCTCAAGCGATTCCTCTGCGTTTCATTATGATGAATCCTCCGAGTACTCATCGTCTCCAGATGCAGCGCCAGTTCGAATCATGAGACCGCAAATGTCACGCCATAACTCAGTCCGTGTCTCAAATCCAATGGAGAATTACACGGATCCAACAGAACCAGATCGCGATAACCGAATCCGACACTTGAATTTATCAGAACACAGAATGATGTCCGCAAGAGAAGCCGTCGCAGCACAAGAAGCAGCAGATGCAGCTTCCGCCGCTGCCGCAGCAGCAGCAGCAGCGGAAGCCGCAGAAGAAGAAAAAAAACAAAACGAAAACAACAACGAGAGCGGAAATGAAAACGAAGAGGAAGAATCAACAACGGAATCAGAGGAGGAGGAGGAGGAGTCTTCGCCAGCGTCGGAACCGGTGAGGATGTCGTTGATGGATCTATTGCATGAGACGGACAGGGAAATGGGTTTTGAAAGATCGAGTTACGGAATGGGATTTGAAGGAAATTTCTTAGAGGAAGATGAAGATGATGAGTACGAAGAAGATGAAGATGATGGTAATGGTGAAGAATTCAGCTGTTGCGTTTGTATGGTGAAACATAAGAACGGGCCGTTGGCGTCTTGTGGGCATACTTTTTGCCGGCTGTGTTCGAAGGAGTTGATGGTAAGCCGCGGGAACTGTCCTACTTGCAGTAATTTCATCTTGGAAATCCTTGATGCCTTTTGA

Coding sequence (CDS)

ATGTCGATGGTTGAGGATGGCCGTAGCCTGGCAGGCTATACACTGGATGATGTTTTGGCTAACAATAGATTAGGGCCACCGGCCACTGCAAGGGCCACCGAAATCCGCGGCCGAACCCTACTTGACATAATCCGTGACGAGGAGCCGAGCACCGGCAAGGGGCTTTTCGGAAAGGATAAAAGGACTTGGAAGTCTTTTAGGGATAAGCTCCGGCTGAAGCGCGCCGGCTCTGCATGGGTCTCAACGGTTCCAATTCCGACCTCTGATATTCCTGTTCACAACAAGCGCTCTCTAATGGGAAGACGAAATCAAGTCCGTTTTAACACCGGTTCGTCCAAAAACTCCCCCGATAATCAATCTCGTCATCCGTCCGAAGAAACTTCCAAATCCACTCGCCGTCAAATGTGTCGACAAACTTCCACAATGAACAGCTCAAGCGATTCCTCTGCGTTTCATTATGATGAATCCTCCGAGTACTCATCGTCTCCAGATGCAGCGCCAGTTCGAATCATGAGACCGCAAATGTCACGCCATAACTCAGTCCGTGTCTCAAATCCAATGGAGAATTACACGGATCCAACAGAACCAGATCGCGATAACCGAATCCGACACTTGAATTTATCAGAACACAGAATGATGTCCGCAAGAGAAGCCGTCGCAGCACAAGAAGCAGCAGATGCAGCTTCCGCCGCTGCCGCAGCAGCAGCAGCAGCGGAAGCCGCAGAAGAAGAAAAAAAACAAAACGAAAACAACAACGAGAGCGGAAATGAAAACGAAGAGGAAGAATCAACAACGGAATCAGAGGAGGAGGAGGAGGAGTCTTCGCCAGCGTCGGAACCGGTGAGGATGTCGTTGATGGATCTATTGCATGAGACGGACAGGGAAATGGGTTTTGAAAGATCGAGTTACGGAATGGGATTTGAAGGAAATTTCTTAGAGGAAGATGAAGATGATGAGTACGAAGAAGATGAAGATGATGGTAATGGTGAAGAATTCAGCTGTTGCGTTTGTATGGTGAAACATAAGAACGGGCCGTTGGCGTCTTGTGGGCATACTTTTTGCCGGCTGTGTTCGAAGGAGTTGATGGTAAGCCGCGGGAACTGTCCTACTTGCAGTAATTTCATCTTGGAAATCCTTGATGCCTTTTGA

Protein sequence

MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNSVRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKELMVSRGNCPTCSNFILEILDAF*
Homology
BLAST of CSPI03G27520.1 vs. ExPASy TrEMBL
Match: A0A0A0L944 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G628230 PE=4 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.1e-203
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS
Sbjct: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240
           VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240

Query: 241 AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER 300
           AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER
Sbjct: 241 AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER 300

Query: 301 SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE 360
           SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE
Sbjct: 301 SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE 360

Query: 361 LMVSRGNCPTCSNFILEILDAF 383
           LMVSRGNCPTCSNFILEILDAF
Sbjct: 361 LMVSRGNCPTCSNFILEILDAF 382

BLAST of CSPI03G27520.1 vs. ExPASy TrEMBL
Match: A0A5D3E0G9 (E3 ubiquitin-protein ligase cblA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold880G00010 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.2e-185
Identity = 358/383 (93.47%), Postives = 366/383 (95.56%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPS GKGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPV  KRSL+GRRNQ+RFNTGSSKNS DN S
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           RHPSEE+SKSTRRQM RQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAAS-AAAAAAAAAE 240
           VRVSNPMENYTDPTEPDRDNRIRH NLS+HRMMSAREAVAAQEAADAAS AAAAAAAAAE
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHSNLSDHRMMSAREAVAAQEAADAASAAAAAAAAAAE 240

Query: 241 AAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFE 300
           AAEEEKKQNENNN +GNENE+EESTT S E EEESSPASEPVRMSLMDLLHETDREMGFE
Sbjct: 241 AAEEEKKQNENNNLNGNENEKEESTTGS-EGEEESSPASEPVRMSLMDLLHETDREMGFE 300

Query: 301 RSSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSK 360
           RSSYGMGFE NF E+DEDDEYEEDED+GNG EFSCCVCMVKHKN PLA CGHTFCRLCSK
Sbjct: 301 RSSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCRLCSK 360

Query: 361 ELMVSRGNCPTCSNFILEILDAF 383
           ELMVSRGNCPTCSNFILEILDAF
Sbjct: 361 ELMVSRGNCPTCSNFILEILDAF 382

BLAST of CSPI03G27520.1 vs. ExPASy TrEMBL
Match: A0A1S3CNY7 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase cblA-like OS=Cucumis melo OX=3656 GN=LOC103503074 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.3e-184
Identity = 352/382 (92.15%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPS GKGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPV  KRSL+GRRNQ+RFNTGSSKNS DN S
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           RHPSEE+SKSTRRQM RQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240
           VRVSNPMENYTDPTEPDRDNRIRH NLS+HRMMSAREAVAAQEAADAASAAAAAAAAA +
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHSNLSDHRMMSAREAVAAQEAADAASAAAAAAAAAGS 240

Query: 241 AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER 300
           +   KKQNENNN +GNENE+EESTT S E EEESSPASEPVRMSLMDLLHETDREMGFER
Sbjct: 241 SRRRKKQNENNNLNGNENEKEESTTGS-EGEEESSPASEPVRMSLMDLLHETDREMGFER 300

Query: 301 SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE 360
           SSYGMGFE NF E+DEDDEYEEDED+GNG EFSCCVCMVKHKN PLA CGHTFCRLCSKE
Sbjct: 301 SSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCRLCSKE 360

Query: 361 LMVSRGNCPTCSNFILEILDAF 383
           LMVSRGNCPTCSNFILEILDAF
Sbjct: 361 LMVSRGNCPTCSNFILEILDAF 381

BLAST of CSPI03G27520.1 vs. ExPASy TrEMBL
Match: A0A6J1K5D6 (E3 ubiquitin-protein ligase rnf8-A-like OS=Cucurbita maxima OX=3661 GN=LOC111491321 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.3e-144
Identity = 292/386 (75.65%), Postives = 332/386 (86.01%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPP--ATARATEIRGRTLLDIIRDEEPSTGKGLFGK 60
           MS++E+GRSLAGYTLDDVLANNR GPP  ATARA EIRGRTLLD+IRDEEPS  KGLFGK
Sbjct: 1   MSLLEEGRSLAGYTLDDVLANNRFGPPASATARAAEIRGRTLLDVIRDEEPSAAKGLFGK 60

Query: 61  DKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDN 120
           DKRTWKSFR+KLRLKRAGSAWVSTVPIPTSD+P   KRSLMG+R+QVRFNT SS+NS D+
Sbjct: 61  DKRTWKSFREKLRLKRAGSAWVSTVPIPTSDMPFQPKRSLMGKRSQVRFNTPSSRNSTDH 120

Query: 121 QSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRH 180
            +R+PSEE+SKS RRQ+ RQ+STMNSSSDS+ F YDES +YSS PD+AP R MRPQMSRH
Sbjct: 121 SARNPSEESSKSFRRQVSRQSSTMNSSSDSTPFQYDESFDYSSVPDSAPSR-MRPQMSRH 180

Query: 181 NSVRVSNPMENYTDPTEPDRDNRIRHLN--LSEHRMMSAREAVAAQEAADAASAAAAAAA 240
           NS+R+ NP ENYTDPTE DRDNR+RH++   SE RMMSARE VAAQEAADAA+AAAAA A
Sbjct: 181 NSIRIPNPTENYTDPTESDRDNRVRHISSAFSERRMMSARETVAAQEAADAAAAAAAAEA 240

Query: 241 AAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREM 300
           A E  E+EK++ +  NE  +ENE  ESTT S EE E+ SP SEPVRMSLMDLLHETDREM
Sbjct: 241 AEEEKEKEKEKEQIENE--DENENVESTTGS-EEWEKVSPTSEPVRMSLMDLLHETDREM 300

Query: 301 GFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRL 360
           GFE S+YGMGFE +   ++ED  YEEDEDDG+G EFSCCVCMVKHKN P+A CGHTFCRL
Sbjct: 301 GFEGSNYGMGFEEDGFSDEEDVAYEEDEDDGSGGEFSCCVCMVKHKNAPMAPCGHTFCRL 360

Query: 361 CSKELMVSRGNCPTCSNFILEILDAF 383
           CSKELMVSRGNCPTC++FILE+L+AF
Sbjct: 361 CSKELMVSRGNCPTCNDFILEMLEAF 382

BLAST of CSPI03G27520.1 vs. ExPASy TrEMBL
Match: A0A6J1ESA4 (uncharacterized protein LOC111437281 OS=Cucurbita moschata OX=3662 GN=LOC111437281 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.3e-144
Identity = 292/387 (75.45%), Postives = 334/387 (86.30%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPP--ATARATEIRGRTLLDIIRDEEPSTGKGLFGK 60
           M +VE+GRSLAGYTLDDVLANNR GPP  AT+RA EIRGRTLLD+IRDEEP+  KGLFGK
Sbjct: 1   MPLVEEGRSLAGYTLDDVLANNRFGPPASATSRAAEIRGRTLLDVIRDEEPNAAKGLFGK 60

Query: 61  DKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDN 120
           DKRTWKSFR+KLRLKRAGSAWVSTVPIPTSD+P   KRSLMG+R+QVRFNT SS+NS D+
Sbjct: 61  DKRTWKSFREKLRLKRAGSAWVSTVPIPTSDMPFQPKRSLMGKRSQVRFNTPSSRNSTDH 120

Query: 121 QSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRH 180
            +R+PSEE+SKS RRQ+ RQ+STMNSSSDS+ F YDES +YSS PD+AP R MRPQMSRH
Sbjct: 121 SARNPSEESSKSIRRQVSRQSSTMNSSSDSTPFQYDESFDYSSIPDSAPSR-MRPQMSRH 180

Query: 181 NSVRVSNPMENYTDPTEPDRDNRIRHLN--LSEHRMMSAREAVAAQEAADAASAAAAAAA 240
           NS+R+ NP ENYTDPTE DRDNR+RH++   SE RMMSARE VAAQEAAD    AAAAAA
Sbjct: 181 NSIRIPNPTENYTDPTESDRDNRVRHISSAFSERRMMSARETVAAQEAAD----AAAAAA 240

Query: 241 AAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREM 300
           AAEAAEEEK++ +  NE+ +E+E  ESTT SEE E+E SP SEPVRMSLMDLLHETDREM
Sbjct: 241 AAEAAEEEKEKEKEQNENEDEDENVESTTGSEEGEKE-SPTSEPVRMSLMDLLHETDREM 300

Query: 301 GFERSSYGMGFEGNFLEEDEDDEY-EEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCR 360
           GFE S+YGMGFE +   ++ED  Y EEDEDDG+G EFSCCVCMVKHKN P+A+CGHTFCR
Sbjct: 301 GFEGSNYGMGFEEDGFSDEEDVAYEEEDEDDGSGGEFSCCVCMVKHKNAPMAACGHTFCR 360

Query: 361 LCSKELMVSRGNCPTCSNFILEILDAF 383
           LCSKELMVSRGNCPTC++FILE+L+AF
Sbjct: 361 LCSKELMVSRGNCPTCNDFILEMLEAF 381

BLAST of CSPI03G27520.1 vs. NCBI nr
Match: XP_011651651.1 (protein FAM9A [Cucumis sativus] >KGN58363.1 hypothetical protein Csa_000963 [Cucumis sativus])

HSP 1 Score: 718.0 bits (1852), Expect = 4.3e-203
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS
Sbjct: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240
           VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240

Query: 241 AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER 300
           AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER
Sbjct: 241 AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER 300

Query: 301 SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE 360
           SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE
Sbjct: 301 SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE 360

Query: 361 LMVSRGNCPTCSNFILEILDAF 383
           LMVSRGNCPTCSNFILEILDAF
Sbjct: 361 LMVSRGNCPTCSNFILEILDAF 382

BLAST of CSPI03G27520.1 vs. NCBI nr
Match: KAA0053347.1 (E3 ubiquitin-protein ligase cblA-like [Cucumis melo var. makuwa] >TYK29191.1 E3 ubiquitin-protein ligase cblA-like [Cucumis melo var. makuwa])

HSP 1 Score: 659.1 bits (1699), Expect = 2.4e-185
Identity = 358/383 (93.47%), Postives = 366/383 (95.56%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPS GKGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPV  KRSL+GRRNQ+RFNTGSSKNS DN S
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           RHPSEE+SKSTRRQM RQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAAS-AAAAAAAAAE 240
           VRVSNPMENYTDPTEPDRDNRIRH NLS+HRMMSAREAVAAQEAADAAS AAAAAAAAAE
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHSNLSDHRMMSAREAVAAQEAADAASAAAAAAAAAAE 240

Query: 241 AAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFE 300
           AAEEEKKQNENNN +GNENE+EESTT S E EEESSPASEPVRMSLMDLLHETDREMGFE
Sbjct: 241 AAEEEKKQNENNNLNGNENEKEESTTGS-EGEEESSPASEPVRMSLMDLLHETDREMGFE 300

Query: 301 RSSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSK 360
           RSSYGMGFE NF E+DEDDEYEEDED+GNG EFSCCVCMVKHKN PLA CGHTFCRLCSK
Sbjct: 301 RSSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCRLCSK 360

Query: 361 ELMVSRGNCPTCSNFILEILDAF 383
           ELMVSRGNCPTCSNFILEILDAF
Sbjct: 361 ELMVSRGNCPTCSNFILEILDAF 382

BLAST of CSPI03G27520.1 vs. NCBI nr
Match: XP_008465469.2 (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase cblA-like [Cucumis melo])

HSP 1 Score: 652.9 bits (1683), Expect = 1.7e-183
Identity = 352/382 (92.15%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPS GKGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPV  KRSL+GRRNQ+RFNTGSSKNS DN S
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           RHPSEE+SKSTRRQM RQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240
           VRVSNPMENYTDPTEPDRDNRIRH NLS+HRMMSAREAVAAQEAADAASAAAAAAAAA +
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHSNLSDHRMMSAREAVAAQEAADAASAAAAAAAAAGS 240

Query: 241 AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER 300
           +   KKQNENNN +GNENE+EESTT S E EEESSPASEPVRMSLMDLLHETDREMGFER
Sbjct: 241 SRRRKKQNENNNLNGNENEKEESTTGS-EGEEESSPASEPVRMSLMDLLHETDREMGFER 300

Query: 301 SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE 360
           SSYGMGFE NF E+DEDDEYEEDED+GNG EFSCCVCMVKHKN PLA CGHTFCRLCSKE
Sbjct: 301 SSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCRLCSKE 360

Query: 361 LMVSRGNCPTCSNFILEILDAF 383
           LMVSRGNCPTCSNFILEILDAF
Sbjct: 361 LMVSRGNCPTCSNFILEILDAF 381

BLAST of CSPI03G27520.1 vs. NCBI nr
Match: XP_038889041.1 (homeobox protein 9 [Benincasa hispida])

HSP 1 Score: 620.9 bits (1600), Expect = 7.2e-174
Identity = 336/382 (87.96%), Postives = 356/382 (93.19%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPATARA EIRGRTLLDIIRDEEPS GKGLFGKDK
Sbjct: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPATARAAEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVH KRSL+GRR QVRFNT SS+NS DNQ+
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHTKRSLIGRRTQVRFNTHSSRNSTDNQT 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           R+PSEE+SKSTRRQM RQTSTMNSSSDSS  HYDESS+YSSSPDAAP RIMRPQMSRHNS
Sbjct: 121 RNPSEESSKSTRRQMSRQTSTMNSSSDSSGSHYDESSDYSSSPDAAPTRIMRPQMSRHNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240
           VRVSNPME+YT+PTEPDRDNRIR+ N S+HRMMSAREAVAAQEAADAASAAAAAAAAA A
Sbjct: 181 VRVSNPMESYTEPTEPDRDNRIRYSNFSDHRMMSAREAVAAQEAADAASAAAAAAAAAVA 240

Query: 241 AEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREMGFER 300
           AEEEK+QNE NNE+GNENE EESTT S  E EESSP +EPVRMSLMDLLHETDREMGFER
Sbjct: 241 AEEEKEQNE-NNENGNENENEESTTGS--ENEESSPTTEPVRMSLMDLLHETDREMGFER 300

Query: 301 SSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFCRLCSKE 360
           SSYGMGFE +F ++DEDDEYEEDEDDG G EF+CCVCMVKHKN PLA+CGHTFCRLCSKE
Sbjct: 301 SSYGMGFEEDFFDDDEDDEYEEDEDDGTGGEFTCCVCMVKHKNPPLAACGHTFCRLCSKE 360

Query: 361 LMVSRGNCPTCSNFILEILDAF 383
           +MVSRGNCPTCSNFI EILDAF
Sbjct: 361 MMVSRGNCPTCSNFISEILDAF 379

BLAST of CSPI03G27520.1 vs. NCBI nr
Match: KAG7011329.1 (hypothetical protein SDJN02_26233, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 532.7 bits (1371), Expect = 2.6e-147
Identity = 295/388 (76.03%), Postives = 332/388 (85.57%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPA +RA EIRGRTLLDIIRDEEPS GKGLFGKD+
Sbjct: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAASRAAEIRGRTLLDIIRDEEPSVGKGLFGKDR 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPV +KRS  G+RNQVRF+   SKNS D+ +
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQDKRSTAGKRNQVRFSVSPSKNSADSST 120

Query: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180
           R PSEE+SKSTRRQM RQTS +N+ +D+SAFHYDES +YSS PDAAP R M+PQMSR NS
Sbjct: 121 R-PSEESSKSTRRQMSRQTSRLNNPNDASAFHYDESFDYSSVPDAAPNRTMKPQMSRRNS 180

Query: 181 VRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAAAAEA 240
           +RVS    +YTDPTEPDRDNR RH N SE RMMSAREAVAAQEAAD      AAAAAAEA
Sbjct: 181 IRVS--AMDYTDPTEPDRDNRARHFNFSESRMMSAREAVAAQEAAD------AAAAAAEA 240

Query: 241 AEEEKKQNENN-NESGNENEEEESTTES-----EEEEEESSPASEPVRMSLMDLLHETDR 300
            E++ +QNENN  E  NENE+E+STT S     EEEEEE+SP+SEPVR+SLMDLLHETD+
Sbjct: 241 TEKDNEQNENNEKEDENENEKEDSTTASEKEKEEEEEEENSPSSEPVRISLMDLLHETDK 300

Query: 301 EMGFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTFC 360
           EMGF  S+YGMGF  +F ++D+D++Y+EDEDDG+G EF+CCVCMVKHKN  LA CGHTFC
Sbjct: 301 EMGFGGSNYGMGFGEDFFDDDDDEDYDEDEDDGHGGEFTCCVCMVKHKNAALAPCGHTFC 360

Query: 361 RLCSKELMVSRGNCPTCSNFILEILDAF 383
           RLCSKE MVSRGNCPTCS+FILEILDAF
Sbjct: 361 RLCSKEFMVSRGNCPTCSDFILEILDAF 379

BLAST of CSPI03G27520.1 vs. TAIR 10
Match: AT3G07120.1 (RING/U-box superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 5.7e-52
Identity = 167/392 (42.60%), Postives = 213/392 (54.34%), Query Frame = 0

Query: 9   SLAGYTLDDVLANNRL-------GPPATARATEIRGR----TLLDIIRDEEPSTGKGLFG 68
           SLAG +LD VL    L        PP     + +  R    TL DIIR+E    G     
Sbjct: 10  SLAGLSLDAVLGGEILIEPPSLPSPPPLPSKSLVPHRPTSQTLFDIIREEYAKEGH---- 69

Query: 69  KDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSS-KNSP 128
           KD+ TW+ FR+KLRLKR GSAW S++ IP SDI + N + +        F + S+  N  
Sbjct: 70  KDRTTWQIFREKLRLKRTGSAWTSSLHIPASDILIPNPKHI-----GTAFRSHSAGLNIR 129

Query: 129 DNQSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMS 188
           D     P  +   S+ R M  + S+M   S  +    D+S + S   D  P R  +PQ+S
Sbjct: 130 DLVHAIPMSDPPGSSGRAMFTRGSSMRVGSSKNP---DDSPDISVLEDGPPSRSFKPQLS 189

Query: 189 RHNSVRVSNPMENYTDPTEPDRDNRIRH--LNLSEHRMMSAREAVAAQEAADAASAAAAA 248
           RH+SVR         D +E + +NR RH  +   E R MSAREAVAAQEAA+A +AAA  
Sbjct: 190 RHDSVR---------DHSEGEENNRRRHPIVTFVEERQMSAREAVAAQEAAEAEAAAAGG 249

Query: 249 AAAAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVR-MSLMDLLHETD 308
                              S +E++++E     E EE +SS ASEP + MSLMDLL ETD
Sbjct: 250 -------------------SEDEDDDDEEDDSGETEEMKSSSASEPKQTMSLMDLLEETD 309

Query: 309 REMGFERSSYGMGFEGNFLEEDEDDEYEEDEDD--GNGE-EFSCCVCMVKHKNGPLASCG 368
           R+MG   S Y M  E    EEDE+DE  E+E D  G GE E SCCVCMVK K      CG
Sbjct: 310 RQMGLTGSRYAMD-EDEEYEEDEEDENNEEEGDSHGGGEGELSCCVCMVKIKGASFTPCG 360

Query: 369 HTFCRLCSKELMVSRGNCPTCSNFILEILDAF 383
           HTFC+LCSKELM  +G+CP CS+F+LE L+ F
Sbjct: 370 HTFCKLCSKELMAQKGHCPVCSSFVLEFLEIF 360

BLAST of CSPI03G27520.1 vs. TAIR 10
Match: AT4G13100.1 (RING/U-box superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 2.7e-17
Identity = 113/394 (28.68%), Postives = 144/394 (36.55%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVL-ANNRLGPPATARATEIRGR---TLLDIIRDEEPSTGKGLF 60
           MS VE+GRS    TL+D+L A        T   T +R     +LLD++R E         
Sbjct: 18  MSTVENGRS--SVTLEDILMAETSSFRSLTTPTTPVRNHSSSSLLDVMRRER-------- 77

Query: 61  GKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSP 120
            +DK  WKS RDKLRLKR  + W+S+ PIPT D             N +         +P
Sbjct: 78  RRDKTAWKSLRDKLRLKRTATGWISSNPIPTLD-------------NHIL--------TP 137

Query: 121 DNQSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMS 180
           DN S                                                        
Sbjct: 138 DNDSH------------------------------------------------------- 197

Query: 181 RHNSVRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAA 240
                                R NR+  L  +     S+R+                + A
Sbjct: 198 ---------------------RFNRLGFLLTNSETNRSSRD---------------VSDA 257

Query: 241 AAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREM 300
           A EAAE E +                 T  + E EE      +P RMSLM+LL + D  M
Sbjct: 258 AEEAAEREGRL-------------RLGTVLAAEREE-----MQPPRMSLMELLEDNDGHM 259

Query: 301 GFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGE--------EFSCCVCMVKHKNGPLAS 360
            +E S+            DE +    D  DG GE        E  CCVCMV+ K      
Sbjct: 318 -YELSA-----------RDEVEVEGRDGCDGGGEAVAVTGAAELGCCVCMVRSKGAAFIP 259

Query: 361 CGHTFCRLCSKELMVSRGNCPTCSNFILEILDAF 383
           CGHTFCRLCS+EL V RGNCP C+  IL++LD F
Sbjct: 378 CGHTFCRLCSRELWVQRGNCPLCNTAILQVLDIF 259

BLAST of CSPI03G27520.1 vs. TAIR 10
Match: AT4G13100.4 (RING/U-box superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 2.7e-17
Identity = 113/394 (28.68%), Postives = 144/394 (36.55%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVL-ANNRLGPPATARATEIRGR---TLLDIIRDEEPSTGKGLF 60
           MS VE+GRS    TL+D+L A        T   T +R     +LLD++R E         
Sbjct: 15  MSTVENGRS--SVTLEDILMAETSSFRSLTTPTTPVRNHSSSSLLDVMRRER-------- 74

Query: 61  GKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSP 120
            +DK  WKS RDKLRLKR  + W+S+ PIPT D             N +         +P
Sbjct: 75  RRDKTAWKSLRDKLRLKRTATGWISSNPIPTLD-------------NHIL--------TP 134

Query: 121 DNQSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMS 180
           DN S                                                        
Sbjct: 135 DNDSH------------------------------------------------------- 194

Query: 181 RHNSVRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAA 240
                                R NR+  L  +     S+R+                + A
Sbjct: 195 ---------------------RFNRLGFLLTNSETNRSSRD---------------VSDA 254

Query: 241 AAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREM 300
           A EAAE E +                 T  + E EE      +P RMSLM+LL + D  M
Sbjct: 255 AEEAAEREGRL-------------RLGTVLAAEREE-----MQPPRMSLMELLEDNDGHM 256

Query: 301 GFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGE--------EFSCCVCMVKHKNGPLAS 360
            +E S+            DE +    D  DG GE        E  CCVCMV+ K      
Sbjct: 315 -YELSA-----------RDEVEVEGRDGCDGGGEAVAVTGAAELGCCVCMVRSKGAAFIP 256

Query: 361 CGHTFCRLCSKELMVSRGNCPTCSNFILEILDAF 383
           CGHTFCRLCS+EL V RGNCP C+  IL++LD F
Sbjct: 375 CGHTFCRLCSRELWVQRGNCPLCNTAILQVLDIF 256

BLAST of CSPI03G27520.1 vs. TAIR 10
Match: AT4G13100.3 (RING/U-box superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.0e-16
Identity = 112/394 (28.43%), Postives = 142/394 (36.04%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVL-ANNRLGPPATARATEIRGR---TLLDIIRDEEPSTGKGLF 60
           MS VE+GRS    TL+D+L A        T   T +R     +LLD++R E         
Sbjct: 18  MSTVENGRS--SVTLEDILMAETSSFRSLTTPTTPVRNHSSSSLLDVMRRER-------- 77

Query: 61  GKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSP 120
            +DK  WKS RDKLRLKR  + W+S+ PIPT D             N +         +P
Sbjct: 78  RRDKTAWKSLRDKLRLKRTATGWISSNPIPTLD-------------NHIL--------TP 137

Query: 121 DNQSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMS 180
           DN S                                                        
Sbjct: 138 DNDSH------------------------------------------------------- 197

Query: 181 RHNSVRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAA 240
                                R NR+  L  +     S+R+                + A
Sbjct: 198 ---------------------RFNRLGFLLTNSETNRSSRD---------------VSDA 256

Query: 241 AAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREM 300
           A EAAE E +                 T  + E         EP RMSLM+LL + D  M
Sbjct: 258 AEEAAEREGRL-------------RLGTVLAAER--------EPPRMSLMELLEDNDGHM 256

Query: 301 GFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGE--------EFSCCVCMVKHKNGPLAS 360
            +E S+            DE +    D  DG GE        E  CCVCMV+ K      
Sbjct: 318 -YELSA-----------RDEVEVEGRDGCDGGGEAVAVTGAAELGCCVCMVRSKGAAFIP 256

Query: 361 CGHTFCRLCSKELMVSRGNCPTCSNFILEILDAF 383
           CGHTFCRLCS+EL V RGNCP C+  IL++LD F
Sbjct: 378 CGHTFCRLCSRELWVQRGNCPLCNTAILQVLDIF 256

BLAST of CSPI03G27520.1 vs. TAIR 10
Match: AT4G13100.5 (RING/U-box superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.0e-16
Identity = 112/394 (28.43%), Postives = 142/394 (36.04%), Query Frame = 0

Query: 1   MSMVEDGRSLAGYTLDDVL-ANNRLGPPATARATEIRGR---TLLDIIRDEEPSTGKGLF 60
           MS VE+GRS    TL+D+L A        T   T +R     +LLD++R E         
Sbjct: 18  MSTVENGRS--SVTLEDILMAETSSFRSLTTPTTPVRNHSSSSLLDVMRRER-------- 77

Query: 61  GKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSP 120
            +DK  WKS RDKLRLKR  + W+S+ PIPT D             N +         +P
Sbjct: 78  RRDKTAWKSLRDKLRLKRTATGWISSNPIPTLD-------------NHIL--------TP 137

Query: 121 DNQSRHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMS 180
           DN S                                                        
Sbjct: 138 DNDSH------------------------------------------------------- 197

Query: 181 RHNSVRVSNPMENYTDPTEPDRDNRIRHLNLSEHRMMSAREAVAAQEAADAASAAAAAAA 240
                                R NR+  L  +     S+R+                + A
Sbjct: 198 ---------------------RFNRLGFLLTNSETNRSSRD---------------VSDA 256

Query: 241 AAEAAEEEKKQNENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETDREM 300
           A EAAE E +                 T  + E         EP RMSLM+LL + D  M
Sbjct: 258 AEEAAEREGRL-------------RLGTVLAAER--------EPPRMSLMELLEDNDGHM 256

Query: 301 GFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGE--------EFSCCVCMVKHKNGPLAS 360
            +E S+            DE +    D  DG GE        E  CCVCMV+ K      
Sbjct: 318 -YELSA-----------RDEVEVEGRDGCDGGGEAVAVTGAAELGCCVCMVRSKGAAFIP 256

Query: 361 CGHTFCRLCSKELMVSRGNCPTCSNFILEILDAF 383
           CGHTFCRLCS+EL V RGNCP C+  IL++LD F
Sbjct: 378 CGHTFCRLCSRELWVQRGNCPLCNTAILQVLDIF 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L9442.1e-203100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G628230 P... [more]
A0A5D3E0G91.2e-18593.47E3 ubiquitin-protein ligase cblA-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CNY78.3e-18492.15LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase cblA-like OS=Cucumis melo OX=36... [more]
A0A6J1K5D61.3e-14475.65E3 ubiquitin-protein ligase rnf8-A-like OS=Cucurbita maxima OX=3661 GN=LOC111491... [more]
A0A6J1ESA41.3e-14475.45uncharacterized protein LOC111437281 OS=Cucurbita moschata OX=3662 GN=LOC1114372... [more]
Match NameE-valueIdentityDescription
XP_011651651.14.3e-203100.00protein FAM9A [Cucumis sativus] >KGN58363.1 hypothetical protein Csa_000963 [Cuc... [more]
KAA0053347.12.4e-18593.47E3 ubiquitin-protein ligase cblA-like [Cucumis melo var. makuwa] >TYK29191.1 E3 ... [more]
XP_008465469.21.7e-18392.15PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase cblA-like [Cucumis m... [more]
XP_038889041.17.2e-17487.96homeobox protein 9 [Benincasa hispida][more]
KAG7011329.12.6e-14776.03hypothetical protein SDJN02_26233, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G07120.15.7e-5242.60RING/U-box superfamily protein [more]
AT4G13100.12.7e-1728.68RING/U-box superfamily protein [more]
AT4G13100.42.7e-1728.68RING/U-box superfamily protein [more]
AT4G13100.31.0e-1628.43RING/U-box superfamily protein [more]
AT4G13100.51.0e-1628.43RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 224..271
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 331..375
e-value: 2.5E-7
score: 30.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 239..253
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 254..275
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..200
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 177..191
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..282
NoneNo IPR availablePANTHERPTHR46629OS01G0917900 PROTEINcoord: 6..382
NoneNo IPR availablePANTHERPTHR46629:SF13OS01G0917900 PROTEINcoord: 6..382
NoneNo IPR availableCDDcd16449RING-HCcoord: 334..371
e-value: 7.41259E-8
score: 46.3065
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 333..378
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 309..382
e-value: 2.9E-10
score: 41.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 334..372
score: 10.600894

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI03G27520CSPI03G27520gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G27520.1.utr5p1CSPI03G27520.1.utr5p1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI03G27520.1.cds1CSPI03G27520.1.cds1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI03G27520.1CSPI03G27520.1-proteinpolypeptide