CSPI03G22960.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G22960.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	Chr3: 19722849 .. 19723941 (-)
Sequence length	875
RNA-Seq Expression	CSPI03G22960.1
Synteny	CSPI03G22960.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATACAAGGACTCTCACCTTCTTTCCTTCCTTGCCCATCAAGGCACTCTGATTCAACGCTCATGTCCCCACACCTCCCAACAAAATGGAAGAGCTGAACGCAAACATTGTTACATTCTAGACTCTGTTCGCGCTCAACTTTTGTTTGTTGCCTGTCTTGAAAAATTTTGGGGAGAGGTTGCCCCACCTCTGTTTATGTCATCAACTGTCTTCCTTCTCAAGTCATTCACAATGTTTCCCCATTTGAACGGCTATATGGTATTTTTTCCTCTTACTCTAGTCTCAAGGTTTTTGGTTGTGCATGTGTTGTGTTATTACATTCTCATGAACATACCAAACTTGAATCATGTGCTCGTCTATGTGGTTTCTTAGGTTATGGTACTAAACATAAAGGATTTCGTTGTTGGGATCCCATCTCTCAACGATTACGTAGATCTCGTCCTGTCACCTTTTGGGAACATCGTATGTTATCTAGTCTTTCTTTATTTCATGCATCTCTCTCTAGTCATCATCCATTCTTCACTGATCTGTGTCTTCTTCGTCGTGCTTTCTATGGTCTCAAATAGGCTCCTCGAGCTTGGTTTGCAACATTCAGTTCCACTATTACTCAACTTGGGTTCACCTCCAGCTCTCATGATTCGATCTTATTCACTCGTCAGACATCTCGTGGTATTGTGCTCCTTCTTTTATATGTTGATGGTATGATTATTACAGGTGATGATCCTCAAGTCATGTCTGATTTGCAATGTTACCTGAGTAAGCACTTTGAGATTAAGGATTTAGGAACCCTCAGTTATTTTCTTGGCCTTGAGATCTCATCCTCATATGATGGCTACTACATATCTCAAGGTAAATATGCATCTAACCTCCTCAGACGATCTGGTATTACCGACTCTACCACATCCTCAACGCCTTTAGATCCAAATGTGTGACTCACTCCTTTTGATGGTATTCCTCTTGACGATCCTACTTTGTATCGACAACTTGTTGGTAGCTTGATTTACTTAAATGTGACTCGTTCAGATATTGCATTTGCAGTTCACATTATTAGTTTATTCATGGCTGCTCCTCGTACTATTTACTTCACTGT

mRNA sequence

ATGGAATACAAGGACTCTCACCTTCTTTCCTTCCTTGCCCATCAAGGCACTCTGATTCAACGCTCATGTCCCCACACCTCCCAACAAAATGGAAGAGCTGAACGCAAACATTGTTACATTCTAGACTCTGTTCGCGCTCAACTTTTGTTTGTTGCCTGTCTTGAAAAATTTTGGGGAGAGGTTGCCCCACCTCTGTTTATGTCATCAACTGTCTTCCTTCTCAAGTCATTCACAATGTTTCCCCATTTGAACGGCTATATGGTTATGGTACTAAACATAAAGGATTTCGTTGTTGGGATCCCATCTCTCAACGATTACGTAGATCTCGTCCTGTCACCTTTTGGGAACATCGCTCCTCGAGCTTGGTTTGCAACATTCAGTTCCACTATTACTCAACTTGGGTTCACCTCCAGCTCTCATGATTCGATCTTATTCACTCGTCAGACATCTCGTGGTATTGTGCTCCTTCTTTTATATGTTGATGGTATGATTATTACAGGTGATGATCCTCAAGTCATGTCTGATTTGCAATGTTACCTGAGTAAGCACTTTGAGATTAAGGATTTAGGAACCCTCAGTTATTTTCTTGGCCTTGAGATCTCATCCTCATATGATGGCTACTACATATCTCAAGGTAAATATGCATCTAACCTCCTCAGACGATCTGGTATTACCGACTCTACCACATCCTCAACGCCTTTAGATCCAAATGTGTGACTCACTCCTTTTGATGGTATTCCTCTTGACGATCCTACTTTGTATCGACAACTTGTTGGTAGCTTGATTTACTTAAATGTGACTCGTTCAGATATTGCATTTGCAGTTCACATTATTAGTTTATTCATGGCTGCTCCTCGTACTATTTACTTCACTGT

Coding sequence (CDS)

ATGGAATACAAGGACTCTCACCTTCTTTCCTTCCTTGCCCATCAAGGCACTCTGATTCAACGCTCATGTCCCCACACCTCCCAACAAAATGGAAGAGCTGAACGCAAACATTGTTACATTCTAGACTCTGTTCGCGCTCAACTTTTGTTTGTTGCCTGTCTTGAAAAATTTTGGGGAGAGGTTGCCCCACCTCTGTTTATGTCATCAACTGTCTTCCTTCTCAAGTCATTCACAATGTTTCCCCATTTGAACGGCTATATGGTTATGGTACTAAACATAAAGGATTTCGTTGTTGGGATCCCATCTCTCAACGATTACGTAGATCTCGTCCTGTCACCTTTTGGGAACATCGCTCCTCGAGCTTGGTTTGCAACATTCAGTTCCACTATTACTCAACTTGGGTTCACCTCCAGCTCTCATGATTCGATCTTATTCACTCGTCAGACATCTCGTGGTATTGTGCTCCTTCTTTTATATGTTGATGGTATGATTATTACAGGTGATGATCCTCAAGTCATGTCTGATTTGCAATGTTACCTGAGTAAGCACTTTGAGATTAAGGATTTAGGAACCCTCAGTTATTTTCTTGGCCTTGAGATCTCATCCTCATATGATGGCTACTACATATCTCAAGGTAAATATGCATCTAACCTCCTCAGACGATCTGGTATTACCGACTCTACCACATCCTCAACGCCTTTAGATCCAAATGTGTGA

Protein sequence

MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGEVAPPLFMSSTVFLLKSFTMFPHLNGYMVMVLNIKDFVVGIPSLNDYVDLVLSPFGNIAPRAWFATFSSTITQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDLQCYLSKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPLDPNV*

Homology

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.4e-16
Identity = 44/120 (36.67%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 118  APRAWFATFSSTITQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDLQ 177
            APRAW+    + +  +GF +S  D+ LF  Q  + IV +L+YVD ++ITG+DP ++ +  
Sbjct: 1109 APRAWYVELRNYLLTIGFVNSVSDTSLFVLQRGKSIVYMLVYVDDILITGNDPTLLHNTL 1168

Query: 178  CYLSKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPLDPN 237
              LS+ F +KD   L YFLG+E      G ++SQ +Y  +LL R+ +  +   +TP+ P+
Sbjct: 1169 DNLSQRFSVKDHEELHYFLGIEAKRVPTGLHLSQRRYILDLLARTNMITAKPVTTPMAPS 1228

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 9.8e-15
Identity = 41/117 (35.04%), Postives = 68/117 (58.12%), Query Frame = 0


Query: 118  APRAWFATFSSTITQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDLQ 177
            APRAW+    + +  +GF +S  D+ LF  Q  R I+ +L+YVD ++ITG+D  ++    
Sbjct: 1092 APRAWYVELRTYLLTVGFVNSISDTSLFVLQRGRSIIYMLVYVDDILITGNDTVLLKHTL 1151

Query: 178  CYLSKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPL 235
              LS+ F +K+   L YFLG+E      G ++SQ +Y  +LL R+ +  +   +TP+
Sbjct: 1152 DALSQRFSVKEHEDLHYFLGIEAKRVPQGLHLSQRRYTLDLLARTNMLTAKPVATPM 1208

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy Swiss-Prot
Match: P92519 (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.6e-11
Identity = 34/79 (43.04%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 156 LLLYVDGMIITGDDPQVMSDLQCYLSKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYA 215
           LLLYVD +++TG    +++ L   LS  F +KDLG + YFLG++I +   G ++SQ KYA
Sbjct: 3   LLLYVDDILLTGSSNTLLNMLIFQLSSTFSMKDLGPVHYFLGIQIKTHPSGLFLSQTKYA 62

Query: 216 SNLLRRSGITDSTTSSTPL 235
             +L  +G+ D    STPL
Sbjct: 63  EQILNNAGMLDCKPMSTPL 81

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.6e-11
Identity = 42/120 (35.00%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 118  APRAWFATFSSTI-TQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDL 177
            APR W+  F S + +Q    + S   + F R +    ++LLLYVD M+I G D  +++ L
Sbjct: 966  APRQWYMKFDSFMKSQTYLKTYSDPCVYFKRFSENNFIILLLYVDDMLIVGKDKGLIAKL 1025

Query: 178  QCYLSKHFEIKDLGTLSYFLGLEI--SSSYDGYYISQGKYASNLLRRSGITDSTTSSTPL 235
            +  LSK F++KDLG     LG++I    +    ++SQ KY   +L R  + ++   STPL
Sbjct: 1026 KGDLSKSFDMKDLGPAQQILGMKIVRERTSRKLWLSQEKYIERVLERFNMKNAKPVSTPL 1085

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 65.9 bits (159), Expect = 7.3e-10
Identity = 35/123 (28.46%), Postives = 60/123 (48.78%), Query Frame = 0


Query: 118  APRAWFATFSSTITQLGFTSSSHDSILF--TRQTSRGIVLLLLYVDGMIITGDDPQVMSD 177
            A R WF  F   + +  F +SS D  ++   +      + +LLYVD ++I   D   M++
Sbjct: 1044 AARCWFEVFEQALKECEFVNSSVDRCIYILDKGNINENIYVLLYVDDVVIATGDMTRMNN 1103

Query: 178  LQCYLSKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPLD 237
             + YL + F + DL  + +F+G+ I    D  Y+SQ  Y   +L +  + +    STPL 
Sbjct: 1104 FKRYLMEKFRMTDLNEIKHFIGIRIEMQEDKIYLSQSAYVKKILSKFNMENCNAVSTPLP 1163

Query: 238  PNV 239
              +
Sbjct: 1164 SKI 1166

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BLAST of CSPI03G22960.1 vs. ExPASy TrEMBL
Match: A0A5A7TGJ6 (Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G00960 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 2.3e-59
Identity = 170/399 (42.61%), Postives = 185/399 (46.37%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           MEYKDS  LSFLA QGTLIQRSCPHTSQQNGRAERKH +ILDSV AQLL  +C EKFWGE
Sbjct: 291 MEYKDSRFLSFLAQQGTLIQRSCPHTSQQNGRAERKHRHILDSVLAQLLSGSCPEKFWGE 350

Query: 61  VA-----------PPLFMSSTVFLLKSFTMFP--------------------HLNGY--- 120
            A             L  S T   L      P                    HL  Y   
Sbjct: 351 AALTSIYVINRLPSQLTESHTTSALPDLPSVPCEEPEHAPIRRSTRVRETPSHLKEYHCF 410

Query: 121 -----MVMVLNIKDFVVG----------IPSLN-----DYVDL----------------- 180
                +V   + K+              + +L      DYVDL                 
Sbjct: 411 STIMSLVEPSSYKEASTNPLWKQAMDNELQALTKTHTWDYVDLPPGKKPIGCKWIFKIKT 470

Query: 181 ----------------------------VLSPFGNI------------------------ 239
                                         +P   +                        
Sbjct: 471 HSDGSIERYKARLVVKGYSQEYGIDYEETFAPVARMTSVRSLLAIAAAKQWPLLQMDVKN 530

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy TrEMBL
Match: A0A5A7UST7 (Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005430 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 2.4e-56
Identity = 159/370 (42.97%), Postives = 181/370 (48.92%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           MEYKDS  LSFLA QGTLIQRSCPHT QQNGRAERKH +ILDSV  QLL  +C E FWGE
Sbjct: 368 MEYKDSRFLSFLAQQGTLIQRSCPHTFQQNGRAERKHRHILDSVHVQLLSRSCSENFWGE 427

Query: 61  VA---------------------------PPLFMSSTVFLLKSFTMF-PHLN-------- 120
            A                           PP +    VF    F +  PH +        
Sbjct: 428 AALTSVYVINRLPSQVIHNLSPFERLYDTPPSYSDLKVFGCACFVLLHPHEHTKPKPRAH 487

Query: 121 -------------------------------------------GYMVMVLNIKD------ 180
                                                      G M+  L +K+      
Sbjct: 488 LCCFLGYGTEHKAEPSSYKEASTNPLWQQAMDDELQALAKLTPGIMLTYLLVKNLLAVKT 547

Query: 181 --------FVVGIPSL--------------------NDYVDLVLSP-------------- 239
                   FV  + ++                      Y ++ + P              
Sbjct: 548 FALVAQMTFVRSLLAIAAAKQWHLLQMDVKNVFLNGTIYEEVYMKPPPGTTPPPQKVCLL 607

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy TrEMBL
Match: A0A5D3CPU5 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G00700 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 9.5e-53
Identity = 168/450 (37.33%), Postives = 189/450 (42.00%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           M+YKDS  LSFL  QGTLIQRSCPHTSQQNGRAERKH +ILDSVRAQLL  +C + FWGE
Sbjct: 451 MKYKDSRFLSFLTQQGTLIQRSCPHTSQQNGRAERKHRHILDSVRAQLLSASCPKNFWGE 510

Query: 61  VA---------------------------PPLFMSSTVF--------------------- 120
            A                           PP + +  +F                     
Sbjct: 511 AAITSVYVINRLPSQVIHNISPFERLHGTPPSYSNLKIFGCECFVLLHPHEHTKLEPRAR 570

Query: 121 ---------------------------------------LLKSF---------------- 180
                                                   L SF                
Sbjct: 571 LCCFLGYGTEHKGFRCWDPISQRLRISRHVTFWEHRMFSSLSSFHASLSSSQSFFTDPST 630

Query: 181 TMFP---------------------------HLNGYMVMVLNIKDFVVGIPSL------- 239
           T+FP                           HL+    + L + D  +G   L       
Sbjct: 631 TLFPTPDSPPNTIPYPPLSSELTPSLRSRIFHLSPLRNLNLRLSDDPLGTNPLWQQAMND 690

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy TrEMBL
Match: A0A5A7UCC7 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold578G00790 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 4.7e-52
Identity = 164/454 (36.12%), Postives = 188/454 (41.41%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           +EYKDS LLSFL+ QGTL+QRSCPHTSQQNGRAERKH +ILDS RA LL  +C EKFWGE
Sbjct: 496 LEYKDSTLLSFLSQQGTLVQRSCPHTSQQNGRAERKHRHILDSFRALLLSASCPEKFWGE 555

Query: 61  VAPPLFMSSTVFLLKSFTMFPHLNGYMVMVLNI---KDFVVGI----------------- 120
            A    +   VFL  SF    H   YMV++L I   K  VV +                 
Sbjct: 556 AALNPSILLIVFLPSSFKTSLHSKDYMVLLLTIPTLKSLVVLVLFSCILMNTLNLNLVPA 615

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 616 SAVFLAMAQNIKHTMFSRLSSFHTSFSSPQSFFTDTSIDLFPLSESTPDNELAQSAPTSA 675

Query: 181 -------------PSLN----------------DYVDL---------------------- 239
                        PS +                DYVDL                      
Sbjct: 676 TSDQSSISDGNPDPSPDIPPRRSTRALEKTHTWDYVDLPPGKRPIGCKWIYKIKTHSDGT 735

[Close]

BLAST of CSPI03G22960.1 vs. ExPASy TrEMBL
Match: A0A5D3DAW5 (Integrase, catalytic core OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00510 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 7.5e-50
Identity = 158/426 (37.09%), Postives = 180/426 (42.25%), Query Frame = 0


Query: 6   SHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGEVA--- 65
           +  LSFLA QGTLIQRSCPHTSQQNGRAERKH +ILDSVRAQLL  +C EKFWGE A   
Sbjct: 7   TRFLSFLAQQGTLIQRSCPHTSQQNGRAERKHRHILDSVRAQLLSASCSEKFWGEAALTS 66

Query: 66  ------------------------PPLFMSSTVFLLKSFTMF---------PHLN----- 125
                                    P + +  VF    F +          P        
Sbjct: 67  VYVINRLPSQVIYNVSPFEQLYGTSPSYSNLKVFGCACFVLLHSHEHTKLEPRARLCCFL 126

Query: 126 GY----MVMVLNIKDFVVGIPSLN------------------------------------ 185
           GY     V++  ++  ++  P+ N                                    
Sbjct: 127 GYGTEHKVLIHTLRFHLLIYPAPNTTPCPPLTSELTQYHTTSAPPDLPSVSCEEPAPAPV 186

Query: 186 ------------------------------DYVDL--------------------VLSPF 239
                                         DYV+                       +P 
Sbjct: 187 RRSTREASINPLWQQAMNEELQALEKTHTWDYVNFPPGKKPIGCYSQEYDIDYEETFAPI 246

[Close]

BLAST of CSPI03G22960.1 vs. NCBI nr
Match: XP_031738012.1 (uncharacterized protein LOC116402635 [Cucumis sativus] >XP_031738013.1 uncharacterized protein LOC116402635 [Cucumis sativus])

HSP 1 Score: 476.5 bits (1225), Expect = 1.4e-130
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE
Sbjct: 19  MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 78

Query: 61  VAPPLFMSSTVFLLKSFTMFPHLNGYMVMVLNIKDFVVGIPSLNDYVDLVLSPFGNIAPR 120
           VAPPLFMSSTVFLLKSFTMFPHLNGYMVMVLNIKDFVVGIPSLNDYVDLVLSPFGNIAPR
Sbjct: 79  VAPPLFMSSTVFLLKSFTMFPHLNGYMVMVLNIKDFVVGIPSLNDYVDLVLSPFGNIAPR 138

Query: 121 AWFATFSSTITQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDLQCYL 180
           AWFATFSSTITQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDLQCYL
Sbjct: 139 AWFATFSSTITQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDLQCYL 198

Query: 181 SKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPLDPNV 239
           SKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPLDPNV
Sbjct: 199 SKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPLDPNV 256

[Close]

BLAST of CSPI03G22960.1 vs. NCBI nr
Match: KAA0041166.1 (putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 238.8 bits (608), Expect = 4.8e-59
Identity = 170/399 (42.61%), Postives = 185/399 (46.37%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           MEYKDS  LSFLA QGTLIQRSCPHTSQQNGRAERKH +ILDSV AQLL  +C EKFWGE
Sbjct: 291 MEYKDSRFLSFLAQQGTLIQRSCPHTSQQNGRAERKHRHILDSVLAQLLSGSCPEKFWGE 350

Query: 61  VA-----------PPLFMSSTVFLLKSFTMFP--------------------HLNGY--- 120
            A             L  S T   L      P                    HL  Y   
Sbjct: 351 AALTSIYVINRLPSQLTESHTTSALPDLPSVPCEEPEHAPIRRSTRVRETPSHLKEYHCF 410

Query: 121 -----MVMVLNIKDFVVG----------IPSLN-----DYVDL----------------- 180
                +V   + K+              + +L      DYVDL                 
Sbjct: 411 STIMSLVEPSSYKEASTNPLWKQAMDNELQALTKTHTWDYVDLPPGKKPIGCKWIFKIKT 470

Query: 181 ----------------------------VLSPFGNI------------------------ 239
                                         +P   +                        
Sbjct: 471 HSDGSIERYKARLVVKGYSQEYGIDYEETFAPVARMTSVRSLLAIAAAKQWPLLQMDVKN 530

[Close]

BLAST of CSPI03G22960.1 vs. NCBI nr
Match: KAA0058220.1 (putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 228.8 bits (582), Expect = 5.0e-56
Identity = 159/370 (42.97%), Postives = 181/370 (48.92%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           MEYKDS  LSFLA QGTLIQRSCPHT QQNGRAERKH +ILDSV  QLL  +C E FWGE
Sbjct: 368 MEYKDSRFLSFLAQQGTLIQRSCPHTFQQNGRAERKHRHILDSVHVQLLSRSCSENFWGE 427

Query: 61  VA---------------------------PPLFMSSTVFLLKSFTMF-PHLN-------- 120
            A                           PP +    VF    F +  PH +        
Sbjct: 428 AALTSVYVINRLPSQVIHNLSPFERLYDTPPSYSDLKVFGCACFVLLHPHEHTKPKPRAH 487

Query: 121 -------------------------------------------GYMVMVLNIKD------ 180
                                                      G M+  L +K+      
Sbjct: 488 LCCFLGYGTEHKAEPSSYKEASTNPLWQQAMDDELQALAKLTPGIMLTYLLVKNLLAVKT 547

Query: 181 --------FVVGIPSL--------------------NDYVDLVLSP-------------- 239
                   FV  + ++                      Y ++ + P              
Sbjct: 548 FALVAQMTFVRSLLAIAAAKQWHLLQMDVKNVFLNGTIYEEVYMKPPPGTTPPPQKVCLL 607

[Close]

BLAST of CSPI03G22960.1 vs. NCBI nr
Match: KAA0037189.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK13883.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 216.9 bits (551), Expect = 2.0e-52
Identity = 168/450 (37.33%), Postives = 189/450 (42.00%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           M+YKDS  LSFL  QGTLIQRSCPHTSQQNGRAERKH +ILDSVRAQLL  +C + FWGE
Sbjct: 451 MKYKDSRFLSFLTQQGTLIQRSCPHTSQQNGRAERKHRHILDSVRAQLLSASCPKNFWGE 510

Query: 61  VA---------------------------PPLFMSSTVF--------------------- 120
            A                           PP + +  +F                     
Sbjct: 511 AAITSVYVINRLPSQVIHNISPFERLHGTPPSYSNLKIFGCECFVLLHPHEHTKLEPRAR 570

Query: 121 ---------------------------------------LLKSF---------------- 180
                                                   L SF                
Sbjct: 571 LCCFLGYGTEHKGFRCWDPISQRLRISRHVTFWEHRMFSSLSSFHASLSSSQSFFTDPST 630

Query: 181 TMFP---------------------------HLNGYMVMVLNIKDFVVGIPSL------- 239
           T+FP                           HL+    + L + D  +G   L       
Sbjct: 631 TLFPTPDSPPNTIPYPPLSSELTPSLRSRIFHLSPLRNLNLRLSDDPLGTNPLWQQAMND 690

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BLAST of CSPI03G22960.1 vs. NCBI nr
Match: KAA0052097.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 214.5 bits (545), Expect = 9.7e-52
Identity = 164/454 (36.12%), Postives = 188/454 (41.41%), Query Frame = 0


Query: 1   MEYKDSHLLSFLAHQGTLIQRSCPHTSQQNGRAERKHCYILDSVRAQLLFVACLEKFWGE 60
           +EYKDS LLSFL+ QGTL+QRSCPHTSQQNGRAERKH +ILDS RA LL  +C EKFWGE
Sbjct: 496 LEYKDSTLLSFLSQQGTLVQRSCPHTSQQNGRAERKHRHILDSFRALLLSASCPEKFWGE 555

Query: 61  VAPPLFMSSTVFLLKSFTMFPHLNGYMVMVLNI---KDFVVGI----------------- 120
            A    +   VFL  SF    H   YMV++L I   K  VV +                 
Sbjct: 556 AALNPSILLIVFLPSSFKTSLHSKDYMVLLLTIPTLKSLVVLVLFSCILMNTLNLNLVPA 615

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 616 SAVFLAMAQNIKHTMFSRLSSFHTSFSSPQSFFTDTSIDLFPLSESTPDNELAQSAPTSA 675

Query: 181 -------------PSLN----------------DYVDL---------------------- 239
                        PS +                DYVDL                      
Sbjct: 676 TSDQSSISDGNPDPSPDIPPRRSTRALEKTHTWDYVDLPPGKRPIGCKWIYKIKTHSDGT 735

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BLAST of CSPI03G22960.1 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 90.5 bits (223), Expect = 2.0e-18
Identity = 49/121 (40.50%), Postives = 70/121 (57.85%), Query Frame = 0


Query: 118 APRAWFATFSSTITQLGFTSSSHDSILFTRQTSRGIVLLLLYVDGMIITGDDPQVMSDLQ 177
           A R WF  FS T+   GF  S  D   F + T+   + +L+YVD +II  ++   + +L+
Sbjct: 242 ASRQWFLKFSVTLIGFGFVQSHSDHTYFLKITATLFLCVLVYVDDIIICSNNDAAVDELK 301

Query: 178 CYLSKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYASNLLRRSGITDSTTSSTPLDPN 237
             L   F+++DLG L YFLGLEI+ S  G  I Q KYA +LL  +G+     SS P+DP+
Sbjct: 302 SQLKSCFKLRDLGPLKYFLGLEIARSAAGINICQRKYALDLLDETGLLGCKPSSVPMDPS 361

Query: 238 V 239
           V
Sbjct: 362 V 362

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BLAST of CSPI03G22960.1 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 6.1e-12
Identity = 34/79 (43.04%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 156 LLLYVDGMIITGDDPQVMSDLQCYLSKHFEIKDLGTLSYFLGLEISSSYDGYYISQGKYA 215
           LLLYVD +++TG    +++ L   LS  F +KDLG + YFLG++I +   G ++SQ KYA
Sbjct: 3   LLLYVDDILLTGSSNTLLNMLIFQLSSTFSMKDLGPVHYFLGIQIKTHPSGLFLSQTKYA 62

Query: 216 SNLLRRSGITDSTTSSTPL 235
             +L  +G+ D    STPL
Sbjct: 63  EQILNNAGMLDCKPMSTPL 81

The following BLAST results are available for this feature:

BLAST of CSPI03G22960.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94HW2	1.4e-16	36.67	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q9ZT94	9.8e-15	35.04	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
P92519	8.6e-11	43.04	Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 ...	[more]
P10978	8.6e-11	35.00	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	7.3e-10	28.46	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]

BLAST of CSPI03G22960.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TGJ6	2.3e-59	42.61	Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A5A7UST7	2.4e-56	42.97	Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A5D3CPU5	9.5e-53	37.33	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var....	[more]
A0A5A7UCC7	4.7e-52	36.12	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var....	[more]
A0A5D3DAW5	7.5e-50	37.09	Integrase, catalytic core OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of CSPI03G22960.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031738012.1	1.4e-130	100.00	uncharacterized protein LOC116402635 [Cucumis sativus] >XP_031738013.1 uncharact...	[more]
KAA0041166.1	4.8e-59	42.61	putative mitochondrial protein [Cucumis melo var. makuwa]	[more]
KAA0058220.1	5.0e-56	42.97	putative mitochondrial protein [Cucumis melo var. makuwa]	[more]
KAA0037189.1	2.0e-52	37.33	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]
KAA0052097.1	9.7e-52	36.12	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]

BLAST of CSPI03G22960.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23160.1	2.0e-18	40.50	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]
ATMG00810.1	6.1e-12	43.04	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..68 e-value: 2.6E-6 score: 29.2
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 118..234 e-value: 3.9E-23 score: 82.3
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 118..237
None	No IPR available	PANTHER	PTHR11439:SF351	CYSTEINE-RICH RLK (RECEPTOR-LIKE PROTEIN KINASE) 8	coord: 118..237
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 2..59

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G22960	CSPI03G22960	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G22960.1.utr3p1	CSPI03G22960.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G22960.1.cds3	CSPI03G22960.1.cds3	CDS
CSPI03G22960.1.cds2	CSPI03G22960.1.cds2	CDS
CSPI03G22960.1.cds1	CSPI03G22960.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G22960.1	CSPI03G22960.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding